Siemens ADVIA 2120 Manual

ADVIA® 2120/2120i Hematology Systems
Operator’s Guide
067D0157-01 Rev. , 201-0

© 2013–2019 Siemens Healthcare Diagnostics. All rights reserved.
No part of this operator’s guide or the products it describes may be reproduced by
any means or in any form without prior consent in writing from
Siemens Healthcare Diagnostics.
ADVIA, OPTIpoint, and TIMEPAC are trademarks of
Siemens Healthcare Diagnostics.
Acrobat is a trademark of Adobe Systems Incorporated.
Carbon Copy is a trademark of Altiris.
Clorox is a trademark of the Clorox Co.
Exetainer is a trademark of Labco, Ltd.
HEWLETT is a trademark of Hewlett-Packard Company.
HEMOGARD and VACUTAINER are trademarks of Becton-Dickinson
Corporation.
Intel and Pentium are trademarks of Intel Corporation.
Loctite is a trademark of Loctite Corporation.
Lysol is a trademark of National Laboratories.
Microsoft, Windows NT, and Windows are trademarks of Microsoft Corporation.
Monoject is a product of Sherwood Medical.
Monovette is a trademark of Sarstedt Incorporated.
Nokia is a trademark of Nokia Corporation.
Tapval is a trademark of Aquisel S.L.
Teflon is a trademark of E.I. DuPont de Nemours.
Vacuette is a trademark of C.A. Greiner & Söhne.
Venoject is a trademark of Terumo Medical.
Zip is a trademark of Iomega Corporation.
The information in this operator’s guide was correct at the time of printing.
However, Siemens continues to improve products and reserves the right to change
specifications, equipment, and maintenance procedures at any time without notice.
If the system is used in a manner differently than specified by Siemens, the
protection provided by the equipment may be impaired. See warning and hazard
statements.

Contents i
Contents
CONTENTS ...................................................................................................................... I
WELCOME TO THE ADVIA 2120 HEMATOLOGY SYSTEM.......................... 1-1
OVERVIEW .................................................................................................................. 1-2
COMPONENTS .............................................................................................................. 1-3
HOW THE ADVIA 2120 HEMATOLOGY SYSTEM WORKS .......................................... 1-14
HOW THE ADVIA 2120 SOFTWARE WORKS ............................................................. 1-15
RESULTS .................................................................................................................... 1-18
SPECIFICATIONS ........................................................................................................ 1-19
TURNING THE SYSTEM ON / OFF ......................................................................... 2-1
OVERVIEW .................................................................................................................. 2-2
TURNING THE SYSTEM ON............................................................................................ 2-2
TURNING THE SYSTEM OFF .......................................................................................... 2-4
DAILY ROUTINE ........................................................................................................ 3-1
STARTING EACH SHIFT ................................................................................................ 3-2
PROCESSING THE SAMPLES .......................................................................................... 3-5
ENDING EACH SHIFT ................................................................................................. 3-10
MAINTAINING THE ANALYZER ........................................................................... 4-1
SCHEDULE ................................................................................................................... 4-2
SYSTEM WASH ............................................................................................................ 4-3
CLEANING THE CENTERING COLLAR ........................................................................... 4-3
CLEANING THE SHEAR VALVE AND ASPIRATION PATHWAYS IN THE UFC .................. 4-7
CLEANING THE SHEAR VALVE .................................................................................... 4-7
INSPECTING AND CLEANING THE SYRINGE PLUNGERS .............................................. 4-10
REPLACING THE SAMPLER NEEDLES ......................................................................... 4-12
REPLACING THE SHEATH FILTERS ............................................................................. 4-15
REPLACING THE 50 OR 1000-µL SYRINGE PLUNGERS ................................................ 4-16
CLEANING THE AIR-CIRCULATION FILTER ................................................................ 4-19
INSPECT THE PEROX-CAP VENT HOLE FOR BUILDUP ................................................ 4-20
CLEANING THE AUTOSAMPLER ASPIRATE ASSEMBLY ............................................... 4-20
TROUBLESHOOTING THE ANALYZER ............................................................... 5-1
ALIGNMENTS AND ADJUSTMENTS ............................................................................... 5-2
CLEANING PROCEDURES ............................................................................................. 5-5
REPAIR AND REPLACEMENT ...................................................................................... 5-24
TROUBLESHOOTING TIPS ........................................................................................... 5-45
FLAGS ........................................................................................................................... 6-1
MORPHOLOGY FLAGS .................................................................................................. 6-3
SAMPLE/SYSTEM FLAGS ............................................................................................ 6-21
STATUS-LINE MESSAGES ....................................................................................... 7-1
METHODS .................................................................................................................... 8-1
BASOPHIL / LOBULARITY METHOD ............................................................................. 8-3
CSF METHOD ............................................................................................................ 8-16
HEMOGLOBIN METHOD ............................................................................................. 8-21

ii Contents
PEROXIDASE METHOD ............................................................................................... 8-25
RBC / PLATELET METHOD ........................................................................................ 8-37
RETICULOCYTE METHOD .......................................................................................... 8-58
REGULATORY INFORMATION ............................................................................. 9-1
METHODS INTRODUCTION ........................................................................................... 9-4
CLSI DOCUMENT M29-A3 AND SIEMENS METHOD TOPICS CROSS REFERENCE ....... 9-18
CBC METHOD ........................................................................................................... 9-21
CSF METHOD ............................................................................................................ 9-33
WBC DIFF METHOD ................................................................................................ 9-45
RETICULOCYTE METHOD .......................................................................................... 9-54
METHOD DATA SUMMARY ........................................................................................ 9-62
ADVIA AUTOSLIDE® SLIDE MAKER STAINER ............................................... 10-1
OVERVIEW ................................................................................................................ 10-2
AUTOSLIDE SAFETY INFORMATION AND WARNINGS ................................................. 10-5
THEORY OF OPERATION ............................................................................................ 10-8
AUTOSLIDE DAILY ROUTINE ..................................................................................... 10-9
AUTOSLIDE OPERATION .......................................................................................... 10-10
LOADING REAGENTS ............................................................................................... 10-10
LOADING SLIDE RACKS ........................................................................................... 10-11
LOADING SLIDES ..................................................................................................... 10-11
EMPTYING THE STAIN WASTE CONTAINER ............................................................. 10-11
RUNNING THE DAILY STARTUP MANUALLY ............................................................ 10-12
ORDERING A SLIDE MANUALLY .............................................................................. 10-12
STAINING MANUALLY SMEARED SLIDES .................................................................. 10-12
RESETTING THE AUTOSLIDE MODULE ..................................................................... 10-13
STOPPING THE AUTOSLIDE MODULE (FAST STOP) .................................................. 10-13
RUNNING THE DAILY SHUTDOWN MANUALLY ....................................................... 10-13
DISCONNECTING AN AUTOSLIDE 90° CONFIGURATION……………………………10-14
AUTOSLIDE METHODS ............................................................................................. 10-18
PERIODIC MAINTENANCE ........................................................................................ 10-32
ERROR MESSAGES AND SUGGESTED ACTIONS ........................................................ 10-42
AUTOSLIDE SPECIFICATIONS ................................................................................... 10-47
LEGAL INFORMATION ........................................................................................... A-1
LIMITED INSTRUMENT WARRANTY AND SERVICE DELIVERY POLICY ........................ A-2
WARRANTY AND SERVICE EXCLUSIONS ...................................................................... A-4
CONTACT INFORMATION ............................................................................................ A-5
WARNINGS AND SAFETY INFORMATION .........................................................B-1
WARNINGS ..................................................................................................................B-2
SAFETY INFORMATION ................................................................................................B-3
REGULATORY COMPLIANCE ........................................................................................B-3
DOCUMENTATION .......................................................................................................B-4
SYSTEM SYMBOLS .......................................................................................................B-4
INTERPRETATION OF RESULTS .....................................................................................B-9
EXPLANATION OF THE WARNING LABELS ON THE AC POWER BOX ............................ B-10
EXPLANATION OF THE WARNING LABELS ON THE MANUAL CLOSED-TUBE
SAMPLER ................................................................................................................... B-11
PROTECTING YOURSELF FROM LASERS ...................................................................... B-12

Welcome to the ADVIA 2120/2120i Hematology System 1-1
Welcome to the ADVIA®2120/2120i Hematology System
OVERVIEW................................................................................................................................................. 2
COMPONENTS ........................................................................................................................................... 3
AUTOSAMPLER............................................................................................................... 4
MANUAL CLOSED-AND OPEN-TUBE SAMPLERS............................................................. 5
UNIFIED FLUIDS CIRCUIT (UFC).................................................................................... 5
OPTICAL ASSEMBLIES .................................................................................................... 7
SAMPLE,SHEATH,AND DIAPHRAGM PUMPS ................................................................ 10
REAGENTS,SHEATH /RINSE, WASH,AND DEFOAMER......................................... 12
VACUUM AND PRESSURE REGULATOR KNOBS............................................................. 12
TOUCHPAD ................................................................................................................... 12
TOUCHSCREEN ............................................................................................................. 13
MANUAL BARCODE READER ........................................................................................ 13
WASTE REMOVAL SYSTEM ........................................................................................... 14
HOW THE ADVIA 2120/2120I HEMATOLOGY SYSTEM WORKS ................................................ 15
HOW THE ADVIA 2120/2120I SOFTWARE WORKS......................................................................... 16
STATUS LINES............................................................................................................... 16
MENUS ......................................................................................................................... 17
TABS ............................................................................................................................ 18
LEFT-SIDE BUTTONS ..................................................................................................... 18
MAIN DISPLAY AREA .................................................................................................... 18
BOTTOM BUTTONS........................................................................................................ 18
HELP BUTTON............................................................................................................... 18
SHORTCUT KEYS........................................................................................................... 18
WIZARDS...................................................................................................................... 18
PRINTING THE SCREEN.................................................................................................. 19
RESULTS.................................................................................................................................................... 20
SPECIFICATIONS.................................................................................................................................... 21
AUTOSAMPLER............................................................................................................. 21
DATA MANAGEMENT ................................................................................................... 21
PARAMETERS................................................................................................................ 22
PERFORMANCE SPECIFICATIONS................................................................................... 23
PERSONAL COMPUTER SPECIFICATIONS (MINIMUM) .................................................... 23
PHYSICAL SPECIFICATIONS........................................................................................... 24
SAMPLE MODE VOLUMES.............................................................................................. 25
TEST SELECTIVITY /THROUGHPUT............................................................................... 25

1-2 Welcome to the ADVIA 2120/2120i Hematology System
Overview
The ADVIA®2120/2120i Hematology System is a fully automated diagnostic
instrument with a throughput of 120 samples per hour (CBC/diff). The analyzer
uses whole blood samples to provide the following types of results:
•Complete blood counts (CBC)
•CBC plus white cell differential counts (CBC/diff)
•Reticulocyte absolute, percent and indices counts (retic)
•CBC/diff plus retic (CBC/diff/retic)
•CBC/retic

Welcome to the ADVIA 2120/2120i Hematology System 1-3
Components
The ADVIA 2120/2120i Hematology System is made up of two major
components:
•The analyzer contains all the electronics, pneumatics, hydraulics, and
sampler mechanisms, as well as the on-board reagent storage for all reagents,
DEFOAMER, and wash solutions except SHEATH / RINSE. The waste
system is a subcomponent of the analyzer.
1 Autosampler
2 Manual open-tube sampler
3 Manual closed-tube sampler
4 Unifluidics™technology
5 Perox optical assembly
6 RBC optical assembly
7 Hgb colorimeter assembly
8 Sample, sheath, and diaphragm
pumps
9 Reagents, sheath, wash, and rinse
10 Vacuum and pressure regulator
knobs
11 Touchpad
12 Manual barcode reader
13 Waste removal system
•The personal computer includes a monitor, a mouse, a keyboard, and the
interconnect cabling.

1-4 Welcome to the ADVIA 2120/2120i Hematology System
Autosampler
The autosampler automatically transports, mixes, identifies, and aspirates
samples in closed tubes.
The autosampler is a rack-style sampler with a capacity of 150 sample tubes: 15
racks of 10 tubes. The individual racks are barcoded for rack and position
numbers.
Allowable tube sizes
Tube size (mm)
diameter x height Manufacturer
Draw
Vol. (mL)
Minimum Vol.
Required
Dead Vol. (mL)
10 x 50 Becton-Dickinson
VACUTAINER -- 0.6
10 x 64 Venoject -- 0.6
11 x 65 Sarstedt Monovette 2.7 1.0
11 x 74 Tapval 4 1.1
11 x 78 screw cap 4 1.2
11 x 83 Sarstedt Monovette 4 1.0
11 x 91 Sarstedt Monovette 5 1.0
12 x 75.6 Greiner Vacuette 2, 4 1.0
12 x 80 screw cap -- 1.1
12 x 80.7 Exetainer 3 1.0
13 x 100 Becton-Dickinson
HEMOGARD 7 0.8
13 x 100 Greiner Vacuette 6
13 x 74 Monoject 5 0.6
13 x 74 Complexon 5 0.8
13 x 75 Becton-Dickinson
HEMOGARD 2, 3, 5 0.8
13 x 75 Becton-Dickinson
VACUTAINER 7 0.6
13 x 75 Becton-Dickinson
VACUTAINER 5 0.6
13 x 75 Lip-Vac 4
13 x 78 Venoject II 5 1.5
Some of the allowable tube closure types
•Standard VACUTAINER
•HEMOGARD
•Center puncture Safety-Monovette

Welcome to the ADVIA 2120/2120i Hematology System 1-5
•Venoject II
Manual Closed- and Open-tube Samplers
1
2
3
Single samples and STAT samples are aspirated from the manual samplers.
In closed-tube aspiration, the capped vial is inserted upside down into the
centering collar (1). The downward pressure of inserting the tube into the
centering collar will cause the needle to push up, puncture the tube closure, and
aspirate a sample.
In open-tube aspiration, the sample probe (2) is immersed into the specimen.
Pressing the push-to-aspirate plate (3) starts the sampling.
Sample identification and the type of test requested is entered by the manual
barcode reader or from the Manual Sample ID tab before starting sample
aspiration.
Unified Fluids Circuit (UFC)
The UFC assembly uses Unifluidics™
technology.
The UFC block is made up of eight acrylic
plates. Machined within these plates are the
pathways for the fluids and air flow, valves,
and four reaction chambers. The perox
reaction chamber is mounted on the outside
surface of the UFC block.
The reagent pump assembly, mounted to the
bottom of the UFC block, is also acrylic. The
pump has one membrane with seven
individual pump areas that act as diaphragms
that force the reagents into the reaction
chambers.
1
2
3
4
5
6
7
1 Hgb Reaction Chamber
(Not Visible) 4 Retic Reaction Chamber
5 Perox Reaction Chamber

1-6 Welcome to the ADVIA 2120/2120i Hematology System
2 Baso Reaction Chamber
3 RBC Reaction Chamber
6 Shear Valve
7 Reagent Pump Assembly
Shear valve
The shear valve (1) is made up of two ceramic disks.
The rear disk (2) is stationary. The front disk (3) rotates
to “shear” or divide the sample into appropriate aliquots
for analysis.
The front disk also rotates to allow aspiration for direct
cytometry.
(side view of the UFC)
1
3
2
Reaction chambers
The reaction chambers are machined pockets
in the UFC assembly where the sample and
reagents are mixed and the cytochemical
reaction takes place.
There are five reaction chambers.
1 Hgb Reaction Chamber
(Not Visible)
2 Baso Reaction Chamber
3 RBC Reaction Chamber
4 Retic Reaction Chamber
5 Perox Reaction Chamber
1
2
3
4
5
The perox reaction chamber (5) is temperature controlled. The mixture of sample
and three reagents, PEROX 1, PEROX 2, and PEROX 3, is heated to achieve the
desired cytochemical reaction.

Welcome to the ADVIA 2120/2120i Hematology System 1-7
The baso/lobularity reaction chamber (2) is temperature controlled. The mixture
of sample and reagent is heated to achieve the desired cytochemical reaction.
The Hgb reaction chamber (1) serves as an optical cuvette in which the
hemoglobin measurement is read.
The RBC/Plt and retic reaction chambers (3) and (4) serve as containers in which
the reagents and samples are mixed for the desired cytochemical reaction.
Optical Assemblies
12
3
The perox optical assembly (1) directs light from a tungsten-halogen lamp to the
perox flowcell. The use of a sheath stream in the flowcell allows a cell-by-cell
measurement of light scatter and absorption.
The hemoglobin colorimeter assembly (2) takes voltage readings that correspond
to the amount of transmitted light that passes through the reaction chamber. The
system uses the readings to derive the hemoglobin concentration.
The laser optical assembly (3) uses a laser diode light source. This optical
assembly is shared by the RBC/Plt, retic, and baso/lobularity channels. The use
of a sheath stream in the flowcell allows cell-by-cell measurement of low- and
high-angle light scattering and absorption.
Perox Optical Assembly
The perox optical assembly measures scattering and absorption of a tungsten
light beam as it passes through a stream of prepared white blood cells in a
flowcell.
ADE
G
I
BCFH
A Tungsten Lamp D Sample Stream
E Filter
G Absorption PC Board
H Dark Field Stop

1-8 Welcome to the ADVIA 2120/2120i Hematology System
B Slit Aperture
C Circular Aperture
F Beam Splitter I Scatter PC Board
1. The image of a rectangular slit illuminated by the light from the tungsten
lamp is focused into the flowcell and onto the photodiodes on the scatter and
absorption PC boards. The detected signal pulses from cells passing through
the slit image are proportional to the optical power scattered into defined
angles and the optical power absorbed or lost. The resulting scattering and
absorption of the light from each white blood cell is due to the size and
staining characteristics of each cell.
2. The dark field stop intersecting the light beam going to the scatter
photodiode only accepts light scattered at angles between 5°and 10°.
3. The absorption signals are collected over a 0°and 10°angular interval.
4. The photodiode converts the optical scatter and absorption signals from each
white blood cell into two signal currents for each of the two channels (scatter
and absorption). Preamplifiers convert these optical signals into signal
voltages.
Laser Optical Assembly
A
D
A
N
G
E
R
W33
W32
B
C
A Illuminator
Assembly
B Flowcell
Location
C Detection
Assembly
The laser optical assembly consists of the illuminator, flowcell, and detector
assemblies. A laser diode, housed in the illuminator assembly, is used as the light
source.
The image of a slit illuminated by light from the laser diode is focused into the
flowcell. The sample/sheath stream in the flowcell contains iso-volumetrically
sphered red blood cells (RBC).
The RBCs and reticulocytes that pass through the slit image in the flowcell
scatter light at low and high angles; the stained reticulocytes also absorb a
percentage of the light. The scattered light is detected by the two scatter
photodiodes and generates the following signals:
•A high-angle scatter signal corresponding to light scattered at angles between
5°and 15°

Welcome to the ADVIA 2120/2120i Hematology System 1-9
•A low-angle scatter signal corresponding to light scattered at angles between
2°and 3°
A
B
C
DE
G
I
FH
J
A Laser Diode Driver
Board
B Laser Diode
C Sample Stream
D Hi-NA Lens
E Beam Splitter
F Absorption Detector
G Asymetrical Dark Stop
H Low Angle Scatter
Detector
I Beam Divider
J High Angle Scatter
Detector
Hemoglobin Colorimeter Assembly
B
A
C
A Lamp Assembly B Hgb Reaction Chamber C UFC Assembly
The hemoglobin colorimeter assembly straddles the UFC block (C) at the top.
The colorimeter contains a light source (A) set at 3.5 Vdc and a 565 nm or 546 nm
interference filter, according to the HGB method selected.
The hemoglobin reaction chamber (B) is built into the UFC assembly.
Hemoglobin concentration is calculated using baseline and sample readings taken
at specific intervals during the hemoglobin sample analysis period.

1-10 Welcome to the ADVIA 2120/2120i Hematology System
These voltage readings correspond to the amount of transmitted light that passes
through the reaction chamber when it contains sample mixed with reagent or
rinse.
The voltage readings are then converted to a digital form by the Hgb Interface
Board and sent to the analyzer CPU to calculate the optical density and derive the
Hgb concentration.
Sample, Sheath, and Diaphragm Pumps
2
13
4
The perox sample (1) and sheath (2) pumps are located on the left side of the analyzer.
The RBC/baso/retic sample (3) and sheath (4) pumps are located on the right side
of the analyzer.
The diaphragm pumps for sheath, rinse, and wash (five in all) are located above
the reagent containers.

Welcome to the ADVIA 2120/2120i Hematology System 1-11
The sample pumps (1)
dispense an exact amount
of reacted sample (2) from
the appropriate reaction
chamber (3) into the
flowcells (4).
The sheath diaphragm
pumps (5) push the sheath
(6) through the sheath
filters (7) directly into the
flowcells.
The sheath pumps (8) pull
the PEROX SHEATH or
SHEATH / RINSE and the
reacted sample stream (9)
through the flowcells for
analysis.
The analyzed sample and
sheath are sent to waste
(10) and the lines,
flowcells, and reaction
chambers are washed and
rinsed.
V15
V8
V5
V6 V7
V16
V17
V18
DP1 (V26)
V24
V25
3
81
7
4
5
6
10 10
9
22
2
2
2

1-12 Welcome to the ADVIA 2120/2120i Hematology System
Reagents, SHEATH / RINSE, Wash, and DEFOAMER
Reagent is mixed with a sample for cytochemical analysis and measurement.
Sheath is a fluid that envelops the sample stream as it passes through the optics,
ensuring cell-by-cell analysis.
Rinse cleans the hydraulic pathways and reaction chambers after each sample to
prevent carryover and ensure the integrity of the results.
Wash solution removes buildup in the hydraulic pathways. A system wash
should be performed periodically: once a day or after a set number of samples.
DEFOAMER reduces foam buildup in the waste container.
Wash, PEROX SHEATH, DEFOAMER, and all cytochemical reagents are
located on the analyzer and, except for the SHEATH / RINSE and DEFOAMER,
they are monitored on a cycle-by-cycle basis.
The SHEATH / RINSE is in a CUBITAINER®placed on the floor.
Vacuum and Pressure Regulator Knobs
20 In.-Hg
Vacuum Regulator
Knob
40 PSI
Pressure Regulator
Knob
5 PSI
Pressure Regulator
Knob
20 PSI
Pressure Regulator
Knob
Touchpad
Start/Stop
Sampler Eject
Rack Rack
in Sampler
Standby
On
Off
The touchpad, located on the lower right of the analyzer, is the means by which
you operate the analyzer.
On – Provides power to the analyzer assemblies and power supplies. After On is
pressed, samples through the manual open- and closed-tube samplers can be
processed within 2.5 minutes, while samples through the autosampler can begin
processing within four minutes.
Standby – Sets the analyzer to a lower power state. To exit, press Standby.

Welcome to the ADVIA 2120/2120i Hematology System 1-13
Start/Stop Sampler – Starts the autosampler operation from the Ready to Run
mode or stops the autosampler. When the autosampler operation is stopped,
samples underway are allowed to finish.
Eject Rack – Moves all racks within the autosampler into the output queue. The
analysis of the last sample aspirated is completed.
Rack in Sampler – Lights up when a sample rack is in the autosampler,
including the input queue.
Off – Turns off the power to all analyzer assemblies and power supplies, except
the touchpad.
Touchscreen
The touchscreen monitor makes it possible for you to perform your daily tasks
directly at the monitor without using the keyboard.
On the touchscreen monitor, you can do the following:
•Use your finger, with or without gloves, to select all window controls,
including function buttons and operations in the online Help.
•Move a window by holding your finger on the title bar of the window,
drag the window to the new location.
NOTE: The touchscreen may not responds correctly if your finger or gloves are
wet.
You can clean the touchscreen monitor using a lint free paper towel dampened
with 5% bleach solution. Dry the screen and your hands throroughly.
Manual Barcode reader
The manual barcode reader is used to enter information from labels on sample
tubes, reagent containers, controls, and calibrators. As each label is correctly
read, the LED on the wand will blink.

1-14 Welcome to the ADVIA 2120/2120i Hematology System
Waste removal system
All analyzer waste is stored in an 11-liter waste container. This container can be
a stand-alone container, which has to be emptied manually, or part of the
automatic waste removal system. When the fluid level in either of the containers
reaches the maximum-level line (approximately 8 liters), an error message
appears on the Status line on the personal computer monitor. The analyzer will
not aspirate any more samples until the waste container is emptied.

Welcome to the ADVIA 2120/2120i Hematology System 1-15
Stand-alone waste container Automatic Waste Removal
How the ADVIA 2120/2120i Hematology System Works
Blood samples can be aspirated through the:
•Autosampler
•Manual closed-tube sampler
•Manual open-tube sampler
After a sample (4) is aspirated, it is drawn into the shear valve (5). As the shear
valve rotates, it “shears” or divides the sample into aliquots for the different types
of tests.
24
1
2
3
4
5
6
7
8
9
10
11
12
13
14
1516 17 18 19 20 21 2223
BASO
RETIC
RBC
HGB
PEROX
5
4
V74
V2
V72
V73
V47
V1
Rinse Trapped in Lines
Lines that are Dry
Lines with Sample
Line with Vacuum
Conductivity Detector
Rinse Source
Probe
Vacuum Source
The reagents and sample segments are delivered to their respective reaction
chambers for mixing and aspiration. (PEROX 2 and PEROX 3 reagents are
delivered directly to the perox reaction chamber.)

1-16 Welcome to the ADVIA 2120/2120i Hematology System
Once the cytochemical reactions are complete in the reaction chambers, the
sample and reagent mixtures from the perox, RBC, baso, and retic reaction
chambers are sent to the flowcells for analysis.
The Hgb reaction chamber serves as an optical cuvette through which the
hemoglobin measurement is read.
After analysis, the sample and reagent mixture is evacuated into the waste
container and the appropriate pathways and reaction chambers are rinsed.
Test results are sent to the computer to be reviewed and edited.
How the ADVIA 2120/2120i Software Works
The ADVIA 2120/2120i system software is a special-purpose program that runs
on the Windows NT operating system. You can navigate through the software
and operate the system using the mouse.
1 Status lines
2 Menus
3 Tabs
4 Left-side
buttons
5 Main display
area
6 Bottom
buttons
7 Help button
8 Shortcut keys
Status lines
There are two status lines that display messages about the system. Both lines
consist of two parts, each displaying different information.
The system saves all messages in the message log. Click the
message icon to view the last 10 messages.
First Status Line
Left Side: Next sample ID, sample type, and test requested
Right Side: Service, Notepad, Help, Printer, and Autoslide icons