Thermo Scientific Thermo Scientific Savant SpeedVac DNA120 User manual

Instruction Manual
Thermo Scientific Savant®DNA120,
DNA120 OP SpeedVac®Concentrators

©2008 Thermo Fisher Scientific. All rights reserved.
“Suva®” is a registered trademark of DuPont.
All other trademarks are the property of Thermo Fisher Scientific Inc. and
its subsidiaries.

Table of Contents
Section Section Title — Introduction Page
1.0 Introduction 1
Section Section Title — Installation Page
2.0 Installation 1
Receiving 1
Unpacking 1
Inspection 1
Set-up 2
Section Section Title — Operation Page
3.0 Operation 2-3
3.1 ode Selection 4
3.2 Setting Target Time 4
3.3 Example of anual Run 5
3.4 Example of Auto Run 6
3.5 Drying Rate 6
3.6 Other Topics 7
Section Section Title — Specifications Page
4.0 Specifications 8
Section Section Title — Accessories Page
5.0 Accessories 9
Appendix 1:
Proper handling of hazardous exhaust vapors 10
Appendix 2:
Operating hints 10

1
1.0 I TRODUCTIO
TheThermo Scientific Savant®DNA SpeedVac®odel DNA120, is a dedicated
centrifugal vacuum concentration system for drying low volume ethanol or
isopropanol-water precipitates of DNA and RNA. The Thermo Scientific Savant
DNA120 OP allows for additional drying down of synthetic oligos in Ammonium
Hydroxide (NH4OH). This design incorporates a glass cover, ammonia post-trap
and Ammonia Neutralizing Solution for fully automated, unattended, odor-free
drying. The post-trap is connected on the back, left side of the unit. It is easily
maintained and replaced. The Thermo Scientific Savant DNA SpeedVac
combines centrifugal force with vacuum for bump-free sample drying. Vacuum is
supplied by an integral, oil-free vacuum pump with an automatic bleeder valve.
The Thermo Scientific Savant DNA120 SpeedVac has a chamber heater that
counteracts evaporative sample cooling and accelerates solvent evaporation
rates to shorten drying times. The operator can select LOW (ambient),
EDIU (43 °C), or HIGH (65°C) drying rates with the 3-position switch on the
front panel.
Several different microcentrifuge tube rotors are available for drying ethanol
precipitates of DNA/RNA, isopropanol-water mixtures, amplified reaction
products in buffer, aliquots of radiochemicals, or other low-volume samples (see
section 5). A unique swing-out rotor (RD2 P) for microplates is available as
well.
The Thermo Scientific Savant DNA SpeedVac is equipped with polypropylene
vacuum fittings to avoid leaks and resist corrosion. A chemical trap kit
(DTK120R) and disposable cartridge (DC120R) should be used for trapping
volatile radioactivity when the system is dedicated for drying down radiolabeled
materials.
NOTE:To assure safe operation and best results, read this manual in its
entirety before operating this instrument.
RECEIVI G Inspect the shipping carton upon receipt. If the carton is
damaged in anyway, do not accept delivery. Contact Thermo
Scientific.
U PACKI G Carefully remove the instrument from its shipping carton.
Compare the packing list to the box contents. If there is a
discrepancy contact Thermo Scientific.
I SPECTIO Inspect the unit for any damage that may have occurred
during shipment. Should there be any damage, report it
to the carrier and contact Thermo Scientific immediately.
ake sure the carrier inspects the damage and leaves an
inspection report. Register any claims for shipping damage
against the carrier or his agent. Save the shipping carton in the
event a return is necessary.

2
Approximate Drying Times
Solvent Volume/ Number Time to dryness at drying rate:
Tubes of tubes LOW EDIU HIGH
Ethanol 0.5 ml 12 60± 5 min. 35± 5 min. 25± 5 min.
Table 1: Ethanol drying in the Thermo Scientific Savant DNA SpeedVac. Drying times
presented as a guide only. Each system may vary slightly. The solvents used, and type
and quantity of solutes, also effect drying time. Thermo Scientific Savant DNA SpeedVac
Test Conditions: RD24 rotor with 12 x 1.5 ml tubes.
2.0 I STALLATIO (cont’d)
SET-UP
• Install the system on a stable surface that is clean, dry, level, and
within 4 feet (1.2 meters) of a compatible electrical outlet.
• Connect power cord to receptacle on the left side of the instrument
and plug into appropriate outlet. The cover latch mechanism releases
automatically.
WAR I G: Before connecting the D A120 to an outlet, make certain that
voltage,frequency, and amperage match the D A120
requirements indicated on the label on the left-hand side of the
instrument (120VAC / 60 Hz, 10 A or 230 VAC / 50 Hz, 5 A).
If there are any questions, please consult an electrician.
• Place rotor on the drive shaft in the sample chamber. Hand-tighten
the hold-down knob until firmly seated.
CAUTIO : Use only Thermo Scientific designed rotors in the
Savant D A SpeedVac®.
•For 230 VAC / 50 Hz, 5 A units only: A safety latch prevents
the Savant DNA SpeedVac lid from operating until power is applied to
the system. Do not attempt to open the lid unless the unit is
plugged in.
3.0 OPERATIO
The switches on the front panel control operation of the Thermo Scientific Savant
DNA SpeedVac. The LED readout displays “run” parameters in minutes. You may
choose between three drying rates (LOW/ EDIU /HIGH).

3
A
D
B
C
3.0 OPERATIO (cont’d)
ROTOR I STALLATIO . Open the lid of the rotor chamber. Carefully lower the
rotor onto the drive shaft. Secure the assembly by screwing the
retaining knob into the drive shaft above the rotor. Tighten it firmly but not
excessively. Load the rotor and close the lid. Always balance rotor loads. There
need not be a tube in each hole, but the load must be evenly
distributed.
USI G THE CO TROL PA EL
A. OPERATI G MODE SWITCH (see section 3.1)
AUTO Press to start a run with automatic shut-off
OFF Press to stop any run
ANUAL Press to start a run with manual shut-off
B. TIMER SELECTIO SWITCH (see section 3.1)
HEATER Press to select Heater timer
RUN Press to select Run timer
C. CHA GE SETTI G SWITCH (see section 3.2)
Press to increase value of timer selected by switch B
Press to decrease value of timer selected by switch B
D. DRYI G RATE SWITCH (see section 3.6)
LOW Concentrator chamber at ambient temperature
EDIU aintains chamber at 43°C
HIGH aintains chamber at 65°C

Actual elapsed time can be viewed during a run or at the end of a run. When
switch A is set to “OFF”, both timers are at 0.00 (0 hours and 0 minutes). You can
change the settings only when switch A is “OFF”; settings cannot be changed
during a run.
Set switch B to “RUN” to view or change the run timer. Set switch B to “HEATER”
to view or change the heater timer.
When you do not set a run time the timer counts upward (time elapsed). When a
time limit is set, the timer counts down; the display indicated the time remaining.
Using switch C increases or decreases the time setting in 5 minute increments to
to 9.55 (9 hours, 55 minutes) holding down switch C allows you to rapidly scroll
thru the settings until you release the switch.
• When setting the heater timer, above 9.55 (or below 0.05) there
exists a setting of “CCC” (for continuous HEATER operation),
and “OFF” (for HEATER off).
4
3.1 MODE SELECTIO
The dual timer front panel permits operation of the Savant DNA120 in one of the
two modes. Select switch A to either:
•MA UAL-to initiate a concentrator run that you can start and stop
manually. The run starts when switch A is set to “ ANUAL” when
switch A is set to “OFF”. The unit will end the concentrator run.
or
•AUTO-to start a concentrator run for a predefined time interval. The
run starts when switch A is set to“AUTO”. The run stops
automatically at the end of the predefined time selected. The front
panel maintains two independent timers (switch B).
•RU -timer measures the duration of the current run. You can view
or change this timer, (using switch C) when switch B is set to “RUN”.
Each of the above consists of a user-specified target setting and a timer of actual
elapsed time.
A three-digit display shows hours and minutes. A decimal point separates hours
from minutes. For example, 9.55 indicates 9 hours and 55 minutes. The display
can be set to show either the setting or the elapsed time.
The decimal point flashes when a run is in progress (auto run or manual run). The
decimal point is always lit when a run is not in progress.
3.2 SETTI G A TARGET TIME

Switch A should be “OFF”.
Set switch B “HEATER” to bring the HEATER time to the display. Adjust switch C
until the display shows the desired setting for HEATER. You can select:
• “OFF”-to disable the HEATER.
or
• “CCC”-to operate the HEATER for the duration of the run.
or
• A specified length of time. The HEATER switches off at the end
of this time interval. If a longer time than specified run time is
specified, then the HEATER shuts off at the end of the run.
Start the run by setting switch A to “ ANUAL”.
During the run, you can toggle the unit to display the following information.
• Elapsed run time. Set switch B to “RUN”. Since there is no
present run time, the display counts upward, showing the hours
and minutes that have elapsed in the current run.
or
• Remaining heating time. Set switch B to “HEATER”. If a
specific time setting for the HEATER is selected, then the
display counts down and shows it.
3.3 EXAMPLE OF A MA UAL RU
5

Switch A should be “OFF”.
Before starting the Auto Run, specify, a run time as follows:
Set switch B to “RUN”. Adjust switch C until the display shows the desired duration
of the run in hours and minutes.
Start the run by setting switch A to “AUTO”.
During the run, you can toggle the unit to display the following information:
• Remaining run time. Set switch B to “RUN”. The display now
counts downward, showing the hours and minutes of time left
in the current run.
or
• Remaining heater time. Set switch B to “HEATER”. If a specific
time setting is selected for the HEATER, then the display counts
down and indicates the amount of time remaining before the
HEATER shuts off.
To terminate a run, set switch A to “OFF”.
3.4 EXAMPLE OF A AUTO RU
Thermal energy can be applied to the concentrator chamber to counteract the
cooling effect of evaporation, it maintains samples in the liquid state, and
accelerates the concentration run. The DRYING RATE switch selects the desired
drying rate:
“LOW” maintains the concentrator chamber at ambient temperature.
“MEDIUM” maintains the concentrator chamber at 43° C.
“HIGH” maintains the concentrator chamber at 65° C.
Select the highest drying rate if you desire accelerated drying. To ensure against
overdrying of samples, and possible denaturing, it is recommended that the
HEATER timer be set for only a portion of the total run time (e.g., 50%-75% of total
run time).
3.5 DRYI G RATE
6

Input power: 120 VAC / 60 Hz, 10A or
230 VAC / 50 Hz, 5A
Vacuum Chamber: TEFLON®coated aluminum casting
Cover: • Transparent acrylic (DNA120) 230 VAC / 50 Hz
unit include a safety interlock
• Glass cover (DNA120 OP) w/ safety interlock
Drive & Speed: aintenance-free induction motor; 1725 RP with
magnetic coupling to rotor drive shaft
RCF approximately 300 xg
Capacity: 24 x 1.5 ml tubes (RD24 rotor)
36 x 1.5 ml tubes (RD36 rotor) or
48 x 0.5 ml tubes (RD48 rotor)
72 x 0.5 ml tubes (RD72 rotor) or
2 microplates (RD2 P rotor)
Chamber temperature: Controlled at ambient, 43° C,
or 65° C with over-temperature safety shut-off
Weight: 86 lbs. (39 kg)
Dimensions (W x D x H): 11 in. x 25 in. x 11 in.
(29 cm x 63 cm x 29 cm)
Pump: oil-free diaphragm
Displacement: 36 l/min at 60 Hz
30 l/min at 50 Hz
Ultimate Vacuum Level: 7 torr (9 mbar)
Registration: ISO 9001-1994. Registered by Q I (Quality
anagement Institute). Certificate number 001080.
4.0 SPECIFICATIO S (Subject to change without notice)
7