VWR symphony 5.3 A User manual

Valid: 06.2009/ 50111308 B
Operating Instructions
CO2Incubator symphony 5.3 A
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Copyright
These operating instructions are protected by copyright. Rights resulting
thereof, particularly reprint, photomechanical or digital postprocessing or
reproduction, even in part, are only allowed with the written consent of the
VWR.
This regulation does not apply to reproductions for in-plant use.
Trademarks
All trademarks mentioned in the operating instructions are the exclusive
property of the respective manufacturers.
VWR International
1310 Goshen Parkway
West Chester, PA 19380
USA
Technical Support
When contacting the Technical Support, please have the following information
available:
• Model Number,
• Serial Number,
• Date Purchased.
Phone [phone]

Operating Instructions | symphony 5.3 A – 06/2009
Contents
3
1 Important safety informations 7
1.1 Instruction of the operating personnel.................................................................. 7
1.2 Applicability of the instructions ............................................................................. 7
1.3 Warranty ................................................................................................................. 7
1.4 Explanation of Signs .............................................................................................. 8
1.4.1 Symbols and signal words used in the operating instructions...................... 8
1.4.2 Pictographs used in the operating instructions .............................................. 8
1.4.3 Signs on the device.......................................................................................... 9
1.5 Overview safety informations................................................................................ 9
1.6 Intended purpose of the device........................................................................... 12
1.6.1 Correct use ..................................................................................................... 12
1.6.2 Incorrect use................................................................................................... 12
1.6.3 Standards and directives ............................................................................... 13
2 Delivery 14
2.1 Packaging ............................................................................................................. 14
2.2 Components standard equipment....................................................................... 14
2.3 Acceptance inspection......................................................................................... 14
3 Installation of the device 15
3.1 Ambient conditions.............................................................................................. 15
3.2 Room ventilation .................................................................................................. 15
3.3 Space requirements............................................................................................. 16
3.4 Transport .............................................................................................................. 16
3.5 Stacking ................................................................................................................ 17
3.6 Modifications ........................................................................................................ 17
4 Description of the device 18
4.1 Views of device .................................................................................................... 18
4.2 Safety devices ...................................................................................................... 20
4.3 Work space atmosphere ...................................................................................... 20
4.4 Sensor system...................................................................................................... 21
4.5 Door switch .......................................................................................................... 21
4.6 Switchbox with supply interface ......................................................................... 22
4.7 Work space components ..................................................................................... 23
4.8 90 °C decontamination routine (optional) ........................................................... 25
5 Start-up 26
5.1 Preparing the work space .................................................................................... 26
5.2 Installing the shelf system ................................................................................... 26
5.3 Gas connection..................................................................................................... 28
5.4 Power supply connection .................................................................................... 29
5.5 RS 232 interface connection................................................................................ 29
5.6 Connecting the alarm contact.............................................................................. 32

Operating Instructions | symphony 5.3 A – 06/2009
Contents
4
6 Operation 34
6.1 Preparing the device ............................................................................................ 34
6.2 Starting operation ................................................................................................ 34
7 Handling and control 36
7.1 Power switch ........................................................................................................ 36
7.2 Operating panel.................................................................................................... 36
7.3 Control self-test .................................................................................................... 38
7.4 Setting the nominal temperature value............................................................... 39
7.5 Setting the CO2nominal value ............................................................................ 39
7.6 Activating the auto-start routine.......................................................................... 40
7.7 Reading failure codes........................................................................................... 41
7.8 Resetting the thermal protection......................................................................... 45
8 Shut-down 46
8.1 Shutting the device down.................................................................................... 46
8.2 Turn device off using the power switch.............................................................. 46
9 Cleaning and disinfection 47
9.1 Decontamination procedures .............................................................................. 47
9.2 Wipe/Spray disinfection....................................................................................... 47
9.3 90 °C decontamination routine (optional) ........................................................... 50
9.3.1 90 °C decontamination routine procedure.................................................... 51
9.3.2 Activating the 90 °C decontamination routine .............................................. 52
9.3.3 Cancelling the 90 °C decontamination routine ............................................. 52
10 Maintenance 53
10.1 Inspections and checks...................................................................................... 53
10.2 Service intervals ................................................................................................. 53
10.3 Preparing the temperature calibration .............................................................. 53
10.4 Temperature calibration procedure .................................................................. 55
10.5 Preparing the CO2calibration............................................................................ 56
10.6 CO2calibration procedure ................................................................................. 57
10.7 Replacing the sterile filters................................................................................. 58
10.8 Replacing the device fuses ................................................................................ 58
10.9 Replacing the door seal ..................................................................................... 59
11 Parts and accessories 60
11.1 Lists of parts and accessories............................................................................ 60
12 Technical data 61
13 Disposal 64

Operating Instructions | symphony 5.3 A – 06/2009
Contents
5
14 Device log 66
15 Certificate of decontamination 67

Figures
6
Operating Instructions | symphony 5.3 A – 06/2009
Fig.1: Device dimensions ...................................................................................... 16
Fig.2: Lift point ....................................................................................................... 16
Fig.3: Stacking........................................................................................................ 17
Fig.4: General views of device .............................................................................. 19
Fig.5: Temperature sensor .................................................................................... 21
Fig.6: Door switch.................................................................................................. 21
Fig.7: Supply interfaces ......................................................................................... 22
Fig.8: Work space layout / Water reservoir .......................................................... 23
Fig.9: Rear panel openings.................................................................................... 24
Fig.10: Support rail installation/removal................................................................. 26
Fig.11: Shelf support/sheet insert installation ........................................................ 27
Fig.12: Gas pressure hose installation.................................................................... 28
Fig.13: Power supply connection............................................................................ 29
Fig.14: RS 232 interface........................................................................................... 29
Fig.15: Example of a connection scheme............................................................... 33
Fig.16: Power switch / Operating panel.................................................................. 37
Fig.17: Removing blower wheel and cover............................................................ 49
Fig.18: 90 °C decontamination routine procedure ................................................. 51
Fig.19: Temperature calibration .............................................................................. 54
Fig.20: CO2calibration............................................................................................. 56
Fig.21: Sterile filter replacement ............................................................................. 58
Fig.22: Device fuse replacement............................................................................. 58
Fig.23: Door seal replacement ................................................................................ 59
Fig.24: CO2consumption ........................................................................................ 63

Operating Instructions | symphony 5.3 A – 06/2009
7
1 Important safety informations
1.1 Instruction of the operating personnel
These operating instructions describe the CO2 incubator
Model • Model symphony 5.3 A.
The CO2 incubator has been manufactured in keeping with the latest tech-
nological developments and has been tested before delivery for its correct
function. It may, however, present potential hazards if it is not used accor-
ding to the intended purpose or outside of operating parameters. There-
fore, the following procedures must always be observed to prevent
accidents:
•TheCO
2 incubator must be operated only by trained and authorized
personnel.
Written instructions • For any operation of this device, the operator must prepare clear and
concise written instructions in the language of the operating and cle-
aning personnel based on these operating instructions, applicable
safety data sheets, plant hygiene guidelines, and technical regulations,
in particular:
– which decontamination measures are to be applied for the CO2
incubator and accessories,
– which protective measures apply while specific agents are used,
– which measures are to be taken in the case of an accident.
• Repairs to the device must be carried out only by trained and authori-
zed expert personnel.
1.2 Applicability of the instructions
The contents of the operating instructions are subject to change without
further notice.
Basic language • Concerning translations into foreign languages, the US version of these
operating instructions is binding.
• Keep these operating instructions close to the device so that safety
instructions and important information are always accessible.
• Should you encounter problems that are not detailed adequately in
these operating instructions, please contact VWR immediately for your
own safety.
1.3 Warranty
VWR warrants the operational safety and functions of the CO2 incubator
only under the condition that:
• the device is operated and serviced exclusively in accordance with its
intended purpose and as described in these operating instructions,
• the device is not modified,
• only original spare parts and accessories that have been approved by
VWR are used,
• inspections and maintenance are performed at the specified intervals.
Begin of warranty The warranty is valid from the date of delivery of the device to the operator.

Operating Instructions | symphony 5.3 A – 06/2009
1 Important safety informations1 Important safety informations
8
1.4 Explanation of Signs
1.4.1 Symbols and signal words used in the operating instructions
1WARNING
Indicates a hazardous situation which, if not avoided, could result in
death or serious injury!
1CAUTION
Indicates a hazardous situation which, if not avoided, could result in
minor or moderate injury!
NOTICE
Indicates a situation which, if not avoided, could result in property
damage!
NOTE
Is used for applicational hints and useful information.
1.4.2 Pictographs used in the operating instructions
Pictographs point to concrete facts of a safety message or a property
damage message.
Electric shock!
Suffocation hazard!
Hot surfaces!
Wear safety gloves!
Wear safety goggles!!
Harmful liquids!

Operating Instructions | symphony 5.3 A – 06/2009
1 Important safety informations1 Important safety informations
9
1.4.3 Signs on the device
Saftey signs, test marks and marks of conformity placed on the device.
Observe operating instructions!
CSA test mark:
Test certificate for USA/Canada, indicates that the product has met the
requirements of nationally recognised product safety standards.
1.5 Overview safety informations
Overview of he most important safety informations used in this operating
instructions.
Carbon dioxide (CO2):
As CO2is rated as a harmful gas, certain safety instructions must be obser-
ved when the CO2 incubator is started up and when the device is operated.
NOTE!
Personnel instruction:
Personnel operating devices with CO2supply must be instruc-
ted about the particularities in the handling of CO2before star-
ting their work:
• Correct operation of pressurized gas containers and gas
supply systems,
• Obligation to report damages and shortcomings in CO2sup-
ply lines,
• Measures to be taken in case of accidents or failures.
• These instructions must be repeated in appropriate intervals
and must comprise the particular operating instructions of
the gas supplier.
1WARNING
Suffocation hazard:
CO2released in large amounts into the room
atmosphere may cause suffocation.
If CO2is released, initiate safety measures
immediately!
• Leave the room immediately and do not allow others to
enter the room!
• Inform security service or fire department!

Operating Instructions | symphony 5.3 A – 06/2009
1 Important safety informations1 Important safety informations
10
NOTICE!
Overpressure:
The operating pressure of the gas applied to the device must
not exceed 1 bar. If the gas is supplied at a higher pressure, the
valves integral to the device may not close correctly and the
gas supply control may be impaired!
• Set the gas supply to a range between 0.8 bar min. and 1.0
bar max. and make sure that this pressure setting cannot be
changed!
Pressure compensation opening:
To ensure permanent pressure compensation, the pressure
compensation opening must not be connected to an exhaust
air system. The pipe of the pressure compensation opening
must not be extended or redirected!
Access port:
• If the access port is not used, it must be capped during ope-
ration!
Gas quality:
CO2must be of the following qualities:
• Purity 99.5 % min,
• medical grade.
1CAUTION
Electric shock:
Contact with current-carrying components may cause a lethal
electric shock.
Before connecting the device to the power supply, check plug
and connection line for damage.
Do not use damaged components when connecting the device
to the power supply!
Health hazard:
The surfaces of the work space may be contaminated. Contact
with contaminated cleaning liquids may cause infections. Disin-
fectants may contain harmful substances.
When cleaning and disinfecting, always observe the safety
instructions and hygiene regulations!
• Wear safety gloves.
• Wear safety glasses.
• Wear mouth and respiratory system protection gear to pro-
tect the mucous membranes.

Operating Instructions | symphony 5.3 A – 06/2009
1 Important safety informations1 Important safety informations
11
• Observe the safety instructions of the manufacturer of the
disinfectant and of the hygiene experts!
Contamination hazard:
The device can be used for preparing and processing infectious
substances so that the device or device components may
become contaminated.
• In case of a shut-down, the device must be decontaminated!
Before device components are discarded, they must be decon-
taminated!
• The device components must be cleaned thoroughly; after
the cleaning, they must be disinfected or sterilized, as requi-
red by the application.
• Discarded devices or device components must be provided
with an appropriate certificate showing the decontamina-
tion measures performed!
Hygiene regulations:
Prior to any operation, the user must clean and disinfect the
work space in accordance with the hygiene regulations set
forth by the operator to protect the cultures.
NOTICE!
Incompatible cleaning agents:
Some device components are made of plastic. Solvents may
dissolve plastics. Powerful acids or bases may cause embrittle-
ment of the plastics.
For cleaning the plastic components and surfaces, do not use
hydrocarbon-containing solvents, detergents with an alcohol
content of more than 10 % or powerful acids and bases!
Chloride-containing disinfectants:
Chloride-containing disinfectants may corrode stainless steel.
Use only disinfectants that do not affect stainless steel!
• Do not use sodiumhypochlorite solutions such as Purex and
Clorox. These can cause corrosion and pitting of stainless
steel.
• Do not use steel wood pads such as Brillo. These pads depo-
sit carbon particles in the work space.
Alcoholic disinfectants:
Disinfectants with an alcohol content of more than 10 % may
form, in combination with air, easily combustible and explosive
gas mixtures.

Operating Instructions | symphony 5.3 A – 06/2009
1 Important safety informations1 Important safety informations
12
When using such disinfectants, avoid open flames or exposure
to excessive heat during the entire disinfection process!
• Use such disinfectants only in adequately ventilated rooms.
• After the disinfectant has been allowed to react, wipe the
cleaned device components thoroughly dry!
• Observe safety regulations to avoid fire and/or explosion
hazard caused by alcohol-containing disinfectants!
Water supply:
The water tray of the work space can hold up to 3.0 l of proces-
sed water. When the running operation, always keep a suf-
ficient quantity of processed water of the following quality
available:
• demineralized and distilled or autoclaved for sterilization,
or
• completely deionized and distilled or autoclaved for sterili-
zation.
• When the water level falls below the lower limit, refill
water!
1.6 Intended purpose of the device
1.6.1 Correct use
The CO2 incubator is a laboratory device for preparing and cultivating cell
and tissue cultures. The device allows the simulation of the special physio-
logical ambient conditions for these cultures due to the exact control of:
Control features • temperature,
•CO
2content,
• an increased relative humidity.
The device has been designed for installation and operation in the follo-
wing fields of application:
Fields of application • Laboratories for cytobiological and biotechnological experiments of
safety levels L1, L2, and L3.
• Medical-microbiological laboratories in accordance with DIN 58 956.
• Laboratories in the central area of clinics and hospitals.
Gas supply The CO2required for the incubator is supplied to the device from a separate
gas supply system, either from gas cylinders or from a central pressurized
gas container.
The layout of the gas supply system must ensure that the operating pres-
sure of the gas supply lines can be set to a range between 0.8 bar (min.) to
1 bar (max.) and that the pressure cannot be changed.
Depending on the capability of the gas supply system, several devices may
be connected to one gas cylinder.
The CO2 incubator is suited for continuous operation.
1.6.2 Incorrect use
Safety levels Do not use cell or tissue cultures in the device that are not in accordance
with the regulations of safety levels L1, L2, and L3.

Operating Instructions | symphony 5.3 A – 06/2009
1 Important safety informations1 Important safety informations
13
Do not use tissues, substances or liquids that:
• are easily ignitible or explosive,
• release vapors that form combustible or explosive mixtures when
exposed to air,
• release poisons.
1.6.3 Standards and directives
The device is in accordance with the following standards and guidelines:
Standards • CSA – C22.2 No. 1010.1-92
• CSA – C22.2 No. 1010.1-B97
• EN 61010 – 1:2001
• EN 61010 – 2-10:2003
Safety specifications:
Specifications • Indoor Use Only
• Altitude – Up to 2.000 meters
• Temperature – 5 °C to 43 °C
• Humidity – Maximum RH 80 % for temperatures up to 31 °C, decreasing
linearly to 50 % RH at 40 °C
• Mains Supply Fluctuations – Mains supply voltage fluctuations not to
exceed ±10 % of the nominal voltage
• Installation Category II1
• Pollution Degree 22
• Class of Equipment I
1Installation category (overvoltage category) defines the level of transient overvoltage which
the instrument is designed to withstand safely. It depends on the nature of the electricity sup-
ply and is overvoltage protection means. For example, in Cat II which is the category used for
instruments in installations supplied from a supply comparable to public mains such a hospital
and research laboratories and most industrial laboratories, the expected transient overvoltage
is 2500V for a 230V supply and 1500V for a 120V supply.
2Pollution degree describes the amount of conductive pollution present in the operating envi-
ronment. Pollution degree 2 assumes that normally only non-conductive pollution such a dust
occurs with the exception of occasional conductivity caused by condensation.

Operating Instructions | symphony 5.3 A – 06/2009
14
2Delivery
2.1 Packaging
Packaging materials The CO2incubator symphony 5.3 A is delivered in a stable packaging box.
All packaging materials can be separated and are reusable:
• Packaging carton: Recycled paper
• Foam elements: Styrofoam (CFC-free)
• Pallet: Untreated wood
• Packaging film: Polyethylene
• Packaging ribbons: Polypropylene
2.2 Components standard equipment
Standard equipment
2.3 Acceptance inspection
Delivery check After the device has been delivered, check the delivery immediately for:
• completeness,
• possible damages.
If damages are detected or if components are missing, please contact the
carrier and vendor immediately.
Delivery
CO2incubator with
single glass door and with
continuous shelves
(standard version)
Shelf 3
Shelf support post 4
Shelf support rail 6
Insert for pressure
compensation opening 1
Plug for pipe channel 1
Power supply cable 1
Connector, potential-free
contact 1
Spare caps, set 1
CO2connecting hose set 1
Immersion water pump 1
Open-end wrench,
24 mm 1
Allen wrench 2 mm for blower
wheel 1
Allen wrench 3 mm for blower
wheel cover 1
Operating instructions 1

Operating Instructions | symphony 5.3 A – 06/2009
15
3 Installation of the device
3.1 Ambient conditions
The device must only be operated in locations that meet the particular
ambient conditions listed below.
Requirements:
• Draft-free and dry location.
• The minimal distance to adjacent surfaces must be observed on all
sides (see Section 3.3.).
• The operating room must be equipped with appropriate room ventila-
tion.
• Solid, level, fire-proof surface.
• Vibration-proof substructure (floor stand, lab table) capable of bearing
the weight of the device and accessories (particularly if several devices
are stacked).
• To ensure a constant incubation temperature of 37 °C, the ambient tem-
perature must be within a range of +18 °C to +33 °C.
• Relative humidity up to 80 % (max.).
• Avoid direct exposure to sunlight.
• Devices that produce excessive heat should not be used near the loca-
tion of the CO2 incubator symphony 5.3 A.
3.2 Room ventilation
When CO2is supplied, the work space of the incubator is slightly pressuri-
zed. The pressure is released through the pressure compensation opening
into the operating room.
As the pressure compensation and any opening of the glass door/gas tight
screen during the operation of the device will release very small quantities
of CO2into the operating room, the room ventilation must be capable of
carrying the released gas safely off into the open.
In addition, heat dissipating from the device during permanent operation
may cause a change in the room climate.
• Therefore, the incubator symphony 5.3 A must only be installed in
rooms with sufficient ventilation.
• Do not install the device in room recesses without ventilation.
• The room ventilation should be a technical ventilation that is in accor-
dance with the requirements of ZH 1/119 (Guidelines for laboratories)
or some other suitable ventilation system with appropriate capacity.

3 Installation of the device
Operating Instructions | symphony 5.3 A – 06/2009
3 Installation of the device3 Installation of the device
16
3.3 Space requirements
AFig.1: When installing the device, make sure that the
installation and supply connections are freely accessi-
ble.
The control box at the rear panel of the device may
serve as a spacer to adjacent objects. The side
distances given are minimal distances.
To protect the CO2incubator against contamination,
use a floor stand even if the device is installed near the
floor. The height of the floor stand should not be less
than 200 mm.
Several floor stands and carriers are available as opti-
ons from VWR (part numbers see Section 11.1, “Parts
and accessories”).
NOTE!
Accessibility of the devices:
To ensure the accessibility for care and
maintenance works, keep larger side and
rear distances!
3.4 Transport
Fig.2: For transport do not lift the device using the
doors or components attached to the device (e.g. con-
trol box on rear panel) as lift points.
NOTE!
Lift points:
Lift the device only using the lift points
shown in Fig. 2!
x
75
100
100
669
870
637
766
F
F
F
³185
Fig.1: Device dimensions
Fig.2: Lift point

3 Installation of the device
Operating Instructions | symphony 5.3 A – 06/2009
3 Installation of the device3 Installation of the device
17
3.5 Stacking
AFig.3: Two symphony 5.3 A devices can be stacked
on top of each other. The stacking elements [1] and [2]
secure the devices on top of each other.
If the devices are placed onto mobile racks, ensure
that the rollers [3] are secured by an arresting device
during operation. For reasons of stability, the rollers
should be oriented to the front.
NOTE!
Transporting stacked devices:
The stacking elements are anot connecting
elements. Therefore, the transport of stak-
ked devices on sloped surfaces is not allo-
wed!
3.6 Modifications
NOTE!
Modifications:
Retrofits and modifications must only be
performed by the Technical Service of
VWR!
3
21
Fig.3: Stacking

Operating Instructions | symphony 5.3 A – 06/2009
18
4 Description of the device
4.1 Views of device
Front view:
Description front side [1] Stacking elements
[2] Plug caps
[3] Glass door
[4] Measuring cell with blower wheel and sensors
[5] Door switch
[6] Pressure compensation opening with insert
[7] Measuring opening
[8] Outer door
[9] Door handle
[10] Outer door seal, replaceable
[11] Stand, height-adjustable
[12] Nameplate
[13] Power switch
[14] Support rail
[15] Shelf
[16] Latch, glass door
[17] Support hook for shelf
[18] Access port with plug
[19] Glass door seal, replaceable
Rear view
Description rear side [20] Stacking elements
[21] Pressure compensation opening
[22] Access port, Ø 42 mm
[23] Switchbox with supply interfaces
[24] CO2gas container

Operating Instructions | symphony 5.3 A – 06/2009
4 Description of the device4 Description of the device4 Description of the device
19
12
21011
21
23
20
20
20
22
24
13
14
16
17
18
19
15
25 431 8
76 9
Fig.4: General views of device

Operating Instructions | symphony 5.3 A – 06/2009
4 Description of the device4 Description of the device
20
4.2 Safety devices
Safety features The device is equipped with the following safety devices:
• A door switch interrupts the CO2supply and the work space heating
when the glass door is opened.
• An independent thermal protection protects the samples from harmful
overheating in the event of a failure.
• A pressure compensation opening ensures pressure compensation in
the device work space.
• Audible and visual alarms indicate failures during operation.
4.3 Work space atmosphere
In the work space of the incubator, the particular physiological ambient
conditions for the preparation and cultivation of cell and tissue cultures are
simulated. The work space atmosphere is determined by the following fac-
tors:
Physiological ambient conditions • Temperature,
• Relative humidity,
•CO
2concentration.
Temperature:
To ensure undisturbed operation, the temperature in the operating room
must be at least 18 °C and the incubation temperature must be at least 3 °C
higher than the room temperature.
The heating system controls the incubation temperature from this tempe-
rature threshold up to 55 °C. The principle of air jacket heating and the addi-
tional, separate heating of the outer door minimize the risk of condensate
formation on the side walls, at the ceiling of the work space, and at the
glass door.
Relative humidity:
The water tray of the work space can hold 4.5 liters of processed water. The
heating of the work space causes evaporation of the water, thereby ensu-
ring a constant humidity within the work space. Under normal operating
conditions and at the usual incubation temperature of 37 °C, a constant
relative humidity of approx 95 % is achieved in the work space.
If heated containers are removed and put back into the work space, the ele-
vated humidity and the cooling may cause condensate to form on the outer
sides of the container.
Water quality For humidification, processed water of the following quality is required:
• demineralized and either distilled or autoclaved for sterilization,
or
• completely deionized and either distilled or autoclaved for sterilization.
CO2supply:
To ensure correct growth conditions for the cell and tissue cultures, the
work space is supplied with CO2. The pH of the bicarbonate-buffered cul-
ture media largely depends on the CO2content of the work space