ThermoFisher Scientific thermoscientific Cryofuge 5500i User manual

Thermo Scientific
Cryofuge 5500i
Instruction Manual
20057781-f • 07 / 2020
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WEEE Conformity
This product is subject to the regulations of the EU Waste Electrical & Electronic
Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:

Thermo Scientific Cryofuge 5500i i
T
Preface ....................................................................................................................................... iii
Scope of Supply ....................................................................................................................... iii
Intended Use ..............................................................................................................................iv
Accident Prevention .................................................................................................................iv
Precautions ................................................................................................................................iv
Chapter 1 Introduction and Description ............................................................................................... 1-1
Characteristics of the Cryofuge 5500i ...................................................................... 1-2
Technical Data ........................................................................................................ 1-3
Directives, Standards and Guidelines ...................................................................................... 1-4
Functions and Features ............................................................................................................ 1-4
Mains Supply ........................................................................................................... 1-5
Refrigerants .............................................................................................................. 1-6
Chapter 2 Before Use ............................................................................................................................... 2-1
Before Setting up ..................................................................................................... 2-2
Setting up ................................................................................................................ 2-2
Aligning the Centrifuge ........................................................................................... 2-4
Transporting the Centrifuge .................................................................................... 2-4
Location .................................................................................................................. 2-4
Mains Connection ................................................................................................... 2-5
Adjust Voltage and Frequency ................................................................................. 2-6
Storage ..................................................................................................................... 2-6
Chapter 3 Control Panel .......................................................................................................................... 3-1
Control Panel .......................................................................................................... 3-2
Keys ......................................................................................................................... 3-2
Chapter 4 Operation ................................................................................................................................. 4-1
Switch on Centrifuge ............................................................................................... 4-2
Centrifuge Door ..................................................................................................... 4-2
Rotor Installation ..................................................................................................... 4-3
Entering Parameters ................................................................................................. 4-4
Centrifugation ......................................................................................................... 4-8
Temperature Adaptation during Standstill ............................................................... 4-9
Short-term Centrifugation ....................................................................................... 4-9
Removing the Rotor ................................................................................................ 4-9
Aligning the Centrifuge ......................................................................................... 4-10
Audible Alarm ....................................................................................................... 4-10
Key Switch ............................................................................................................. 4-11
Table of Contents
Rotor Selection ........................................................................................................ 1-6

Contents
ii Cryofuge 5500i Thermo Scientific
Chapter 5 Maintenance and Care ......................................................................................................... 5-1
Cleaning Intervals .................................................................................................... 5-2
Cleaning .................................................................................................................. 5-2
Cleaning the Venting Slots ...................................................................................... 5-3
Disinfection ............................................................................................................. 5-3
Decontamination ..................................................................................................... 5-4
Service of Thermo Fisher Scientific .......................................................................... 5-5
Chapter 6 Troubleshooting ..................................................................................................................... 6-1
Mechanical Emergency Door Release ...................................................................... 6-2
Troubleshooting by User ......................................................................................... 6-3
When to contact a Service Technician ..................................................................... 6-5

Thermo Scientific Cryofuge 5500i iii
P
Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the
instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the seller's warranty.
Scope of Supply
If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.
Article Number Quantity Check
Centrifuge Cryofuge 5500i 1
Power supply cable 1
50127698 CD with manual 1
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

PIntended Use
iv Cryofuge 5500i Thermo Scientific
Intended Use
•This centrifuge is a laboratory product designed to separate components by generation of a
relative centrifugal force. It separates human samples (e.g. blood, urine and other body fluids)
collected in appropriate containers, either alone or after addition of reagents or other additives.
•Laboratory centrifuge for the application in large laboratories, blood banks, and other the sector
of the pharmaceutical and bio-technological industry.
•The centrifuge is to be used for separating materials of different density or particle size
suspended in a liquid.
Maximum sample density at maximum speed: 1.2
Accident Prevention
Precautions
g
cm3
-----------
Prerequisite for the safe operation of the Cryofuge 5500i is a work environment in
compliance with standards, directives and trade association safety regulations and proper
instruction of the user.
The safety regulations contain the following basic recommendations:
•Maintain a radius of at least 30 cm around the centrifuge.
•Implementation of special measures which ensure that no one can approach the
centrifuge for longer than absolutely necessary while it is running.
•The mains plug must be freely accessible at all times. Pull out the power supply plug or
disconnect the power supply in an emergency.
In order to ensure safe operation of the Cryofuge 5500i, the following general safety
regulations must be followed:
•The centrifuge should be operated by trained specialists only.
•The centrifuge is to be used for its intended use only.

P Precautions
Thermo Scientific Cryofuge 5500i v
•Plug the centrifuge only into sockets which have been properly grounded.
•Do not move the centrifuge while it is running.
•Do not lean on the centrifuge.
•Use only rotors and accessories for this centrifuge which have been approved by Thermo
Fisher Scientific. Exceptions to this rule are commercially available glass or plastic
centrifuge tubes, provided they have been approved for the speed or the RCF value of
the rotor.
•Do not use rotors which showany signs of corrosion and / or cracks.
•Do not touch the mechanical components of the rotor and do not make any changes to
the mechanical components.
•Use only with rotors which have been properly installed. Follow the instructions in
section “Rotor Installation” on page 4-3.
•Use only withrotors which have been loaded properly. Follow the instructions given in
the rotor manual.
•Never overload the rotor. Follow the instructions given in the rotor manual.
•Never open the lid until the rotor has come to a complete stop and this has been
confirmed in the display.
•The lid emergency release may be used in emergencies only to recover the samples from
the centrifuge, e.g. during a power failure
(see section “Mechanical Emergency Door Release” on page 6-2).
•The performance of the gas springs might diminish after some time. Check the gas
springs as described in “Centrifuge Door” on page 4-2.
•Never use the centrifuge if parts of its cover panels are damaged or missing.
•Do not touch the electronic components of the centrifuge or alter any electronic or
mechanical components.
•Please observe the safety instructions.
Please pay particular attention to the following aspects:
•Location: Well-ventilated environment, set-up on a level and rigid surface with
adequate load-bearing capacity.
•Rotor installation: Make sure the rotor is locked properly into place before operating the
centrifuge.
•Especially when working with corrosive samples (salt solutions, acids, bases), the
accessory parts and vessel have to be cleaned carefully.
•Always balance the samples.

PPrecautions
vi Cryofuge 5500i Thermo Scientific
Centrifuging hazardous substances:
WARNING
If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the
area immediately.
•Do not centrifuge explosive or flammable materials or substances which could react
violently with one another.
•The centrifuge is neither inert nor protected against explosion. Never use the centrifuge
in an explosion-prone environment.
•Do not centrifuge inflammable substances.
Remaining risk: Improper use can cause damages, contamination, and injuries
with fatal consequences.
•Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms
without suitable safety precautions.
When centrifuging microbiological samples from the Risk Group II (according to the
“Bio-safety Manual”of the World Health Organization (WHO)), aerosol-tight
biological seals have to be used.
For materials in a higher risk group, extra safety measures have to be taken.
•If toxins or pathogenic substances have gotten into the centrifuge or its parts,
appropriate disinfection measures have to be taken (see “Disinfection” on page 5-3).
Remaining risk: Improper use can cause damages, contamination, and injuries
with fatal consequences.
•Highly corrosive substances which can cause material damage and impair the
mechanical stability of the rotor, should only be centrifuged in corresponding protective
tubes.

Thermo Scientific Cryofuge 5500i 1-1
1
Introduction and Description
Contents
•“Characteristics of the Cryofuge 5500i” on page 1-2
•“Technical Data” on page 1-3
•“Directives, Standards and Guidelines” on page 1-4
•“Functions and Features” on page 1-4
•“Mains Supply” on page 1-5
•“Refrigerants” on page 1-6
•“Rotor Selection” on page 1-6

1Introduction and Description
Characteristics of the Cryofuge 5500i
1-2 Cryofuge 5500i Thermo Scientific
Characteristics of the Cryofuge 5500i
The Cryofuge 5500i is a large volume, refrigerated universal centrifuge.
The Cryofuge 5500i is designed for large volume, high throughput laboratory needs. The micro test
plates and tubes can be centrifuged with the unique double rectangular bucket.
The Cryofuge 5500i is optimized for the automatic laboratory processes in clinical central labs.
The Diagnostik™ rotor allows the centrifuge commercial sample racks directly.
The Cryofuge 5500i is also a compact centrifuge for the separation of blood components. A special
wind shield rotor allows to process up to eight quintuple blood bag systems.
The Cryofuge 5500i is equipped with various safety features:
•The housing and rotor chamber consist of steel plate, the interior of armor steel, while the front
panel is made of high-impact resistant plastic.
•The door is equipped with a view port and a lock.
•The door of the centrifuge can only be opened while the centrifuge is switched on and the rotor
has come to a complete stop. The centrifuge cannot be started until the door has been closed
properly.
•The integrated rotor detection systems ensure that no inadmissible speed settings can be
preselected.
•Electronic imbalance detection, SMARTspin.
•Door emergency release: For emergencies only, e.g. during power failures
(see “Mechanical Emergency Door Release” on page 6-2).

1 Introduction and Description
Technical Data
Thermo Scientific Cryofuge 5500i 1-3
Technical Data
The technical data of the Cryofuge 5500i is listed in the following table.
Table 1-1. Technical Data
Feature Value
230 V 200 V 120 V
Environmental Conditions -Use in interior spaces
-Altitudes of up to 2,000 m above sea level
-max. relative humidity 80 % up to 31 °C; decreasing linearly up to
50 % relative humidity at 40 °C.
Permissible Ambient Temperature +2 °C to +40 °C +2 °C to +40 °C +2 °C to +40 °C
Overvoltage Category II II II
Pollution Degree 2 2 2
Heat Dissipation 9200 BTU/h 9200 BTU/h 6300 BTU/h
IP 20 20 20
Running Time unlimited unlimited unlimited
Max Speed nmax 10000 rpm (depending
on the rotor)
10000 rpm (depending
on the rotor)
10000 rpm (depending
on the rotor)
Min Speed nmin 300 rpm 300 rpm 300 rpm
Maximum RCF Value at nmax 15317 x g15317 x g15317 x g
Maximum Kinetic Energy <63.4 kJ <63.4 kJ <63.4 kJ
Noise Level at maximum Speed <61 dB(A) <61 dB(A) <61 dB(A)
Temperature Setting Range -9 °C to +40 °C -9 °C to +40 °C -9 °C to +40 °C
Dimensions
Height
Width
Depth
960 mm
680 mm
720 mm
960 mm
680 mm
720 mm
960 mm
680 mm
720 mm
Weight without Rotor 286 kg 286 kg 286 kg
Environmental Conditions
during Storage and Shipping
Temperature:
-10 °C to +55 °C
Humidity: 15 % to 85 %
Temperature:
-10 °C to +55 °C
Humidity: 15 % to 85 %
Temperature:
-10 °C to +55 °C
Humidity: 15 % to 85 %

1Introduction and Description
Directives, Standards and Guidelines
1-4 Cryofuge 5500i Thermo Scientific
Directives, Standards and Guidelines
Functions and Features
The following table gives an overview of the important functional and performance characteristics of
the Cryofuge 5500i.
Table 1-2. Directives, Standards and Guidelines
Table 1-3. Functions and Features
Component / Function Description / Features
Structure / Housing Galvanized Steel Chassis with Armored Plating
Rotor Chamber Stainless Steel
Drive Induction Drive without Carbon Brushes
Keys and Display Easy-to-clean Keypad and Display Surface
Controls Microprocessor controlled with Easycontrol II
Internal Memory The most recent Data is saved
Functions RCF-Selection, Temperature Regulation during Standstill, Pre-Temp,
and PULSE
Acceleration / Braking Profiles 9 Acceleration and 9 Braking Curves
Rotor Recognition Automatic
Imbalance Detection SMARTspinElectronic, contingent on Rotor and Speed
Door Lock Motor assisted Door Looking
Region Standards and Guidelines
Europe 2006/42/EC Machinery
Protective goals of:
2014/35/EULow Voltage
2014/30/EU Electromagnetic
Compatibility (EMC)
2011/65/EU RoHS
Directive on the Restriction of
the use of certain Hazardous
Substances in electrical and
electronic equipment
EN 61010-1
EN 61010-2-020
EN 61326-1
USA & Canada cCSAus approved:
The centrifuges are produced and
inspected according to the
following standards and
guidelines
UL 61010-1
CAN/CSA-C22.2 No. 61010-1
IEC 61010-2-020
(Pollution degree 2, Overvoltage category II)
Directive

1 Introduction and Description
Mains Supply
Thermo Scientific Cryofuge 5500i 1-5
Mains Supply
Function Feature
Program Display
1...9
Free Program Selection
Operating Memory
Memory Capacity for Set Talues
PULSE-Mode
Acceleration / Braking Profiles 1 = slow, ... 9 = fast Acceleration / Deceleration Curve
Speed Selection Adjustable from 300 rpm to 10,000 rpm in 10 rpm Increments
RCF Selection After actuating the RCF Switch Key, the RCF Value can be entered.
Time Selection Adjustable in Seconds up to 9 min 59 sec,
in the Range of 10 min up to 99 min
in Minutes, "hold"-mode: Continuous Operation
Run Time Display in "quick run"
Mode
In Seconds Increments
Temperature Selection Adjustable from -9 °C to +40 °C, in 1 °C Increments.
End of Run The Speed Display will read End.
Door Opening Automatic unlocking via Key
(unlocking in Case of Power Failure: see chapter “Troubleshooting”)
Start Key
Stop Key
PULSE-Mode Pressing the Key activates maximum Acceleration up to the maximum
permissible Speed of Rotor; upon Key release Centrifuge stops with maximum
Deceleration Power.
Diagnostic Messages Alternating Display rotor / maximum Speed or RCF (Speed selected exceeds
max. Speed of the Rotor)
Door has not been lifted off the Lock during Opening: Display Lift Lid (manual
Lifting of door required)
General Centrifuge malfunction (Error Messages with ERROR Codes,
see “Troubleshooting by User”on page 6-3)
The following table contains an overview of the electrical connection data for the Cryofuge 5500i.
This data is to be taken into consideration when selecting the mains connection socket.
Table1-4.Electrical Connection Data
Cat. Mains
Voltage Frequency Rated Current
Power
Consumption
Equipment
Fuse
Building
Fuse
75004471 230 V 50 Hz 12 A 2.7 kW 16 AT 16 AT
75004475 120 V 60 Hz 16 A 1.8kW 16 AT 20 AT
75004479 200 V50 / 60 Hz 14.5 / 16 A2.7 kW 16 AT 20 AT
75004479 208V 60 Hz 14.5 A 2.7 kW 16 AT 20 AT
75004479 230 V 60 Hz 14.5 A 2.7 kW 16 AT 20 AT

1-6 Cryofuge 5500i Thermo Scientific
1Introduction and Description
Refrigerants
Refrigerants
The Cryofuge 5500i is supplied without a rotor.
Various Thermo Scientific rotors are available to choose from.
The technical data of the rotors and the corresponding adapters and reduction sleeves for various
commercially available containers can be found in the corresponding rotor operating manuals.
For more information visit our website at: http://www.thermofisher.com
LH-40001
1This rotor is not available for the 120 V centrifuge.
75006475
with round Buckets 1000 75006477
Double rectangular Bucket DoubleSpinTM 2 x 250 75006478
LH-4000W 75006476
with round Buckets 1000 75006477
Double rectangular Bucket DoubleSpinTM 2 x 250 75006478
with double Blood Bag Bucket2
2This rotor is not available for the 120 V centrifuge.
75006436
BIOshield 4 x 250 75006435
HIGHPlate 5 x 5 Platten 75006444
DiagnostikTM 75006480
HIGHConic6 x 50 75003057
LAC-250 6 x 250 75006483
Rotor Selection
Table1-5.Refrigerants
Article No.
75004471 Thermo Scientific Cryofuge 5500i
75004475
75004479
Centrifuge Refrigerant Quantity Pressure GWP CO2e
Thermo Scientific Cryofuge 5500i
Thermo Scientific Cryofuge 5500i
R-134A
R-134A
R-134A
1 kg 21 bar 1430 1.43 t
1 kg 21 bar 1430 1.43 t
1 kg 21 bar 1430 1.43 t
Contains fluorinated greenhouse gases in a hermetically sealed system.

Thermo Scientific Cryofuge 5500i 2-1
2
Before Use
Contents
•“Before Setting up” on page 2-2
•“Setting up” on page 2-2
•“Aligning the Centrifuge” on page 2-4
•“Transporting the Centrifuge” on page 2-4
•“Location”on page 2-4
•“Mains Connection”on page 2-5
•“Adjust Voltage and Frequency” on page 2-6
•“Storage” on page 2-6

2Before Use
Before Setting up
2-2 Cryofuge 5500i Thermo Scientific
Before Setting up
1. Check the centrifuge and the packaging for any shipping damage.
Inform the shipping company and Thermo Fisher Scientific immediately,if any damage
is discovered.
2. Remove the packaging.
3. Check the order for completeness (see “Scope of Supply” on page iii).
If the order is incomplete, please contact Thermo Fisher Scientific.
Setting up
1. Place the pallet with the centrifuge so that you have atleast2 m room in front of the pallet.
2. Release the rails from the pallet.
3. Screw the rails onto the pallet in front of the centrifuge.
WARNING The centrifuge operated with a high kinetic energy. Do not operate the
centrifuge without a 30 cm (12 inch) safety zone around the centrifuge.
Do not operate the centrifuge on its castors.
Note The centrifuge is screwed onto the pallet with four screws.
Rails
Screws

2 Before Use
Setting up
Thermo Scientific Cryofuge 5500i 2-3
4. Screw the threaded rods with the threaded nuts as far up as possible. Otherwise they might get
stuck when rolling the centrifuge off the pallet.
5. Remove the locking stabilizers.
6. Check that the steering casters point to the back.
7. Use several people and other means in order to roll the centrifuge off the pallet.
8. Once the centrifuge is in its final position mount the enclosed locking stabilizers.
9. Screw the threaded nuts down.
10. Place the locking stabilizers centrally under the threaded rods and align the centrifuge. All the
locking stabilizers should carry uniformly (check by a tilt test). The casters are some 5-15 mm off
the ground.
Threaded rod
Lock nut
Safety bracket
Locking stabilizer
Threaded nut
Threaded nut
WARNING Due to the centrifuges weight it must be controlled by several people when
rolling of the pallet. Do not stand in front of the centrifuge when rolling down for the
centrifuge might run you over.

2Before Use
Aligning the Centrifuge
2-4 Cryofuge 5500i Thermo Scientific
Aligning the Centrifuge
11. Switch on the centrifuge.
12. Open the centrifuge.
13. Put the enclosed water level on the drive spindle.
14. Use an open-end wrench to align the centrifuge and to raise and lower it.
15. Remove the water level when the centrifuge is leveled.
16. Be sure that the four locking stabilizers stand safe and do not jolt.
17. Fasten the lock nuts by screwing them up as far as possible.
18. Screw the safety bracket onto the locking stabilizers.
19. The final assembly of the safety brackets is carried out after leveling.
Transporting the Centrifuge
•Use a forklift to lift the centrifuge.
•The centrifuge can be damaged by impacts.
•Transport the centrifuge upright and if at all possible,in packaging.
Location
The centrifuge should only be operated indoors.
The set-up location must fulfill the following requirements:
•A safety zone of at least 30 cm must be maintained around the centrifuge.
People and hazardous substances must be kept out of the safety zone while centrifuging.
•The supporting structure must be stable and free of resonance.
•The supporting structure must be suitable for horizontal setup of the centrifuge.
CAUTION If the centrifuge is not level, imbalances can occur and the centrifuge can be
damaged.
The horizontal alignment of the centrifuge must be checked every time after moving it to
a different location.
The supporting structure must be suitable for horizontal setup of the centrifuge.
Do not place anything under the feet to level the centrifuge.
Note The packaging is a one-way packaging. Contact a shipping company for the transport.
Inform customer service.
WARNING Always remove the rotor before moving the centrifuge.

2 Before Use
Mains Connection
Thermo Scientific Cryofuge 5500i 2-5
•The centrifuge should not be exposed to heat and strong sunlight.
•The set-up location must be well-ventilated at all times.
Mains Connection
Figure 2-1. Mains Connection
1. Turn off the power supply switch located on right side (press “0”).
2. Plug the centrifuge into grounded electrical sockets only.
3. Check whether the cable complies with the safety standards of your country.
4. Make sure that the voltage and frequency correspond to the figures on the rating plate.
5. Establish the connection to the power supply with the connecting cable.
WARNING UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.
Mains plug

2Before Use
Adjust Voltage and Frequency
2-6 Cryofuge 5500i Thermo Scientific
Adjust Voltage and Frequency
It is necessary that you set the voltage and frequency during setup.
1. Pull out the power supply plug.
2. Set the switches on the back as shown in the table.
3. Connect the centrifuge to the power supply again.
Storage
•Before storing the centrifuge and the accessories it must be cleaned and if necessary,disinfected and
decontaminated.
•Store the centrifuge in a clean, dust-free location.
•Be sureto place the centrifuge on its feet.
•Avoid direct sunlight.
CAUTION Remove the power supply plug in order to prevent damage to the centrifuge.