Thermo Scientific Matrix 1029 User manual

Thermo Scientific
Matrix Electronic Pipette
User Manual

Manual N09238
Thermo Scientific Matrix
Electronic Pipette
User Manual

ii
CONTENTS
Congratulations! ............................................................................................. 1
Before You Begin............................................................................................. 1
A. INTRODUCTION ....................................................................................... 2
 A.1ThermoScienticMatrixSingleChannelPipette ................................................3
 A.2ThermoScienticMatrixMultichannelPipette ....................................................3
B. PIPETTE OVERVIEW................................................................................. 4
 B.1Components........................................................................................................4
 B.2GeneralDescription ............................................................................................5
 B.3Keypad ................................................................................................................6
 B.3.1OperationKeys ................................................................................................6
 B.3.2ProgrammingKeys...........................................................................................7
 B.3.3SpecialFunctionKey .......................................................................................8
 B.4Display ..............................................................................................................10
 B.5PipetteSpecications........................................................................................11
 B.6HazardsandPrecautions..................................................................................12
C. PROGRAMMING AND OPERATION....................................................... 13
 C.1ProgrammingtheElectronicPipette .................................................................13
 C.1.1OpenProgrammingModeor“ScratchPad”(P-0) .........................................13
 C.1.2EnteringaPrograminOpenProgrammingMode..........................................13
 C.1.3StoringaProgram..........................................................................................16
 C.1.4ProgramRecall ..............................................................................................17
 C.1.5AdjustingPipettingSpeed ..............................................................................17
 C.1.6ReviewingtheProgram..................................................................................17
 C.2ProgrammingExamples....................................................................................18
 C.2.1SampleTransfers...........................................................................................18
 C.2.2IncrementalPipetting .....................................................................................19
C.2.3 Serial Dilutions ...............................................................................................19
 C.2.4SimpleDilutions .............................................................................................20
 C.2.5SupernatantCollection...................................................................................20
 C.3OperatingProcedures.......................................................................................21

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D. CALIBRATION ......................................................................................... 23
 D.1VolumeMeasurement .......................................................................................23
 D.2PipetteCalibration.............................................................................................24
 D.2.1FactoryCalibrationStep ................................................................................24
 D.2.2“EASY”CalMode...........................................................................................24
 D.2.3“CALC”Mode .................................................................................................25
 D.2.4CALVIEW .......................................................................................................27
 D.2.5CALRESET ....................................................................................................27
 D.3RecommendationsforAccuratePipetting.........................................................27
E. TROUBLESHOOTING.............................................................................. 29
F. MAINTENANCE ........................................................................................ 31
 F.1SingleChannel Maintenance.............................................................................32
 F.1.1SingleChannel Disassembly ..........................................................................32
 F.1.2SingleChannelCleaningandAutoclaving......................................................34
 F.1.3PipetteReassembly........................................................................................34
 F.2Multichannel Maintenance .................................................................................35
F.2.1Multichannel Disassembly ..............................................................................35
F.2.2MultichannelCleaningandAutoclaving ..........................................................38
 F.2.3MultichannelMaintenanceandReassembly ..................................................38
 F.3Installing/ReplacingBatteries ............................................................................44
 F.3.1Installing/ReplacingNiMHBatteries ...............................................................44
 F.3.2Installing/ReplacingLithium-IonBattery(16Ch) ............................................48
 F.4Charging/RechargingBatteries..........................................................................50
 F.4.1Charging/RechargingNiMHBatteries.............................................................50
 F.4.2Charging/RechargingLithium-IonBattery(16Ch)..........................................51
G. TECHNICAL SERVICE ............................................................................ 53
 G.1InstrumentWarranty .........................................................................................53
 G.2ExpertPipetteService ......................................................................................54
 G.3OnSitePipetteServiceOptions .......................................................................55
 G.4RecommendedEquipmentDecontaminationMethods ....................................56
 G.5TipsandReservoirs ..........................................................................................58

1
Congratulations!
Youhavepurchasedthestate-of-the-artinprecisionpipetting.TheThermo
ScienticMatrixElectronicPipetteistherstcordlesselectronicpipettethat
“understands”themyriadofliquidhandlingprotocolstypicallyperformedin
today’slaboratory.
Ergonomicallydesignedandbalancedtoreducefatigue,theMatrixelectronic
pipettefunctionssointuitively,youmayneedonlyaquicksurveyofthis
manual to use it.
Theelectronicpipetteexecutesyourliquidpipettingtasksquickly,accurately,
andwithlessriskofdevelopingrepetitivestraininjuries(RSI)typically
associatedwithmanualpipetteuse.Laboratoryresultswillbecomemore
standardized,whilelesstimeisexpendedontediousbenchprocedures.
Before You Begin
BeforeusingtheMatrixelectronicpipette,verifythecontentsofthepackage.
Thepackageshouldcontainthefollowingitems:
1ElectronicPipette
1Operator’sManual
2RechargeableNiMHBatteriesor1Lithium-IonBattery(16 Channels)
1ACPowerTransformer
1WarrantyCard
1Accuracy/PrecisionDataSheet
Ifitemsaremissing,contactaThermoFisherScienticrepresentativeor
contactcustomerserviceat 800-345-0206.
Note: Batteries must be installed and recharged before using the Matrix
electronic pipette Refer to the following sections in this manual:
Section F.3, Installing/Replacing Batteries
Section F.4, Charging/Recharging Batteries

2
A. INTRODUCTION
TheThermoScienticMatrixElectronicPipetteprovidestheconvenienceof
amanualpipettor,withtheprogrammingcapabilitiesofanelectronicpipette.
TheMatrixelectronicpipettefeaturesinclude:
•Expandedmemoryforstoringuptovepipettingprograms.
•Eachprogramcanholdamaximumof40steps.
•“ScratchPad”programmingmodewhichallowsforfastandsimple
programchangesthatarenotstoredintomemory.
•Enhancedspeedcontrolofferssloweraspiratinganddispensingof
viscousliquids.Ideal for gel loading!
•“EASY”CALwhichsimpliesin-labcalibrationvericationusing
distilledwater.
TheMatrixelectronicpipetteislightweightandcordless,utilizingrechargeable
batteries,thatcanbeusedduringrecharge(with the exception of single
channel pipettes).Thepipettealsohasanautomaticshut-offfeaturetoextend
batterylife.
Theelectronicpipetteusesintegratedcircuittechnologytostoreprogramming
information.Itwillholdupto40stepsinaprogram,withupto5programs
savedinmemory,and1scratchpad(See section C.1.1 Open Programming
Mode or “Scratch Pad”).Theprograminformationisretainedinmemoryeven
ifthebatteriesarefullydischargedorremoved.
Theelectronicpipette usesa“paceddispense”featurethatperformsrepeat
pipettingstepswhilethetriggerisdepressed.

3
Introduction
A.1 Thermo Scientic Matrix Single Channel Pipettes
Thesinglechannelelectronicpipetteareavailableinanumberofmodel
congurations(see Figures A-1)from12.5µlto1250µl,ranginginvolume
from0.5µlto1250µl.Eachpipetteiscolor-codedforeasyvolume
identication(see section B.5, Pipette Specications).Twomodels,250µland
1250µl,areofferedwithashorterejectorsleevereducingtheoveralllength
comparedtothestandardpipette.Idealforuseinconnedspacessuchas
biosafetycabinet
A.2 Thermo Scientic Matrix Multichannel Electronic Pipettes
Themultichannelelectronicpipettesareavailableinanumberof
congurationsfrom8to16channnels.AnexpandableEXPpipetteis
alsoavailablein6-channelandcustomcongurations.Modelsrangein
volumefrom0.5µlto1250µl.Eachpipetteiscolor-codedforeasyvolume
identication(see section B.5, Pipete Specications).TheThermoScientic
MatrixEqualizer®pipettehasequaltipspacingbetween4.5mmand14.15
mm(depending on model).
6-Channel
Expandable
8-Channel 12-Channel 16-Channel
384Model
1250µl 250µl 125µl 30µl 12.5µl
1250µl
Shorty
250µl
Shorty
Figure A.1: Single Channel Models
Figure A.2: Multichannel Models

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Pipette Overview
B. PIPETTE OVERVIEW
B.1 Components
Figure B.1b: Multichannel Components
Figure B.1a:
Single Channel Components

5
Pipette Overview
B.2 General Description
Keypad.Thekeypadcontainstheoperationkeysthatareusedin
programmingthepipettingsequenceandvolumes.
(See Section B.3, Keypad).
Display.ThedisplayisanLCDscreenthatshowsthepipettingoperation
(ll, dispense, mix, or purge)anduidvolumeforeachstepinthepipetting
program.(See Section B.4, Display).
Battery Case.Thebatterycasecontainstherechargeablebatteries.Nickel
MetalHydridebatteriesfullychargein14-16hours,whileLithium-Ionbatteries
fullychargein2hours.Thepipettecanperformafullday’sworkloadwithno
needtorecharge.Batterylifeisextendedwithautomaticshut-offafter
10minutesofinactivity.(See Section F.3, Installing/Replacing Batteries).
Pipetting Trigger.Thepipettingtriggerinitiatesaprogram.Asinglepipetting
stepisexecutedbyaquickpressandreleaseofthetrigger.Repeatpipetting
isexecutedbypressingandholdingthetrigger.
Note: At the end of any dispense, the pipette tips should be removed from the
uid before releasing the trigger, to prevent aspiration of uid.
Ejector Trigger.Theejectortriggerispressedtoejectthetips
fromthepipette.
Tip Fittings.Thetipttingsformanairtightsealwiththetipstoensure
pipettingaccuracy.
Note: Electronic pipettes are equipped with chemically-resistant (PEEK) tip
ttings (and ejector sleeves on single channel models) that allow pipetting of
acidic solutions without damage to the tip ttings.
Color-coded Ring.Theringaroundthepipettehandleiscolorcoded
toidentifythepipettingvolumeofthehandle(See Section B.5, Pipette
Specications).
Transformer Connector.Thetransformerconnectorreceivestheplugfrom
thepowertransformerforrechargingthebatteries.Thepipettecanbeused
whilerecharging(with the exception of single channel pipettes).

6
Pipette Overview
B.3 Keypad
Thekeypadcontainstheoperationkeys,programmingkeys,andthespecial
functionkey.
Figure B.3: Electronic Pipette Keypad
B.3.1 Operation Keys
[On/Off] TheOn/Offkeyturnsthepipetteonoroff.Whenthepipetteis
turnedon,itemitsabeeptone,ashes“ZERO”onthedisplay,
thenautomaticallyresetsthepistonstozeroposition.After
zeroing,thedisplayshowstherststepoftheprograminthe
RUNmode.
Note: If the pipette is being programmed for the rst time, the
rst step will be the ll operation, with the default ll volume. (See
Section C.1, Programming the pipette, for the default settings).
[Prog] TheProgramkeyontheMatrixelectronicpipetteenablesyouto
storeuptoveseparateprogramsinmemoryforfuturerecall.
Eachprogramcanholdupto40steps.Programsarestoredin
reservedprogramlocationnumbers1–5.Program“0”isusedas
a“scratchpad”wheretemporaryprogramscanbewritten,run,
andoverwritteneasily.Program“0”isconsideredtobean“open”
program.(See Section C.1, Programming the Pipette).

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Pipette Overview
[Purge] ThePurgekeyentersapurgepromptanytimeduringapipetting
operationtoaborttheprogram.BypressingthePurgekey,then
pressingthetrigger,youcandispenseanyremainingliquidfrom
thepipettetipsandreturntothebeginningoftheprogram.
ThePurgekeyisalsousedtoenterthepurgestepintoa
program.
Note: If there is insufcient liquid for a nal dispense step, the
pipette emits a beep tone and automatically displays the purge
prompt.
Note: The RUN mode performs the operation that is displayed, such as ll, dispense,
mix, or purge. When “Run” appears in the display, you can activate the RUN mode by
pressing the trigger. At the end of a pipetting sequence, the pipette emits a triple beep
tone and returns to the beginning of the program. The pipette remains in RUN mode
until you program a new pipetting sequence.
B.3.2 Programming Keys
[Fill]
[Disp]
[Mix]
TheFill,Dispense,andMixkeysprogramthepipettingoperations.
Bypressingthellormixkey,youcanentertheprogramming
modeoftheelectronicpipette.The“Run”promptdisappearsfrom
thedisplayandtheappropriateoperationappearswithaashing
“Vol”prompt.Fill,dispense,ormixvolumescanbeprogrammed
bypressingtheVolumekey.Also,thenumberofmixcyclescan
beprogrammedbypressingtheMixkeytwice.
Automatic blowout:Attheendofanaldispensestep,the
pipetteautomaticallyexpelsanyremainingliquidfromthepipette
tipswithashortburstofair(blowout).Thepistonsthenreverse
directionandreturntozeroposition.

8
Pipette Overview
Note:Ifyoupressandholdinthetriggerduringthenal
dispensestep,thepipetteperformstheautomaticblowoutand
holdsitspositionuntilyoureleasethetrigger.Thisdelayallows
yousufcienttimetoremovethetipsfromtheliquidtoprevent
aspirationofliquidasthepistonsreturntozeroposition.
TheVolumekeycontrolsthequantityofliquidtobeaspiratedor
dispensed.Thevolumecanbeenteredwhenthe“Vol”prompt
appears.
Pipettingvolumecanbeincrementedquicklybycontinuously
depressingtheVolumekey.Thelongeryouholdthekey,the
greatertheincrementalspeed.
TheVolumekeycanalsobeusedtochangepipettingspeeds
withinastoredprogramorduringtheexecutionofaprogram.
(See Section C.1.5, Adjusting Pipetting Speed).
[Enter] TheEnterkeystorestheprograminmemory.Aftereachstepis
programmed,theEnterkeyispressedoncetoacceptthestep.
Afterthenalstepisprogrammed,theEnterkeyispressedtwice
todenotetheendoftheprogram.Thepipetteveriestheendof
theprogramwithatriplebeeptone.
B.3.3 Special Function Key
TheSpecialFunctionkeyisthesmallholebelowtheVolumekey.Itcanbe
activatedwithapipettetip.ByrepeatedlypressingtheSpecialFunctionkey,
youcandisplayfourfunctions:PipettingSpeed(SPd),BeepTone(TONE),
PacedDispenseSpeed(PACE),andCalibration(CAL).ToexittheSpecial
Functionkeyatanytime,presstheEnterkey.
Vol
t
s

9
Pipette Overview
SPd PipettingspeedcanbeadjustedbypressingtheVolumekeyin
thespecialfunctionmode.Fivespeedsettingsareavailable:
SpeedSetting Display
Slow S
MediumSlow Sz
Medium Sz z
MediumFast Sz z z
Fast Sz z z F
TONE
Thebeeptonesignalsthecompletionofoneormoresteps,or
signalsawarning:
• Single beep tone:endofpipettingstep.
• Double beep tone:operationorprogrammingkeyisnot
activeincurrentmode;insufcientdispensevolume,purge
required;orillegalprogrammingstepattempted(see Section
E, Troubleshooting).
• Triple beep tone:endofpipettingsequence;endof
programmingsequence.
Therearethreeoptionstochoosefrominsettingthebeeptone.
ON1soundsalltonesattheappropriatetimeslistedabove.ON2
usesonlydoubleandtriplebeeptones.OFFturnsoffalltones
exceptforthetonetosignifyanillegalprogrammingstep.Press
theSpecialFunctionkeytwicetodisplay“TONE”anditscurrent
setting.Tochangethesetting,presstheVolumekey.Ifthebeep
toneissetforON1orON2,thebeeptonesymbol“ ”appears
atthebottomofthedisplay.
Note:Forpaceddispensing,thebeeptoneshouldbesetON1,to
prepareyouforeachdispensestep.

10
Pipette Overview
PACE Thepacecontrolsthespeedofrepeatpipetting(paced
dispensing),FAST,SLOWorOFF.PresstheSpecialFunctionkey
threetimestodisplay“PACE”anditscurrentsetting.Tochange
thesetting,presstheVolumekey.
PacedDispense.Whenusingthepaceddispensefeature,the
pipettedispensestheprogrammedvolumeatthespeciedpace
(fast or slow).Immediatelyaftereachdispensestep,thepipette
emitsasinglebeeptone(if beep tone is on)toprepareyouforthe
nextdispensestep(see Section C.5, Operating Procedures).
CAL Thecalibrationofthepipetteisfactorysetfordistilledwaterat
roomtemperature.Ifyouarepipettinguidswithsignicantly
differentspecicgravitiesortemperatures,usethissettingto
recalibratethepipette.(See Section D, Calibration).
Thecurrentspeedsettingappearsatthebottomofthedisplay.Thesesettings
canbeselectedandchangedatanytimebeforeaprogramstepisexecuted.
B.4 Display
ThepipettedisplayisadynamicLCDscreen.Itdisplaysthecurrentoperation,
volumeprompt(if entering a program),pipettingvolume,beeptonesymbol(if
the beep tone is on),pipettingspeed,andprogramnumber.
Figure B.4: Pipette Display

11
Pipette Overview
B.5 Pipette Specications
Item
No. Volume Channel Volume Range
Color
Ring
Accuracy*
(±)
Precision**
(+)
1029 12.50µl Single Ch 0.50-12.50µl Red 1.0%/0.1ul 0.4%/0.06ul
1020 30.0µl Single Ch 1.0-30.0iµl Purple 1.0%/0.15µl 0.3%/0.05µl
1021 125.0µl Single Ch 2.0-125.0µl Yellow 0.6%/0.3µl 0.2%/0.1µl
1022 250µl Single Ch 5-250µl Blue 0.6%/0.5µl 0.15%/0.15µl
1024 1250µl Single Ch 15-1250µl Green 0.5%/3.0µl 0.13%/0.6µl
1122 250µl Shorty 5-250µl Blue 0.6%/0.5µl 0.15%/0.15µl
1124 1250µl Shorty 15-1250µl Green 0.5%/3.0µl 0.13%/0.6µl
2621 125.0µl 6-ChEXP 2.0-125.0µl Yellow 2.0%/1.0µl 1.0%/0.6µl
2622 250µl 6-ChEXP 5.0-250.0µl Blue 2.0%/1.5µl 0.7%/1.0µl
2624 1250µl 6-ChEXP 15-1250µl Green 1.5%/6.0µl 0.6%/3.0µl
2009 12.50µl 8-Ch 0.50-12.50µl Red 2.5%/0.15µl 2.0%/0.15µl
2029 12.50µl 8-ChEXP 0.50-12.50µl Red 2.5%/0.15µl 2.0%/0.15µl
2001 125.0µl 8-Ch 2.0-125.0µl Yellow 2.0%/1.0µl 1.0%/0.6µl
2021 125.0µl 8-ChEXP 2.0-125.0µl Yellow 2.0%/1.0µl 1.0%/0.6µl
2002 250.0µl 8-Ch 5.0-250.0µl Blue 2.0%/1.5µl 0.7%/1.0µl
2022 250.0µl 8-ChEXP 5-250µl Blue 2.0%/1.5µl 0.7%/1.0µl
2004 1250µl 8-Ch 15-1250µl Green 1.5%/6.0µl 0.6%/3.0µl
2024 1250µl 8-ChEXP 15-1250µl Green 1.5%/6.0µl 0.6%/3.0µl
2019 12.50µl 12-Ch 0.50-12.50µl Red 2.5%/0.15µl 2.0%/0.15µl
2229 12.50µl 12ChEXP 0.50-12.50µl Red 2.5%/0.15µl 2.0%/0.15µl
2011 125.0µl 12-Ch 2.0-125.0µl Yellow 2.0%/1.0µl 1.0%/0.6µl
2012 250.0µl 12-Ch 5.0-250.0µl Blue 2.0%/1.5µl 0.7%/1.0µl
2014 850µl 12-Ch 15-850µl Orange 1.5%/4.0µl 0.6%/2.5µl
2069 12.50µl 16-Ch 0.50-12.50µl Red 2.5%/0.15µl 2.0%/0.15µl
2060 30.0µl 16-Ch 1.0-30.0µl Purple 2.0%/0.30µl 1.7%/0.30µl
2061 125.0µl 16-Ch 2.0-125.0µl Yellow 2.0%/1.0µl 1.0%/0.6µl
Table B.5 Pipette Specications
Note:ForAccuracyandPrecision,valuesshownareexpressedasapercent
(%)deviationormicroliter(µl)value.Whenappliedtodesiredvolume,the
greaterofthetwovalueswillalwaysapply.
*Bothvaluesrepresentthedeviationfromthemean.
** Percentagevaluesareexpressedasthecoefcientofvariation.Microlitervaluesare
 expressedasthestandarddeviation.

12
Pipette Overview
B.6 Hazards and Precautions
TherearenoknownhazardsassociatedwiththeThermoScienticMatrix
ElectronicPipettewhenitisoperatedinaccordancewiththeinstructionsin
thismanual.However,youshouldbeawareofsituationsthatcanresultin
seriousinjury.
Note: Do not perform troubleshooting procedures on the internal components
unless instructed byThermo Fisher Scientic Technical Service personnel.
WARNING!Ensurethatthepowertransformerisconnectedtoa
powerreceptaclethatprovidesvoltageandcurrentspeciedby
ThermoFisherScientic.Useofanincompatiblepowerreceptacle
cancauseshockandrehazard.
CAUTION! UseonlythepowertransformersuppliedbyThermo
FisherScientic.Useofanincompatiblepowertransformercan
damagethepipette.
CAUTION!Alwaysturnoffthepowerandunplugthepower
transformerbeforecleaningtheexterior.Fluidseepagecan
damageinternalcomponents.
CAUTION!Donotimmersetheunitinliquid.Fluidseepagecan
damageinternalcomponents.
CAUTION!Donotautoclavetheentireunit.Extremeheatcan
damagethedisplayandotherelectroniccomponents.
Note: For autoclaving specic portions of the pipette, refer to the
Autoclaving Procedures, or call Thermo Fisher Technical Service
for instructions.
CAUTION!Donotcleanthekeypadwithbleachsolutionorother
solvents.Causticcleaningsolutionscandamagethekeypad.
CAUTION!Avoidexcessivechargingofthebatterieswhenthe
pipetteisnotinuse.Excessivechargingshortensbatterylifeand
maydamagethebatteries.

13
Programming and Operation
C. PROGRAMMING AND OPERATION
C.1 Programming the Electronic Pipette
ProgrammingtheMatrixelectronicpipetteisfastandeasy.Pipettingsteps
andvolumeareenteredinthesequencethattheywillbeperformed,thenthe
programissavedtomemory.Thisprogramwillstayinmemory(evenifthe
pipetteisturnedoff,ortheelectronicpipettebatteryrunslow)untilyouchange
it.Fiveprogramsandonescratchpad(Program0),eachcapableofholding
40steps,canbeenteredandstoredintheelectronicpipette.
C.1.1 Open Programming Mode or “Scratch Pad” (Program 0)
TheMatrixelectronicpipetteprovidesampleprogramstorageforve,
40-stepprograms.Inaddition,itoffersopenprogrammingmode,also
knownasthe“scratchpad.”Thismodeisidentiedby“P-0,”which
appearsintheprogramsectionofthedisplay(refer to the pipette
display on page 10).Thisfeatureenablesquickchangestopipetting
protocolswithoutaffectingpreviouslystoredprograms.
Tousethe“scratchpad”mode:Fromthestartofanyprogram,
simplybeginenteringanewprotocol(selecting a pipetting operation
as described below).Thisnewprotocolcannowbeused,andwill
remainactiveuntilastoredprogramisretrieved.“P-0”appearsin
theprogramIDsectionofthedisplaytoindicatethatthe(O)pen
programmingmode,or“scratchpad”mode,isactive.
C.1.2 Entering a Program in Open Programming Mode
Toenterapipettingprogram:
1. Turn on pipette. Pressthegreen[On/Off]keytoturnonthepipette.The
pipettewillbeeponceandash“ZERO”onthedisplaywhileresettingthe
pistonstozeroposition.Therststepofthecurrentprogramisdisplayed.
Note: If the pipette is being programmed for the rst time, the rst step is the ll
operation, with the default ll volume displayed (see the following table).

14
Programming and Operation
Volume Fill
Volume
Dispense
Volume
Mix
Volume
12.50 10.00 10.00 5.00
30.0 25.0 25.0 10.00
125.0 100.0 100.0 50.0
250.0 250.0 50.0 50.0
850 850 100 100
1250 1250 100 100
Table C.1.2: Default Programming Volumes (in µl)
2. Select pipetting operation. Entertheprogrammingmodebypressingthe
[Fill]or[Mix]key.
For example, to begin
programming with a lling
operation, press the [Fill] key.
The display shows “Fill” with
a ashing “Vol” prompt:
Note: The displayed volume defaults to the last volume programmed for the
selected operation, or to the default volume for the pipette, if it is being programmed
for the rst time.
Fill.The[Fill]keyprogramsthepipettortoaspirateavolumeofliquid.A
singlellstepcanbeprogrammedforuidtransfers,oracombinationofll
stepscanbeprogrammedfordilutionsorsupernatantcollection.
Dispense.The[Disp]keyprogramsthepipettetodispenseavolumeofliquid.
Thedispensevolumecaneitherbetheentirellvolumeorasmallerincrement
forrepeatpipetting.Thedispensevolumeneedstobeprogrammedonlyonce
forrepeatpipetting.Thepipettewilldispensethevolumeeachtimethetrigger
ispressed(orrepeatedlyduringapaceddispense)untilthellvolumeis
reached,oruntilinsufcientvolumeremains.
Figure C.1.2a: Flashing Volume Display

15
Programming and Operation
Mix.The[Mix]keyprogramsthepipettetoperformacombinationoflls
anddispenses.Youcanprogramamixvolumeormixcycle(singlelland
dispense).Youcanpress[Mix]oncetoprogramamixvolume,ortwiceto
programamixcycle.Mixcyclesfrom2to9areavailableonthepipette.
Thedefaultsettingisthreemixcycles.
Someprogrammingconsiderationsforthemixoperation:
•Iftheprogrambeginsamixcyclefromzeroposition,itwillcompletethe
cyclewithanautomaticblowout,thendisplay“ZERO.”Pressthetrigger
toreturnthepistonstozeroposition.
•Iftheprogrambeginsamixcycleabovezeroposition(volumeis
greaterthanzero),theuidisdispensed,andthemixcyclebegins.
Themixcyclewillendatthesamevolumeitstartedwith.Also,themix
operationwillremaininthedisplaysoitcanberepeated(usefulfor
serialdilutions),unlessfollowedbyanotherprogramstep.Toendthemix
operation,pressthe[Purge]key,thenpressthetrigger.
Purge.The[Purge]keyprogramsthepipettetopurgetheuid.Thisstepcan
beenteredasaprogramstep,oritcanbepressedanytimewhilepipettingto
terminatetheprogramanddispensetheremainingliquid.
Note: If the program ends at a volume greater than zero, and the last step is
not a mix cycle, the “Purge” prompt automatically appears. Also, the pipette
emits a double beep tone to warn you that some uid remains. Press the trigger
to dispense the remaining uid and return to the beginning of the program.
Examples of pipetting programs with different mix steps are shown in
Section C.2, Programming Examples.
3. Enter volume.Enterthedesiredvolumefor[Fill],[Disp],or[Mix]by
pressingtheupsordowntsectionofthe[Vol]key.Thelongeryouhold
thekey,thegreatertheincrementalspeed.
Note: The [Purge] key does not use a volume; therefore, if a purge step has
been entered, continue to the next step.