Thermo Scientific Sorvall WX+ User manual

Instruction Manual
50145792-a • 09 / 2014
Thermo Scientific
Sorvall WX+ Ultracentrifuge

General description
The Thermo Scientific™ Sorvall™ WX+ Ultracentrifuge is designed to separate liquid-suspended materials
having different densities and particle size.
The series is a series of products that pursue user-friendliness and reliability based on our many
years of experience in developing centrifuges. This series offers many new features that we are
confident of satisfying your requirements. These features include the following.
1. Maximum speed is 100,000 rpm (803,000 x g). (WX100+)
2. The touchscreen with easy-to-see color liquid crystal screen is incorporated.
3. The displayed language can be switched over between English and Japanese.
4. Color touch-sensitive liquid crystal display and graphic user interface with high contrast against
back screen in black color enable you to easily operate the system or select various menus
and functions by touching the icon on the display.
5. The real-time control feature enables setting a start time or finish time, thus letting you run your
machine at desired date and time.
6. Centrifugal force (RCFmax and RCFavg) can be displayed and set (Note1).
7. Thousand varieties of 30 stepped mode can be programmed for a wide range of applications
such as step running.
8. Various alert indication can notify users of the causes and necessary actions of the troubles.It
can realize troubleshooting easier and quicker.
9. The current centrifuge status is identified at a glance by the signal light of the upper front
edge of this centrifuge.
10. Space saving design. The installation area required is 0.72 m2 (800 x 900 mm). Lower top deck
makes it easy to install and remove the rotor.
11. These products spin very quietly, and are thus well suited for personal use.
12. Samples can be easily balanced visually.
13. A CFC-free thermomodule cooling system is employed featuring a powerful cooling capacity.
14. In addition to door lock and an imbalance detector, dual overspeed detection system is
incorporated for overspeed detection for even greater safety.
Note1: RCF = Relative Centrifugal Force
4

SAFETY NOTICES
Safety reminders
Carefully read and fully understand the following safety instructions.
●Operate your instrument according to the instruction manual.
●Be sure to observe the all safety precautions in the instruction manual and safety
instructions on your instrument. If neglected, personal injury and/or instrument damage
can be caused.
●If the equipment is used in a manner not specified by the manufacturer, the protection
provided by the equipment may be impaired.
●The safety reminders are indicated as shown below. The signal words "DANGER",
"WARNING" and "CAUTION" are indicated together with the hazard alert symbols in this
manual.
"NOTE" indicates a note which has no direct bearing on personal safety.
Do not perform any operation not specified in the instruction manual. If any problem is
found on your instrument, contact a Thermo Fisher Scientific authorized sales/service
representative.
Although the safety precautions in the instruction manual and safety instructions on your
instrument have been fully considered, an unexpected situation may arise. Observe the
instructions in the instruction manual and always be careful yourself when operating this
instrument.
DANGER：This note indicates an imminently hazardous situation, which if not
strictly observed, could result in personal severe injury or possible
death.
WARNING：This note indicates a potentially hazardous situation, which if not
strictly observed, could result i
n personal severe injury or possible
death.
CAUTION：This note indicates a potentially hazardous situation, which if not
strictly observed, could result in personal injury or severe damage
to the instrument.
This hazard alert symbol indicated together wit
h a signal word is a reminder
to emphasize important safety instructions.
i

SAFETY NOTICES
○Mechanical Safety
●Do not open the door while the rotor is spinning.
●Do not attempt to slow or stop the spinning rotor by hand.
●Do not incline or move the instrument while the rotor is spinning. Do not
place any object on the instrument or lean on the instrument.
●Do not attempt to unlock the door forcefully while the rotor is spinning.
●The centrifuge itself may move if the rotor fails during high-speed rotation.
Make sure there is a 30 cm area around the centrifuge that will allow for such
movement and do not allow individuals to enter into that area during
operation. Also do not place dangerous objects such as flammable or
explosive materials on top of the centrifuge or in the surrounding area.
●The overspeed decal must match the maximum speed of the rotor, because
the overspeed decal is a critical component that detects the over-speed of
the rotor: If an overspeed decal that is not compatible with the rotor is
attached, the rotor could break, resulting in damage to the ultracentrifuge.
●
Repairs, disassembly, and other modifications to the centrifuge are strictly prohibited
unless performed by a Thermo Fisher Scientific authorized sales/service representative.
●Do not use the other’s manufacturer’s rotor without Thermo Fisher Scientific's
permission.
●Check the chemical resistance chart attached to the rotor, and do not use
any sample inapplicable to the rotor (including the buckets). Using such a
sample could corrode the rotor (including the buckets).
●
Do not exceed the allowable maximum rated speed of the rotor or buckets in use.
●Do not use corroded, scratched or cracked rotor, buckets and assemblies.
Check that the rotor, buckets and assemblies are free of such abnormalities
before operation.
●When using a swing rotor, check that the buckets are properly engaged with
the rotor pins before operation. Wrong setting can cause severe damage to
the instrument. Make sure that all the rotor buckets are of the same type.
●If abnormal sound or vibration occurs, stop the operation immediately and
contact a Thermo Fisher Scientific authorized sales/service representative.
●Before using a rotor, be sure to read through the rotor instruction manual.
●Check the chemical resistance chart attached to the rotor, and do not use any
sample inapplicable to the tubes, tube caps, bottles, or bottle caps, etc.
Using such a sample could corrode or deteriorate such parts and the sample
leakage might occur.
●Use the rotor tubes and bottles within their actual capacities (see the
instruction manual).
●Do not use tubes/bottles that have exceeded their life expectancy. Failure to
do so could result in damage of tubes/bottles and the rotor and the centrifuge.
The life expectancy of tubes/bottles depends on factors such as the
characteristics of samples, speed of the rotor used, and temperature.
Always check for deterioration and damage (cracks, deformation, and so on) on
tubes/bottles before using them. Do not use the tubes/ bottles if you find such a problem.
●Mount the rotor onto the drive shaft gently and properly.
●Do not drop the rotor or apply excessive force to the drive shaft to avoid
damage to the drive shaft.
WARNING：
CAUTION：
ii

SAFETY NOTICES
●Install the rotor carefully and securely on the drive shaft (crown) in the rotor
chamber. Always place the rotor pin in the drive hole (crown hole) apart from
the crown pin.
●Take care not to get your hands or fingers caught in the door of the
centrifuge.
●Maximum rotor speed depends on the tubes or adapters to be used. Follow
the instructions on the rotor instruction manual.
●Approximately even quantities of sample in the tubes are sufficient for
balancing, and extremely different sample quantities must be avoided.
●Clean the inside of the drive hole (crown hole) of the rotor and the surface of
the drive shaft (crown) of the centrifuge once a month.
●Storing the rotor on the shelf is permitted if the shelf is taken necessary
countermeasures against earthquakes not to drop the rotor.
●Do not pour any solution such as water, detergent, or disinfectant directly into the
rotor chamber. Be careful not to leak the sample. Failure to do so might cause
the bearings of the drive unit and/or the sensors to corrode or deteriorate.
●Use the rotor log book to manage the life of the rotor.
●It is important to manage the life of the rotor. The life of each rotor is specific
and dependent upon the frequency and the total running time. Do not use
rotors whose lives have expired. If used, the machine can be seriously
damaged. (Follow the rotor instruction manual.)
●For details of the Zonal centrifugation, see the Zonal Rotor instruction manual.
●Do not press the touchscreen with a sharp-pointed object such as a ballpoint
pen.
●Be sure to remove the rotor from the rotor chamber when centrifuge is not
used for a long time or when the machine is moved. Otherwise the drive shaft
(crown) may be damaged.
○Safety during installation and maintenance
●To avoid electrical shock hazards, follow below when servicing the
centrifuge.
1) Be sure to turn off the POWER switch and turn off the distribution board of
your centrifuge room if your centrifuge is equipped with a three wire
power cord. Then wait at least three minutes and then remove the covers
from the centrifuge.
2) Be sure to turn off the POWER switch and unplug the power cord from the
receptacle if your centrifuge is equipped with a power cord with a plug.
Then wait at least three minutes and then remove the covers from the
centrifuge.
●When a power failure occurs during operation, it takes 3 hours or more for
the running rotor to stop completely because the rotor chamber is
depressurized and has less air to stop the rotor. Be sure to leave sufficient
time before opening the door of the rotor chamber.
●For maintenance and repairing of the rotors, tubes, etc., see the rotor
instruction manual and the rotor, tube, bottle, and cap instruction manual.
●After installation and before any test-run, this ultracentrifuge always needs
the internal check by a Thermo Fisher Scientific authorized sales/service
representative.
●Repairs, disassembly, and other modifications to the centrifuge are strictly
prohibited unless performed by a Thermo Fisher Scientific authorized
sales/service representative.
WARNING
：
CAUTION
：
DANGER
：
iii

SAFETY NOTICES
●If the centrifuge is exposed to ultraviolet rays for a long time, the color of the
covers may be changed or the coating may be peeled off. After use, cover
the centrifuge with a cloth to protect it from direct exposure.
○Electrical Safety
●Your centrifuge must be grounded properly to avoid electrical shock hazards.
●Do not place containers holding liquid in the rotor chamber or on or near the
instrument. If they spill, liquid may get into the instrument and damage
electrical components.
●If the machine will not be used for a long time, turn off the main circuit
breaker.
○Safety against Risk of Fire
●This centrifuge is not explosion proof centrifuge. Never use explosive or
flammable samples or materials that chemically react vigorously. Do not
centrifuge such materials in this instrument nor handle or store them near the
instrument.
○Chemical and Biological Safety
●Take all necessary safety measures before using samples that are toxic or
radioactive, or blood samples that are pathogenic or infectious. You use such
samples at your own responsibility.
●Take all necessary safety measures when Risk Group Ⅱ materials (as
identified in the World Health Organization “Laboratory Biosafety Manual”)
are handled, and that more than one level of protection shall be provided in
the case of materials of a higher group.
●If the centrifuge, rotor, or an accessory is contaminated by samples that are
toxic or radioactive, or blood samples that are pathogenic or infectious, be
sure to decontaminate the item according to good laboratory procedures and
methods.
●If there is a possibility that the centrifuge, rotor, or an accessory is
contaminated by samples that might impair human health (for example,
samples that are toxic or radioactive, or blood samples that are pathogenic or
infectious), it is your responsibility to sterilize or decontaminate the centrifuge,
rotor, or the accessory properly before requesting repairs from a Thermo
Fisher Scientific authorized sales/service representative.
●It is your responsibility to sterilize and/or decontaminate the centrifuge, rotor,
or parts properly before returning them to a Thermo Fisher Scientific
authorized sales/service representative.
Notice for an Earthquake
Depending on the magnitude, an earthquake might damage centrifuge. If you observe some
abnormality, stop using the centrifuge immediately and ask for inspection by the Thermo Fisher
Scientific service representative.
WARNING
：
WARNING
：
CAUTION
：
CAUTION
：
WARNING
：
iv

SAFETY NOTICES
Precaution indications in this Manual
The followings informations indicate the precaution indications and the chapters/sections
which mention them in this manual.
1.Indication of DANGER
To avoid electrical shock hazards, follow below when servicing the centrifuge.
1) Be sure to turn off the POWER switch and turn off the distribution board of your
centrifuge room if your centrifuge is equipped with a three wire power cord. Then wait
at least three minutes and then remove the covers from the centrifuge.
2) Be sure to turn off the POWER switch and unplug the power cord from the receptacle if
your centrifuge is equipped with a power cord with a plug. Then wait at least three
minutes and then remove the covers from the centrifuge.
(Section 3-5, Chapter 4, Chapter 5, and Chapter 6)
2.Indication of WARNING
1. The overspeed decal must match the maximum speed of the rotor, because the
overspeed decal is a critical component that detects the over-speed of the rotor: If an
overspeed decal that is not compatible with the rotor is attached, the rotor could break,
resulting in damage to the ultracentrifuge. (Section 2-2-4)
1. This centrifuge is not explosion proof centrifuge. Never use explosive or flammable
samples or materials that chemically react vigorously. Do not centrifuge such materials in
this instrument nor handle or store them near the instrument.
2. Take all necessary safety measures before using samples that are toxic or radioactive, or
blood samples that are pathogenic or infectious. (Section 3-1)
Do not incline or move the instrument while the rotor is spinning. Do not place any object on
the instrument or lean on the instrument. (Section 3-2)
The centrifuge itself may move if the rotor fails during high-speed rotation. Make sure there
is a 30 cm area around the centrifuge that will allow for such movement and do not allow
individuals to enter into that area during operation. Also do not place dangerous objects such
as flammable or explosive materials on top of the centrifuge or in the surrounding area.
(Section 3-2 and Chapter 6)
1. Never open the door during rotation.
2. Never touch the rotor during rotation. (Section 3-5)
It can take more than three hours for the rotor to come to a complete stop because the rotor
chamber is under vacuum. Before opening the door, wait until the rotor comes to a stop.
(Section 3-5)
Never touch the rotor while it is rotating. (Section 3-5)
1. If the centrifuge, rotor, or an accessory is contaminated by samples that are toxic or
radioactive, or blood samples that are pathogenic or infectious, be sure to decontaminate
the item according to good laboratory procedures and methods.
2. If there is a possibility that the centrifuge, rotor, or an accessory is contaminated by
samples that might impair human health (for example, samples that are toxic or radioactive,
or blood samples that are pathogenic or infectious), it is your responsibility to sterilize or
decontaminate the centrifuge, rotor, or the accessory properly before requesting repairs
from a Thermo Fisher Scientific authorized sales/service representative.
v

SAFETY NOTICES
3. It is your responsibility to sterilize and/or decontaminate the centrifuge, rotor, or parts
properly before returning them to a Thermo Fisher Scientific authorized sales/service
representative. In such cases, copy the decontamination sheet at the end of this manual and
fill out the copied sheet, then attach it to the item to be returned.
Thermo Fisher Scientific may ask you about the treatment for the centrifuge, rotor or the part
if the decontamination is checked and judged as insufficient by Thermo Scientific. It is your
responsibility to bear the cost of sterilization or decontamination.
(Chapter 4 and Chapter 5)
Unspecified repairs, remodeling or disassembly of the centrifuge that is not listed in Table 5-1
is strictly prohibited by any person other than a Thermo Fisher Scientific authorized
sales/service representative. (Section 5-1)
Before changing the power voltage by manually selecting desired tapping on the internal
transformer, turn off power to the ultracentrifuge, then unplug the power cord from the wall
outlet. Changing the voltage without doing so exposes you to the possibility of electrical
shock. (Chapter 6)
Your ultracentrifuge must be grounded properly. (Chapter 6)
Do not touch the power cord with wet hands to avoid electrical shocks. (Chapter 6)
Do not hold the cord when disconnecting the power cord from the receptacle. Hold the plug
when disconnecting it. (Chapter 6)
3.Indication of CAUTION
1.When replacing the overspeed decal with a new one, be very careful not to damage the
overspeed decal and rotor body. (Section 2-2-4)
Do not place containers holding liquid in the rotor chamber, on the centrifuge, or near the
centrifuge. Liquid, if spilt, could get into the instrument and damage electrical and mechanical
components. (Section 3-1)
1. Do not press the touchscreen with a sharp-pointed object such as a ballpoint pen.
2. If abnormal sound or vibration occurs, stop the operation immediately and contact a
Thermo Fisher Scientific authorized sales/service representative. (Section 3-2)
If you have selected a rotor in the Rotor Management screen, be sure to check that the
selected rotor (in the Rotor Management screen) and the rotor that is installed in the
centrifuge have an identical serial number. (The rotor serial number is also displayed in the
Rotor indicator field in the Run screen.) If the serial number of the selected rotor differs from
the serial number of the rotor installed in the centrifuge, you cannot control total run time and
the total number of runs of both rotors. (Section 3-2-3)
Take care not to get your hands or fingers caught in the door of the centrifuge.
(Section 3-2-5)
vi

SAFETY NOTICES
Zonal centrifugation includes operations where the rotor will turn while the door is open. Be
sure to read though the “Zonal rotor instruction manual” before operation. (Section 3-4-3)
The cap is installed by hand on the rotor when it is turning. Perform this operation with care
and be sure to follow the instructions in the instruction manual. (Section 3-4-3)
The cap is removed by hand from the rotor and the seal assembly is installed when the rotor
is turning. Perform this operation with care and be sure to follow the instructions in the
instruction manual. (Section 3-4-3)
Never conduct operations in a manner other than as described in this operation manual.
(Section 3-5)
When the centrifuge will not be used for a long time, keep the power breaker off.
(Section 3-5)
Do not perform any operation not specified in this manual. If any problem is found on your
centrifuge, contact a Thermo Fisher Scientific authorized sales/service representative.
(Chapter 4 and Chapter 5)
Using cleaning or sterilization method other than recommended in this instruction manual
may cause corrosion or deterioration of this centrifuge. Refer to chemical resistance chart
attached to the rotor or contact Thermo Fisher Scientific. (Chapter 4)
For sterilization of the surface of the centrifuge and the rotor chamber, wipe them with a cloth
dampened with 70% ethanol. Using the method other than the above method might cause
corrosion or deterioration of the centrifuge. Refer to the chemical resistance chart provided
with the rotor, or contact Thermo Fisher Scientific. While we recommend that 70% ethanol
is used for sterilization, no guarantee of sterility or disinfection is expressed or implied.
When sterilization or disinfection is a concern, consult your laboratory safety officer
regarding proper methods to use. (Chapter 4)
Do not pour any solution such as water, detergent and disinfectant directly into the rotor
chamber. Otherwise, the bearings of the drive unit may be corroded or deteriorated.
(Section 4-1)
Clean the inside of the drive hole (crown hole) of the rotor and the surface of the drive shaft
(crown) of the centrifuge once a month. If the drive hole or the drive shaft is stained or any
foreign matter is adhered, the rotor may be improperly installed and come off during
operation. (Section 4-2)
Your ultracentrifuge can be damaged if connected to a wrong voltage. Check the voltage
before plugging the ultracentrifuge into a power source. (Chapter 6)
Be sure to remove the rotor from the rotor chamber when centrifuge is moved. After
installation and before any test-run, this ultracentrifuge always needs the internal check by a
Thermo Fisher Scientific authorized sales/service representative. (Chapter 6)
vii

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AVERTISSEMENT
Ne pas ouvrir le couvercle de la
chambre à air durant la rotation.
Si vous êtes confrontés à une panne
de courant, veuillez vous référer aux
instructi ons du manuel.
S310564
 WARNING
Do not loosen the vacuum release
screw during rotation. In the event of a
power outage, operate according to the
instruction manual.
AVERTISSEMENT
Ne ja mais uti lise r de m atériau capable de produire
des vapeurs inflammables ou explosives.
Assu rez-vous de prendre les précautions
nécessaires avant de trier des matières
radioactives, toxiques ou pathogènes.
S310568
 WARNING
Never u se any material capable of producing
flammable or explosive vapors.
Be sure to take the necessa ry precautions before
separating radioactive, to xic o r pathog enic
materials.
Risque de choc électrique
.
Débranchez le cordon
d'alimentation avant
l'entretien et attendre au
moins trois minutes.
AVERTISSEMENT

Risk of electrical shock.
Disconnect equipment
from power supply
before servicing and wait
for at least three
minutes.
WARNI NG
S31056
6
 CAUTION
T h i s co v e r should only be removed for
power outages or other situations that
are absolutely necessary. Always ma ke
sure that the power source is off and
follow the instructions in the manual.
 ATTENTION
Ce couvercle doit uniquement être
retiré en cas de panne de courant et
d'autres situations qui rendent la
manipulation absolument nécessaire.
Assu rez-vou s toujours que la source
d'alimentation est coupée et suivez
les inst ructions du manuel.
S3105582