Thermo LabSystems Multiskan RC User manual

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SERVICE MANUAL
Multiskan RC/MS/EX
Thermo Electron Corporation, Ratastie 2, P.O.Box 100, 01621 Vantaa, Finland
Telefax [phone], Tel. [phone]
280 6100
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MASTER INDEX OF
MULTISKAN RCIMSIEX
SERVICE MANUAL
BROCHURE
OPERATING INSTRUCTIONS
PCB FUNCTIONAL DESCRIPTION
BLOCK DIAGRAMS
COMPONENT LAYOUTS
SCHEMATIC DIAGRAMS
SOFTWARE
TESTING AND ADJUSTING
POWER SUPPLY
WIRING DIAGRAMS
SPARE PARTS
BULLETINS
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Multiskan RC/MS/EX Service Manual
Updates
Servicemanual version 01
-+ 02
Release 1.0 3.013.0 2.0 2.0 2.0 1.0 1.0 1.0 1.0 1.0 1.0
Chapter
1 2
3 4
5
6 7 8 9
10 II 12
Description: There has been added a hnctional descriptionof pcb MOTCU-03 (section 3),
component layouts of pcb MUSCU-2A and MUSME-01 (section
4) and a
schematic diagram ofpcb MUSCU-RC-01 (section
5).
Service manual version 02 -+ 03
Release 1.06.0/8.0/1.0 2.0 2.0 2.0 1.0 1.0 1.0 1.0 1.0 2.0
Chapter
1 2 3
4
5 6
7
8 9 10 11 12
Description: Operation manuals and Spare parts have been upgraded (section
2 and 1 I).
Multiskan EX has been added in the same manual.
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Multiskan RC/MS/EX Sewice Manual
BROCHURE
OPERATINGINSTRUCTIONS
-
Multiskan RC, Operating instructions (03.01.1997)
-
MultiskanMS, Operating instructions (14.01.1997)
-
Multiskan EX, Operating instructions (25.06.1997)
PCB FUNCTIONALDESCRIPTION
- Pcb Functional description
BLOCK DIAGRAMS
- Cover page
- Contents
- MultiskanRC, 2 pages
- Multiskan MS, 2 pages
COMPONENTLAYOUTS
- Cover page
- Contents
- SKANPRI-2
- NAPSU-2
- CHOPT-01
- MUCEN-02
- MUSME-01
- MUSCU-02A
- MOTCU-03
- MACU-2A
- MUSCU-RC-01
SCHEMATIC DIAGRAMS
- Cover page
- Contents
- SKANPIU-2
- NAPSU-2
- Filter motor assembly
- CHOPT-01
- MUCEN-02
- MUSCU - MUSME cable
- MUSME-01
- MUSCU-02A
- MOTCU-03
- Solenoid
- IR-Reader assembly
- MACU-2A
- MUSCU-RC-01
-
Serialcable Multiskan MS - MAC
-
Serial cable MultiskanMS - PC
SOFTWARE
- Cover page
- Contents
- Initialization,MultiskanRC
- Initialization,Multiskan MS
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8. TESTING AND ADJUSTING
- Cover page
- Contents
- Lamp voltage
-
Comvuter Control Commands in All Modules
-
~estkgand Adjusting for MultiskanRC
-
Testing and Adjusting for Multiskan MS
9. POWERSUPPLY
- Cover page
- Contents
-
SR 924199Parts layout, 2 pages
- SR 924100 Schematicdiagram
10. WIRINGDIAGRAMS
Cover page
Contents
Track cover grounding wire
Power cable
Filter motor cable
Fan assembly
LCD display
Lamp cable
CHOPT
- Power
CHOPT
- MIJSCU
CHOPT-motor
MUSCU
- MUSME
Track motor grounding wire
Solenoid
IR-Reader assembly
Hall sensor assembly
Groundingwire
Serial cableMultiskan MS
- MAC
Serial cableMultiskan MS
- PC
Groundingwire
Groundingwire
RS-232C
Serialcable PC
Serial cableMAC
11. SPARE PARTS
-
Multiskan RCIMSIEX Sparepart list
12. BULLETINS
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Proven microplate reader with exceptional flexibiliry
[bB
Provenoptic:il system guarantees accurate and rcliable results
Exception;il flcxihilily thn)iigh choice ofoptiotis
Selection of up to
8 different lilters
Optics[or visible
or I1V visihlc arca
a
Agglutin:ttion nieasurenient system 2s option
Two hasic models: PC contsolled or with built-insoftware
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The NewMultiskanFamily - the provcn Technical spccifiarlions
~ ~
industrystandard for microplate readers
now oifers supcrior flexibility Optical specifimtions
Wirhont wmpromising
on any of
*p"""l r"nge
ilcpends on the optcrlsyhtcin dccrcd 100-75C nrn or )iIlb7Vi iim
Krldk,", ranxc: 114 5 A
the qwlitietlxrt have helped Multiskan
~~~~~,~~~~~~,t nnli.c: (1-2 A rr coniinimus mode. 0-2.5 A st sen nrixic
set the indusrrv stmdarcl, 1;absvstems
Accoiac~:
~2% or t) 007 A, cypicat V;~IC~~. il'm
has nowdevclopeci a Ncw Multiskan
'"""'"":
CVcO5"h ooi UlW7 A, ryps:d v.$lue.CVa1.2%
l.inc?rity:
ti% oillo07 A, lypicrl v;iluc +ISM
Family to oftkr the customer the great-
cst
flexibility :rv:iillhle.
The New Multiskan Fanlily consists
dtwr, l,asic
rader models: Muliisknrr
IK mil Multiskan MS. Multis1t:ln RC is
fully
comp~itel--rontr<)llerl. Multiskan MS
rrmcs with 1,uilt-in software.
t3y selecting the dcsircd Ik~sicredder
rnodcl :ind the crmbinatkm oioptions,
the
user c:m <,ptimize the unit's
perfrx~naticeaccording to his or her
work.
Options for rlie
Ncw Multisknn
I::imily include
:I cl~oice oC up to K dif-
ferent filters. oplics
fix visil~leor in/
area and ;in :tgglutin'ation measurcmcnt
system
For Multisk:m MS ;I specially drsign-
ed primer :~nd:iddition;~lct~toff :ind/or
wl,ic spline progr:ims ;IK :iv;iil;ll,lc.
All Multiskm t~loilels;ire light-
weight, ctm,p:icr instl-~imcnlsilcsigncd
to suit every i:ilwr:itory
I.;hsyten~b
oilers powerful cxtcrnal
soI'cw:irc for M:~cintoslr.
1)OS end
Ordering information
-1 1,e..c,iji,""'
b1~cI~i~h:mIll:
51 1 lHliO7
Muhk.~I!<:, IOUI~OV
51 1 IHli17
MU/<I&.III IlC. 2lIlI-L4llV
&W --- lIe,c,#~
hliillirk;tn MS ojilimr
il I lHiM0 Ag#l~$~irutkmoptics
51 I IHOiO IIV <>pic>
51 I IHO(*i I'linirr
ill
iXO70 C,,li,l'i linlgrnie .arlolc
51 1 IHOHO Cc#l,ics~,iiilc~pnqv:un#~IMUIC
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Information in this manual is subject to change without prior notice.
Cat.
no 1506250
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LABSYSTEMS WTI
CONTENTS
SAFETY SYMBOLS GS
I. LPISTALLATION
1.1 Unpacking
1.2 EnvironmenW RequiremenLs
1.3 Installation
1.4 Transporting
for Service
2. FUNCTIONAL DESCR
2.1 Inuoduction
2.2 MeasurementRinciple
2.2.1 Photometric Measwemen@
2.2.2 Agglurination Measuremenu
2.3 InsnunlentLayout
3. OPERATION
3.1 Now
to Gets
1.2 Computer Control Commands
3.3 Achowledgemenc Messages
3.4 &or Messages
4. SERVTCLNC
4.1 Maintenance
4.2 Routine Cleaning
of the Insbrument
4.3 Cleaning
of the Optical Syswm
4.4 Disinfection
4.7 Changing &e
APPEND
Ap 1.
Appendix 2.
Appendix 3.
Ordering Infomarion and Addresses
Page
2
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0
Power OFF
Caution, risk of personal injmy to Lhe operator o~. a safety hazard for the smoundmg
Caution,
risk
ge to the inshumene, othgr eg~bipnrent m loss of
function in a application.
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@ RC carefullyandcheckPor any
agethat may havebcencauM during rrans
part is damaged or missing. contact the local Labsystemsrepresenlative.
Keep the original package for future mspamrion.
XC to avoid exposure to excess dust, vibrations. strongmagnetic fields, disecr sunlight, draft,
excessivemoisture OK large ternpr;tlwe Ructuations.
Leave sufficientclearance
(10 cm) at both sidesof the unit for adquace air circularion.
Remove the two cover relairing screws(Fig.12)
and ljft up the cover.
1. lnsburnentcover
2. Retainingscrewsof the insmment cover
3. Measuring assembly cover
4. Reraining screwsof the measwing assemblycover
5. Plale carrier
6. Transfa mils
9. Track
Figure
1. Multiskan RC
Unpack the filter wheel. Chwk thar all filmsare clan and undamaged. Plx 1 inlathe filer slo( (Fig. 25)
so that the toothededge faces the rear of heinsvurnent. A magnetic locking mwill automarically lack the
wheel in thccomeccposition and heoptical filter position sensor will rn
measuaemenl
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1. Lamp
2. Chopper
3. Filter
4. Fan
5. Filmwhecl slot
6. ssor ckuit
7. Serial RS-232C DL switches
Figure
2.
Multiskan RC internal view
NOTE:
Do not toush the reflacrive surface oh the Lamp or the bulb iklf,
Unpack the lamp. Fit the lamp contacts to the terminal socket inside the insmment (Fiy.3).
Place the lamp in its place (Fig. 21).
Figure 3. Inscallation of lamp
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Check visually has there are no loose inside the insmment.
Close rhe insmmentcover
md replacethe reraining screws.
Multiskan
RCdoes not preduceoperatkg noiseaialevel whichwuld cause8hazard. Nosound level measlaemenrs
m needed afterinsrallation.
Connect the mains supply cord
tv the mains power socket Fig. 42). The power supply has automatic 1WI20 /
200-240 VAC range selection. If a suitablernains supply cord is not provided, use only cords certified by rhe id
authorities.
Connectthe insmmentto acomecdyinstaltcdIinepowerouUeiwhich
hasaprowtiveconductoralsocdled as earth
or ground.
CAUTION:
Interruptionof theprotec6ve eon uclor insideoroueidethe i
enlordisconnection of
the protective conductor terminal may make the instru
1. Power switch
2. Power input socket
3. Fuses
nnectoc for a computer
Comect a cornpureraccordin uiamenu. For instructions about the inkdacesysern.
Repack
the instrument according la insuuctionsenclod in Lhc package. Use the originalpac ge. India&the fault.
Inform about the use of
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Mulliskan RC is m eiat-channelverticallightpath filarphotom%terdesigned to perform bath stm
and agglutinationmeasuremenu under the conmlof a computer.
Thewavelength
(400 - 750 nm oroptional 340 - 450 nm) is seler. using maximum eight high qualiky intedercnce
filters
held in a filter wheel.
The readings
can be processed via a serial RS-242C interface.
2.2.1 Photometric
MultimRCutiliza
theoriginallabsystemskonceptofverlic2il phommew inwhich thehghtbeam passes through
he wholesample.
In verticalphotomeQ,theabsorption of light
is pmporaional to theamountof lightabsorbing substance in the well
Absorption
is expressed by \he following equation:
whem
A = absorbance, a = molar absorptivity of the substance,m = mass of absorbing substance and S = cross-
sectional
aaea pergendicular to the light pa&.
The advanages of (he venialtight path measurcmms. are:
(I)
inaccurate pigetling of nonabsorbing liquids does not alfec.6 Lhe measu absorbancevalues
(2) evapmtion of nonabsorbing tiqlaids duping the cion dmnot dfar ahsohankvalues and
(4) acemhdegreeofinhomage ityin ~solution.forexampleasarcsultoflayering in turbiditynwaurernenrs.
does not
affect (he results.
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The optical system comprises hefollowingcomponenrs(Fig. 5a):
1 quartz-halogen lamp
2 chopper wheel
3 apeme and condenserlens
4 semi-mnsparenr mirror
5 apeme
6 inteplerenffifilter (filler wheel)
7 optical fibrebundle
8 focusing lenses
9 upper lensesand detectors
Figure
5a. Optical system
Light source:
Toprolong the service life of the lamp, &e hsuumenl should
of
1 minute has IO be allowed before measuring.
Chopper wheel:
Thc chopper wheelchops the light
lo minimize elecmnic noise.
Afterpassing through hecondenwr lensthe light aenr
mirror. Bartofthe visible
lightand aU longer wavelengthspass lhrough the visibletight kreflec
arrangementeliminates
hearing of the interferen tensity Bisuibucion.
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The wavelength is selec
born (he maximrun of eight filtersheld in the filter wheel.
Fibre bundte:
ter, thelight thecommon endof thefibrebundle,whichdivides
s and defles upwapds.
Focusing lenses:
After being divided the light passes through a focusingsystem of eight lenses.
Detectors:
Thelight
kams pass through the bouomsof thewells though the samples via up
r lenses to the Bemtom, wh~h
measurethe intensity of lighr,
2.2.2 Agglutination
Agglulinationmeasmemenu
are
rmedby using special optics(pinholeoptics) (Fig. Sb). Tke insmunent scms
overeach well measuring a 29 pinuwell pmfile taking 20 readings. Readings are then down-loaded to a PC. for
fdie~ interpre&tion.
I. Focusing lenses
2. Upper lenses and dececmrs
3. Pinhole plate
Fig. Sb. Agglutinationoptics
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Instrumen6cover
Reraining screws of he insmmenr cover
Measuring assemblycover
Retaining screwsoi'thc
measuring assemblycover
Place carrier
Transfer rails
Track
Status LED, thm colola
Figure 6. Multiskan RC
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1. Lamp
2. Chopper wheel
3. Fitter
4. Fan
5. Filter wheel slot
6.
MUSCU - RC processor bard
7. Scpial RS-232C DL switches
Figure
7.
Multiskan RC internal view
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1. Power switch
2. Power input
3. Fuses
4.
Volfage selector switch (if exist)
5.
RS-232C inleefax cannecror for a computer
Figure 8. Multi
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