Thermo Forma Cryomed 1010 Operating instructions

Cryomed
Model 1010/1011
Programmable Freezing System
Operating and Maintenance Manual
Manual No: 7001010 Rev. 3

Model 1010 and 1011_______________________________________________________________________________
i
Read This Instruction Manual.
Failure to read, understand and follow the instructions in
this manual may result in damage to the unit, injury to operat-
ing personnel, and poor equipment performance.
CAUTION! All internal adjustments and maintenance must
be performed by qualified service personnel.
Refer to the serial tag on the back of this manual.
The material in this manual is for information purposes
only. The contents and the product it describes are subject to
change without notice. Thermo Forma makes no representations
or warranties with respect to this manual. In no event shall
Thermo Forma be held liable for any damages, direct or inciden-
tal, arising out of or related to the use of this manual.
MANUAL NUMBER 7001010
3 -- 2/5/01 Updated format to Quark ccs
2 19303/FR-1913 6/14/00 Removed calibration section ccs
-- 16847 10/3/97 Corrected error setpoint limits heg
-- FR-1094 3/26/97 Added printer cable and DIP settings heg
1 FR-167 12/13/96 Revised calibration procedures heg
0 -- 8/93 Original manual
REV ECR/ECN DATE DESCRIPTION By

Model 1010 and 1011_________________________________________________________________________Warnings
ii
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Ce symbole attire l'attention de l'utilisateur sur des instructions importantes de fonctionnement et/ou d'entretien. Il
peut être utilisé seul ou avec d'autres symboles de sécurité. Lire attentivement le texte d'accompagnement.
Wichtige Betriebs- und/oder Wartungshinweise. Lesen Sie den nachfolgenden Text sorgfältig.
Importante instruccions de operacion y/o mantenimiento. Lea el texto acompanante cuidadosamente.
Potential electrical hazards. Only qualified persons should perform procedures associated with this symbol.
Ce symbole attire l'attention de l'utilisateur sur des risques électriques potentiels. Seules des personnes qualifiées
doivent appliquer les instructions et les procédures associées à ce symbole.
Gefahr von Stromschlägen. Nur qualifizierte Personen sollten die Tätigkeiten ausführen, die mit diesem Symbol beze-
ichnet sind.
Potencial de riesgos electricos. Solo personas das capacitadadas deben ejecutar los procedimientos asociadas con este
simbulo.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Risques potentiels liés à l'énergie. L'équipement en entretien ou en maintenance doit être éteint et mis sous clé pour
éviter des blessures possibles.
Geräte, an denen Wartungs- oder Servicearbeiten durchgeführt werden, müssen abgeschaltet und abgeschlossen wer-
den, um Verletzungen zu vermeiden.
El equipo recibiendo servicio o mantenimiento debe ser apagado y segurado para prevenir danos.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be damaged by elevated
temperatures.
Présence de surface(s) chaude(s) pouvant causer des brûlures sur la peau non protégée, ou sur des matières pouvant
être endommagées par des températures élevées.
Heiße Oberfläche(n) können ungeschützter Haut Verbrennungen zufügen oder Schäden an Materialien verursachen,
die nicht hitzebeständig sind.
Superficias calientes que pueden causar quemaduras a piel sin proteccion o a materiales que pueden estar danados
por elevadas temperaturas.
√ Always use the proper protective equipment (clothing, gloves, goggles, etc.)
√ Always dissipate extreme cold or heat and wear protective clothing.
√ Always follow good hygiene practices.
√ Each individual is responsible for his or her own safety.

Models 1010 and 1011__________________________________________________________________________Safety
iii

Models 1010 and 1011___________________________________________________________________Table of Contents
iv
Table of Contents
Section 1 - Receiving . . . . . . . . . . . . . . . . . . . . . . . . . .2 - 1
1.1 Preliminary Inspection . . . . . . . . . . . . . . . . . . . . . .2 - 1
1.2 Visible Loss or Damage . . . . . . . . . . . . . . . . . . . . .2 - 1
1.3 Responsibility for Shipping Damage . . . . . . . . . . .2 - 1
Section 2 - Introduction . . . . . . . . . . . . . . . . . . . . . . . .2 - 1
2.1 Standard Features . . . . . . . . . . . . . . . . . . . . . . . . . .2 - 1
a. Model 8018 Programmable Controller . . . . . . . . .2 - 1
b. Countertop Freezing Chamber . . . . . . . . . . . . . . .2 - 2
c. Strip Chart Temperature Recorder . . . . . . . . . . . .2 - 2
e. DIP Switch Settings . . . . . . . . . . . . . . . . . . . . . . .2 - 2
f. Printer Interface Cable . . . . . . . . . . . . . . . . . . . . .2 - 2
Section 3 - Installation . . . . . . . . . . . . . . . . . . . . . . . .3 - 1
3.1 Unpacking List . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 - 1
3.2 Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 - 1
3.3 Electrical Connections . . . . . . . . . . . . . . . . . . . . . . .3 - 1
3.4 Connecting the Liquid Nitrogen Transfer Hose . . . .3 - 2
Section 4 - Operation . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 1
4.1 Control Panel Operation . . . . . . . . . . . . . . . . . . . . .4 - 1
a. Control Section . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 1
b. Mode Select . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 1
c. System Status . . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 2
d. TC Scanner . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 2
e. Program Display . . . . . . . . . . . . . . . . . . . . . . . . .4 - 2
f. Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 2
g. Prog. Adv. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 3
h. Numerical and Special Functions . . . . . . . . . . . . .4 - 3
4.3 System Operation . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 3
4.4 Initiating a Freezing Run . . . . . . . . . . . . . . . . . . . . .4 - 4
4.5 factory Pre-set Programs . . . . . . . . . . . . . . . . . . . . .4 - 4
4.6 Programming the Controller . . . . . . . . . . . . . . . . . .4 - 5
4.7 Running the Program in the Unlimited Access (Manual)
Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 7
4.8 Aborting a Program Run . . . . . . . . . . . . . . . . . . . . .4 - 7
4.9 Programming the Access Code . . . . . . . . . . . . . . . .4 - 7
4.10 Programming the Error Limit Setpoints . . . . . . . .4 - 7
4.11 Programming a Sample Freezing Point Determination
Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 7
4.12 RS-232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 - 8
Section 5 - Maintenance . . . . . . . . . . . . . . . . . . . . . . . .5 - 1
5.1 Cleaning the Freezing Chamber . . . . . . . . . . . . . . . .5 - 1
5.2 Troubleshooting Chart - Model 8018 Controller . . .5 - 2
5.3 Troubleshooting Chart - Freezing Chamber . . . . . . .5 - 2
Section 6 - Specifications . . . . . . . . . . . . . . . . . . . . . . .6 - 1
Section 7 - Parts List . . . . . . . . . . . . . . . . . . . . . . . . . .7 - 1
Section 8 - Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . .8 - 1

Section 1 - Receiving
1.1 Preliminary Inspection
This item was thoroughly inspected and carefully packed
prior to shipment and all necessary precautions were taken to
ensure safe arrival. Immediately upon receipt, before the unit is
moved from the receiving area, carefully examine the shipment
for loss or damage. Unpack the shipment and inspect both inte-
rior and exterior for any in-transit damage.
1.2 Visible Loss or Damage
If any loss or damage is discovered, note any discrepancies
on the delivery receipt and call the delivering carrier and
request that their representative perform an inspection. Do not
discard any of the packing material and do not move the ship-
ment from the receiving area.
1.3 Responsibility for Shipping Damage
For products shipped F.O.B. Marietta, Ohio, the responsi-
bility of Thermo Forma ends when the merchandise is loaded
onto the carrier’s vehicle.
On F.O.B. Destination shipments, Thermo Forma’s and the
carrier’s responsibility ends when your Receiving Department
personnel sign a free and clear delivery receipt.
Whenever possible, Thermo Forma will assist in settling
claims for loss or in-transit damage.
Section 2 - Introduction
2.1 Standard Features
The Model 1010 Freezing System consists of a Model
8018 Programmable Controller, a Countertop Freezing
Chamber and a Strip Chart Temperature Recorder.
a. Model 8018 Programmable Controller
The Model 8018 is a microprocessor based programmable
controller, that provides precise automatic temperature control
for a choice of freezing chambers.
Six standard factory-set programs provide ease of operation
for common freezing operations. Additionally, nine programs
that may be operator programmed, are available to address the
user’s specific freezing applications. Each program consists of
nine sections, for continuous temperature programming. A
“jump and loop” programming feature permits the user to auto-
matically advance to subsequent steps, or return to a previous
step, as needed.
A three-digit access code provides system security, as well
as protection against inadvertent program erasure. The access
code may be used to manually override a program in “run”
mode, but on the factory-set programs the controller will not
retain in memory the value entered, and will revert back to the
factory-set program.
An audible alarm system alerts the operator of any devia-
tion from setpoint, by comparing the current chamber tempera-
ture with setpoint temperature. If the two values differ by more
than the factory-set time and temperature limits, an audible
alarm is activated, and a tracking error is logged on the strip-
chart recorder printout. The audible alarm also signals comple-
tion of the program cycle.
Note: The Tracking Error Limit setpoints were entered at the
factory for one minute, at ±5°C.
Built-in self diagnostics provide service quality assurance,
in that any test error is visually displayed in conjunction with
an audible alarm.
Models 1010 and 1011 _________________________________________________________________________Introduction
2 - 1

b. Countertop Freezing Chamber
The Model 8022, 8024 and 8026 Freezing Chamber is a
well insulated, top or front loading unit, of stainless steel con-
struction. It is sufficiently compact to be placed on a laboratory
table, yet has the capacity to house several stacked freezing
racks.
All freezing chamber space is usable. An electric blower,
mounted on the outside of the chamber, circulates gaseous
nitrogen between specimens, to assure uniform cooling.
Thermocouple temperature probes provide the programmable
freezing controller with sample, and chamber temperature infor-
mation. Keyed electrical connectors assure proper mating con-
figuration.
All chambers have a heating element to warm the freezing
chamber to +25°C, after the specimen has been removed, at the
completion of each freezing run.
Gaseous nitrogen used in the cooling process, escapes from
the freezing chamber to the atmosphere through a vent, located
at the rear of the chamber. A liquid nitrogen transfer hose and
pressure relief, set at 60 PSIG, connect to the solenoid valve to
supply coolant to the freezing chamber. Electrical connectors,
consisting of thermocouple signal leads, and control lines for
the electric blower, heater, and solenoid valve(s) are supplied.
c. Strip Chart Temperature Recorder
The LINSEIS flatbed recorders are x=f(t) recorders that
operate according to the compensation principle.
The recording follows the formula x=f(t) in a rectangular
coordinate system, the pen moves in an x-direction compensat-
ing for the supplied measurement voltage.
The resulting diagram represents an accurate recording of
the measured signal during the measured time.
The paper is moved in a vertical y-direction by a crystal
controlled stepper motor.
The mechanical construction of the flatbed recorders is
based on modules. Thus, amplifiers, stepper motor, printed cir-
cuit assembly and input modules are easy to add and easy to
change.
The printer is configured for: 0-10 Volt scale (fixed)
Chart Speed: 1/2” per minute (fixed)
The solid mechanical construction allows the use of this
recorder for all applications in scientific measurement. This
instrument can also be used for measuring and recording in
research and development, and process applications.
d. Thermal Printer (Optional)
The DPU-411 is a thermal serial dot printer unit, for print-
ing character or graphic data, from computers and other equip-
ment. The printer has eight international character sets and han-
dles many types of printing. The printing types include 40
columns of ordinary characters, 80 columns of condensed char-
acters, 20 columns of double-width characters, bold, and dou-
ble-strike characters.
Bi-directional logical-seeking increases the printer’s speed;
and the printer features a built-in Ni-cad battery that enables the
printing of approximately 1500 character lines, without connec-
tion to the AC adapter. Centronics 8-bit parallel or RS-232 seri-
al interface is an available feature.
The following accessories are included with each printer:
1. A thermal paper roll (loaded in the printer)
2. An AC adapter
3. An instruction manual
e. DIP Switch Settings
DIP Switch #1 DIP Switch #2
SW 1 OFF SW1 ON
SW2 OFF SW2 ON
SW3 ON SW3 ON
SW4 ON SW4 OFF
SW5 ON SW5 OFF
SW6 OFF SW6 OFF
SW7 ON
SW8 ON
f. Printer Interface Cable
Models 1010 and 1011 _________________________________________________________________________Introduction
2 - 2
red wire
jumper
SHI
WHT
RED
RED
RED
BLK
114
25
13
SHI
WHT
RED
BLK
114
25
13
1010
PRINTER
3-conductor cable
10 ft. in length
1010/4000565 Printer Interface Cable
Figure 2-1

Section 3 - Installation
3.1 Unpacking List
ITEMS SUPPLIED
The Model 1010 Freezing System consists of a controller,
freezing chamber and recorder. All other components required
for normal operation of the unit are supplied and include the
following:
1. Four foot nitrogen transfer hose with swivel end and fit-
tings
Note: A 6’ long hose with swivel is available as an option. The
use of a transfer hose longer than six feet is not recommended
and may degrade system performance. Contact Thermo Forma
if extended transfer hose length is required.
2. Chart paper and recorder pen
3. Power cords
4. Sample thermocouple
Note: The chamber thermocouple has been permanently
mounted behind the fan guard to assure accurate chamber tem-
perature output to the controller.
5. Stabilizing corks for securing the sample thermocouple
inside the control ampule.
REQUIRED BUT NOT SUPPLIED
In addition to the above furnished items, a low pressure
(22 PSI) liquid nitrogen supply is required.
3.2 Location
The Model 1010 Freezing System should be located in a
well ventilated area of the laboratory, on a bench top with ade-
quate work space available. Allow for adequate clearance of
chamber door swing and door latch, since such precautions are
essential to safe specimen handling.
The chamber should be located adjacent to the liquid nitro-
gen supply, and a suitable electrical outlet. If a nitrogen supply
tank is to be used, space must be allocated for tank replace-
ment.
Caution! The freezing chamber must not be posi-
tioned above the controller or recorder. Such
positioning will result in condensate dripping into
the electronics and will cause serious damage to
the internal circuitry.
Caution! The gaseous nitrogen vent, located at
the rear of the chamber must not be obstructed.
obstruction of the vent will seriously interfere
with normal system operation.
Caution! The area surrounding the gaseous
nitrogen vent is subject to low temperatures. items
adversely affected by extreme cold should be kept
away from the vent.
Arrangements should be made to collect the condensate
from the transfer hose and at the freezing chamber exhaust.
3.3 Electrical Connections
Interconnect the controller and the strip chart recorder with
the freezing chamber.
Caution! Do not force the connectors together. If
connectors do not join freely, check the pin and
socket orientation of the plug and receptacle.
Thermocouple leads are color-coded to assure
proper mating. Failure to follow these cautions
may result in permanent connector damage.
Make the following connections:
Note: Refer to the electrical schematic, or the serial tag on the
rear of the controller, for specific electrical requirements.
1. Power to the controller: With the controller power
switch set to the “OFF” position, connect the controller
power cord to a grounded power source.
2. Chamber thermocouple input: Provides chamber thermo-
couple signal to controller from freezing chamber.
Models 1010 and 1011________________________________________________________________________Installation
3 - 1
Figure 3-1

3. Sample thermocouple input: Provides sample thermo-
couple signal to controller.
4. Recorder output: Provides chamber and/or sample tem-
perature information to recorder for hard copy graphs.
5. RS232 Port: Computers/printers interface
6. Chamber output: Provides signal from controller to con-
trol fan, cool and cool + solenoid valves, and heater.
7. Power to freezing chamber: Provides power for fan, cool
and cool+, solenoid valves and heater.
8. Power to recorder: Connect to a power supply.
Note: Before beginning a program run, turn the recorder power
“ON”, the chart drive power “ON” and lower the pen to the
chart paper, using the pen lever arm. For details on recorder
operation, refer to the supplemental recorder manual.
3.4 Connecting the Liquid Nitrogen Transfer Hose
Warning! Some liquid nitrogen supply tanks con-
tain a pressure capable of supplying liquid, or
gaseous nitrogen at up to 235 PSIG. Under such
conditions, change the supply tank to one with a
22 PSIG setting to provide compatibility.
Using a 7/8” open end or an adjustable wrench, connect
the transfer hose:
1. Connect hose to input flare fitting on back of chamber.
2. Connect other end of hose to the LIQUID side of the
LN2tank.
Important! The supply tank has a liquid fitting and a vent
fitting. Be sure to connect the nitrogen transfer hose to the
liquid fitting.
Models 1010 and 1011________________________________________________________________________Installation
3 - 2
POWER SWITCH
CHAMBEROUTPUT RS232PORT REC.OUTPUT SAMP CHAM
Figure 3-2

1. Control alarm, fan and cooling mode operation.
2. Used to select four specific operating modes of the con-
troller: Program, Run, Warm or Idle.
Note: Unit is in Idle Mode if no displays are illuminated.
3. Six LED modules display the system status of the unit
during a program cycle.
4. Three keys control thermocouple scanner operation for
sample only, chamber only, or sample and chamber
alternately and relay temperature information to strip
chart recorder.
5. Digital LED display of selected program, section num-
ber, hold time, elapse time and setpoint temperatures.
6. Four indicators allow user to toggle display of pro-
grammed/actual chamber or sample temperature.
7. Up and down arrow keys allow user to advance or
retreat through the programs to subsequent or previous
sections.
8. Used to select and enter numerical values and functions.

Section 4 - Operation
4.1 Control Panel Operation
The controller face plate is composed of a sealed mem-
brane keyboard, with small indicating LED’s and digital dis-
plays. The keyboard is arranged in sections, separated into
groups of outlined keys by function.
a. Control Section
The function of this set of keys on the control
panel is the alarm, fan and cooling mode operation.
CONTROL NAME FUNCTION
PWR (Indicator) Indicator illuminates when the unit is
turned “on”.
Note: The power switch is located on the rear of the unit,
directly above the power cord.
ALRM Controls power to the audible alarm
circuit, when controller is in IDLE
mode. Red LED indicates alarm is
enabled. Will silence audible alarm.
Note: Alarm sounds at cycle end (freezing run and warm-
ing run) and when error conditions occur.
FAN * Controls power to the fan circuit in
the freezing chamber, when con
troller is in IDLE mode. Red LED
illuminates only when fan is actually
energized.
*It is not necessary to turn the fan “on” before each run. It
will automatically function when the unit is in Run Mode.
COOL Controls the liquid nitrogen solenoid
valve circuit, in both automatic and
manual modes, as follows:
Automatic Operation: Enables COOL circuit when con-
troller is in IDLE mode. This will always be enabled.
The red LED illuminates to indicate that the COOL circuit
is enabled.
Manual Operation: Opens the Cool liquid nitrogen solenoid
valve, when depressed, and closes it when released; under
the following conditions:
• The COOL circuit has been enabled (while running in the
“unlimited access mode”).
• The controller is running a program, or is in the “IDLE”
mode.
Note: After the COOL keypad is released, automatic con-
troller operation will resume.
CONTROL NAME FUNCTION
COOL+ Controls the liquid nitrogen solenoid
valve circuit, for the injection of
additional LN2 (for accelerated
freezing rates) as follows:
Automatic Operation: Enables COOL + circuit when con-
troller is in IDLE mode. This will be enabled only if it has
been programmed into the section being viewed.
The red LED illuminates to indicate that the COOL + cir-
cuit is enabled. This must be programmed into the memory
in order to be enabled.
Important: The COOL+ circuit must be programmed in,
for the computer to energize the COOL+ liquid nitrogen
solenoid valve.
Manual Operation: Opens the COOL+ liquid nitrogen sole-
noid valve, when depressed, and closes it when released;
under the following conditions:
• When the COOL+ circuit has been enabled (while running
in the “unlimited access mode”).
• When the controller is running a program, or is in the
“IDLE” mode.
Note: After the COOL+ keypad is released, automatic
controller operation will resume.
b. Mode Select
This set of keys is used to select specific
operating modes of the controller: Program,
Run, Waarm, Full Access run.
Note: Unit is in Idle Mode if no displays are illuminated.
CONTROL NAME FUNCTION
PROG Selects the programming mode. Use
when operator wishes to change any
internal program memory.
RUN Press the RUN key to start a pro-
gram; Press also to resume the pro-
gram, during a WAIT program
section.
Note: Program will not proceed during a WAIT section,
until the RUN key is pressed.
WARM Starts the automatic WARM cycle.
Causes freezing chamber to warm to
+25°C.
Note: The PROGRAM NUMBER and SECTION NUM-
BER will display 0.0. When the freezing chamber reaches
+25°C, the controller will hold the temperature at +25°C. It
will cycle LN2 if it is supplied.
Models 1010 and 1011__________________________________________________________________________Operation
4 - 1
Figure 4-1
Figure 4-2

CONTROL NAME FUNCTION
Note: Pressing a keypad whose LED’s is already illuminat-
ed, will cause the computer to exit from that mode with the
exception of the “RUN” mode.
(IDLE Mode) This action will return the controller
to the IDLE mode (no displays in
Mode Select section are illuminated).
The term IDLE MODE will be used
during installation and troubleshoot-
ing of the unit.
c. System Status
Six LED modules display the system
status of the unit during a program cycle.
INDICATOR NAME FUNCTION
COOL Indicator illuminates to indicate
when the COOL solenoid valve is
open, injecting liquid nitrogen into
the freezing chamber.
COOL + Indicator illuminates to indicate
when the COOL + solenoid valve is
open, injecting additional liquid
nitrogen into the freezing chamber.
HEAT Illuminates when heating circuit is
energized.
WAIT Illuminates when the program is
being run, indexing through, or being
edited contains a WAIT section.
ERROR Illuminates when the controller
senses the presence of an error
condition.
END Illuminates when the program is
being run, indexing through, or being
edited contains a END section.
d. TC Scanner
Three keys control thermocouple
scanner operation for sample only, cham-
ber only, or sample and chamber alter-
nately. This temperature information is
then relayed to the strip chart recorder.
CONTROL NAME FUNCTION
SAMP Selects the Sample thermocouple as
the temperature to be sent to the
recorder. The red LED illuminates
when the controller is sending out
Sample temperature to recorder.
CHAM Selects the Chamber thermocouple
as the temperature to be sent out to
the recorder. The red LED illumi-
nates when the controller sending out
chamber temperature to the recorder.
SCAN Tells computer to scan both thermo-
couples at fixed rate (two seconds
for the chamber thermocouple,
followed by ten seconds for the
sample thermocouple) and outputs
the information to the recorder.
Note: The SAMP and CHAM LEDs will alternately illumi-
nate during scanning to indicate the thermocouple being
scanned at that moment.
Note: The SCAN keypad does not have a LED indicator.
e. Program Display
The digital LED display of
the selected program, section
number, rate, hold time, elapse
time, and setpoint temperatures.
f. Display
Four indicators permit the operator to toggle the
display of the programmed/actual chamber or cham-
ber/sample temperature.
CONTROL NAME FUNCTION
PROG/ACT Manually toggles the display section
between the programmed, or actual
temperature value.
Note: When in PROG mode, automatically toggles
between programmed temperature and target temperature
for that programmed section.
CHAM/SAMP Must be in ACT, manually switches
the display section between the CHAM and SAMP value.
Models 1010 and 1011__________________________________________________________________________Operation
4 - 2
Figure 4-3
Figure 4-4
Figure 4-5
Figure 4-6

g. Prog. Adv.
The up and down arrow keys allow the user to
scroll through the programs.
CONTROL NAME FUNCTION
UP ARROW Increments through the program to
the next section; repeats approxi-
mately three times per second if held
depressed.
DOWN ARROW Decrements through the program to
the previous section; repeats approxi-
mately three times per second if held
depressed.
Note: In full access mode, these arrow keys raise or lower
the setpoint.
h. Numerical and Special Functions
This keypad is used to select
and enter numerical values and
functions when programming the
controller.
CONTROL NAME FUNCTION
NUMERIC KEYS Represent numerical values, zero
through nine.
HOLD TIME Used when programming to enter a
specific amount of time (in minutes)
that the temperature, within a cycle,
is to be held.
COOL + Used when programming to select a
COOL+ function, for rapid cooling.
Important: Should be used on all low temperature pro-
grams.
SAMP Used when programming to enter a
setpoint value, corresponding to the
SAMPLE temperature. When used
with “HOLD”, permits seconds to be
programmed.
ENT Used to enter and store values and
parameters, previously keyed into
the computer memory (active only
when in Program mode).
END Used to program a “label” the end or
last section of a program (active only
when in the PROG position).
CLR -/. Used when programming to remove
and entry (active only when in the
PROG position).
Used when programming a negative
number.
Used to enter a decimal point when
entering a program number in the
user-definable half of memory.
4.3 System Operation
The Model 8018 Programmable Controller regulates the
flow of liquid nitrogen to the freezing chamber. Additionally, it
supplies temperature information to the strip chart recorder.
The freezing cycle consists of the following steps and oper-
ations:
Note: Typically the biological sample is mixed with a solution
of cryoprotective agent and placed in a plastic cryoampule.
1. STARTING TEMPERATURE. Sample temperature
prior to being loaded into the freezing chamber. The
sample is usually at room temperature (+22C) or ice
bath temperature (+4C).
2. LIQUID PHASE COOLING. The rate at which the liq-
uid sample is cooled. A typical rate of cooling is
1°C/min.
Note: A regulated flow of LN2 into the freezing chamber is cir-
culated by an internal electric fan. This assures even sample
cooling.
3. SUPER COOLING. The temperature drop achieved just
prior to liquid-to-solid phase change. It is generally three
to four degrees centigrade below the freezing point.
4. PHASE CHANGE. The beginning of the liquid-to-solid
phase change process, characterized by a rapid increase
in temperature, from super cooled temperature to freez-
ing temperature. To minimize temperature rise during
phase change, chamber temperature is rapidly dropped.
This rapid cooling absorbs latent heat of fusion, mini-
mizing any temperature rise above the freezing point.
Note: Refer to the chart (Figure 4-1) at the end of this section
for the freezing point temperature of the biological sample cor-
responding to the percentage of cryoprotective agent added to
the sample.
5. SOLID PHASE I FREEZING. The freezing rate
selected for solid phase cooling is independent of liquid
phase cooling rate. This rate is determined by the opera-
tor, as required, and is typically the same as liquid phase
cooling rate of 1°C/min.
Models 1010 and 1011__________________________________________________________________________Operation
4 - 3
Figure 4-7
Figure 4-8

6. END SOLID PHASE I FREEZING. The final temper-
ature achieved in Solid Phase I freezing cycle. It is typi-
cally set between -30°C and -50°C, as required by the
operator.
7. SOLID PHASE II FREEZING. The freezing rate is
independent of the Solid Phase I freezing rate. At -30°C
to -50°C, the sample is considered safe. This permits the
temperature to be dropped at a much faster rate, such as
10°C to 20°C per minute.
Note: Researchers generally find that a Liquid Phase cooling
rate of 1°C/min, a solid Phase I freezing rate of 1°C/min, and a
Solid Phase II rate of 10°C/min will provide maximum recov-
ery of viable cells, while minimizing freezing cycle time.
8. END FREEZE TEMPERATURE. The final temperature
achieved at Solid Phase II freezing cycle. It is typically
set at -90°C, and maintains freezing chamber tempera-
ture until sample is ready to be transferred to long term
storage.
The following chart shows the relationship between the
freezing point temperature of biological samples and the per-
centage of cryoprotective agent added.
Chart 4-1
% Cryoprotective Agent Freezing Point Temperature
(DMSO, Glycerol, Etc.)
0 0°C
5% -2°C
7.5% -3°C
10% -4°C
12.5% -5°C
15% -6°C
20% -8°C
30% -12°C
4.4 Initiating a Freezing Run
After the freezing system has been connected as illustrated
in Figure 3-1, proceed as follows:
1. Turn on the controller.
Note: Upon start-up, the controller will run through a complete
lamp test, and return to the “Idle Mode” (no lights are illumi-
nated in the “Mode Select” section of the front panel).
2. Press the power switch on the strip chart recorder, and
permit the system to warm-up for 20 minutes.
3. Uncap and lower the pen to the graph paper.
4. Calibrate the recorder as follows:
a. Press “CHAM” in the TC Scanner section of the
controller. The recorder pen should move to zero on
the strip chart recorder. If it does not, dial it to zero,
using the black knob on the recorder.
b. Press “SAMP” in the TC Scanner section of the
controller. The recorder pen should move to -180°C.
If it is out of calibration, adjust using the set screw
adjacent to the zero knob until the pen reaches
-180°C.
c. Recheck zero calibration as directed in step 4A.
5. To select program:
User Program - Press the program number, the decimal
point, (and section number, if required).
Factory-set Program - Press the program number (and
section number, if required).
Note: Program and Section of desired program may be select-
ed by using the up/down arrow keys to locate the program
number and section number that you wish to run. (Up arrow
means increase in numbers; down arrow means decrease in
numbers.)
6. Depress “RUN” to start.
4.5 Factory Pre-set Programs
PROGRAM #1
Standard 1.2/2.0ml w/7% to 12% Cryoprotectant Agent
Section 1 Wait @ 4° Cool+
Section 2 1 degree/minute to -4° Sample
Section 3 25 degrees/minute to -40° Cool+
Section 4 15 degrees/minute to -12° Cool+
Section 5 1 degree/minute to -40° Cool+
Section 6 10 degrees/minute to -90° Cool+
Section 7 End
PROGRAM #2 Standard Microtest Tray Freeze
Section 1 Wait @ 4° Cool+
Section 2 1 degree/minute to -40° Sample
Section 3 10 degrees/minute to -90° Cool+
Section 4 End
PROGRAM #3 Standard Embryo Freeze (0.25 to 0.50ml
straw)
Section 1 Wait @ +20° Cool+
Section 2 1 degree/minute to -4°
Section 3 0.5 degree/minute to -7°
Section 4 5 minute hold time at -7°
Section 5 0.3 degrees/minute to -35°
Section 6 0.1 degrees/minute to -37°
Section 7 End
Models 1010 and 1011__________________________________________________________________________Operation
4 - 4

PROGRAM #4 Standard Blood Bag/Bone Marrow
75-150ml, w/7% to 10% Cryoprotectant Agent
Section 1 Wait @ 20° Cool+
Section 2 1 degree/minute to -6° Sample
Section 3 25 degrees/minute to -50° Cool+
Section 4 15 degrees/minute to -14° Cool+
Section 5 1 degree/minute to -45° Cool+
Section 6 10 degrees/minute to -90° Cool+
Section 7 End
PROGRAM #5 Skin Packet 4” x 6” w/10%
Cryoprotectant Agent
Section 1 Wait @ +4° Cool+
Section 2 1 degree/minute to -5° Sample
Section 3 20 degrees/minute to -40° Cool+
Section 4 10 degrees/minute to -10° Cool+
Section 5 1 degree/minute to -35° Cool+
Section 6 10 degrees/minute to -90° Cool+
Section 7 End
PROGRAM #6 2ml Sample w/7%-12% Cryoprotectant
Agent
Section 1 Wait @ +4° Cool+
Section 2 1 degree/minute to -4° Sample
Section 3 20 degrees/minute to -45° Cool+
Section 4 15 degrees/minute to -10° Cool+
Section 5 0.5 degree/minute to -20° Cool+
Section 6 1 degree/minute to -80° Sample
Section 7 End
4.6 Programming the Controller
The Model 8018 Programmable Controller permits the
operator to create freezing programs suited to a specific need.
The operator may enter, by touching the appropriate keypads, a
sequence of operating command, and parameters into the com-
puter memory. The memory is retained even when a power loss
occurs, and is available upon power-up at the touch of a few
keys.
A “lock-out” feature, requiring an access code protects the
programs from unauthorized tampering, or accidental change.
Note: The user pro-
grammable section of
memory is identified
as “Program Number”
(1-9), decimal point,
followed by “Section
Number” (1-9), as
shown at the right.
Nine programs, each consisting of nine sections, are user
programmable. Each memory section is designed to hold infor-
mation necessary to perform a single task. Each freezing task
can consist of only one of the following:
1. A “HOLD” time, while maintaining a chamber tempera-
ture setpoint.
2. A “WAIT” function, while maintaining a chamber tem-
perature setpoint.
3. A “RATE C/MIN” temperature increase, or decrease,
until a “SAMPLE” or “CHAMBER” temperature reach-
es setpoint.
4. END
By programming one or more sections in sequence, the
user can create programs. The total effect of all tasks, running
sequentially make for a flexible freezing program.
This section will provide user with step-by-step instruc-
tions on entering user defined programs into the Model 8018.
The following is a sample freezing program for a 2ml sam-
ple with a predetermined freezing point of -4°.
Example: User Defined Program
Program #1
Section #1: 4 Degrees start temperature
Section #2: 1 Degree per minute to -4 degrees sample
Section #3: 30 Degrees per minute to -60 degrees
Section #4: 15 Degrees per minute to -12 degrees
Section #5: 1 Degree per minute to -45 degrees
Section #6: 10 Degrees per minute to -90 degrees
Section #7: END
Programming Notes:
1. Cool + should be programmed into every temperature
section. This is done by depressing “COOL +” on the
keypad in the second step of each section.
2. When programming a sample switch point, it is neces-
sary to depress “SAMP” key. It will automatically be a
chamber setpoint unless the “SAMP” key is depressed.
Note: Refer to the chart on the following pages, “Entering the
Example User defined Program”.
Models 1010 and 1011__________________________________________________________________________Operation
4 - 5
Figure 4-9

Entering the Example User Defined Program:
PROGRAMMING ACTION KEYSTROKE SEQUENCE EXPLANATION
Beginning-Selection of Program Press “PROG”, 1, 2, 3, “ENT” This is the factory programmed access
Number and Section Number Press Desired Program Number, code.
Section Number. The controller will automatically supply the
Example: Program 1, Section 1, “ENT” decimal point, to indicate the “user pro-
grammable” sector of memory is being
accessed.
Programming Section #1 Press 0, ENT When “0” is entered, the display area
Start Temp goes blank and the word “WAIT”* appears.
Note: This indicates that you are instruct-
ing the controller to go to the “Start Temp”
and maintain that temp until the run key is
pressed again.
Press 4, COOL+, ENT This indicates that the start temperature
selected is +4°C.
*Note: When the program is running and comes to a programmed WAIT command, the program will not advance until the operator
presses the RUN key.
Programming Section #2 Press 1, 0, ENT This enters a rate of 1 degree per minute.
Liquid Phase Rate Note: The “0” must be pressed to move the
decimal point to the proper position.
Press -, 4, SAMP, COOL+, ENT This is the “start phase change” which is
usually a predetermined sample freezing
point.
Note: This is the only temperature setting
which is recommended to be a “sample
switch point”.
Programming Section #3 Press 3, 0, 0, ENT This is selects a rate of 30° per minute.
Temp Drop Press -, 6, 0 COOL+, ENT This selects a temperature drop of -60°C.
Programming Section #4 Press 1, 5, 0, ENT This selects a rate of 15 degrees per minute.
End of Phase Change Press -, 1, 2, COOL+, ENT This is the end of Phase Change tempera-
ture, which is generally 8 to 10 degrees
colder than the start of phase change.
Programming Section #5 Press 1, 0, ENT This enters a rate of 1 degree per minute.
Solid Phase Freezing Rate Press -, 4, 5, COOL+, ENT Note: The “0” must be pressed to move the
decimal point to the proper position.
This programs the controller to run this
rate until the chamber reaches -45°C.
Note: The sample is generally considered
safe when it reaches between -30C and
-50°C.
Programming Section #6 Press 1, 0, 0, ENT This is selects a rate of 10° per minute.
Solid Phase Freezing Rate II Note: The “0” must be pressed to move the
decimal point to the proper position.
Press -, 9, 0, COOL+, ENT This selects a Target temperature of -90°C.
Programming Section #7 Press END, COOL+, ENT Note: Use the down arrow key, to scroll
End back through the program to check it’s
accuracy; and to return to Section 1.1.**
**Note: You must return to Section 1.1 of the program before pressing “RUN” to execute the program.
Models 1010 and 1011__________________________________________________________________________Operation
4 - 6

4.7 Running the Program in the Unlimited Access
(Manual) Mode
IMPORTANT: Manual operation of the controller, as a gener-
al rule, should be avoided. After manual operation is discontin-
ued, program control will resume and compensate for any man-
ual adjustment to the program.
Under normal running conditions, the controller is in a
“locked” or “limited” access mode. When running in this mode,
no manual operation of the controller can take place. If the pro-
gram run needs to be modified, the controller must be returned
to the “unlimited access” mode.
Depress PROG, 1, 2, 3, ENT
The RUN light will blink on and off, while it continues to
run. The blinking indicator indicates that the controller is now
in the “unlimited access” mode.
Note: While in this mode, program segments may be modified
(for this program run only), such as coolant or heat may be
added by pressing the appropriate keys.
1. Add coolant by depressing COOL AND COOL+ on the
left side of the unit under Control.
2. Add COOL+ to a Program section (if it was not entered
at programming) by depressing COOL+ on the numeri-
cal display on the right side of the controller.
3. Add heat by using the up arrow for an increase in tem-
perature; or coolant by using the down arrow for a
decrease in temperature.
4.8 Aborting a Program Run
If it becomes necessary to abort the program run, return the
controller to the “unlimited access” mode as follows:
1. Depress PROG, 1, 2, 3, ENT.
2. Depress the RUN key while the controller is in the
“unlimited access” mode.
Note: If the program is in a “Wait” section, it will be necessary
to depress RUN twice, as the first depression of the key
advances the program only to the next section.
4.9 Programming the Access Code
The access code “1, 2, 3” was factory-set prior to ship-
ment of the Model 8018. If the user/operator desires to change
the access code, perform the following steps.
Example: Change access code to 4, 5, 6.
Depress keys in this sequence: PROG, 1,2,3, ENT, CLR,
4,5,6, ENT.
Note: If the new three-digit access code is not entered within
ten seconds, the controller will automatically revert back to the
previous access code.
4.10 Programming the Error Limit Setpoints
The error limit setpoints are designed to alert the operator
to a temperature deviation in either the CHAMBER or the
SAMPLE, by sounding an audible alarm.
Alarm conditions could result from one or more of the
following:
•Loss of cooling source (liquid nitrogen)
•Lack of LN2 pressure
•Freezing chamber (solenoid valve stuck open or closed)
•Fan motor overload tripped
•Exhaust blockage
•Incorrect Programming
The error limit setpoint has been factory programmed for
1 °, 5 minutes, and under most circumstances should not be
changed by the operator. If the temperature deviates 1° from
the programmed temperature (for 5 minutes duration) the
audible alarm will sound. The error limit setpoint was pro-
grammed as follows:
•Example: 1° for 5 minutes
•Keystrokes: PROG, 1, 2, 3, ENT, 0, ENT, 1, ENT, 5,
ENT, PROG, 0.
Note: Only one set of error limit setpoints can be stored in
memory at one time. They are applicable to all the programs.
4.11 Programming a Sample Freezing Point
Determination Run
The Start Phase Change Temperature is the temperature at
which the SAMPLE freezes. To determine the freezing point of
a new biological sample, refer to the chart at the end of this
manual, or execute the sample freezing run on the next page.
Models 1010 and 1011__________________________________________________________________________Operation
4 - 7

Sample Freezing Point Determination Run
PROGRAMMING ACTION KEYSTROKE SEQUENCE EXPLANATION
Beginning-Selection of Program Press “PROG”, 1, 2, 3, “ENT” This is the factory programmed access code.
Number and Section Number Press Desired Program Number, The controller will automatically supply the decimal
Section Number. point, to indicate the “user programmable” sector of
Example: Program 1, Section 1, memory is being accessed.
“ENT”
Programming Section #1 Press 0, ENT When “0” is entered the display area goes blank and
Start Temp the word “Wait”* appears.
Note: This indicates that you are instructing the
controller to go to the “Start Temp” and maintain
that temp until the run key is pressed again.
Press 4, COOL+, ENT This indicates that the start temperature selected is
+4°C.
Programming Section #2 Press 1, 0, ENT This enters a rate of 1° per minute.
Liquid Phase Rate Note: The “0” must be pressed to move the decimal
point to the proper position.
Press -, 5, 0, COOL+, ENT This selects a target temperature of -50°C
END, ENT
*Note: When the program is running and comes to a programmed WAIT command, the program will not advance until the operator
presses the RUN key.
Models 1010 and 1011__________________________________________________________________________Operation
4 - 8
After the run has been completed, review and analyze the
strip chart cooling trace, it should be similar to the chart titled
“DETERMINING THE START PHASE CHANGE TEMPER-
ATURE”, located at the rear of the manual.
The trace will show a sudden temperature rise, when the
phase change portion of the cycle has been reached. The linear
temperature trace, just after the sudden temperature rise (release
of latent heat) is the freezing point or start of Phase Change
Temperature (Heat of Fusion Plateau Temperature).
Make the following connections:
Note: Refer to the the serial tag on the rear of the controller
for specific electrical requirements.
1. POWER TO THE CONTROLLER: With the controller
power switch set to the “OFF” position, connect the con-
troller power cord to a grounded power source.
2. Chamber thermocouple input: Provides chamber thermo-
couple signal to controller, from freezing chamber.
4.12 RS-232
The software within the Model 8018 controller has
special features for remote control by another computer, such
as an IBM (or an IBM compatible) PC. The remote computer
talks to the Model 8018 through the serial RS-232 communica-
tion port on the rear of the controller assembly.
Two modes of serial communication are provided. The first
mode is designed to operate as a background task, when the
controller is in the “RUN” or “WARM” mode. It will format
the “RUN” mode parameters into a simple display, and spool it
out the serial port. This allows the user to attach a CRT termi-
nal to the serial port, and to monitor the “RUN/WARM” mode
status. The information provided by the spooler mode is the
same as that displayed on the front panel of the micro con-
troller.
The second serial communication mode is an executive
monitor. It disables control of the micro controller through the
front panel, and turns over complete control of the system to a
master controller attached to the serial port. It could be used in
applications where the operator wants the micro controller to be
completely controlled by a remote computer. Any type of com-
puter can be used for this mode, provided that it is able to com-
municate with the Model 8018 microcontroller, through a serial
RS-232 link, which can be configured to the following parame-
ters:
9600 BAUD
8 Data bits
1 stop bit
Parity test disabled
Space parity transmitted

The first communication mode is a data spooler, and it is
used when the micro controller is operating in either the
“RUN” or “WARM” mode. The micro controller is still operat-
ed through the front panel, and operation of the data spooler is
transparent to all system activity. The spooler mode is turned on
by pressing the “>” key and it is turned off by pressing the “<”
key. When the spooler mode is turned on, the following display
is repeatedly sent to the device attached to the serial port:
PS=0101
FN=11
P1=0000
P2=0028
CT=+004.0
CH=+026.3
SP=+026.8
ST=801C
At the end of each display, the following “Cursor-to-
Home” character sequence is issued: ESC, “=”, “ “, “ “.
The first line of the display shows the program and section
that is currently being executed. The second line shows the
function that was programmed for that section. This is a coded
number that contains the information about the programming
function, and whether the COOL + output is to be used for
cooling. When the tens digit of the number is 0, the use of
COOL+ is disabled. When the tens digit is 1, the use of
COOL+ is enabled. The following table lists the functions
defined by the units digit:
0 = WAIT at the last specified control temperature
1 = WAIT at the control temperature specified by parameter P2
2 = WARM mode function code
3 = RAMP at the rate specified by parameter P1 until the cham-
ber reaches the temperature specified by parameter P2
B = RAMP at the rate specified by parameter P1 until the sam-
ple reaches the temperature specified by parameter P2
4 = HOLD for the number of minutes specified by parameter
P1, at the last specified control temperature
5 = HOLD for the number of minutes specified by parameter
P1, at the control temperature specified by parameter P2
6 = END function code
7 = GOTO function code, program and section are in parameter
P1
The third and fourth lines of the display show the auxiliary
parameters that were programmed into the current section. They
are displayed in a hexadecimal format (the base sixteen number
system). The next three lines show the current Control temper-
ature, Chamber temperature, and Sample temperature in
degrees Centigrade. The last line shows the current system sta-
tus. This is also a hexadecimal number, with each bit represent-
ing a different condition. The following table lists the meaning
of each bit of the status word. Bit15 is in the left-most position,
and bit0 is in the right-most position. All of the unspecified bits
are always RESET.
bit15 = WARM mode alarm hold-off is disabled when SET
bit10 = error has been marked when SET
bit9 = alarm enabled (LED is on above ALARM key) when
SET
bit8 = alarm is on (beeper is sounding) when SET
bit7 = sample TC is selected for A/D conversion when SET
chamber TC is selected for A/D conversion when
RESET
bit4 = FAN is on when SET
bit3 = COOL solenoid is on when set
bit2 = COOL+ solenoid is on when set
bit1 = HEAT relay in on when SET
bit0 = BEEPER is on when SET
The second communications mode is the system monitor. It
allows the user to take over complete control of the micro con-
troller through the serial port, and it is entered by pressing the
“#” key. When the monitor mode is entered, all temperature
control stops, all temperature control outputs are turned off, the
front panel display is blanked, and the characters “ACS” are
shown on the center display to indicate that the micro controller
is being accessed through the serial port.
Upon entry to the monitor mode, the following clear screen
sequence is issued: ESC, “:”. This is a valid clear screen
sequence for a Lear-Siegler ADM 3A CRT terminal. After the
clear screen sequence is sent, a menu of commands is displayed
and the monitor prompt is issued as follows:
GENERATE CHECK SUM-----A bbbb eeee
EXAMINE MEMORY-----------E bbbb eeee
MODUIFY MEMORY-----------M aaaa
RAM TEST-------------------------R bbbb eeee
LOAD USER PROGRAMS------L
DUMP USER PROGRAMS------D
RESUME EXECUTION----------X
ON-LINE:
Models 1010 and 1011__________________________________________________________________________Operation
4 - 9

At this point, the user can completely interrogate the
Model 8018 micro controller by issuing various commands. A
command is invoked by typing its associated letter and provid-
ing any secondary parameter, where required. The secondary
parameters are always hexadecimal numbers, and they should
be delimited by one space. Pressing the CONTROL-X character
at any point in the monitor mode will cause the prompt to be
issued immediately. Press this key to “Cancel” a command
before the RETURN key is pressed, or “Abort” a function once
it has been invoked.
The “A” command generates the proper validation code for
a specified portion of the memory map. It is a special function
that is provided for applications where the user (or remote com-
puter) desires to change the USER PROGRAM AREA of the
micro controller. Since the check-sum for the data area must
Models 1010 and 1011__________________________________________________________________________Operation
4 - 10
ON-LINE: E D000 D19F
D000 02 2B 06 00 00 00 00 06 00 00 00 00 06 00 00 00 .+..............
D010 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 ................
D020 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 ................
D030 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 ................
D040 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 ................
D050 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 ................
D060 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 ................
D070 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 ................
D080 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 ................
D090 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 ................
D0A0 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 ................
D0B0 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 ................
D0C0 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 ................
D0D0 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 ................
D0E0 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 ................
D0F0 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 ................
D100 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 ................
D110 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 ................
D120 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 ................
D130 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 ................
D140 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 ................
D150 00 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 ................
D160 06 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 ................
D170 00 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 ................
D180 00 00 00 06 00 00 00 00 06 00 00 00 00 06 00 00 ................
D190 00 00 06 00 00 00 00 02 00 01 00 32 01 02 03 0A ...........2....
always be correct, in order to successfully supply a new tem-
perature control program, the sections must be entered directly
(using the “L” or “M” command. The last step of the alteration
procedure is to place the new check-sum into memory by using
the “M” command.
The “E” command provides a formatted display of the por-
tion of the memory map specified by the two input parameters.
The display shows sixteen values per line in the following for-
mat: address, data in hexadecimal format delimited by spaces,
followed by the same data interpreted as ASCII characters. For
the second display field, any value that can not be displayed as
a printable ASCII character is shown with a “.” character. The
following example shows how the display would appear if the
user supplied the beginning and ending address of the USER
PROGRAM AREA, after it has been reset to END functions.