Thermo 1284 Operating instructions

Model 1284 Series
Class II, A2 Biological Safety Cabinet
Operating and Maintenance
Manual 7021284 Rev. 3

Thermo Electron CorporationThermo Electron Corporation
MANUAL NUMBER 7021284
3 23429 4/3/06 Updated low flow alarm from 191168 to 191780 ccs
2 23059/HD-1472 1/17/06 GFI reset switch reminder aks
-- -- 6/20/05 Added UV replacement info to Page 9-3 ccs
-- 21497/HD-1424 9/8/04 Added factory-installed anchoring system accessory ccs
-- -- 6/7/04 Clarify test pages ccs
1 22280 4/20/04 Update to incorporate new constricted testing method aks
22197 4/8/04 Blower motor replacement aks
-- 21984 11/13/03 Updated 1284 test sheet (added “four inches above window edge”) ccs
0 21741/HD-1405 8/7/03 Release 6 aks
REV ECR/ECN DATE DESCRIPTION By
Preface
Model 1284 Series i
Important Read this instruction manual. Failure to read, understand and
follow the instructions in this manual may result in damage to the unit,
injury to operating personnel, and poor equipment performance. ▲
Caution All internal adjustments and maintenance must be performed by
qualified service personnel. ▲
The material in this manual is for information purposes only. The contents
and the product it describes are subject to change without notice. Thermo
Electron Corporation makes no representations or warranties with respect
to this manual. In no event shall Thermo be held liable for any damages,
direct or incidental, arising out of or related to the use of this manual. ▲
Caution Lamps, thermometers and thermoregulators contain mercury. Do
not put in trash! Recycle or dispose as hazardous waste. ▲.

Thermo Electron Corporationii Model 1284 Series Thermo Electron Corporation
Preface
Caution Alerts the user to important operating and/or maintenance
instructions. May be used alone or with other safety symbols. Read the
accompanying text carefully. ▲
Caution Potential electrical hazards Only qualified persons should perform
the instructions and procedures associated with this symbol. ▲
Hazard Do not touch. Instructions associated with this symbol should
only be carried out when using special handing equipment or when
wearing special, protective clothing. ▲
Potential biological hazards Proper protective equipment and procedures
must be used when following instructions associated with this symbol.
Reference O.S.H.A. Regulation 1910-1030. ▲
Potentially hazardous energy Equipment being maintained or serviced
must be turned off and locked off to prevent possible injury. Reference
O.S.H.A. Regulation 1910-147. ▲
Caution Hot surface(s) present which may cause burns to unprotected skin
or to materials which may be damaged by elevated temperatures. ▲
Warning Skin damage and/or eye injury can result from the light
produced by ultra violet light sources installed in this equipment. Never
work in this unit with the ultra violet light operating. ▲
Marking of electrical and electronic equipment, which applies to electrical
and electronic equipment falling under the Directive 2002/96/EC
(WEEE) and the equipment that has been put on the market after 13
August 2005.
This product is required to comply with the European Union’s Waste
Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is
marked with the WEEE symbol. Thermo Electron has contracted with one
or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them.
Further information on Thermo Electron’s compliance with this directive,
the recyclers in your country and information on Thermo Electron
products will be available at www.thermo.com.
Always use the proper protective equipment (clothing, gloves, goggles, etc.)
Always dissipate extreme cold or heat, or wear protective clothing.
Always follow good hygiene practices.
Each individual is responsible for his/her own safety.
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Thermo Electron Corporation Model 1284 Series iiiThermo Electron Corporation
Preface

Model 1284 Series ivThermo Electron Corporation
Table of Contents
Packing List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Plumbing Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Exhaust Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Certification Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Locating a Certification Company . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
General Cautions and Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Cabinet Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Use of Auxiliary Equipment in Cabinet . . . . . . . . . . . . . . . . . . . . . . .7-1
Cabinet Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Start-Up Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6
Section 7

v Model 1284 Series Thermo Electron Corporation
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Zeroing the Static Pressure Gauge . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Checking Static Pressure Gauge “Zero” . . . . . . . . . . . . . . . . . . . . . . .9-1
Adjusting the Damper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-2
Biological Safety Cabinet Test Grids . . . . . . . . . . . . . . . . . . . . . . . . . .9-3
Blower Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-5
Receptacle Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-5
UV Switch Adjustment Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . .9-6
Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Replacing Blower Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Replacing Filters, All Models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-2
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-1
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11-3
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12-1
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13-1
Filter Pressure Drop Conversion . . . . . . . . . . . . . . . . . . . . . . . . . . .13-3
Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14-1
Warranty Information
Table of Contents
Section 8
Section 9
Section 10
Section 11
Section 12
Section 13
Section 14

Model 1284 Series 1-1Thermo Electron Corporation
Section 1 Packing List
Included with the installation/operation manual are four identification
index buttons. These buttons may be used to identify the type of service
supplied to the service valves. Also included in a separate bag is the drain
valve. Attached to the valve is a small Allen wrench used for calibrating the
Static Pressure Gauge. This Allen wrench should be kept with the manual
at all times.

Model 1284 Series 2-1Thermo Electron Corporation
Section 2 Introduction
The Models 1284, 1285, 1286 and 1287 are Class II, Type A2 cabinets.
The “Type A2” designation indicates two alternative uses of the cabinet.
The unit may be vented directly into the laboratory room or vented to the
outside atmosphere, through an in-house exhaust system. Either usage of
the cabinet offers both personnel and product protection.
The cabinet can be used in low-to-moderate risk environments and is
designed to NSF, International Standard #49. Class 1, 2, and 3 (low-to-
moderate risk) agents are described in the “Biosafety In Microbiological
And Biomedical Laboratories”; CDC NIH Publication No. (NIH) 88-
8395, March 2002.
The cabinet’s window permits the user to place auxiliary equipment and
research implements in the work area. The work opening must be held to
10 inches during all work procedures. If the window is raised higher than
the designated 10 inches, the air barrier at the front of the cabinet will be
weakened and containment will be seriously impaired.
Clean, filtered air descends through the work zone with approximately
40% being discharged through the exhaust HEPA filter with the remaining
air recirculating through the supply HEPA filter into the work area.
Exhausted air is replaced by room air entering the system through the front
access opening.
Room air entering the work zone, through the front access opening,
completes the air barrier at the unit face and is responsible for the
containment properties of the unit. All work must be performed beyond
the intake grille, on the solid work tray.
Theory of Operation

Model 1284 Series 3-1Thermo Electron Corporation
Section 3 Installation
Locate the cabinet on a firm, level surface in an area of minimum
temperature changes. The cabinet should be placed away from disruptive
air currents caused by excessive personnel traffic, air-conditioning or
heating ductwork, or laboratory windows and doors. Proper cabinet
location is important, as drafts disrupt critical airflow characteristics and
allow room contaminants to enter or escape the cabinet work area.
Where space permits, fourteen inches should be allowed on each side of
the cabinet for maintenance. A twelve-inch height should be available from
the top of the cabinet to the ceiling.
Place a bubble-type level on the work surface. Adjust the leveling feet until
the cabinet is level and the most comfortable working height is achieved.
Ensure that all four leveling feet are fully flush against the floor to prevent
vibration.
Warning Model 1284/1285 weighs 735 lbs. Model 1286/ 1287 weighs
940 lbs. Have sufficient personnel available to lift it. ▲
Location
Figure 3-1. Power Switch Location
Power Switch

3-2 Model 1284 Series Thermo Electron Corporation
Section 3
Installation
Power Connection The power switch is located on the left side of the control panel (Figure 3 -
1). The power switch is also a circuit breaker that protects the unit. The
electrical wall outlet leading to the cabinet should be accessible for
electrical testing.
This cabinet is equipped with one power cord supplying power to the
blower, lights and receptacles. The cord should be plugged into a
grounded, dedicated circuit. Refer to Section 11, Specifications, or to the
serial plate on the front of the unit for electrical specifications. With the
power switch turned off, plug the line cord into the wall outlet.
All models have one standard service valve located on the right side of the
work station. Service valves are piped within the cabinet. External
connection is a 3/8” FPT coupling. Identification index buttons are
supplied. Each cabinet can accommodate a total four service valves.
Explosive/flammable substances should never be used in the cabinet, unless
approved and monitored by a biological safety officer or other qualified
individual. However, if flammable gas is used, emergency shut-off valves
must be located in an accessible area external to the cabinet.
The Universal Plumbing option is factory installed. External connection
(1/4” NPT) to the unit is available on the top and the underside of the
cabinet, as well as the standard side connection. See Figure 3-2.
Plumbing Connection
Universal Plumbing
Option
Figure 3-2. Universal Piping

Model 1284 Series 3-3Thermo Electron Corporation
Section 3
Installation
Filtered air from the cabinet may be exhausted directly into the room or
vented to the outside through an external exhaust system. Consult a
biological safety officer or other qualified individual for cabinet-type
exhaust requirements. Refer to NSF Standard, NSF 49-2002, Annex E.
A cardboard cover plate is shipped under the exhaust filter guard. It must
be removed before the unit is placed into service. Locate the exhaust filter
guard on the top of the cabinet. Remove the guard and discard the
cardboard. Secure the exhaust filter guard as previously.
If an external exhaust system is needed, use a canopy (thimble) connection
(Figure 3-3). When the cabinet is certified, check the opening in the
canopy to ensure inward airflow, using a smoke stick. Ensure proper air
balance at the front access opening for adequate containment. Verify that
the building exhaust system is sized to exhaust 30% more air than the
cabinet exhausts. Models 1284 and 1285 exhaust an air volume of 358-
391 CFM. Models 1286 and 1287 exhaust an air volume of 531-580
CFM.
Warning The exhaust air must be drawn from the cabinet through a
dedicated exhaust system (only one BSC per exhaust system). The exhaust
system may be connected to the collar (optional exhaust transition) located
on the top of the unit.
The exhaust system should have safeguards against exhaust failure. It is
required that a biological safety officer, industrial hygienist or other
qualified individual review the agents and chemicals used inside the
cabinet to determine if additional filtration treatment is necessary before
venting to the atmosphere. ▲
Canopy/Thimble style - P/N 191570 Release 1-5 units, P/N 191782
Release 6 units. Refer to the unit serial tag to determine release level.
4 foot models - Nominal 375 CFM Direct method and calculated method.
Nominal 487 CFM, when sizing to exhaust 30% more air (than the cabi-
net exhausts)
6 foot models - Nominal 556 CFM
Nominal 722 CFM, when sizing to exhaust 30% more air (than the cabi-
net exhausts)
Exhaust
Requirements
Direct Room Exhaust
External Exhaust System
(for Class II Type A2)
Optional Exhaust
Transition
Nominal Exhaust
Requirements

3-4 Model 1284 Series Thermo Electron Corporation Section 3 Installation
Figure 3-3. Transition Specs

Model 1284 Series 4-1Thermo Electron Corporation
Section 4 Certification Testing
Caution Service and certification must be performed by qualified
personnel. ▲
Biological safety cabinet certification consists of a series of tests designed to
verify that the cabinet is performing within operating parameters
established by the manufacturer.
To assure that a biological safety cabinet is operating as intended, each
cabinet should be field-tested at the time of installation and at least
annually thereafter. Cabinets should be re-certified whenever HEPA filters
are changed, internal maintenance is performed, or is relocated.
Three industry-related organizations maintain lists of companies and
individuals who are active in the certification industry. You may contact
these organizations at the addresses listed below.
NSF International (NSF) and International Air Filtration Certifiers
Association (IAFCA) sponsor certifier accreditation programs. Accredited
certifiers have demonstrated proficiency at testing biological safety cabinets
by successfully completing written and/or practical examinations.
Biohazard Cabinet Field Certifier Program
NSF International
PO Box 130140
789 N. Dixboro Rd
Ann Arbor, MI 48113-0140
Telephone [phone] Or (800) NSF-MARK
Fax [phone]
http://www.nsf.org/Certified/Biohazard-Certifier
Locating a
Certification Company

4-2 Model 1284 Series Thermo Electron Corporation
Section 4
Certification Testing
IAFCA
PO Box 12155
Columbus, OH 43212
Telephone [phone]
Fax [phone]
http://www.iafca.com/certifier.html
The Controlled Environment Testing Association (CETA) is a trade
association devoted to promoting and developing quality assurance within
the controlled environment testing industry. A list of active members is
available by contacting the organization.
Controlled Environment Testing Association
1500 Sunday Drive
Suite 102
Raleigh, NC 27607
Telephone [phone]
Fax [phone]
http://www.cetainternational.org/members/corp_indiv.htm
For your convenience we have included a partial list of agencies that
perform certification on our website. Select Certification Companies
located under the Services and Support heading at
www.thermo.com/forma. If you do not find someone listed in your area,
please contact the Technical Services Department for additional references.
Note Unless certification was expressly called for in the specification,
quotes and/or purchase order, the cost for this on-site testing is to be paid
for by the customer. ▲

Model 1284 Series 5-1Thermo Electron Corporation
Section 5 Operation
Before operating the cabinet, become familiar with the following items:
Blower Switch- The blower switch controls power to the internal blower.
Light Switch- The “Lights” switch controls power to the fluorescent lamp
or the optional ultraviolet lamp. Both lamps are located in the work area.
Ultra-Violet Light - Optional
Warning On cabinets equipped with ultra violet lights, the lights must be
turned off when working in the unit. Potential eye damage may result from
viewing the light produced by ultra violet sources. The UV interlock on
this cabinet prevents ultra violet illumination when the sliding window is
raised. Make sure the sliding window is within approximately 3/8” of the
fully closed position. ▲
On cabinets equipped with the optional ultra-violet germicidal light, the
dual purpose “Lights” switch provides the following settings:
“Off” = center
“On” top = fluorescent lamp
“On” bottom = ultra-violet lamp on with the window completely down.
Note Either the fluorescent lamp or the ultra-violet lamp may be lit at one
time. ▲
Alarm By-pass Switch - The Alarm By-pass switch silences the audible
“Window Above 10 Inch” alarm for approximately five minutes. The red
visual indicator remains illuminated. The alarm rings-back to remind the
operator that the window is still open more than 10 inches.

5-2 Model 1284 Series Thermo Electron Corporation
Section 5
Operation
Static Pressure Gauge (In. W.G.) - The static pressure gauge measures the
air pressure differential across the filters providing an indication of filter
“loading”. As the filters become loaded, resistance increases and the reading
on the static pressure gauge increases accordingly. When the reading
increases by 50% from original measurement, cabinet airflow should be
checked with a thermoanemometer. Replace the filters if proper airflow
cannot be obtained.
Note The static pressure gauge should not be used as a direct measure of
airflow. ▲
Figure 5-1. Control Panel
Figure 5-2. Front View of 4 ft. Unit with Components Indicated

Power Switch - mains disconnect/circuit breaker for the cabinet.
Blower Motor/Lights Reset Button (15 Amp) - The Reset button (located
on the left side of the control panel, directly above the Receptacle fuses) is
an in-line circuit breaker for the internal blower motor and lighting. If an
overload occurs, the circuit breaker will trip and the button will protrude
from the panel. Depress the button to reset the circuit breaker.
Note • Turn power off to the blower.
• Turn power off to the lighting.
• Press the blower motor/lighting Reset button. ▲
Receptacles - Receptacles (115 Volts) are
located on the left and right sidewall of the
workstation. Power is controlled by the
receptacle switch located on the control
panel. The maximum load is 5 amps total
for Models 1284 and 1285. Models 1286
and 1287 have a maximum load of 2 amps
total. Models 1285 and 1287 are equipped
with 2 single European 230V receptacles.
Model 1284 Series 5-3Thermo Electron Corporation
Section 5
Operation
Figure 5-3. Side View of 4 ft. Unit with Components Indicated
Figure 5-4. Locations