Thermo Scientific Thermo Scientific Sorvall Legend Micro 17 User manual

Thermo Scientific
Sorvall Legend Micro 17 / 17R 21 / 21R
Centrifuge
Instruction Manual
50156058-c • 03 / 2020
Visit us online to register your warranty:
thermofisher.com/labwarranty

How to use this manual
Use this manual to get acquainted with your
centrifuge and its accessories.
The manual helps you to avoid inappropriate handling.
Make sure to keep it always close to the centrifuge.
A manual that is not kept available cannot provide
protection against improper handling and thus
against damage to persons and objects.
This manual comprises chapters on
xSafety regulations
xInstrument description
xTransportation and hook-up the centrifuge
xRotor program and accessories
xUse of the centrifuge
xMaintenance and care
xTroubleshooting
xTechnical data
xIndex
Please fold out
Overleaf you will find a graphic illustra-
tion of the control panel with a survey of
the most important functions

rotor turns
rpm/RCF
switch key
display
run time
quick run
open lid
(only for instruments with refri
g
eration unit)
temperature
speed/RCF
stop
start
“set”keys“set”keys
pre-temperature-regulation
(only for instruments with refriger
a
tion unit)
Before switching on the centrifuge
please read this manual!
Note that “min-1” stands for “rpm” and
“x g” stands for “RCF”

Control panel
Display panels
Speed / RCF
Resting state: current value (0) or pre-selected set-point value
During run: present current value of speed or RCF
(after activation of switch key)
Circulating light-points:
rotor turns
End: „End“
Error codes: flashing display with error code
Run time
Resting/End: current value (0) / “End”, or pre-selected set
point value
(in minutes, or "hd" for permanent operation)
Time selection/run: - remaining time till “0” in minutes
Permanent operation (hd): - run time passed in seconds/ minutes
„Quick run“: - run time passed in seconds/ minutes
Temperature*
according to: current sample temperature in °C
(in temperature equilibrium)
* only for instruments with refrigeration unit
Keys
start: start of the centrifuge
stop: manually stop of a run
open lid: open lid
(possible only with the instrument switched on and
standstill of the rotor)
quick run: short-term run up of the centrifuge as long as key
remains pressed
Switch
Speed/RCF: switching between speed and RCF display
Pre-temp: pre-temp-function*
“set”keys: stepwise increase/decrease of set-point values
Short pressing of any of the “set”keys:
switch from current to pre-selected set-point value, signaled by
flashing display.
(Error codes (see “troubleshooting” section):
E-14: over temperature centrifuge chamber (> 50 °C)
E-22: troubled speed measurement
E-24: lid will not open
E-31: over temperature motor
E-36: over amperage or over voltage
E-46: lid was opened manually during run
E-57: imbalance
E-60: under temperature centrifuge chamber (< -20 °C)

Contents
1
Contents
For your safety............................................ 3
Safety instructions in this manual............................. 3
Proper use................................................................ 4
Improper use............................................................ 4
Centrifuging hazardous materials ............................ 4
Handling the centrifuge ............................................ 4
Conformity to current standards............................... 5
Description of the instrument.................... 7
Pieces delivered....................................................... 7
Safety systems......................................................... 7
Properties................................................................. 8
Before use................................................... 9
Where to install the centrifuge.................................. 9
Transport and installation the centrifuge.................. 9
Centrifuge with refrigeration unit .......................... 9
Mains connection ................................................... 10
Removing the transport protection......................... 10
Accessories .............................................. 11
Rotors for the LEGEND MICRO 17............................ 12
Rotors for the LEGEND MICRO 21............................ 14
Rotors for the LEGEND MICRO 17R ......................... 16
Rotors for the LEGEND MICRO 21R ......................... 18
Adapter................................................................... 20
Handling the rotor .................................... 21
Rotor lid with a spring lock .................................21
Operation without rotor lid ..................................22
Rotor lid with screw plug..................................... 23
Aerosol-tight application.........................................24
Checking for aerosol-tightness...........................26
Operation .................................................. 27
Switching on the centrifuge....................................27
Opening the lid.......................................................27
Closing the lid.........................................................27
Installing the rotor...................................................28
Loading the rotor....................................................30
Maximum load .................................................... 30
Filling the centrifuge tubes .................................30
Placing the tubes in the rotor.............................. 31
Entering parameters...............................................32
Switching from speed to RCF display....................32
Selecting speed...................................................... 32
Entering the RCF value.......................................... 33
Concerning the RCF value.................................33
Selecting the run time ............................................34
Run time selection.............................................. 34
Continuous operation ......................................... 34
Setting the temperature..........................................35

Contents
2
Pretemp..............................................................36
Starting the centrifuge............................................37
Changing the settings during the run.....................37
Stopping the centrifuge ..........................................38
Preset run time ...................................................38
Continuous operation .........................................38
Short-time centrifugation........................................38
Removing the rotor.................................................39
Audible alarm .........................................................39
Turning off the centrifuge .......................................40
Maintenance and care ..............................41
Maintenance to be performed by the customer .....41
Cleaning..............................................................41
Cleaning the filter unit.........................................42
Disinfection.........................................................43
Decontamination.................................................45
Autoclaving.........................................................45
The Thermo Service offer.......................................46
Warranty conditions................................................46
Troubleshooting .......................................47
Mechanical emergency lid release.........................47
Problems you can handle yourself.........................49
Contacting Service .................................................55
Technical data...........................................57
Component parts....................................................57
The "Easycontrol" user interface............................58
Performance...........................................................59
Electrical connections.............................................61
Appendix ...................................................63
Speed / RCF diagram.............................................65
Autoclaving protocol...............................................71
Index ..........................................................73

For your safety
3
For your safety
Safety instructions in this manual
This symbol denotes potential hazards
to persons.
This symbol denotes potential damage
to the centrifuge or parts in its imme-
diate surroundings.
Warning: hot surfaces
General danger area
Before switching on the centrifuge please
read this manual!
General hints are marked with this
symbol.
Sorvall®centrifuges are manufactured according to
current technical standards and regulations. Nonethe-
less, centrifuges may pose danger to individuals and
surrounding if
xthey are not used as designed
xthey are operated by untrained personnel
xtheir design is improperly changed
xthe safety instructions are not followed
Therefore, personnel involved with oper-
ation and maintenance of the centrifuge
must read and follow the safety instruc-
tions.
In addition, the pertinent regulations for prevention of
accidents must be strictly followed.
This manual is an integral part of the centrifuge as-
sembly and must be kept close at hand at all times.
When damages to the power cord or at
casing are noticed the centrifuge must
be set out of operation!

For your safety
4
Proper use
This centrifuge is designed to separate sample
mixtures of different densities like chemicals,
environmental samples and other non-human body
samples.
Improper use
During a run, a safety zone of 30 cm around the centri-
fuge must be maintained where neither persons nor
hazardous materials may be present.
The centrifuge may cause harm to its user or other
persons or may damage goods if safety measures are
not followed:
Centrifuging hazardous materials
xThe centrifuge is neither made inert, nor is it explo-
sion-proof. Therefore never use the centrifuge in an
explosion-prone environment.
xDo not centrifuge explosive or flammable substanc-
es. The same holds for substances prone to react
violently with each other.
xDo not centrifuge toxic or radioactive substances or
pathogenic Micro-organisms without suitable safety
systems.
If Microbiological samples of risk group II (accord-
ing to "Laboratory Bio-safety Manual" of WHO) are
being centrifuged, aerosol-tight bio-seals have to be
used.
For materials in a higher risk group, more than one
precaution is required.
xShould toxins or pathogenic substances enter the
centrifuge or its parts, you must perform appropri-
ate procedures for disinfection (see "Maintenance
and care –Disinfection").
xStrongly corrosive substances that may cause
damage to materials and reduce the mechanical
strength of the rotor may be centrifuged only inside
protective tubes.
Handling the centrifuge
xUse only original accessories for the centrifuge.
The only exceptions are common glass or plastic
centrifuge tubes if they are approved for the rotor
speed and RCF values.
xNever use the centrifuge unless the rotor is properly
installed.
xYou may use the centrifuge only with a properly
loaded rotor. You must not overload the rotor.
xStrictly follow the rules and regulations for cleaning
and disinfection.
xIf the rotor or the rotor lid shows signs of corrosion
or wear, you must stop using it.

For your safety
5
xNever open the lid manually if the rotor is still turn-
ing.
xYou may use the emergency lid release only in
case of emergency, e.g. during aninterruption of
power supply
(see chapter “Troubleshooting”).
xNever use the centrifuge with an opened lid.
xNever use the centrifuge if the front panel has been
partially or totally removed.
xChanges in mechanical or electrical components of
the centrifuge may only be carried out by individu-
als authorized by Thermo.

For your safety
6
for your notes