Thermo Scientific 1300 Series User manual

1300 Series A2
Class II, Type A2 Biological Safety Cabinet
Operating Manual 7011355 Rev. 10

Thermo ScientificThermo Scientific
MANUAL NUMBER 7011355
10 25132, 25040 12/9/08 Updated text of stand assembly, window opening positions ccs
9 25000/HD-1528 9/29/08 Added distance required between counterweight and other objects (3 in) ccs
8 24759/HD-1554 9/10/08 Added China models with line cord and receptacle ccs
7 24886/HD-1528 9/5/08 Added Certification Testing from service manual ccs
6 24783/HD-1528 7/15/08 Added warranty ccs
5 24741/HD-1550 6/17/08 Added universal piping access points (pg 2-7) ccs
4 24680 6/3/08 Corrected model numbers in Specifications ccs
3 24386/HD-1527 5/29/08 Updated valve artwork from Broen to Water Saver (Page 2-6) ccs
2 24608/HD-1548 5/1/08 Clarified artwork and instructions for unit/stand assembly ccs
1 24300/HD-1527 11/7/07 Updated electrical ratings, changed format, added transition and valve info ccs
0 -- 9/10/07 Original (50111618) ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
1300 Series A2 i
This manual covers the models shown below:
Dear User,
Congratulations on your purchase of a Thermo Scientific 1300 Series Class II,
Type A2 bio-safety cabinet. Your 1300 Series A2 bio-safety cabinet has been
tested and certified in accordance to NSF/ANSI 49, and is designed to protect
the user, the environment, and your research from harmful substances and cross-
contamination. This user’s manual provides instructions on how to use the 1300
Series A2 most effectively and safely.
The 1300 Series offers a unique range of product features that will enhance your
safety and improve overall operating efficiency. Should you have any questions on
using this product or need further explanation of any of it’s features, please
contact Thermo Scientific Technical Services at the phone numbers listed on Page
iv, or your local distributor.
1354 4 ft 230V, China
1355 4 ft 120V
1356 4 ft 230V
1357 6 ft 120V
1358 6 ft 230V
1359 6 ft 230V, China
1384* 4 ft 230V, China
1385* 4 ft 120V
1386* 4 ft 230V
1387* 6 ft 120V
1388* 6 ft 230V
1389* 6 ft 230V China
* Includes cabinet, 30 inch fixed-height stand, UV light and armrest.

Thermo Scientificii 1300 Series A2
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Scientific makes no representations or warranties with respect to this manual. In
no event shall Thermo Scientific be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
©2007 Thermo Scientific. All rights reserved.

Thermo Scientific 1300 Series A2 iiiThermo Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Scientific
has contracted with one or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them. Further information on
Thermo Scientific’s compliance with this directive, the recyclers in your country and information on
Thermo Scientific products will be available at www.thermo.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv 1300 Series A2 Thermo Scientific
Preface
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-740-373-4189 FAX
http://www.thermo.com Internet Worldwide Web Home Page
Service E-Mail Address
Thermo Scientific
Controlled Environment Equipment
401 Millcreek Road, Box 649
Marietta, OH 45750
Our staff can provide information on pricing and give you quotations. We can
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our staff can supply technical information about proper setup, operation or
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
International customers, please contact your local Thermo Scientific distributor.
Sales Support
Service Support
[e-mail]

1300 Series A2 vThermo Scientific
Table of Contents
Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Safety Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
HEPA Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Paper Catch Grids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Use of the Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Unit Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Chamber Lighting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
UV Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Work Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Optional Exhaust Transition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Exhaust System Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Moving the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-4
Service Valve Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-5
Universal Piping Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-7
Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Unit w/wo Stand . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Unlock Counterweight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-4
Installation Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-5
Locating a Certifier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-6
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Calibration Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Unit Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Operating Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Loading the Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Working Recommendations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-6
Procedure Interrupt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-7
Unit Shut-down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-8
Unit Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-8
Section 1
Section 2
Section 3
Section 4

vi 1300 Series A2 Thermo Scientific
Cleaning / Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Wipe/Spray Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Pre-disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Final Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
UV Disinfection after Wipe/Spray Disinfection . . . . . . . . . . . . . . .5-3
Disinfection with Formaldehyde . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean Exterior Surfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean the Drain Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Clean the Paper Catch Grid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Sample Chamber Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Optional UV Lights . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Replacements and Repairs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Unit Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Certification Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Data Log/Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Table of Contents
Section 5
Section 6
Section 8
Section 7
Section 9

1300 Series A2 1-1Thermo Scientific
Section 1 Description
The plenum assembly consists of a plenum for the downflow blower and a
plenum for the exhaust air blower. Each blower includes a filter. The
exhaust air is discharged through an opening in the top of the cabinet.
Chamber lighting includes one fluorescent bulb for 4 foot models and two
for 6 foot models.
The optional, factory-installed UV light is installed in the ceiling at the
front of the sample chamber.
Two service valve access ports are located on each side panel. When
installing service valves, remove the center of the port plugs at the
perforations.
Figure 1-1. Unit Components
Plenum
Power cord
Electrical enclos
u
Light dome
Control panel
Handle
Front window
Service valve
access ports
Stand (optional)
Service
valve
Exhaust
test hose
Work
tray
Armrest
Supply
test hose
Accessory
outlets
Plenum
access panel
o
wnflow blower
Exhaust blower
RS-232
Connection
Fuseholders (2)
Exhaust opening

1-2 1300 Series A2 Thermo Scientific
Section 1
Description
Safety Systems
The optional stand may be ordered in three configurations; a fixed 30”
working height, a manually adjustable 30” to 38” working height, and a
fixed 34” working height with attached casters.
A stainless steel one-piece work surface is standard. Optional armrests
ensure a comfortable working position for the user and minimize
blockage of airflow at the front grille.
Internal outlets, provided as the power source for accessories, are provided
standard in the following configuration:
1354, 1355, 1359, 1385: one duplex right side
1357, 1387: two duplex left and right side
1356, 1384, 1386: one single right side
1358, 1388, 1389: two single right and left side
Test hoses (aerosol challenge ports) are located under the work tray. The
supply plenum hose is located on the left and the exhaust plenum hose on
the right.
Note Do not remove the caps of the two test hoses to check for supply
and exhaust airflow. s
Negative pressure air system
A negative pressure air system combines with HEPA filters in the supply
and exhaust airflow for personal and product protection.
Personnel protection
Inflow air along the entire working opening at a constant high velocity
prevents leakage from the work opening of the chamber.
Exterior air pressure being higher than the internal air pressure creates
negative pressure, ensuring containment in case of cabinet leakage.
Product protection
Steady airflow within the air system ensures constant downflow, allow-
ing the HEPA filters to remove contaminants so that the samples are
always surrounded by ultra-pure air. Harmful particles are not carried
over the sample chamber (protection from cross-contamination).
HEPA filters
The downflow air within the chamber and the exhaust air are cleaned
by HEPA filters.

1300 Series A2 1-3Thermo Scientific
Section 1
Description
Safety lockout
To protect from UV radiation, the optional UV light will not turn on if
the front window is open. While the UV light is on, the front window is
closed and locked. The closed window blocks out the UV rays.
Airflow monitoring
Airflow monitoring determines the velocity of the airflow in the sample
chamber as well as the inflow velocity of air through the exhaust open-
ing. As soon as airflow velocities rise above or fall below a specified safety
value, an audible and a visual alarm is activated.
Window position monitoring
The position sensors detect the size of the front window opening and
indicate whether the window is open to the specified work position,
closed (energy saving) or in an unsafe intermediate position.
Thermo Performance Factor
The Thermo Performance Factor (PER) is a value that indicates the safe-
ty status of the cabinet. This value is calculated from data determined by
HEPA filter loading, airflow status and values captured during certifica-
tion. This data is entered into a parameter list of the control software
and interconnected. The result is indicated on the display by LEDs.
On certification by qualified personnel with a new HEPA filter, the per-
formance factor of the unit is indicated by green-green LEDs. As the
unit ages with use, the colors change. Red-red is unacceptable and the
unit should not be used. Any questions as to the safety of the unit
should be resolved before use.
Safety Systems
(continued)