Thermo Scientific TriPlus RSH User manual

Thermo Scientific
TriPlus RSH
Robotic Sample Handling
User Guide
P/N 31709620 Ninth Edition December 2015

© 2015 Thermo Fisher Scientific Inc. All rights reserved.
TriPlus RSH, TRACE 1300, TRACE 1310, TRACE GC Ultra and FOCUS GC are trademarks of Thermo
Fisher Scientific Inc., and its subsidiaries.
Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana 20090 Rodano-Milan, Italy
Tel: [phone]; Fax: [phone]
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-
free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Release history:
First edition, released September 2011 “Original Instructions”
Second edition, released October 2011; Third edition, released March 2012; Fourth edition, released
September 2012; Fifth edition, released March 2013; Sixth edition, released October 2013; Seventh Edition,
released June 2014; Eighth Edition, February 2015; Ninth Edition, December 2015
For Research Use Only. Not for use in diagnostic procedures.

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MY ORGANIZATION IS: (Check only one) MY PRIMARY APPLICATION IS: (Check only one)
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Editor, Technical Publications
Thermo Fisher Scientific S.p.A.
Strada Rivoltana km 4
20090 Rodano (MI)
Italy
Editor, Technical Publications
Thermo Fisher Scientific CMD GC-GC/MS
2215 Grand Avenue Parkway
Austin TX 78728-3812
Unites States of America

Declaration
Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
for the instrument safety, reliability and performance only if:
•installation
•re-calibration
•changesandrepairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
trained personnel
Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.
Thermo Fisher Scientific S.p.A.
Strada Rivoltana, 20090 Rodano - Milan - Italy — Tel: [phone] - Fax: [phone]
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations.
Thermo Fisher Scientific declares, under sole responsibility, that the product as originally delivered complies with
the requirements of the following applicable European Directives and carries the CE marking accordingly:
• Low Voltage Directive:2006/95/EC
• EMC Directive:2004/108/EC
• Machinery Directive: 2006/42/EC
… and conforms with the following product standards:
Safety
This device complies with:
• IEC61010-1:2010 3rd Edition | IEC/EN 61010-1 3rd Edition
• ANSI/UL 61010-1:2004 2nd Edition | CAN/CSA C22.2 No. 61010-1:2004 2nd Edition.
• EN 61010-2-010:2003 | EN 61010-2-051:2003 | EN 61010-2-081:2001+A1:2003 | EN 61010-2 101:2003
Electromagnetic Compatibility
This device complies with:
• IEC 61326-1:2nd Edition | EN 61326-1:2013 | CISPR 11:5th Edition
• EN 61000-6-2:2005 | IEC 61000-6-2:2nd Edition| IEC 61000-6-3:2nd Edition am1 | EN 61000-6-3:2007 +
A1:2011
• Conducted Emission, Subpart B. FCC part 15, §15.107(a) and §15.109(a)
Laser Class 1
• The selected Class 1 Laser for the TriPlus RSH module Barcode Reader complies with the following regula-
tions:

• 21 CFR1040.10 and 1040.11 except for deviations pursuant to Laser Notice No. 50, dated July 26, 2001
• EN60825-1:1994 + A1:2002 + A2:2001
• IEC60825-1:1993 + A1:1997 + A2:2001
FCC Compliance Statement
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher
Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/
or bulky for one person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Do not use radio frequency transmitters, such as mobile phones, in close proximity to the instrument.
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO THE
FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained
inside this manual pertaining to the safe use and operation of this product before using the device.

WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive
2012/19/EU. It is marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU)
Member State, and these companies should dispose of or recycle this product. See www.thermoscientific.com/rohsweee for
further information on Thermo Fisher Scientific’s compliance with these Directives and the recyclers in your country.
WEEE Konformität
Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2012/19/EU erfüllen. Das Produkt
ist durch folgendes Symbol gekennzeichnet:
Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten getroffen,
damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Mehr Information über die Einhaltung
dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise, die nützlich sind, um die
Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter www.thermoscientific.com/rohsweee.
Conformité DEEE
Ce produit doit être conforme à la directive européenne (2012/19/EU) des Déchets d'Equipements Electriques et
Electroniques (DEEE). Il est marqué par le symbole suivant:
Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union
européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage d'informations sur la conformité de Thermo
Fisher Scientific à ces directives, les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui
peuvent aider la détection des substances sujettes à la directive RoHS sont disponibles sur www.thermoscientific.com/rohsweee.

Conformità RAEE
Questo prodotto è marcato con il seguente simbolo in conformità alla direttiva europea 2012/19/EU (RAEE) sui rifiuti di
apparecchiature elettriche ed elettroniche:
Thermo Fisher Scientific si è accordata con una o più società di riciclaggio in ciascun Stato Membro della Unione Europea
(EU), e queste società dovranno smaltire o riciclare questo prodotto. Per maggiori informazioni vedere il sito
www.thermoscientific.com/rohsweee.
Conformidad RAEE
Este producto es marcado con el siguiente símbolo en conformidad a la Directiva 2012/19/EU de la Unión Europea sobre los
residuos de aparatos eléctricos y electrónicos:
Thermo Fisher Scientific ha contratado una o más empresas de reciclo para tratar residuos en cada Estado Miembro de la
Unión Europea, y estas empresas deberían reciclar o eliminar este producto. Referirse a www.thermoscientific.com/rohsweee
para una mayor información sobre la conformidad de Thermo Fisher Scientific con estas Directivas y para las empresas de
reciclaje en su país.
IMPORTANT
The symbol indicates the product must not be disposed of with the normal household wastes. Correct disposal of
this product prevents any potentially negative impact on the environmental and human health that could arise
from any inappropriate handling of the product itself.
WEEE and RoHS rules, while laid down at European level, are put into national law at national level. When ex-
porting to Europe, it is essential to comply with national law in each relevant country. The EU law simply serves
as a template for national laws, which may differ considerably.
Each EU Member State has own regulations regarding the application of these directives. Please refer to the
regulations in force in your country.

Thermo Scientific TriPlus RSH User Guide ix
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xv
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Power Rating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Safety Alerts and Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Instrument Markings and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Safety Information and Warnings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
General Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
Electrical Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxi
Laser Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxii
Other Hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxiii
Working with Toxic or other Harmful Compounds . . . . . . . . . . . . . . . . . . xxv
Biological Hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxv
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxvi
Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxvi
Chapter 1 Getting Familiar with Your TriPlus RSH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Instrument Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Definition of Terms, Naming Conventions, and Start Screen Icons . . . . . . . . . . 3
Definition of Terms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Naming Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Start Screen Icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
The TriPlus RSH System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Optional Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Power Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Contents

Contents
xTriPlus RSH User Guide Thermo Scientific
TriPlus RSH Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
TriPlus RSH for Liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
TriPlus RSH for Headspace. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
TriPlus RSH for SPME (Solid Phase Micro Extraction) . . . . . . . . . . . . . . . . 14
TriPlus RSH for ITEX (In-tube Extraction) . . . . . . . . . . . . . . . . . . . . . . . . . 15
Crossrails. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Crossrail X. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Crossrail Y. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
TriPlus RSH Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Tool Identification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
LS Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Syringes for LS Tools. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
HS Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Syringes for HS Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
SPME 1 and SPME 2 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Syringes for SPME Tools. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Definition of SPME Penetration Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
ITEX-2 Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Control Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Definitions of Active and Passive Hardware Modules . . . . . . . . . . . . . . . . . . . . 35
Active Versus Passive Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Active Versus Composite Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Modules Description and Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
TR Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
ATC Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Standard Wash Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Large Volume Wash Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Large Solvent Station. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Fast Wash Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Agitator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Vortexer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
MHE Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Naming Convention for MHE Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Principles of Multiple Headspace Extraction (MHE). . . . . . . . . . . . . . . . . . . 49
MHE Cycle Step-by-Step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Quantification from MHE Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . . 56
SPME Conditioning Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

Contents
Thermo Scientific TriPlus RSH User Guide xi
Barcode Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Safety Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Supported Types of Barcode Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Approved Barcode Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Barcode Label Positioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Reading the Barcode from a Vial or Well Plate . . . . . . . . . . . . . . . . . . . . . . . 67
Multidimensional Barcodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Barcode Label Size. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Barcode Reader Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Standard Tray Holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Liquid Cooled Tray Holder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Temperature Controlled Drawer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Temperature Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Temperature Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Temperature Stability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Condensation Build-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Safety Warning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Temperature Controlled Drawer Specifications . . . . . . . . . . . . . . . . . . . . . . . 74
Electronic Box for OC Injector Actuator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Handheld Controller. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Menu Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Menu Screen Status Bar Symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Function Keys and Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Scroll Wheel and Enter Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
Handheld Controller Versus the Virtual Handheld Controller . . . . . . . . . . . 79
Chapter 2 TriPlus RSH Basic Informations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
Starting the TriPlus RSH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
How to Start the TriPlus RSH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Short-cuts from Startup Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Access Levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
User Level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Extended User Level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
Service Level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Changing Access Level. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Example of Differences in Access Levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Setup, Installation, and Maintenance Menu Items. . . . . . . . . . . . . . . . . . . . . . . 90
Help Menu Item . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
About Menu Item . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Shutdown Menu Item . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Operation of the TriPlus System in Combination with Other
Chromatography Data Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Chapter 3 Setup Menu Item . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93
Starting Setup Menu Item. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Contents
xii TriPlus RSH User Guide Thermo Scientific
Setup Menu Item Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Agitator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Vortexer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Barcode Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Chromatograph System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
Input/Output Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Injectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
OC Actuator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
Motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Obstacle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Sample Drawers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
Tool Stations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
TR Station. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
ATC Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
Signals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Solvent Stations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
SPME Conditioning Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
Syringe Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Tool Adapters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
Naming Convention and Relationship between Software Classes‚ Tray
Holder, Trays, Tray Types, and Cap Type. . . . . . . . . . . . . . . . . . . . . . . . 141
Tray Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
Tray Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146
Trays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158
Vial Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 160
Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164
Wash Stations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 168
Tray Slots . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174
Unknown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174
Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174
Setup Menu Item - Set Date and Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 176
Setup Menu Item - Set TriPlus RSH Name . . . . . . . . . . . . . . . . . . . . . . . . . . 177
Setup Menu Item - Setup Network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178
Chapter 4 Maintenance Menu Items . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .181
Maintenance Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182
Check Tool Calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183
Check Teaching Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183
Check Configuration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 184
Reset Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 184
Create Diagnostic Backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
Create Configuration Backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186
Copy Backup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 187
Restore Backup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 188

Contents
Thermo Scientific TriPlus RSH User Guide xiii
Import Templates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 188
Chapter 5 Service Menu Item . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .189
Service Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 190
Prerequisites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 190
Installation Item . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191
Set Regional Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 193
Set Date and Time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 194
Set TriPlus RSH Name . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
Setup Network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
Calibrate Motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199
Teach Tool Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 202
Configure SPME Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 207
Set Reference Point . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209
Calibrate Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210
Setup Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 213
Teach Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 214
Check Teaching Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 218
Create a Backup File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 220
Create New Tray System Item. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 222
Teach Home Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 224
Changing Home Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 224
Chapter 6 Analytical Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .227
General Points Regarding Chromatographic Effects . . . . . . . . . . . . . . . . . . . . 228
Headspace Technique Points Regarding Chromatographic Effects . . . . . . . . . 230

Thermo Scientific TriPlus RSH User Guide xv
P
Preface
This manual describes the features and the components of the TriPlus RSH (Robotic Sample
Handling) integrated sampling system. Inside, you find all of the informations necessary for
the routine operations of your sampling system. This includes operating procedures, sample
injection techniques, diagrams, and the description of the major components.
This User Guide is intended for frequent or new TriPlus RSH users who are experienced at
using automated systems to run existing analytical methods.
This manual is organized as follows:
•Chapter 1, “Getting Familiar with Your TriPlus RSH,” provides informations to
familiarize with the TriPlus RSH.
•Chapter 2, “TriPlus RSH Basic Informations,” provides basic informations to start the
TriPlus RSH, and for using the Handheld Controller.
•Chapter 3, “Setup Menu Item,” describes the options of the Setup Menu operated either
with the Virtual Handheld Controller, or directly using the installed Handheld
Controller.
•Chapter 4, “Maintenance Menu Items,” describes the options of the Maintenance Menu
operated either with the Virtual Handheld Controller, or directly using the installed
Handheld Controller.
•Chapter 5, “Service Menu Item,” describes the options of the Service Menu operated
either with the Virtual Handheld Controller or directly using the installed Handheld
Controller.
•Chapter 6, “Analytical Troubleshooting,” gives a quick overview of possible causes and
recommended actions which can be taken to eliminate an erratic behavior.
Note The TriPlus RSH must be installed and set up properly before the Operating
Instructions can be used.

Preface
About Your System
xvi TriPlus RSH User Guide Thermo Scientific
About Your System
Thermo Fisher Scientific systems operate safely and reliably under carefully controlled
environmental conditions. If the equipment is used in a manner not specified by the
manufacturer, the protections provided by the equipment might be impaired. If you maintain
a system outside the specifications listed in this guide, failures of many types, including
personal injury or death, might occur. The repair of instrument failures caused by operation
in a manner not specified by the manufacturer is specifically excluded from the Standard
Warranty and service contract coverage.
Power Rating
Tr i P l us R S H
• 100/240 Vac +/-10%; 50/60 Hz; 5 A max; 200 VA (400 VA when two Power Modules
are required).
Detailed instrument specifications are in the Product Specification or Product Brochure.
Contacting Us
Thermo Fisher Scientific provides comprehensive technical assistance worldwide and is
dedicated to the quality of our customer relationships and services.
Use http://www.thermoscientific.com address for products information.address for products
information.
Use http://www.gc-gcms-customersupport.com/WebPage/Share/Default.aspx address to
contact your local Thermo Fisher Scientific office, or affiliate GC-GC/MS Customer
Support.
Related Documentation
In addition to this guide, Thermo Scientific provides the following documents for the TriPlus
RSH.
•TriPlus RSH Safety Guide, PN 31709600
•TriPlus RSH Preinstallation Requirements Guide, PN 31709610
•TriPlus RSH User Guide, PN 31709620
•TriPlus R SH Hardware Manual, PN 31709640
To suggest ways we can improve the documentation, follow this link to complete our Reader’s
Survey.

Preface
Safety Alerts and Important Information
Thermo Scientific TriPlus RSH User Guide xvii
Safety Alerts and Important Information
Make sure you follow the precautionary notices presented in this manual. The safety and
other special notices appear in boxes.
Special Notices
Notices includes the following:
Safety Symbols and Signal Words
All safety symbols are followed by WARNING or CAUTION, which indicates the degree of risk
for personal injury, instrument damage, or both. Cautions and warnings are following by a
descriptor, such as BURN HAZARD. A WARNING is intended to prevent improper actions that
could cause personal injury. Whereas, a CAUTION is intended to prevent improper actions
that might cause personal injury, instrument damage, or both. You can find the following
safety symbols on your instrument, or in this guide:
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Emphasizes important information about a task.
Tip Helpful information that can make a task easier.
Symbol Descriptor
BIOHAZARD: Indicates that a biohazard will, could, or might occur.
BURN HAZARD: Alerts you to the presence of a hot surface that could or might
cause burn injuries.
ELECTRICAL SHOCK HAZARD: Indicates that an electrical shock could or
might occur.
FIRE HAZARD: Indicates a risk of fire or flammability could or might occur.
EXPLOSION HAZARD. Indicates an explosion hazard. This symbol indicates
this risk could or might cause physical injury.

Preface
Safety Alerts and Important Information
xviii TriPlus RSH User Guide Thermo Scientific
FLAMMABLE GAS HAZARD. Alerts you to gases that are compressed,
liquefied or dissolved under pressure and can ignite on contact with an
ignition source. This symbol indicates this risk could or might cause physical
injury.
GLOVES REQUIRED: Indicates that you must wear gloves when performing a
task or physical injury could or might occur.
CLOTHING REQUIRED. Indicates that you should wear a work clothing when
performing a task or else physical injury could or might occur.
BOOTS REQUIRED. Indicates that you must wear boots when performing a
task or else physical injury could or might occur.
MATERIAL AND EYE HAZARD. Indicates you must wear eye protection when
performing a task.
HAND AND CHEMICAL HAZARD: Indicates that chemical damage or physical
injury could or might occur.
HARMFUL. Indicates that the presence of harmful material will, could, or
might occur.
INSTRUMENT DAMAGE: Indicates that damage to the instrument or
component might occur. This damage might not be covered under the
standard warranty.
LIFTING HAZARD. Indicates that a physical injury could or might occur if two
or more people do not lift an object.
MATERIAL AND EYE HAZARD: Indicates that eye damage could or might
occur.
READ MANUAL: Alerts you to carefully read your instrument’s
documentation to ensure your safety and the instrument’s operational ability.
Failing to carefully read the documentation could or might put you at risk for a
physical injury.
TOXIC SUBSTANCES HAZARD: Indicates that exposure to a toxic substance
could occur and that exposure could or might cause personal injury or death.
LASER HAZARD. Indicates that exposure to a laser beam will, could,or might
cause personal injury.

Preface
Instrument Markings and Symbols
Thermo Scientific TriPlus RSH User Guide xix
Instrument Markings and Symbols
Table 1 explains the symbols used on Thermo Fisher Scientific instruments. Only a few of
them are used on the TriPlus RSH. See the asterisk.
RADIOACTIVE HAZARD. Indicates that the presence of radioactive material
could or might occur.
For the prevention of personal injury, this general warning symbol precedes
the WARNING safety alert word and meets the ISO 3864-2 standard. In the
vocabulary of ANSI Z535 signs, this symbol indicates a possible personal
injury hazard exists if the instrument is improperly used or if unsafe actions
occur. This symbol and another appropriate safety symbol alerts you to an
imminent or potential hazard that could cause personal injury.
Table 1. Instrument Marking and Symbols (Sheet 1 of 2)
Symbol Description
Direct Current
*Alternating Current
Both direct and alternating current
Three-phase alternating current
Earth (ground) terminal
Protective conductor terminal
Frame or chassis terminal
Equipotentiality
*On (Supply)
*Off (Supply)
Equipment protected throughout by DOUBLE INSULATION or
REINFORCED INSULATION (Equivalent to Class II of IEC 536)
Fuse
*Instruction manual symbol affixed to product. Indicates that the you must
refer to the manual for specific WARNING or CAUTION information to
avoid personal injury or damage to the product.
3

Preface
Safety Information and Warnings
xx TriPlus RSH User Guide Thermo Scientific
Safety Information and Warnings
This safety guide raises awareness of potential safety issues and general points for
consideration for Thermo Fisher Scientific representatives during installation, and repair of
the TriPlus RSH, or parts of it (following the life cycle principle), as well as for the end user
TriPlus RSH in the lab during the learning phase, and in routine work.
General Considerations
• Before a unit is put to use, consult the TriPlus RSH User Guide and related documents
under all circumstances.
• Changes or modifications to this unit not expressly approved by the party responsible for
compliance, could void your’s authority to operate the equipment.
• Be aware that if the equipment is used in a manner not specified by the manufacturer, the
protective and safety features of the equipment might be impaired.
• The repair of instrument failures caused by operation in a manner not specified by the
manufacturer is expressly excluded from the standard warranty and service contract
coverage.
Caution, risk of electric shock
*Caution, hot surface
*Caution, biohazard
*Caution, Laser beam
*Symbol in compliance to the Directive 2012/19/EU on Waste Electrical
and Electronic Equipment (WEEE) placed on the European market after
August, 13, 2005.
Table 1. Instrument Marking and Symbols (Sheet 2 of 2)
Symbol Description
IMPORTANT Read this section first before operating the TriPlus RSH.