Thermo Centra CL2 User manual

Instruction Manual
IEC Centra CL2
Centrifuges
Cat. No.426: 120 VAC, 50/60Hz
Cat. No.427: 240 VAC, 50/60Hz
IM-426 Rev. 10 11 January 2004

Contents
TRTINEOAUCHaNI.....-.. een 3
2. Installation rin 5
2.1 Reeeiveithe URL ニー SL テラ ミー ニー ドー ンー ドーーーー 5
2.2 Prepare the installation site................... των 5
2.3 VerifyPowerConfiguration.......................................... 5
24Movingthelnmit..................................... 5
3; Operar nn 6
3.1 Warnings and Cautions.................... iii 6
3.2 Opening The Cover... His PNEUS 6
3.3 Rotor Installation ...................... inn 6
3.4 Starting and Stopping à RUN... een 6
3.5 Rotor Removal ose one nesen enénéce 6
人 7
4. Applications
4.1 Introduction
4.2 Speed and Force Tables....
4.3 Derating Tables for Dense Samples
4.4 Chemical Resistance Table:
45 DecontaminationTabe....................................... 12
4.6. ROENoraogra plise serseri! 13
5. Maintenance iii een 14
5.1 Care and Cleaning.…................................................ 14
5.2 Corrosion Cleaning Procedure .
5.3 Storage: Keep the Unit Dry
5.4 Decontamination Procedures
5.5 Ggverlnterleek BYBasS ss Er 14
A 져 15
5.7 Brush Replacement trae 15
58 Calibration........................................... e 15
5.9: Power Cord Inspection snai rit 15
5.10 Condition of Returned Equipment............................. 15
ο ος να ο ο ο ος ο ο ο ο 15
6.Specifications.......................................... 16
ο ο ο μμ eee 16
7A! Warnittg: Message πμ μμ uavescassenes 16
7.2 Error Codes een ené 16
sec asma 16
7.4 DisassembiyforSemice.......................................... 16
O: DraWiNOS ο ο ο ας 18
IM-426 Rev. 10 11 January 2004 Page 3 of 34

1. Introduction
1.1 Product Description
The IEC Centra CL2 is a compact benchtop centrifuge
designed for multipurpose use in medical, industrial, and
scientific laboratories. There are two models:
e Cat. No. 426: 120 V, 50/60 Hz
e Cat. No. 427: 240 V, 50/60 Hz
The IEC Centra CL2 accommodates a wide variety of rotors
including fixed angle and horizontal (swinging bucket). IEC
AeroCarriers™ provide aerosol containment along with
autoclaveability. The centrifuge can handle up to 300 ml
(801 rotor), and reach maximum speeds of up to 8500 rpn:
(841 fixed angle rotor) and 3900 rpm (236 horizontal
rotor).
The system also includes
® a cover interlock for safety and a 'glove-friendly'
membrane control panel with digital speed and time
displays
* an ‘At Speed’ timer mode allowing for accurate
separations
® a continuous 'hold' mode
2. Installation
2.1 Receive the Unit
All units are shipped in protective packaging.
1. Follow the unpacking instructions on the carton.
2. Inspect the unit upon receipt and immediately file any
damage claims with the shipper/carrier.
3. Complete and return the postage-paid warranty card.
2.2 Prepare the installation site
The unit normally resides on a bench top.
1. Place the centrifuge on a clean, dry surface, to make
certain that the suction feet at the bottom grip the
surface firmly. Keep the area beneath the unit free of
debris and loose materials.
/NCAUTION
The resting surface must be level, to ensure quiet,
vibration-free operation. A rigid and stable location
is important. An improperly loaded centrifuge may
vibrate or move
2. Locate the centrifuge to allow a clearance of 8 cm
(3 inches) on each side and 4 in. (10.2 cm) in the rear of
the unit for ventilation.
À WARNING
International Electrotechnical Commission standard
1010 part 2-20 limits the permitted movement of a
laboratory centrifuge to 12” (300 mm) in the unlikely
event of a disruption. Laboratory management
procedures should require that no person or any
hazardous materials enter within this boundary while
the centrifuge operates.
IM-426 Rev. 10 11 January 2004
2.3 Verify Power Configuration
Verify that the correct power cord and connector is
provided for your installation.
1. Using a voltmeter, measure the line voltage to ensure it
is within the limits for your model. For Cat. No. 426 the
line voltage should be between 108 and 132 VAC. For
Cat. No. 427 the line voltage should be between 216
and 264 VAC. Variations in line voltage or frequency
will affect the unit’s speed and acceleration.
2. The unit requires a grounded power supply (3-outlet). If
your facility does not have grounded power outlets,
arrange
for proper grounding.
À WARNING
ELECTRICAL HAZARD!
A Do not remove the grounding pin from the
centrifuge power cord. Do not use the bare
wired power cord to attach a power plug that
does not have a grounding pin.
The power cord provided with the unit is
correctly rated for the highest current demand.
This power cord should not be interchanged
with cords from equipment with lower current
demand. Exchange of power cords between
equipment may create a fire hazard.
2.4 Moving the Unit
To move the unit to a new location:
À WARNING
q The unit can weigh up to 222 Ib. (101 kg).
Use caution when moving to avoid any injury.
1. Check that the new site meets the criteria in Section
2.2 before moving the unit.
2. Before moving, unplug the centrifuge and remove all
accessories and the rotor.
3. Position a flat object, such as a tongue depressor, near
a suction cup at the bottom of the unit.
4. Lift up an edge of the cup, and insert the flat object far
enough to break the vacuum suction seal.
5. When all four suction cups are disengaged, lift the unit
from the work surface.
6. When the unit is in its new location, ensure that the
suction cups adhere correctly to the work surface
Page 5 of 34

3. Operation
3.1 Warnings and Cautions
To Avoid Electric Shock:
Plug the power cord into a grounded outlet.
AWARNING
Never remove the grounding prong from the
A power plug, or use any adapter which does
not complete the grounding circuit.
À WARNING
Always unplug the power cord before
attempting to clean or service the centrifuge.
/NCAUTION
DO NOT exceed maximum rated speed for each rotor/
accessory combination. Maximum speeds can be found
in Section 4.1 Speed and Force Tables. All rotors and
accessories are stamped with their cat. no.for easy
identification.
/NCAUTION
Samples of specific gravity higher than 1.2 require the
maximum speed to be derated.
/NCAUTION
Ensure that loads are properly balanced around the rotor
to minimize vibration. All Thermo Electron accessories
are stamped with their weight for easy balancing.
/NCAUTION
Do not block the vents, otherwise, airflow will be
restricted.
/ACAUTION
Be sure the rotor and accessories are properly installed
before attempting to start a run.
3.2 Opening The Cover
Once the red light over the STOP button is steadily
illuminated (no longer flashing) press the COVER OPEN
button to release the interlock and allow the cover to be
opened.
3.3 Rotor Installation
1. Check that the RED light over the STOP button is ON.
This indicates that power is on, the rotor is stopped and
the cover can be opened.
2. Push the COVER OPEN lever to the right and lift the
cover.
. Lower the rotor straight onto the shaft.
. Screw the knurled metal locking nut (clockwise) onto
the shaft to hold the rotor down (on some rotors, you
must remove any sample tubes first.).
5. Tighten the nut with your fingers; do not use a tool.
NOTE: Rotors with or without a keyway can be used on
the IEC Centra CL2 centrifuge as there is no key on the
shaft.
ро
Page 6 of 34
3.4 Starting and Stopping a Run
To start a run:
1. Use the ARROW buttons to set the desired run time
(O to 30 minutes) in the TIME display, and the desired
rpm in the SPEED display.
2. Press the START button.
The green light under the START button will illuminate,
and the time display will begin counting down. The
actual speed is displayed in the SPEED display. The
centrifuge
will run for the set duration and decelerate to
a stop.
NOTES:
* To terminate a run before time expires, press the
STOP button. T he red light over the STOP button
illuminates when STOP is pressed or time expires, and
it flashes until the rotor comes to a stop.
® The cover may be opened when the rotor speed is
below 20 RPM.
® The time and speed settings cannot be changed during
a run.
* A new run cannot be started until the rotor has come
to a complete stop.
+ For infinite spins (hold mode), use the arrow keys to
scroll up past 30 minutes. The word 'HOLD' appears
in the display. Pressing the START button will begin a
run which can only be terminated by pressing the
STOP button. In the hold mode, the timer counts up.
+ To select the timing mode, use the arrow buttons to
scroll down past O seconds. The letters 'Spd' or 'Acc'
will appear. Press the arrow buttons to toggle between
the two timing modes. 'Spd' is the 'At Speed' timing
mode where the timer starts counting down when rotor
reaches 95% of set speed. Acc' is the normal timing
mode where the timer begins to count down as soon as
the run button is pressed.
3.5 Rotor Removal
1. Remove any sample tubes, shields and other
accessories from the rotor.
2. Unscrew (counterclockwise) approximately one full turn
the knurled locking nut.
3. Place both your thumbs on the knurled locking nut and
grip the rotor with your fingers. Push your thumbs down
and at the same time pull the rotor up with your fingers.
This should dislodge the rotor from the shaft. If
unsuccessful, lightly tap the knurled metal locking nut
with a rubber/plastic mallet or other similar object. The
nut and the rotor can now be removed from the shaft.
IM-426 Rev. 10 11 January 2004

3.6 Balance
A balanced load is essential for all centrifuges. An
unbalanced load produces vibration, and can damage the
unit. ALWAYS balance containers on opposite sides of the
rotor.
NOTE: A 2-gram load imbalance, at a speed of 4600
RPM, imparts force equivalent to 20 pounds (9.1 kg) a
rest. Always ensure that the rotor is loaded
symmetrically, with a full complement of accessories,
and a full (or paired) set of tubes. Tube adapters should
also be installed symmetrically.
Balance load within 1 gram
The rotors are dynamically balanced at the factory. The
manufacturer matches removable parts (trunnion rings,
shields, buckets, and carriers) to within 1 gram, and
stamps the weight on each piece. Check these markings,
whenever you interchange parts, to ensure that opposite
parts are matched. Ensure that the total weight of samples
and removable parts, loaded in opposing positions, are
equal in weight, to within 1 gram. The position numbers,
present on many rotors and adapters, identify opposing
tube positions.
Opposing containers must be alike in shape, thickness, and
distribution of glass or plastic. This is especially important
for large containers.
NOTE:
Swinging Bucket Rotors:
Tubes loaded into swinging bucket rotors must be
symmetric, around the axis of rotation. Verify this by
rotating the entire rotor 180° by hand. The loads
should be in the same apparent positions (not mirror
images). In addition, the loads within each bucket
must, also, be symmetric around the bucket's pivot
axis. Verify this by ensuring that each bucket is loaded
so that it does not tilt from the vertical, when the rotor
is at rest. Maintaining balance within each bucket
ensures that the bucket and the tubes swing out to
horizontal, when the rotor reaches operating speed,
applying centrifugal force toward the bottom of the
tubes. Failure to achieve full swing-out causes vibration
and premature wear of the rotor and the motor.
Samples of like (similar) specific gravities may be
processed in the same run, provided that the samples of
the same type are balanced around the rotor, as though
they were the only pairs in the rotor.
/NCAUTION
Do not exceed maximum rated speed for each rotor/
accessory combination. Maximum rated speeds can be
found in Section 4.2 - Speed And Force Tables.
Rotor Balance
Load
tubes in the following manner:
IM-426 Rev. 10 11 January 2004
1. Load four tubes at
positions: 3, 6, 10,
13
or 2, 5, 9, 12
or 1, 4, 8, 11.
2. Load six tubes at
positions:
6, 7, 3, 13, 14, 10
or 5, 7, 2, 12, 14,
9
or 1, 7, 4, 8, 14,
11
3. Loading an odd
number of tubes is
not recommended.
Avoiding Vibration
All centrifuges have critical speeds at which vibration
occurs. As the speed increases beyond the critical speed,
vibration will cease. This inherent condition also occurs
during deceleration. An imbalanced load intensifies these
critical vibrations. You should avoid running the centrifuge
at or near its “critical speed” to avoid excessive vibration.
Page 7 of 34

4. Applications
4.1 Introduction
This section describes the use of specific rotors and accessories. More detailed information is shipped with the rotor or
accessory itself. This section contains five reference sections:
® Speed and Force Tables
® Derating Table for Dense Samples
e Chemical Resistance Table
* Decontamination Table
* Nomograph
/NCAUTION
Do not exceed maximum rated speed for each rotor/accessory combination. Maximum rated speeds can be found in
Section 4.2 - Speed And Force Tables.
Relative Centrifugal Force (RCF or G-force) at a given speed varies with the rotor, and with the distance away (rotating
radius) from the shaft of the centrifuge (center of rotation). The rotating radius is measured to the furthest inside tip of
the tube, away from the centrifuge shaft. The Speed and Force Tables indicate the maximum speed and RCF that the
centrifuge can achieve with various rotor/accessory combinations. The Derating Table specifies reductions in maximum
RPM, when spinning samples with specific gravity above 1.2.
Use of any tube above its rated RCF can cause tube cracking. To avoid this, compare the G forces, specified in the Speed
and Force Tables, with the ratings for the tubes that you are using. If the tubes are not rated for the force that the
centrifuge will apply, look up their reduced g force rating and enter it on the control panel.
Corrosive Solvents
Your centrifuge is made of materials designed to resist immediate attack from most laboratory chemicals. Prolonged
exposure should be avoided, by immediately removing the chemical from rotor or assembly. Rotors and accessories
placed in the chamber are made of a variety of materials, including aluminum and polypropylene. The Chemical Resistance
Table shows the suitability of each material with different classes of reagents.
Section 5.2 describes how to clean and remove corrosion from the chamber, rotors, and accessories. Follow these
instructions and clean spills promptly to minimize the effect of corrosive chemicals and to avoid expensive repairs.
Page 8 of 34 IM-426 Rev. 10 11 January 2004

4.2 Speed and Force Tables
Rotor 215 4-Place
Swinging Bucket Rotor
Tube Maximum = i Shield or =
No. x Vol. (ml) Description RPM / RCF Radilis Tr Ring Carrier Adapter Cushion
8x50ml Falcon/Corning conical plastic - 8х315
Corning 8300-50 conical glass 3100 1510 14.1 4x326 8x320 E 571
8x15ml Falcon/Corning conical plastic 8x1106 570
4x50ml Falcon/Corning conical plastic - 4x315
Corning 8300-50 conical glass 3350 1750 13.9 4x325 4x320 - 571
4x15ml Falcon/Corning conical plastic 4x1106 570
Falcon/Corning conical plastic 3175 1750 15.4 4x350 4x323 - 315
4x50ml sealed - - -
Falcon/Corning conical plastic 3350 1750 13.9 4x325 4x320 4x1106 4x668
4x15ml sealed Falcon/Corning 1106 4x571
4x10-15ml sealed Vacutainer 16x100-125mm 3175 1710 15.2 4x350 4x7323 4x668
4x7ml sealed Vacutainer 13x100 4x1105 4x571
12x10ml 16x100mm
34 č 4x1013 0 12x7mi 16x75mm 50 1725 12.9 x10 57
ты mm 3450 1700 12.8 4x366 | 4x1018 E
16x5ml 13x75mm 567
20x5ml 12x75mm
40 1 b. 4x369
20x3ml 10x75mm 00 1975 na *
Rotor 221 6-Place Fixed Trunnion Swinging Bucket
Tube Maximum >. i Shield or i
No. x Vol. (ml) Description RPM / RCF Radius ile King Carrier Adapter Cushion
6x15ml Falcon/Corning
6x12.5ml Kimble 45170-125 OR ЕВ io fixed ως = 668
6x10mi Corning 8080-10 3500 1890 13.8 6x356 669
Rotor 236 4-Place Aerocarrier Horizontal Swing-out Rotor
Tube Maximum = E Aero E
No. x Vol. (ml) Description RPM / RCF pe Rio Carrier Adapter, ος.
4x50ml . 3400 1950 15:0 4x2091S
8x1 5mi Falcon/Corning
8x10ml Kova/Urisystem ο. is ní
16x10ml Vacutainer 16x100mm
16x7ml Vacutainer 13x100mm 3700 2200 14.3 fixed 4x2093S =
Hemogard Vacutainer 13x100mm
28x7ml Vacutainer 16x75mm
28x5ml Vacutainer 13x75mm 3900 2150 12.7 4x2094S
Hemogard Vacutainer 13x75mm
Rotor 801 6-Place 45 degree Fixed Angle Rotor
Tube Maximum
Radi Shield Adapt Cushi
No. x Vol. (ml) Description RPM / RCF LS le aptos LEON
6x50ml Falcon/Corning conical plastic = 6x315
Corning 8300-50 conical glass 3900 2050 12.1 6x305 = 571
6x15ml Falcon/Corning conical plastic 6x1106 570
6x50ml Falcon/Corning conical plastic = 6x315
Corning 8300-50 conical glass 4500 2450 10.8 6x320 = 571
6x15ml Falcon/Corning conical plastic 6x1106 570
Rotor 804S 4-Place 40 degree Fixed Angle Rotor
Tube
Maximum
E 3 :
No. x Vol. (mi) Description RPM / RcF | Pedus | Shield | Adapter | Cushion
4x50ml Falcon/Corning conical plastic - 315
Corning 8300-50 conical glass 4200 2270 11.5 323 - 571
4x15ml Falcon/Corning conical plastic 6x1106 570
4x60ml Corning 8540-60 2500 1000 14.3 4x341 = 572
IM-426 Rev. 10 11 January 2004 Page 9 of 34

Rotor 809 12-Place 45 degree Fixed Angle Rotor
Tube Maximum = x >
No. x Vol. (ml) Description RPM / RCF | Radius | Shield | Adapter | Cushion
12x15ml Falcon/Corning conical plastic 3800 2150 13.3 668
Corning
8080-15 conical glass 3800 2050 12.7 302 570
126xDevice Amicron Filtration Device 3900 2050 13.4 -
12x10ml Corning 8080-10 4500 2310 10.2 12x356 > 570
12xDevice Filtron or Millipore Devices 4500 2490 11.0 =
Corning 8080-10 4100 2270 12.1 12x668
vagoni 17x102mm 4100 2120 11.3 dea 570
Rotor 841 12-Place 45 degree Fixed Angle Rotor
Tube Maximum Radius User
No. x Vol. (ml) Description RPM / RCF Adapter
12x1.5-2.0ml 8500 4680 5.8 =
12x0.7ml 8500 4770 5.9 5763
12x0.5ml microtubes 8500 3960 4.9
12x0.4ml 8500 4680 5.8 5764
12x0.25ml 8500 3630 4.5
4.3 Derating Tables for Dense Samples
The Speed and Force Tables list the maximum speed for each rotor/adapter combination for the IEC Centra CL2 Series
Centrifuges. These speeds are specified for samples whose specific gravity is not greater than
* 1.2 for swinging bucket rotors
* 1.5 for angle rotors
For denser samples, the maximum specified speed in RPM is reduced (derated) by a factor from the table below:
Derating Factor for:
Specific Gravity Swinging Bucket Fixed Angle
1.2 1.000
1.3 -960 1
1.4 .925
1.5. „894
1.6 «866 „967
+7 „839 939
1.8 816 912
1.9 .794 .888
2.0 .774 .866
2.1 .755 -844
2.2 .738 .825
2.3 „721 «807
2.4 „707 .790
2.5 .692 .774
2.6 .678 .758
2.7 .666 .744
2.8 .654 -731
2.9 .642 719
3.0 .632 „707
Derating Example: A rotor rated for 4,000 RPM used with samples with a specific gravity of 1.4, cannot spin faster than
3,700 RPM. (4,000 x .925 = 3,700)
Specific gravities greater than 3.0:
This
table is based on the formula:
v(s,/S,)
Use the same formula to compute derating factors for specific gravities greater than 3.0.
® So is the maximum specific gravity allowed before derating (1.2).
® S, is the actual specific gravity of the sample.
/NCAUTION
Do not exceed the rated speed or specific gravity. Higher speeds or specific gravities will impose unnecessary wear on
the centrifuge and can cause rotor failure. Wear and damage caused in this manner are not covered under warranty.
Page 10 of 34 IM-426 Rev. 10 11 January 2004

4.4 Chemical Resistance Table
The centrifuge, rotors and accessories are comprised of made of materials that are designed to resist attack from most
laboratory chemicals. For your convenience, the chemical resistance table below shows the suitability of various materials
with different classes of reagents.
NOTE: Refer to the cleaning section for information on cleaning and removing corrosion from various parts. Clean spills
promptly to minimize the effect of corrosive chemicals and avoid expensive repairs.
Plastic Metal Other
3N37AHLIATOd
q
ヨ
ldIdOW
(@NIYT30)
YINATOdONOH
171397
(@NO9739)
HINATOdOI
TVLIIV
3NIHALSA1Od
WNINVLIL
INNNINNIV
卫
x
m
=
で
ов
레더
E
π]ο
m|x
11%
г
三 | 三
Ko)
고
<
É
©
YINOTIVATOd
ALVNOSYVOA1Od
ヨ
N
ヨ
AdOHd
人
1Qd
] ヨ
ヨ
1S
SS
ヨ
1NIV1S
3ZNOY8
ISINVOVIN
WNISINDVW
43914
2I1ON
ヨ
Hd
e or weal
cids*, strong or conc.
p
ses
sters
5.
rocar , aromatic
‘oca , halogenat
etones
ng Agents, strong
*For Oxidizing Acids, see "Oxidizing Agents, strong”.
Classification
of Resistance
= EXCELLENT
G = GOOD
F = FAIR
N = NOT RECOMMENDED
IM-426 Rev.10 11 January 2004 Page 11 of 34

4.5 Decontamination Table
Compatible Processes For Decontamination
Sterilization Methods Plastic Metal Other
r >
3 2 | 2 ñ 8 S 5 で a
в ЗЕ ВЕ | 2
s$| 8 | 3 | 人 | 68595 z | 5 F sl o 2| 8 <| 6 2
c| 5 Y|8| Dlog 228 É 2 2 | D | E の | = o 2 |= 드 m
5/8) 2] 3) 5/22 28/83] 29/5) 2/212) Ba] Ble) se} se] 2
2 > E ni モエ | 己 避 28 m = o < S| < 고 z n e
m|S|
2
οι
σε
2
al
8 э
=
Pla
D 3 m = м |= に = δι m 그 5 m
πι 9 = A E N 5
고
m
고
m
Е D
Mechanical
Autoclave* 5 Μ υ 5 Μ υ S $ $ U S S 9 S $ 5 $ м $ м
Ethylene Oxide Gas 5 S $ $ $ $. 5 5 5 $ 5 5 95 S $ U U 5 5 $
Dry Heat (2Hrs. @ 160°C) U U U U U U U U υ υ S S U 5 S U U U U U
Chemical
Ethanol $ S $ 5 υ $ 5 S U M 5 $ S 5 5 5 $ 5 S 5
40% Formalin 5 5 5 $ U S S S S U S S S S S S U S S S
Methanol S M S S M S S S U M S S S S S S S U S S
2-Propanol S S S S M S S S U S S S S S M S S 5 5 5
-5% Sodium Hypochlorite* * $ $ 5 5 U $ U U υ $ 5 м U U U 5 υ 5 5 м
3% Hydrogen Peroxide 5 E S Ss 5 S M S U $ E 5 5 5 U 5 5 5 5 M
100% Hydrogen Peroxide $ s 5 S 5 υ υ υ υ 5 5 5 5 $ 5 υ υ 5 5 υ
5% Phenol Solution M U U S U υ Μ Μ υ Μ Μ Μ Μ Μ Μ Μ υ 5 S U
*For Oxidizing Acids, see "Oxidizing Agents, strong”
À WARNING
Autoclaving 121° C for 20 min. @ 2 ATM (15 PSIG)
** 1 to 10 Dilution of Household Bleach
S = SATISFACTORY
M - MARGINAL
U - UNSATISFACTORY
This chart describes the material compatibility of various sterilization methods. It does not specify the adequacy of
sterilization. Refer to the Chemical Resistance Table on the previous page for material compatibility during
centrifugation.
IM-426 Rev. 10 11 January 2004 Page 12 of 34

4.6 RCF Nomograph
Rotating
Radius
(centimeters)
Ta,
+ 8
3 一 上
十 -7
A 6
2— 5
IM-426 Rev. 10 11 January 2004
Using the RCF Nomograph
n
Relative
Centrifugal
Force
(gravities)
To determine the relative centrifugal .
force (ref), place a straightedge on the È
nomograph connecting the known speed
(rpm) and the known rotating radius. The
point at which the straightedge intersects
the ref axis is the force.
For example, if the rotating radius is 10 cm
and the speed is 3,000 rpm, the relative
centrifugal force is 1,000 xg (gravity).
If the force and the radius are known, the
corresponding speed can be determined.
To Calculate RCF
АСЕ = .00001118 хгх №
АСЕ = relative centrifugal force (gravities)
r = rotating radius (centimeters)
N = rotating speed (rev. per min. or rpm)
30,000
20,000
10,000
5,000
№ © © o
100
50
20
10
TT
|
T
| T
JM
還
II
に テー
T
TT
|
oi
emip
20,000
15,000
10,000
6,000
4,000
3,000
2,000
1,000
500
200
Page 13 of 34
Speed-
Revolutions
per
minute

5. Maintenance
5.1 Care and Cleaning
® Keep the centrifuge clean to ensure good operation anc
to extend its life.
+ Clean the sample chamber, rotor, and lid, at the end of
each work day, and immediately after any spill. Use a
damp sponge, warm water, and a mild liquid detergent
suitable for washing dishes by hand, such as Ivory“
liquid. Do not use caustic detergents or detergents tha:
contain chlorine ions. These attack metals.
Remove stubborn stains with a plastic scrub pad. Do
not use steel wool, wire brushes, abrasives, or
sandpaper as they create corrosion sites. Never pour
water directly into the rotor chamber.
Scrub the rotor’s tube cavities with a stiff test tube
brush that has end bristles and a non-metallic tip. Afte’
cleaning, dry each part with a clean absorbent towel.
/NCAUTION
In the case of glass breakage, be careful to remove
ALL particles of glass from the unit! In particular, be
sure thoroughly scrub adapters and cushions with a
wire brush or replace these items as glass shards
embedded in the adapters can cause further
breakage.
5.2 Corrosion Cleaning Procedure
The rotors and structural accessories are finished to give
maximum resistance to corrosion. To maximize the life of
the unit, continually inspect the rotor cavities for corrosion
especially if you use chloride ion solutions such as sodium
chloride (saline), and sodium hypochlorite (household
bleach), because these solutions attack most metals.
Clean the rotor, rotor chamber, and accessories
(particularly the sample compartments and bucket cups)
thoroughly after each exposure. Inspect all surfaces unde
bright light for corrosion. Be aware that small crevices
grow deeper, eventually resulting in system failure.
If you see any corrosion, remove it immediately, using the
following procedure:
1. Follow the cleaning procedure at the start of this
section.
2. Soak the product in mild hand dish-washing detergent
and scrub the product thoroughly with a stiff test tube
brush. The brush should have end bristles and a non-
metallic tip.
3. Soak the product in clear warm water for a minimum of
an hour.
4. Rinse the product in warm water then in distilled water.
5. Dry the product thoroughly with a clean absorbent
cloth.
/NCAUTION
If this procedure does not remove the corrosion,
discontinue use of the product and inform technical
service.
Page 14 of 34
5.3 Storage: Keep the Unit Dry
Store parts on a soft surface to avoid damage.
Rotors and other parts should be clean and dry. Store them
open to the air, not in a plastic bag, so any residual
moisture evaporates. Face the parts upward to avoid
moisture retention in the cavities.
5.4 Decontamination Procedures
AWARNING
If tube breakage occurs releasing toxic, infectious,
pathogenic, or radioactive material into the unit,
decontaminate the chamber.
Rotors have sealed containers that provide aerosol
containment and, if used as directed, keep spillage
confined. If breakage occurs it may be sufficient to only
decontaminate the sealed carriers.
The Decontamination Table in the previous section lists the
sensitivity of various materials to common sterilization
procedures. When using a 1-to-10 dilution of household
bleach (sodium hypochlorite) to decontaminate metal rotors
or accessories, follow decontamination by the corrosion
cleaning procedure (5.2) since chloride ions attack most
metals.
Always decontaminate for the minimum recommended
time. If you observe corrosion, remove it as described
earlier, discontinue use of the method and use an alternate
decontamination procedure.
Polypropylene sealed carriers can be autoclaved. Remove
any sample tubes before autoclaving unless they are
completely full of sample. Remove caps, stoppers, and
other tube closures before autoclaving to keep the tubes
from collapsing under pressure. Autoclave the rotor and
accessories at 121° C @ 15 psig for 20 minutes. Do not
stack polypropylene rotors during this process. After
cooling, perform a normal cleaning operation as described
above.
Repeated autoclaving seriously degrades the performance
of polycarbonate sealing covers.
5.5 Cover Interlock Bypass
The cover will remain locked if power fails. If you need to
remove samples from the unit before power is restored, use
the cover interlock bypass after the rotor has come to a
stop.To bypass the cover interlock:
1. Unplug the centrifuge.
2. Locate the hidden plastic plug underneath the front
ledge of the cabinet.
3. Use a screwdriver to pry out and remove the plug.
Pull the attached cord to release the cover interlock.
. Replace the plug in the hole.
À WARNING
Do not perform this operation routinely. The
centrifuge’s cover interlock provides operator safety. It
allows the cover to be opened promptly, whenever
rotation has stopped.
sa
IM-426 Rev. 10 11 January 2004

5.6 Fuses
Fuses are located internally within the centrifuge. They
should only be replaced by qualified service personnel.
1. Remove all rotor and accessories from the chamber.
2. Gently tilt the unit onto its side and remove the four he»:
head screws which secure the baseplate.
3. Unscrew and remove the four rubber feet and lift the
baseplate off.
4. The fuse(s) is mounted to the cabinet housing. Replace:
fuse(s) with:
For 100/120 VAC 1 -4A, .25x1.25 in.part no. 40340
For 220/240 VAC 2 - 2A, .25x1.25 in.part no. 40794
5.7 Brush Replacement
lt is important to check the brushes periodically since
damage to the motor can occur if the brush is allowed to
wear down to the spring.
À WARNING
DISCONNECT THE POWER CORD BEFORE REMOVING
THE BRUSHES.
1. Remove all rotor and accessories from the chamber.
2. Gently tilt the unit onto its side and remove the four
screws which secure the baseplate. The brush caps are
located on either side of the motor housing. Unscrew
each cap with your fingers (or use a small flat
screwdriver) and remove the brushes. There are two
black caps which can be removed from the housing to
allow use of a large screwdriver.
3. Measure the length of the brushes and replace both
brushes if either one is less than 1/4 of an inch long.
4. Reinstall all parts removed.
/NCAUTION
WHEN REINSTALLING INSPECTED BRUSHES
When brush replacement is not required it is
important that a brush be inserted in the same
position as it was removed. The trailing edge of the
brush must be positioned properly. The trailing edge
may be identified by the presence of a dark deposit
of carbon along that side.
NOTE: New brushes may require a burn-in period of up
to a half hour.
5.8 Calibration
The speed sensor used in the IEC Centra CL2 requires no
calibration however you should verify its speed once every
24 months. This can be done by using an optical
tachometer through the clear plastic viewport in the lid.
Notify technical service if this measurement indicates
instrument failure
5.9 Power Cord Inspection
Inspect the power cord every four months for signs of
wear. Refer servicing to qualified personnel only. Replace
power cord with Thermo Electron part number 44392 only.
IM-426 Rev. 10 11 January 2004
5.10 Condition of Returned Equipment
Obtain a return goods authorization (RGA) before returning
equipment to the manufacturer. The RGA paperwork
includes a Certificate of Decontamination for you to sign.
It indicates that you have performed the proper steps for
decontaminating the unit.
À WARNING
All returned units must be decontaminated, free of
radioactivity, and free of hazardous, infectious,
pathogenic, or toxic materials.
All return equipment shipments will be refused until the
signed certificate is received.
You must prepay transportation to the service depot.
5.11 Warranty
Warranty information is provided on the warranty card
supplied with the centrifuge.
Page 15 of 34

6. Specifications
8,500 RPM (Angle Rotor No. 841)
RetationiSpasd 3,900 RPM (Horizontal Rotor No. 236)
4775 xg (Angle Rotor No. 841)
Maximum RER 2200 xg (Horizontal Rotor No. 236)
Maximum Capacity: 400 mL (8x50 mL)
Sound Level 65 dB(A)
Timer
O to 30 minutes
0 to 1 minute by 5 seconds
1 to 5 minutes by 15 seconds
5 to 30 minutes by 1 minute
HOLD mode up to 99 min., 99 sec.
Range Increments
Accuracy +1.0 %
Speed
Range 1000 to 8500 rpm by 100 RPM
Accuracy +100 RPM
Power
120 VAC + 10%, 60 Hz (Cat. No. 426)
生
Power Requirement’ | 240 VAC + 10%, 60 Hz (Cat. No. 427)
Heater Output
a 175 watts (600 Btu/hr.)
(typical)
Dimensions
Cover Closed: 11 in. (28 cm)
Height Cover Opened: 24 in. (61 em)
Width 13 in. (34 cm)
Depth 16 in. (40 cm)
Unit Weight 24 Ib. (11 kg)
Shipping Weight 29 Ib. (13 kg)
Specifications subject to change without notice.
7. Service
7.1 Warning Messages
A Warning Message indicates improper operation and may
be cleared by opening the lid and correcting the problem.
Display Meaning
The cover was not properly closed when
was opened during a run.
Lid the start button was pressed, or the cover
PFL
run.
Power to the centrifuge was lost during a
7.2 Error Codes
Error Codes indicate a malfunction of the centrifuge. They
are cleared by disconnecting and reconnecting power to
the centrifuge. If an Error Code or Warning Message
persist, service may be reguired.
Display Meaning
Tachometer signal not present during a
run.
Er1
decelerating for more
than 2 seconds, or speed is over 9000
RPM at any time.
Er2
Speed is 500 RPM over set speed and not
Er 10 the maximum limit
(94 volts).
The motor voltage was detected to be over
Page 16 of 34
7.3 Troubleshooting
Centrifuge won't start:
1. If the rotor stopped indicator (red LED) is lit, there is
power to the centrifuge. If it is not lit, unplug the
centrifuge and check the fuse(s).
2. Spin the rotor by hand to see that the “rotor stopped
indicator” begins flashing, and listen for the latch to
engage. This will verify the function of the tachometer
and latch.
7.4 Disassembly for Service
ΑΝΝΑ ING
SHOCK HAZARD.
The unit uses AC power, and some of the
service procedures require operation with
panels removed, exposing power lines. This
introduces the risk of electric shocks. Service
should
be performed by qualified personnel
only. Do not touch exposed wires without
first unplugging the unit.
ZKCAUTION
STATIC ELECTRICITY DAMAGE.
The circuit boards contain electronics that can
be damaged by static electricity. Persons
doing extensive maintenance on the circuit
boards, or removing individual components
from the circuit boards, should be grounded
(such as by wearing a wrist strap.) When
shipping a circuit board, always enclose it in a
static-protective bag.
1. Unplug the centrifuge from its power source.
2. Remove the rotor and accessories from the chamber.
3. Gently tilt the unit onto its side and remove the four hex
head screws which secure the baseplate.
4. Unscrew and remove the four rubber feet and lift the
baseplate off.
5. With the cover open, remove the four screws (two on
each side) from the control tower. Gently pull the tower
outward without removing it completely (there are wires
connecting it to the chamber).
6. To remove the guard bowl, locate and remove the 9
screws at the base of the centrifuge chamber. To
remove the cover from the guard bowl, pry off one end
cap from the hinge pin. Slide the pin out.
Interlock
The centrifuge has a safety interlock which prevents the
cover from being opened unless the rotor is turning 20 RPM
or less. There is a switch in the interlock assembly which
senses that the cover is closed and locked. It is a normally
open switch and closes when the solenoid is in the locked
position. When pressed, the STOP/COVER OPEN button
releases the safety interlock by powering the solenoid
through the Interlock PC board.
® The solenoid coil has a resistance of approximately 80
Q. Power to the solenoid should be approximately 170
VDC.
Voltage is provided by the Interlock PCB when
the STOP/COVER OPEN button is pressed.
IM-426 Rey. 10 11 January 2004

e To measure the voltage, disconnect the solenoid at JP4
and read across the BRN and YEL leads. Verify the
function of the switch using an ohmmeter.
e To replace the interlock, remove the four screws which
secure the latch assembly to the cabinet. Snip the wire
ties in order to disconnect all leads to the latch
assembly. Make note of wiring before disconnecting.
Reconnect wires as noted, or using the diagram
provided at the end of this manual.
The slots in the latch assembly mounting bracket are for
proper positioning. Raise or lower the height of the
assembly so that when the cover is closed completely
switch SW1 (WHT and WHT/BLK leads) is engaged by the
roll pin of the solenoid plunger. A run may not be started
until the switch is engaged.
Timer PCB
The IEC Centra CL2 has a Timer/Display PCB mounted
behind the membrane control panel. The timer PCB
contains the EPROM and delivers power to the motor. To
replace it, the tower must first be removed (see See 7.4
“Disassembly for Service” on page 16). The PCB is
secured to the tower by four screws
/ACAUTION
STATIC DAMAGE.
To prevent damage to the PC board, always use a static
protective device (such as wriststrap) when handling or
servicing.
Motor
The IEC Centra CL2 uses an AC series wound drive motor.
The motor is not a singular replaceable component, as the
base housing of the centrifuge serves as the motor
housing. The motor is comprised of an armature (includes
bearings), field, brushes, brush holders, brush caps, brush
leads, and a magnetic rotor. All of these parts are available
separately (as well as motor bearings) for repair purposes.
® When isolated, the armature resistance should be
approximately 6.3 Q. This can be measured by
disconnecting the RED and WHT motor leads and
measuring the resistance across them. When isolated,
the field resistance should be approximately 10 Q. It
can be measured by disconnecting the Red and BLK
motor leads (BLK at BR1).
* To access the motor, remove the baseplate (See 7.4
“Disassembly for Service” on page 16) and then the top
cap located in the guard bowl. Two Phillips head
screws secure the top cap. When removing the top
cap, take care not to lose the pre-load washer which
rests on top of the upper bearing.
e To replace the armature, the brushes must be removed
(See 5.7 “Brush Replacement” on page 15), and the
magnetic rotor taken off of the motor shaft. Magnetic
rotor removal requires that the Interlock PCB be
removed (see instructions above). It is secured with
Loctite® 454. Use care not to break the magnetic rotor.
Once brushes and the magnetic rotor have been
removed, the armature simply lifts out of the housing.
The field rests inside the housing. To remove it, simply
disconnect the three motor leads (RED, WHT, BLK) and
lift it out.
Located below the field are the two set screws which
secure the brush holders in place. To replace or adjust a
IM-426 Rev. 10 11 January 2004
brush holder, loosen the set screw. This allows the
brush holder to be moved or removed. The set screw
may be accessed through the two holes in the field
through which the top cap mounting screws secure the
top cap to the base.
The Interlock PCB in the IEC Centra CL2 disables power to
the interlock during rotation. This PC board is mounted
below the motor. To sense rotation, it uses a Hall effect
sensor to pickup pulses form a magnetic disk mounted on
the motor shaft.
ZAXCAUTION
STATIC DAMAGE.
To prevent damage to the PC board, always use a static
protective device (such as wriststrap) when handling or
servicing.
To replace the PCB, disconnect the wiring harness and
remove the four mounting screws which secure the PCB to
the base housing. Take care not to damage the speed
sensor when lifting the PCB off of the housing.
Page 17 of 34

8. Drawings
Drawing
No.
Description
426
CL2
Assembly
and
Parts
List
10944
Wiring
Diagram
10895
Timer/Display PCB
Schematic
44416
Timer/Display
PCB
Layout
PL-44416
Timer/Display
PCB
Parts
List
10875
Interlock
PCB
Schematic
44566
Interlock
PCB
Layout
44566
Parts
list
Interlock
PCB
Parts
List
Page 18 of 34 IM-426 Rev. 10 11 January 2004

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Page 220134