Thermo Scientific MaxQ Mini 4450 series User manual

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MaxQ Mini 4450 Shaker
Model SHKA4450 and SHKE4450
Operating Manual and Parts List 057-810-00 (7004324) Rev. 14
User Manual

Thermo Scientific
MANUAL NUMBER 057-810-00 (7004324)
14 40722 7/10/17 Added gas sprigs statement to Maitenance bpg
13 40453 9/13/16 Updated circuit breaker P/N in Replacement Parts from 330-399-000 to 330-138-00 ccs
12 40139 4/15/15 Updated warranty information ccs
11 31361 7/18/14 Removed CE reference from pg 2-1 ccs
10 31164/OS-775 7/9/14 Added care and cleaning of the acrylic lid to Section 7 ccs
9 30954 4/10/14 Updated Setting Timer for Continuous Shaking Step 3 - pg 5-8 ccs
8 30837/OS-738 2/27/14 Removed obsolete Model 4326 ccs
7 29034 9/10/12 Updated hyper terminal info on pg 5-9
Preface
MaxQ Mini 4450 i
Models covered by this manual
Model number Voltage
SHKA4450 (4324) 120V
SHKE4450 (4333) 120V
SHKA4450-1CE (4325) 240V
SHKE4450-1CE (4334) 240V
SHKA4450CC (4340) 120V
SHKE4450CC (4345) 120V
SHKA4450CC-1CE (4341) 240V
SHKE4450CC-1CE (4367) 240V
SHKE4450GM (4335) 240V
SHKE4450CCGM (4347) 120V

Thermo Scientificii MaxQ Mini 4450
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. n no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
©2010 Thermo Fisher Scientific. All rights reserved.

Thermo Scientific MaxQ Mini 4450 iii
Preface
mportant operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal in
each EU Member State. For further information, send an email to [email protected].
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv MaxQ Mini 4450
Preface
Do You Need Information or Assistance on
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Regardless of your needs, our professional telephone technicians are available to assist you
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International customers, please contact your local Thermo Scientific distributor.

MaxQ Mini 4450 vThermo Scientific
Table of Contents
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Product Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Analog Control Panel Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Digital Control Panel Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
General Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
Environmental Operating Conditions . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Unpacking and Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Platform nstallation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Test Tube Rack nstallation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Flask Clamp nstallation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
peration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Analog . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Timer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Speed Control and Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Power Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Temperature Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Temperature Controller/ Setting Temperature . . . . . . . . . . . . . . . . 5-2
Turning Shaker On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Section 1
Section 2
Section 3
Section 4
Section 5

vi MaxQ Mini 4450 Thermo Scientific
peration (continued)
Digital . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Setting High-Limit Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Calibrating Shaking Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Setting Shaking Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Setting High-Limit Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Setting Operating Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Temperature Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-7
AC Power Loss . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
Setting Timer for Continuous Timing . . . . . . . . . . . . . . . . . . . . . . 5-8
Setting Timer for Continuous Shaking . . . . . . . . . . . . . . . . . . . . . . 5-8
Setting Timer for Timed Shaking . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
RS232 Communication Configuration . . . . . . . . . . . . . . . . . . . . . . . 5-9
RS232 nterface Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-9
Using the Optional Cooling Coil . . . . . . . . . . . . . . . . . . . . . . . . . . .5-10
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Over Temperature Protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-2
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
Gas Springs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
Replacing Platform Mounting Plate . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Platform Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-2
Care and Cleaning of the Acrylic Lid . . . . . . . . . . . . . . . . . . . . . . . . .7-2
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1
Ordering Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-3
Table of Contents
Section 6
Section 5
Section 8
Section 7

MaxQ Mini 4450 1-1Thermo Scientific
Section 1 Safety Information
Your Thermo Scientific MaxQ Mini 4450 Shakers have been designed
with function, reliability, and safety in mind. t is your responsibility to
install it in conformance with local electrical codes. For safe operation, pay
attention to the cautions and warnings throughout the manual.
This manual contains important operating and safety information. The
user must carefully read and understand the contents of this manual prior
to the use of this equipment.
Warnings
• Do not modify construction and/or assembly of equipment.
• Do not remove tags, labels, decals or other information from the unit.
• Stand clear of equipment when it is operating.
• f shaking action will result in the evolution of gases or fumes, carry
out the operation in a well-ventilated laboratory hood.
• Do not use equipment for other than its intended purpose. Use only
the accessories and attachments that are shipped with the equipment
or are specified for it. Substituting other attachments or accessories can
produce hazards or make the unit inoperative.
• Perform regular maintenance service as specified in this manual and
keep unit in good repair. Do not operate with known defects.
• Do not use the shaker to mix flammable materials or where the
transfer of mechanical energy to glass could cause glass breakage.

MaxQ Mini 4450 2-1Thermo Scientific
Section 2 Product Profile
The Thermo Scientific MaxQ Mini 4450 bench top, incubated shakers are
available in either analog or digital control configurations:
• Analog shakers: SHKA4450, SHKA4450CC, SHKA44500-1CE and
SHKA4450CC-1CE: control temperature by a Proportional/ ntegral/
Derivative microprocessor-based controller. Solid-state control
maintains time and speed and is adjustable with rotary dials. Analog
tachometer displays speed in RPM, verifying accuracy of speed setting.
• Digital shakers: SHKE4450, SHKE4450CC, SHKE4450-1CE and
SHKE4450CC-1CE: control temperature, time and speed by a
Proportional/ ntegral/Derivative (P D) microprocessor-based controller
that is adjustable with membrane switches on a keypad in 1 RPM
increments. Flashing display indicates power interruption. Pressing any
key will clear display. Non-volatile memory maintains speed and time
set points in the event of a power interruption. Speed and time set
points are automatically reactivated after power is restored.
• Models SHKA4450CC, SHKA4450CC-1CE, SHKE4450CC and
SHKE4450CC-1CE include a cooling coil to allow a below ambient
temperature in the shaker's chamber.
• Temperature range 5ºC above ambient to 80ºC.
• Drive interrupt halts shaking action when lid is opened.
• All set points are retained by non-volatile memory that automatically
reactivates after power is restored.
• Visual, user adjustable over-temperature safety signal with independent
thermostat controls the heat if main controller fails.
• 3/4 inch (1.9 cm) triple eccentric orbital drive.
• 6 permanently lubricated ball bearings.
• 35 lb (15.9 kg) platform load capacity at safe speeds less than 400 rpm
for analog shakers and less than 500 rpm for digital shakers.

2-2 MaxQ Mini 4450 Thermo Scientific
Section 2
Product Profile
Analog Control Panel
Features
1. SPEED Control: Sets platform rotation speed.
2. H GH-L M T Light: lluminates when high limit thermostat is
controlling temperature.
3. TEMPERATURE Controller: Maintains temperature.
4. T ME(R): Allows user to choose continuous timing or set timed
operation.
5. Heat Switch: Activates heater, turns heat on and off.
6. Speed Tachometer: Analog display of platform rotation speed (RPM).
12 3 4 5 6
Figure 2-1. Analog Control Panel
1
2
34
5
6-8 9
10 11-12 13
14
15-18
19-20
21 22
23-24
25
26
Figure 2-2. Digital Control Panel

MaxQ Mini 4450 2-3Thermo Scientific
Section 2
Product Profile
Digital Control Panel
Features
1. SPEED Display: 3-digit LED indicates actual or set point speed
2. Up Arrow Switch: ncreases platform rotation speed
3. Down Arrow Switch: Decreases platform rotation speed
4. STOP Switch: Stops platform rotation
5. START Switch: Starts platform rotation
6. SPEED Light: Red light lluminates when a locked rotor or over-speed
condition exists
7. RPM Light: lluminates to indicate actual speed
8. SET RPM Light: lluminates when speed is being set
9. POWER Switch: Turns power on and off to shaker
10. T ME Display: 3-digit LED indicates time remaining or elapsed time
11. Up Arrow Switch: ncreases shaking time
12. Down Arrow Switch: Decreases shaking time
13. T MER/ELAPSED: Allows the user to choose elapsed time operation,
ELAPSED, or timed operation, T MER
14. HOURS/M NUTES Membrane Switch: Allows user to choose timing
operation in either hours or minutes
15. T MER: Allows the user to choose elapsed time operation, ELAPSED
is the default setting. User must press T MER switch for timing
operations
16. HOURS: Timer indicates hours
17. M NUTES: Timer indicates minutes
18. SET T ME: lluminates when time is being set
19. Up Arrow Key: ncreases temperature
20. Down Arrow Key: Decreases temperature
21. HEAT ON Switch: Turns on heat
22. HEAT OFF Switch: Turns off heat
23. HEAT ON Light: ndicates heaters are energized
24. SET TEMP Light: ndicates temperature can be set
25. TEMPERATURE Display: 3-digit LED indicates chamber
temperature
26. H GH L M T Light: ndicates hi-limit control has been activated.

MaxQ Mini 4450 3-1Thermo Scientific
Section 3 General Specifications
Catalog Number SHKA4450,
SHKA4450CC
SHKA4450-1CE,
SHKA4450CC-1CE
SHKE4450,
SHKE4450CC
SHKE4450-1CE,
SHKE4450CC-1CE
Electrical
Voltage AC 120 220-240 120 220-240
Amperage 4.5 2.5 4.5 2.5
Wattage 500 500 500 500
Frequency (Hz) 50/60 50/60 50/60 50/60
Speed Accuracy 40 to 400 RPM, ±10 40 to 400 RPM, ±10 15 to 500 RPM, ±1 15 to 500 RPM, ±1
Timer
Continuous or timed
operation from 1 minute
to 60 minutes
Continuous or timed
operation from 1 minute
to 60 minutes
Continuous or timed operation
from 0.1 hour up to 999 hours
or 0.1 minute to 999 minutes
Continuous or timed operation
from 0.1 hour up to 999 hours
or 0.1 minute to 999 minutes
Display
LED display indicates
temperature in 0.1ºC
increments. Analog
tachometer displays
speed in rpm
LED display indicates
temperature in 0.1ºC
increments. Analog
tachometer displays
speed in rpm
3 individual LED displays indi-
cate temperature, time and
speed simultaneously. 3 char-
acters, height 1/2 inches (1.27
cm)
3 individual LED displays indi-
cate temperature, time and
speed simultaneously. 3 char-
acters, height 1/2 inches (1.27
cm)
Mutable Alarms None None
Audible portion of the alarm
can be silenced for a period of
1 hour without deactivating
the actual alarm condition by
depressing any key.
Audible portion of the alarm
can be silenced for a period of
1 hour without deactivating
the actual alarm condition by
depressing any key.

3-2 MaxQ Mini 4450 Thermo Scientific
Section 3
General Specifications
Catalog Number SHKA4450,
SHKA4450CC
SHKA4450-1CE,
SHKA4450CC-1CE
SHKE4450,
SHKE4450CC
SHKE4450-1CE,
SHKE4450CC-1CE
Alarm Speed None None
Audible with flashing led
indicate when speed devi-
ates more than 10% of set
point
Audible with flashing led
indicate when speed devi-
ates more than 10% of set
point
Speed Shut Off None None
Timer
Continuous operation (hold)
or timed operation from 1 to
60 minutes.
Continuous operation (hold)
or timed operation from 1 to
60 minutes.
Beeps twice when time has
expired. Shaking motion
stops.
Beeps twice when time has
expired. Shaking motion
stops.
Unbalanced Load None None
f the unit is running in an
unbalanced condition, an
alarm will sound and the
shaker will stop until the end
user corrects the condition.
The speed display will flash
"bAL" on speed panel LED.
f the unit is running in an
unbalanced condition, an
alarm will sound and the
shaker will stop until the end
user corrects the condition.
The speed display will flash
"bAL" on speed panel LED.
Motor Permanent Magnet DC Permanent Magnet DC Solid State Brushless DC Solid State Brushless DC
Soft Start Feature None None Software algorithms prevent
sudden start/stops.
Software algorithms prevent
sudden start/stops.
Intended Use
Orbital shakers are designed to provide increased aeration in a stable
temperature environment.
Unintended Use
1) Not intended for use in Class or applications as defined in 21 CFR
2) Not intended for mixtures of flammable materials

MaxQ Mini 4450 3-3Thermo Scientific
Section 3
Specifications
Pollution Degree 2**
nstallation Category **
Altitude . . . . . . 2000 meters MSL (Mean Sea Level)
Relative Humidity . . 20% to 80% maximum, non-condensing
Electrical Supply . . . . . . . . . . 120 VAC or 240 VAC
Voltage Tolerance . . . . . . ±10% of normal rated line
Temperature . . . . . . . . . . . . . . . . . . . . 15°C to 32°C
Product Usage . . . . . . . . . . . . . . . . . ndoor use only
*Interface cables not to e ceed 9.8' (3 m) in length.
**Refer to IEC 664-1
Caution Operation of a shaker in a CO2 enriched atmosphere is not
recommended. The formation of carbonic acid could cause electrical
failures. s
Declaration of Conformity available on request.
Environmental
perating Conditions
Catalog Number SHKA4450,
SHKA4450CC
SHKA4450-1CE,
SHKA4450CC-1CE
SHKE4450,
SHKE4450CC
SHKE4450-1CE,
SHKE4450CC-1CE
RS232 nterface* None None
Monitor speed, tempera-
ture and time with a com-
puter
Monitor speed, tempera-
ture and time with a com-
puter
Recorder Output*
(Located on left side rear
of shaker)
None None
10mv/C output monitors
temperature with external
chart recorder
10mv/C output monitors
temperature with external
chart recorder
Optional Platform
Dimensions L X W 13" x 11" (33.0 x 27.9 cm) 13" x 11" (33.0 x 27.9 cm) 13" x 11" (33.0 x 27.9 cm) 13" x 11" (33.0 x 27.9 cm)
Exterior Dimensions
L x W x H
27.16" x 14.09" x 15.75"
(69.0 x 35.8 x 40.0 cm)
27.16" x 14.09" x 15.75"
(69.0 x 35.8 x 40.0 cm)
27.16" x 14.09" x 15.75"
(69.0 x 35.8 x 40.0 cm)
27.16" x 14.09" x 15.75"
(69.0 x 35.8 x 40.0 cm)

MaxQ Mini 4450 4-1Thermo Scientific
Section 4 Unpacking and Installation
The shipping carton should be inspected upon delivery. When received,
carefully examine for any shipping damage before unpacking. f damage is
discovered, the delivering carrier should specify and sign for the damage on
you copy of the delivery receipt.
Open the carton carefully making certain that all parts are accounted for
before packaging materials are discarded. After unpacking, if damage is
found, promptly report it to the carrier and request a damage inspection.
Important Failure to request an inspection of damage within a few days
after receipt of shipment absolves the carrier from any liability for damage.
Call for a damage inspection promptly.
Use the packing list below when unpacking to verify that the complete
unit has been received. Do not discard packing materials until all is
accounted for.
The following items are included in the shipment:
• nspection tag
• Mounting plate mat
• Thumbscrew knob
• Male connector (digital only)
f any items are missing, contact Technical Services.
• Put the shaker on a level table or bench capable of supporting the
weight of the shaker with any accessories while in operation.
• Place shaker near an electrical outlet that matches the unit's nameplate
requirements.
• Allow approximately 2" (5 cm) of clearance around the unit for free air
convection, accessory attachments and user convenience.
Unpacking
Location

4-2 MaxQ Mini 4450 Thermo Scientific
Section 4
Unpacking and nstallation
Electrical
Requirements
• SHKA4450, SHKA4450CC, SHKE4450 and SHKE4450CC shakers
require a 120VAC, 60Hz power source. They are supplied with a 3-wire
line cord and should be plugged into an outlet designed for 3-prong plugs.
f an extension cord is used, it also should be the 3-wire grounded type.
For an outlet designed to accept 2-prong plugs (ungrounded), it is required
that a qualified electrician replaces the outlet with a new, grounded type.
• SHKA4450-1CE, SHKA4450CC-1CE, SHKE4450-1CE and
SHKE4450CC-1CE shakers require a 240VAC, 50/60Hz power
source. Power cords are supplied.
• f a plug must be installed, use only the 3-prong grounded type, rated
for the unit load requirements and matching the power outlet. Make
sure the green ground wire is secured to the plug ground terminal.
• To eliminate hazard of electrical shock, make sure floor around shaker
is dry. n the event of accidental spilling or splashing of liquids, clean
up and/or neutralize the spilled liquids before continuing.
• Leave shaker disconnected when not in use.
Warning Do not operate shaker with a damaged electrical cord. s
1. Select the appropriate platform for the vessels to be shaken. A wide
variety of platforms and accessories are available:
• Dedicated platforms have the maximum number of flask clamps
attached for safe operation.
• Combination platforms allow the user to shake a wide variety of
different sized vessels on the same platform.
2. Carefully position the platform horizontally over the shaker's mounting
plate aligning the 4 mounting holes.
3. Positions one of the thumbscrews provided through each of the 4-
platform mounting holes and tighten securely.
Caution Do not operate shaker with an unbalanced load. Platforms should
be loaded for optimum stability. Do not lift shaker by the platform. s
Platform Installation

MaxQ Mini 4450 4-3Thermo Scientific
Section 4
Unpacking and nstallation
• Each flask clamp contains a support spring located at the narrow top
of the clamp.
• Depending on the size of the clamp, the clamp base may contain one
or several screws necessary to secure the clamp to the platform. All
screws provided with the clamp must be properly attached to the
platform.
1. Carefully place the desired vessel in the clamp by first pulling the
clamp spring far enough apart to enable the flask base to be positioned
inside the clamp. Secondly, gently slide the flask into its proper
position securing it to the wider bottom of the clamp. The spring will
hold the body of the flask securely in place and provide security during
shaking.
2. Make sure all vessels are securely clamped before turning on unit.
Wherever possible, vessels should contain a stopper to prevent
hazardous substances being thrown out during the mixing action.
Warning Do not operate the shaker at speeds that will cause the contents
of vessels to be thrown out. s
1. Position the test tube rack on the combination platform so that the
cutouts on the rack's outside bottom are aligned with corresponding
mounting holes on the platform. There are two cutouts on each side of
the rack.
2. Secure the rack to the platform with mounting screws provided with
the rack.
Flask Clamp
Installation
Test Tube Rack
Installation

MaxQ Mini 4450 5-1Thermo Scientific
Section 5 peration
Warning t is recommended that shaking action be started at a low speed
in order to check that all vessels are secure and that no spilling of contents
will occur. s
Note The shaker will not operate if the timer is in the Off position.
Refer to Section2 for control panel references.
1. Depress top portion of power switch to turn on shaker.
2. Depress bottom portion of power switch to turn off shaker.
1. Slowly rotate the knob on the solid-state speed control clockwise to
increase speed and counterclockwise to decrease speed. The markings
on the outside of the dial are for reference purposes only.
2. The speed control tachometer provides an analog readout of the actual
platform rotation speed up to a maximum of 400 rpm.
1. From the 12 o’clock off position, rotate timer knob counterclockwise
to the ON position to initiate continuous operation.
2. For timed operation, rotate timer knob clockwise from 1 minute to 60
minutes. The markings on the side of the dial are in 5-minute
increments.
Analog
Power Switch
Speed Control and Display
Timer

5-2 MaxQ Mini 4450 Thermo Scientific
Section 5
Operation
Refer to Figure 5-1 for control panel references.
1. CONTROLLER SELF-TEST: When the shaker is powered up, the
controller will display 8888 along with the three decimal points and
the HEAT ON indicator lamp. The display will then blank out for 2
seconds before showing the chamber temperature.
2. HEAT ON ND CATOR: The HEAT ON indicator lamp is lit when
the chamber heaters are receiving power. The lamp will normally flash
when the chamber temperature is at set point.
3. SET PO NT ADJUSTMENTS: The temperature controller normally
displays the chamber temperature. To view or change the temperature
set point proceed as follows:
a. Press and hold the “star” (*) key, and use either the up or down
arrow key to adjust the set point to the desired temperature.
Release the “star” (*) key.
b. Allow at least 30 minutes for the temperature to stabilize.
1. Fill a 250ml Erlenmeyer flask with approximately 100ml of water and
position it at the approximate geometric center of the shaking
platform.
2. nstall a thermocouple inside the flask with the thermocouple junction
in direct contact with the water.
3. Adjust the safety thermostat to its maximum clockwise position.
4. Press and hold the “star” (*) key and using the up or down arrow key,
adjust the set point to the desired temperature.
5. Allow the unit to run for at least 30 minutes.
Temperature Controller/
Setting Temperature
Temperature Calibration
Chamber or temperature
controller
Heat indicator
Control
buttons *s
s
Figure 5-1. Temperature Controller

6. The controller display should now be indicating the set point
temperature. Make note of the thermometer reading.
7. Press and hold both arrow keys until the controller display indicates
“tunE”. Release the arrow keys. Press and release the down arrow key,
the display should now alternate between “LEUL” and “1”. Press and
hold the “star” (*) key and using the up arrow key adjust the display to
read “3”. Release the “star” (*) key. The display should now alternate
between “LEUL” and “3”. Press and release the up arrow key until the
display indicates “Zero”. The display should now alternate between
“Zero” and a numerical value.
8. Using the examples shown in Table 5-1 and the thermometer value
obtained in Step 5 above, enter the correct “Zero” value into the
controller by pressing the “star” (*) key and using the up or down
arrow key. f there is already a “Zero” value present, then add the new
value to the one already present.
MaxQ Mini 4450 5-3Thermo Scientific
Section 5
Operation
Temperature Calibration
(continued)
Table 5-1. Determining Zero Value
Thermometer = 60°C
Controller Reading = 65°C
Subtract = -5°C
Enter Zero value of -5 °C
Thermometer = 70°C
Controller Reading = 65°C
Subtract = +5°C
Enter Zero value of +5 °C
9. When the correct “Zero” value has been entered, press and hold the
two arrow keys together until the display again indicates the chamber
temperature. f the procedure was done correctly, the controller display
should now agree with the thermometer reading to within ±0.5°C.
10. Allow the unit to run for at least an additional 60 minutes.
11. Re-check the thermometer reading. The controller display and the
thermometer should agree to within ±0.5°C. f not, repeat Steps 7, 8
and 9.