Thermo Scientific CRYO Plus 1 Operating instructions

Model 7400 Series
CRYO Plus 1, 2, 3, and 4
Liquid Nitrogen Storage System
Operating and Maintenance Manual 7007400 Rev. 11

Thermo ScientificThermo Scientific
MANUAL NO. 7007400
11 25763/fr-2099 10/29/09 Added vent port warnings and thawing notes (pgs 2-2 & 4-3) ccs
10 25127 12/18/08 Added detail to note in Specifications ccs
-- -- 4/16/07 Added hook-up/connection artwork in Section 1 ccs
9 23299 3/7/06 Added cautionary note concerning the use of Teflon tape on valves. ccs
Thread sealant is recommended (page 4-1).
-- -- 8/11/05 Clarified models shipped with lid strap ccs
-- -- 6/30/05 Added accessory list ccs
-- -- 4/8/05 Corrected control panel index numbers ccs
-- -- 12/2/04 Clarified electrical specifications ccs
8 -- 10/29/04 Corrected manual fill instructions, LED information ccs
7 21734/FR-1717 7/10/03 Updated alarm information ccs
6 20423/FR-1620 6/18/02 Added lid strap info, Section 1.4 ccs
Preface
7007400 Cryoplus i

Thermo Scientificii 7007400 Cryoplus Thermo Scientific
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. ▲
Caution All internal adjustments and maintenance must be performed by qualified service personnel. ▲
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Scientific makes no representations or warranties with respect to this manual. In
no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to the use of
this manual.
Note This equipment is Installation (Overvoltage) Category II, Pollution Degree 2. ▲
©2003 Thermo Scientific. All rights reserved.

Thermo Scientific 7007400 Cryoplus iiiThermo Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Extreme temperature hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Potential biological hazards. Proper protective equipment and procedures must be used.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Scientific
has contracted with one or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them. Further information on
Thermo’s compliance with this directive, the recyclers in your country and information on Thermo
products will be available at www.thermofisher.com.
Warning Whenever working with liquid nitrogen storage equipment in a closed environment, the use of personal
O2detection equipment is strongly recommended. ▲
✔Always use the proper protective equipment (clothing, gloves, goggles, etc.)
✔Always dissipate extreme cold or heat and wear protective clothing.
✔Always follow good hygiene practices.
✔Each individual is responsible for his or her own safety.

Thermo Scientificiv 7007400 Cryoplus Thermo Scientific
Preface
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7007400 Cryoplus vThermo Scientific
Table of Contents
Quick Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Connect Liquid Nitrogen Transfer Hose . . . . . . . . . . . . . . . . . . . . . .1-2
Attach Power Cord . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Connect Lid Strap (Model 7406/7407) . . . . . . . . . . . . . . . . . . . . . . .1-3
Connect to Electrical Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Install Platform Riser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Install Optional Temperature Sleeve . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Fill Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Remote Alarm Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
RS-232 Interface Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Tank Switcher Plug-in . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Installation Validation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Bar Graph . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Program Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Change High Level (Stop Filling) Setpoint . . . . . . . . . . . . . . . . . . .2-4
Change Low Level (Start Filling) Setpoint . . . . . . . . . . . . . . . . . . .2-4
Change High Temperature Alarm Setpoint . . . . . . . . . . . . . . . . . . .2-5
Set Microprocessor Internal Clock . . . . . . . . . . . . . . . . . . . . . . . . .2-5
The Optional Control Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-6
Troubleshooting the Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Section 1
Section 2
Section 3

vi 7007400 Cryoplus Thermo Scientific
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
General Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Defrosting the Storage Tank . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Defrosting the Vent Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Accessory List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Exceptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Inventory Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5
Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Handling Liquid Nitrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Table of Contents
Section 4
Section 5
Section 6
Section 8
Section 7
Appendix A

7007400 Cryoplus 1-1Thermo Scientific
Section 1 Quick Start-Up
Locate the storage container in a well ventilated area of the laboratory, with
adequate work space available for loading and unloading specimens. Allow
for adequate lid opening clearance.
This unit is designed to operate in the following environmental conditions:
Temperature: 5°C (41°F) to 40°C (104°F)
Humidity: 80% at <31°C, decreasing linearly to 50% at 40°C.
Altitude: < 2,000 meters (6,650 feet)
Warning Whenever working with liquid nitrogen storage equipment in a
closed environment, the use of personal O2detection devices is strongly
recommended. Refer to “Handling Liquid Nitrogen” in the appendix at
the end of this manual. ▲
Figure 1-1. Component Locations

1-2 7007400 Cryoplus Thermo Scientific
Section 1
Quick Start-Up
Attach Power Cord
The container should be located near the liquid nitrogen supply, allowing
enough space for nitrogen source tank replacement. Arrangements should
be made to collect the condensate, which will form on the transfer hose.
A four foot nitrogen transfer hose with a 1/2" flare fittings is supplied
with Model 7400/7401. A six foot hose is standard with Models
7402/7403, 7404/7405 and 7406/7407. The use of a transfer hose longer
than six feet may degrade system performance.
Caution The flair connection on the ends of the transfer hose does not
require any sealant. Pipe dope or sealing tape may cause contamination of
the liquid fill solenoid, or leaks at the hose connections. ▲
The storage system requires a user supplied low pressure regulated (22
PSIG) liquid nitrogen supply. Anything higher than 22 PSI will degrade
performance of the CryoPlus storage container.
Connect the transfer hose packed with the CryoPlus unit between the low
pressure, liquid outlet of the liquid nitrogen supply tank (22 PSIG) and
the storage container.
After the transfer hose has been connected, open the supply tank valve
and check the connections for leaks.
1. Loosen screw located on the power cord retainer. Spread the retainer.
2. Insert the power cord into the power outlet module. Tighten screw on
the power cord retainer.
3. Fit the power cord/outlet module assembly into the connection on the
unit. Tighten the module screws to secure the cord to the unit.
Connect Liquid
Nitrogen Transfer Hose
Figure 1-2. Power Cord

High LN Level Low LN Level
2
2
Vent - Do not connect or open
( 63.5) 25
7 (17.7) 6 (15.2) 5 (12.7) 4 (10.1)
3 (7.6) 2 (5.1)
(50.8) 20 (38.1) 15 (25.4) 10
(12.7) 5
Select
Scale
inches (centimeters)
Alarm indicator
2 inch to 7 inch low (vapor) scale
1 inch to 25 inch
high (liquid) scale
Choose high (liquid)
or low (vapor)
phase scale
Level
indicator
Level
Sensor
Fill
Valve
Bypass
Valve
Open
Valve
Bypass
Sensor
Temperature C
o
LN
Source
2
SilenceSilenceSilenceSilence
Lock
High
Level
High
Temp
Manual
Fill
Fill Valve
Open
Low
Level
Enter
Lock position of key switch
Programming access position of key switch
Fill Valve Open
indicator light
Manual Fill button
High Temp button
Low Level button
High Level
button
Down arrow
Up arrow
Enter button
Display
Open Valve fault indicator
LN Source fault indicator
2
Alarm indicator
Silence button
Bypass Valve fault indicator
Fill Valve fault indicator
Level Sensor fault indicator
Bypass Sensor fault indicator
Control Panel
No more than 6 ft
in distance
No sealer
Supply 22 PSI
Liquid side
Open valve after both ends of the
transfer hose are connected
No sealer
Storage
Platform (optional)
Vent - Do not block!