Bioneer Exicycler 96 User manual

Exicycler™ 96 Real-Time Quantitative Thermal Block
Petunjuk Instalasi dan Kalibrasi
Exicycler™ 96 Real-Time Quantitative Thermal Block

Garansi dan Kewajiban
Semua produk Bioneer telah diuji di bawah pengawasan prosedur kontrol kualitas yang ketat. Bioneer menjamin kualitas
instrumen selama periode garansi. Harap laporkan segala masalah yang ditimbulkan oleh instrument secepatnya.
Kewajiban Bioneer merupakan hal yang kondisional sesuai dengan kesediaan pelanggan untuk berbagi rincian masalah
yang terjadi pada Bioneer. Harap segera laporkan masalah yang terjadi dalam waktu kurang dari 30 hari.
Pengujian Kontrol Kualitas
Setiap lot produk Bioneer telah diuji oleh Tim Kontrol Kualitas Bioneer.
Catatan
Menunggu Paten
Exicycler merupakan merek dagang Bioneer Corporation.
Aplikasi tertentu untuk produk ini dilindungi oleh paten yang sudah diajukan atau ditetapkan pada negara-negara
tertentu. Pembelian produk ini tidak meliputi lisensi untuk melakukan aplikasi yang telah dipatenkan, pengguna produk ini
harus memperoleh lisensi paten tergantung dari jenis aplikasi serta negara tempat produk ini digunakan. Untuk informasi
lebih lanjut Manager Legal, Bioneer Corporation, 49-3, Munpyeong-dong, Daedeok-gu, Daejeon 306-220, Korea selatan.
Tampilan dan spesifikasi dapat berubah sewaktu-waktu tanpa pemberitahuan.
Hak Cipta ©Oktober 2011 oleh Bioneer Corporation.
Rev 090815

Daftar Isi
Sebelum Mulai ....................................................................................................................................4
Petunjuk Keselamatan ........................................................................................................................9
Pertimbangan lokasi dan instalasi ............................................................................................9
Pengoperasian dan perawatan .................................................................................................10
Standar EMC dan Keselamatan Eropa (CE) ...............................................................................12
Pengantar............................................................................................................................................15
Konponen dan Spesifikasi Sistem ......................................................................................................19
Komponen Sistem ....................................................................................................................19
Spesifikasi ................................................................................................................................20
Tampilan Sistem ...................................................................................................................... 22
Instalasi ...............................................................................................................................................25
Pengaturan Komputer.............................................................................................................. 25
Menghubungkan Komputer dan Exicycler™ 96 ......................................................................... 26
Menginstal Software Operasi dan Analisis ................................................................................ 27
Pengoperasian Sistem dan Preparasi Kalibrasi ...................................................................................35
Menghidupkan Instrumen dan Diagnosis Diri .......................................................................... 35
Produk Reagen dan Bahan Habis Pakai ......................................................................................36
Kalibrasi Menggunakan ExiCfg..............................................................................................................45
Sebelum Memulai Kalibrasi dengan Kit Kalibrasi ....................................................................... 45
Preparasi Plate Kalibrasi ............................................................................................................. 45
Kalibrasi Menggunakan ExiCfg .................................................................................................. 48
Mask Calibration ....................................................................................................................... 48
Background Calibration...............................................................................................................51
Multi-channel Calibration .......................................................................................................... 55
Memulai Software Run Exicycler................................................................................................. 63
Melakukan Diagnosis Sistem dengan ExiCfg .............................................................................. 79
Pemecahan Masalah .............................................................................................................................85
Informasi Pemesanan dan Garansi........................................................................................................91

Terbiasa dengan pengoperasian sistem Microsoft Windows XP.
Sebelum Memulai
Bagaimana Cara Menggunakan Buku Petunjuk Ini
Buku ini ditujukan untuk
Buku Petunjuk ini ditulis untuk peneliti dan staf laboratorium yang bertanggung jawab untuk menginstal
dan merawat Exicycler™ 96 Real-Time Quantitative Thermal Block.
Kebutuhan Umum untuk Instalasi
Buku petunjuk ini mengasumsikan bahwa Anda:
•
•
•
® ®
Memiliki teknik dasar untuk menangani sampel DNA dan RNA untuk PCR
Memiliki teknik dasar untuk menyimpan maupun mengkopi data pada komputer.
Konvensi Kata



Penulisan Tebal (Bold) menandakan aksi pengguna seperti mengetik teks. Contoh: Ketik untuk
Test dan klik OK melanjutkan tahap selanjutnya
Penulisan Miring (Italic) represents important words or sentences and is also used for emphasis. For
example:
After analysis, you must save data using Save As.
A right arrow in bold (>) separates consecutive commands you select from a main menu or
shortcut menu. For example:
File> Config> Scan
Petunjuk Keselamatan dan Peringatan
The following safety alert words are used in this manual and require a particular level of observation.
Each alert word is defined as below:
Note: Indicates relevant or helpful information about an instrument, however doesn‟t affect instrument
operation.
Important: Indicates information that is necessary for proper instrument operation.
Danger: Indicates an imminently hazardous situation which, if not avoided, could result in serious injury.
This safety word is used to alert improper instrument operation.
Warning: Indicates a potentially hazardous situation which, if not avoided, will result in death or serious
injury if not avoided.

Exicycler™ 96 Real-Time Quantitative Thermal Block
Petunjuk Keselamatan

Exicycler ™ 96 Real-Time Quantitative Thermal Block
Safety Warnings and Cautions
Safety instructions in this guide book are to cover possible dangerous conditions and situations that
may occur. It is your responsibility to read this guide thoroughly when installing, maintaining, or
operating Exicycler™ 96. The following safety alert words will be used in this section:
Warning: Hazards or unsafe actions that may result in severe injury.
Caution: Hazards or unsafe actions that may result in minor injury or damage.
Warnings: Hazards or unsafe actions that may result in High temperature and Skin burn.
Warnings: Hazards or unsafe actions that may result in Electronic risk.
Location Consideration and Installation
General Instrument Safety
1) Check the power voltage rating before connecting the instrument to an electrical outlet.
Exicycler™ 96 system is configured for either 110V or 220V (±10%). Using AVR (Automatic
Voltage Regulator) or UPS (Uninterrupted Power Supply) is recommended for uninterruptible
power supply.
2) The Exicycler™ 96 must be grounded for protection against electric shock. If not, it may cause
serious injury and system damage.
3) Do not use a loose power cable or connector. An overheated power cable may break and lead
to a fire or electric shock.
4) If you use a power extension cable, do not connect too many devices to it and operate them at
the same time. Overload may occur and cause a fire.
5) Dry your hands completely when handling a power cord for protection against electric shock.
6) Do not place any objects in front of the main door of the Exicycler™ 96 that can interrupt door
operation.
7) Leave 30 cm space between the Exicycler™ 96 and the wall for proper ventilation.
8) Do not install the Exicycler™ 96 in a dusty environment to help prevent false operation or
technical damage.
9) Keep the Exicycler™ 96 away from heat sources.
10)The Exicycler™ 96 must not be installed in an area where it is exposed to water or is humid. It
may cause electric shock, a fire, or system damage.
11)The Exicycler™ 96 must not be installed in an area where it is exposed to combustible or
flammable vapor. In the case of a gas leak, open the windows and let fresh air in. Do not
operate any electrical switch during a gas leak. It may cause explosion or fire.
12)Do not disassemble or repair the Exicycler™ 96 yourself. It may cause a fire, electric shock,
9

Exicycler ™ 96 Real-Time Quantitative Thermal Block
and system damage. A limited warranty does not cover unauthorized alterations or damage by
abuse.
Installation Safety
1) Place and install the Exicycler™96 away from direct sunlight.
2) It is recommended that you turn off the computer as well as unplug the power cable before
connecting it to the Exicycler™ 96. When the computer power is on while connecting, the
communication port connector in either the computer or the Exicycler™ 96 may get damaged.
3) Make sure that the USB cable is firmly connected between the Exicycler™ 96 and the
computer. Unstable connection may cause damage of the communication port connector or
data transfer errors.
4) The built-in camera in the Exicycler™ 96 is a static-sensitive device. Pay particular attention to
any cables connected to the Exicycler™ 96 to avoid static damage.
Cautions
1) Ensure the power cable is clean and connect it firmly to the Exicycler™ 96.
2) Operate the Exicycler™96 in a place where the temperature is always between 15℃and 30℃.
Poor performance is influenced by extreme temperatures. High temperatures can cause mis-
operation and poor performance.
3) Operate the system in a place where the humidity is always between 20 % and 80% with no
condensation. High humidity conditions can cause corrosion of internal components and low
humidity can lead to errors.
4) Do not place any objects behind or by the side of the Exicycler™96 that can interrupt
ventilization and cause errors.
5) The internal optical components of the Exicycler™96 may get damaged if the instrument falls
or is exposes to excessive physical shock.
6) Unplug the power cable from the Exicycler™96 when not in operation for a long period of time
to prevent the possibility of fire by overheating.
Operation and Maintenance
Warnings
1) The system can be hazardous if misused.
2) Keep the 96-well thermal block area clean to prevent damage and to generate accurate
experimental data.
3) After any Real-Time PCR runs including „Scan‟or „Melting‟, allow the Exicycler™ 96 at least 10
minutes to cool down the light source lamp. Continuous operation without a break will reduce
the lamp life time and cause errors.
10

Exicycler ™ 96 Real-Time Quantitative Thermal Block
4) Do not place a piece of paper or a plastic cover under the Exicycler™96. It could cause fire.
5) Do not turn off the Exicycler™96 right after a Real-Time PCR run is done. Wait until the
cooling fan stops running completely. The cooling fan still runs for about 2 minutes to cool
down the lamp after every Real-Time PCR run.
6) Do not cover the Exicycler™96 with a piece of paper or a plastic cover. It may cause a fire or
failure.
7) Set ‟Turn off monitor‟, „Turn off hard disks‟, „System standby„, and „System hibernates‟to
„Never‟using „Power Options Properties‟in the Control Panel. Otherwise, data transfer
between the Exicycler™96 and the computer will be interrupted.
IMPORTANT:
Exicycler™ 96 should be operated in clean condition. Contaminants such as dust can cause troubles and reduce the life
time of Exicycler™ 96. Please prevent dust from coming into Exicycler™ 96. Through removing dust periodically from the
Exicycler™ 96 system, the life time can be extended.
11

Exicycler ™ 96 Real-Time Quantitative Thermal Block
European Safety and EMC Standards (CE)
This instrument meets the requirements of the European Directive for in vitro diagnostic medical
devices 98/79/EC. This instrument has been tested according to the standards listed below and
complies with them.
1) Council Directive 98/79/EC of 27 October 1998 concerning in-vitro diagnostic medical devices
2) EN 61010-1 [2001] Safety requirements for electrical equipment for measurement, control and
laboratory use. Part 1: General requirements for safety.
3) EN 61010-2-010 [2003] Safety requirements for electrical equipment for measurement, control
and laboratory use. Part 2-010: Particular requirements for laboratory equipment for the
heating of materials.
4) EN 61010-2-081 [2001] Safety requirements for electrical equipment for measurement, control
and laboratory use. Part 2-081: Particular requirements for automatic and semi-automatic
laboratory equipment for analysis and other purposes.
5) EN 61010-2-101 [2002] Safety requirements for electrical equipment for measurement, control
and laboratory use. Part 2-101: Particular requirements for in vitro diagnostic (IVD) medical
equipment.
6) EN 61326 [1997+A1] [1998+A2] [2001+A3] [2003 Class A] Electrical equipment for
measurement, control and laboratory use - EMC requirements.
7) EN 60601-1-2 [2001] Medical electrical equipment. Part 1-2: General requirements for basic
safety and essential performance - Collateral standard: Electromagnetic compatibility -
Requirements and tests.
Compliance is demonstrated by the following mark:
Authorized Representative
MT Promedt Consulting GmbH
Altenhofstrasse 80
D-66386 St. Ingbert
Germany
Product Use Limitations
The Exicycler™ system is designed and sold for in vitro diagnostic (IVD) applications in combination
with the diagnostic kits manufactured by Bioneer and labeled for diagnostic purposes. The Exicycler
TM
system can also be used for life science research and other laboratory purposes.
12

Exicycler™ 96 Real-Time Quantitative Thermal Block
Pengantar

Exicycler™ 96 Real Time Quantitative Thermal Block
Introduction
The Exicycler™ 96 Real-Time Quantitative Thermal Block combines a thermal block in a standard 96-
well format and an innovative fluorescence detector. The detector monitors the fluorescence emitted as
an indicator of amplified nucleic acid product during each PCR cycle in real time.
The thermal block is built in the lower part of the Exicycler™ 96, which carries out a thermal cycling. The
detector is located in the upper part of the Exicycler™ 96, which measures the fluorescence emitted
from samples in the thermal block in real time and transfers data to the computer for analysis. Data
transferred from the detector is analyzed with the Exicycler ™ Analysis Software.
The Exicycler™ 96 Real-Time Quantitative Thermal Block provides a maximum ramping rate of
2.5℃/sec, and features various funtions such as gradient, time increment, temperature increment, and
ramp rate control for myriad applications.
The Exicycler™ 96 Real-Time Quantitative Thermal Block detector consists of an light source part
and a detection part. The light source part is an energy source to excite fluorescent dyes. A short arc
lamp that has a long lifetime is bright, and is implemented. A white-light source is divided into particular
wavelength groups by band path filters. The band path filters that are set in the Exicycler™ 96 covers
within 480 to 680 ㎚and works as a set consisting of an excitation and an emission filter. Five of the
filter sets are provided for various applications. Therefore, adding additional band path filters is not
required.
The detector, a hight sensitive 2D CCD camera, provides high sensitivity and detects fluorescence
signals from a 96-well plate in the thermal block all at one time. This simultaneous detection has a great
advantage over sequential detection. The innovative detector invented by Bioneer has also reduced
well-to-well variation and has minimized dye-to-dye interference, therefore providing more accurate
results. Bioneer‟s state-of-the-art technologies applied to the Exicycler™ 96 are to generate a uniform
light surface over the thermal block, to detect multiple fluorescent signals emitted from various dyes
through the light surface, and to separate the signals within a selective wavelength range of each dye.
The Exicycler™ 96 Real-Time Quantitative Thermal Block features an auto loading function for
automation reducing errors and Self-diagnosis for diagnosing systematical conditions of the Exicycer™
96 for users convenience. The Exicycler™ 96 Software is composed of three main programs.
1)
2)
3)
A set up program for calibration, diagnosis and confirmation of the instrument‟s information.
An operation program for creating a protocol, assigning a probe and plate, saving & displaying
data while operating the Exicycer™ 96.
An analysis program for data analysis. The analysis program includes Absolute Quantification,
Relative Quantification, Multi-Relative Quantification, SNP genotyping,
Existentence/Nonexistentence. Analysis and is applicable to Gene expression, Quantification of
cell and virus, and SNP genotyping.
15

Exicycler™ 96 Real-Time Quantitative Thermal Block
Komponen dan Spesifikasi Sistem

Exicycler™ 96 Real-Time Quantitative Thermal Block
Komponen dan Spesifikasi Sistem
Exicycler™ 96 Quantitative Thermal Block telah dijamin dan digaransi kualitas material dan pembuatan
oleh Bioneer selama satu tahun. Bioneer akan mengenakan biaya perbaikan untuk produk dengan
kondisi sebagai berikut :
Kerusakan karena penggunaan dari pelangan.
Produk diperbaiki setelah periode garansi.
Sebelum menginstal Exicycler™ 96, pastikan kelengkapan semua komponen sistem sebagai berikut :
Komponen Sistem
No. Katalog. A-2060 Cek
Instrumen Utama
Kabel USB 2.0 high speed
Kabel Power
Software
Buku Manual
96 well reaction plate
Optical sealing tape
Optical tape sealing applicator
Surat Garansi
Formulir Permintaan Servis
1
1
1
1 CD
1
1 pak
1 pak
1
1
1
□
□
□
□
□
□
□
□
□
□
19

Exicycler™ 96 Real-Time Quantitative Thermal Block
Spesifikasi
Spesifikasi Fisik
Dimensi (mm)
Berat (kg)
Ukuran kapasitas sampel
Volum Sampel
Voltase
Frekuensi
Tenaga
Spesifikasi Pengoperasian
Metode Pemanasan / Pendinginan
Kisaran Suhu
Akurasi Suhu
Keseragaman Suhu
Kecepatan Ramping
Suhu Lid
Kisaran Gradient
Kisaran Kenaikan Suhu
Kisaran Kenaikan Waktu
Kontrol kecepatan Ramp
Suhu Pengoperasian
Kelembapan
Komunikasi
Sistem Operasi OS
Komponen Optik
Sumber Cahaya
Watt
355(P) X 540(L) X 470(T)
30 kg
96-well plate / 0.2 ㎖tabung mikro
20~50 ㎕
(50 ㎕direkomendasikan)
100 ~ 240 VAC
50 / 60 ㎐
850 Watts (Fuse : 250 V, F10AL)
Peltier
4.0℃~ 99.9℃
±0.3℃
±0.5℃
Maks. 2.5℃/detik
antara 90 ~ 120℃
1℃~20℃
0.1℃~9.9℃
1 detik ~ 60 detik
0.1℃/detik
15 ~ 30℃
20 ~ 80%
USB 2.0 high speed
Windows XP
Lampu Short arc
120 W
*
Waktu Hidup Lampu
3,000 jam
Sensor 16 bit 2D CCD
**
Filter emisi / eksitasi
5 Tipe / 5 Tipe
* Menghidupkan / mematikan lampu terus-menerus dapat mengurangi waktu hidup lampu.
* Lampu akan menghasilkan panas ketika beroperasi. Pastikan untuk mematikan Exicycler™ setelah
kipas pendingin selesai beroperasi (kipas pendingin akan berhenti setelah kira-kira 2 menit). Perhatikan
tabel berikut untuk keterangan lebih lanjut mengenai set filter.
20

Exicycler™ 96 Real-Time Quantitative Thermal Block
† Set Filter
Posisi
1
2
3
4
5
6
Eksitasi (nm)
Kosong
490
520
550
580
640
Emisi (nm)
Kosong
520
550
580
610
670
Set
1
1
1
1
1
1
Pewarna Fluoresens
-
FAM, SYBR Green I
JOE, TET
TAMRA, CY3
Texas Red, ROX, Red610
CY5, Red670
Peringatan :
Bahaya Kimia: Pewarna fluoresens mungkin dapat menyebabkan iritasi pada mata, kulit atau saluran pernapasan. Baca
dokumen MSDS sebelum digunakan dan ikuti petunjuk pada dokumen untuk menangani iritasi jika substansi tertelan
atau terhirup. Selalu gunakan pakaian pelindung, sarung tangan dan pelindung mata sebagai pencegahan.
21

Exicycler™ 96 Real-Time Quantitative Thermal Block
Tampilan Sistem
Tampilan Depan
PINTU
Tampilan Belakang
POWER and Status LED
Kabel Komunikasi USB
Tombol On/Off
Lubang Ventilasi
Tampilan Dalam
Blok Pemanas 96-well
22

Exicycler™ 96 Real-Time Quantitative Thermal Block
Instalasi

Exicycler™ 96 Real-Time Quantitative Thermal Block
Instalasi
Untuk mengoperasikan Exicycler™ 96 Real-Time Quantitative Thermal Block, driver kamera dan
software pengoperasian harus diinstal pada komputer terlebih dahulu. Untuk penginstalan driver dan
software, ikuti petunjuk pada bagian berikut.
Pengaturan Komputer
Exicycler™ 96 Real-Time Quantitative Thermal Block dipoerasikan melalui komputer, oleh karena itu
computer atau laptop sangat dibutuhkan untuk menginstal Exicycler™ 96. Komputer harus memenuhi
syarat sesuai dengan daftar dibawah. Harap hubungi pusat servis Bioneer jika ada pertanyaa seputar
instalasi.
Spesifikasi Komputer
•Intel Dual Core E2160 (1.8GHz) atau versi terbaru
•Sistem Operasi Window XP dalam Bahasa Korea atau Inggris (Service pack 2 atau versi terbaru)
•1.0GB RAM atau lebih tinggi
•Resolusi layar 1280 * 1024
•Port USB 2.0 high speed
•Minimum 20GB Hard disk drive
•Microsoft Excel (Pilihan)
Untuk pertanyaan seputar masalah dengan komputer atau sistem operasi harap hubungi produsen atau
pembuat komputer.
1) Komputer harus memiliki paling tidak 1 USB port untuk komunikasi data.
2) Direkomendasikan untuk menginstal software antivirus untuk membantu mengoperasikan komputer
dengan aman. Firewall sebaiknya disiapkan untuk mencegah kebocoran data pada jaringan internal.
Harap hubungi bagian IT Anda untuk menyiapkan antivirus dan firewall.
3) Direkomendasikan untuk tidak menginstal stik memori pada saat Exicycler™ 96 beroperasi karena dapat
menyebabkan kesalahan teknis antara Exicycler™ 96 dan komputer.
25

Exicycler™ 96 Real-Time Quantitative Thermal Block
Menghubungkan Komputer dan Exicycler™ 96
Terdapat port penghubung utama (power) dan port komunikasi USB pada bagian belakang Exicycler™
96. Komputer harus dalam keadaan mati ketika dihubungkan dengan Exicycler™ 96 untuk mencegah
kerusakan pada port komunikasi. Perhatikan instruksi berikut ini untuk menghubungkan komputer
dengan Exicycler™ 96
1) Keluarkan Exicycler™ 96 dari kemasan dan pastikan semua komponen sudah lengkap. Lihat daftar
komponen dibawah.
2) Exicycler™ 96 harus diinstal pada area yang tidak terkena sinar matahari langsung dan harus
ditempatkan pada permukaan yang stabil dan datar.
3) Siapkan komputer pada tempat instalasi.
4) Tempatkan Exicycler™ 96 pada tempat yang dituju dengan hati-hati.
5) Hubungkan kabel USB 2.0 dengan port koneksi USB pada bagian belakang Exicycler™ 96,
kemudian hubungkan kabel dengan komputer.
6) Connect the power cable to the Exicycler™ 96, then to the receptable wall circuit.
List of Components
No Katalog. A-2060 Cek
Instrumen utama
Kabel USB 2.0 high speed
Kabel Power*
Software
Buku Petunjuk Operasi
96 well reaction plate
Optical sealing tape
Optical tape sealing applicator
Dokumen Garansi
Formulir Permintaan Servis
1
1
1
1 CD
1
1 pack
1 pack
1
1
1
□
□
□
□
□
□
□
□
□
□
* Disediakan sesuai dengan standard negara .
26

Exicycler™ 96 Real-Time Quantitative Thermal Block
Menginstal Software untuk Operasi dan Analisis
Untuk mengoperasikan Exicycler™ 96 Real-Time Quantitative Thermal Block, software operasi dan
driver kamera harus diinstal terlebih dahulu. Saat pertama pengoperasian, harap perhatikan instruksi
berikut agar dapat menginstal dengan baik. Jika hanya ingin menginstal software untuk keperluan
analisis data, ikuti petunjuk pada bagian selanjutnya.
PENTING:
Jangan menyalakan Exicycler™96 sebelum Anda menginstal software untuk operasi serta analisis dan driver kamera.
Instal software untuk operasional terlebih dahulu sebelum driver kamera.
1) Hanya hidupkan komputer, jangan hidupkan Exicycler™ 96.
Masukkan CD untuk instalasi ke dalam kumputer. Set up
program Exicycler™ 96 akan mulai secara otomatis. Jika
program tidak mulai secata otomatis, buka My Computer
untuk membuka isi CD dan klik Setup.exe.
2) Akan muncul Exicycler™ 96 Install Shield Wizard. Klik
Next untuk memulai proses install. Instalasi akan
berlanjut pada C:\Exicycler3.
3) Klik Next untuk memulai install software.
27