PerkinElmer Lambda XLS User manual

LAMBDA XLS/XLS+
User’s Guide
UV-VIS SPECTROSCOPY

Release History
Part Number Release Publication Date
L6050017 D January 2014
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer Ltd
Chalfont Road
Seer Green
Beaconsfield
BUCKS
HP9 2FX
United Kingdom
Or emailed to: [e-mail]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no
warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in
connection with furnishing, performance or use of this material.
Copyright Information
This document contains proprietary information that is protected by copyright.
All rights are reserved. No part of this publication may be reproduced in any form whatsoever or
translated into any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2014 PerkinElmer, Inc.
Produced in the UK.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically marked as such,
are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.

Table of Contents
Introduction......................................................................................5
About this Manual ....................................................................................6
Conventions Used in this Manual...............................................................7
Notes, cautions and warnings.............................................................7
Safety Information ...........................................................................9
General Safety.......................................................................................10
Decontamination Certificates............................................................11
Chemicals .......................................................................................11
Toxic Fumes....................................................................................11
Waste Disposal................................................................................12
Personal Protective Equipment .........................................................12
Intended User.................................................................................12
Unpacking and Installation......................................................................13
Installing your Instrument .............................................................15
Unpacking .............................................................................................16
Connecting to the Line Power Supply.......................................................17
Using your Instrument....................................................................19
Overview of the Instrument....................................................................20
Switching on the Instrument...................................................................22
Using the Software.................................................................................23
Methods and Favorites ...........................................................................27
Folder Options.................................................................................27
Method Options...............................................................................30
Lambda XLS/XLS+ Instrument Methods...................................................32
Utilities..................................................................................................33
Date and Time ................................................................................34
Regional..........................................................................................34
Printer ............................................................................................35
Preferences.....................................................................................36
Contrast..........................................................................................37
About .............................................................................................37
Sample Handling....................................................................................38
Instrument Methods .......................................................................39
Single Wavelength .................................................................................40
Multi Wavelength ...................................................................................43
Spectrum...............................................................................................46
Concentration ........................................................................................51
Standard Curve......................................................................................56
Kinetics .................................................................................................62
Absorbance Ratio...................................................................................67
Accessories......................................................................................71
Accessories and Consumables.................................................................72
Printer Installation and Configuration ......................................................73
Printer installation............................................................................73
Printer configuration........................................................................74
Loading or changing the printer paper..............................................75
Bluetooth Accessory Installation..............................................................76
Lambda Bio/XLS Print Utility....................................................................79
Maintenance....................................................................................81
General Maintenance..............................................................................82
Cleaning the instrument...................................................................82
Storage and Shipping .............................................................................84

Appendices......................................................................................85
Appendix 1: Specifications.......................................................................86
Appendix 2: Customer Care Contacts.......................................................87
Index.....................................................................................................89

Introduction

6 . Lambda XLS/XLS+ User's Guide
About this Manual
This user’s guide describes the installation and operating procedures for your
Lambda XLS/XLS+ UV/Vis spectrometer.
The manual contains the following sections:
•Introduction;
•Safety Information;
•Installing your Instrument;
•Using your Instrument;
•Instrument Methods;
•Accessories;
•Maintenance;
•Appendices.

Introduction . 7
Conventions Used in this Manual
Normal text is used to provide information and instructions.
Bold text refers to text that is displayed on the screen.
UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. The
symbol “+” is used to show that you have to press two keys at the same time, for example,
ALT+F.
All eight digit numbers are PerkinElmer part numbers unless stated otherwise.
Unless stated otherwise, in this manual “instrument” refers to the Lambda XLS or
Lambda XLS+ instrument.
Notes, cautions and warnings
Three terms, in the following standard formats, are also used to highlight special
circumstances and warnings.
NOTE: A note indicates additional, significant information that is provided with some
procedures.
WARNING
We use the term WARNING to inform you about situations that could
result in
personal injury
to yourself or other persons. Details about
these circumstances are in a box like this one.
CAUTION
We use the term CAUTION to inform you about situations that could
result in
serious damage to the instrument
or other equipment.
Details about these circumstances are in a box like this one.

8 . Lambda XLS/XLS+ User's Guide

Safety Information

10 . Lambda XLS/XLS+ User's Guide
General Safety
The Lambda Bio/XLS instruments conform to the requirements of the Low Voltage Directive
2006/95/EC, EMC Directive 2004/108/EC and the IVD Directive 98/79/EC.
Standards to which conformity is declared:
•EN 61010-1 Safety requirements for electrical equipment for measurement, control and
laboratory use.
•EN 61010-2-101 Particular requirements for IVD medical equipment.
•BS EN 591 Instruction for use for in vitro diagnostic instruments for professional use.
•BS EN 13612 Performance evaluation of in vitro diagnostic medical devices.
•EN 61326-2.3 Electromagnetic compatibility – generic emission standard Electrical
equipment for measurement, control and laboratory use.
•EN 61326 Electromagnetic compatibility – generic emission standard Electrical
equipment for measurement, control and laboratory use. Classified as Class B for
conducted and radiated emissions.
The Lambda Bio/XLS complies with the following environmental legislation regarding the
restriction of the use of certain hazardous substances:
•Europe: EU directive 2002/95/EC (RoHS Directive).
•China: Administration on the Control of Pollution Caused by Electronic Information
Products No 39.
This appliance is marked according to the European directive 2002/96/EC on Waste Electrical
and Electronic Equipment (WEEE). See the
Lambda Bio/XLS Safety Manual (L6050014) for
more information.
There are a number of warning labels and symbols on your instrument. These are there to
inform you where potential danger exists or particular caution is required. Before
commencing installation, please take time to familiarize yourself with these symbols and their
meaning.
This instrument is subject to the following hazards:
The UV source contained within the unit generates a light beam that passes
through
the sample chamber during sample measurements. Under normal use,
this beam is contained within the unit and no UV radiation is emitted from the
system. However, care should be taken that no reflective items are placed in
the beam during sample measurements so that the beam is reflected out of
the system. Also, the sample holder should be
removed for cleaning only when
the power is disconnected from the instrument. Prolonged exposure to the
beam may cause permanent eye damage.

Safety Information . 11
•High voltages exist inside the unit. Repair and maintenance should only be carried out
by individuals trained specifically to work on these instruments.
There are no bio-hazardous materials within the unit; however, this unit could
be used with bio-hazardous samples. Given the general-purpose nature of the
instruments it is not possible to define exact decontamination procedures for
the many types of samples being tested. These should be established by the
user, or their Health and Safety representative, before use of the instrument.
Guidance can be found in document
M29-A3 Protection of Laboratory Workers
from Occupationally Acquired Infections: Approved Guidelines
published by
the US Clinical and Laboratory Standards Institute. The sample compartment
may be removed and cleaned by soaking in a disinfectant appropriate for the
bio-hazard for at least 30 minutes. The compartment should then be rinsed in
distilled water and allowed to dry. Similarly, the outer cover can be wiped over
with an appropriate disinfectant with a wipe or soft cloth. Any internal spills
should be wiped up using absorbent material through the sample compartment
aperture, and then similarly decontaminated using disinfectant soaked wipes.
Decontamination Certificates
If the instrument needs to be returned, a decontamination certificate is required.
Decontamination certificates can be found at the following web address or be provided by
Customer Care.
http://www.perkinelmer.com/LambdaBioXLSSupport
It is the responsibility of the user to ensure a safe working environment is provided to the
engineer or staff by appropriate risk assessments and, if appropriate, decontamination
procedures before work commences.
Chemicals
Use, store, and dispose of chemicals that you require for your analyses in accordance with
the manufacturer’s recommendations and local safety regulations.
Toxic Fumes
If you are working with volatile solvents or toxic substances, then you must provide an
efficient laboratory ventilation system to remove vapors that may be produced when you are
performing analyses.

12 . Lambda XLS/XLS+ User's Guide
Waste Disposal
Waste containers may contain corrosive or organic solutions and small amounts of the
substances that were analyzed. If these materials are toxic, you may have to treat the
collected effluent as hazardous waste. Refer to your local safety regulations for proper
disposal procedures.
Personal Protective Equipment
No Personal Protective Equipment (PPE) is required for operation of the equipment, although
the samples under test may require the use of such PPE and should be covered by a risk
assessment made by the user or the appropriative Health and Safety representative.
Intended User
This instrument is intended for use by individuals trained in and familiar with the use of
spectrophotometers and their associated hazards as detailed above. In the event of a
malfunction or hazard occurring, the user responsible shall disconnect the unit from power,
and isolate the instrument for decontamination by an appropriate means if biohazardous
material is spilled on, in or around the instrument.

Safety Information . 13
Unpacking and Installation
•This equipment is designed to be installed in accordance with the Canadian Electrical
Code (CEC) Part 1, CSA C22.1 and CSA C22.2 No. 0.
•Inspect the instrument for any signs of damage caused in transit. If any damage is
discovered, access the Troubleshooting Guide on the PerkinElmer website
http://www.perkinelmer.com/LambdaBioXLSSupport and follow the process there. If
you do not have access to the website, contact Customer Care (see
Appendix 2:
Customer Care Contacts
on page 87).
•Ensure your proposed installation site conforms to the environmental conditions for safe
operation:
Indoor use only.
Temperature range 5 °C to 35 °C.
Maximum relative humidity of 80% up to 31 °C, decreasing linearly to 50% at 40 °C.
NOTE: If you use the instrument in a room subjected to extremes of temperature change
during the day, it may be necessary to recalibrate (by switching off and then on
again) once thermal equilibrium has been established (2–3 hours).
•If the instrument has just been unpacked or has been stored in a cold environment, it
should be allowed to come to thermal equilibrium for 2–3 hours in the laboratory before
turning the instrument on. This will prevent calibration failure as a result of internal
condensation.
•The instrument must be placed on a stable, level bench or table that can take its weight
(approximately 4.5 kg) with enough space around the instrument so that air can
circulate freely.
•This equipment must be connected to the supply outlet with the power cord and power
supply provided. It can be used on 100–240 V, 50–60 Hz supplies. The power cord or
power supply outlet should be is fused at 3 A, 250 V.
•The instrument should be positioned so that the power cable can be readily
disconnected from the unit in the event of a hazard or malfunction occurring.
•Site the instrument in an atmosphere free from dust and corrosive fumes.
•Please read through the instrument User’s Guide supplied on the Lambda Bio/XLS
Documentation CD (L6050018) prior to use.
•Switch on the instrument via the ON/OFF button on the keypad after it has been
plugged in. The instrument will perform a series of self-diagnostic checks.
WARNING
If the equipment is used in a manner not specified herein, then th
e protection
provided by the equipment may be impaired and instrument warranty
withdrawn.

14 . Lambda XLS/XLS+ User's Guide

Installing your Instrument

16 . Lambda XLS/XLS+ User's Guide
Unpacking
CAUTION
Take great care when installing your instrument, and follow the
procedures described in this manual.
1. Check that you have the items listed in the following table.
Part Number Description
L7110189/L7110192 Lambda XLS/XLS+ (if applicable)
L7110190/L7110193 Lambda XLS/XLS+ with printer (if applicable)
L7110191/L7110194 Lambda XLS/XLS+ with SD (if applicable)
L6050014 Lambda Bio/XLS Safety Manual
L6050018 Lambda Bio/XLS Documentation CD
L7111025 Cuvette dust cover
L7110231 Lambda Bio/XLS Print Utility and Cable (if applicable)
L7110270 UV macro cuvettes (8 pack)
N/A Power cable
2. Examine the components for any signs of damage in shipment:
•Check the enclosure of the instrument for damage, and make sure that the
terminals are not damaged.
•Make sure that the compartment is free of dust or other foreign matter.
NOTE: In the event of damage, or missing parts, access the Troubleshooting Guide on the
PerkinElmer website at http://www.perkinelmer.com/LambdaBioXLSSupport and
follow the process there. If you do not have access to the website, contact Customer
Care (see Appendix 2: Customer Care Contacts on page 87).

Installing your Instrument . 17
Connecting to the Line Power Supply
WARNING
Electrical Hazard
To prevent potential injury to yourself and damage to the instrument,
make any electrical connections in the system before connecting to the
line power supply.
The instrument automatically adjusts to the correct operating voltage.
Connect to the supply outlet with power supply provided and the power cord that is
appropriate for your region.
You must use the power cord and power supply supplied with the instrument.
NOTE: If the instrument has just been unpacked or has been stored in a cold environment,
it should be allowed to come to thermal equilibrium in the laboratory for 2–3 hours
before turning the instrument on. This will prevent calibration failure as a result of
internal condensation.
Figure 1 Rear connections on the Lambda Bio/XLS instrument
The ON/OFF switch is located at the top left-hand of the keypad panel.
USB cable connection
Line power in

18 . Lambda XLS/XLS+ User's Guide

Using your Instrument

20 . Lambda XLS/XLS+ User's Guide
Overview of the Instrument
The Lambda XLS/XLS+ instrument is a simple-to-use UV/Visible spectrometer with a CCD
array detector (1024 pixels).
To access the folders and functions on your Lambda XLS/XLS+ instrument, navigate around
the display using the alphanumeric keys and the navigation arrows on the keypad.
Figure 2 Keypad and display
Key Action
Display panel Displays folders and menu options that guide you through taking
measurements and viewing your results.
Turns the instrument on or off.
Use the four arrows to navigate around the display and select the required
setting from the active (highlighted) option.
The Options key displays the options for that method or folder. See
Options
on page 25 for more information. Options unique to a specific method are
described in the relevant section.
The 0A/100%T key sets a reference solution measurement to 0.000 A or
100%T at the current wavelength. When in scan mode, it performs a
reference scan.
Arrow keys
Alphanumeric keys
Cancel/Back/Stop
Run/OK/Next/Scan
0A/100%T (scan reference)
Display panel
ON/OFF
View Options