ThermoFisher Scientific Applied Biosystems Veriti 4375305 User manual

For Research Use Only. Not for use in diagnostic procedures.
Veriti™Thermal Cycler
USER GUIDE
Catalog Numbers 4375305, 4375786, 4384638, 4388444, 4413751, 4413964, and ZGEXSCVERTI3Y
Publication Number MAN0018441
Revision B.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0018441
Revision Date Description
B.0 17 June 2021 Addition of information on Fleet Control with InstrumentConnect.
A.0 24 January 2020 Converted into CCMS. Removed sku N801537. Updated figure10.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2021 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER 1 System overview ..................................................... 8
Introducing the Veriti™Thermal Cycler ............................................. 8
About the instrument ........................................................ 8
Using the touchscreen ........................................................... 9
Entering text .............................................................. 10
Entering numerals ......................................................... 11
Menu overview ................................................................ 12
Main Menu screen ......................................................... 12
Settings Menu screen ...................................................... 13
Admin Menu screen ....................................................... 13
Tools Menu screen ........................................................ 14
About run methods ............................................................. 14
What is a run method? ..................................................... 14
Predefined methods ....................................................... 14
Viewing a run method ...................................................... 15
Managing run methods ..................................................... 15
Administrative tasks for run methods ......................................... 16
Copying a run method ...................................................... 17
Printing a run method ...................................................... 17
Deleting a run method ...................................................... 18
Designating a run method as the default ...................................... 18
Creating a shortcut to a run method .......................................... 19
Backing up run methods and other settings ................................... 19
Restoring run methods and other settings .................................... 21
About folders .................................................................. 22
Deleting custom folders .................................................... 22
Renaming custom folders ................................................... 22
■CHAPTER 2 Getting started ...................................................... 24
Installing the Veriti™Thermal Cycler .............................................. 24
Before you start ........................................................... 24
Environmental requirements ................................................. 25
Materials ................................................................. 25
Unpacking the instrument .................................................. 27
Setting up the instrument ................................................... 28
Connecting to a network printer ............................................. 28
Veriti™Thermal Cycler User Guide 3

Connecting multiple instruments ............................................. 29
Powering on .............................................................. 30
Setting up the Veriti™Thermal Cycler ............................................. 30
Recommended instrument settings .......................................... 30
Setting up instrument security and user accounts .............................. 33
Adding users .............................................................. 34
Setting other options ........................................................... 38
Setting idle temperatures for the block(s) and cover ............................ 39
Setting time-outs .......................................................... 39
Configuring main menu shortcuts to functions ................................. 40
About the Thermal Cycler Fleet Control Software ................................... 41
Connect the thermal cycler to the network .................................... 41
■CHAPTER 3 Performing a run .................................................... 42
Selecting disposables .......................................................... 42
Disposables for 96‑well instruments .......................................... 42
Disposables for the 384-well instrument ...................................... 44
Disposables for the 60-well Veriti™Thermal Cycler ............................. 44
Loading samples into the instrument ............................................. 44
Loading Samples in 96-well instruments ...................................... 45
Loading samples in the 384-well instrument ................................... 46
Loading samples in the Veriti™60-well Thermal Cycler .......................... 47
Creating a new run method ...................................................... 47
Editing details ................................................................. 49
Adding a stage ............................................................ 49
Adding a step ............................................................. 50
Editing temperature and time ................................................ 51
Editing ramp rates ......................................................... 52
Creating an AutoDelta step ................................................. 52
Adding a pause ............................................................ 54
Deleting a stage or step .................................................... 55
Creating a VeriFlex™step ................................................... 55
Performing a run ............................................................... 57
Before you begin .......................................................... 57
Monitoring a run ............................................................... 59
Run Monitor screen ........................................................ 59
Actions in the Run Monitor screen during a run ................................ 60
Changing the number of cycles .............................................. 61
Pausing a run ............................................................. 61
Stopping a run ............................................................ 62
Using the Run Monitor button ............................................... 62
If the power fails ........................................................... 63
Viewing and printing the run report ............................................... 63
Removing the samples from the instrument ....................................... 64
Contents
4Veriti™Thermal Cycler User Guide

■CHAPTER 4 Utilities ............................................................... 65
Using GeneAmp™PCR System 9700 run methods ................................. 65
Upgrading system firmware ..................................................... 67
About the upgrade ......................................................... 67
Upgrading the instrument firmware .......................................... 67
Calculating melting temperatures ................................................ 69
Viewing instrument statistics .................................................... 70
Restoring the factory settings .................................................... 70
Running the cycle performance test .............................................. 71
Required materials ......................................................... 72
Procedure ................................................................ 72
Tests using the temperature verification kit ........................................ 72
About the temperature verification kit ......................................... 73
For more information ....................................................... 73
Running the heated cover verification ........................................ 73
Running the temperature verification ......................................... 74
Running the temperature nonuniformity test ................................... 75
TVK recalibration .......................................................... 76
■CHAPTER 5 Maintenance ......................................................... 77
Scheduled maintenance ........................................................ 77
As-Needed maintenance ........................................................ 77
Tests ..................................................................... 77
Cleaning the instrument .................................................... 77
Replacing fuses ........................................................... 80
Parts list ...................................................................... 81
■APPENDIX A Troubleshooting .................................................... 83
■APPENDIX B Returning an instrument for service .............................. 85
■APPENDIX C Disposables ........................................................ 86
Disposables ................................................................... 86
Other supplies for sample handling ............................................... 88
■APPENDIX D Instrument specifications ......................................... 89
System specifications .......................................................... 89
Physical dimensions and electrical specifications .............................. 89
Power and communication connections ...................................... 89
Sample temperature range .................................................. 90
Contents
Veriti™Thermal Cycler User Guide 5

Touchscreen specifications ................................................. 90
Sample well specifications .................................................. 90
Printer specifications ........................................................... 91
■APPENDIX E Predefined run methods ........................................... 92
Predefined run methods ........................................................ 92
AmpliCycle™Seq Run Method .............................................. 93
AmpliTaq Gold™DNA Polymerase Run Method ................................ 93
AmpliTaq Gold™Fast PCR Run Method ....................................... 93
BigDye™Kit Fast Run Method ............................................... 94
BigDye™Kit Standard Run Method ........................................... 94
Fast PCR Run Method ...................................................... 94
General PCR Run Method .................................................. 95
LMS2 Run Method ......................................................... 95
Time Release Run Method .................................................. 95
Touchdown PCR Run Method ............................................... 96
XL PCR Run Method ....................................................... 96
Limited product warranty ........................................................ 96
■APPENDIX F Safety and EMC compliance information ........................ 97
Safety conventions used in this document ........................................ 97
Safety alert words ......................................................... 97
Symbols on instruments ........................................................ 98
Electrical symbols on instruments ........................................... 98
Environmental symbols on instruments ....................................... 98
Safety symbols ............................................................ 99
Safety labels on instruments ................................................ 99
General instrument safety ...................................................... 100
Moving and lifting the instrument ........................................... 100
Operating the instrument .................................................. 100
Cleaning or decontaminating the instrument ................................. 101
Electrical safety ............................................................... 101
Fuses ................................................................... 101
Power ................................................................... 101
Overvoltage rating ........................................................ 101
Safety and electromagnetic compatibility (EMC) standards ......................... 102
Physical hazard safety ......................................................... 102
Moving parts ............................................................. 102
Chemical safety .............................................................. 103
Biological hazard safety ....................................................... 104
Contents
6Veriti™Thermal Cycler User Guide

■APPENDIX G Documentation and support .................................... 105
Customer and technical support ................................................ 105
Limited product warranty ...................................................... 105
Contents
Veriti™Thermal Cycler User Guide 7

System overview
Introducing the Veriti™Thermal Cycler
About the instrument
There are four models of the Veriti™Thermal Cycler:
•Veriti™96-Well Fast Thermal Cycler (PN 4375305), with 0.1–mL sample wells
•Veriti™96-Well Thermal Cycler (PN 4375786), with 0.2–mL sample wells
•Veriti™384-Well Thermal Cycler (PN 4388444), with 0.02–mL sample wells
•Veriti™60-Well Thermal Cycler (PN 4384638), with 0.5–mL sample wells
The Veriti™Thermal Cycler is controlled using a touchscreen display with easy-to-use interface. It is
also compatible with the Applied Biosystems™Thermal Cycler Fleet Control Software. Up to twelve
Veriti™Thermal Cyclers can be connected in a satellite-like format through a network switch. With this
connection, you can transmit run methods from one Veriti™thermal cycler to another and start multiple
thermal cyclers at once.
The Veriti™Thermal Cycler has a run recovery feature. If a power failure occurs during a run, the
instrument will attempt to continue the run when the power returns.
In addition, the Veriti™96-Well Thermal Cyclers have VeriFlex™Blocks™. These are six independently
regulated thermal blocks to aid in designing a primer set and run method for optimal PCR conditions.
Figure 1 The Applied Biosystems™Veriti™thermal cycler
This user guide provides instructions for all four models of the Veriti™Thermal Cycler. Functions which
are not the same for each model are indicated by a comment such as “This function is only available for
the Veriti™96-Well Thermal Cyclers”.
See Appendix D, “Instrument specifications” for detailed specifications of the Veriti™Thermal Cycler.
1
8Veriti™Thermal Cycler User Guide

Using the touchscreen
You interact with the instrument using a touchscreen.
Table 1 describes several buttons common to many of the touchscreens.
Table 1 Buttons in the instrument touchscreen
Button Function
Returns to the Main Menu screen.
Returns to the previous screen.
When a run is in progress, displays the Status Report to view any errors that occurred
during the run.
When a run is in progress, displays the Run Monitor screen to view the run.
Scrolls up and down through a list of items.
Displays the run method folders.
Creates a “shortcut” to the selected run method (or methods).
Indicates that a USB drive is connected to the instrument’s USB port.
Opens the Help system.
Chapter 1 System overview
Using the touchscreen 1
Veriti™Thermal Cycler User Guide 9

Entering text
When you touch a field that requires the input of text, the text editor opens (Figure 2).
Figure 2 The instrument text editor
To… Select…
Enter a lowercase letter A letter button.
Enter an uppercase letter
Caps, then the letter(s).
When you are done, select Caps again.
Enter punctuation or other symbols
Symbols then the symbol(s).
When you are done, select Symbols again.
Remove the contents of the entire field
Delete the last letter
Close the editor and save any changes
Chapter 1 System overview
Using the touchscreen
1
10 Veriti™Thermal Cycler User Guide