Biotage Extrahera Classic User guide

Biotage®Extrahera™
Installation and Safety

Biotage®Extrahera™Installation and Safety | © Biotage 2020
Contents
CONTENTS
Installation
1 Download Biotage®Extrahera™User Documentation
1 Software License Agreement
1 Delivered Items
1 Site Requirements
2 Move the System
5 Connections
5 Upgrade the Biotage®Extrahera™Software
Technical Specifications
6 System Requirements
6 Liquid Handling
6 Interfaces
7 Clog Detection
Safety
8 Intended Use
8 Education, Training, and Competence
8 Warranty and Liability
8 Service
8 Safety Features
8 Labels
9 Explosion Risk
9 Safety Requirements
11 Restriction of Hazardous Substances (RoHS) Directive
11 WEEE Compliance Statement
11 Safety in Other Languages
 General Information
12 Consumables and Accessories
12 Manufacturer
12 Contact Us
Biotage®Extrahera™
Installation and Safety


Installation
Site Requirements
Before the system is installed by an authorized Biotage service
engineer, the installation site should be prepared as follows:
Fume hood/
ventilation
system
The system must be either connected to a
ventilation system or placed in a well-ventilated
fume hood or an equivalent enclosure to reduce the
risk of exposure to harmful gases. The ventilation
system or fume hood must be capable of exhausting
6 cubic meters/min (212 cubic feet/min).
If the system is to be installed on a benchtop
(outside a fume hood or equivalent enclosure),
an air duct adapter supplied with the system must
be installed on the top of the ventilation fan to allow
connection to suitably sized ventilation tubing.
The outer diameter of the air duct adapter is
159.0 ± 0.3 mm (6.26" ± 0.01") and the inner is
152.7 ± 0.3 mm (6.01" ± 0.01"), i.e. the 160 mm
tube should be mounted outside the air duct adapter
and the 6" tube on the inside of the air duct adapter.
Weight and
dimensions
The total weight of the package including the
system is 120 kg (265 lbs). Use suitable lifting
equipment when moving the package. The system
is supplied on pallet and the dimensions of the
package (+ pallet) are: 1230 x 840 x 800 mm
(48.4" x 33.1" x 31.5").
The fume hood or bench must be able to support
the weight of the system, i.e. 75 kg (165 lbs).
The dimensions of the system are (W x D x H):
Without touch screen: 610 x 510 x 730 mm
(24.0" x 22.1" x 28.7")
With touch screen: 860 x 570 x 730 mm
(33.8" x 22.4" x 28.7")
Operating
temperature
18°C to 32°C
Ambient air The system must be located in an area where the
ambient air is clean. No emission of solid particles
or smoke in the air by adjacent equipment is
allowed. The level of dust should be comparable to
that of normal laboratory spaces.
Humidity: 10% to 90% RH
Electrical
supply
100 to 240 VAC, 50/60 Hz, 300 VA
Connect only to a properly grounded outlet.
Vacuum
supply
Partial pressure range: < 500 mbar (50 kPa; 7 psi)
Flow rate: ≥ 8.3 L/min (0.29 cubic feet/min)
Connection: The vacuum tube supplied with the
system has a 6 mm (0.24") outer diameter and
4 mm (0.16") inner diameter.
Warning
» The system must be unpacked and installed by an authorized
Biotage service engineer.
» Follow regional safety practices when handling and moving
shipping boxes and containers, and moving the system.
» Observe general and specific safety regulations for the use of
the system and its accessories and consumables at all times,
in order to reduce the risk of personal injury, fire, and
explosion; see "Safety Requirements" on page 9.
Download Biotage®Extrahera™
User Documentation
The following user documentation is supplied with the system
and can be downloaded at www.biotage.com:
» Biotage®Extrahera™Installation and
Safety, P/N 414157 (this document)
» Biotage®Extrahera™User Manual, P/N 414158
» Biotage®Extrahera™Safety Translations, P/N 414159
If you have problems downloading the user documentation,
please contact your local Biotage representative. See contact
information on the back of this document or visit our website
www.biotage.com.
Software License Agreement
Biotage Sweden AB licenses the Biotage®Extrahera software to
you only upon the acceptance of all of the terms and conditions
in the software license agreement.
By using the software, you consent to be bound by and are
becoming a party to that agreement.
To read the software license agreement, either request a copy
from your local Biotage representative before the installation or
read it on your Biotage®Extrahera system by pressing Licenses
in the software's About view.
Delivered Items
Note: We recommend that the boxes and packing materials are
kept by the customer in case the system needs to be returned
for service or moved to another location.
Check the delivery against the shipping documentation to ensure
that all parts are included in the shipment. If any part of the order
is missing or damaged, please contact
Biotage®1-Point Support™.
Installation

Biotage®Extrahera™Installation and Safety | © Biotage 2020
Installation
Positive
pressure unit
The gas applied to the samples (i.e. the gas
connected to the N2port) must be according to
ISO 8573-1, classification 1-6-1. This is essential to
prevent sample contamination and general fouling
of the pressure unit. The filter unit that is supplied
with the system delivers according to ISO 8573-1,
class 1-x-1. If the gas applied is not according
to class x-6-x, an air dryer needs to be ordered
separately (P/N 416441SP).
AIR port requirements: 5 L/min (0.18 cubic feet/
min), 6 ±0.2 bar (0.6 ±0.02 MPa; 87 ±3 psi) for a
fully populated extraction plate/rack and lower to
approximately 4 bar (0.4 MPa; 58 psi) when 50%
of the plate/rack is populated and 3 bar (0.3 MPa;
44 psi) when 25% of the plate/rack is populated.
N2 port requirements: 1 L/min (0.04 cubic
feet/min) during processing and 70 L/min
(2.5 cubic feet/min) during plate dry, 6 ± 0.2 bar
(0.6 ± 0.02 MPa, 87 ± 3 psi)
Connection: The gas tube supplied with the
system has a 6 mm (0.24") outer diameter and
4 mm (0.16") inner diameter.
External fire
protection
External fire protection should be installed
according to local regulations for equipment
operating unattended.
Move the System
Warning
» Before moving the system, please read and observe the safety
requirements in "Safety Requirements" on page 9.
If you need to move the system within the laboratory or between
laboratories in the same building, follow the instructions below.
If you need to ship the system, please contact Biotage®1-Point
Support™for instructions.
1. Prepare the new site according to the site requirements
on page 1.
2. Clean the waste tubing as described in the user manual
(P/N 414158).
3. Empty the system of liquids by flushing it with air:
a. Remove the solvent inlet lines from their bottles and
place them in an empty, clean bottle.
b. Ensure that you have five empty solvent reservoirs in
the solvent rack in position 5on the working area.
c. Press Maintenance in the main menu.
d. Press Flush Solvent Inlets….
e. Enter the flush volume for S1. 25 mL is required to
empty the solvent inlet line and pump of liquid.
f. Press Flush for S1.
g. When you have finished flushing the solvent inlet line
with air, repeat steps e to f for the other solvent inlet
lines (S2-S5).
4. Remove the solvent racks in position 5 and 6 (if used) on the
working area and empty the reservoirs.
5. Remove all accessories and consumables inside the system,
i.e. the pipette tip waste bin, plates, racks, etc.
6. Turn off the vacuum pump or, if your system is connected to
another vacuum source, close the valve.
7. Shut down the system by pressing Shut Down in the main
menu or login view (GLP) and then Yes to confirm.
8. When the message saying that it is safe to turn off the system
appears on the screen, turn off the system. The mains switch
is located on the right hand side at the rear of the system.
9. Unplug the power cord from the power outlet.
10. Turn off the gas supply/supplies, and disconnect the
pressure regulators and the gas tubes from 1) the supply/
supplies and 2) the AIR and N2ports located on the right
hand side at the rear of the system.
11. Disconnect the waste outlet tube from the waste reservoir
and empty the waste reservoir.
12. Clean the interior of the system and the accessories as
described in the user manual (P/N 414158).
13. Clean the touch screen and the exterior of the system,
using a soft and clean cloth. The cloth can be dry or lightly
dampened with a neutral detergent or alcohol.
14. If you need to remove the touch screen (computer):
a. Adjust the ball mount angle so that the rear of the touch
screen can be accessed; see Figure 1.
b. Remove the lower panel by unscrewing the six screws
using a Philips #2 screwdriver.
c. Disconnect the power connector by unscrewing its
locking ring.
d. Disconnect the two Ethernet cables and any USB
devices.
e. Remove the touch screen by loosening the ball mount.
Note: The touch screen must be placed on a flat and level
surface to avoid damage.
Figure 1.The rear of the touch screen (computer).


Installation
15. Attach the four lifting handles to the system; see Figure 2.
The handles are supplied with the system.
Figure 2.The four lifting handles supplied with the system must be used
when moving the system.
16. The system weighs 75 kg (165 lbs). Carefully lift the system
using the four lifting handles and place it on a trolley that
can support the weight of the system. Four persons are
required when lifting the system.
17. Move the trolley and the rest of the equipment (the pipette
tip waste bin, waste reservoir, vacuum pump (if used),
solvent bottles, etc.) to the new location.
18. Carefully lift the system and place it in a well-ventilated
fume hood or on a bench. Four persons are required when
lifting the system. Place the system so that the mains switch
is easy to access.
19. If the system is not placed in a fume hood, connect the outlet
of the Extrahera top ventilation to a ventilation system:
a. Connect an air duct adapter from Biotage to the outlet of
the Extrahera top ventilation.
b. Connect a ventilation tube between the air duct adapter
and the exhaust. The outer diameter of the air duct
adapter is 159.0 ± 0.3 mm (6.26" ± 0.01") and the inner
is 152.7 ± 0.3 mm (6.01" ± 0.01"), i.e. the 160 mm tube
should be mounted outside the air duct adapter and the
6" tube on the inside of the air duct adapter. Ensure that
the tube cannot be blocked.
20. It is important that the system is not tilting backward.
Level the system by adjusting the height of the feet. There is
a built-in spirit level in the bottom of the system, in the front
left corner. See Figure 3.
Figure 3.The built-in spirit level in the bottom of the system, in the front
left corner.
21. Place the solvent bottles on the side of the system and
insert the solvent inlet lines into the correct bottles.
Ensure to use appropriate caps to prevent harmful solvent
vapors from escaping and the contents from being spilled.
22. Place the waste reservoir on the side of the system and
connect the waste outlet tube and vacuum to it; see Figure 4.
If using a vacuum pump, ensure that the vacuum pump
fumes are directed into a proper ventilation system.
Figure 4
Connect to the upper port of the
waste valve, which is located on
the left side of your system
Connect to
the vacuum
source
Approx. 7 cm
.The setup of the waste kit.
23. Before connecting air and/or nitrogen to the system,
the pressure head must be removed to protect it from
contamination:
a. Remove the two screws holding the pressure head to the
pressure unit; see Figure 5.
Figure 5.The two screws attaching the pressure head to the pressure unit.
b. Remove the pressure head by pulling it down and then
pulling it out.
Note: If you have a system with only one gas tube
connected to the pressure head, you have to tilt the
pressure head horizontally to be able to pull it out.
c. Disconnect the gas tubes (or tube) from the pressure
head by pushing in the collar of each connector against
the fitting and pulling the tubing out; see Figure 6.
d. Put the pressure head on a clean and lint-free surface.
Figure 6.System with two gas tubes connected to the pressure head.

Biotage®Extrahera™Installation and Safety | © Biotage 2020
Installation
24. Connect a pressurized air or nitrogen supply to the AIR port
located on the right hand side at the rear of the system using
the pressure regulator as shown in Figure 7.
The gas connected to the AIR port is used to seal the plate/
columns. The pressure should be adjusted according to how
many positions in the extraction plate or column rack that
are populated. Use 6 ± 0.2 bar (0.6 ± 0.02 MPa; 87 ± 3 psi)
for a fully populated plate/rack and lower to approximately
4 bar (0.4 MPa; 58 psi) when 50% of the plate/rack is
populated and 3 bar (0.3 MPa; 44 psi) when 25% of the
plate/rack is populated.
Figure 7
Connect to
the AIR port
Connect to
supply
.The setup of the pressure regulator for the AIR port.
25. Connect a pressurized air or nitrogen supply to the N2port
located on the right hand side at the rear of the system using
the filter unit with a pressure regulator; see Figure 8. Set the
pressure to 6 ± 0.2 bar (0.6 ± 0.02 MPa; 87 ± 3 psi).
Figure 8
Connect to
the N2port
Connect
to supply
.The setup of the filter unit for the N2 port.
26. If you have the Extrahera GLP software and you want to
connect the system to your network, connect a network
cable between the LAN port located on the right hand side
of the system and your network.
27. If you have disconnected the touch screen, reconnect it by
reversing the instructions in step 14.
Note: Do not mix up the Ethernet cables. Connect according
to the labeling on the cables and the ports (LAN 1 and LAN 2).
28. Connect the system to a properly grounded (earthed) power
outlet with the correct mains voltage and frequency.
29. Ensure that the power cord and any cables, hoses, and
tubing connected to the system cannot come in contact with
chemicals. Corrosives and solvents can degrade the cord/
cable insulation and dissolve the hoses and tubing. There is
a risk of electric shock, fire, and/or equipment damage.
30. Turn on the system. The mains switch is located on the right
hand side at the rear of the system.
31. Purge the system:
a. Press Maintenance in the software's main menu.
b. Press Manual Control...and then Close in the Door field.
c. Press Move Out in the Pressure Unit field.
d. Enter 0.5 bar in the Pressure (bar) text box; see Figure 9.
e. Press Apply, wait for 15 seconds and then press Stop.
f. Press Move In in the Pressure Unit field.
g. Press Open Door.
Figure 9.The Manual Control view.
32. Reconnect the gas tubes to the pressure head. Ensure that
they are properly fastened by pulling on them and that they
are on the left side of the bracket as shown in Figure 10.
Figure 10.Ensure that the gas tubes are on the left side of the highlighted
bracket.
33. Put the pressure head back in place using the two screws.
Do not overtighten the screws.


Installation
34. Place the pipette tip waste bin for used pipette tips below
the WASTE position on the working area.
35. Place the solvent rack with five new, empty solvent reservoirs
in position 5 on the working area. Ensure that the solvent
feeder is pulled out into the correct position; see Figure 11.
Figure 11.The solvent feeder in position.
Connections
Figure 12
1 2 3 4 5
.Solvent connections located on the back wall inside the system.
Port Label Connect
1S1 The S1 solvent inlet line (right port) and the
S1 solvent feeder tube (left port).
2S2 The S2 solvent inlet line (right port) and the
S2 solvent feeder tube (left port).
3S3 The S3 solvent inlet line (right port) and the
S3 solvent feeder tube (left port).
4S4 The S4 solvent inlet line (right port) and the
S4 solvent feeder tube (left port).
5S5 The S5 solvent inlet line (right port) and the
S5 solvent feeder tube (left port).
Figure 13
6
7
8
.Waste connections located on the back wall inside the system.
Port Label Connect
6Waste outlet tube between the waste valve
and the waste reservoir.
7 + 8 Waste tube between the waste valve and
the extraction waste collector.
Figure 14
10
9
12
11
.Connections located on the right hand side at the rear of the
system.
Port Label Connect
9T4A-250VAC Electrical supply: 100 to 240 V~, 50/60 Hz,
300 VA
Fuses: T4A (2 required)
10 LAN Network. Optional.
11 N2The gas applied to the samples through
the pressure head. See the gas supply
requirements in the technical specification
on page 6.
12 AIR The gas used for the movement of the
pressure head. See the gas supply
requirements in the technical specification
on page 6.
Upgrade the Biotage®Extrahera™Software
Please refer to the instructions delivered with the new software.

Biotage®Extrahera™Installation and Safety | © Biotage 2020
Technical Specifications
Note: The system contains corrosion sensitive parts.
Best practice is to avoid sustained continuous exposure
to acidic and basic vapors by always removing the solvent
reservoirs when the system is not in use, and cleaning the
system following usage. Usage of strong acids (e.g. TFA and
TCA) and inorganic acids (e.g. nitric, sulfuric, hydrochloric acid,
and perchloric acids) in the solvent pumps is not supported
at any concentration.
System Requirements
Ambient
temperature
Operating temperature: 18°C to 32°C
Storage and transportation temperature:
-25°C to 60°C
Humidity 10% to 90% RH
Electrical supply 100 to 240 VAC, 50/60 Hz
Fuses T4A at the power inlet (2 required)
Maximum power
consumed
300 VA
Gas supply The gas applied to the samples (i.e. the gas
connected to the N2port) must be according
to ISO 8573-1, classification 1-6-1. The filter
unit that is supplied with the system delivers
according to ISO 8573-1, class 1-x-1. If the
gas applied is not according to class x-6-x,
an air dryer needs to be ordered separately
(P/N 4164 41SP).
AIR port: 5 L/min (0.18 cubic feet/min),
6 ±0.2 bar (0.6 ±0.02 MPa; 87 ±3 psi) for
a fully populated extraction plate/rack and
lower to approximately 4 bar (0.4 MPa; 58 psi)
when 50% of the plate/rack is populated and
3 bar (0.3 MPa; 44 psi) when 25% of the
plate/rack is populated.
N2 port: 1 L/min (0.04 cubic feet/min)
during processing and 70 L/min (2.5 cubic
feet/min) during plate dry, 6 ± 0.2 bar
(0.6 ± 0.02 MPa, 87 ± 3 psi)
Vacuum supply Partial pressure range: < 500 mbar (50 kPa;
7 psi)
Flow rate: ≥ 8.3 L/min (0.29 cubic feet/min)
Weight 75 kg (165 lbs)
Dimensions
(WxDxH)
Without touch screen: 610 x 510 x 730 mm
(24.0" x 22.1" x 28.7")
With touch screen: 860 x 570 x 730 mm
(33.8" x 22.4" x 28.7")
Max sound level 65 dB(A)
Liquid Handling
Solvent inlets 5
Solvents are pumped into five 25 mL reservoirs
inside the system using the five solvent pumps.
It is possible to have an extra solvent rack with
five 25 mL or 100 mL reservoirs. These have to
be filled manually.
The dead volume is 4 mL for both the 25 and
100 mL reservoir, and the 100 mL reservoir has
a maximum fill volume of 90 mL.
Waste outlet 1
Pipetting pump 50 to 1000 µL
At 50 µL: ±2.0% accuracy and 1.0% CV
At 100 µL: ±2.0% accuracy and 1.0% CV
At 500 µL: ±1.5% accuracy and 1.0% CV
At 1000 µ: ±1.0% accuracy and 1.0% CV
Processing
format
96-well extraction plates, 96-array plates for
1 and 2 mL wells, 96-position extraction racks
for 1 mL (tabless) columns (A format), 48-well
extraction plates, 48-position extraction racks
for 3 mL (tabless) columns (B format), and
24-position extraction racks for 1, 3, and 6 mL
(tabless) columns (A, B, and C format)
Processing
pressure
0 to 5 bar
Interfaces
Touch screen 12.1"
Ethernet LAN Complies with IEEE 802.3 (ANSI 8802-3)
USB USB 2.0
Technical Specifications


Technical Specifications
Clog Detection
With an Extrahera GLP software license installed on your system
(sold separately), clog detection is available for load, wash, and
elute operations in the SPE, SLE, and Filtration+ methods.
Supported Solvents
Solvent/Solvent Combination Clog Detection Compatibility
Acetone Good
Acetonitrile Excellent
Chloroform Poor
Dichloromethane Poor
Diethyl ether Poor
Dimethylformamide (DMF) Excellent
Dimethylsulfoxide (DMSO) Excellent
Ethanol Excellent
Ethyl acetate Good
Heptane Good
Hexane Poor
Isooctane Good
Isopropanol Excellent
Methanol Excellent
Pentane Poor
Tetrahydrofuran (THF) Poor
Toluene Excellent
Water Excellent
Methyl tert-butyl ether (MTBE) Poor
IPA 30% Excellent
Ammonium hydroxide 5% Excellent
Formic acid 4.6M Excellent
DCM/IPA, [90:10] Poor
DCM/IPA, [95:5] Poor
DCM/IPA/NH4OH [78:20:2] Poor
DCM/MeOH/NH4OH [78:20:2] Poor
MeOH/NH4OH [95:5] Excellent
Currently Not Supported Consumables
The following Biotage and competitor consumables are not
supported when using clog detection:
» ISOLUTE®PPT+
» ISOLUTE®PLD+
» ISOLUTE®FILTER+ Plate
» Waters Oasis Elution Plates
» Tecan NBE and Cerex Columns
» 1 mL Columns, 24 Positions Configuration
» 1 mL Columns, 96 Positions Configuration

Biotage®Extrahera™Installation and Safety | © Biotage 2020
Safety
Intended Use
The Biotage®Extrahera™system from Biotage is intended solely
for automating sample preparation. The system has to be
operated in a laboratory environment by trained professionals.
All operations must be performed:
» According to the user documentation
delivered with the system.
» According to instructions available at www.biotage.com.
» According to instructions provided through
dialogs appearing on the screen.
» According to instructions given by the
technical support staff from Biotage.
» Within limits set by the system’s technical specification.
Failure to follow those instructions and operate within the limits
set by the technical specification may result in personal injury
and/or equipment damage.
Education, Training, and Competence
It is your responsibility to provide all applicable health and
safety regulations to your personnel. You must also ensure that
all personnel involved in the operation and maintenance of the
system fulfill the following criteria:
» Have the necessary education, training, and competence
required for the intended use of the system.
» Observe general and specific safety regulations
for the use of the system and its accessories and
consumables at all times, in order to reduce the
risk of personal injury, fire, and explosion.
Warranty and Liability
See the “Biotage Terms & Conditions of Sale” document at
www.biotage.com.
Service
All service must be performed by an authorized Biotage service
engineer. Before handing over the system for service, it should
be emptied of liquid and cleaned from harmful residues.
It is the responsibility of the customer to inform Biotage 1-Point
Support representatives if the system has been used with
hazardous biological, radioactive, or toxic samples and/or
solvents, prior to any service being performed. When returning
equipment to Biotage, this should be done in accordance with
the material return procedures supplied separately by Biotage.
Only genuine Biotage accessories must be used in the system.
Safety Features
The ventilated system enclosure protects the user against
mechanical hazards and potentially harmful solvents and/or
vapors. The system cannot be operated when:
» the door is open, and/or
» the integral system ventilation fan is not working.
Labels
Labels used on the system:
In accordance with all the essential requirements of
all applicable European product directives; see the
Declaration of Conformity document supplied with
the product.
In accordance with both U.S. and Canadian safety
standards; see the Declaration of Conformity document
supplied with the product.
RoHS In accordance with the Restriction of Hazardous
Substances Directive; see "Restriction of Hazardous
Substances (RoHS) Directive" on page 11 and the
Declaration of Conformity document.
The product contains certain hazardous substances and
can be used safely during its environmental protection
use period (EPUP), as indicated by the number in the
center, and should enter into the recycling system after
the EPUP.
Subject to the Waste Electrical and Electronic
Equipment (WEEE) Directive; see "Restriction of
Hazardous Substances (RoHS) Directive" on page 11.
Manufacturer.
Consult accompanying user documentation.
The samples used with the system are potentially
hazardous and can cause illness.
Observe general as well as specific safety regulations
for the use of the system and its accessories and
consumables at all times, in order to reduce the
risk of personal injury; see "Safety Requirements"
on page 9.
There is a risk of crushing when the system is in
service mode. Note that all service must be performed
by an authorized Biotage service engineer.
Safety


Safety
Explosion Risk
The system has open solvent reservoirs. If the ventilation fails
and solvent vapors are not removed, an explosive environment
could be generated. If the system is found with the door closed
and the power off, you must:
1. Ventilate the system properly by opening the door manually
using the T25 Torx screwdriver supplied with the system; see
Figure 15 below. Ensure to take the necessary precautions to
avoid exposure to potentially harmful solvents and/or vapors.
2. Remove all solvent reservoirs and remove any spillage
before turning the system back on.
We do not recommend that the system is left unattended for an
extended period of time when using flammable solvents.
Figure 15.Open the door manually by turning the screw counterclockwise.
Safety Requirements
You must observe all safety requirements when installing and
operating the system. Failure to install or use the system in
a manner specified by Biotage may result in personal injury
and/or equipment damage. If the system has been damaged or
does not function properly, shut it down, remove any solvents,
samples, and waste inside the system, and contact Biotage
1-Point Support immediately (www.biotage.com).
Installation
» The system must be unpacked and installed by an
authorized Biotage service engineer. Prepare the
installation site as described on page 1.
» Follow regional safety practices when handling and moving
shipping boxes and containers, and moving the system.
» The total weight of the package including the
system is 120 kg (265 lbs). Use suitable lifting
equipment when moving the package.
» The system weighs 75 kg (165 lbs). The four lifting handles
supplied with the system must be used when moving the
system. Four persons are required when lifting the system.
» Do not place any equipment or bottles on top of the system.
» The system must be electrically grounded (earthed).
Use only a power cord supplied by Biotage. The ground
prong on the cord plug must not be removed and the
plug should only be connected to a grounded outlet
as per local and national regulations. Keep the mains
plug easily accessible in case the system needs to
be disconnected quickly from mains power.
» Ensure that the power cord and any cables, hoses,
and tubing connected to the system cannot come
in contact with chemicals. Corrosives and solvents
can degrade the cord/cable insulation and dissolve
the hoses and tubing. There is a risk of electric
shock, fire, and/or equipment damage.
» The system must be either connected to a ventilation system
or placed in a well-ventilated fume hood or an equivalent
enclosure to reduce the risk of exposure to harmful gases.
The ventilation system or fume hood must be capable of
exhausting 6 cubic meters/min (212 cubic feet/min).
» When installing the system inside a fume hood, follow
local and national safety regulations for installing a
system inside a fume hood and the safety regulations
supplied by the fume hood manufacturer.
» When the system is connected to a ventilation system, an
air duct adapter from Biotage must be mounted between the
outlet of the Extrahera top ventilation and the ventilation
tube. Ensure that the ventilation tube cannot be blocked.
» If using a vacuum pump, ensure that the fumes
are directed into a proper ventilation system.

 Biotage®Extrahera™Installation and Safety | © Biotage 2020
Safety
Operation
» Use the system only for its intended purpose, as described
in the user documentation delivered with the system and
user documentation available at www.biotage.com.
» To avoid leakage, check the following
before operating the system:
» Ensure that all connections are properly connected and
tightened (see the "Connections" section on page 5).
» Ensure that the extraction waste collector, waste
reservoir, and solvent rack with five reservoirs (in
position 5 on the working area) are in place, and that the
waste reservoir and the pipette tip waste bin are not full.
» Ensure that pipette tips of the correct size have been
loaded into the correct positions in the pipette tip racks.
» To avoid damaging the system, use care to ensure that all
solvents used with the system are free of particulates.
» Never operate the system when damaged.
Chemical and Biological Safety
» Check regularly for leaks and spills. If leakage is
observed, follow the instructions in the “Troubleshooting”
section in the “Biotage®Extrahera™User Manual”
document. If spillage has occurred, follow the
instructions for cleaning the interior of the system in
the “Biotage®Extrahera™User Manual” document.
» Always place the solvent bottles and waste
reservoir on the side of the system.
» Use appropriate caps on the solvent bottles to
prevent harmful solvent vapors from escaping
and the contents from being spilled.
» The system operates using electricity, which can
introduce additional hazards with certain solvents
if not properly connected, vented, or set up with
recommended manufacturer approved settings.
» Never leave solvents, samples, or waste inside
the system when the ventilation is turned off.
» Follow all generally-accepted lab safety procedures
and applicable laws and regulations.
» All samples and waste should be treated
as potentially biohazardous.
» Always follow local and national safety regulations
related to storage, handling and disposal of
chemicals, biological samples and waste.
» Read and understand the safety data sheet (SDS)
provided by the chemical manufacturer before
storing, handling, working with, or disposing
of any chemical or hazardous substance.
» Personnel working with or near the system must wear
protective clothing, safety gear, and eye protection that
comply with local and national safety regulations.
Maintenance and Troubleshooting
» If the system is found with the door closed and the
power off, ensure to ventilate the system properly before
turning the system back on. Follow the instructions
in the "Explosion Risk" section on page 9.
» If the fan stops working, shut down the system,
remove any solvents, samples, and waste inside the
system, and contact Biotage 1-Point Support.
» If the ventilation is too low, potentially harmful gases will
escape into the surrounding environment. Ensure to take the
necessary precautions to avoid exposure to harmful gases.
» Follow all maintenance instructions in the “Maintenance”
chapter of the “Biotage®Extrahera™User Manual” document.
» Clean the waste tubing regularly to avoid leakage
caused by the tubing getting clogged. See instructions
in the “Biotage®Extrahera™User Manual” document.
» There are no user serviceable parts inside the system.
Covers and safety shields may only be removed by an
authorized Biotage service engineer. Potential electrical
hazard exists due to high voltage circuits inside the system.
» The power cord should be inspected periodically
and replaced if damaged or altered. Use only
a power cord supplied by Biotage.
» The system uses double pole fusing. Use only
exact replacement fuses supplied by Biotage.
Incorrect fuses create a potential fire hazard.
» Use only tubing, nuts, and ferrules supplied by Biotage.
» Use caution when finger-tightening fittings to
prevent stripped threads or crushed ferrules.


Safety
Restriction of Hazardous
Substances (RoHS) Directive
The RoHS directive is a European Union-derived initiative
in which the elimination of certain hazardous substances
is the key objective. The elimination of these substances
will contribute to the protection of human health and the
environmentally sound recovery and disposal of equipment.
WEEE Compliance Statement
Valid for customers in EU countries
We are committed to being a good corporate
citizen. As part of that commitment, we strive to
maintain an environmentally conscious
manufacturing operation. The European Union (EU)
has enacted a directive on product recycling
(Waste Electrical and Electronic Equipment, WEEE).
Products falling under the scope of the WEEE Directive are
identified with a crossed over “wheelie bin” symbol on the
product label, as indicated to the left. To forward a product for
recycling or proper disposal, return them to Biotage Sweden AB.
Before forwarding a product for recycling or disposal, it should
be emptied of all liquid and cleaned from harmful residues.
When returning a product to Biotage, this should be done
in accordance with the material return procedures supplied
separately by Biotage.
Safety in Other Languages
Translated versions of the safety chapter can be downloaded
at www.biotage.com. If you have problems downloading the
safety translations (“Biotage®Extrahera™Safety Translations”),
please contact your local Biotage representative. See contact
information on the back of this document or visit our website
www.biotage.com.

 Biotage®Extrahera™Installation and Safety | © Biotage 2020
General Information
General Information
Consumables and Accessories
Only genuine Biotage accessories must be used in the system.
To order consumables and accessories, see contact information on
the back of this document or visit our website www.biotage.com.
Manufacturer
Biotage GB Limited United Kingdom for
Biotage Sweden AB
Contact Us
Biotage Sweden AB
Box 8
SE-751 03 Uppsala
SWEDEN
Visiting address: Vimpelgatan 5
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]
Website: www.biotage.com
Please contact your local Biotage representative. See contact
information on the back of this document or visit our website
www.biotage.com.

© Biotage 2018
Title Truncated if Neccessary... | Page 
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