Teledyne CombiFlash Torrent User manual

CombiFlash
®
Torrent™
Quick Start Guide
Overview
These instructions supplement the
CombiFlash Torrent Module Installation Guide (part
number 60-5243-135) and briefly describe the steps nec-
essary to perform a purification run. These quick start
instructions assume that:
●the system has been installed according to sec-
tion 2 of the Installation Guide,
●the operator is familiar with the safety warnings
in section 1 of the Installation Guide, and
●the system will be operated from the touch panel
display.
Quick Start Instructions
1. Perform Thin Layer Chromatography (TLC) on
the sample. From the TLC you'll learn of a suit-
able column media and solvent system, and the
difficulty of the compound separation.
2. With the column media known, select a
RediSep®Rf column size appropriate for your
intended sample load. Refer to the Help topic
“RediSep Rf Column Selection Guide” for recom-
mended sample load ranges. If the separation is
difficult (Rf<0.2), your sample load should be
near the low end of the selected column’s range.
3. Insert the RediSep Rf column on the
CombiFlash Torrent module. PeakTrak software
loads a method for that column. You can manu-
ally select the column media and size if the sys-
tem does not detect the column.
Note
Raising the upper column mount assembly from its
top- or bottom-most point of travel triggers the RFID
column detection. If you exchange a column without
fully lowering or raising the upper column mount,
select the File>New menu command instead to detect
the RediSep Rf column.
4. Ensure that the Solvent A and Solvent B lines are
routed to suitable solvents.
5. Select Tools>Auto Prime to open the Auto Prime
Window (Figure 1). Click the Play button. The
system primes the internal tubing and then
closes the Auto Prime window.
Figure 1: PeakTrak’s Auto Prime window
6. On the main window, click the Play button.
7. From the Run Requirements window, select a
Loading Type.
❍If the sample is soluble in the starting mobile
phase (Solvent A), select Liquid.
❍If the sample is not soluble in the starting
mobile phase, select Solid (Pause).
❍If the sample is loaded directly on the column
media, select None. The separation will begin
immediately without equilibration when you
click the OK button.
Note
Use the Solid option only when the solid sample
cartridge is already prepared and loaded on the
module. The module will equilibrate the column and
then automatically begin the run without user
intervention.
Instruction Sheet 69-5243-134
February 12, 2010

Instruction Sheet 69-5243-134 February 12, 2010
Teledyne Isco, Inc.
P.O. Box 82531, Lincoln, Nebraska, 68501 USA
Toll-free: [phone] ˘ Phone: [phone] ˘ Fax: [phone]
E-mail: [email protected]
8. Click OK to start the run.
If you selected Liquid or Solid (Pause), continue with
step 9.
If you selected None, skip steps 9 through 11 and
continue with step 12.
9. The system will equilibrate the column. During
this time, prepare the sample.
❍If you selected Liquid, dissolve the sample in a
minimal volume of Solvent A (or other misci-
ble solvent) and draw the liquid into a
syringe. Alternatively, prepare the optional
Sample Load Pump.
❍If you selected Solid (Pause), prepare the sample
using a solid sample cartridge. Refer to the
Help topic “Preparing Solid Samples” for
instructions.
Figure 2: Pause messages
10. The CombiFlash Torrent module will pause after
performing the column equilibration (Figure 2).
It will ask you to inject the sample or load the
solid sample cartridge.
If Liquid – inject or pump the liquid into the
sample injection port. To force the sample
through the valve, follow this injection with
another 5 mL of the starting mobile phase
(chase solvent).
If Solid – load the cartridge on the
CombiFlash Torrent module. To load the car-
tridge:
a. The solid load cartridge cap must match the
size of your selected cartridge. If it does not
match, install the correct cap now.
b. Press the lever on the side of the cap and fully
extend the plunger.
c. Slide the plunger into the cartridge until it
reaches the top frit.
d. Press the lever and push the cartridge into
the cap. Align the cartridge so that it fits fully
into the recess in the cap.
e. Rotate the cartridge 1/4-turn to secure it in the
cap. For 25 gram cartridges, rotate the car-
tridge clockwise. For cartridge sizes 65 gram
and larger, rotate the cartridge counterclock-
wise.
Note
Refer to the instruction sheet titled “Adjustable Rf
Solid Load Cartridge Cap for RediSep Rf” (available at
www.isco.com) for complete instructions.
11. Click OK to begin the run.
12. Monitor the red absorbance trace.
The CombiFlash Torrent module will proceed
through the run and collect fluids in the fraction
tubes. It isolates peak fluids from non-peak flu-
ids. Tube change marks on the screen and the
tube map help you locate your compounds.
For detailed operating instructions, refer to the
on-line help.
Operation Tip: Difficult Separations — If the TLC indi-
cates a difficult separation (Rf<0.2), click the Pause
button at onset of each peak in the absorbance trace.
This inserts an isocratic hold and extends the run. The
isocratic hold can increase the resolution by delaying
the release of the next closely eluting peak. When the
absorbance of the peak is close to the baseline again,
click the Pause button to resume the linear gradient
which will help to maintain sharp peaks.
Assistance Available
Should you need assistance with these instructions,
please contact Teledyne Isco.
Liquid Samples
Solid Samples