Thermo Scientific CryoStar NX50 Manual

COMPANY INFORMATION ...............................................................................................................................3
EMC STATEMENT ................................................................................................................................................... 4
IMPORTANT NOTE REGARDING INSTRUMENT VARIANTS ................................................................................5
SYMBOLS .........................................................................................................................................................6
TABLE OF CONTENTS........................................................................................................................................8
CHAPTER 1 - SAFETY INFORMATION ..............................................................................................................11
GENERAL SAFETY................................................................................................................................................... 11
CHEMICAL SAFETY ................................................................................................................................................. 12
ENVIRONMENT ..................................................................................................................................................... 12
WARRANTY STATEMENT ......................................................................................................................................... 13
CHAPTER 2 - INTRODUCTION .........................................................................................................................14
INTENDED USE...................................................................................................................................................... 14
INSTRUMENT FEATURES.......................................................................................................................................... 14
TECHNICAL SPECIFICATIONS..................................................................................................................................... 15
Mechanical Specifications............................................................................................................................ 15
Electrical Specifications................................................................................................................................ 15
Environmental Specifications ....................................................................................................................... 15
Cooling Specifications .................................................................................................................................. 16
Microtome and Sectioning Specifications .................................................................................................... 16
Cold D Specification...................................................................................................................................... 17
Default Settings............................................................................................................................................ 17
CHAPTER 3 - INSTRUMENT SETUP ..................................................................................................................18
UNPACKING AND REPACKING................................................................................................................................... 18
Safety Precautions ....................................................................................................................................... 18
Unpacking Procedure................................................................................................................................... 18
Repacking the Instrument............................................................................................................................ 19
LOCATION &SETUP ............................................................................................................................................... 20
IDENTIFICATION OF PARTS....................................................................................................................................... 21
Overview ...................................................................................................................................................... 21
Connections Panel........................................................................................................................................ 21
Cryochamber................................................................................................................................................ 22
Blade Holder................................................................................................................................................. 22
Specimen Head NX50 ................................................................................................................................... 22
Set Up........................................................................................................................................................... 23
Fitting or Exchanging the Anti Roll Plate...................................................................................................... 23
Fitting or Exchanging Low Profile Blades ..................................................................................................... 24
Removing Used Blades with the Magnetic Blade Tool................................................................................. 25
Blade Positioning and Orientation ............................................................................................................... 26
Adjusting the Cutting Angle ......................................................................................................................... 27
Adjusting the Anti-Roll Plate ........................................................................................................................ 28
Specimen Setup............................................................................................................................................ 28
FILLING WITH DISINFECTANT TO PERFORM THE COLD DFUNCTION (OPTIONAL)................................................................. 30

Filling the Cryostat with Disinfectant Solution (Sanosil®) ............................................................................ 30
CHAPTER 4 - OPERATION ...............................................................................................................................32
SWITCHING ON..................................................................................................................................................... 32
SWITCHING OFF.................................................................................................................................................... 32
SYSTEM INTERFACING............................................................................................................................................. 33
Using the Touchscreen ................................................................................................................................. 33
Main Screen ................................................................................................................................................. 33
Trimming and Fine Section Thickness .......................................................................................................... 35
Temperature ................................................................................................................................................ 36
Chamber Temperature ................................................................................................................................. 36
Cutting Window ........................................................................................................................................... 37
Knife Assembly Positioning .......................................................................................................................... 38
Quick Freezing of the Specimen ................................................................................................................... 39
Vacutome and Cleaning Mode (optional) .................................................................................................... 40
Height Adjustment (optional) ...................................................................................................................... 43
Multi-Function Bar ....................................................................................................................................... 43
Time and Date.............................................................................................................................................. 44
Multi-Function Button.................................................................................................................................. 44
Menu............................................................................................................................................................ 45
Language ..................................................................................................................................................... 45
Standby ........................................................................................................................................................ 46
Defrost ......................................................................................................................................................... 46
Immediate Defrosting .................................................................................................................................. 47
Programming the Daily Defrost Cycle .......................................................................................................... 48
Chamber Light.............................................................................................................................................. 49
Screen........................................................................................................................................................... 49
Display Lock.................................................................................................................................................. 50
Retraction .................................................................................................................................................... 51
Data Log....................................................................................................................................................... 52
Factory Settings ........................................................................................................................................... 53
Service .......................................................................................................................................................... 54
User Guide.................................................................................................................................................... 54
Time and Date.............................................................................................................................................. 55
SECTIONING PROCEDURES....................................................................................................................................... 58
Cutting Movement and Retraction .............................................................................................................. 58
Trimming and First Cuts ............................................................................................................................... 58
Trimming...................................................................................................................................................... 58
Sectioning and Taking Off Sections .............................................................................................................. 59
HAND BRAKE LOCK................................................................................................................................................ 60
Mechanical Hand Brake Lock ....................................................................................................................... 60
COLD D(OPTIONAL) .............................................................................................................................................. 61
Starting the Disinfection (Cold D) Function .................................................................................................. 61
Aborting the Disinfection ............................................................................................................................. 62
Programming Cold D.................................................................................................................................... 63
CHAPTER 5 - TROUBLESHOOTING ..................................................................................................................65
TROUBLESHOOTING TABLES..................................................................................................................................... 65
TIPS FOR SUCCESSFUL SECTIONING ........................................................................................................................... 67
How to avoid malfunctions when working with the Vacutome ................................................................... 68

Sectioning Temperature Guidelines for Fresh Tissues .................................................................................. 69
CHAPTER 6 - CLEANING AND MAINTENANCE .................................................................................................70
CLEANING THE TOUCH SCREEN DISPLAY..................................................................................................................... 70
SHUTTING OFF FOR CLEANING ................................................................................................................................. 70
CLEANING AND CARE OF THE BLADE HOLDER.............................................................................................................. 70
EMPTYING THE DEFROSTING AND COLD DLIQUIDS ...................................................................................................... 71
EXCHANGE THE VACUTOME FILTER BAG .................................................................................................................... 72
ADJUSTMENT OF THE MOVABLE SPECIMEN HEAD JAW ................................................................................................. 74
TRANSPORTATION OF THE INSTRUMENT..................................................................................................................... 75
Conditions for the Transportation of the Instrument................................................................................... 75
APPENDICES...................................................................................................................................................78
APPENDIX A-SPARES AND ACCESSORIES ................................................................................................................... 78
APPENDIX B-RECOMMENDED DISINFECTION PROCEDURE ............................................................................................ 81
APPENDIX C–COLD DISINFECTION CERTIFICATE ......................................................................................................... 82
INDEX ............................................................................................................................................................83

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