Thermo Scientific TriPlus RSH User manual

Thermo Scientific
TriPlus RSH
Robotic Sample Handling
User Guide
P/N 31709620 Ninth Edition December 2015

© 2015 Thermo Fisher Scientific Inc. All rights reserved.
TriPlus RSH, TRACE 1300, TRACE 1310, TRACE GC Ultra and FOCUS GC are trademarks of Thermo
Fisher Scientific Inc., and its subsidiaries.
Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana 20090 Rodano-Milan, Italy
Tel: [phone]; Fax: [phone]
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-
free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
Sale shall govern all conflicting information between the two documents.
Release history:
First edition, released September 2011 “Original Instructions”
Second edition, released October 2011; Third edition, released March 2012; Fourth edition, released
September 2012; Fifth edition, released March 2013; Sixth edition, released October 2013; Seventh Edition,
released June 2014; Eighth Edition, February 2015; Ninth Edition, December 2015
For Research Use Only. Not for use in diagnostic procedures.

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MY ORGANIZATION IS: (Check only one) MY PRIMARY APPLICATION IS: (Check only one)
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❏Government lab ❏Biomedical
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Editor, Technical Publications
Thermo Fisher Scientific S.p.A.
Strada Rivoltana km 4
20090 Rodano (MI)
Italy
Editor, Technical Publications
Thermo Fisher Scientific CMD GC-GC/MS
2215 Grand Avenue Parkway
Austin TX 78728-3812
Unites States of America

Declaration
Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
for the instrument safety, reliability and performance only if:
•installation
•re-calibration
•changesandrepairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
trained personnel
Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.
Thermo Fisher Scientific S.p.A.
Strada Rivoltana, 20090 Rodano - Milan - Italy — Tel: [phone] - Fax: [phone]
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations.
Thermo Fisher Scientific declares, under sole responsibility, that the product as originally delivered complies with
the requirements of the following applicable European Directives and carries the CE marking accordingly:
• Low Voltage Directive:2006/95/EC
• EMC Directive:2004/108/EC
• Machinery Directive: 2006/42/EC
… and conforms with the following product standards:
Safety
This device complies with:
• IEC61010-1:2010 3rd Edition | IEC/EN 61010-1 3rd Edition
• ANSI/UL 61010-1:2004 2nd Edition | CAN/CSA C22.2 No. 61010-1:2004 2nd Edition.
• EN 61010-2-010:2003 | EN 61010-2-051:2003 | EN 61010-2-081:2001+A1:2003 | EN 61010-2 101:2003
Electromagnetic Compatibility
This device complies with:
• IEC 61326-1:2nd Edition | EN 61326-1:2013 | CISPR 11:5th Edition
• EN 61000-6-2:2005 | IEC 61000-6-2:2nd Edition| IEC 61000-6-3:2nd Edition am1 | EN 61000-6-3:2007 +
A1:2011
• Conducted Emission, Subpart B. FCC part 15, §15.107(a) and §15.109(a)
Laser Class 1
• The selected Class 1 Laser for the TriPlus RSH module Barcode Reader complies with the following regula-
tions:

• 21 CFR1040.10 and 1040.11 except for deviations pursuant to Laser Notice No. 50, dated July 26, 2001
• EN60825-1:1994 + A1:2002 + A2:2001
• IEC60825-1:1993 + A1:1997 + A2:2001
FCC Compliance Statement
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher
Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/
or bulky for one person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Notice on the Susceptibility
to Electromagnetic Transmissions
Do not use radio frequency transmitters, such as mobile phones, in close proximity to the instrument.
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO THE
FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained
inside this manual pertaining to the safe use and operation of this product before using the device.

WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive
2012/19/EU. It is marked with the following symbol:
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU)
Member State, and these companies should dispose of or recycle this product. See www.thermoscientific.com/rohsweee for
further information on Thermo Fisher Scientific’s compliance with these Directives and the recyclers in your country.
WEEE Konformität
Dieses Produkt muss die EU Waste Electrical & Electronic Equipment (WEEE) Richtlinie 2012/19/EU erfüllen. Das Produkt
ist durch folgendes Symbol gekennzeichnet:
Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs-/Entsorgungsfirmen in allen EU-Mitgliedsstaaten getroffen,
damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann. Mehr Information über die Einhaltung
dieser Anweisungen durch Thermo Fisher Scientific, über die Verwerter, und weitere Hinweise, die nützlich sind, um die
Produkte zu identifizieren, die unter diese RoHS Anweisung fallen, finden sie unter www.thermoscientific.com/rohsweee.
Conformité DEEE
Ce produit doit être conforme à la directive européenne (2012/19/EU) des Déchets d'Equipements Electriques et
Electroniques (DEEE). Il est marqué par le symbole suivant:
Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union
européenne et ce produit devrait être collecté ou recyclé par celles-ci. Davantage d'informations sur la conformité de Thermo
Fisher Scientific à ces directives, les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui
peuvent aider la détection des substances sujettes à la directive RoHS sont disponibles sur www.thermoscientific.com/rohsweee.

Conformità RAEE
Questo prodotto è marcato con il seguente simbolo in conformità alla direttiva europea 2012/19/EU (RAEE) sui rifiuti di
apparecchiature elettriche ed elettroniche:
Thermo Fisher Scientific si è accordata con una o più società di riciclaggio in ciascun Stato Membro della Unione Europea
(EU), e queste società dovranno smaltire o riciclare questo prodotto. Per maggiori informazioni vedere il sito
www.thermoscientific.com/rohsweee.
Conformidad RAEE
Este producto es marcado con el siguiente símbolo en conformidad a la Directiva 2012/19/EU de la Unión Europea sobre los
residuos de aparatos eléctricos y electrónicos:
Thermo Fisher Scientific ha contratado una o más empresas de reciclo para tratar residuos en cada Estado Miembro de la
Unión Europea, y estas empresas deberían reciclar o eliminar este producto. Referirse a www.thermoscientific.com/rohsweee
para una mayor información sobre la conformidad de Thermo Fisher Scientific con estas Directivas y para las empresas de
reciclaje en su país.
IMPORTANT
The symbol indicates the product must not be disposed of with the normal household wastes. Correct disposal of
this product prevents any potentially negative impact on the environmental and human health that could arise
from any inappropriate handling of the product itself.
WEEE and RoHS rules, while laid down at European level, are put into national law at national level. When ex-
porting to Europe, it is essential to comply with national law in each relevant country. The EU law simply serves
as a template for national laws, which may differ considerably.
Each EU Member State has own regulations regarding the application of these directives. Please refer to the
regulations in force in your country.

Thermo Scientific TriPlus RSH User Guide ix
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xv
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Power Rating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Safety Alerts and Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Instrument Markings and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Safety Information and Warnings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
General Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
Electrical Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxi
Laser Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxii
Other Hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxiii
Working with Toxic or other Harmful Compounds . . . . . . . . . . . . . . . . . . xxv
Biological Hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxv
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxvi
Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxvi
Chapter 1 Getting Familiar with Your TriPlus RSH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Instrument Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Definition of Terms, Naming Conventions, and Start Screen Icons . . . . . . . . . . 3
Definition of Terms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Naming Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Start Screen Icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
The TriPlus RSH System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Sampling Unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Optional Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Power Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Contents

Contents
xTriPlus RSH User Guide Thermo Scientific
TriPlus RSH Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
TriPlus RSH for Liquids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
TriPlus RSH for Headspace. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
TriPlus RSH for SPME (Solid Phase Micro Extraction) . . . . . . . . . . . . . . . . 14
TriPlus RSH for ITEX (In-tube Extraction) . . . . . . . . . . . . . . . . . . . . . . . . . 15
Crossrails. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Crossrail X. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Crossrail Y. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
TriPlus RSH Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Tool Identification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
LS Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Syringes for LS Tools. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
HS Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Syringes for HS Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
SPME 1 and SPME 2 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Syringes for SPME Tools. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Definition of SPME Penetration Depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
ITEX-2 Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Control Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Definitions of Active and Passive Hardware Modules . . . . . . . . . . . . . . . . . . . . 35
Active Versus Passive Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Active Versus Composite Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Modules Description and Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
TR Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
ATC Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Standard Wash Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Large Volume Wash Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Large Solvent Station. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Fast Wash Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Agitator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Vortexer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
MHE Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Naming Convention for MHE Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Principles of Multiple Headspace Extraction (MHE). . . . . . . . . . . . . . . . . . . 49
MHE Cycle Step-by-Step . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Quantification from MHE Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . . 56
SPME Conditioning Station . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61