Thermo Scientific Dionex AS-DV User manual

Dionex AS-DV Autosampler
Operator's Manual
Document No. 065259
Revision 05
October 2012

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reproduction of the whole or any part of this document is strictly prohibited, except with
the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical
information in this document is for reference purposes only. System configurations and
specifications in this document supersede all previous information received by the
purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete,
accurate, or error-free and assumes no responsibility and will not be liable for any errors,
omissions, damage, or loss that might result from any use of this document, even if the
information in the document is followed properly.
Revision history: Revision 01 released April 2009
Revision 02 released July 2009
Revision 03 released May 2010
Revision 04 released December 2011
Revision 05 released October 2012
For Research Use Only. Not for use in diagnostic procedures.

Doc. 065259-05 10/12 i
1 • Introduction to the Dionex AS-DV. . . . . . . . . . . . . . . . . 1
1.1 Overview of the Dionex AS-DV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.3 Safety and Regulatory Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.3.1 Safety Messages and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.3.2 Safety Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.4 Deionized Water Requirements for IC . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.4.1 Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2•Description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Operating Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1.1 Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1.2 Front Interior Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1.3 Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2 Dionex AS-DV Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.2.1 Sampling Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.2.2 Rinsing Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.2.3 Optional High-Pressure Valve Operating Principles . . . . . . . . 15
2.3 Chromeleon Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.3.1 Software Control Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
2.3.2 System Wellness . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Contents

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3 • Operation and Maintenance . . . . . . . . . . . . . . . . . . . . . . . .21
3.1 Preparing the Sample Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
3.1.1 Cleaning the Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
3.1.2 Cleaning the Caps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
3.1.3 Cleaning the Filter Caps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
3.1.4 Filling the Vials and Installing the Caps . . . . . . . . . . . . . . . . . .23
3.2 Turning on the Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
3.3 Loading the Vial Carousel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
3.4 Connecting to Chromeleon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26
3.4.1 Connecting to Chromeleon 7 . . . . . . . . . . . . . . . . . . . . . . . . . . .26
3.4.2 Connecting to Chromeleon 6.8 . . . . . . . . . . . . . . . . . . . . . . . . .28
3.5 Creating a Chromeleon Instrument Method or Program . . . . . . . . . . . .30
3.5.1 Creating an Instrument or a Program with the Wizard . . . . . . .30
3.5.2 Dionex AS-DV General Parameters . . . . . . . . . . . . . . . . . . . . .32
3.5.3 Basic Edit Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
3.5.4 Advanced Edit Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
3.6 Creating a Chromeleon Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
3.7 Starting a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
3.8 Operating Details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
3.8.1 Interrupting the Autosampler . . . . . . . . . . . . . . . . . . . . . . . . . .38
3.8.2 Specifying a Bleed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
3.8.3 Specifying a Rinse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42
3.8.4 Returning to a Vial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
3.8.5 Replacing Vials During a Run . . . . . . . . . . . . . . . . . . . . . . . . . .47

Contents
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3.8.6 Inserting a Priority Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
3.8.7 Controlling the Delivery Volume . . . . . . . . . . . . . . . . . . . . . . . 49
3.8.8 Using Sample Overlap Mode . . . . . . . . . . . . . . . . . . . . . . . . . . 53
3.9 Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
3.9.1 Periodic Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
3.9.2 Annual Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
4 • Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
4.1 Alarms and Error Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
4.2 Liquid Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
4.3 Poor Peak Reproducibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
4.4 Unexpected or Extraneous Peaks Detected . . . . . . . . . . . . . . . . . . . . . . 66
4.5 Sample Not Loaded . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
4.6 Autosampler Stops Operating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
4.7 Excessive Backpressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
4.8 Autosampler Jammed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
5•Service. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
5.1 Replacing Tubing and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
5.2 Removing the Carousel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
5.3 Replacing the Sampling Tip and Tubing . . . . . . . . . . . . . . . . . . . . . . . . 74
5.4 Manually Raising the Sampling Head . . . . . . . . . . . . . . . . . . . . . . . . . . 77
5.5 Rebuilding a High-Pressure Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
5.6 Replacing a High-Pressure Valve Pod . . . . . . . . . . . . . . . . . . . . . . . . . 81

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5.7 Replacing the Main Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84
A • Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
A.1 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
A.2 Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
A.3 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
A.4 Sampling Mechanism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
A.5 Sample Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
B • Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
B.1 Facility Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
B.2 Unpacking the Dionex AS-DV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
B.3 Connecting the Dionex AS-DV Sample Out Line . . . . . . . . . . . . . . . . .88
B.3.1 Connecting the Sample Out Line Directly to an IC System. . . .89
B.3.2 Connecting the Sample Out Line to the Optional
High-Pressure Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90
B.4 Connecting the Drain Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94
B.5 Installing Chromeleon and the Chromeleon Software License . . . . . . .95
B.6 Connecting the Dionex AS-DV to the Chromeleon PC . . . . . . . . . . . . .95
B.7 Connecting the Power Cord . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96
B.8 Setting Up Chromeleon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
B.8.1 Installing the Dionex AS-DV Device Driver . . . . . . . . . . . . . . .97
B.8.2 Configuring the Dionex AS-DV in Chromeleon . . . . . . . . . . . .98
B.8.3 Connecting to the ePanel Set or Panel Tabset . . . . . . . . . . . .100

Contents
Doc. 065259-05 10/12 v
B.8.4 Verifying Chromeleon Communication . . . . . . . . . . . . . . . . . 102
B.8.5 Setting Up the Dionex AS-DV for Simultaneous Injections. . 102
C • Relay Control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
C.1 TTL Input Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
C.1.1 Load Cycle Timing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
C.1.2 Delivery Speed, Flush Factor, and TTL Homing
Delay Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
C.1.3 TTL Input Control Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
C.2 Connecting to the Relay Output and TTL Input . . . . . . . . . . . . . . . . . 108
C.3 Example Connections for Stand-Alone Operation . . . . . . . . . . . . . . . 109
C.4 Controlling the Relay Output . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
D • Reordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115
Index

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Doc. 065259-05 10/12 1
1 • Introduction to the Dionex AS-DV
1.1 Overview of the Dionex AS-DV
The Thermo Scientific Dionex™AS-DV Autosampler is a compact autosampler
that is capable of delivering between 0.1 mL and 5.0 mL of sample (in 0.1 mL
increments) to the sample loop or concentrator column in an ion chromatography
(IC) system. The Dionex AS-DV is compatible with the Thermo Scientific
Dionex ICS-5000, ICS-2100, ICS-1600, ICS-1100, and ICS-900 systems, as well
as legacy IC systems.
The Dionex AS-DV holds 50 vials (either 0.5 mL or 5.0 mL, or a combination of
the two sizes). Vials can be sampled in any order and multiple samples can be
taken from each vial. The autosampler remembers the vial size and volume
delivered for each vial position, allowing multiple samples to be taken from a vial
non-sequentially.
The unique Thermo Scientific Dionex PolyVial™sample vial incorporates a cap
that acts as a simple piston to force sample out of the vial. This eliminates the
need for an external sampling pump. Sample can be delivered to a sample loop or
a concentrator column with backpressures up to 690 kPa (100 psi). Caps with a
20 m filter are available to remove solid materials from the sample before
injection.
The Dionex AS-DV can be configured either without a valve (the standard
configuration) or with an optional 6-port high-pressure valve or 10-port high
pressure valve.
The Dionex AS-DV is controlled with a PC configured with Thermo Scientific
Dionex Chromeleon®7 Chromatography Data System (release 7.0 or later) or
Chromeleon 6.8 Chromatography Data System (release 6.80 SR6a or later). The
autosampler connects to the Chromeleon PC with a USB cable.
The Dionex AS-DVcan also be operated under relay control from a device
capable of executing timed events, such as a previous generation IC system
without USB connectivity.

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The Dionex AS-DVcan be ordered with the accessories needed for sampling from
either 0.5 mL vials or 5.0 mL vials:
•Order P/N 068908 to sample from 0.5 mL vials (Ship Kit P/N AAA-071115)
•Order P/N 068907 to sample from 5.0 mL vials (Ship Kit P/N 068887)
NOTE Vials must be ordered separately.
1.2 About This Manual
The electronic version (i.e., PDF file) of the Dionex AS-DVoperator’s manual
contains numerous links that you can click to take you to other locations within
the file. These links include:
•Table of contents entries
•Index entries
•Cross-references (underlined in blue) to sections, figures, tables, etc.
If you are not familiar with how to navigate PDF files, refer to the Help system for
Adobe®Acrobat® or Adobe Reader®for assistance.
Chapter 1,
Introduction
Introduces the Dionex AS-DV; explains the conventions
used in this manual, including safety-related information.
Chapter 2,
Description
Describes Dionex AS-DVoperating features and the
software required for autosampler control.
Chapter 3,
Operation and
Maintenance
Provides operating instructions for the Dionex AS-DV
and describes routine preventive maintenance procedures.
Chapter 4,
Troubleshooting
Lists problems and presents step-by-step procedures for
how to isolate and eliminate the cause of each problem.
Chapter 5,
Service
Provides step-by-step instructions for routine service and
parts replacement procedures that the user can perform.
Appendix A,
Specifications
Contains the Dionex AS-DVspecifications and facility
requirements.
Appendix B,
Installation
Provides specifications and installation site requirements
for the Dionex AS-DV.