Tuttnauer LABSCI 11LV ELVC User manual

Operation and Maintenance
Manual
Electronic Laboratory Autoclaves
Models LABSCI 11LV
This device is not a medical device and not intended for medical use.
LV-D: standard autoclave
C: optional system for fast cooling
PV: optional vacuum pump
BH: optional bio hazard filtration
WR: water recycling system
F: optional fan for super-fast cooling
Cat. No. MAN205-0468003EN Rev G
Heidolph North America , 1235 N Mittel Blvd. Suite B Wood Dale IL 60191
Phone: [phone] Fax: [phone], e-mail: [e-mail]

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Table of Contents
Paragraph page no.
1. GENERAL INFORMATION
1.2 Available optional configurations ...............................................................5
1.3 Incoming Inspection....................................................................................6
1.4 Warranty......................................................................................................6
1.5 Warranty Statement.....................................................................................7
2. Safety Instructions................................................................................................8
3. Technical Data....................................................................................................10
3.1 Operating Conditions.................................................................................10
3.2 Directives and Standards...........................................................................11
3.3 Ordering Information................................................................................11
3.4 Accessories.................................................................................................11
3.5 Environment Emission Information .........................................................12
3.6 Electrical Data...........................................................................................12
3.7 Water quality..............................................................................................12
3.8 Specification ..............................................................................................14
3.9 Construction ..............................................................................................15
3.10 Utilities.......................................................................................................15
3.11 Symbol Description....................................................................................16
3.12 Front View.................................................................................................17
3.13 Rear View...................................................................................................20
3.14 Rear View: What is Special for WR Configuration ..................................22
3.15 Overall Dimensions, WR Configuration(mm)...........................................23
3.16 Safety Features..........................................................................................23
3.17 Description of Operation ...........................................................................24
4. Control Panel......................................................................................................26
4.1 Control Panel Drawing..............................................................................26
4.2 Description and Functions of the Front Panel Keyboard.........................27
4.3 Displayed Error Messages / Symbols.........................................................29
4.4 Displayed operational messages / Symbols................................................29
5. Sterilization Programs........................................................................................31
5.1 Program 1: Glass.......................................................................................35
5.2 Program 2: Plastic.....................................................................................36
5.3 Program 3: Liquid A..................................................................................37
5.4 Program 4: Liquid B –Waste....................................................................38
5.5 Program 5: Liquid A –Cooling (C –cooling models only).......................39
5.6 Program 6: Liquid B –Waste Cooling (C –cooling models only)............40

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5.7 Program 7: Bio Hazard 1 (BH, bio-hazard models only) .........................41
5.8 Program 8: Bio Hazard 2 (BH, bio-hazard models only) .........................42
5.9 Program 9: Bio Hazard Liquids (BH, bio-hazard models only) ...............43
5.10 Program 10: Vacuum Test (PV, pre-vacuum models only) ......................44
5.11 Program 11: Warm-Up..............................................................................46
5.12 Program 12: Isothermal ............................................................................47
5.13 Program 13: Air Steam Mixture................................................................48
5.14 Program 14: Glass Test .............................................................................49
5.15 Program 15: Durham ................................................................................50
6. Checking and Changing Parameters and Other Data .......................................52
6.1 Browsing through the menus ....................................................................52
6.2Changing a Parameter...............................................................................53
6.3 Quick options screen..................................................................................55
6.4 Logging in and entering the Main menu ..................................................59
6.5 Changing parameters ................................................................................61
6.6 System Parameters.....................................................................................62
6.7 Maintenance ..............................................................................................62
6.8 Cycle parameters........................................................................................64
7. Screens................................................................................................................66
7.1 Screens following a complete successfully cycle –"Cycle Ended" ..........66
7.2 Screens following aborted cycles after complete sterilization stage..........67
7.3 Screens following a fail cycle:...................................................................67
8. Printer.................................................................................................................69
8.1 Printer Output............................................................................................69
8.2 Printer Handling........................................................................................72
9. Preparation before Sterilization .........................................................................76
9.1 Instruments................................................................................................76
9.2 Liquids .......................................................................................................77
9.3 Loading......................................................................................................77
10. Operating Instructions........................................................................................79
10.1 Draining and filling the water reservoir (WR only)..................................79
10.2 Turning on the autoclave ..........................................................................80
10.3 Opening the door.......................................................................................81
10.4 Loading......................................................................................................81
10.5 Closing the door.........................................................................................81
10.6 Starting cycle .............................................................................................81
10.7 Unloading ..................................................................................................82
10.8 Stopping the process and cancelling the ERROR message.......................82
10.9 Start Cycle by Clock mode.........................................................................84
10.10 Moving the Autoclave................................................................................84

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10.11 Loading and Unloading the Device...........................................................84
11. Service and Maintenance ...................................................................................86
11.1 Preventive Maintenance ............................................................................86
11.2 Checking the Safety Valve.........................................................................87
12. Troubleshooting..................................................................................................90
13. Baskets and Containers ......................................................................................95
14. Spare Parts List...................................................................................................95
15. Accessories..........................................................................................................96

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1. General Information
1.1 Introduction
Attention!
This device is not a medical device and not intended for
medical use.
LABSCI 11LV is a vertical sterilizer designed especially for sterilization
of instruments, liquids and other materials in hospital laboratories,
laboratories & research institutes, food laboratories and pharmaceutical
facilities.
The sterilizer is fully automatic with a choice of ten programs (including
two custom programs), eliminating any need for operator intervention
during a cycle. The custom programs are programs that may be
changed by an authorized technician. A computerized control unit
enables precise control and monitoring of physical parameters and
clear documentation of the sterilization cycles.
The autoclave is equipped with a safety valve, which blows off at 280
kPa (40 psi) (max. working pressure). The safety valve is located on
the rear of the autoclave. The control system provides adequate
protection, to ensure the safety of personnel and reliable operation with
a minimum of down time.
Printer is an optional addition to the autoclave. The printer prints the
preset and actual parameters of the cycle (temperature, time, and
pressure/vacuum).
The autoclave is equipped with an analog pressure gauge designed for
reference only. In case that there is any problem with electricity supply
during a sterilization cycle the pressure gauge will be used to verify that
there is pressure in the chamber.
The temperature and pressure are controlled through sensors placed
inside and outside the media container or bottles.
This manual is intended to give the user a general understanding of
how the autoclave works and indicates best ways to operate and
maintain it to obtain optimum results and a trouble-free operation.

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After reading this manual, operating the autoclave should be straight
forward. However, since the autoclave is built using high technology
sensitive components, no attempt should be made by the user or any
other unauthorized person to repair or re-calibrate it.
Only technical personnel having proper qualifications, holding
technical documentation and adequate test instrumentation, are
authorized to undertake repair or service.
1.1 Available optional configurations
LV Is the basic model of the vertical laboratory autoclave.
C
A special feature of the C models is the fast cooling stage for liquids. In
this stage pressure in the chamber is increased by means of
compressed air to compensate the fast decreasing of pressure due to
the fast cooling. The fast cooling shortens the time required for safe
handling of bottles.
Note: There is no cooling option for WR configuration.
PV
Special feature is the vacuum pump that enables air removal prior to
sterilization and during the drying stage.
The advantages of the pre-vacuum sterilizer in comparison to the
regular gravity displacement steam sterilizer are as follows:
•Removal of air pockets from packs and porous loads and most kinds
of tubes (rubber, plastic etc.) by vacuum at the first stage of the cycle.
•Better steam penetration into the load; resulting in effective
sterilization.
•Better temperature uniformity.
•Better drying of materials with closed doors due to the vacuum
achieved in the chamber at the end of the sterilization cycle.
A special feature of this autoclave is the fast cooling stage for liquids.
In this stage pressure in the chamber is increased by means of
compressed air to compensate the fast decreasing of pressure due to
the fast cooling. The fast cooling shortens the time required for safe
handling of bottles
BH
In BH (bio-hazard) models, a bio-hazard filter installed in the chamber
enables sterilizing bio-hazard load without contaminating the
surrounding.

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WR
In WR (Water Recycling) models, water is saved by the water saving
system, because it recycles the water and re-uses it for each cycle in
the following way:
•Water inlet by the pump from the reservoir and not direct from the
plumbing.
•On slow/fast exhaust, the water gets into the reservoir.
F There is a configuration equipped with fan that allows to shorten the
cooling operation.
1.2 Incoming Inspection
The autoclave should be unpacked and inspected for mechanical
damage upon receipt. Observe packing method and retain packing
materials until the unit has been inspected. Mechanical inspection
involves checking for signs of physical damage such as: scratched
panel surfaces, broken knobs, etc.
If damage is apparent, contact your dealer or point of purchase, so that
they may notify the manufacturer and file a claim with the appropriate
carrier.
All Tuttnauer products are carefully inspected prior to shipment and all
reasonable precautions are taken in preparing them for shipment to
assure safe arrival at their destination.
1.3 Warranty
We certify that this instrument is guaranteed to be free from defects in
material and workmanship for one year against faulty components and
assembly except for glassware, lamps, and heaters.
The warranty does not include and does not replace routine
treatment and preventive maintenance to be performed according
to instructions in sec. 11.1 (Preventive Maintenance).
Our obligation is limited to replacing the instrument or parts, after our
examination, if within one year after the date of shipment they prove to
be defective. This warranty does not apply to any instrument that has
been subjected to misuse, neglect, accident or improper installation or
application, nor shall it extend to products that have been repaired or
altered outside the factory without prior authorization from us.
The Autoclave should not be used in a manner not described in
this manual!

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1.4 Warranty Statement
The warranty registration must be completed and returned to our
service departments; within fourteen (14) days of purchase or the
warranty will be void.
Our Technical Service Depts can be reached at:
Heidolph North America, 1235 N Mittel Blvd. Suite B Wood Dale
IL 60191
Service & Repair Division
Troubleshooting, Service incidents, Warranty repair, Repair, Services
and Return authorization
: 224-265-9600 Fax: [phone] e-mail: [email protected]
Tuttnauer USA Co., Ltd., 25 Power Drive Hauppauge, NY 11788,
USA : [phone], [phone], Fax: [phone]
e-mail:[email protected].
Note:
If there is any difficulty with this instrument, and the solution is not
covered in this manual, contact our representative or us first. Do not
attempt to service this instrument yourself. Describe the difficulty as
clearly as possible so we may be able to diagnose the problem and
provide a prompt solution.
If the autoclave is equipped with a printer, send along a copy of the last
printout for our inspection. If replacement parts are needed, stipulate
the model and serial number of the machine.
No products will be accepted for repair without proper authorization
from us. All transportation charges must be paid both ways by the
owner. This warranty will be void if the unit is not purchased from an
authorized full service Tuttnauer dealer.

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2. Safety Instructions
The autoclave has unique characteristics. Please read and understand
the operation instructions before first operation of the autoclave. The
following issues may require instructions guidance provided by the
manufacturer: how to operate the autoclave, the door safety
mechanism, the dangers involved in circumventing safety means, how
to ensure that the door is closed, and how to select a correct
sterilization program.
Make sure that you know where the main power switch is, where the
water cut-off valve is and where the compressed air disconnection
valves (if applicable) are located.
Autoclave maintenance is crucial for the correct and efficient function of
the device. We enclose a log booklet that includes maintenance
recommendations, with every device.
The weekly spore test is part of the preventive maintenance plan, along
with the annual validation of the sterilization processes that ensures
appropriate temperature dispersion within the chamber.
Never use the autoclave to sterilize corrosive products, such as: acids,
bases and phenols, volatile compounds, or solutions such ethanol,
methanol, or chloroform nor radioactive substances.
1. Never start using a new autoclave before the safety, licensing and
authorization department has approved it for use.
2. All autoclave users must receive training in proper usage from an
experienced employee. Every new employee must undergo a training
period under an experienced employee.
3. A written procedure must be established for autoclave operation,
including: daily safety tests, seal inspection and door hinge
inspection, smooth action of the closing mechanism, chamber
cleaning, prevention of clogging and preservation from corrosion,
what is permitted and what is prohibited for sterilization and choosing
a sterilization program.
4. Liquids may be sterilized only with the “liquids” programs. The
container must be covered but not sealed. Sealed bottles may only be
sterilized using a special program. The bottle must be either Pyrex or
a Borosilicate glass bottle. Verify that the two temperature sensors are
located inside two different bottles to assure that the liquid temperature
is 20°C below boiling temperature for sealed bottles at the end of the
cycle.
5. When sterilizing plastic materials, make sure that the item can
withstand sterilization temperature. Plastic that melts in the chamber
is liable to cause a great deal of damage.

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6. Individual glass bottles may be placed within an appropriate container
that will be placed in a basket. Never place glass bottles on the floor
of the autoclave. Never fill more than 2/3 of the bottle volume.
7. On closing the autoclave's door, make sure it is properly locked
before activating.
8. Before withdrawing baskets, wear heat resistant gloves.
9. Before opening the door, verify that there is no pressure in the
chamber (chamber pressure gauge is located on the autoclave's front
panel or door, depends on model).
10. Open the door slowly to allow steam to escape and wait 5 minutes
before you remove the load. When sterilizing liquids, wait 10 minutes.
11. Once a month, ensure that the safety valves are functioning, and
once annually a certified tester must conduct pressure chamber
safety tests.
12. Once annually, or more frequently, effective tests must be performed,
i.e., calibration and validation.
13. Examine the condition of assemblies on a regular basis. Make sure
there are no leaks, breaks, blockages, whistles, or strange noises.
14. It is required to conduct maintenance operations as instructed.
15. Immediately notify the person in charge of any deviation or risk for the
proper function of the device.
16. For WR models, drain the water from the mineral free water reservoir
once a week, and refill with fresh mineral-free water or distilled water
(see 10.1).

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3. Technical Data
3.1 Operating Conditions
The autoclave is intended to work in ‘indoor’ conditions only.
Only autoclavable materials shall be used.
The environment shall not exceed an ambient temperature range of
5ºC (41ºF)-40ºC (104ºF) and a relative humidity of 85% respectively.
The operation altitude shall not be over 2000 meters (6561 feet)
(ambient pressure shall not be lower than 80 kPa (11.6 psi)).
The autoclave shall not be used in a manner not specified in this
manual!
Do not use the autoclave in the presence of dangerous gases.
The packed or unpacked device shall be stored in ‘indoor’ conditions.
Operate the autoclave only in the manner specified in the manual. If
the equipment is used in a manner not specified by the manufacturer,
the protection provided by the equipment may be impaired.
Caution!
Wastewater should be brought into the public net in accordance
with the local rules or requirements i.e. only non-hazardous
liquids shall be disposed in public sewage!

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3.2 Directives and Standards
Every autoclave meets the provisions of the following Directives and is in
compliance with the following Standards:
ISO 9001:
Quality Management System
ISO 14001:
Environmental Management System
ISO 17025:
General requirements for the competence of testing
and calibration autoclaves
ASME Code
Section I and section VIII. Div. I
PED
2014/68/EU
Chinese Regulations
Special Equipment Licensing Office
IEC 61010-1 / UL 61010-1:
Safety requirements for electrical equipment for
measurement, control, and laboratory use –Part 1:
General requirements
IEC 61010-2-040:
Safety requirements for electrical equipment for
measurement, control, and laboratory use –Part 2-040:
Requirements for sterilizers and washer-disinfectors
used to treat medical materials
EN 61326-1:
EMC Requirements for Electrical Equipment
3.3 Ordering Information
Several items must be specified when ordering the unit from your dealer.
•The chamber diameter and chamber depth required
•Please specify the supply voltage available (i.e. 115v/208v; 1Ph/3Ph)
•The temperature scale needed (Celsius or Fahrenheit).
•The pressure scale needed (kPa or psi)
3.4 Accessories
•Baskets –Different size baskets are available for these units. The
baskets are made of stainless-steel wire and have a handle. The
basket allows the operator to load a large quantity of materials into the
chamber.

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The baskets are made of stainless-steel wire and have a handle. The
basket allows the operator to load a large quantity of materials into the
chamber.
•Stainless steel containers - Different size containers are available for
these units.
The containers are designed for sterilizing waste material. The
containers have vent holes along the upper rim.
3.5 Environment Emission Information
A. Peak sound level generated by the sterilizer is « 70 / dBA with a back-
sound level of 60 dBA.
B. Total heat transmitted by the sterilizer is < 240 W/h
3.6 Electrical Data
3.7 Water quality
The distilled or mineral-free water supply shall be according to the table
below:
Suggested maximum limits of contaminants in water for steam
sterilization per EN13060
Substance
Feed Water
Condensate
Evaporate residue
≤ 10 mg/l
≤ 1.0 mg/l
Silicate (SiO2)
≤ 1 mg/l
≤ 0.1 mg/l
Iron
≤ 0.2mg/l
≤ 0.1mg/l
Cadmium
≤ 0.005 mg/l
≤ 0.005 mg/l
Lead
≤ 0.05 mg/l
≤ 0.05 mg/l
Property
Value
Total Power
2400W (2*1200W)
Voltage
230VAC/1Ph
Amperage
10A
Protection against electrical shock
Class I (IEC 61010-1)
Mains supply fluctuation
+/- 10%
Degree of protection by enclosure
IP31

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Rest of heavy metals
except iron,
cadmium, lead
≤ 0.1 mg/l
≤ 0.1 mg/l
Chloride (Cl)
≤ 2 mg/l
≤ 0.1 mg/l
Phosphate
≤ 0.5 mg/l
≤ 0.1 mg/l
Conductivity (at
20°C)
15 µs/cm
≤ 3 µs/cm
pH value
5 to 7.5
5 to 7
Hardness
≤ 0.02 mmol/l
≤ 0.02 mmol/l
Appearance
Colorless, clean, without sediments
Note: The condensate is produced from steam taken from the empty
sterilizer chamber.
Compliance with the above data should be tested in accordance with
acknowledged analytical methods, by an authorized laboratory.
Attention:
The use of water for autoclaves that do not comply with the table
above may have severe impact on the working life of the sterilizer
and can invalidate the manufacturer’s guarantee.
Use only deionized water, having a maximum conductivity of 15
µs/cm. Conductivity greater than 15 µs/cm may cause failures.
Tap water supply
The range of hardness value 0.7-2.0 mmol/l (70- 200 mg/l CaCO3)
The use of soft water is strictly forbidden!
Please consult a water specialist!
3.7.1 Drain Cooling
The feed water supplied to the drain cooling must meet the following
requirements:
•Hardness: 0.7 - 0.2 mmol/l.
•Water temperature shall not exceed 15°C (59°F).

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3.8 Specification
Property
Value
Overall dimensions
Height
980mm
Width
540mm
Length
450mm
Maximum dimensions
(door open)
Height
1300mm
Overall dimensions
(WR configuration)
Height
1047mm
Width
540mm
Length
434mm
Maximum dimensions
(door open)
Height
1376
Net weight
101kg.
Shipping volume
0.56 m3
Shipping weight
115 kg
Shipping dimensions
length
730mm
width
610mm
height
125mm
Max. Allowable Working pressure
(MAWP)
2.8 bar
Chamber
diameter
280mm
Depth
400mm
Chamber Volume
31L

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3.9 Construction
Chamber and door material
Stainless steel
Outer Cabinet
Stainless Steel
Chamber insulation
Fiberglass with reinforced material
3.10 Utilities
Power Supply
Power
1 Ph, 230V/50/60Hz
Recommended
Circuit Breaker
16A
Compressed Air (LVC only)
1/2" 3 Bar (44-58 psi)
Tap water
1/2", 2-6 Bar (29-44 psi)
Mineral free water
1/2", 2-3 Bar (29-44 psi)
Drain
2" Minimum Withstanding temp. of 80 M
(17676)
Attention!
•A switch or circuit-breaker must be included in the building
installation. This switch or circuit-breaker shall be in close
proximity to the equipment, within easy reach of the operator; and
marked as the disconnecting device for the equipment.
•The electrical net must be protected with a current leakage safety
relay.
•The electrical network must comply with local rules or
regulations.
•Verify that there is an easy access to the main power switch, to
the water cut-off valve and to the current leakage safety relay.
•Make sure while placing the autoclave, to leave space around the
machine, to give the technician access to service the machine.
•All water connections to autoclave must be performed through
"BACK FLOW PREVENTION SYSTEM" only, as per IEC 61770.

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3.11 Symbol Description
Caution! Consult accompanying documents
Caution! Hot surface.
Caution! Hot steam.
Protective earth (Ground)
On-Off

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3.12 Front View
No.
Description
No.
Description
1
Door handle
9
Printer
2
Door cover
10
N/A
3
USB socket
11
Right service door
4
Display
12
Right service door grip
5
Keyboard
13
Front leg
6
Main switch
14
Front service cover
7
Printer cover
15
Ventilation grill
4
5
15
2
1
3
6
9
10
13
12
14
11
7
8

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8
Right service door lock

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3.12.1 Front View: What is Special for WR Configuration
No.
Description
No.
Description
1
Glass tube indicator of the
reservoir water level
3
Inlet for manual water
reservoir filling
2
Wheel
2
1
2