Thermo Scientific BioCane CK50900 Series User manual

BioCane™
Cryobiological Storage Vessel
Type CK50900
Operation Manual and Parts List
LT509X17 Rev. 0
© 2010 Thermo Fisher Scientific
All rights reserved
Printed in U.S.A.

Thermo ScientificThermo Scientific
Manual Number LT509X17 (7000807)
0 -- 5/13/10 Transfer to Marietta (was LT509X17 8/12/09) ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Cryobiological Storage Vessel i
Catalog Number Model Number Name
CK509X2 807 BioCane 20
CK509X3 808 BioCane 34
CK509X4 809 BioCane 47
CK509X6 820 BioCane 73

Thermo Scientificii Cryobiological Storage Vessel Thermo Scientific
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.

Thermo Scientific Cryobiological Storage Vessel iiiThermo Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Extreme temperature hazards. Only qualified persons should perform procedures associated with this
symbol.
Potential biological hazards. Proper protective equipment and procedures must be used.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo products will be available at www.thermofisher.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Cryobiological Storage Vessel Thermo Scientific
Preface
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When more extensive service is necessary, we will assist you with direct factory trained
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Cryobiological Storage Vessel vThermo Scientific
Table of Contents
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Please Read . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Measuring Liquid Nitrogen Quantity . . . . . . . . . . . . . . . . . . . . . . . .3-2
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Section 1
Section 2
Section 3
Section 4
Section 5

Cryobiological Storage Vessel 1-1Thermo Scientific
Please Read
Section 1 Safety Information
This manual contains important operating and safety information. The
user must carefully read and understand the contents of this manual prior
to the use of this equipment.
Your Thermo Scientific cryogenic vessel has been designed with function,
reliability, and safety in mind. It is the user’s responsibility to install it in
conformance with local electrical codes. For safe operation, please pay
attention to the warnings and cautions throughout the manual.
All cryopreservation vessels, regardless of who manufactures them, use a
vacuum to provide for the super insulative properties needed to keep
nitrogen in a liquid form. They are very similar, in fact, to the thermos
vessels you may have used to store coffee, soup or milk.
Remember how fragile they were? Since no vacuum is perfect or will last
forever, we suggest that you monitor the consumption of liquid nitrogen
used by your vessel on a regular basis, i.e. every 1-2 days. By monitoring,
you may be able to anticipate subtle changes in consumption and possibly
react to problems before they arise.You may opt to use a level monitor for
this purpose.
Also, if your samples are super critical, one-of-a-kind, or irreplaceable,
consider allocating your samples into 2 separate vessels.
Remember the old adage about placing all your eggs into one basket…
Finally, because no vacuum will last forever, consider having your vessel
tested by a qualified cryovessel manufacturer every 7-10 years regardless of
any problems (or lack thereof) you may have had in the past. This will
help insure your samples against sudden loss of nitrogen due to vacuum
failure.

1-2 Cryobiological Storage Vessel Thermo Scientific
Section 1
Safety Information
Warnings
• Liquid nitrogen is extremely cold; it boils at -196°C.
• To avoid injury due to frostbite or ruptured vials use extreme care
whenever handling liquid nitrogen, liquid nitrogen storage or transfer
vessels or any objects which have come in contact with liquid
nitrogen.
• Leave no areas of skin exposed.
• Always wear proper safety attire over clothing: face shield, cryogenic
gloves, cryogenic apron.
• Do not tightly seal liquid nitrogen containers or prevent nitrogen gas
from escaping.
• Always handle vials with tongs.
• Use extreme care to prevent spilling and splashing liquid nitrogen
during transfer and removal of storage contents and holders.
• Immediately remove any clothing or safety attire on which liquid
nitrogen has been spilled or splashed .
• Get immediate medical attention for any frostbite injuries due to
liquid nitrogen.
• Never overfill liquid nitrogen vessels.
When inserting or removing cans, be careful not to come in contact
with the neck tube area of the vessel. Remove or insert cans slowly,
in a vertical manner. Scratches on the neck tube are can cause
premature vacuum failure.
Do not tamper with, or remove vacuum port (covered by black
plastic cap on side of vessel); this will void warranty.
Do not spill liquid nitrogen on vacuum port - this can cause vacuum
failure.
Always keep liquid nitrogen in an upright position.

Cryobiological Storage Vessel 2-1Thermo Scientific
Section 2 General Specifications
Declaration of Conformity
Copies of the Declaration of Conformity are available upon request.
Cryogenics Dewars (liquid nitrogen containers) meet the requirements of
Annex V Medical Devices Directive 93/42/EEC
EC Certificate - CE 00127
BioCane 20 BioCane 34 BioCane 47 BioCane 73
Height (Inches) 25.70" 26.60" 26.50" 27.00"
Diameter (Inches) 14.50" 18.20" 20.00" 22.00"
LN2 Capacity 20.50L 34.80L 47.40L 73.0L
Static Holding Time (Days) 227 193 121 121
Static LN2 Evaporation Rate 0.1L/day 0.18L/day 0.40L/day 0.60L/day
Liquid Phase Capacity 1.2 ml 180 vials 720 vials 1512 vials 2880 vials