Varian ProStar 701 Release note

Varian, Inc.
2700 Mitchell Drive
Walnut Creek, CA 94598-1675/USA
©Varian, Inc. 2005 03-914947-27:Rev.2
ProStar 701 Fraction
Collector Control
Galaxie Driver Manual

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Table of Contents
Introduction.............................................................................................. 2
General................................................................................................................................2
Installation and Configuration................................................................ 3
Installing the Driver Software...............................................................................................3
Configuring the Communication Server...............................................................................3
Configuring the Driver..........................................................................................................4
Configuring the Varian ProStar 701 Fraction Collector.................................................4
Configuring the Synopsis..............................................................................................5
Building a Method ................................................................................... 7
Creating a New Method.......................................................................................................7
Control Method Section for the Varian ProStar 701 Fraction Collector.............................10
Collector Parameters Control......................................................................................12
Fraction Collector Mode Control.................................................................................14
Rack Control ...............................................................................................................17
Collection Chain Control .............................................................................................19
Starting and Viewing an Acquisition.................................................... 21
Quick Start and Sequence.................................................................................................21
Quick Start (Single Acquisition) ..................................................................................21
Sequence....................................................................................................................23
Running Acquisition Status................................................................................................24
Viewing a Running Acquisition....................................................................................24
Status of the Varian ProStar 701 Fraction Collector...................................................24

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Introduction
General
This document describes the configuration of the Varian
ProStar 701 Fraction Collector driver. It also explains how to
build a control method for this driver, how to start and view a
running acquisition and what actions are available during an
analysis.

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Installation and
Configuration
Installing the Driver Software
To install the Galaxie Drivers software refer to the Galaxie Data
System Installation Guide.
Configuring the Communication Server
To communicate with the ProStar 701 module an
RS232_Interface or an RS232_PC communication bus must be
configured on the acquisition server.
The parameters of the bus to must be the following:
Stops 1 Stop
Baud 19200 Parity EVEN
Bits 8 Bits Flow Control NONE
Buffer Tx 4096 Buffer Rx 4096
NOTE: For more details about the configuration of an RS232_Interface or an RS232_PC
bus, refer to the Galaxie Chromatography Data System Installation’s Guide.

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Configuring the Driver
The driver software configuration is done in the Galaxie
Configuration Manager when a new chromatographic system is
created.
Configuring the Varian ProStar 701 Fraction Collector
Click on the button corresponding to the Varian ProStar
701 Fraction Collector driver and the configuration screen
appears.
Communication Window
Select from the Hardware Interface list the name of the RS232
Bus where the system has been connected.
NOTE: For more details about the RS232 Bus configuration, refer to the
Configuring the Communication Server section in this manual.

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Define the number of ProStar 701 Fraction Collector installed in
the Number of instrument spin edit. Up to four ProStar 701
Fraction Collectors can be defined.
For each fraction collector, define the address in the Fraction
Collector X ID field. Then press the
button, to connect to the instrument.
Configuring the Synopsis
In the Overview part of the system configuration, a synopsis can
be defined for the system. This synopsis depends on the
configured devices.
1. To save the configuration of these devices, click on the OK
button.
2. Right click on the system name in the bottom part of the
Galaxie Configuration Manager screen. A pop-up menu
appears: click on Stop. Then right click on the system name
again, and click on Start.
3. Go back to the System Configuration screen (Properties
wizard). A graphic of the devices that comprise the system
is displayed in the synopsis screen. To configure the
synopsis as the real instrument configuration, move each
module, and then connect it appropriately. To do this, select
one anchor for each of the two modules (the selected
anchors become red) and click on the button; a
connection tube will appear between the two anchors.
Click on the to delete a selected tube, click on the
button to delete all the tubes.
Click on to define a background color, or on to
make the background transparent.
Click on the icon to change the grid color.

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4. Once the synopsis configuration is complete, it will be
displayed in the Galaxie Chromatography Data System, in
the Systems tab. In this tab, the Overview part will show the
synopsis of the system with some parameters displayed
(temperature, flow, signal, etc.) depending on the installed
devices.
For example, the following pictures represent the synopsis
of the Varian ProStar 701 Fraction Collector:
Galaxie Configuration Manager Screen
Galaxie Chromatography Data System Screen
NOTE: Once the system has been created and correctly configured, it must be
associated with one or more projects in order to start some acquisitions. For
more details about the creation and configuration of a system, refer to the
Galaxie Configuration Manager User’s Guide.
Click on the button to print the current configuration.

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Building a Method
Creating a New Method
The method contains parameters for instrument control, data
acquisition, chromatogram processing, and editing of final
results. To initially set up a method, it is not necessary to define
all of the sub-method sections; however, you must fill in the
instrument control section prior to starting an acquisition.
1. To create a new method, select the FILE / NEW METHOD
option from the Galaxie Chromatography Data System main
menu.
A wizard guide will appear and provide assistance during
the first steps of method creation:

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2. Choose the system (chromatograph) associated with the
method. A method is created for a particular system. When
starting an acquisition, the name of the system that
performs the acquisition must be correct, since method
access is limited only to those methods associated with this
system.
3. Once this field is completed, click on the Next button to
move to the second step of the method creation:

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4. Enter the New Method Name in the first field. In the
Description field, enter information concerning the method
(optional).
5. Once these two fields have been completed, click on the
OK button. The new method is created and is opened with
default parameters in the Galaxie Chromatography Data
System. The next step is the definition of each of the
method sections.
The instrument control section must be defined in order to
start an acquisition. In the following part of this guide, only
the control section for the Varian ProStar 701 Fraction
Collector is detailed. Other parameters such as Injection
Volume, Vial and Rack number may be necessary and are
defined in the acquisition section.
NOTE: For more details about the programming of each method section, turn to the
Galaxie Chromatography Data System User’s Guide.

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Varian ProStar 701 Fraction Collector Control
Method
Once the new method is created, select the control section in
the lower part of the browser.
The following screen appears:
Press the button to display the System Control Method
Advanced Tools window:
to send the current method to the instrument.
to get the active method from the instrument.
to print the current method.

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Click on the button to display the ProStar 701 Fraction
Collector control method section. It contains four sub-sections:
Collector Parameters, Fraction Collector Mode, Racks, and
Collection Chain. Click on the required sub-section to define the
corresponding parameters that will be used during the
chromatographic run. The toolbar allows you to easily scroll
through the control method windows:
to select the first window of the control method.
to move back to the previous window of the control
method.
to move forward to the next window of the control
method.
to select the final window of the control method.

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Collector Parameters Control
Collector Parameters Parameter Group:
Select from the Mode list the way the fraction size is defined:
None: no collection
Time: fraction size based on time
Drop: fraction collection by drop
Note that some parameters of the other sub-sections will be
activated or may change according to the entry.
Select in the Pattern list weather the fraction collector uses the
serpentine or the unidirectional mode to collect samples.

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Serpentine Unidirectional
Define in the Flow delay field the time delay (in seconds)
between when a peak is sensed and when the collection valve
is activated. This option is available only if Slope or Level
options are checked in the Fraction Collection Mode sub-
section. The values run from 0 to 3559 sec.
Define in the Collection Option list where the peaks and non-
peaks are sent:
Peaks =Rack1&2; Non-peaks= Valve
Peaks =Rack1&2; Non-peaks= Drain
Peak/Non-peaks=Consecutive tubes
Uploaded.
The Uploaded entry is the entry that is entered into the front
panel of the fraction collector. This allows the use of collection
options that cannot be sent to the fraction collector. If this is
selected, the collection option in the fraction collector will be
used.

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Fraction Collector Mode Control
Fraction Collector Mode Parameter Group:
In the Sense Peak By options, define the way the presence of
peaks will be determined:
Time: peaks are determined by time. The Fraction
Windows table is activated. The user has to determine
the time size of each fraction.
Slope: peaks are sensed by their rate of change of
signal. As a peak starts rising, it will be sensed and
collection will be started. The peak separation is based
on the range in the slope of the baseline over a wide
range of user defined peak widths. If selecting this

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option, complete the three fields Slope sensitivity, Peak
Width and Detector Signal.
Level: peaks are sensed by their absolute level above
baseline. This option does not involve the collection of
the entire peak, but only of the part that rises above the
threshold level (defined in the Threshold Level field). This
mode of collection allows collecting only the most
concentrated portion of the peak. Collection of peaks is
based on percentage of full scale height. If 0% is defined
in the Threshold level field, the Level feature is canceled.
If selecting this option, complete the two fields Detector
Signal and Threshold Level.
User can combine Slope or Level collection modes with Time by
using logical AND Time or OR Time. If AND Time is selected,
the peak will only be collected when both a time window is
active, and the slope or level detection conditions are met. If OR
Time is selected, the peak will be collected if either the time
condition or the slope or level conditions are met.
If Slope and Level are selected together, they are always linked
with the AND logical operator.
Slope sensitivity: define the range of sensitivity, two choices are
available: Normal and High.The Normal sensitivity requires a
larger slope change before indicating a peak. The High
sensitivity should be used with smooth baselines to collect the
maximum sample of broad based peaks or smaller peaks.
Peak Width: it is the expected average peak width at baseline.
Values are proposed in the scrolling list.
Detector Signal: it is the detector output value. Three choices
are available: 10mV, 100mV and 1 V.
Threshold level: it is the percentage (0 to 100%) of the full scale
height since which peaks are collected.

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Fraction Windows: this table has to be filled if the Time mode of
collection is activated. User has to define the fraction time sizes.
Up to 25 lines can be defined.
Use the following buttons to edit the table:
to add a line to the table.
to delete a line in the table.
to clear the entire table.

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Rack Control
Rack X Parameter Group:
Define the rack(s) parameters. Two racks can be defined.
According to the chosen rack in the RackSize list, the
Tubes/Rack field is automatically filled in.
The value displayed by default in the Tubes/Rack field is the
maximum tubes available in the rack; this value can be reduced
by the user.
In the Fraction Size field enter the maximum number of seconds
or drops (depending on whether Time or Drop mode is defined
in the Collector Parameters screen) that can be contained in a
tube. If this value is exceeded, the fraction collector moves to

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another tube. The peak is then divided into several fractions (1
fraction =1 tube).
Rack size Max tube number Comments
No rack 0
12/13 mm tubes 144
16 mm tubes 100
18 mm tubes 72
17 mm vials 72
25/28 mm vials 36
Microvials 60
Prep funnel 26 not available for Rack 2
Bottles 9
Beaker 1
Custom 256
Use of a rectangular rack with max 16 rows
and 16 tubes per row. User has to define
the first and last tube position, ProStar 701
Fraction Collector will calculate the row
and column spacing. The configuration is
done from the ProStar 701 Fraction
Collector instrument.
Random 50 Allow user to program up to 50 tube
positions per rack in any sequence and
position. The configuration is done from the
ProStar 701 Fraction Collector instrument.
Microplate 192

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Collection Chain Control
Collection Chain Parameter Group:
This sub-section allows the user to overlay collections in same
tubes. For example to concentrate peaks, user can inject
several similar samples via a sequence of injections, and collect
fractions in same tubes: all the fractions 1 of the X samples are
sent in the same tube, all the fractions 2 of the X samples are
sent in the same tube…(where X is the Overlay Number).
In the Overlay Number field, define the number of time the
collector drops solution in the tube. If 3 is defined, that means
that three successive injections will be distributed in the same
tubes, because the same fractions are defined for the three
injected samples. To use the Collection Chain option, it is