SAGE Analytics TOX Detective User manual
Instruction Manual
Table of Contents
TOX Detective™Instructions .........................1-7
Limits Of Detection .................................8-9
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1
(20) Pipettes
(2) RO Water Vials
(10) Test Packets
(1) TOX Detective unit
TOX Detective Sampling Kit
(1) AC/USB Power Supply (1) Rugged Pelican™
Carrying Case
(10) Plastic ZiplockTM Bags
TOX Detective™
What’s Included
The ToxDetective is a fully integrated detection system capable of simul-
taneously testing cannabis samples for numerous pesticide compounds as well
as toxic/heavy metals in various aqueous matrices. Both raw cannabis ower &
trim can be tested using the included ToxDetective Sampling Kit.
The ToxDective is an advanced hand-held reader device that is used to analyze
the test packs through a simple, touch-screen operation that initiates an
automated data analysis and then stores an electronic record of the test results.
Accompanied by the ToxDetective Software, the ToxDetective reader allows the
user to download the labeled time and date stamped results to a PC or Cloud
database for analysis, review and archive. These features, along with its rugged
design, make the ToxDetective ideal for eld use.
INTENDED USE
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1Power On the Tox Detective
The ToxDetective detects the presence of acetylcholinesterase-inhibiting com-
pounds such as pesticides, nerve agents, toxic/heavy metals, and other toxicants
in/on cannabis. The ToxDetective has been designed to analyze test results
obtained using the ToxDetective a/b test packs. The reader measures the uo-
rescence generated on each test ticket pair (001a & 001b) and converts them to
a positive or negative result.
STORAGE/USAGE
For optimum results, store the ToxDetective a/b test packs at room temperature
(23ºC or 73ºF). The a/b test packs have a two (2) year shelf life. The expiration
date is printed on the foil pouch of each test pack.
• Do not use assays beyond their expiration date
• Do not use assays stored in a previously opened and/or damaged foil pouch
• Do not reuse assays.
• Avoid direct sunlight or extreme high/cold temperatures
Before starting a test, power on the ToxDetective reader
(power button located on right hand side of device).
Swipe the home screen with your nger to locate and
activate the application (The application is called ACE).
Once the application has loaded, it will
prompt you to engage the USB connection. Check the
box and press “OK”. The system is now ready for use.
IMPORTANT: Failure to check the USB box and press “OK”
when prompted will result in failure to read any test tickets.
TEST PRINCIPLE
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Running a Test
IMPORTANT: Each a/b test pack contains two test tickets. You MUST run
both tickets for a complete test because the tickets are sensitive to different
contaminants.. The A ticket is most sensitive to carbamates, the B ticket is most
sensitive to organophosphates.
1. Open the foil pouch and remove the test tickets. (001a ticket & 001b ticket
each per test packet). The test tickets are liquid-based lateral ow assays
(LFA), thus requiring the cannabis sample (raw ower or trim) to be “washed”
so that any pesticides/toxic compounds will be suspended in an aqueous
matrix prior to running on both test tickets in each a/b test pack.
2. Fill the Ziplock™ bag with 5 mL of reverse osmosis (RO) water and place
1/2 gram (0.5g) of cannabis into the bag. For best results, break up the
cannabis if in bud form and saturate thoroughly, by gently rubbing and
tilting the bag side to side to ensure the cannabis is completely mixed and
saturated for at least 1 minute.
3. Now, the water extract will be used to run the test. Both “A” wells of either
test ticket will receive this extract water, while both “B” wells will receive the
control (RO) water.
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4
4. “A” sample well: Using the
provided pipette*, transfer
the wash uid from the
sample bag and dispense
5-6 drops of the extract
water into the “A” well of both
001a and 001b test tickets.
6. Start the timers for both
001a & 001b test tickets
and allow each to develop
for 12 minutes. After 12
minutes, the reader will
automatically alarm to let
you know its time to read
the tickets.
*If you are testing trim or soil that contains large particulates, it is highly
recommended that you use the Trim & Soil Test Kit (sold separately -
Part # TST-001). The test ticket well can become clogged and not run
properly if these large particulates are not ltered out beforehand.
To use the Trim & Soil test kit:
1. Insert the syringe (no lter attached yet) into the wash uid from
the sample bag and pull up on the plunger to collect the wash uid.
2. Attach the round lter (luer lock side) to the tip of the syringe by
screwing it on clockwise and dispense the wash liquid into the
provided collection tube by fully pressing the syringe plunger
all the way down.
3. Run the test tickets as normal using the ltered liquid from the
collection tube for the “A” well of both 001a and 001b test tickets.
001b
001a
5. “B” control well: Using another
pipette (don’t use the same
pipette as you did to ll the
“A” sample well), transfer 5-6
drops of RO (control) water
to ll the control well of both
001a and 001b test tickets. 001b
001a
TST-001
5
While the a/b test tickets are developing for 12 minutes,
enzymes in the test tickets will interact with possible
pesticides or toxic metals that may be present
in your sample. If so, the enzyme will be
destroyed or inhibited, thus not able to
uoresce as strongly when compared
to the “B” control well.
7. After the 12 minutes is up, insert the
001a test ticket into the reader
8. Using the reader, press “Read Assay”.
A new screen will appear which will
allow you to enter a sample name.
Press “Read Assay” again to initiate
the automatic analysis of the ticket.
Results will be displayed on the
screen.
9. Label your sample (for both
001a & 001b test tickets) in
the tag window and press
“Read Assay” again to initiate
automatic analysis of the test
ticket.
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10. The ToxDetective reader will process the image of each test ticket,
comparing the uorescence of the “A” sample well with the “B” control well
and display a positive or negative result. Make sure to run both the 001a as
well as the 001b test tickets for a complete test of your sample.
Results: If both tickets are negative, the sample is negative / pass) -
no contaminants detected. If at least one ticket is positive, the sample is
positive / fail) - contaminates are detected.
Possible Interferences: Samples and sample extracts that are acidic, or
contain high levels of alcohol may interfere with the test . Testing dirt or
trim that contains large particulates can cause the test ticket to get clogged
and not run properly if these large particulates are not ltered out. This can
be easily solved with an additional syringe lter that is sold separately.
11. Each test result is uniquely recorded with a record number and date/
time stamp. The data can be reviewed on the reader for quick analysis or
exported to a PC computer for later use.
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DISCLAIMER
The ToxDetective Test Kit is an initial screening test and cannot be used to
certify cannabis products as safe or unsafe for consumption. When used
in strict accordance with instructions, the ToxDetective Test Kit provides
approximate results for one or more acetylcholinesterase inhibitors such
as organophosphate and carbamate pesticides, toxic metals, or naturally
occurring acetylcholinesterase inhibitors.
Testing for Myclobutinil (a.k.a Eagle 20)
A separate test ticket is available for purchase (Part #1CA-002-PT)
if you wish to be able to test for this particular trizole based fungicide.
You can order on line at www.toxdetective.com
ToxDetective Export Instructions
1. From the ASDR home screen, select General Settings and then select
EXPORT. Note: YOU MUST CLICK EXPORT PRIOR TO RETRIEVING DATA
FROM READER. By clicking “Export” a data le is created in the unit
memory which is then selectable once you connect to PC. Note:
You can only export data to a PC, it does not work with MAC.
2. Using the USB power cord, connect the USB plug to a windows PC and
then connect the micro USB to the Tox Detective charging port. If this
is the rst time connecting to a PC, you will be notied by the PC that
it is setting up the “storage device” (it might call it something else, but
basically it lets you know, similar to a USB drive that it is setting up the
drivers/etc.)
It may ask you to “allow ACE to access the USB device”– check the box
and press OK. The Tox Detective should automatically initialize.
3. Access the Tox Detective data by locating the Tox Detective unit in your
windows le explorer
4. Select the Images and Excel Export le in the “ACEImage” folder from
above and analyze your data.
5. The excel le will correlate to the specic image shown above by
chronological order so it is important to make sure the date/time is
accurate to nd the images and related data you want. If the date/time is
wrong, the record #/name of the testing sample can be used to match up.
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LIMITS OF DETECTION
COMPOUND Interpreted Sensitivity Action Level (ug/g) “PPM”
Category I Residual Pesticides (California) Inhalable Cannabis Products Other Cannabis Products
Aldicarb I No Detection Allowed No Detection Allowed
Carbofuran I No Detection Allowed No Detection Allowed
Chlordane S No Detection Allowed No Detection Allowed
Chlorfenapyr S No Detection Allowed No Detection Allowed
Chlorpyrifos I No Detection Allowed No Detection Allowed
Coumaphos I No Detection Allowed No Detection Allowed
Daminozide S No Detection Allowed No Detection Allowed
DDVP (Dichlorvos) I No Detection Allowed No Detection Allowed
Dimethoate I No Detection Allowed No Detection Allowed
Ethoprophos S No Detection Allowed No Detection Allowed
Etofenprox S No Detection Allowed No Detection Allowed
Fenoxycarb S No Detection Allowed No Detection Allowed
Fipronil S No Detection Allowed No Detection Allowed
Imazalil S No Detection Allowed No Detection Allowed
Methiocarb E No Detection Allowed No Detection Allowed
Methyl parathion I No Detection Allowed No Detection Allowed
Mevinphos (Phosdrin) I No Detection Allowed No Detection Allowed
Paclobutrazol No Detection Allowed No Detection Allowed
Propoxur I No Detection Allowed No Detection Allowed
Spiroxamine No Detection Allowed No Detection Allowed
Thiacloprid S No Detection Allowed No Detection Allowed
Category II Residual Pesticides (California) Inhalable Cannabis Products Other Cannabis Products
Abamectin S 0.1 0.3
Acephate E 0.1 5
Acequinocyl S 0.1 4
Acetamiprid S 0.1 5
Azoxystrobin S 0.1 40
Bifenazate S 0.1 5
Bifenthrin 3 0.5
Boscalid S 0.1 10
Captan S 0.7 5
Carbaryl S 0.5 0.5
Chlorantraniliprole S 10 40
Clofentezine 0.1 0.5
B-Cyuthrin S 2 1
Cypermethrin 1 1
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Key
I = sensitivity at or below inhalable product limits / sub ppm or single digit ppm if not listed
E = sensitivity at or below other (edible) product limits / 10-40 ppm if not listed
S = sensitivity to pesticide but not at current resolutions required by CA
Blank = not able to be detect that particular pesticide at any sensitivity / level
COMPOUND Interpreted Sensitivity Action Level (ug/g) “PPM”
Category II Residual Pesticides (Calif.) Continued Inhalable Cannabis Products Other Cannabis Products
Diazinon S 0.1 0.2
Dimethomorph S 2 20
Etoxazole 0.1 1.5
Fenhexamid S 0.1 10
Fenpyroximate S 0.1 2
Flonicamid S 0.1 2
Fludioxonil S 0.1 30
Hexythiazox 0.1 2
Imidacloprid S 5 3
Kresoxim-methyl S 0.1 1
Malathion E 0.5 5
Metalaxyl S 2 15
Methomyl I 1 0.1
Myclobutanil S 0.1 9
Naled (Dibrom 1,2-Dibromo) I 0.1 0.5
Oxamyl I 0.5 0.2
Pentachloronitrobenzene 0.1 0.2
Permethrin 0.5 20
Phosmet S 0.1 0.2
Piperonyl butoxide 3 8
Prallethrin 0.1 0.4
Propiconazole S 0.1 20
Pyrethrins 0.5 1
Pyridaben 0.1 3
Spinetoram 0.1 3
Spinosad 0.1 3
Spiromesifen 0.1 12
Spirotetramat 0.1 13
Tebuconazole 0.1 2
Thiamethoxam 5 4.5
Trioxystrobin 0.1 30
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