Thermo Scientific CRYO Plus 1 Operating instructions

Model 7400 Series
CRYO Plus 1, 2, 3, and 4
Liquid Nitrogen Storage System
Operating and Maintenance Manual 7007400 Rev. 11

Thermo ScientificThermo Scientific
MANUAL NO. 7007400
11 25763/fr-2099 10/29/09 Added vent port warnings and thawing notes (pgs 2-2 & 4-3) ccs
10 25127 12/18/08 Added detail to note in Specifications ccs
-- -- 4/16/07 Added hook-up/connection artwork in Section 1 ccs
9 23299 3/7/06 Added cautionary note concerning the use of Teflon tape on valves. ccs
Thread sealant is recommended (page 4-1).
-- -- 8/11/05 Clarified models shipped with lid strap ccs
-- -- 6/30/05 Added accessory list ccs
-- -- 4/8/05 Corrected control panel index numbers ccs
-- -- 12/2/04 Clarified electrical specifications ccs
8 -- 10/29/04 Corrected manual fill instructions, LED information ccs
7 21734/FR-1717 7/10/03 Updated alarm information ccs
6 20423/FR-1620 6/18/02 Added lid strap info, Section 1.4 ccs
Preface
7007400 Cryoplus i

Thermo Scientificii 7007400 Cryoplus Thermo Scientific
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. ▲
Caution All internal adjustments and maintenance must be performed by qualified service personnel. ▲
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Scientific makes no representations or warranties with respect to this manual. In
no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to the use of
this manual.
Note This equipment is Installation (Overvoltage) Category II, Pollution Degree 2. ▲
©2003 Thermo Scientific. All rights reserved.

Thermo Scientific 7007400 Cryoplus iiiThermo Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Extreme temperature hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Potential biological hazards. Proper protective equipment and procedures must be used.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Scientific
has contracted with one or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them. Further information on
Thermo’s compliance with this directive, the recyclers in your country and information on Thermo
products will be available at www.thermofisher.com.
Warning Whenever working with liquid nitrogen storage equipment in a closed environment, the use of personal
O2detection equipment is strongly recommended. ▲
✔Always use the proper protective equipment (clothing, gloves, goggles, etc.)
✔Always dissipate extreme cold or heat and wear protective clothing.
✔Always follow good hygiene practices.
✔Each individual is responsible for his or her own safety.

Thermo Scientificiv 7007400 Cryoplus Thermo Scientific
Preface
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7007400 Cryoplus vThermo Scientific
Table of Contents
Quick Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Connect Liquid Nitrogen Transfer Hose . . . . . . . . . . . . . . . . . . . . . .1-2
Attach Power Cord . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Connect Lid Strap (Model 7406/7407) . . . . . . . . . . . . . . . . . . . . . . .1-3
Connect to Electrical Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Install Platform Riser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Install Optional Temperature Sleeve . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Fill Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Remote Alarm Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
RS-232 Interface Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Tank Switcher Plug-in . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Installation Validation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Bar Graph . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Program Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Change High Level (Stop Filling) Setpoint . . . . . . . . . . . . . . . . . . .2-4
Change Low Level (Start Filling) Setpoint . . . . . . . . . . . . . . . . . . .2-4
Change High Temperature Alarm Setpoint . . . . . . . . . . . . . . . . . . .2-5
Set Microprocessor Internal Clock . . . . . . . . . . . . . . . . . . . . . . . . .2-5
The Optional Control Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-6
Troubleshooting the Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Section 1
Section 2
Section 3

vi 7007400 Cryoplus Thermo Scientific
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
General Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Defrosting the Storage Tank . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Defrosting the Vent Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Accessory List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Exceptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Inventory Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5
Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Handling Liquid Nitrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Table of Contents
Section 4
Section 5
Section 6
Section 8
Section 7
Appendix A

7007400 Cryoplus 1-1Thermo Scientific
Section 1 Quick Start-Up
Locate the storage container in a well ventilated area of the laboratory, with
adequate work space available for loading and unloading specimens. Allow
for adequate lid opening clearance.
This unit is designed to operate in the following environmental conditions:
Temperature: 5°C (41°F) to 40°C (104°F)
Humidity: 80% at <31°C, decreasing linearly to 50% at 40°C.
Altitude: < 2,000 meters (6,650 feet)
Warning Whenever working with liquid nitrogen storage equipment in a
closed environment, the use of personal O2detection devices is strongly
recommended. Refer to “Handling Liquid Nitrogen” in the appendix at
the end of this manual. ▲
Figure 1-1. Component Locations

1-2 7007400 Cryoplus Thermo Scientific
Section 1
Quick Start-Up
Attach Power Cord
The container should be located near the liquid nitrogen supply, allowing
enough space for nitrogen source tank replacement. Arrangements should
be made to collect the condensate, which will form on the transfer hose.
A four foot nitrogen transfer hose with a 1/2" flare fittings is supplied
with Model 7400/7401. A six foot hose is standard with Models
7402/7403, 7404/7405 and 7406/7407. The use of a transfer hose longer
than six feet may degrade system performance.
Caution The flair connection on the ends of the transfer hose does not
require any sealant. Pipe dope or sealing tape may cause contamination of
the liquid fill solenoid, or leaks at the hose connections. ▲
The storage system requires a user supplied low pressure regulated (22
PSIG) liquid nitrogen supply. Anything higher than 22 PSI will degrade
performance of the CryoPlus storage container.
Connect the transfer hose packed with the CryoPlus unit between the low
pressure, liquid outlet of the liquid nitrogen supply tank (22 PSIG) and
the storage container.
After the transfer hose has been connected, open the supply tank valve
and check the connections for leaks.
1. Loosen screw located on the power cord retainer. Spread the retainer.
2. Insert the power cord into the power outlet module. Tighten screw on
the power cord retainer.
3. Fit the power cord/outlet module assembly into the connection on the
unit. Tighten the module screws to secure the cord to the unit.
Connect Liquid
Nitrogen Transfer Hose
Figure 1-2. Power Cord

High LN Level Low LN Level
2
2
Vent - Do not connect or open
( 63.5) 25
7 (17.7) 6 (15.2) 5 (12.7) 4 (10.1)
3 (7.6) 2 (5.1)
(50.8) 20 (38.1) 15 (25.4) 10
(12.7) 5
Select
Scale
inches (centimeters)
Alarm indicator
2 inch to 7 inch low (vapor) scale
1 inch to 25 inch
high (liquid) scale
Choose high (liquid)
or low (vapor)
phase scale
Level
indicator
Level
Sensor
Fill
Valve
Bypass
Valve
Open
Valve
Bypass
Sensor
Temperature C
o
LN
Source
2
SilenceSilenceSilenceSilence
Lock
High
Level
High
Temp
Manual
Fill
Fill Valve
Open
Low
Level
Enter
Lock position of key switch
Programming access position of key switch
Fill Valve Open
indicator light
Manual Fill button
High Temp button
Low Level button
High Level
button
Down arrow
Up arrow
Enter button
Display
Open Valve fault indicator
LN Source fault indicator
2
Alarm indicator
Silence button
Bypass Valve fault indicator
Fill Valve fault indicator
Level Sensor fault indicator
Bypass Sensor fault indicator
Control Panel
No more than 6 ft
in distance
No sealer
Supply 22 PSI
Liquid side
Open valve after both ends of the
transfer hose are connected
No sealer
Storage
Platform (optional)
Vent - Do not block!

Connect to Electrical
Supply
Included with each unit is a lid strap for the user’s convenience.
1. Remove the protective white nylon screws from the areas indicated.
Discard these screws.
2. Install the strap as shown below, using the screws included with the
strap.
With the power switch turned OFF, connect the unit to a grounded
electrical outlet. See the data plate on the back of the unit, or the electrical
schematics included in this manual, for voltage and full load amps.
The power switch on the back of the unit is the mains disconnect and is
also a reset-type circuit breaker. If an overload condition occurs, the built-
in circuit breaker will trip and the power switch will turn off. Turning the
power switch on resets the circuit breaker. If the circuit breaker trips again
within a short time period, the unit should be checked by a qualified
electrician.
Warning Use only a grounded electrical receptacle. Failure to ground the
unit can result in serious injury. ▲
Figure 1-3. Lid Strap Installation
Connect Lid Strap
(Model 7406/7407)
1-4 7007400 Cryoplus Thermo Scientific
Section 1
Quick Start-Up

7007400 Cryoplus 1-5Thermo Scientific
Section 1
Quick Start-Up
Fill Unit
Install Optional
Temperature Sleeve
Depending on the inventory control system chosen, install the platform
riser (if required) as shown in Figure 1-4.
Note In the liquid phase, the standard platform remains in the bottom of
the tank. ▲
A Temperature Sleeve is designed to assist the temperature gradient within
the unit so that in a normal vapor phase setting of 3-5 inches of liquid, the
temperature under the lid remains below -130°C. The sleeve is standard on
all CryoPlus units and is installed when shipped from the factory.
When properly installed, the ends of the sleeve are aligned with the
temperature probe at the rear of the tank, and the square holes in the
bottom of the sleeve are aligned with the fill and pressure ports.
It is imperative that the positioning of the sleeve not block either the fill or
liquid level tube orifices located at the bottom rear of the tank. Should
blockage occur, it will cause filling and liquid level indicator problems.
When shipped from the factory, the liquid nitrogen level settings of all
CryoPlus units are set at Vapor Phase settings of five inches high limit,
three inches low limit (factory defaults). It is not recommended that these
settings be changed until the unit has been filled for the first time and
allowed to stabilize.
Caution The lid must remain open throughout the initial filling of the
storage container. ▲
Figure 1-4. Platform Riser
Install Platform Riser

Remote Alarm
Connector
When electric power and LN2have been connected, open the lid and turn
on the power switch to begin filling the unit. Because the unit must go
from ambient room temperature to -196°C, considerable boiling of the
liquid nitrogen takes place, turning into super-cooled nitrogen gas which
flows over the side of the open chamber. As this occurs, frost becomes
visible around the top of the unit. This is normal during the initial fill
with the lid open and disappears once the unit has stabilized.
As the unit fills, the bar graph on the front of the cabinet monitors the
progress by displaying the liquid level (green lights), the high and low limit
set points (flashing orange). Refer to Section 2. The storage container fills
until the liquid Nitrogen reaches the high level set point and the 5-inch
flashing orange LED changes to flashing red. The LN2storage container is
now in full automatic operation.
After the initial fill is complete, close the lid and allow the unit to stabilize
for 8 to 10 hours, or overnight, before changing the high or low level
setpoints or adding inventory.
Caution Some popping or cracking noises may be heard after the unit is
initially filled and the lid is opened and closed the first few times. This is
normal and quickly disappears. ▲
The CryoPlus control system provides remote alarm contacts, wired to an
RJ-11 connector on the back of the cabinet. Figure 1-4 identifies the pin
connections.
Fill (continued)
1-6 7007400 Cryoplus Thermo Scientific
Section 1
Quick Start-Up
Figure 1-4. RS232 and Remote Alarm Contact Connections
Figure 1-5. Alarm Contacts Junction Box Wiring

7007400 Cryoplus 1-7Thermo Scientific
Section 1
Quick Start-Up
Installation
Verification
Tank Switcher Plug-in
The CryoPlus storage system is equipped with an RS-232 Serial
Communication Interface for the remote transmission of data. An RJ-11
telephone style connector is located on the rear of the cabinet. A cable
with RJ-11 plugs and an RJ-11 to DB-25 adapter are required. Figure 1-4
identifies the pin connections.
The RS-232 provides information to a serial printer or terminal via the
following protocol:
9600 Baud
1 Stop Bit
No Parity
8 Data Bits
The power outlet on the back of the cabinet provides line voltage to a tank
switcher, if such equipment is used.
The following procedures test key elements of the CryoPlus Freezer system
and verify the unit's installation. These tests can be performed at the
operator's discretion. If any of these tests fail, contact the Technical Service
Department or your local Thermo sales representative.
LN2Supply
The source tank should indicate that it is full and the pressure to the
Cryo unit regulated at 22 PSIG. Check all connections.
Temperature Sleeve
When installed, the ends of the sleeve must align with the temperature
probe at the back of the tank and the pressure and fill ports are visible
through the square holes at the bottom of the sleeve.
Power Switch
Turn the unit on with the power switch located on the back of the unit.
Under normal conditions, all LEDs on the control panel and bar graph,
with the exception of the alarm LEDs illuminates for approximately 2-3
seconds. The power switch is the main disconnect for the system.
Keypad
Press each button on the control panel, listening for a "beep" response.
Control Panel Key Switch
Turn the key switch to the Programming Access position (.). Control
panel temperature display indicates alarm setpoint temperature. Turn the
key to Lock position and the display shows actual chamber temperature.
RS-232 Interface
Connector

Tank Switcher Outlet
Turn the key switch to Programming Access (.). Press Manual Fill while
checking the tank switcher outlet for line voltage with a volt meter.
When Manual Fill is pressed, liquid nitrogen begins to flow into the
tank. If the LN2level is more than one inch below high level setpoint,
liquid nitrogen will continue to flow after Manual Fill is released. If tank
level is less than one inch below high level setpoint, filling will stop
when Manual Fill is released.
LED Test
Turn the unit off. Turn the key switch to Programming Access and turn
the unit on while pressing and holding the Low Level button for four
seconds. Press Manual Fill to begin the test. Turn the unit off, when all
LEDs have cycled, to reset the system.
Remote Alarm Contacts
With the contacts wired to a remote alarm, turn the unit on, wait a few
seconds, then turn the unit off. The alarm should activate immediately.
Manual Fill
Turn the key switch to Programming Access. Press the Manual Fill but-
ton. The fill indicator will light and the unit will begin filling the cham-
ber. If the LN2level is more than one inch below the high level setpoint,
the liquid nitrogen will continue to flow after Manual Fill is released
until the high level setpoint is reached. To stop filling before high level,
turn the unit off, then on. If LN2is already in the tank and the level is
less than one inch below the high level setpoint, filling will stop when
Manual Fill is released.
Programming Access Test
With the unit turned on, turn the key to Programming Access. The con-
trol panel display will show the high temperature alarm setpoint.
Press High Level, then the up or down arrow. The setpoint moves
accordingly.
Press Low Level, then the up or down arrow. The setpoint moves accord-
ingly. Return the key to Lock. The control panel display shows chamber
temperature and bar graph shows new high and low level setpoints.
Note The unit returns to factory default settings unless setpoints were
saved in above tests by pressing Enter. ▲
1-8 7007400 Cryoplus Thermo Scientific
Section 1
Quick Start-Up
Installation
Verification (cont.)

7007400 Cryoplus 1-9Thermo Scientific
Section 1
Quick Start-Up
Dip Level Test
This test compares the liquid level shown on the bar graph with the
actual level in the tank, using the ruler supplied with the unit. With the
tank filled and stable, lower the ruler along the edge of the tank until it
is at the bottom. (Take subsequent measurements at this same location.)
When the LN2stops boiling, pull out the ruler. The actual liquid level
will be approximately one inch lower than the frost line on the ruler.
Compare this with the level shown on the bar graph. Measuring toler-
ance for the low scale (2" to 7") is ±1/2 inch. Measuring tolerance for
the high scale (1" to 25") is ±1 inch.
Caution Some shrinkage of the ruler may occur, depending on the level of
liquid nitrogen in the tank. ▲
Installation
Verification (cont.)

7007400 Cryoplus 2-1Thermo Scientific
Section 2 Operation
All functions of this CryoPlus
storage unit are controlled by
a programmable, state-of-the-
art microprocessor.
Commands to the control
system are given using the
control panel on the cabinet
lid (Figure 2-1). A multi-
colored bar graph on the front
of the cabinet shows the status
of the system, indicating the
liquid level inside the
chamber, and the high level
(stop fill) and low level (start
fill) points. See Figure 2-2.
Figure 2-1. Control Panel Elements
Figure 2-2. Level Indicators and Setpoints

2-2 7007400 Cryoplus Thermo Scientific
Section 2
Operation
The elements on the left side of the control panel illuminate when fault
conditions occur, providing information about the alarm state. Refer to
Section 3 of this manual.
A three element alarm bar illuminates during an alarm condition to
visually alert the operator. The Silence button is used to silence the audible
alarm. Refer to the alarm descriptions and corrective actions in Section 3
of this manual.
Other elements of the control panel are:
• A seven segment numeric display that shows the chamber temperature
directly under the lid when the keyswitch is in the Lock position.
When the key is in the Programming Access (.) position, the display
shows the High Temperature Alarm set point.
• The Enter button is used to send programming changes to the
microprocessor.
• Up and down arrows that change the high and low level settings when
making programming changes, are also used to change the high
temperature alarm setpoints.
• The High Level button changes the level at which the system stops
filling.
• Low Level is pressed to change the level at which the system starts
filling.
• The High Temp button changes the temperature at which the high
temperature alarm activates.
• Press Manual Fill to manually fill the tank. The level must be at least
1-1/4 inches below the high level setpoint to start a manual fill.
• Fill Valve Open light indicates that the fill valve is open.
• The key switch is used to allow program changes when the key is in
the (.) Programming Access position and to protect the system from
tampering or accidental button presses when the key is in the Lock
position.
• If the lid is opened frequently, condensation can occur on the vent
port, causing icing of the port. See the maintenance section for defrost
information.

Program Controller
7007400 Cryoplus 2-3Thermo Scientific
Section 2
Operation
Located on the front of the CryoPlus cabinet, the tri-color, 24 light bar
graph displays the liquid level inside the chamber, and (start fill) low level
and (stop fill) high level setpoints (Figure 2-2).
A Scale Select button at the base of the bar graph toggles the scale between
the high (liquid phase) and low (vapor phase) scales. Refer to Figure 2-2.
Numbers in parenthesis are the metric equivalents.
The left side scale is for settings from 1-inch to 25-inches in 1 inch
increments.
The right side scale is for settings from 2-inches to 7-inches in 1/4"
increments (vapor scale). The three light bars at the top of the level panel
are visible alarm lights that coincide with the alarms indicated on the top
control panel on the lid.
The three colors of the bar graph (Figure 2-3) are:
Orange (steady) indicates the remaining space
above the high level setpoint.
Orange (flashing): Under normal conditions,
the flashing upper LED is the high level (stop
fill) setpoint, and the flashing lower LED is the
low level (start fill) setpoint.
Green indicates the actual liquid Nitrogen
level.
Red (steady) indicates the amount of space
below or above setpoint from the actual liquid
level.
Red (flashing) indicates that the liquid level
is above or below the level set points. A
possible alarm condition is pending.
Single Red (flashing) after a fill operation indicates that the liquid level is
at the high level setpoint. This is not an alarm condition.
Caution The Key Switch must be in the Programming Access (.) position
to program the controller or to access any Touch Pad function. The
Controller will automatically return to the lock position, even though the
key is in the Access (.) Position, if no entry is made on the key pad within
four (4) minutes. On the high scale (left side, 2"- 25"), the high and low
level setpoints must be at least 3" apart. On the low scale (right side, 1.5"-
7.25"), the high and low level setpoints must be at least 1.25" apart. ▲
Figure 2-3. Bar Graph Colors
Bar Graph