Thermo FINNPIPETTE DIGITAL User manual

Labsystems
1 2 0
FINNPIPETTE
DIGITAL
INSTRUCTIONS FOR USE
OPERATING
BUTTON
TIP
EJECTOR
PUSHER
TIP CONE
TIP EJECTOR
HANDLE
DIGITAL
DISPLAY
GRIPPY
Thermo Electron Oy
P.O.Box 100, Fin-01621 Vantaa, Finland
Tel. [phone], fax -358-9-3291 0414
www.thermo.com/finnpipette

-2-
PIPETTE DESCRIPTION
The FINNPIPETTE DIGITAL is a
continuously adjustable, general
purpose micropipette for sampling and
dispensing accurate amounts of liquids.
It operates on air displacement principle
(= air interface) and uses detachable,
disposable tips.
The adjusted delivery volume is
displayed in clear digits on a readout
window in the handle.
FINNPIPETTE DIGITAL pipettes cover a
volume range of 0,5-10 ml.
Code Volume Scale
range division
4027000 0,5 - 10 µl 0,1 µl
4027250 2 -20 µl 0,1 µl
4027010 5 - 40 µl 0,5 µl
4027260 20-200 µl 1,0 µl
4027020 40 - 200 µl 1,0 µl
4027030 200 - 1000 µl 5,0 µl
4027040 1 - 5 ml 50 µl
4027050 2-10 ml 100µl
All models are equipped with a built-in
tip ejector.
RAW MATERIALS
The FINNPIPETTE DIGITAL is made of
mechanically durable and chemically
resistant materials.
DIGITAL DISPLAY (Fig.1)
The delivery volume of the pipette is
displayed on read-out window in the
handle.
DESCRIPTION OF TIPS (Page16)
Finntips are recommended for use with
the FINNPIPETTE DIGITAL.
They are made of natural coloured
polypropylene, generally regarded as
the only contamination free material
suitable for tips.
Finntips are also autoclavable (121°C).

-3-
PIPETTE OPERATION
SETTING THE DELIVERY VOLUME
(Fig.2)
To increase the delivery volume turn the
operating button counterclockwise and
to decrease turn clockwise (operating
button facing you) .
Make sure the desired delivery volume
clicks into place and the digits are
completely visible.
Caution: Accuracy and precision are
valid only within the nominal volume
range.
Do not set volumes outside the range.
Using excessive force to turn the
operating button outside the range may
jam the mechanism and eventually
cause defects.
TIP EJECTION (Fig.3)
Direct the pipette towards a suitable
waste receptacle.
Press the tip ejector down and the tip
will be cleanly ejected.
PIPETTE TECHNIQUES
FIGURES 4-7:
A = READY POSITION
B = FIRST STOP
C = SECOND STOP
The operation is controlled by the
operating button.
For best possible accuracy:
Operate the operating button slowly
and with steady speed at all times,
especially when working with liquids of
high viscosity.
Never let the operating button snap back.
Make sure the Finntip is firmly attached
to the tip cone of the pipette and that
there are no foreign bodies inside the tip
itself.
Wet a newly attached tip with the
solution being pipetted before any
actual pipetting takes place.
This is done by filling and emptying the
tip 2-3 times.
Hold the pipette vertically during intake
of the solution.
The temperature of the tip and pipette
should be equalized to that of the
solution.
For maximum hand comfort hold the
pipette lightly in your hand with the
grippy resting over your index finger.

-4-
FORWARD TECHNIQUE ( Fig.4)
1. Depress the operating button to the
first stop.
2. Immerse the tip slightly about 1 cm
below the surface of the solution and
release the operating button slowly.
The tip has now been filled.
Withdraw the tip from the solution. Wipe
any drops from the outside of the tip
without touching the orifice.
3. Deliver the liquid by gently depressing
the operating button to the first stop.
Then after a delay of about a second
continue to depress the operating button
all the way down to the second stop.
The tip has now been emptied. Drop
formation is avoided by touching the
side of the vessel with the tip. Withdraw
the tip.
4. Release the operating button to the
ready position.
If necessary change the tip and
continue with your pipetting.
REVERSE TECHNIQUE (Fig.5)
The reverse technique is suitable for
pipetting solutions with high viscosity
and/or solutions that tend to foam easily.
This technique is also recommended for
pipetting very small volumes.
1. Depress the operating button all the
way to the second stop.
2. Immerse the tip slightly about 1 cm
below the surface of the solution and
release the push button slowly.
The tip has now been filled.
Withdraw the tip from the solution with
the tip sliding along the vessel wall.
3. Deliver the liquid by gently depressing
the operating button to the first stop.
The tip has now been emptied. Drop
formation is avoided by touching the
side of the vessel with the tip.
Withdraw the tip.
Some liquid will remain inside the tip and
should not be included in the delivery.
4. The remaining liquid is either
discarded with the tip or pipetted back
into its original container.
Please note: if bigger volumes than 4 ml
are pipetted with reverse technique
there is no room in the tip for the extra
remaining liquid. Normally reverse
technique is not used for big volumes
like this.
However bigger volumes than 4 ml of
foaming liquid can easily be pipetted as
follows: Use forward technique steps 1
and 2 to fill the tip.

-5-
Deliver by gently depressing the
operating button to the first stop. Drops
outside the tip should be included in the
delivery.
REPETITIVE TECHNIQUE (Fig.6)
The repetitive technique offers a rapid
and simple procedure for repeated
deliveries of the same liquid with the
same volume.
1. Depress the operating button all the
way down to the second stop.
2. Immense the tip slightly about 1 cm
below the surface of the solution and
release the push button slowly.
The tip has now been filled.
Withdraw the tip from the solution with
the tip sliding along the vessel wall to
avoid drops.
3. Deliver the liquid by gently depressing
the operating button to the first stop.
The desired volume has no been
delivered.
Hold the operating button at the first
stop.
Some liquid will remain inside the tips
and should not be included in the
delivery. Drops outside the hp should be
included in the delivery.
4. Immense the tip slightly below the
surface of the solution and release the
operating button slowly
The tip has been refilled.
5. Continue repeating procedures 3 and 4.
PIPETTING WHOLE BLOOD (Fig.7)
e.g. deproteinization in blood glucose
determination)
Use forward technique procedures I
and 2 to fill the tip with blood.
Wipe the tip carefully with a dry and
clean tissue.
1. Immerse the tip into the reagent and
depress the operating button to the first
stop, making sure the tip is well below
the surface.
2. Release the operating button slowly
to the ready position.
The tip has now been filled with
reagent.
Do not lift the hp out of the solution.
3. Depress the operating button the first
stop and release slowly.
Keep repeating this procedure until the
interior wall of the tip is clear.
4. Finally, depress the operating button
all the way to the second stop to
completely empty the tip.

-6-
MAINTENANCE
When the Finnpipette Digital is not in use
make sure it is safely stored in a vertical
position.
SHORT TERM CHECKING
The pipette should be checked at the
beginning of each day for dust and dirt
on the outside surfaces of the pipette.
Particular attention should be paid to the
tip cone of the pipette.
Solvents should not be used for
cleaning the pipette.
LONG TERM MAINTENANCE
(0,5-5 ml) (Fig.8)
The piston and the cylinder should be
checked at least twice a year if the
pipette is in daily use.
Opening the pipette
1. Depress the tip ejector pusher.
2. Insert the tooth of the opener into the
opening at the base of the tip ejector
pusher.
3. Pull away the tip ejector shaft and tip
ejector pusher.
4. Remove the tip cone by turning it in a
counterclockwise direction with the
service tool provided in the package.
5. Pull out the piston.
6. Remove the O-ring from the tip cone.
In model 5-40 µl the two O-rings ate
located deep inside the tip cone.
They can be removed by using the thin
end of the piston.
7. Clean the piston, the piston spring
and the O-ring with a dry, napless cloth.
8. Check the cylinder for foreign particles.
Please note that the cylinder must not be
greased.
9. Grease the cleaned parts with high
vacuum stop cock lubricant.
10. Reassemble the parts.
LONG TERM MAINTENANCE
(10ml) (Fig.9-10)
If the pipette is used daily it should be
checked at least twice a year. The
servicing procedure starts with
disassembly of the pipet.
1. First remove the Tip Ejector pusher by
pushing it all the way down and lifting
the base with a screwdriver blade or
with the tooth of the Service Tool to
release the snap joint.
2. Next remove the Tip Ejector part 2
from part 1 using the maintenance pliers
to release the snap joint.

-7-
3. Remove the Cylinder by pressing the
tip ejector part 1 firmly towards the
cylinder. This action releases the snap
joint and you can pull the cylinder away.
4. Clean the O-ring and cylinder.
Regrease the O-ring and the cylinder.
5. Assemble the parts in the opposite
order to removal. All joints are a snap fit
and are made simply by pushing the
parts together by hand. Be careful not
to bend the pipette during assembly
because this could damage the snap
joints or the piston.
CALIBRATION
All Finnpipettes are factory calibrated
and adjusted to give the volumes as
specified with distilled or deionized
water. Normally, the pipettes do not
need adjustment, but they are
constructed to permit recalibration and
adjustment for liquids of different
temperature and viscosity.
DEVICE REQUIREMENTS AND
TEST CONDITIONS
An analytical balance must be used.
The scale graduation value of the
balance should be chosen according to
the selected test volume of the pipette:
Volume range readable graduation
under 10 µl 0.001 mg
10-100 µl 0.01 mg
above 100 µl 0.1 mg
Test liquid: Water, distilled or deionized,
“grade 3” water conforming ISO 3696.
Tests are done in a draft-free room at a
constant (±0.5°C) temperature of
water, pipette and air between 20°C to
25°C.
The relative humidity must be above
55%. Especially with volumes under
50µl the air humidity should be as high
as possible to reduce the effect of
evaporation loss. Special accessories,
such as the evaporation trap, are
recommended.
CAUTION!
The Finnpipette is designed to allow easy in-lab
service. If you, however, want to send the
pipette to us or to our local representative for
service, please enclose a list of any
infectious, radioactive or otherwise hazardous
materials that have been pipetted. Also please
note that the postal authorities in your country
may limit the sending of contaminated
material by mail.

-8-
CHECKING THE CALIBRATION
The pipette is checked with the
maximum volume (nominal volume) and
with the minimum volume or 10% of
maximum volume, whichever is higher.
E.g. Finnpipette 0.5-10 µl is tested at
10µ and 1 µ. A new tip is first pre-
wetted 3-5 times and a series of ten
pipettings are done with both volumes.
A pipette is always adjusted for
delivery (Ex) of the selected volume.
Measuring volumes taken from balance
is not allowed. If the calculated results
are in the limits, the calibration of the
pipette is correct.
Procedure:
1. Do 10 pipettings with the minimum
volume.
2. Do 10 pipettings with the maximum
volume.
3. Calculate the accuracy (A) and
precision (cv) of both series.
4. Compare the results to the limits in the
Table 1.
If the results are in the limits of Table 1,
then the calibration of the pipette is
correct. Otherwise the pipette must be
adjusted and checked again.
Range Volume Accuracy Precision
µl µl % s.d.* µl cv%
0,5-10µl 10 ±0.10 ±1.0 0.08 0.8
1 ±0.04 ±3.5 0.03 3.0
2-20µl 20 ±0.200 ±1.0 0.080 0.4
2 ±0.060 ±3.0 0.030 1.5
5-40µl 40 ±0.240 ±0.6 0.120 0.3
5 ±0.125 ±2.5 0.100 2.0
20-200µl 200 ±1.2 ±0.6 0.6 0.3
20 ±0.6 ±3.0 0.3 1.5
100-1000µl 1000 ±5.0 ±0.5 2.0 0.2
100 ±1.5 ±1.5 0.6 0.6
1-5 ml 5000 ±25.0 ±0.5 10.0 0.2
1000 ±15.0 ±1.5 5.0 0.5
2-10 ml 10000 ±50.0 ±0.5 20.0 0.2
2000 ±20.0 ±1.0 6.0 0.3
ADJUSTMENT (Fig.10)
Adjustment is done with the service
tool.
1. Place the service tool into the
openings of the calibration nut at the top
of the handle.
2. Turn the service tool clockwise to
increase, or counterclockwise to
decrease the volume.
3. After adjustment check the calibration
according to the instructions above.

-9-
FORMULAS FOR CALCULATING
RESULTS
Conversion of mass to volume
V = (w + e) x Z
V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for mg/µl
conversion
Evaporation loss can be significant with
low volumes. To determine mass loss,
dispense water to the weighing vessel,
note the reading and start a stopwatch.
See how much the reading decreases
during 30 seconds (e.g. 6 mg = 0.2 mg/
s). Compare this to the pipetting time
from tareing to reading. Typically
pipetting time might be 10 seconds and
the mass loss is 2 mg (10s x 0.2mg/s) in
this example. If an evaporation trap or
lid on the vessel is used the correction
of evaporation is usually unnecessary.
The factor Z is for converting the
weight of the water to volume at test
temperature and pressure. A typical
value is 1.0032 µl/mg at 22°C and 95
kPa. See the conversion table on page 10.
Accuracy (systematic error)
Accuracy is the difference between
the dispensed volume and the selected
volume of a pipette.
A = V - V0A = accuracy
V = mean volume
V0= nominal volume
Accuracy can be expressed as a
relative value:
A% = 100% x A / V0
Precision (random error)
Precision refers to the repeatability of
the pipettings. It is expressed as
standard deviation (s) or coefficient of
variation (cv)
1
)( 2
-
-
=
n
VV
S
i
S
i=1
n
s = standards deviation
v = mean volume
n = number of measurements
cv is the relative value of standard
deviation.
cv = 100% x s / v

CONVERSION TABLE
Value of the conversion factor Z (µl/
mg), as a function of temperature and
pressure, for distilled water.
Temper- Air pressure
ature °C hPA (mbar)
800 853 907 960 1013 1067
15 1.0018 1.0018 1.0019 1.0019 1.0020 1.0020
15.5 1.0018 1.0018 1.0019 1.0020 1.0020 1.0021
16 1.0019 1.0020 1.0020 1.0021 1.0021 1.0022
16.5 1.0020 1.0020 1.0021 1.0022 1.0022 1.0023
17 1.0021 1.0021 1.0022 1.0022 1.0023 1.0023
17.5 1.0022 1.0022 1.0023 1.0023 1.0024 1.0024
18 1.0022 1.0023 1.0024 1.0024 1.0025 1.0025
18.5 1.0023 1.0024 1.0025 1.0025 1.0026 1.0026
19 1.0024 1.0025 1.0025 1.0026 1.0027 1.0027
19.5 1.0025 1.0026 1.0026 1.0027 1.0028 1.0028
20 1.0026 1.0027 1.0027 1.0028 1.0029 1.0029
20.5 1.0027 1.0028 1.0028 1.0029 1.0030 1.0030
21 1.0028 1.0029 1.0030 1.0030 1.0031 1.0031
21.5 1.0030 1.0030 1.0031 1.0031 1.0032 1.0032
22 1.0031 1.0031 1.0032 1.0032 1.0033 1.0033
22.5 1.0032 1.0032 1.0033 1.0033 1.0034 1.0035
23 1.0033 1.0033 1.0034 1.0035 1.0035 1.0036
23.5 1.0034 1.0035 1.0035 1.0036 1.0036 1.0037
24 1.0035 1.0036 1.0036 1.0037 1.0038 1.0038
24.5 1.0037 1.0037 1.0038 1.0038 1.0039 1.0039
25 1.0038 1.0038 1.0039 1.0039 1.0040 1.0041
25.5 1.0039 1.0040 1.0040 1.0041 1.0041 1.0042
26 1.0040 1.0041 1.0042 1.0042 1.0043 1.0043
26.5 1.0042 1.0042 1.0043 1.0043 1.0044 1.0045
27 1.0043 1.0044 1.0044 1.0045 1.0045 1.0046
27.5 1.0044 1.0045 1.0046 1.0046 1.0047 1.0047
28 1.0046 1.0046 1.0047 1.0048 1.0048 1.0049
28.5 1.0047 1.0048 1.0048 1.0049 1.0050 1.0050
29 1.0049 1.0049 1.0050 1.0050 1.0051 1.0052
29.5 1.0050 1.0051 1.0051 1.0052 1.0052 1.0053
30 1.0052 1.0052 1.0053 1.0053 1.0054 1.0055

SPARE PARTS
2201860
200-1000 µl
1130560
1130510 1131090
1053870
1053840
2202310
1131380
1053810
1053800 1053810
2x1030060
1056350
1056350
1054030
1056360
1052670
2201850
2202330
1130550
1054260
2x103017
1030020
1030230
1030160
1053860 1053860
1090610
1131710
2201520
10589070
40-200 µl
1-5 ml
0,5-10 µl
5-40 µl
1057480 for Grey
1057490 for Orange
1057500 for Yellow
1057510 for Blue
1057520 for Green
1053950
1053810
1054730
2202560 0,5-10 µl
2-20 µl
2202570 5-40 µl
20-200 µl
2202580 40-200 µl
1-5 ml
10589450

-12-
SPARE PARTS 10 ml
Labsystems
Maintenance
Pliers
2900360
Disposable Tip FT-63
Box of 40 pcs, 9402150
Bag of 100 pcs, 9402151
Tool
10589070
Tip Ejector
pusher
1053950
Cap,red
1058260
2-10 ml
Handle
2203510 Spring
1131710
Tip Ejector
Part 1
10589600
Piston
Assy
2204890
O-Ring
1033050
Cylinder
1056280
Tip Ejector
Part 2
10589620

-13-
1 5 . 0 La
1
2
1 5 0 1 0.0
Labsyste ms
3

absystems
6
1 2 3 4
A
B
C
7
absystems
4
1 2 3 4
A
B
C
5
absystems
1 2 3 4
A
B
C
absystems
A
B
C
1 2 3 4 5

Labsystems
8
9
11
Points to remove
the piston assembly
from the handle
Points to remove
the Tip ejector Part
2 from the Part 1
10

-16-
TIP ORDERING INFORMATION
Code Finntip Volume Qty
9400310 10 0,2 - 10 µl 1000/bag
9400300 10 0,2 - 10 µl 10x96/rack
9400130 200 Ext 5 - 200 µl 10x96/rack
9400260 250 Univ. 0,5 - 250 µl 10x96/rack
9401250 300 5 -300 µl 10x96/rack
9401070 1000 100 -1000 µl 200/box
9401110 1000 100-1000 µl 10x96/rack
9402070 5 ml 1-5 ml 5x54/tray
9402160 10 ml 2-10 ml 5x24/tray
1502260-03
10 ml
5 ml
1000
10
250
Universal
200 Ext
300
Product specifications are subject to change without
prior notice. Finpipette®and Finntip®are registered
trademarks of Thermo Electron Oy.
TIPS