PerkinElmer LAMBDA 265 User manual

LAMBDA 265, 365 AND 465
ASX-260/280 AND ASX-
520/560 AUTOSAMPLER
Users Guide
UV SPECTROMETERS

2 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
Release History
Part Number Release Publication Date
09931308 C September 2016
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer
710 Bridgeport Avenue
Shelton, CT 06484-4794
U.S.A.
Or emailed to: [email protected]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no
warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in
connection with furnishing, performance or use of this material.
Copyright Information
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translated into any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2016 PerkinElmer, Inc.
Trademarks
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are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.

Contents . 3
Table of Contents

Introduction......................................................................................5
Features................................................................................................6
Autosamplers and Peristalic Pump............................................................7
Dimensions and Specifications.................................................................8
Dimensions of AutoSampler ..............................................................8
Specifications of Peristaltic Pump.......................................................8
Safety Summary.....................................................................................9
Conventions Used in this Manual............................................................12
Notes, cautions and warnings..........................................................12
Note, attention et avertissement .....................................................12
Contact Us...........................................................................................15
Description......................................................................................17
Description of the Autosampler..............................................................18
Front View of Autosampler..............................................................18
Back View of Autosampler...............................................................19
Description of the Peristaltic Pump.........................................................20
Installation......................................................................................23
Installation...........................................................................................24
Interface Setup...............................................................................33
Installing the Driver of Lambda 465 .......................................................34
Setting USB Serial Ports ........................................................................36
UV Lab Software .............................................................................41
Wavelength Monitoring Measurement Procedure.....................................42
Quantification Measurement Procedure...................................................47
UV Express Software.......................................................................53
Scan and Wavelength Program Mode Measurement Procedure.................54
Quantification and Scanning Quantification Mode Measurement Procedure59
Maintenance....................................................................................65
Maintenance ........................................................................................66

4 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide

Introduction

6 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
Features
The ASX 260/280 and ASX-520/560 autosamplers are designed to be study, reliable and easy
to use. They provide automated sample introduction that enables you to perform other tasks
while the Auto Sampler runs.
The ASX 260/280 and ASX-520/560 automatically introduces up to 180 and 360 samples
each when fully loaded. It contains a microprocessor that allows sequential or random
sampling, providing flexibility.
The components of autosampler are made of corrosion-resistant stainless steel alloys or
anodized aluminum. The enclosure and base are made from a high-strength aluminum alloy
that is chromated and finished with an epoxy powder coating.
The autosampler operates reliably under a wide variety of conditions. Components in the
sample flow path are made of polyetherimide (PEI) and polytetrafluoroethylene (PTFE).
When these inert, non-metallic materials are used at temperatures less than 135 °C, they
can withstand repeated exposure to the following substances.

Autosamplers and Peristalic Pump
Autosampler (ASX-260) Autosampler (ASX-520)
Autosampler (ASX-280) Autosampler (ASX-560)
Peristaltic Pump

8 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
Dimensions and Specifications
Dimensions of AutoSampler
Physical
Characteristic Specification
ASX-260 ASX-520
Power 100-240 VAC, 47/63 Hz
Dimensions (mm) 330 (W) x 508 (D) x 610 (H) 520 (W) x 482 (D) x 610 (H)
Weight (kg) 8.4 10.5
Tray 2 racks capacity 4 racks capacity
Physical
Characteristic Specification
ASX-280 ASX-560
Power 100-240 VAC, 47/63 Hz
Dimensions (mm) 355 (W) x 550 (D) x 620 (H) 580 (W) x 550 (D) x 620 (H)
Weight (kg) 8.1 11.7
Tray 2 racks capacity 4 racks capacity
Specifications of Peristaltic Pump
Specifications
Speed (rpm) 0.1 to 100
Flow rate* 0.0002 - 35 ml/min/channel (tubing dependent)
Motor type Stepper motors
Channels 2
Tubing i.d.(mm) 0.13 to 3.17
Temperature, operating (°C) -5 to 40
Humidity Up to 80%
Dimensions (mm) 170 (H) x 125 (W) x 193 (D)
Power 30 W, 100 – 240 VAC, 50/60 Hz
Weight (kg) 2.7

Safety Summary
The following safety symbols are used on this product.
Warning Description
Always refer to the system manual when working near locations at
which the alert mark shown on the left is attached. If the operation,
etc., is performed without heeding the advice in the System manual,
there is a risk of personal injury. In addition, the equipment
performance may be reduced. Moreover, this alert mark is sometimes
used with other marks and descriptions indicating other dangers.
Il faut toujours voir le manuel du système lors de l'exécution de travaux
près des endroits où le panneau d’alerte montré dans la colonne à
gauche est placé. Il y a un risque de blessures, si le travail est effectué
sans respecter les conseils dans le manuel du système. En outre, le
rendement de l'équipement pourrait être réduit. Par ailleurs, ce
panneau d’alerte est parfois utilisé avec d'autres panneaux comportant
les indications de danger.
Warning
When supplying power to this equipment, connect the accessory 3-pin
power cord to a 3-pin grounded power outlet. If a grounded 3-pin
outlet is not available, use a conversion adapter and ground the green
wire. If power is supplied without grounding the equipment, there is a
risk of receiving a fatal electric shock and equipment damage.
Lors de l'alimentation de l’équipement, branchez le cordon
d'alimentation accessoire à 3 broches dans une prise d’alimentation
mise à la terre à 3 broches. Si une prise mise à la terre à 3 broches
n’est pas disponible, utilisez un adaptateur de conversion en raccordant
le fil vert pour réaliser la mise à la terre. Si l'alimentation est fournie
sans mettre l'équipement à la terre, il y a un risque d’électrocution et
de dommages à l’équipement.
“On” (power)
To indicate connection to the mains, at least for mains switches or their
positions, and all those cases where safety is involves.
"On" (Sous tension)
Pour indiquer le branchement à l’alimentation du secteur, au moins
pour les interrupteurs du secteur ou leur position, et tous les autres cas
qui concernent la sécurité.

10 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
“OFF” (power)
To indicate disconnection from the mains, at least for mains switches or
their positions, and all those case where safety is involves.
"OFF" (Hors tension)
Pour indiquer la coupure du secteur, au moins pour les interrupteurs du
secteur ou leur position, et tous les autres cas qui concernent la
sécurité.
Repair
Warning
The user cannot repair this equipment.
DO NOT attempt to open the case or to disassemble internal parts.
Only your PerkinElmer service representative with knowledge of
electrical fire and shock hazard should service this equipment.
There are high-voltage parts in this equipment presenting a risk of
severe injury or fatal electric shock to untrained personnel.
In addition, there is a risk of damage to precision parts.
L'utilisateur ne doit pas essayer de réparer cet équipement.
NE PAS tenter d'ouvrir le boîtier ou de démonter ses pièces internes.
Seul un représentant du service PerkinElmer qui est conscient des
risques d'incendie et d’électrocution devrait réparer cet équipement.
Cet équipement contient des parties à haute tension présentant des
risques de blessure grave ou d’électrocution au personnel non qualifié.
En outre, il existe un risque d'endommagement des pièces de précision.
Falling Over
Caution
This equipment should be used in the correct position. If the equipment
is turned on its side, etc., it will be unstable and may be damaged if it
falls over as a result of receiving a slight mechanical shock.
Cet équipement doit être utilisé dans l’orientation correcte. Si, par
exemple, l'équipement est couché sur un de ses côtés, il deviendra
instable. S’il tombe à la suite d'un léger choc mécanique, il pourra être
endommagé.