Thermo Scientific HM340E User manual

Thermo Scientific
HM340E
Rotary Microtome
Product Code 905190
Operation Manual – English
ex Ser. No 44481
387863 Issue 8

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Thermo Scientific Rotary Microtome HM 340E
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Company Information
© Copyright 2016. Thermo Fisher Scientific Inc. All rights reserved.
Thermo Fisher Scientific Inc. (Thermo Fisher Scientific) is the world leader in serving science, offering a unique
combination of innovative technologies. Thermo Scientific is a brand name of Thermo Fisher Scientific.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific makes every attempt to ensure that the information contained in this supporting
document is correct and clearly stated, but does not accept responsibility for any errors or omissions. The
development of Thermo Scientific products and services is an ongoing process. Please ensure that any published
information you use as a reference is up to date and relates to the condition of the product. If necessary, check
with your local Thermo Fisher Scientific representative.
This document may not, in whole or in part, be copied, photocopied, reproduced, translated, or converted to any
electronic or other form without prior written consent of Thermo Fisher Scientific. All information contained in
this manual is proprietary and confidential, and the exclusive property of Thermo Fisher Scientific and is
protected by copyright.
Contact Addresses:
Thermo Shandon Limited (Trading as Thermo Fisher Scientific),
Tudor Road, Manor Park,
Runcorn, WA7 1TA, UK
Tel: [phone]; Fax: [phone]
Email: [e-mail]
Web: http://www.thermoscientific.com/pathology
USA Distributor:
Richard-Allan Scientific
Subsidiary of Thermo Fisher Scientific
4481 Campus drive
Kalamazoo
MI 49008
USA
This instrument conforms to the essential requirements of:
IVD Directive 98/79/EC
and its harmonized standards under Union harmonization legislation

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Symbols
The following symbols and conventions may be used throughout this document and on the instrument:
This symbol is used on the instrument, or in a document, to indicate that instructions must
be followed for safe and correct operation. If this symbol appears on the instrument, always
refer to the operator guide.
This symbol is used on the instrument, or in a document, to indicate that there are potential
biological risks associated with the instrument and / or instrument use. Always use Good
Laboratory Practice.
Cold surface, if necessary, use gloves
Cutting hazard, sharp edges, watch your fingers.
This symbol is used on the instrument, or in a document, to indicate that irritants or
potentially harmful chemicals are present. Refer to the Material Safety Data Sheets for the
products, and always use Good Laboratory Practice.
Separate taking back of electrical and electronic instruments in the countries of the European
Union:
This is to be applied in the countries of the European Union and other European countries
with a separate collecting system within the waste management. This product, being an
electro and/or electronic instrument, must be treated separately within the waste
management process (WEEE).
Manufacturer.
Serial number of the device. It is stated on the product label sticker.
It is built up as follows: SYYMMXXXX
S=Production site, Y=Year, M=Month, X= Counter
So the serial number states the production date of the device
A warning is given in the documentation if there is a danger of personal injury or damage to the
equipment or samples.
Note
Notes give additional information about a job or instruction, but do not form part of the instruction.

Thermo Scientific Rotary Microtome HM 340E
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Table of Contents
COMPANY INFORMATION .......................................................................................................................................... 3
SYMBOLS .................................................................................................................................................................... 4
TABLE OF CONTENTS .................................................................................................................................................. 5
CHAPTER 1 - SAFETY INFORMATION ........................................................................................................................... 7
GENERAL SAFETY................................................................................................................................................................. 7
CHEMICAL SAFETY ............................................................................................................................................................... 8
ENVIRONMENT ................................................................................................................................................................... 8
WARRANTY STATEMENT ....................................................................................................................................................... 8
EMC STATEMENT ............................................................................................................................................................... 9
CHAPTER 2 - INTRODUCTION .....................................................................................................................................10
INTENDED USE: ................................................................................................................................................................. 10
DESCRIPTION OF ROTARY MICROTOME HM 340E .................................................................................................................. 10
SCOPE OF DELIVERY STANDARD EQUIPMENT........................................................................................................................... 11
ADDITIONAL EQUIPMENT .................................................................................................................................................... 12
TECHNICAL DATA SHEET ..................................................................................................................................................... 14
CHAPTER 3 – OPERATING INSTRUCTIONS ..................................................................................................................16
SETTING UP THE MICROTOME.............................................................................................................................................. 16
FRONTAL VIEW ................................................................................................................................................................. 17
REAR VIEW ...................................................................................................................................................................... 17
LATERAL VIEW LEFT SIDE .................................................................................................................................................... 18
LATERAL VIEW RIGHT SIDE .................................................................................................................................................. 18
INITIAL START-UP .............................................................................................................................................................. 19
OPERATING PANEL ............................................................................................................................................................ 20
DISPLAY AND KEY FUNCTION ............................................................................................................................................... 21
Hand Wheel Brake ................................................................................................................................................... 21
Cutting Process Indication........................................................................................................................................ 22
Setting Section Thickness and Trimming Thickness.................................................................................................. 24
Cutting Movement and Retraction ......................................................................................................................... 26
Specimen Course Feed.............................................................................................................................................. 27
Factory Defaults ....................................................................................................................................................... 31
Memory Function..................................................................................................................................................... 31
Rocking Mode Function............................................................................................................................................ 32
ADAPTERS FOR SPECIMEN CLAMPING .................................................................................................................................... 33
Adapter, Non-Orienting ........................................................................................................................................... 33
Adapter, Orienting, Specimen Orientation............................................................................................................... 33
Changing and/or Fastening Specimen Clamps......................................................................................................... 34
Re-Adjusting Specimen Clamps ................................................................................................................................ 35
SPECIMEN CLAMPING......................................................................................................................................................... 36
Universal Cassette Clamp......................................................................................................................................... 36
Universal Cassette Clamp, Adjustable...................................................................................................................... 36
Standard Specimen Clamp ....................................................................................................................................... 37
Insert for Round Specimens, V-Insert and V-Distance Piece..................................................................................... 38
KNIFE AND BLADE CARRIERS ................................................................................................................................................ 41
Disposable Blade Carrier ER ..................................................................................................................................... 41
Disposable Blade Carrier E ....................................................................................................................................... 43
Knife Carrier C .......................................................................................................................................................... 45
Readjusting Knife or Blade Carriers.......................................................................................................................... 47
SECTION WASTE TRAY WITH INTEGRATED ARM REST ............................................................................................................... 48
LARGE FIELD MAGNIFIER .................................................................................................................................................... 48
Installing the Large Field Magnifier on the Microtone ............................................................................................. 49
CHAPTER 4 – WORKING WITH THE MICROTOME .......................................................................................................50