Thermo Scientific Matrix 1029 User manual

Thermo Scientific
Matrix Electronic Pipette
User Manual

Manual N09238
Thermo Scientific Matrix
Electronic Pipette
User Manual

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CONTENTS
Congratulations! ............................................................................................. 1
Before You Begin............................................................................................. 1
A. INTRODUCTION ....................................................................................... 2
 A.1ThermoScienticMatrixSingleChannelPipette ................................................3
 A.2ThermoScienticMatrixMultichannelPipette ....................................................3
B. PIPETTE OVERVIEW................................................................................. 4
 B.1Components........................................................................................................4
 B.2GeneralDescription ............................................................................................5
 B.3Keypad ................................................................................................................6
 B.3.1OperationKeys ................................................................................................6
 B.3.2ProgrammingKeys...........................................................................................7
 B.3.3SpecialFunctionKey .......................................................................................8
 B.4Display ..............................................................................................................10
 B.5PipetteSpecications........................................................................................11
 B.6HazardsandPrecautions..................................................................................12
C. PROGRAMMING AND OPERATION....................................................... 13
 C.1ProgrammingtheElectronicPipette .................................................................13
 C.1.1OpenProgrammingModeor“ScratchPad”(P-0) .........................................13
 C.1.2EnteringaPrograminOpenProgrammingMode..........................................13
 C.1.3StoringaProgram..........................................................................................16
 C.1.4ProgramRecall ..............................................................................................17
 C.1.5AdjustingPipettingSpeed ..............................................................................17
 C.1.6ReviewingtheProgram..................................................................................17
 C.2ProgrammingExamples....................................................................................18
 C.2.1SampleTransfers...........................................................................................18
 C.2.2IncrementalPipetting .....................................................................................19
C.2.3 Serial Dilutions ...............................................................................................19
 C.2.4SimpleDilutions .............................................................................................20
 C.2.5SupernatantCollection...................................................................................20
 C.3OperatingProcedures.......................................................................................21

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D. CALIBRATION ......................................................................................... 23
 D.1VolumeMeasurement .......................................................................................23
 D.2PipetteCalibration.............................................................................................24
 D.2.1FactoryCalibrationStep ................................................................................24
 D.2.2“EASY”CalMode...........................................................................................24
 D.2.3“CALC”Mode .................................................................................................25
 D.2.4CALVIEW .......................................................................................................27
 D.2.5CALRESET ....................................................................................................27
 D.3RecommendationsforAccuratePipetting.........................................................27
E. TROUBLESHOOTING.............................................................................. 29
F. MAINTENANCE ........................................................................................ 31
 F.1SingleChannel Maintenance.............................................................................32
 F.1.1SingleChannel Disassembly ..........................................................................32
 F.1.2SingleChannelCleaningandAutoclaving......................................................34
 F.1.3PipetteReassembly........................................................................................34
 F.2Multichannel Maintenance .................................................................................35
F.2.1Multichannel Disassembly ..............................................................................35
F.2.2MultichannelCleaningandAutoclaving ..........................................................38
 F.2.3MultichannelMaintenanceandReassembly ..................................................38
 F.3Installing/ReplacingBatteries ............................................................................44
 F.3.1Installing/ReplacingNiMHBatteries ...............................................................44
 F.3.2Installing/ReplacingLithium-IonBattery(16Ch) ............................................48
 F.4Charging/RechargingBatteries..........................................................................50
 F.4.1Charging/RechargingNiMHBatteries.............................................................50
 F.4.2Charging/RechargingLithium-IonBattery(16Ch)..........................................51
G. TECHNICAL SERVICE ............................................................................ 53
 G.1InstrumentWarranty .........................................................................................53
 G.2ExpertPipetteService ......................................................................................54
 G.3OnSitePipetteServiceOptions .......................................................................55
 G.4RecommendedEquipmentDecontaminationMethods ....................................56
 G.5TipsandReservoirs ..........................................................................................58

1
Congratulations!
Youhavepurchasedthestate-of-the-artinprecisionpipetting.TheThermo
ScienticMatrixElectronicPipetteistherstcordlesselectronicpipettethat
“understands”themyriadofliquidhandlingprotocolstypicallyperformedin
today’slaboratory.
Ergonomicallydesignedandbalancedtoreducefatigue,theMatrixelectronic
pipettefunctionssointuitively,youmayneedonlyaquicksurveyofthis
manual to use it.
Theelectronicpipetteexecutesyourliquidpipettingtasksquickly,accurately,
andwithlessriskofdevelopingrepetitivestraininjuries(RSI)typically
associatedwithmanualpipetteuse.Laboratoryresultswillbecomemore
standardized,whilelesstimeisexpendedontediousbenchprocedures.
Before You Begin
BeforeusingtheMatrixelectronicpipette,verifythecontentsofthepackage.
Thepackageshouldcontainthefollowingitems:
1ElectronicPipette
1Operator’sManual
2RechargeableNiMHBatteriesor1Lithium-IonBattery(16 Channels)
1ACPowerTransformer
1WarrantyCard
1Accuracy/PrecisionDataSheet
Ifitemsaremissing,contactaThermoFisherScienticrepresentativeor
contactcustomerserviceat 800-345-0206.
Note: Batteries must be installed and recharged before using the Matrix
electronic pipette Refer to the following sections in this manual:
Section F.3, Installing/Replacing Batteries
Section F.4, Charging/Recharging Batteries

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A. INTRODUCTION
TheThermoScienticMatrixElectronicPipetteprovidestheconvenienceof
amanualpipettor,withtheprogrammingcapabilitiesofanelectronicpipette.
TheMatrixelectronicpipettefeaturesinclude:
•Expandedmemoryforstoringuptovepipettingprograms.
•Eachprogramcanholdamaximumof40steps.
•“ScratchPad”programmingmodewhichallowsforfastandsimple
programchangesthatarenotstoredintomemory.
•Enhancedspeedcontrolofferssloweraspiratinganddispensingof
viscousliquids.Ideal for gel loading!
•“EASY”CALwhichsimpliesin-labcalibrationvericationusing
distilledwater.
TheMatrixelectronicpipetteislightweightandcordless,utilizingrechargeable
batteries,thatcanbeusedduringrecharge(with the exception of single
channel pipettes).Thepipettealsohasanautomaticshut-offfeaturetoextend
batterylife.
Theelectronicpipetteusesintegratedcircuittechnologytostoreprogramming
information.Itwillholdupto40stepsinaprogram,withupto5programs
savedinmemory,and1scratchpad(See section C.1.1 Open Programming
Mode or “Scratch Pad”).Theprograminformationisretainedinmemoryeven
ifthebatteriesarefullydischargedorremoved.
Theelectronicpipette usesa“paceddispense”featurethatperformsrepeat
pipettingstepswhilethetriggerisdepressed.

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Introduction
A.1 Thermo Scientic Matrix Single Channel Pipettes
Thesinglechannelelectronicpipetteareavailableinanumberofmodel
congurations(see Figures A-1)from12.5µlto1250µl,ranginginvolume
from0.5µlto1250µl.Eachpipetteiscolor-codedforeasyvolume
identication(see section B.5, Pipette Specications).Twomodels,250µland
1250µl,areofferedwithashorterejectorsleevereducingtheoveralllength
comparedtothestandardpipette.Idealforuseinconnedspacessuchas
biosafetycabinet
A.2 Thermo Scientic Matrix Multichannel Electronic Pipettes
Themultichannelelectronicpipettesareavailableinanumberof
congurationsfrom8to16channnels.AnexpandableEXPpipetteis
alsoavailablein6-channelandcustomcongurations.Modelsrangein
volumefrom0.5µlto1250µl.Eachpipetteiscolor-codedforeasyvolume
identication(see section B.5, Pipete Specications).TheThermoScientic
MatrixEqualizer®pipettehasequaltipspacingbetween4.5mmand14.15
mm(depending on model).
6-Channel
Expandable
8-Channel 12-Channel 16-Channel
384Model
1250µl 250µl 125µl 30µl 12.5µl
1250µl
Shorty
250µl
Shorty
Figure A.1: Single Channel Models
Figure A.2: Multichannel Models

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Pipette Overview
B. PIPETTE OVERVIEW
B.1 Components
Figure B.1b: Multichannel Components
Figure B.1a:
Single Channel Components

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Pipette Overview
B.2 General Description
Keypad.Thekeypadcontainstheoperationkeysthatareusedin
programmingthepipettingsequenceandvolumes.
(See Section B.3, Keypad).
Display.ThedisplayisanLCDscreenthatshowsthepipettingoperation
(ll, dispense, mix, or purge)anduidvolumeforeachstepinthepipetting
program.(See Section B.4, Display).
Battery Case.Thebatterycasecontainstherechargeablebatteries.Nickel
MetalHydridebatteriesfullychargein14-16hours,whileLithium-Ionbatteries
fullychargein2hours.Thepipettecanperformafullday’sworkloadwithno
needtorecharge.Batterylifeisextendedwithautomaticshut-offafter
10minutesofinactivity.(See Section F.3, Installing/Replacing Batteries).
Pipetting Trigger.Thepipettingtriggerinitiatesaprogram.Asinglepipetting
stepisexecutedbyaquickpressandreleaseofthetrigger.Repeatpipetting
isexecutedbypressingandholdingthetrigger.
Note: At the end of any dispense, the pipette tips should be removed from the
uid before releasing the trigger, to prevent aspiration of uid.
Ejector Trigger.Theejectortriggerispressedtoejectthetips
fromthepipette.
Tip Fittings.Thetipttingsformanairtightsealwiththetipstoensure
pipettingaccuracy.
Note: Electronic pipettes are equipped with chemically-resistant (PEEK) tip
ttings (and ejector sleeves on single channel models) that allow pipetting of
acidic solutions without damage to the tip ttings.
Color-coded Ring.Theringaroundthepipettehandleiscolorcoded
toidentifythepipettingvolumeofthehandle(See Section B.5, Pipette
Specications).
Transformer Connector.Thetransformerconnectorreceivestheplugfrom
thepowertransformerforrechargingthebatteries.Thepipettecanbeused
whilerecharging(with the exception of single channel pipettes).