Sartorius ePET User manual

User Manual
ePET® Electronic Pipette

English 1
Chinese 23
ePET® Electronic Pipette

iii
English 1
Chinese 23
ePET® Electronic Pipette

1
1
Contents
1. Intended Use .....................................................................................................2
2. Product Description .......................................................................................2
2.1 ePET® Single-Channel Pipettes...................................................2
2.2 ePET® Multichannel Pipettes .......................................................3
3. Pipette Tips ........................................................................................................3
4. Unpacking ..........................................................................................................3
4.1 Electrical Specifications ................................................................ 3
4.2 Charging the Pipette ......................................................................4
5. Pipette Parts and Materials .........................................................................5
6. Pipette Description .........................................................................................6
6.1 Controls ...............................................................................................6
6.2 Start Button ....................................................................................... 6
6.3 Direction Symbols ............................................................................ 6
6.4 Display ..................................................................................................7
6.5 Loading and Ejecting Tips .............................................................7
6.6 Optional Filters..................................................................................7
7. Programming the Pipette ............................................................................8
7.1 Mode Selection and Mode Recall .............................................. 8
7.2 Setting Speeds ..................................................................................8
7.3 Pipetting Mode (P) ..........................................................................9
7.4 Multi-Dispensing Mode (d).........................................................10
7.5 Diluting Mode (dd) ........................................................................11
7.6 Mixing Mode with Pipetting or Diluting ...............................12
8. Pipetting Recommendations ....................................................................13
8.1 Dispensing with Blow-out ..........................................................13
8.2 Dispensing without Blow-out ...................................................13
8.3 Other Recommendations ............................................................14
9. Storage ..............................................................................................................14
10. Performance Testing ....................................................................................15
10.1 Testing the Performance .............................................................15
11. Maintenance ...................................................................................................17
11.1. Cleaning and Decontaminating the Pipette ........................17
11.2 In-house Maintenance .................................................................17
11. 3 Battery Replacement ....................................................................18
12. Troubleshooting .............................................................................................19
13. Warranty Information .................................................................................21
14. Recycling Instructions (WEEE) .................................................................21
15. Performance Specifications ......................................................................22

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2
1. Intended Use
The ePET® pipette is intended to be used in liquid
handling applications for dispensing liquids in
the volume range of 0,2 μl to 5 ml. With ePET®
pipettes it is recommended to use Sartorius
Optifit Tips or SafetySpace™ Filter Tips to ensure
the best compatibility and performance.
This liquid handling device is designed and
manufactured to be used as general purpose
laboratory equipment. Before operating, it is
recommended to read this user manual, which
contains useful information, for example, for
good pipetting practice.
2. Product Description
Your new Sartorius ePET® electronic pipette
offers a cost-effective electronic pipetting
alternative by using a direct charging system,
which requires no charging stand. The self-
calibrating, microprocessor-based system
reduces the possibility for human error and
instrument contamination, by controlling all
piston movements. Also, some models offer
replaceable Safe-Cone Filters to help prevent
contamination and damage.
Its light weight and ergonomic controls take the
effort out of pipetting to help reduce the risk
of repetitive strain injuries (RSI) that commonly
occur in manual pipetting. All ePET® electronic
pipettes operate on the air-displacement principle
and use disposable tips.
2.1 ePET® Single-Channel Pipettes
Order
No.
Volume Range
µl
Increment
µl
Tip µl
710521ET 0.2 - 10 0.1 10
710011ET 5 - 100 1 200 , 350
710041ET 50 - 1200 5 1200
710501ET 100 - 5000 50 5000
All pipettes include universal AC-adaptor (EU, UK, US/JPN,
AUS, KOR and CHN plugs).

3
2
1. Intended Use
The ePET® pipette is intended to be used in liquid
handling applications for dispensing liquids in
the volume range of 0,2 μl to 5 ml. With ePET®
pipettes it is recommended to use Sartorius
Optifit Tips or SafetySpace™ Filter Tips to ensure
the best compatibility and performance.
This liquid handling device is designed and
manufactured to be used as general purpose
laboratory equipment. Before operating, it is
recommended to read this user manual, which
contains useful information, for example, for
good pipetting practice.
2. Product Description
Your new Sartorius ePET® electronic pipette
offers a cost-effective electronic pipetting
alternative by using a direct charging system,
which requires no charging stand. The self-
calibrating, microprocessor-based system
reduces the possibility for human error and
instrument contamination, by controlling all
piston movements. Also, some models offer
replaceable Safe-Cone Filters to help prevent
contamination and damage.
Its light weight and ergonomic controls take the
effort out of pipetting to help reduce the risk
of repetitive strain injuries (RSI) that commonly
occur in manual pipetting. All ePET® electronic
pipettes operate on the air-displacement principle
and use disposable tips.
2.1 ePET® Single-Channel Pipettes
Order
No.
Volume Range
µl
Increment
µl
Tip µl
710521ET 0.2 - 10 0.1 10
710011ET 5 - 100 1 200 , 350
710041ET 50 - 1200 5 1200
710501ET 100 - 5000 50 5000
All pipettes include universal AC-adaptor (EU, UK, US/JPN,
AUS, KOR and CHN plugs).
3
2.2 ePET® Multichannel Pipettes
Order No. Volume Range µl Increment µl Tip µl
710201ET 8-Ch 0.2 - 10 0.1 10
710211ET 8-Ch 5 - 100 1 200 , 350
710221ET 8-Ch 25 - 250 5 350
710801ET 8-Ch 50 - 1200 10 1200
3. Pipette Tips
It is recommended that Sartorius Optifit Tips
or SafetySpace™ Filter Tips are used with
Sartorius pipettes. This ensures the best possible
compatibility and thus best possible accuracy
and precision, as these tips are designed for the
pipette in question.
Optifit Tips and SafetySpace™ Filter Tips are also
available as Low Retention Tips.
4. Unpacking
The ePET® pipette package contains the following
items:
1. Pipette
2. Grease
3. AC/DC Adapter (acts as a recharging unit)
4. Instructions for use
5. Filters and tweezers (pipettes > 10 µl)
6. ISO8655-6 compliant performance certificate
Make sure that all items are included and that no
damage has occurred during shipping.
NOTE! The pipette can only be charged with the
original AC/DC Adapter supplied with the pipette.
4.1 Electrical Specifications
Battery
- Rechargeable NiMH battery
- Charging time a maximum of 12 hours for an
empty battery
AC/DC Adaptor
- Input voltage and main plug according to
local requirements
- Output voltage 9 VDC

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4
4.2. Charging the Pipette
An ON/OFF switch is located at the top of the
pipette. This switch protects the battery from
discharging while the pipette is not in use and
not connected to the charger.
1. Switch the pipette ON (the far left position).
2. Connect the charger to a compatible AC
outlet and to the pipette's charger port.
NOTE! The charging light will remain illuminated,
when properly connected to the AC/DC
charger.
3. If the pipette is new, or the battery charge
is low, keep the pipette connected to the
charging unit for 12 hours to fully charge the
battery before continuing use.
4. Display shows E Press the START button
twice and the pipette is ready for the default
pipetting at maximum volume or for program
changes.
NOTE! The pipette will charge in both ON and OFF
positions. Leaving the pipette switched ON while
charging will retain user settings.
Switching it OFF will reset the unit to default
settings.
NOTE! If the pipette is left ON for several days,
the battery will discharge. As a result the display
will turn off, and there will be no response from
the keyboard or START button. This is because the
charge of the battery is below operating level.
Switch ON the
pipette; the power
indicator light
comes on.
Connect the
charger to the
pipette's charger
port

5
4
4.2. Charging the Pipette
An ON/OFF switch is located at the top of the
pipette. This switch protects the battery from
discharging while the pipette is not in use and
not connected to the charger.
1. Switch the pipette ON (the far left position).
2. Connect the charger to a compatible AC
outlet and to the pipette's charger port.
NOTE! The charging light will remain illuminated,
when properly connected to the AC/DC
charger.
3. If the pipette is new, or the battery charge
is low, keep the pipette connected to the
charging unit for 12 hours to fully charge the
battery before continuing use.
4. Display shows E Press the START button
twice and the pipette is ready for the default
pipetting at maximum volume or for program
changes.
NOTE! The pipette will charge in both ON and OFF
positions. Leaving the pipette switched ON while
charging will retain user settings.
Switching it OFF will reset the unit to default
settings.
NOTE! If the pipette is left ON for several days,
the battery will discharge. As a result the display
will turn off, and there will be no response from
the keyboard or START button. This is because the
charge of the battery is below operating level.
Switch ON the
pipette; the power
indicator light
comes on.
Connect the
charger to the
pipette's charger
port
5
Charger port
cover (PC)
Display
(glass)
Finger support
(at the back)
(ASA/PC)
Start
button
(POM)
Handle
(ASA/PC)
Tip ejector
lever (POM)
Tip ejector
collar (PP)
Tip cone
10 μl (PVC)
100 μl (SS)
1200 μl (PPS)
5000 μl (PE)
Keyboard
(silicon keys)
Tip cone
housing
(ASA/PC)
Display
(glass)
Tip ejector
lever (POM)
Tip ejector bar
(PETP)
Start button
(POM)
Handle
(ASA/PC)
Keyboard
(silicon keys)
Tip cones
10-250 μl (sealing: TPU)
10-250 μl (PVDF)
50-1200 μl (PPS)
5. Pipette Parts and Materials
ePET® Multichannel
ePET® Single-Channel
Power ON/OFF
switch

6
6
Start Button
Tip Ejector Lever
Control Keys


M

E
S
Battery
Compartment
Power Switch
Charger
Port
6. Pipette Description
The control and the programming of the ePET®
electronic pipette are performed using the
control keys and display.
6.1. Controls
- Power Switch
- Charger Connection
- Battery Compartment
Control Keys:
S Changes speed
M Moves to next mode
 Increases volume or speed
 Decreases volume or speed
E Confirms setting changes
 Switches Mixing on / off
Start Button
- Triggers all mechanical operations
- Colour-coded for easy identification of
pipette volume
Tip Ejector Lever
- Squeeze to remove pipette tip(s)
6.2. Start Button
The START button triggers the aspiration and
dispensing operations according to the selected
operating mode. Only a quick click is required to
operate the button. If the START button is held
down, the piston will stop in the lowest position
until the button is released. This feature is
applicable to all modes of operation, except for
multiple dispensing (d).
6.3. Direction Symbols
These symbols indicate the direction in which the
piston moves upon pressing the START button.
The small RIGHT ARROW in the display means
that the next function is to aspirate the liquid.
The small LEFT ARROW, in turn, indicates the
dispensing function in accordance to the selected
operating mode.

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7
6.4. Display
The left side of the display is the status indicator.
It informs the user about parameters to be
programmed, functions to be performed and the
number of dispensings available.
The right side of the display is used for
programming and displaying the various volumes
needed in different operating modes.
6.5. Loading and Ejecting Tips
Original Sartorius tips are recommended for use
with Sartorius ePET® pipettes. Before fitting a tip
make sure that the pipette tip cone is clean. Press
the tip onto the cone of the pipette firmly, to
ensure an airtight seal. The tip is tightly loaded,
when a visible sealing-ring has formed between
the tip and the tip cone.
Sartorius ePET® pipettes are designed for simple
and light attachment and ejection of the tips. To
eject the tip, simply place the pipette over the
waste container and squeeze the tip ejector lever.
6.6. Optional Filters
The tip cones of some ePET® electronic pipettes
allow the use of a removable Safe-Cone Filter as
an option. The filter prevents liquids and vapours
from entering the pipette. The filter does not
affect the calibration of the pipette. Sartorius
filter-tweezers should be used to insert or remove
a Safe-Cone Filter to avoid contamination
through contact with it.
Order Information
Safe-Cone Filter
Order No. Channels Volume Range µl Increment µl Standard Plus
710521ET 10.2 - 10 0.1 N/A N/A
710011ET 15 - 100 1 N/A N/A
710041ET 150 - 1200 5 721006 721016
710501ET 1100 - 5000 50 721006 721016
710201ET 80.2 - 10 0.1 N/A N/A
710211ET 85 - 100 1721014 N/A
710221ET 825 - 250 5 721014 N/A
710801ET 850 - 1200 10 721006 721016
The use of tweezers
to insert or remove
a Safe-Cone Filter
Squeeze the tip
ejector lever, to
eject the tip
Visible seal when tip
is loaded tightly
6
Start Button
Tip Ejector Lever
Control Keys


M

E
S
Battery
Compartment
Power Switch
Charger
Port
6. Pipette Description
The control and the programming of the ePET®
electronic pipette are performed using the
control keys and display.
6.1. Controls
- Power Switch
- Charger Connection
- Battery Compartment
Control Keys:
S Changes speed
M Moves to next mode
 Increases volume or speed
 Decreases volume or speed
E Confirms setting changes
 Switches Mixing on / off
Start Button
- Triggers all mechanical operations
- Colour-coded for easy identification of
pipette volume
Tip Ejector Lever
- Squeeze to remove pipette tip(s)
6.2. Start Button
The START button triggers the aspiration and
dispensing operations according to the selected
operating mode. Only a quick click is required to
operate the button. If the START button is held
down, the piston will stop in the lowest position
until the button is released. This feature is
applicable to all modes of operation, except for
multiple dispensing (d).
6.3. Direction Symbols
These symbols indicate the direction in which the
piston moves upon pressing the START button.
The small RIGHT ARROW in the display means
that the next function is to aspirate the liquid.
The small LEFT ARROW, in turn, indicates the
dispensing function in accordance to the selected
operating mode.

8
8


M

E
S
7. Programming the
Pipette
Programming is performed using the six-key
keyboard and the LC-display. There are three
operating modes offering special functions and
varying speeds to choose from.
7.1. Mode Selection and Mode
Recall
1. Press M repeatedly to view the available
modes of the pipette.
2. Press E when the desired mode is
displayed. The pipette is ready for use in the
mode selected.
NOTE: The mode can only be activated when the
piston is in its home position (arrow-right is lit),
and not during an aspiration or dispensing cycle.
7.2. Setting Speeds
1. Press S to display the current aspiration
speed.
2. Press  or  until desired aspiration
speed is displayed (”5” Fast and ”1” Slow).
3. Press E to confirm selected speed. Display
shows the current dispense speed.
4. Press  or  until desired dispense speed
is displayed (”5” being Fast and ”1” Slow).
5. Press E to confirm speed selection.
NOTE: The speed may not be changed during
aspiration or dispensing cycles.
NOTE: The default speed is 3 for all speed
settings.

9
8


M

E
S
7. Programming the
Pipette
Programming is performed using the six-key
keyboard and the LC-display. There are three
operating modes offering special functions and
varying speeds to choose from.
7.1. Mode Selection and Mode
Recall
1. Press M repeatedly to view the available
modes of the pipette.
2. Press E when the desired mode is
displayed. The pipette is ready for use in the
mode selected.
NOTE: The mode can only be activated when the
piston is in its home position (arrow-right is lit),
and not during an aspiration or dispensing cycle.
7.2. Setting Speeds
1. Press S to display the current aspiration
speed.
2. Press  or  until desired aspiration
speed is displayed (”5” Fast and ”1” Slow).
3. Press E to confirm selected speed. Display
shows the current dispense speed.
4. Press  or  until desired dispense speed
is displayed (”5” being Fast and ”1” Slow).
5. Press E to confirm speed selection.
NOTE: The speed may not be changed during
aspiration or dispensing cycles.
NOTE: The default speed is 3 for all speed
settings.
9
↑
↑
7.3. Pipetting Mode (P)
The pipette performs ordinary pipetting with
blow-out.
1. Press M to display .
2. Press E to confirm the mode change.
3. Select the desired pipetting volume by using
 to increase, and  to decrease.
NOTE: While holding down  or , the volume
display starts to scroll faster.
4. Press E to confirm selection.
5. Position the tip to aspirate and press the
START button.
6. Position the tip to dispense. Press the START
button. The tip is emptied with a blow-out
and is ready for the next pipetting.
Step 5 Aspiration
(Pipetting Mode)
Step 6 Dispensing
(Pipetting Mode)

10
10
↑
7.4. Multi-Dispensing Mode (d)
In the Multi-Dispensing Mode (d), the pipette
performs repetitive dispensings of a selected
volume. During this operation, the desired volume,
plus the automatically selected excess volume, is
aspirated into the tip.
1. Press M to display .
2. Press E to confirm the mode change.
3. Press  or  until the multi-dispense
volume is displayed.
4. Press E to confirm selection.
5. Press  or  until the desired number of
aliquots is displayed.
6. Press E to confirm selection.
7. Position the tip to aspirate and press the
START button. The * sign and ARROW LEFT
are lit to indicate the reset function.
8. Position the tip to discard priming excess and
press the START button.
9. To dispense, position the tip, press the START
button and repeat until the cycle is complete.
10. Finally, position the tip to discard any
remaining excess and press the START button
twice.
NOTE: Upon delivering the programmed volume,
it is also possible to continue to aspirate and
dispense the same volume without emptying in
between. To continue, keep the START button
pressed down and within one second the direction
of the arrow will change. Keeping the button
down, place the tip into the liquid again and the
sample is aspirated into the tip by releasing the
START button.
↑
↑
↑
Step 7 Aspiration
(Multi-Dispensing
Mode)
Step 8 Discarding
priming excess
(Multi-Dispensing
Mode)
Step 9 Dispensing
repeatedly (Multi-
Dispensing Mode)
Step 10 Discarding
excess (Multi-
Dispensing Mode)

11
10
↑
7.4. Multi-Dispensing Mode (d)
In the Multi-Dispensing Mode (d), the pipette
performs repetitive dispensings of a selected
volume. During this operation, the desired volume,
plus the automatically selected excess volume, is
aspirated into the tip.
1. Press M to display .
2. Press E to confirm the mode change.
3. Press  or  until the multi-dispense
volume is displayed.
4. Press E to confirm selection.
5. Press  or  until the desired number of
aliquots is displayed.
6. Press E to confirm selection.
7. Position the tip to aspirate and press the
START button. The * sign and ARROW LEFT
are lit to indicate the reset function.
8. Position the tip to discard priming excess and
press the START button.
9. To dispense, position the tip, press the START
button and repeat until the cycle is complete.
10. Finally, position the tip to discard any
remaining excess and press the START button
twice.
NOTE: Upon delivering the programmed volume,
it is also possible to continue to aspirate and
dispense the same volume without emptying in
between. To continue, keep the START button
pressed down and within one second the direction
of the arrow will change. Keeping the button
down, place the tip into the liquid again and the
sample is aspirated into the tip by releasing the
START button.
↑
↑
↑
Step 7 Aspiration
(Multi-Dispensing
Mode)
Step 8 Discarding
priming excess
(Multi-Dispensing
Mode)
Step 9 Dispensing
repeatedly (Multi-
Dispensing Mode)
Step 10 Discarding
excess (Multi-
Dispensing Mode)
11
↑
↑
↑
↑
7.5. Diluting Mode (dd)
In the Diluting Mode (dd), two different solutions
separated by an air gap are aspirated and then
dispensed together with an automatic blow-
out. The purpose of the air gap is to prevent
contamination while aspirating the second
volume, but it will not prevent the two liquids
from mixing in the tip.
1. Press M to display .
2. Confirm the mode change by pressing E.
3. Select the desired diluent volume (volume 1)
by pressing  or .
4. Confirm by pressing E.
5. Press  or  to select the sample volume
(volume 2) on the display.
6. Press E to confirm selection.
7. Position the tip to aspirate volume 1 and
then press the START button.
8. With the tip in the air, press the START
button again to aspirate air to form an air
gap.
9. Position the tip to aspirate volume 2 and
press the START button.
10. Finally, position the tip to dispense, and press
the START button.
Step 7 Aspiration
(Diluting Mode)
Step 8 Air aspiration
for air-gap
(Diluting Mode)
Step 9 Aspiration of
2nd fluid
(Diluting Mode)
Step 10 Dispensing
(Diluting Mode)

12
13
To Mix:
1. Position the tip in the solution, then press
and hold the START button. The mixing is
done automatically as long as the START
button is held down.
2. Position the tip to dispense. Press the START
button twice.
NOTE: The mixing is done with about 70% of the
total volume.
8. Pipetting
Recommendations
By using the various operating modes and special
functions, several different liquid handling
procedures are possible. Modes P and dd
feature an automatic blow-out and others
leave the excess liquid in the tip. Follow the
recommendations below to ensure optimal
performance.
8.1. Dispensing with Blow-out
The P and dd modes have an automatic blow-out
function, followed by an immediate return of the
piston to the ”home” position. To avoid accidental
aspiration of the liquid back into the tip, it is
recommended that the dispensing is always done
above the surface of the liquid.
By holding the START button down, during
dispensing, the piston will stop in the lowest
position. This allows the tip to be placed against
the bottom or the wall of the container. Once
the liquid is dispensed, the tip can be removed
from the container and the START button can be
released.
8.2. Dispensing without Blow-out
The pipette will not perform the blow-out
function while using the d mode. Therefore,
it is recommended that dispensing is always
performed with the tip set against the wall or the
bottom of the container. The use of the d mode
is especially useful when pipetting small volumes
or solutions that have a tendency to foam, or
have a high viscosity.
↑
↕
Step 1 Mixing
(Mixing Mode)
Step 2 Dispensing
(Mixing Mode)
12
7.6. Mixing Mode with Pipetting or
Diluting (*)
In the Mixing Mode, the piston is automatically
moved up and down to mix the liquid in the
delivery vessel. The mixing time is controlled by
the START button.
1. Use M to select either or on the
display.
2. Press  to switch on mixing. The display
should show either or .
NOTE: Pressing  will alternately switch mixing
on and off.
3. Confirm the mode change by pressing E.
For Pipetting:
1. Press  to increase, and  to decrease.
2. Press E to confirm selection.
3. Position the tip to aspirate and press START
button.
4. Position to dispense and press the START
button.
For Diluting:
1. Select the desired diluent volume (volume 1)
by using  or .
2. Confirm selection by pressing E.
3. Press  or  to select the sample volume
(volume 2) .
4. Confirm selection by pressing E.
5. Position the tip to aspirate volume 1, and
press the START button.
6. With the tip in the air, press the START
button to aspirate air for an air gap.
7. Position the tip to aspirate volume 2 and
press the START button.
8. Finally, position the tip to dispense and press
the START button.

13
13
To Mix:
1. Position the tip in the solution, then press
and hold the START button. The mixing is
done automatically as long as the START
button is held down.
2. Position the tip to dispense. Press the START
button twice.
NOTE: The mixing is done with about 70% of the
total volume.
8. Pipetting
Recommendations
By using the various operating modes and special
functions, several different liquid handling
procedures are possible. Modes P and dd
feature an automatic blow-out and others
leave the excess liquid in the tip. Follow the
recommendations below to ensure optimal
performance.
8.1. Dispensing with Blow-out
The P and dd modes have an automatic blow-out
function, followed by an immediate return of the
piston to the ”home” position. To avoid accidental
aspiration of the liquid back into the tip, it is
recommended that the dispensing is always done
above the surface of the liquid.
By holding the START button down, during
dispensing, the piston will stop in the lowest
position. This allows the tip to be placed against
the bottom or the wall of the container. Once
the liquid is dispensed, the tip can be removed
from the container and the START button can be
released.
8.2. Dispensing without Blow-out
The pipette will not perform the blow-out
function while using the d mode. Therefore,
it is recommended that dispensing is always
performed with the tip set against the wall or the
bottom of the container. The use of the d mode
is especially useful when pipetting small volumes
or solutions that have a tendency to foam, or
have a high viscosity.
↑
↕
Step 1 Mixing
(Mixing Mode)
Step 2 Dispensing
(Mixing Mode)
12
7.6. Mixing Mode with Pipetting or
Diluting (*)
In the Mixing Mode, the piston is automatically
moved up and down to mix the liquid in the
delivery vessel. The mixing time is controlled by
the START button.
1. Use M to select either or on the
display.
2. Press  to switch on mixing. The display
should show either or .
NOTE: Pressing  will alternately switch mixing
on and off.
3. Confirm the mode change by pressing E.
For Pipetting:
1. Press  to increase, and  to decrease.
2. Press E to confirm selection.
3. Position the tip to aspirate and press START
button.
4. Position to dispense and press the START
button.
For Diluting:
1. Select the desired diluent volume (volume 1)
by using  or .
2. Confirm selection by pressing E.
3. Press  or  to select the sample volume
(volume 2) .
4. Confirm selection by pressing E.
5. Position the tip to aspirate volume 1, and
press the START button.
6. With the tip in the air, press the START
button to aspirate air for an air gap.
7. Position the tip to aspirate volume 2 and
press the START button.
8. Finally, position the tip to dispense and press
the START button.

14
14
8.3. Other Recommendations
- Hold the pipette vertically and place the
tip a few millimeters into the liquid when
aspirating.
- Pre-rinse the tip by filling and emptying
the tip five times. This is important when
dispensing liquids which have a viscosity and
density different from water or a temperature
other than ambient.
- Check that the pipette, tip and liquid are at
room temperature.
- Avoid contaminating the tip cone.
- Connect the pipette charger when the pipette
is not in use. Switch the pipette OFF when not
in use, if it is not connected to the charging
unit.
- Never strike the tip cone against a tip tray
when mounting tips, as this can damage
several internal components.
- Do not drop the pipette or AC/DC-adaptor, as
this may cause excessive shock.
- Avoid exposing the unit to extreme
temperature changes, humidity and dust.
- Avoid rough handling. Moderate pressure is
all that is required when using the keyboard
or START-button.
- Avoid leaving the pipette laying on its side
with liquid in the tip. The liquid might leak
into the pipette's mechanism.
- Always dispense against the inside wall of the
receiving vessel. Remove the tip by drawing it
up along the inside wall.
- Change the filter on the tip cone regularly
(after 50 - 250 pipettings).
9. Storage
When not in use, it is recommended that the
pipette is stored on a Sartorius Linear stand,
dna noitisop NO eht ni ettepip ®TEPe eht htiw
connected to the charger. The green charging
light should be lit.
Order No. Item
725620Linear Stand for all Sartorius pipettes
Linear Stand
15
10. Performance Testing
It is recommended, the performance of your
ePET® pipettes be checked regularly (e.g. every 3
months), and always after in-house maintenance.
However, the user should establish a regular
testing routine for their pipettes with regard
to accuracy requirements of the application,
frequency of use, number of operators using
the pipette, nature of the liquid dispensed and
the acceptable maximum permissible errors
established by the user. (ISO 8655-1.)
10.1. Testing the performance
Performance testing should take place in a
draught-free room between 15° and 30°C, kept
constant within +/- 0.50°C, and with the relative
humidity above 50%. The pipette, tips and the
test water should have stood in the test room for
a sufficient length of time (at least 2 hours) to
reach equilibrium with the room's conditions. Use
distilled or de-ionised water (grade 3, ISO 3696).
Use an analytical balance with a readability of
0.01 mgs. (ISO 8655-6.)
Weighing
1. Adjust the desired test volume Vs.
2. Carefully fit the tip onto the tip cone.
3. Fill the tip with test water and expel it as waste
five times to achieve humidity equilibrium in
the dead air volume.
4. Replace the tip. Pre-wet the tip by filling it
once with test water and expel it as waste.
5. Aspirate the test water, immersing the tip only
2-3 mm below the surface of the water. Keep
the pipette vertical.
6. Withdraw the pipette vertically and touch the
tip against the inside wall of the test water
container.
7. Pipette the water into the weighing vessel,
touching the tip against the inside wall of
the vessel just above the liquid surface at an
angle of 30° to 45°. Withdraw the pipette by
drawing the tip 8-10 mm along the inner wall
of the weighing vessel.
8. Read the weight in mgs (mi).
9. Repeat the test cycle until 10 measurements
have been recorded.
10. Convert the recorded masses (mi) to volumes
(Vi)
Vi = mi * Z Z = correction factor
(Table follows)

15
14
8.3. Other Recommendations
- Hold the pipette vertically and place the
tip a few millimeters into the liquid when
aspirating.
- Pre-rinse the tip by filling and emptying
the tip five times. This is important when
dispensing liquids which have a viscosity and
density different from water or a temperature
other than ambient.
- Check that the pipette, tip and liquid are at
room temperature.
- Avoid contaminating the tip cone.
- Connect the pipette charger when the pipette
is not in use. Switch the pipette OFF when not
in use, if it is not connected to the charging
unit.
- Never strike the tip cone against a tip tray
when mounting tips, as this can damage
several internal components.
- Do not drop the pipette or AC/DC-adaptor, as
this may cause excessive shock.
- Avoid exposing the unit to extreme
temperature changes, humidity and dust.
- Avoid rough handling. Moderate pressure is
all that is required when using the keyboard
or START-button.
- Avoid leaving the pipette laying on its side
with liquid in the tip. The liquid might leak
into the pipette's mechanism.
- Always dispense against the inside wall of the
receiving vessel. Remove the tip by drawing it
up along the inside wall.
- Change the filter on the tip cone regularly
(after 50 - 250 pipettings).
9. Storage
When not in use, it is recommended that the
pipette is stored on a Sartorius Linear stand,
dna noitisop NO eht ni ettepip ®TEPe eht htiw
connected to the charger. The green charging
light should be lit.
Order No. Item
725620Linear Stand for all Sartorius pipettes
Linear Stand
15
10. Performance Testing
It is recommended, the performance of your
ePET® pipettes be checked regularly (e.g. every 3
months), and always after in-house maintenance.
However, the user should establish a regular
testing routine for their pipettes with regard
to accuracy requirements of the application,
frequency of use, number of operators using
the pipette, nature of the liquid dispensed and
the acceptable maximum permissible errors
established by the user. (ISO 8655-1.)
10.1. Testing the performance
Performance testing should take place in a
draught-free room between 15° and 30°C, kept
constant within +/- 0.50°C, and with the relative
humidity above 50%. The pipette, tips and the
test water should have stood in the test room for
a sufficient length of time (at least 2 hours) to
reach equilibrium with the room's conditions. Use
distilled or de-ionised water (grade 3, ISO 3696).
Use an analytical balance with a readability of
0.01 mgs. (ISO 8655-6.)
Weighing
1. Adjust the desired test volume Vs.
2. Carefully fit the tip onto the tip cone.
3. Fill the tip with test water and expel it as waste
five times to achieve humidity equilibrium in
the dead air volume.
4. Replace the tip. Pre-wet the tip by filling it
once with test water and expel it as waste.
5. Aspirate the test water, immersing the tip only
2-3 mm below the surface of the water. Keep
the pipette vertical.
6. Withdraw the pipette vertically and touch the
tip against the inside wall of the test water
container.
7. Pipette the water into the weighing vessel,
touching the tip against the inside wall of
the vessel just above the liquid surface at an
angle of 30° to 45°. Withdraw the pipette by
drawing the tip 8-10 mm along the inner wall
of the weighing vessel.
8. Read the weight in mgs (mi).
9. Repeat the test cycle until 10 measurements
have been recorded.
10. Convert the recorded masses (mi) to volumes
(Vi)
Vi = mi * Z Z = correction factor
(Table follows)

16
16
Z-values (μl/mg):
Temp. (°C) Air Pressure (kPa)
95 100 101.3 105
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
1.0028
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0028
1.0029
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0035
1.0036
1.0029
1.0030
1.0031
1.0032
1.0033
1.0034
1.0036
1.0037
11. Calculate the mean volume (⊽) delivered:
⊽ = (∑Vi)/10
12. To evaluate conformity, calculate the
systematic error es of the measurement:
in μl: es = ⊽ - Vs Vs = selected test volume
or in %: es = 100 (⊽-Vs)/Vs
13. To evaluate conformity, calculate the random
error of the measurement:
as standard deviation s = ∑Σ(Vi - ⊽)2
n - 1
n = number of measurements (10)
or as a coefficient of variation CV = 100s/⊽
14. Compare the systematic error (inaccuracy) and
random error (imprecision) with the values
in the performance specifications (p. 49) or
the specifications of your own laboratory. If
the results fall within the specifications, the
pipette is ready for use. Otherwise, check
both systematic and random errors and, when
necessary, proceed to recalibrate (Chapter
9.2).
NOTE: Systematic error (inaccuracy) is the
difference between the dispensed volume and the
selected test volume. Random error (imprecision)
is the scatter of the dispensed volumes around
the mean of the dispensed volume. (ISO 8655-1.)
NOTE: Sartorius specifications are achieved in
strictly controlled conditions (ISO 8655-6). The
user should establish own specifications based on
the field of use and the accuracy requirements
placed on the pipette (ISO8655-1).
NOTE: This method is based on ISO 8655.
Σ