PerkinElmer LAMBDA 265 User manual

LAMBDA 265, 365 AND 465
ASX-260/280 AND ASX-
520/560 AUTOSAMPLER
Users Guide
UV SPECTROMETERS

2 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
Release History
Part Number Release Publication Date
09931308 C September 2016
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer
710 Bridgeport Avenue
Shelton, CT 06484-4794
U.S.A.
Or emailed to: [email protected]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no
warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in
connection with furnishing, performance or use of this material.
Copyright Information
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translated into any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2016 PerkinElmer, Inc.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically marked as such,
are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.

Contents . 3
Table of Contents

Introduction......................................................................................5
Features................................................................................................6
Autosamplers and Peristalic Pump............................................................7
Dimensions and Specifications.................................................................8
Dimensions of AutoSampler ..............................................................8
Specifications of Peristaltic Pump.......................................................8
Safety Summary.....................................................................................9
Conventions Used in this Manual............................................................12
Notes, cautions and warnings..........................................................12
Note, attention et avertissement .....................................................12
Contact Us...........................................................................................15
Description......................................................................................17
Description of the Autosampler..............................................................18
Front View of Autosampler..............................................................18
Back View of Autosampler...............................................................19
Description of the Peristaltic Pump.........................................................20
Installation......................................................................................23
Installation...........................................................................................24
Interface Setup...............................................................................33
Installing the Driver of Lambda 465 .......................................................34
Setting USB Serial Ports ........................................................................36
UV Lab Software .............................................................................41
Wavelength Monitoring Measurement Procedure.....................................42
Quantification Measurement Procedure...................................................47
UV Express Software.......................................................................53
Scan and Wavelength Program Mode Measurement Procedure.................54
Quantification and Scanning Quantification Mode Measurement Procedure59
Maintenance....................................................................................65
Maintenance ........................................................................................66

4 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide

Introduction

6 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
Features
The ASX 260/280 and ASX-520/560 autosamplers are designed to be study, reliable and easy
to use. They provide automated sample introduction that enables you to perform other tasks
while the Auto Sampler runs.
The ASX 260/280 and ASX-520/560 automatically introduces up to 180 and 360 samples
each when fully loaded. It contains a microprocessor that allows sequential or random
sampling, providing flexibility.
The components of autosampler are made of corrosion-resistant stainless steel alloys or
anodized aluminum. The enclosure and base are made from a high-strength aluminum alloy
that is chromated and finished with an epoxy powder coating.
The autosampler operates reliably under a wide variety of conditions. Components in the
sample flow path are made of polyetherimide (PEI) and polytetrafluoroethylene (PTFE).
When these inert, non-metallic materials are used at temperatures less than 135 °C, they
can withstand repeated exposure to the following substances.

Autosamplers and Peristalic Pump
Autosampler (ASX-260) Autosampler (ASX-520)
Autosampler (ASX-280) Autosampler (ASX-560)
Peristaltic Pump

8 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
Dimensions and Specifications
Dimensions of AutoSampler
Physical
Characteristic Specification
ASX-260 ASX-520
Power 100-240 VAC, 47/63 Hz
Dimensions (mm) 330 (W) x 508 (D) x 610 (H) 520 (W) x 482 (D) x 610 (H)
Weight (kg) 8.4 10.5
Tray 2 racks capacity 4 racks capacity
Physical
Characteristic Specification
ASX-280 ASX-560
Power 100-240 VAC, 47/63 Hz
Dimensions (mm) 355 (W) x 550 (D) x 620 (H) 580 (W) x 550 (D) x 620 (H)
Weight (kg) 8.1 11.7
Tray 2 racks capacity 4 racks capacity
Specifications of Peristaltic Pump
Specifications
Speed (rpm) 0.1 to 100
Flow rate* 0.0002 - 35 ml/min/channel (tubing dependent)
Motor type Stepper motors
Channels 2
Tubing i.d.(mm) 0.13 to 3.17
Temperature, operating (°C) -5 to 40
Humidity Up to 80%
Dimensions (mm) 170 (H) x 125 (W) x 193 (D)
Power 30 W, 100 – 240 VAC, 50/60 Hz
Weight (kg) 2.7

Safety Summary
The following safety symbols are used on this product.
Warning Description
Always refer to the system manual when working near locations at
which the alert mark shown on the left is attached. If the operation,
etc., is performed without heeding the advice in the System manual,
there is a risk of personal injury. In addition, the equipment
performance may be reduced. Moreover, this alert mark is sometimes
used with other marks and descriptions indicating other dangers.
Il faut toujours voir le manuel du système lors de l'exécution de travaux
près des endroits où le panneau d’alerte montré dans la colonne à
gauche est placé. Il y a un risque de blessures, si le travail est effectué
sans respecter les conseils dans le manuel du système. En outre, le
rendement de l'équipement pourrait être réduit. Par ailleurs, ce
panneau d’alerte est parfois utilisé avec d'autres panneaux comportant
les indications de danger.
Warning
When supplying power to this equipment, connect the accessory 3-pin
power cord to a 3-pin grounded power outlet. If a grounded 3-pin
outlet is not available, use a conversion adapter and ground the green
wire. If power is supplied without grounding the equipment, there is a
risk of receiving a fatal electric shock and equipment damage.
Lors de l'alimentation de l’équipement, branchez le cordon
d'alimentation accessoire à 3 broches dans une prise d’alimentation
mise à la terre à 3 broches. Si une prise mise à la terre à 3 broches
n’est pas disponible, utilisez un adaptateur de conversion en raccordant
le fil vert pour réaliser la mise à la terre. Si l'alimentation est fournie
sans mettre l'équipement à la terre, il y a un risque d’électrocution et
de dommages à l’équipement.
“On” (power)
To indicate connection to the mains, at least for mains switches or their
positions, and all those cases where safety is involves.
"On" (Sous tension)
Pour indiquer le branchement à l’alimentation du secteur, au moins
pour les interrupteurs du secteur ou leur position, et tous les autres cas
qui concernent la sécurité.

10 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
“OFF” (power)
To indicate disconnection from the mains, at least for mains switches or
their positions, and all those case where safety is involves.
"OFF" (Hors tension)
Pour indiquer la coupure du secteur, au moins pour les interrupteurs du
secteur ou leur position, et tous les autres cas qui concernent la
sécurité.
Repair
Warning
The user cannot repair this equipment.
DO NOT attempt to open the case or to disassemble internal parts.
Only your PerkinElmer service representative with knowledge of
electrical fire and shock hazard should service this equipment.
There are high-voltage parts in this equipment presenting a risk of
severe injury or fatal electric shock to untrained personnel.
In addition, there is a risk of damage to precision parts.
L'utilisateur ne doit pas essayer de réparer cet équipement.
NE PAS tenter d'ouvrir le boîtier ou de démonter ses pièces internes.
Seul un représentant du service PerkinElmer qui est conscient des
risques d'incendie et d’électrocution devrait réparer cet équipement.
Cet équipement contient des parties à haute tension présentant des
risques de blessure grave ou d’électrocution au personnel non qualifié.
En outre, il existe un risque d'endommagement des pièces de précision.
Falling Over
Caution
This equipment should be used in the correct position. If the equipment
is turned on its side, etc., it will be unstable and may be damaged if it
falls over as a result of receiving a slight mechanical shock.
Cet équipement doit être utilisé dans l’orientation correcte. Si, par
exemple, l'équipement est couché sur un de ses côtés, il deviendra
instable. S’il tombe à la suite d'un léger choc mécanique, il pourra être
endommagé.

Warning Description
Cleaning
Caution
Keep the equipment free of dust.
Clean the power line regularly; if dust accumulates around the power
pins, there is a risk of fire.
Keep the equipment clean so that the ventilation holds are not
obstructed. If the ventilation is obstructed, the system may overheat
and catch fire.
Periodically clean your equipment's case using a damp cloth.
Do not use abrasives, cleaning solvents or strong detergents, as they
may damage the finish or affect the reliability of the structural
components.
Gardez l'équipement exempt de poussières.
Nettoyez le cordon d'alimentation régulièrement. Les poussières qui
s’accumulent autour des broches du cordon d'alimentation pourraient
entraîner un risque d'incendie.
Gardez l'équipement propre de sorte que les orifices de ventilation ne
soient pas bouchés. Si les grilles de ventilation sont obstruées, le
système pourrait surchauffer et s'enflammer.
Nettoyez périodiquement le boîtier de l'équipement à l'aide d'un chiffon
humide.
If this equipment is used in a manner not specified by this manual, the
protection provided by the equipment may be impaired.
Si l’équipement est utilisé d'une manière non spécifiée dans ce manuel,
la protection fournie par l'équipement risque d’être altérée

12 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
Conventions Used in this Manual
Normal text is used to provide information and instructions.
Bold text refers to text that is displayed on the screen.
UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. '+' is used
to show that you have to press two keys at the same time, for example, ALT+F.
All eight digit numbers are PerkinElmer part numbers unless stated otherwise.
Notes, cautions and warnings
Three terms, in the following standard formats, are also used to highlight special
circumstances and warnings.
NOTE:
A note indicates additional, significant information that is provided with some procedures.
Note, attention et avertissement
Trois termes, dans les formats standard suivants, sont également utilisés pour mettre des
circonstances et avertissements spéciaux en évidence.
NOTE:
Une note indique des renseignements supplémentaires et significatifs qui sont fournis avec
certaines procédures.

CAUTION
We use the term CAUTION to inform you about situations that
could result in serious damage to the instrument or other
equipment. Details about these circumstances are in a box like
this one.
D Caution (Achtung)
Bedeutet, daß die genannte Anleitung genau befolgt werden muß,
um einen Geräteschaden zu vermeiden.
DK Caution (Bemærk)
Dette betyder, at den nævnte vejledning skal overholdes nøje for
at undgå en beskadigelse af apparatet.
E Caution (Advertencia)
Utilizamos el término CAUTION (ADVERTENCIA) para advertir
sobre situaciones que pueden provocar averías graves en este
equipo o en otros. En recuadros éste se proporciona información
sobre este tipo de circunstancias.
F Caution (Attention)
Nous utilisons le terme CAUTION (ATTENTION) pour signaler les
situations susceptibles de provoquer de graves détériorations
de l'instrument ou d'autre matériel. Les détails sur ces
circonstances figurent dans un encadré semblable à celui-ci.
I Caution (Attenzione)
Con il termine CAUTION (ATTENZIONE) vengono segnalate
situazioni che potrebbero arrecare gravi danni allo strumento
o ad altra apparecchiatura. Troverete informazioni su tali
circostanze in un riquadro come questo.
NL Caution (Opgelet)
Betekent dat de genoemde handleiding nauwkeurig moet worden
opgevolgd, om beschadiging van het instrument te
voorkomen.
P Caution (Atenção)
Significa que a instrução referida tem de ser respeitada para
evitar a danificação do aparelho.

14 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
WARNING
We use the term WARNING to inform you about situations that
could result in personal injury to yourself or other persons.
Details about these circumstances are in a box like this one.
D Warning (Warnung)
Bedeutet, daß es bei Nichtbeachten der genannten Anweisung
zu einer Verletzung des Benutzers kommen kann.
DK Warning (Advarsel)
Betyder, at brugeren kan blive kvæstet, hvis anvisningen ikke
overholdes.
E Warning (Peligro)
Utilizamos el término WARNING (PELIGRO) para informarle
sobre situaciones que pueden provocar daños personales a
usted o a otras personas. En los recuadros como éste se
proporciona información sobre este tipo de circunstancias.
F Warning (Danger)
Nous utilisons la formule WARNING (DANGER) pour avertir
des situations pouvant occasionner des dommages corporels
à l'utilisateur ou à d'autres personnes. Les détails sur ces
circonstances sont données dans un encadré semblable à
celui-ci.
I Warning (Pericolo)
Con il termine WARNING (PERICOLO) vengono segnalate
situazioni che potrebbero provocare incidenti alle persone.
Troverete informazioni su tali circostanze in un riquadro come
questo.
NL Warning (Waarschuwing)
Betekent dat, wanneer de genoemde aanwijzing niet in acht
wordt genomen, dit kan leiden tot verwondingen van de
gebruiker.
P Warning (Aviso)
Significa que a não observância da instrução referida poderá
causar um ferimento
ao usuário.

Contact Us
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using
the part numbers quoted in the guides provided with the instrument.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for
information:
If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your
order will be shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.

16 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide

Description

18 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
Description of the Autosampler
Front View of Autosampler
A) Sample tray The sample tray holds the rinse station, sample racks, and standards
vials in place. The tray also contains small spills.
B) Sample vial racks The ASX-520/560 includes four sample vial racks; the ASX-
260/280 includes two racks. You can choose from different rack sizes (common sizes fit
21, 24, 40, 60 or 90 vials per rack). You can combine racks of different sizes as long as
the host computer’s software accepts the combination.
C) Standards vials Ten standards vials are included with the autosampler. The
standards vials, which fit into the standards positions at the back of the sample tray, are
50-milliliter conical centrifuge vials with caps.
D) Rinse station
The flowing rinse station is located at the left end of the standards positions at the back
of the sample base. It comes with tubing used to connect the rinse station to the rinse
source and the waste container.
E) Arm The arm moves the sample probe horizontally
F) Z-Drive assembly The z-drive assembly includes a y-axis slider block and guide
plate as well as the sample probe. The z-drive assembly fits onto the autosampler arm.
G) Power Indicator Lamp The LED indicates that the autosampler is connected to a
power source and turned on.

Description. 19
Back View of Autosampler
A) Peristaltic pump
A two-channel peristaltic pump moves the rinse solution from the rinse source through
the flowing rinse station.
B) Serial Ports
The COM1 port connects the autosampler with the analytical instrument’s host
computer. The COM2 port connects the autosampler to other external devices, such as
an autodilutor.
C) USB Port The USB port can be used to interface the autosampler with the host
computer.

20 . Lambda 265, 365 and 465 ASX-260/280 AND ASX-520/560 Autosampler Users Guide
Description of the Auto sipper
Peristaltic Pump for Lambda 265/365/465
Lambda 265/465
Tube connection components for Lambda 265/465
Conical
Adapter
(2ea)
Outlet tube
(1ea)
Peristaltic
pump tubing
(1ea)
Inlet tube
(1ea)
Tube 1.5 M
(1ea)
Flow cell (1ea)
Beam height:
L265(8.5 mm)
L465(15 mm)
- Flow cell for L265: P/N N4101051
- Flow cell for L465: P/N N4101052