Sutter Instrument Lambda 10-2 Installation manual

One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: .sutter.com
Lambda 10
Lambda 10Lambda 10
Lambda 10-
--
-2
22
2
Optical Filter Changer
Optical Filter Changer Optical Filter Changer
Optical Filter Changer
Control
Control Control
Control System
SystemSystem
System
Operation Manual
Operation ManualOperation Manual
Operation Manual
Rev. 2.04 ( 20120224)

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
ii
Copyright © 2011 Sutter Instrument Company. All Rights Reserved.
LAMB A 10
®
is a registered trademark of Sutter Instrument Company.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
iii
ISCLAIMER
ISCLAIMERISCLAIMER
ISCLAIMER
The LAMB A 10
LAMB A 10 LAMB A 10
LAMB A 10-
--
-2
22
2 Optical Filter Changer system is designed for the specific use of
changing filters into and out of an optical pathway and no other use is recommended.
This instrument is designed for use in a laboratory environment. It is not intended for use,
nor should it be used, in human experimentation or applied to humans in any way. This is
not a medical device.
o not open or attempt to repair the instrument. Extreme heat and high voltages are
present and could cause injury.
o not allow unauthorized and/or untrained operative to use this device.
Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company
assumes no implied or inferred liability for direct or consequential damages from this
instrument if it is operated or used in any way other than for which it is designed.
SAFETY WARNINGS AN
SAFETY WARNINGS AN SAFETY WARNINGS AN
SAFETY WARNINGS AN PRECAUTIONS
PRECAUTIONSPRECAUTIONS
PRECAUTIONS
Electrical
ElectricalElectrical
Electrical
Operate the Lambda 10
Operate the Lambda 10Operate the Lambda 10
Operate the Lambda 10-
--
-2 using 110
2 using 1102 using 110
2 using 110-
--
-120 V AC, 60 Hz, or 220
120 V AC, 60 Hz, or 220120 V AC, 60 Hz, or 220
120 V AC, 60 Hz, or 220-
--
-240 V AC., 50 Hz line
240 V AC., 50 Hz line 240 V AC., 50 Hz line
240 V AC., 50 Hz line
voltage. This instrument is designe
voltage. This instrument is designevoltage. This instrument is designe
voltage. This instrument is designed for connection to a standard laboratory power
d for connection to a standard laboratory power d for connection to a standard laboratory power
d for connection to a standard laboratory power
outlet (Overvoltage Category II), and because it is a microprocessor
outlet (Overvoltage Category II), and because it is a microprocessoroutlet (Overvoltage Category II), and because it is a microprocessor
outlet (Overvoltage Category II), and because it is a microprocessor--
----
--controlled device,
controlled device, controlled device,
controlled device,
it should be accorded the same system wiring precautions as any 'computer type'
it should be accorded the same system wiring precautions as any 'computer type' it should be accorded the same system wiring precautions as any 'computer type'
it should be accorded the same system wiring precautions as any 'computer type'
system. A surge protector and power reg
system. A surge protector and power regsystem. A surge protector and power reg
system. A surge protector and power regulator are recommended.
ulator are recommended.ulator are recommended.
ulator are recommended.
Fuse Replacement: Replace only with the same type and rating:
Fuse Replacement: Replace only with the same type and rating: Fuse Replacement: Replace only with the same type and rating:
Fuse Replacement: Replace only with the same type and rating:
Fuse
FuseFuse
Fuse:
: :
: (Time elay, 5mm x 20mm, glass tube)
(Time elay, 5mm x 20mm, glass tube)(Time elay, 5mm x 20mm, glass tube)
(Time elay, 5mm x 20mm, glass tube)
Voltage
Voltage Voltage
Voltage
Setting
SettingSetting
Setting
Type and Rating
Type and RatingType and Rating
Type and Rating
Manufacturer Examples
Manufacturer ExamplesManufacturer Examples
Manufacturer Examples
110V
110V110V
110V
1.5A, 250V
1.5A, 250V1.5A, 250V
1.5A, 250V
Bussmann GMC
Bussmann GMCBussmann GMC
Bussmann GMC-
--
-1.5A or GMC
1.5A or GMC1.5A or GMC
1.5A or GMC-
--
-1.5
1.51.5
1.5-
--
-R (RoHS)
R (RoHS)R (RoHS)
R (RoHS)
220V
220V220V
220V
T0.63A, 250V
T0.63A, 250VT0.63A, 250V
T0.63A, 250V
Bussmann G C
Bussmann G CBussmann G C
Bussmann G C-
--
-630mA or S506
630mA or S506630mA or S506
630mA or S506-
--
-630
630630
630-
--
-R (RoHS); or
R (RoHS); orR (RoHS); or
R (RoHS); or
Littelfuse: 218.630 or 218.063P (RoHS)
Littelfuse: 218.630 or 218.063P (RoHS)Littelfuse: 218.630 or 218.063P (RoHS)
Littelfuse: 218.630 or 218.063P (RoHS)
A spare fuse is located in the power input module. Please refer to the fuse
A spare fuse is located in the power input module. Please refer to the fuseA spare fuse is located in the power input module. Please refer to the fuse
A spare fuse is located in the power input module. Please refer to the fuse-
--
-replacement
replacement replacement
replacement
appendix for more details on fuse ratings and for i
appendix for more details on fuse ratings and for iappendix for more details on fuse ratings and for i
appendix for more details on fuse ratings and for instructions on how to change the
nstructions on how to change the nstructions on how to change the
nstructions on how to change the
fuse.
fuse. fuse.
fuse.
Avoiding Electrical Shock and Fire
Avoiding Electrical Shock and FireAvoiding Electrical Shock and Fire
Avoiding Electrical Shock and Fire-
--
-related Injury
related Injuryrelated Injury
related Injury
Always use the grounded power supply cord set provided to connect the system to
Always use the grounded power supply cord set provided to connect the system to Always use the grounded power supply cord set provided to connect the system to
Always use the grounded power supply cord set provided to connect the system to
a grounded outlet (3
a grounded outlet (3a grounded outlet (3
a grounded outlet (3-
--
-prong).
prong). prong).
prong). This is required to protect you from injury in the event
This is required to protect you from injury in the event This is required to protect you from injury in the event
This is required to protect you from injury in the event
that an electrical hazard occurs.
that an electrical hazard occurs.that an electrical hazard occurs.
that an electrical hazard occurs.
o not disassemble the system. Refer servicing to qualified personnel.
o not disassemble the system. Refer servicing to qualified personnel.o not disassemble the system. Refer servicing to qualified personnel.
o not disassemble the system. Refer servicing to qualified personnel.
To prevent fire or shock hazard do not expose the unit to rain or moisture.
To prevent fire or shock hazard do not expose the unit to rain or moisture.To prevent fire or shock hazard do not expose the unit to rain or moisture.
To prevent fire or shock hazard do not expose the unit to rain or moisture.
Optical Radiatio
Optical RadiatioOptical Radiatio
Optical Radiation
nn
n
This system is designed for use in conjunction with high
This system is designed for use in conjunction with highThis system is designed for use in conjunction with high
This system is designed for use in conjunction with high-
--
-intensity light sources. Failure to
intensity light sources. Failure to intensity light sources. Failure to
intensity light sources. Failure to
comply with any of the following precautions may result in injury to the users o
comply with any of the following precautions may result in injury to the users ocomply with any of the following precautions may result in injury to the users o
comply with any of the following precautions may result in injury to the users of this
f this f this
f this
device as well as those working in the general area near the device.
device as well as those working in the general area near the device.device as well as those working in the general area near the device.
device as well as those working in the general area near the device.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
iv
Never look into the optical pathway of the high intensity light sources typically
Never look into the optical pathway of the high intensity light sources typically Never look into the optical pathway of the high intensity light sources typically
Never look into the optical pathway of the high intensity light sources typically
used with this instrument. oing so can cause permanent eye damage.
used with this instrument. oing so can cause permanent eye damage.used with this instrument. oing so can cause permanent eye damage.
used with this instrument. oing so can cause permanent eye damage.
The high
The high The high
The high-
--
-intensity light s
intensity light sintensity light s
intensity light sources typically used with this instrument also produce a
ources typically used with this instrument also produce a ources typically used with this instrument also produce a
ources typically used with this instrument also produce a
significant amount of heat. irect contact with the housing of those instruments can
significant amount of heat. irect contact with the housing of those instruments can significant amount of heat. irect contact with the housing of those instruments can
significant amount of heat. irect contact with the housing of those instruments can
cause serious burns.
cause serious burns.cause serious burns.
cause serious burns.
Electromagnetic Interference
Electromagnetic InterferenceElectromagnetic Interference
Electromagnetic Interference
To comply with F A and CE electromagnetic immunity and interfer
To comply with F A and CE electromagnetic immunity and interferTo comply with F A and CE electromagnetic immunity and interfer
To comply with F A and CE electromagnetic immunity and interference standards; and to
ence standards; and to ence standards; and to
ence standards; and to
reduce the electromagnetic coupling between this and other equipment in your lab always
reduce the electromagnetic coupling between this and other equipment in your lab always reduce the electromagnetic coupling between this and other equipment in your lab always
reduce the electromagnetic coupling between this and other equipment in your lab always
use the type and length of interconnect cables provided with the unit for the
use the type and length of interconnect cables provided with the unit for the use the type and length of interconnect cables provided with the unit for the
use the type and length of interconnect cables provided with the unit for the
interconnection of
interconnection ofinterconnection of
interconnection of one or more f
one or more f one or more f
one or more filter wheels and/or shutters
ilter wheels and/or shuttersilter wheels and/or shutters
ilter wheels and/or shutters, h
, h, h
, host
ost ost
ost computer via serial RS
computer via serial RScomputer via serial RS
computer via serial RS-
--
-
232 or parallel interface
232 or parallel interface232 or parallel interface
232 or parallel interface, or o
, or o, or o
, or other equipment via TTL connections
ther equipment via TTL connectionsther equipment via TTL connections
ther equipment via TTL connections
(
((
(s
ss
see the TECHNICAL
ee the TECHNICAL ee the TECHNICAL
ee the TECHNICAL
SPECIFICATIONS appendix for more details)
SPECIFICATIONS appendix for more details)SPECIFICATIONS appendix for more details)
SPECIFICATIONS appendix for more details).
..
.
Operational
OperationalOperational
Operational
Fai
FaiFai
Failure to comply with any of the following precautions may damage this device.
lure to comply with any of the following precautions may damage this device. lure to comply with any of the following precautions may damage this device.
lure to comply with any of the following precautions may damage this device.
This instrument is designed for operation in a laboratory environment (Pollution
This instrument is designed for operation in a laboratory environment (Pollution This instrument is designed for operation in a laboratory environment (Pollution
This instrument is designed for operation in a laboratory environment (Pollution
egree I).
egree I).egree I).
egree I).
This unit is not designed for operation at altitudes above 2000 meters nor was it test
This unit is not designed for operation at altitudes above 2000 meters nor was it testThis unit is not designed for operation at altitudes above 2000 meters nor was it test
This unit is not designed for operation at altitudes above 2000 meters nor was it tested
ed ed
ed
for safety above 2000 meters.
for safety above 2000 meters.for safety above 2000 meters.
for safety above 2000 meters.
O NOT CONNECT OR ISCONNECT THE CABLES BETWEEN THE
O NOT CONNECT OR ISCONNECT THE CABLES BETWEEN THE O NOT CONNECT OR ISCONNECT THE CABLES BETWEEN THE
O NOT CONNECT OR ISCONNECT THE CABLES BETWEEN THE
CONTROLLER AN THE MECHANICAL UNITS
CONTROLLER AN THE MECHANICAL UNITS CONTROLLER AN THE MECHANICAL UNITS
CONTROLLER AN THE MECHANICAL UNITS (FILTER WHEELS AN /OR
(FILTER WHEELS AN /OR (FILTER WHEELS AN /OR
(FILTER WHEELS AN /OR
SHUTTERS)
SHUTTERS) SHUTTERS)
SHUTTERS) WHILE POWER IS ON.
WHILE POWER IS ON. WHILE POWER IS ON.
WHILE POWER IS ON.
Please allow at least 20 seconds after turning the unit off before disconnecting
Please allow at least 20 seconds after turning the unit off before disconnectingPlease allow at least 20 seconds after turning the unit off before disconnecting
Please allow at least 20 seconds after turning the unit off before disconnecting the
the the
the
mechanical units. Failure to do this may result in damage to the electronics.
mechanical units. Failure to do this may result in damage to the electronics.mechanical units. Failure to do this may result in damage to the electronics.
mechanical units. Failure to do this may result in damage to the electronics.
Operate only in a location where there is a free flow of fresh air on all sides.
Operate only in a location where there is a free flow of fresh air on all sides. Operate only in a location where there is a free flow of fresh air on all sides.
Operate only in a location where there is a free flow of fresh air on all sides.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTE .
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTE .NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTE .
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTE .

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
v
TABLE OF CONTENTS
ISCLAIMER
ISCLAIMERISCLAIMER
ISCLAIMER................................
................................................................
................................................................
................................................................
................................................................
................................................................
................................................................
................................................................
.....................................
..........
..... iii
iiiiii
iii
SAFETY WARNINGS AN PRECAUTIONS
SAFETY WARNINGS AN PRECAUTIONSSAFETY WARNINGS AN PRECAUTIONS
SAFETY WARNINGS AN PRECAUTIONS ................................
................................................................
................................................................
................................................................
................................................
................................
................ iii
iiiiii
iii
Electrical .................................................................................................................................................iii
Avoiding Electrical Shock and Fire-related Injury ............................................................................iii
Optical Radiation ...................................................................................................................................iv
Electromagnetic Interference...............................................................................................................iv
Operational.............................................................................................................................................iv
1. GENERAL
1. GENERAL1. GENERAL
1. GENERAL ................................
................................................................
................................................................
................................................................
................................................................
................................................................
................................................................
................................................................
........................................
................
........1
11
1
1.1 Introduction.......................................................................................................................................1
1.1.1 How to Use this Manual............................................................................................................1
1.1.2 Technical Support......................................................................................................................1
1.2 General escription..........................................................................................................................1
1.2.1 Systems........................................................................................................................................2
1.2.2 Filter Wheels and Shutters.......................................................................................................2
1.2.3 Filters ..........................................................................................................................................4
1.2.4 Slide-In and rop-In Filter Holders ........................................................................................4
1.2.5 Shutters.......................................................................................................................................5
1.2.6 Adapters ......................................................................................................................................5
1.2.7 Other and Optional Accessories ...............................................................................................5
1.2.7.1 Liquid Light Guide (LLG)..................................................................................................5
1.3 Functional escription.....................................................................................................................5
1.3.1 Stepping Motor Operation........................................................................................................5
2. INSTALLATION
2. INSTALLATION2. INSTALLATION
2. INSTALLATION ................................
................................................................
................................................................
................................................................
................................................................
................................................................
.............................................................
..........................................................
.............................7
77
7
2.1 Unpacking..........................................................................................................................................7
2.2 Pre-installation Considerations ......................................................................................................7
2.2.1 Vibrations....................................................................................................................................7
2.3 Installing the Controller ..................................................................................................................8
2.3.1 Line Power (Mains) ...................................................................................................................8
2.4 Installing the Filter Wheel ............................................................................................................10
2.4.1 Filter Wheel Assembly Set Up................................................................................................11
2.5 First Time Use ................................................................................................................................12
2.5.1 Make It Go ................................................................................................................................12
2.6 Loading Filters................................................................................................................................13
2.6.1 Filter Orientation.....................................................................................................................13
2.6.2 Filter Cups and Filter Holders ...............................................................................................13
2.6.3 Installation of Filters into Filter Holders .............................................................................15
2.6.4 Installation of Filters into Filter Cups ..................................................................................17
2.7 Filter Loading Tips.........................................................................................................................18
2.7.1 Selection of a Filter Position...................................................................................................18
2.7.2 Use of Blanking iscs..............................................................................................................18
2.7.3 Neutral ensity Filters ...........................................................................................................19
3.
3.3.
3. OPERATIONS
OPERATIONS OPERATIONS
OPERATIONS ................................
................................................................
................................................................
................................................................
................................................................
................................................................
...............................................................
..............................................................
...............................21
2121
21
3.1 First Time Use ................................................................................................................................21

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
vi
3.2 Principles of Operation ..................................................................................................................21
3.3 Rear Panel Connectors...................................................................................................................21
3.4 Front Panel Controls......................................................................................................................23
3.4.1 Keypad.......................................................................................................................................23
3.4.2 RESET Button .........................................................................................................................23
3.4.3 isplay.......................................................................................................................................23
3.5 Power-Up Sequence........................................................................................................................24
3.5.1 Selecting the Wheel .................................................................................................................25
3.5.2 Selecting the Filter Position ...................................................................................................25
3.5.3 Selecting the Filter Switching Speed.....................................................................................25
3.5.4 Selecting the Shutter Position (Open/Close)........................................................................26
3.5.5 Error etection and Recovery................................................................................................27
3.5.6 Movement Errors: Causes and Solutions.............................................................................27
3.5.6.1 Filter Weight vs. Speed.....................................................................................................27
3.5.6.2 Oscillation when Stopping ...............................................................................................28
3.5.6.3 Oscillations when Moving ................................................................................................28
3.5.6.4 Movement Errors after Successive Moves......................................................................28
3.5.7 Helpful Tips about Movement Errors ...................................................................................28
4.
4.4.
4. EXTERNAL CONTROL OPERATIONS
EXTERNAL CONTROL OPERATIONS EXTERNAL CONTROL OPERATIONS
EXTERNAL CONTROL OPERATIONS ................................
................................................................
................................................................
................................................................
....................................................
........................................
....................31
3131
31
4.1 General.............................................................................................................................................31
4.2 Parallel Interface ............................................................................................................................31
4.2.1 Connecting to the Parallel Port Interface.............................................................................31
4.2.2 Input Command Structure .....................................................................................................32
4.2.2.1 Filter Commands...............................................................................................................33
4.2.2.2 Special Commands: ON LINE, BATCH, and SHUTTER Commands......................34
4.2.2.3 Output Command Structure: BUSY and ERROR lines..............................................36
4.2.3 Strategies for Controlling the Lambda 10-2 via the Parallel Port.....................................37
4.2.3.1 Using Input Lines Only....................................................................................................37
4.2.3.2 Using Fewer than Eight Input Lines..............................................................................37
4.2.3.3 Using the BUSY Line........................................................................................................37
4.2.3.4 Using the ERROR Line ....................................................................................................38
4.2.4 Using a Computer’s Parallel (Printer) Port .........................................................................38
4.2.4.1 Connecting to the PC Parallel (Printer) Port................................................................39
4.2.4.2 Input Command Structure...............................................................................................40
4.2.4.3 Output Command Structure: BUSY and ERROR Lines.............................................41
4.3 edicated TTL Line .......................................................................................................................42
4.4 Serial RS-232 Interface ..................................................................................................................43
4.4.1 Connecting to the Serial Port.................................................................................................43
4.4.2 Input Command Structure .....................................................................................................44
4.4.2.1 Filter Commands...............................................................................................................44
4.4.3 Special Commands: ON LINE, SHUTTER and BATCH commands...............................45
4.4.4 Output Command Structure: Command Echo and <CR>...............................................46
5.
5.5.
5. MAINTENANCE
MAINTENANCE MAINTENANCE
MAINTENANCE ................................
................................................................
................................................................
................................................................
................................................................
................................................................
...........................................................
......................................................
...........................47
4747
47
APPEN IX A.
APPEN IX A.APPEN IX A.
APPEN IX A. LIMITE WARRANTY
LIMITE WARRANTY LIMITE WARRANTY
LIMITE WARRANTY ................................
................................................................
................................................................
................................................................
..........................................................
....................................................
..........................49
4949
49
APPEN IX B.
APPEN IX B.APPEN IX B.
APPEN IX B. ACCESSORIES
ACCESSORIES ACCESSORIES
ACCESSORIES................................
................................................................
................................................................
................................................................
................................................................
................................................................
........................................
................
........51
5151
51
B.1. Filter Wheels..................................................................................................................................51

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
vii
B.2. Shutter............................................................................................................................................51
B.3. Filter Holders and Liquid Light Guides.....................................................................................51
B.4. Cables .............................................................................................................................................52
APPEN IX C.
APPEN IX C.APPEN IX C.
APPEN IX C. FUSE REPLACEMENT
FUSE REPLACEMENT FUSE REPLACEMENT
FUSE REPLACEMENT ................................
................................................................
................................................................
................................................................
.........................................................
..................................................
.........................53
5353
53
APPEN IX .
APPEN IX .APPEN IX .
APPEN IX . TECHNICAL SPECIFICATIONS
TECHNICAL SPECIFICATIONS TECHNICAL SPECIFICATIONS
TECHNICAL SPECIFICATIONS ................................
................................................................
................................................................
................................................................
.........................................
..................
.........55
5555
55
.1. Controller (rack mount)...............................................................................................................55
.2. Filter Wheel, 25 mm (1”) (with Shutter, without Stand)........................................................56
APPEN IX E.
APPEN IX E.APPEN IX E.
APPEN IX E. EXTERNAL CONTROL COMMAN REFERENCE
EXTERNAL CONTROL COMMAN REFERENCE EXTERNAL CONTROL COMMAN REFERENCE
EXTERNAL CONTROL COMMAN REFERENCE ................................
................................................................
.........................................
..................
.........57
5757
57
TABLE OF FIGURES
Figure 1-1. Lambda 10-2 Filter Wheel (without shutter)......................................................................3
Figure 1-2. Lambda 10-2 filter wheel housing (with shutter)...............................................................3
Figure 2-1. Lambda 10-2 Cabinet (rear view). ........................................................................................ 9
Figure 2-2. Lambda 10-2 Cabinet (front view)........................................................................................9
Figure 2-3. Power connection..................................................................................................................10
Figure 2-4. Schematic diagram of two possible experimental configurations...................................10
Figure 2-5. Filter wheel mounting stand...............................................................................................11
Figure 2-6. Filter wheel connection........................................................................................................12
Figure 2-7. Initialization screen..............................................................................................................12
Figure 2-8. Initialization screen in local mode......................................................................................13
Figure 2-9. Filter holder components.....................................................................................................14
Figure 2-10. Removing the retaining ring.............................................................................................15
Figure 2-11. Installing a filter into a Slide-In Filter Holder................................................................16
Figure 2-12. Assembly order of filter holder parts ...............................................................................16
Figure 2-13. Filter ports (plug is removed from the loading port) .....................................................17
Figure 3-1. Electrical connections ..........................................................................................................22
Figure 3-2. Lambda 10-2 Controller Keypad.........................................................................................23
Figure 3-3. Status display screen............................................................................................................24
Figure 3-4. Status display defaults .........................................................................................................24
Figure 3-5. isplay showing selected filter wheel.................................................................................25
Figure 3-6. Screen showing prompt for speed selection.......................................................................26
Figure 3-7. Entering the speed ...............................................................................................................26
Figure 3-8. Status display screen after pressing LOCAL. ...................................................................27
Figure 4-1. Parallel interface B-25 connector on the Lambda 10-2.................................................31

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
viii
Figure 4-2. PC parallel (printer) port.....................................................................................................39
Figure 4-3. Serial port pin assignments.................................................................................................44
Figure 5-1. Power entry module .............................................................................................................53
Figure 5-2. Fuse holder............................................................................................................................54
TABLE OF TABLES
Table 2-1. Maximum allowable filter thickness....................................................................................15
Table 3-1. Filter switching times (in milliseconds)...............................................................................26
Table 4-1. Lambda 10-2 Parallel Interface Pin Assignments..............................................................32
Table 4-2. Filter command structure .....................................................................................................33
Table 4-3. Special command codes..........................................................................................................34
Table 4-4. PC and Lambda 10-2 parallel port pin assignments..........................................................40
Table 4-5. PC printer adapter addresses ...............................................................................................41
Table 4-6. Commands for the dedicated TTL line................................................................................43
Table 4-7. Creating a serial port filter command..................................................................................45
Table C-1. Fuse type and rating. ............................................................................................................54
Table -1. Mains fuse type and rating...................................................................................................55
Table -2. Cable specifications. ..............................................................................................................56
Table E-1. Complete Remote-control command reference..................................................................57
Table E-2. Filter command structure. ...................................................................................................62
TABLE OF LISTINGS
Listing 4-1. Batch transfer via PC parallel port program....................................................................36
Listing 4-2. Program to obtain the Lambda 10-2 status on the PC parallel port.............................41

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
1
1.
1.1.
1. GENERAL
GENERALGENERAL
GENERAL
1.1
1.11.1
1.1 Introduction
IntroductionIntroduction
Introduction
The Lambda 10-2 is an optical filter wheel system designed for rapid change of wavelength
and comprises two subsystems: A controller and one or two filter wheels, each with or
without a shutter, that are to be placed in the optical pathway of your existing experimental
setup. Next is a description on how this manual is organized and the manner in which its use
is intended, followed by a general and functional description of the system.
1.1.1
1.1.11.1.1
1.1.1 How to Use this Manual
How to Use this ManualHow to Use this Manual
How to Use this Manual
This manual is organized in a manner that is best suited for the typical manner in which the
reader would learn about the system, and then install, operate, and maintain it. Prior to the
table of contents of this manual is a disclaimer and a series of cautionary and warning notes
that should be read first. This first chapter provides an overall description of the system,
leaving more in-depth technical information towards the end of the chapter. The next
chapter describes how to install the system, followed by one or more chapters that provide
operation instructions, and ending with a chapter on maintenance. Following the final
chapter are several appendices that provide supplemental information. The installation
chapter covers everything related to installation, from unpacking the equipment as received,
through to ensuring that everything is correctly installed and is operating correctly. The
operation chapters cover the two basic types of user interaction with the system: Manual
(local) control and external computer control. Please take the time to read these instructions
to assure the safe and proper use of this instrument.
1.1.2
1.1.21.1.2
1.1.2 Technical Support
Technical SupportTechnical Support
Technical Support
Unlimited technical support is provided by Sutter Instrument Company at no charge to our
customers. Our technical support staff is available between the hours of 8:00 AM and 5:00
PM (Pacific Time) at (415) 883
(415) 883(415) 883
(415) 883-
--
-0128
01280128
0128. You may also E-mail your queries to [email protected].
1.2
1.21.2
1.2 General escription
General escriptionGeneral escription
General escription
The LAMB A
LAMB ALAMB A
LAMB A 10
1010
10-
--
-2
22
2 is a microprocessor controlled, high-speed optical filter wheel designed
for microscopy and other applications, which require rapid and accurate wavelength
switching. The LAMB A
LAMB ALAMB A
LAMB A 10
1010
10-
--
-2
22
2 supersedes the previous Lambda 10 filter wheel controller
providing enhanced capability while retaining the same outstanding mechanical
performance. The Lambda 10-2 controller can control up to two filter wheels and two high-
speed shutters, and provides for both manual (local) control as well as external computer
control (via either of the provided serial (RS-232) or parallel interfaces).
The controller system is designed around three embedded microcontrollers: one slave for
each wheel and a master for control and communication. The electronics for two wheels and
two shutters are already installed, even if you only ordered a single wheel system. If you add
a second wheel or shutter later, there will be no additional electronics to buy. The embedded
microcontrollers automatically determine the number of filter wheels that are installed, so
there are no jumpers or switches to set.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
2
The controller can run a single wheel and shutter under remote control through a parallel
input using the same simple protocol as the original Lambda 10 controller. New commands
have been added so that the 8-bit parallel input can efficiently control two wheels and two
shutters without using any additional lines. The serial input uses the same command set as
the parallel port, and provides full control of two wheels and two shutters. Either use the
B-25 cable provided to connect to the parallel port or the B-9 cable provided to connect to
the serial port.
The following instructions are meant to help you set up the Lambda 10-2 and become
familiar with the manual mode of filter selection. Further details on controller functionality,
filter installation, and remote communications can be found in other sections of this manual.
1.2.1
1.2.11.2.1
1.2.1 Systems
SystemsSystems
Systems
LB10
LB10LB10
LB10-
--
-2
22
2: Includes one 25mm filter wheel without shutter, support base with mounting rods,
rack mount
1
controller unit with wheel drive cable, parallel and serial interface cables for
connection to PC, two drop-in filter holders, spanner wrench, 10 blanking discs, power cord,
and manual.
LB10
LB10LB10
LB10-
--
-2S
2S2S
2S: Same as above—with a Uniblitz
®2
shutter assembly and two slide-in filter holders.
LB10
LB10LB10
LB10-
--
-232
232232
232: Same as LB10-2 except with 32mm, T-mount threaded filter wheel (no drop-in
filters) and modified 10-2 controller.
LB10
LB10LB10
LB10-
--
-232S
232S232S
232S: Same as LB10-232 except with a Uniblitz
®
shutter (no drop-in or slide-in filters)
and modified 10-2 controller.
LB10
LB10LB10
LB10-
--
-250
250250
250: Same as LB10-2 except with 50mm, 5 position wheel.
1.2.2
1.2.21.2.2
1.2.2 Filter Wheels
Filter WheelsFilter Wheels
Filter Wheels and Shutters
and Shutters and Shutters
and Shutters
When a Lambda 10-2 Filter Wheel system is purchased, its configuration may include one to
two filter wheels, each one with or without a shutter. If the Lambda 10-2 system is
purchased with only one 10-position 25mm (1-inch) filter wheel, you may wish to add a
second filter wheel of the same type later, in which case the second filter wheel can be added
without modification. A 10-position 32mm and a 5-position 50mm filter wheel are also
available for use with the Lambda 10-2 controller. Each port of the controller to which a
32mm or 50mm filter wheel is to be connected, however, must be modified by Sutter
Instrument Co. before said filter wheel can be connected. Note that
Note that Note that
Note that you must
you mustyou must
you must only use filter
only use filter only use filter
only use filter
wheels manufactured by Sutter Instrument.
wheels manufactured by Sutter Instrument. wheels manufactured by Sutter Instrument.
wheels manufactured by Sutter Instrument.
The basic components of the filter wheel mechanical assembly are shown below in Figure 1-1
and Figure 1-2. The only components that are to be removed by the user are the drop-in or
slide in filter holders and the plug in the loading port. At no time should the user remove the
cover plate from the wheel housing.
1
Tabletop controller box is available upon request.
2
Uniblitz
®
is a registered trademark of Vincent Associates.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
3
Figure 1-1. Lambda 10-2 Filter Wheel (without shutter)
Figure 1-2. Lambda 10-2 filter wheel housing (with shutter)
1. 10-position wheel (removed from motor hub
2. Installed filter cup
3. Wheel housing
4. Cover plate (removed from wheel housing
5. Stepper motor
6. rop-in filter holder (Figure 1-1) or slide-in filter holder (Figure 1-2)
7. Mounting ears
8. Optical port
9. Loading port (plug installed)
10. Shutter housing
All filter wheel options are listed in the Sutter Instrument catalogue and in the following
list.
10
1010
10-
--
-position, 25mm filter wheels:
position, 25mm filter wheels:position, 25mm filter wheels:
position, 25mm filter wheels:
LB10
LB10LB10
LB10-
--
-NW
NWNW
NW (without shutter)
LB10
LB10LB10
LB10-
--
-NWS
NWSNWS
NWS (with shutter)

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
4
LB10
LB10LB10
LB10-
--
-NWE
NWENWE
NWE (without shutter and set up for emission)
LB10
LB10LB10
LB10-
--
-W
WW
W (two wheels mounted back to back, without shutter)
LB10
LB10LB10
LB10-
--
-W S
W SW S
W S (two wheels mounted back to back, with one shutter and one slide-in filter
holder)
10
1010
10-
--
-position 32mm filter wheels:
position 32mm filter wheels:position 32mm filter wheels:
position 32mm filter wheels:
LB10
LB10LB10
LB10-
--
-W32
W32W32
W32 (without shutter)
LB10
LB10LB10
LB10-
--
-W32S
W32SW32S
W32S (with shutter)
5
55
5-
--
-position 50mm filter wheel:
position 50mm filter wheel:position 50mm filter wheel:
position 50mm filter wheel:
LB10
LB10LB10
LB10-
--
-W50
W50W50
W50 (without shutter).
NOTE: All shutters mentioned in this manual for use with the Lambda 10
NOTE: All shutters mentioned in this manual for use with the Lambda 10NOTE: All shutters mentioned in this manual for use with the Lambda 10
NOTE: All shutters mentioned in this manual for use with the Lambda 10-
--
-2 are
2 are 2 are
2 are Uniblitz
®
®®
®3
33
3
shutters.
shutters.shutters.
shutters.
1.2.3
1.2.31.2.3
1.2.3 Filters
FiltersFilters
Filters
The Lambda 10-2 filter changer is designed for use with any 1-inch or 25-mm filter with a
thickness that does not exceed the following dimensions:
4.5 mm (0.18 in.) for Slide
4.5 mm (0.18 in.) for Slide4.5 mm (0.18 in.) for Slide
4.5 mm (0.18 in.) for Slide-
--
-in filter holders
in filter holdersin filter holders
in filter holders
5.38 mm (0.21 in.) for rop
5.38 mm (0.21 in.) for rop5.38 mm (0.21 in.) for rop
5.38 mm (0.21 in.) for rop-
--
-in filter holders
in filter holdersin filter holders
in filter holders
9 mm (0.35 in.) for filter cups
9 mm (0.35 in.) for filter cups9 mm (0.35 in.) for filter cups
9 mm (0.35 in.) for filter cups
Filter wheels designed for use with 32 mm filters will accept filters with a thickness that
does not exceed the following dimensions:
9 mm (0.35 in.) for filter cups
9 mm (0.35 in.) for filter cups9 mm (0.35 in.) for filter cups
9 mm (0.35 in.) for filter cups
Instructions for installing filters into the filter wheel can be found in the FILTER WHEEL
chapter of this manual. Filters are not supplied by Sutter Instru
Filters are not supplied by Sutter InstruFilters are not supplied by Sutter Instru
Filters are not supplied by Sutter Instrument. However, filters
ment. However, filters ment. However, filters
ment. However, filters
conforming to the above specifications can be purchased from any filter manufacturer.
conforming to the above specifications can be purchased from any filter manufacturer.conforming to the above specifications can be purchased from any filter manufacturer.
conforming to the above specifications can be purchased from any filter manufacturer.
1.2.4
1.2.41.2.4
1.2.4 Slide
SlideSlide
Slide-
--
-In and rop
In and ropIn and rop
In and rop-
--
-In Filter Holders
In Filter HoldersIn Filter Holders
In Filter Holders
Many users like to have extra Slide-in or rop-in filter holders for more convenient
replacement of these “fixed” filters. Only use filter holders made by Sutter Instrument
Only use filter holders made by Sutter Instrument Only use filter holders made by Sutter Instrument
Only use filter holders made by Sutter Instrument
Company in the Lambda 10
Company in the Lambda 10Company in the Lambda 10
Company in the Lambda 10-
--
-2 Filter Wheel.
2 Filter Wheel. 2 Filter Wheel.
2 Filter Wheel. Extra filter holders are readily available from
Sutter Instrument Company or their distributors and are listed as “SLI E-IN” and “ ROP-
IN” in the Sutter catalogue. At this point, there are no slide-in filter holders for 32 mm
systems, although a 32-mm rop-in filter holder is available. In addition, there is a fixed
filter position for a heat-blocking filter in most 32-mm microscope adapters offered by Sutter
Instrument Company. Slide-in and drop-in filter holders are not available for the 50 mm
filter wheels.
1.2.5
1.2.51.2.5
1.2.5 Shutters
ShuttersShutters
Shutters
If you purchase a Lambda 10-2 Filter Wheel system without a shutter and later decide to add
one to the system, the Filter Wheel must be returned to the factory for the upgrade. The
Lambda 10-2 Filter Wheel controller can support a retrofit shutter without modification.
3
Uniblitz® is a registered trademark of Vincent Associates.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
5
However, you must
you mustyou must
you must only use shutters installed by Sutter Instrume
only use shutters installed by Sutter Instrumeonly use shutters installed by Sutter Instrume
only use shutters installed by Sutter Instrument.
nt. nt.
nt. This upgrade is
available only at Sutter Instrument’s factory (or through their distributors) and is listed as
“SHUTTER” in the Sutter catalogue (or “WHEEL” in older catalogues).
1.2.6
1.2.61.2.6
1.2.6 Adapters
AdaptersAdapters
Adapters
Most microscopes, cameras, light sources and other optical instruments to which the Lambda
10-2 may be interfaced will require some sort of adapter to link the devices. Adapters for
interfacing the filter wheel to most instruments are available from Sutter Instrument or can
be custom built by Sutter Instrument. Suitable adapters are available from other sources,
Suitable adapters are available from other sources, Suitable adapters are available from other sources,
Suitable adapters are available from other sources,
as well, but Sutter Instrument assumes no responsibility for the performance, suitability
as well, but Sutter Instrument assumes no responsibility for the performance, suitability as well, but Sutter Instrument assumes no responsibility for the performance, suitability
as well, but Sutter Instrument assumes no responsibility for the performance, suitability
and safety of adapters built by the user or other manufacturers.
and safety of adapters built by the user or other manufacturers.and safety of adapters built by the user or other manufacturers.
and safety of adapters built by the user or other manufacturers.
1.2.7
1.2.71.2.7
1.2.7 Other and Optional Acce
Other and Optional AcceOther and Optional Acce
Other and Optional Accessories
ssoriesssories
ssories
1.2.7.1 Liquid Light Guide (LLG)
A 2-meter, 3mm diameter liquid light guide is optionally available from Sutter Instrument
(P/N: LLG). The LLG includes a C-mount, lens, and lens tube.
1.3
1.31.3
1.3 Functional escription
Functional escriptionFunctional escription
Functional escription
1.3.1
1.3.11.3.1
1.3.1 Stepping Motor Operation
Stepping Motor OperationStepping Motor Operation
Stepping Motor Operation
Stepping motors are not as familiar to most people as the common C motor, but there are
some similarities. The C motor consists of an armature, an electromagnet mounted on a
rotating shaft, which is located inside a permanent magnet. Current is supplied to the
electromagnet through brushes that rub on contacts on the armature. When the
electromagnet is energized, the armature rotates to align the poles of the electromagnet with
the opposite poles of the permanent magnet. Of course, before this can occur, the rotation of
the armature changes the contact plates rubbing on the brushes so that the current is
reversed. This causes the poles of the electromagnet to reverse, establishing a force for
continued rotation. This switching action is called commutation.
In stepping motors, the rotating element, called a rotor, is generally a permanent magnet
while the fixed element, the stator, is the electromagnet. The key difference between
stepping motors and C motors, however, is the method of commutation. The C motor
commutates automatically as it rotates. Thus, the timing of the commutation is determined
by the speed of rotation, which may vary with the load or applied power. The commutation of
the stepping motor is set by external electronics, forcing the motor to rotate at a
predetermined rate. If the load is such that the motor does not have the force to produce the
correct rate of rotation, the rotation will become erratic and may even reverse.
The force exerted between two magnet poles is proportional to the square of the distance
between the poles. A motor with a single electromagnet and only two poles would exhibit
considerable loss of power when the distance between the poles of the permanent magnet
and the electromagnet was greatest. It is understandable that, in most practical C motors,
the armature has more than two poles. This allows the commutation to occur over a smaller
angle of rotation, so that the active poles can always be relatively close to the poles of the
permanent magnet.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
6
Stepping motors are also made with multiple poles on both the rotor and stator the exact
arrangement determines the number of steps per revolution. The motor used in the Lambda
10-2 has 200 steps per revolution (1.8 degrees per step). There are usually two windings in
the stator, and a single step of rotation is produced by reversing the current on one of the
windings. Reversing the current on the second winding will then produce another step. If the
first winding is then reversed again, returning to its original value, a third step will result.
Finally, reversing the second winding, so that both windings are back to their original state,
will produce a fourth step. This pattern may then be repeated to continue rotation in the
same direction. Reversing the sequence produces steps of rotation in the opposite direction.
The rate and distance of rotation is determined by the rate and number of commutation
steps. As long as the current is held constant in both windings, the rotor will not rotate. This
makes the stepping motor ideal for producing fast start and stop movements. Some
limitations should be considered. Given that there are only 4 states of the control electronics
(2 polarities for each of the 2 windings) but 200 steps per revolution, it follows that, for each
of these four states, there are 50 possible rotary positions. In order to establish the absolute
position an external sensor must be added. Absolute position only needs to be determined
once so long as the subsequent moves occur without errors.
Correct operation of stepping motors, which is outlined above, is not always obtained. If the
motor lacks the torque to accelerate and decelerate the load at the rate indicated by the
control electronics, the resulting move may differ from that commanded by the control
electronics. For instance, if enough external force is applied, the motor shaft may be rotated
even though the command signal has not changed. For small rotations (less than 3.6
degrees), the motor shaft will return to the correct position if the force is removed. For
larger rotations, the shaft will stop at the nearest of the 50 correct positions for that
command signal when the external force is removed.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
7
2.
2.2.
2. INSTALLATION
INSTALLATIONINSTALLATION
INSTALLATION
2.1
2.12.1
2.1 Unpacking
UnpackingUnpacking
Unpacking
The Lambda 10-2 and associated hardware comes packed in a single carton. The following is
a list of the components found there. If two wheels were ordered, two of everything should
have been received (except those items marked with an asterisk (*)). If you believe that any
of these components are missing or show obvious signs of damage from shipping, please
contact the factory.
1 or 2 Filter Wheels, each a 10 position 25 or 32 mm, or 5
1 or 2 Filter Wheels, each a 10 position 25 or 32 mm, or 51 or 2 Filter Wheels, each a 10 position 25 or 32 mm, or 5
1 or 2 Filter Wheels, each a 10 position 25 or 32 mm, or 5-
--
-position 50 mm, and each
position 50 mm, and each position 50 mm, and each
position 50 mm, and each
with or without a shutter (25 or 35 mm
with or without a shutter (25 or 35 mmwith or without a shutter (25 or 35 mm
with or without a shutter (25 or 35 mm
Lambda 10
Lambda 10Lambda 10
Lambda 10-
--
-2 Controller*
2 Controller*2 Controller*
2 Controller*
Support Base per filter wheel
Support Base per filter wheelSupport Base per filter wheel
Support Base per filter wheel
2 Support Rods p
2 Support Rods p2 Support Rods p
2 Support Rods per filter wheel
er filter wheeler filter wheel
er filter wheel
2 Base Clamps per filter wheel
2 Base Clamps per filter wheel2 Base Clamps per filter wheel
2 Base Clamps per filter wheel
Filter Wheel to Controller Connecting Cable (1 per filter wheel)
Filter Wheel to Controller Connecting Cable (1 per filter wheel)Filter Wheel to Controller Connecting Cable (1 per filter wheel)
Filter Wheel to Controller Connecting Cable (1 per filter wheel)
Parallel Interface Cable*
Parallel Interface Cable*Parallel Interface Cable*
Parallel Interface Cable*
Serial Interface Cable*
Serial Interface Cable*Serial Interface Cable*
Serial Interface Cable*
Power Cord*
Power Cord*Power Cord*
Power Cord*
2 Individual Filter Holders (per filter wheel)
2 Individual Filter Holders (per filter wheel)2 Individual Filter Holders (per filter wheel)
2 Individual Filter Holders (per filter wheel)
Retaining Ring river*
Retaining Ring river*Retaining Ring river*
Retaining Ring river*
Manual*
Manual*Manual*
Manual*
Micr
MicrMicr
Microscope Adapters (if ordered)
oscope Adapters (if ordered)oscope Adapters (if ordered)
oscope Adapters (if ordered)
Each 10-position filter wheel is shipped with nine positions of the wheel containing retaining
rings, spacer rings and blanking discs. One of the individual filter holders contains the same
three components. The second individual filter holder and one position of the wheel (position
#1) contain only retainers and spacers.
The Lambda 10-2 is shipped to you in a prefabricated foam mold. Please take note of this
method of packaging. Should it ever be necessary to ship the puller to another location, the
same method of packaging should be employed. Additional packing material may be
purchased from Sutter Instruments.
IMPORTANT: Improper packaging is a form of abuse and, as such, can be responsible for
voiding the warranty where shipping damage is sustained because of such packing.
2.2
2.22.2
2.2 Pre
PrePre
Pre-
--
-installation Considerations
installation Considerationsinstallation Considerations
installation Considerations
2.2.1
2.2.12.2.1
2.2.1 Vibrations
VibrationsVibrations
Vibrations
The Lambda 10-2 filter wheel system was designed to produce fast filter changes with a
minimum of vibration. The microprocessor controls the trajectory of each move using
microsteps with programmed acceleration and deceleration profiles. The result is a system so
smooth that customers using the Lambda 10 for the first time have called to report that
their wheel isn’t turning simply because they could not hear or feel anything.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
8
Although the Lambda 10-2
is smooth and quiet, even the slightest vibration can produce
problems for the electrophysiologist. Vibration can come from several sources. The most
pronounced effects normally come from the high-speed shutter, if that option has been
included. The shutter produces a very sharp impulse which is best avoided by finding
another way of turning the light on and off such as turning to a blanked filter position.
When the motor accelerates and decelerates the filter wheel rapidly, the torque applied tends
to turn the motor and the Lambda housing in the opposite direction. If the housing is not
firmly attached, this may appear as vibration that increases with the selected speed and the
weight of filters loaded. This is not usually a problem if the wheel is firmly mounted to a
good solid surface.
Stepping motor-based systems can produce considerable vibration and noise if the rate at
which steps are taken excites a system resonance. This problem is greatly reduced by
reducing the step size with microstepping, as in the Lambda 10-2. Even when microstepping
is used, it is possible to produce vibration by selecting a speed that is not optimal or by
starting a new move within a few milliseconds of the end of the previous move. For example,
with only two typical filters loaded speed 1 should give a very smooth move that stops with
little vibration. Speed 2, 3, and 7 may all produce more vibration at the end of a move with
this load even though they are slower speeds.
At a minimum, we recommend that the Lambda 10-2 should be firmly mounted to a solid
surface by the support stand, which is included with each unit. o not depend on the optical
coupling between the microscope and the Lambda 10 to support and stabilize the Lambda
10. This configuration will be adequate in many cases. If there is still too much vibration, the
wheel may be mounted using the support stand, but with an air gap between the Lambda 10
and the microscope.
The best cure for vibration is isolation. One simple approach is to mount the filter wheel to a
wall or a separate table. If the microscope is on an air table, be aware that the microscope
might drift relative to a filter wheel mounted on a separate, stable surface. In such cases, it
may be better to couple the Lambda 10 to the microscope with a flexible light guide. Contact
Sutter Instrument for detail of our light-guide adapter system.
2.3
2.32.3
2.3 Installing the Controller
Installing the ControllerInstalling the Controller
Installing the Controller
2.3.1
2.3.12.3.1
2.3.1 Line Po
Line PoLine Po
Line Power (Mains)
wer (Mains)wer (Mains)
wer (Mains)
The power cord provided with the Lambda 10-2 connects to the Power Entry Module located
on the back of the unit (see Figure 2-1). This Module also includes the Line Fuse and Voltage
Selector Switch.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
9
Figure 2-1. Lambda 10-2 Cabinet (rear view).
Confirm that the Voltage Selector Switch on the Power Entry Module is set to the proper
value (110V a.c. or 220V a.c.). If it is not, turn the selector switch until the appropriate value
is lined up with the indicator. You must also replace the fuse with the appropriate value (see
the Technical Specifications), otherwise your protection from fire and electric shock may be
compromised.
Make certain that the ON/OFF Switch located on the front panel of the Lambda 10-2 cabinet
is turned OFF.
Figure 2-2. Lambda 10-2 Cabinet (front view)
Plug the power cord provided with the Lambda 10-2 into the Line Input socket on the Power
Entry Module and then to a power source of the appropriate voltage and frequency.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
10
Figure 2-3. Power connection
2.4
2.42.4
2.4 Installing the Filter Wheel
Installing the Filter WheelInstalling the Filter Wheel
Installing the Filter Wheel
If you have not yet mounted the filter wheel on the support hardware, please refer to the
FIRST TIME USE chapter of this manual for instructions. Now move the mounted filter
wheel to the approximate final location and adjust the filter wheel to the desired height. The
filter wheel can be mounted on the posts in either orientation (optical port up or down), as
required. The filter wheel can now be interfaced with other instruments (e.g. a microscope
and a light source) using an appropriate adapter (Figure 2-4). If you need to purchase an
adapter or require a custom adapter for your experimental setup, please contact the
Technical Support staff at Sutter Instrument Company by phone (415 883-0128) or by E-
mail (info@sutter.com).
Figure 2-4. Schematic diagram of two possible experimental configurations
The optical ports in the filter wheel housing and cover plate are threaded 1-32 (standard C-
mount) allowing direct connection of like-threaded devices (camera adapters, microscope
adapters, etc.)
CAUTION
CAUTION CAUTION
CAUTION: Care should be taken that any devices threaded into the ports
: Care should be taken that any devices threaded into the ports : Care should be taken that any devices threaded into the ports
: Care should be taken that any devices threaded into the ports not
notnot
not
project more than 0.180 inches into the port. Allow
project more than 0.180 inches into the port. Allowproject more than 0.180 inches into the port. Allow
project more than 0.180 inches into the port. Allowing threaded devices to penetrate
ing threaded devices to penetrate ing threaded devices to penetrate
ing threaded devices to penetrate
further could result in damage to the filter wheel or filter cups.
further could result in damage to the filter wheel or filter cups. further could result in damage to the filter wheel or filter cups.
further could result in damage to the filter wheel or filter cups.
Once the filter wheel has been attached to the other instrument(s), tighten the wing screws
to lock the filter wheel at its final height. It is intended that the filter wheel be supported on
the two posts included with your system. These posts can be screwed into the base provided
or into any surface with ¼”-20 holes drilled on 6” centers at an appropriate position on the

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
11
tabletop or optical bench. When using the support rod base always secure it to the tabletop
using the toe clamps provided with the system.
If a heavy light source is to be used (such as a vapor lamp with integral igniter) it should
have its own separate support system.
If your filter wheel has a shutter, the side of the filter wheel on which the shutter is installed
(the “motor side” as shown in Figure 2-4) would typically be mounted toward the light
source. Placing the shutter between the lamp and filters limits the amount of heat and light
to which the filters are exposed and increases the filters’ useful lifetime.
2.4.1
2.4.12.4.1
2.4.1 Filter Wheel Assembly Set Up
Filter Wheel Assembly Set UpFilter Wheel Assembly Set Up
Filter Wheel Assembly Set Up
1. Assemble the two Support Rods to the Support Base. Flats have been machined near the
threaded end of these rods to facilitate tightening with a wrench. The pair of support
base clamps should be used to anchor the base to some convenient location for testing.
These clamps are designed to allow anchoring to any of the popular hole-patterns found
in optical benches.
2. Slide the filter wheel down onto the support rods and lock it at a convenient height using
the wing screws on the mounting ears of the filter wheel.
Figure 2-5. Filter wheel mounting stand
3. Connect the controller and filter wheel(s) with the appropriate cable(s) provided. It is
important that the screws anchoring the cable ends be tight to ensure a good connection
for the motor power leads and positioning signals.

LAMBDA 10-2 OPERATION MANUAL – REV. 2.04 (20120224)
12
Figure 2-6. Filter wheel connection
CAUTION:
CAUTION: CAUTION:
CAUTION: O NOT CONNECT OR ISCONNECT THE CABLES BETWEEN
O NOT CONNECT OR ISCONNECT THE CABLES BETWEEN O NOT CONNECT OR ISCONNECT THE CABLES BETWEEN
O NOT CONNECT OR ISCONNECT THE CABLES BETWEEN
THE CONTROLLER AN THE MECHANICAL UNITS (FILTER WHEELS AN /OR
THE CONTROLLER AN THE MECHANICAL UNITS (FILTER WHEELS AN /OR THE CONTROLLER AN THE MECHANICAL UNITS (FILTER WHEELS AN /OR
THE CONTROLLER AN THE MECHANICAL UNITS (FILTER WHEELS AN /OR
SHUTTER
SHUTTERSHUTTER
SHUTTERS) WHILE POWER IS ON.
S) WHILE POWER IS ON. S) WHILE POWER IS ON.
S) WHILE POWER IS ON.
Please allow at least 20 seconds after turning the unit off before disconnecting the
Please allow at least 20 seconds after turning the unit off before disconnecting the Please allow at least 20 seconds after turning the unit off before disconnecting the
Please allow at least 20 seconds after turning the unit off before disconnecting the
mechanical units. Failure to do this may result in damage to the electronics.
mechanical units. Failure to do this may result in damage to the electronics.mechanical units. Failure to do this may result in damage to the electronics.
mechanical units. Failure to do this may result in damage to the electronics.
2.5
2.52.5
2.5 First Time Use
First Time UseFirst Time Use
First Time Use
Make certain that the Lambda 10-2 controller is properly connected to the mains power
(refer to Section 2.3.1 and to Figure 2-1 and Figure 2-2).
2.5.1
2.5.12.5.1
2.5.1 Make It Go
Make It GoMake It Go
Make It Go
1. Turn on the power using the ON/OFF switch on the front panel of the Lambda 10-2
controller cabinet (see Figure 2-2). The controller will briefly display an initialization
screen and the filter wheel will automatically go to the “Home” (0) filter position. The
current filter position will appear on the controller front panel display.
Figure 2-7. Initialization screen.
2. The Lambda 10-2 will initially be set to respond to the parallel port as its input source.
To manually select a filter you must first press the LOCAL key on the keypad. The
display will now indicate that the MANUAL (keypad) input source is active.
FILTER-A # 0 SP. 2 B NOT CONNECTED
PARALLEL SHUTTER A-OFF B-OFF
Filter position currently
selected on Filter Wheel A
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