Teledyne AutoInjector Module Operating instructions

Automation Modules
Installation and Operation Guide
69-5233-772
Copyright © 2016 Teledyne Technologies Incorporated. All rights reserved.
Released: July 2016

Automation Modules Installation and Operation Guide
Safety Warnings
3
Automation Mo dules Installat ion and Operat ion Guide
Safety Warnings
Safety Before installing, operating, or maintaining this equipment, it is
imperative that all hazards and preventive measures are fully
understood. While specific hazards may vary according to
location and application, take heed in the following general
warnings:
WARNINGWARNING
Liquids associated with this instrument may be classified
as carcinogenic, biohazard, flammable, or radioactive.
Should these liquids be used, it is highly recommended that
this application be accomplished in an isolated
environment designed for these types of materials in
accordance with federal, state, and local regulatory laws,
and in compliance with your company’s chemical/hygiene
plan in the event of a spill.
WARNINGWARNING
Avoid hazardous practices! If you use this instrument in
any way not specified in this manual, the protection
provided by the instrument may be impaired.
WARNINGWARNING
If you are using flammable solvents or chemicals with this
system, vapor concentration levels may exceed the
maximum exposure levels as recommended by OSHA
Guide 1910.1000. To reduce those levels to a safe exposure,
Teledyne Isco recommends that you place the system in a
laboratory hood designed for the purpose of ventilation.
This hood should be constructed and operated in
accordance with federal state and local regulations. In the
event of a solvent or chemical spill, your organization
should have a plan to deal with these mishaps. In all cases,
use good laboratory practices and standard safety
procedures.
Hazard Severity Levels This manual applies Hazard Severity Levels to the safety alerts.
These three levels are described in the sample alerts below.
CAUTION
Cautions identify a potential hazard, which if not avoided, may
result in minor or moderate injury. This category can also warn
you of unsafe practices, or conditions that may cause property
damage.
WARNINGWARNING
Warnings identify a potentially hazardous condition, which
if not avoided, could result in death or serious injury.

Automation Modules Installation and Operation Guide
Safety Warnings
4
DANGER
DANGER – limited to the most extreme situations
to identify an imminent hazard, which if not
avoided, will result in death or serious injury.
Hazard Symbols The equipment and this manual use symbols used to warn of
hazards. The symbols are explained in the table below.
Hazard Symbols
Warnings and Cautions
The exclamation point within the triangle is a
warning sign alerting you of important instructions
in the instrument’s technical reference manual.
The lightning flash and arrowhead within the trian-
gle is a warning sign alerting you of “dangerous
voltage” inside the product.
Symboles de sécurité
Ce symbole signale l’existence d’instructions
importantes relatives au produit dans ce manuel.
Ce symbole signale la présence d’un danger
d’électocution.
Warnungen und Vorsichtshinweise
Das Ausrufezeichen in Dreieck ist ein Warn-
zeichen, das Sie darauf aufmerksam macht, daß
wichtige Anleitungen zu diesem Handbuch
gehören.
Der gepfeilte Blitz im Dreieck ist ein Warnzeichen,
das Sei vor “gefährlichen Spannungen” im
Inneren des Produkts warnt.
Advertencias y Precauciones
Esta señal le advierte sobre la importancia de las
instrucciones del manual que acompañan a este
producto.
Esta señal alerta sobre la presencia de alto
voltaje en el interior del producto.

Automation Modules Installation and Operation Guide
Safety Warnings
5
For Additional
Information
Technical assistance for the Teledyne Isco Automation Modules
can be obtained from:
Teledyne Isco
4700 Superior St.
Lincoln NE 68504
Phone: [phone] or [phone]
Fax: [phone]
E-mail: [e-mail]

Automation Modules Installation and Operation Guide
Safety Warnings
6

7
Automation Modules
Table of Contents
Section 1 Introduction
1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Section 2 The AutoInjector Module
2.1 AutoInjector Module Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2 AutoInjector Module Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
2.2.1 Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8
2.2.2 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-9
Section 3 The AutoSampler Module
3.1 AutoSampler Module Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.1.1 Installing the AutoSampler Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.1.2 Establishing Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
3.2 Installing the AutoSampler Module with the Column Selector Module . . . . . . . 3-19
3.3 AutoSampler Module Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-20
3.3.1 Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-20
3.3.2 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-21
Section 4 The Column Selector Module
4.1 Column Selector Module Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.1 Installing the Column Selector Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.2 Establishing Column Selector Module Connections . . . . . . . . . . . . . . . . . . 4-6
4.1.3 Mounting the AutoInjector Module to the Column Selector Module . . . . . 4-8
4.1.4 Establishing AutoInjector Module Connections . . . . . . . . . . . . . . . . . . . . . 4-16
4.2 Installing the Column Selector Module with the AutoSampler Module . . . . . . . 4-16
4.3 Column Selector Module Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16
4.3.1 Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-16
Appendix A Specifications
A.1 AutoInjector Module Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1
A.2 AutoSampler Module Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2
A.3 Column Selector Module Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3

Automation Modules
Table of Contents
8

1-1
Automation Modules
Section 1 Introduction
1.1 Overview The AutoInjector Module, AutoSampler Module, and Column
Selector Module allow you to increase the productivity of the
CombiFlash®EZ Prep by automating a number of tasks involved
with high-performance liquid chromatography (HPLC). These
modules are controlled by Teledyne Isco's PeakTrak®software in
order to maintain the ease-of-use you are familiar with and
practically eliminate the need for training.
The three modules can be used separately or in conjunction with
one another for even more automated operation.
AutoInjector Module The AutoInjector Module performs automated, repetitive com-
pound injections completely unattended. This allows users to
automatically purify amounts of compound larger than what can
be done with a single separation run. As a result of having this
tedious process automated, user productivity is increased.
More information regarding the AutoInjector Module including
installation, setup, and operation can be found in Section 2.
AutoSampler Module The AutoSampler Module performs automated purifications on
multiple samples. In addition, the module doubles the fraction
collection capacity of the EZ Prep by adding two collection racks.
For more flexibility, samples can consist of multiple injections.
You can even choose between maximizing rack capacity by filling
racks completely or optimizing work flow on a shared system by
having each sample sent to the next rack. The racks utilize the
same RFID rack swapping technology of the EZ Prep, allowing
users to remove completed racks and replace them with new
racks for practically unlimited fraction collection capacity.
More information regarding the AutoSampler Module including
installation, setup, and operation can be found in Section 3.
Column Selector Module The Column Selector Module allows the EZ Prep to automati-
cally access up to four prep columns. This allows users easy
access to a variety of column sizes and chemistries. It also allows
users to dedicate columns to both high and low pH separations.
When combined with the AutoSampler Module, each sample can
be separated on the column best suited for the job.
More information regarding the Column Selector Module
including installation, setup, and operation can be found in
Section 4.

Automation Modules
Section 1 Introduction
1-2

2-1
Automation
Section 2 The AutoInjector Module
2.1 AutoInjector Module
Installation
Note
CombiFlash EZ Prep software must be version 3.1.0 or higher
to support the automation capabilities.
Note
If the AutoInjector Module is used in conjunction with the Col-
umn Selector Module, the standard prep column mount can
be removed to keep the overall system width minimized. In
this case, the AutoInjector Module can be mounted on top of
the Column Selector Module instead of on top of the standard
prep column mount.
Installation 1. Turn off the EZ Prep.
2. Remove the top cover of the EZ Prep column mount by
grasping and pulling straight up.
Figure 2-1 Removing the top cover of the EZ Prep column
mount
3. Remove the Luer fitting from the EZ Prep inject valve.

Automation
Section 2 The AutoInjector Module
2-2
Figure 2-2 Removing the Luer fitting from the EZ Prep
inject valve
4. Remove the waste line from the EZ Prep inject valve.
Remove the check valve from this line and set it aside for
later use. Discard the waste line.
Figure 2-3 Removing the waste line from the EZ Prep
inject valve
5. Install the AutoInjector Module by placing it on top of the
prep column mount and pressing downwards. The tab on
the bottom of the AutoInjector Module will snap into the
receptacle on the top of the prep column mount.

Automation
Section 2 The AutoInjector Module
2-3
Figure 2-4 AutoInjector Module attached to the EZ Prep
column mount
6. Connect the USB cable from the AutoInjector Module to
the USB port on the rear of the EZ Prep. If you have a
CombiFlash Rf+ PurIonTM Mass Detector attached to the
EZ Prep, you will need to obtain a USB hub to connect both
modules.
7. Connect the included power supply from the AutoInjector
Module to a suitable power connection. Use a power supply
in a location that minimizes the possibility of a chemical
spill damaging the power supply.
8. Locate the supplied waste tubing and fitting package (P/N
60-5234-653).
9. Unscrew the attached plastic ferrule in this fitting package
until the plastic ferrule is almost completely detached from
the steel fitting.
10. Attach the steel fitting in this package to the EZ Prep
inject valve waste port. This steel fitting should be hand
tight.

Automation
Section 2 The AutoInjector Module
2-4
Figure 2-5 Waste tubing fitting attached to the EZ Prep
inject valve
11. Turn the plastic ferrule until it is attached hand tight to
the valve.
12. Turn the steel fitting 1/2turn tighter.
13. Remove the plastic ferrule while leaving the fitting
attached.
Figure 2-6 Waste fitting with the plastic ferrule removed
14. Attach the supplied tubing from this package to the
secured fitting. Tighten the ferrule finger tight with the
supplied tool. Ensure the tubing and fitting are snug in the
EZ Prep inject valve.

Automation
Section 2 The AutoInjector Module
2-5
Figure 2-7 Waste fitting with tubing attached
15. Connect the opposite end of this tubing to the left port of
the AutoInjector Module.
Figure 2-8 Connection of the waste tubing from the EZ
Prep inject valve to the left AutoInjector
Module port
16. Connect the supplied waste line (P/N60-5234-654) to the
right port of the AutoInjector Module.

Automation
Section 2 The AutoInjector Module
2-6
Figure 2-9 Waste line connected to the right AutoInjector
Module port
17. Connect the check valve removed in Step 4 to the waste
line from the AutoInjector Module. Ensure that the arrow
on the check valve points away from the AutoInjector Mod-
ule as shown in Figure 2-10.
Figure 2-10 Check valve connected to the waste line of the
AutoInjector Module
18. Locate the supplied sample probe tubing and fitting pack-
age (P/N 60-5234-657).
19. Unscrew the attached plastic ferrule in this fitting package
until the plastic ferrule is almost completely detached from
the steel fitting.
20. Attach the steel fitting in this package to the EZ Prep
inject valve waste port. This steel fitting should be hand
tight.

Automation
Section 2 The AutoInjector Module
2-7
Figure 2-11 Sample tube fitting attached to the EZ Prep
inject valve
21. Turn the plastic ferrule until it is attached hand tight to
the valve.
22. Turn the steel fitting 1/2turn tighter.
23. Remove the plastic ferrule while leaving the fitting
attached.
Figure 2-12 Sample tube fitting with the plastic ferrule
removed
24. Attach the supplied tubing from this package to the
secured fitting by screwing the new plastic ferrule into the
fitting. Tighten the ferrule finger tight with the supplied
tool.

Automation
Section 2 The AutoInjector Module
2-8
Note
The spring attached to the tubing prevents kinks from develop-
ing in the tubing.
Figure 2-13Sample tube fitting with sample tubing
attached
25. Turn on the EZ Prep, or cycle power if the EZ Prep is
already turned on.
26. Go to TOOLS | CONFIG | PREP HPLC, and select the loop
size used on the EZ Prep. This allows the software to
ensure the loop doesn’t get overfilled when separating
large samples. The software will display a warning if the
system is programmed to fill the loop to more than 50% of
total volume. This warning can be ignored at the discretion
of the user.
2.2 AutoInjector Module
Operation
2.2.1 Setup Before using the AutoInjector Module for the first time, the EZ
Prep should be configured for the proper injection loop volume.
1. Begin by going to TOOLS | CONFIG | PREP HPLC.
2. Select the loop volume field as shown in Figure 2-14.
Note
The screen shot in Figure 2-14 depicts what is shown when a
Column Selector Module is installed. If the Column Selector
Module is not installed, this screen may appear differently.

Automation
Section 2 The AutoInjector Module
2-9
Figure 2-14 Configuring the injection loop volume
For best results, the loop should generally be 50% filled for
maximum compound recovery. If the parameters entered for the
individual injection exceed a 50% loop fill, a warning message
will appear recommending a change in the parameters. This
warning can be ignored at the discretion of the user.
2.2.2 Operation To use the AutoInjector Module, follow the steps below:
1. Place the sample probe into your sample container.
2. Press PLAY to start a separation as you would for an indi-
vidual separation.
3. If you want to automatically perform multiple injections
(or perform a single injection using the AutoInjector Mod-
ule), select the “Multiple Injections” drop down as shown in
Figure 2-15. This is the default selection if an AutoInjector
Module is present.
4. If you want to manually inject your sample without using
the AutoInjector Module, select “Load Now” or “Load After
Equilibrium”. When prompted to load the sample, remove
the sample probe line from the inject valve and inject
directly into the valve.

Automation
Section 2 The AutoInjector Module
2-10
Figure 2-15 Selecting for multiple injections
5. In the dialogue box, enter the total sample volume and the
number of injections as show in Figure 2-16.
Figure 2-16 Selecting the sample volume and number of
injections