VWR VWR51014991 User manual

Operating Instructions
VWR Air Jacketed CO2Incubator


VWR International
Preface
Air Jacketed CO2 Incubator i
*T/C is a thermal conductivity sensor.
IR is an infra-red sensor.
**Dual stack
MANUAL NUMBER 751014991
0 40591 1/12/16 Original ccs
REV ECR/ECN DATE DESCRIPTION By
VWR Catalogue
Number
Manufacturer
Model Number Sensor* Capacity (cu ft) Voltage
10810-902 VWR51014991 T/C 6.5 115V/60Hz
10810-944 VWR51014992 IR 6.5 115V/60Hz
10811-002 VWR50114902 T/C 6.5 X 2** 115V/60Hz
10811-004 VWR50114903 IR 6.5 x 2** 115V/60Hz
Legal Address of Manufacturer
United States
VWR International, LLC
100 Matsonford Rd
Radnor, PA 19087
800-932-5000
http://www.vwr.com
Country of origin: United States

VWR Internationalii Air Jacketed CO2 Incubator
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. VWR International makes no representations or warranties with respect to this
manual. In no event shall VWR *OUFSOBUJPOBMbe held liable for any damages, direct or incidental, arising out of
PSrelated to the use of this manual.
Intended Use:
The VWR Air Jacketed CO2 Incubators are designed to maintain an optimal environment for the incubation of
tissue and cell samples. These models are designed to maintain temperature and carbon dioxide levels as set by
the operator as well as monitoring chamber relative humidity. CO2 is controlled using either a thermo-
conductivity cell, or an infra-red sensor.
The VWR Air Jacketed Incubators are approved for general purpose use only.
Non-intended Use:
The VWR Air Jacketed Incubators are not intended for use where electrical or physical contact with the patient
is established.
These incubators are not intended to be operated in potentially explosive environments and are not intended for
use with flammable materials.
The VWR Air Jacketed Incubators are not intended for use as a Microbiological Incubator (21 CFR 866.2540)
and are also not approved for use in assisted reproductive procedures for the incubation of ova and embryos (21
CFR 884.6120).
Warning If the incubator is not used in the manner specified in this operating manual, the protection provided
by the equipment design may be impaired. s
©2015 VWR International. All rights reserved.

VWR International Air Jacketed CO2 Incubator iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

VWR Internationaliv Air Jacketed CO2 Incubator
Preface
Do You Need Information or Assistance on
VWR International Products?
VWR can provide information on pricing and give you quotations. We can take your order and
provide delivery information on major equipment items or make arrangements to have your local sales
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Visit VWR's website at vwr.com for:
• Complete technical service contact information
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For information or technical assistance, contact your local VWR representative, or visit vwr.com.
VWR can supply technical information about proper setup, operation or troubleshooting of your
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Whatever VWR products you need or use, we will be happy to discuss your applications. If you
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chances are, correct it yourself...over the telephone without a service call.
When more extensive service is necessary, we will assist you with direct factory trained
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the VWR International warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.

Air Jacketed CO2 Incubator vVWR International
Table of Contents
Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Keypad Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3
Displays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Installing the Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4
Stacking the Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Duct Sheets and Shelf Installation . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Access Port Filter & CO2 Sensor Cover Plate . . . . . . . . . . . . . . . . .1-9
Air Sample Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
HEPA Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Leveling the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Electrical Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Filling the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11
Connecting the CO2 Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . .1-12
Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-13
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Thermal Conductivity CO2 System . . . . . . . . . . . . . . . . . . . . . . . .2-2
Infrared CO2 System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-3
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Turn Audible Alarm ON/OFF . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
New HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Set REPLACE HEPA filter reminder . . . . . . . . . . . . . . . . . . . . . . .3-2
Set Access Code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Enable Low Temp Alarm to Trip Contacts . . . . . . . . . . . . . . . . . . .3-3
Set Low Temp Alarm Limit (tracking alarm) . . . . . . . . . . . . . . . . .3-3
Set High CO2 Alarm Limit (tracking alarm) . . . . . . . . . . . . . . . . .3-4
Set Low CO2 Alarm Limit (tracking alarm) . . . . . . . . . . . . . . . . .3-4
Set New Zero Number for T/C CO2 Sensors . . . . . . . . . . . . . . . .3-5
Enable CO2 Alarms to Trip Contacts . . . . . . . . . . . . . . . . . . . . . . .3-5
Set New Span Number for T/C CO2 Sensors . . . . . . . . . . . . . . . .3-6
Section 1
Section 2
Section 3

Section 6
Section 8
Section 7
Section 9
vi Air Jacketed CO2 Incubator VWR International
Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Temp Controller Failure TMP CNTR ERR . . . . . . . . . . . . . . . . . .4-2
Sensor Fault Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Clean Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Replace Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
HEPA Filter Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Clean Glass Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Clean Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Replace Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Electronics Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
Sterilization Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Factory Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Remote Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Customer Installed Accessories and Parts . . . . . . . . . . . . . . . . . . . . . .8-1
Exploded Part Drawing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2
Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Table of Contents
Section 4
Section 5

Air Jacketed CO2 Incubator 1-1VWR International
Section 1 Installation and Start-Up
• Chamber Gas Sample Port - Used for sampling chamber CO2content
using a FYRITE or similar instrument.
• Main Power Switch - Mains Disconnect
• Control Panel - Keypad, displays & indicators (Figure 1-2)
• Leveling Legs - Used to level the unit
• Sterilization Cycle Button - Switch to initiate sterilization cycle
Note The incubators are stackable. Information follows. s
Figure 1-1. Components
Sterilization Cycle
button
Control panel
Door heater cable See Section 5.
See
Section 1.
Main power switch
Chamber gas sample port
Inner door
Leveling legs (4)

Figure 1-2. Control Panel
1-2 Air Jacketed CO2 Incubator VWR International
Section 1
Installation and Start-Up
Silence Switch: Press to mute audible alarm. See Section 4 for alarm
ringback times.
Visual Alarm Indicator - Lights pulses on/off during an alarm condition.
MODE Select - Used to select Run, Setpoints, Calibration and
Configuration modes.
Message Display - Shows system status.
Mode Select Indicators -
RUN: Run Menu
SET: Set Points Menu
CAL: Calibration Menu
CON: Configuration Menu
HEAT Indicator - Lights when heaters are activated.
Injection INJ Indicator - Lights during CO2injection into chamber.
Temp [T (°C)] Display - Programmable to display temperature. See
Configuration section.
CO2[CO2 (%)] Display - Programmable to display CO2percentage. See
Configuration section.
RUN SET CAL CON
MODE
HEAT
INJ
T (°C)
CO (%)
2
SCROLL FOR PARAMETERS
ENTER
Mute
Mode
Select
Visual
Alarm Indicator
Mode Select Indicators
Heat Indicator
Injection Indicator
Temp Display
CO Display
2
Up/Down
Arrows
Scroll for
Parameters Arrows
Enter
Message Display

Air Jacketed CO2 Incubator 1-3VWR International
Section 1
Installation and Start-Up
The VWR Air Jacketed Incubator has four basic modes for incubator
setup: Run, Setpoints, Calibration and Configuration.
• Run is the default mode for normal operation.
• Set is used to enter system setpoints for incubator operation.
• Calibration is used to calibrate various system parameters.
• Configuration allows for custom setup of various options.
Table 1-1. Selections Under Each Mode
Scroll Keys: Steps the operator through parameters of SET, CAL and
CON modes. The right arrow goes to the next parameter, the left arrow
returns to the previous parameter.
Up/Down Keys: Increases/decreases or toggles the parameter value select-
ed in SET, CAL, and CON modes.
ENTER: Must press this key to save-to-memory all changed values.
Keypad Operation
RUN SETPOINT CALIBRATION CONFIGURATION
Default Mode Temperature Temp Offset Audible
Overtemp CO2Cal1New HEPA Timer
CO2IR Cal2Replace HEPA Reminder
Access Code
Temp Lo Alarm
Temp Relay
CO2Lo Alarm
CO2Hi Alarm
CO2Relay
CO2Z & S #'s *
Display Temp
Display RH
1T/C units Base Unit Displays
2IR units Option Unit Displays
*T/C units only

Installing the
Incubator
Message Display: Shows system status (Mode) at all times. Shows ‘CLASS
100’ or ‘SYSTEM OK’ during normal operation, or alarm messages if
the system detects an alarm condition (see Section 4, Alarms). The mes-
sage ‘CLASS 100’ is a timing mechanism indicating that, under normal
operating conditions with the HEPA filter installed, the air inside the
chamber meets the Class 100 air cleanliness standard for particulates of
0.5 micron size or larger per cubic foot of air.
Upper and Lower Displays: The 7 segment upper display shows tempera-
ture. The lower display shows CO2.
1. Maintain a minimum three inch clearance behind the incubator for
electrical and gas hook-ups. In addition, a three inch ventilation space
is needed on each side.
2. Locate the unit on a firm level surface capable of supporting the unit
weight of 260 lbs (118kg).
3. Locate the unit away from doors and windows and heating and air
conditioning ducts.
4. Lift the unit by the sides of the cabinet base. Do not attempt to lift it
by the front and back. This places stress on the outer door hinges.
Warning Install stacked units against a wall or similar structure. s
Warning With incubators in a stacked configuration, do not leave both
exterior doors open at the same time. s
Warning If the units have been in operation, turn them both off and
disconnect the power source before beginning any service work. s
Two stacking brackets (shown at left) are included in the
parts bag shipped with each incubator.
1-4 Air Jacketed CO2 Incubator VWR International
Section 1
Installation and Start-Up
Displays
Figure 1-3. Stacking Brackets
Stacking the
Incubators

Stacking Incubators
(continued)
1. Remove the cover plate securing the door cord from the incubator to
be on top. See Figure 1-4. Disconnect the plug from the connector.
2. Remove the four screws securing the door hinges to the unit. Remove
the door and set it aside.
3. Unscrew the two hole plugs from the top cover of the incubator to be
the bottom of the stack (Figure 1-5).
4. Unscrew and remove the 4 leveling feet from
the unit to be stacked on top and lift it onto
the bottom unit. Align all sides.
Warning This incubator weighs 260 lbs (118kg).
Have sufficient personnel available when lifting.
Lift the unit by the sides of the cabinet base to
avoid placing stress on the outer door hinge. s
5. Insert the stacking brackets into the slots at the
back of the stacked units as shown in Figure 1-
7.
6. Align the slotted holes in the brackets with the
mounting holes on the back of the top
incubator. Secure the brackets with the screws
and washers provided in the parts bag. See Figure 1-8.
Air Jacketed CO2 Incubator 1-5VWR International
Section 1
Installation and Start-Up
Figure 1-4. Cover Plate
Figure 1-6. Stacked
Cover plate and bolt hole
with door connector
Figure 1-5. Hole Plug Locations
Figure 1-8. Installed Brackets on Back of Unit
Stacking
bracket
Stacking
bracket
Bottom incubator back
Top incubator back
Top unit
Bottom unit
Figure 1-7. Bracket
into Slot

7. Thread one 1/4 x 20 bolt and washer
included with the stacking brackets,
into the hole behind the cover plate.
Do not tighten. Refer to Figure 1-9.
8. Remove the cover plate from the same
area on the other side of the top unit.
9. Thread the other 1/4 x 20 bolt and washer into this hole.
10. Tighten the bolts on both sides.
11. Assemble the door hinges to the unit. Secure with the screws.
12. Plug the door cord into the connector, as previously. Secure the cover
plate.
13. Install the cover plate on the other side of the unit. The stacked
incubators are ready for service.
1. Remove vinyl from shelf channels, duct sheets, and air duct, if present.
2. Using a suitable laboratory disinfectant, clean all interior surfaces.
Caution Before using any cleaning or decontamination method except
those recommended by the manufacturer, users should check with the
manufacturer that the proposed method will not damage the equipment.
Accidental spills of hazardous materials on or inside this unit are the
responsibility of the user. s
1. Installed included grommets into the back
flange of each duct sheet; 6 grommets per sheet.
See Figure 1-10.
2. Install the side ducts with the tabs facing into
the center of the chamber with their slots up.
There are no right side or left side ducts, simply
rotate one of them to fit the opposite side. Tilt
the side ducts as they are placed into the
chamber so the tops fit into the top air duct,
then guide them into the vertical position.
Figure 1-11 shows the side duct as it would be
oriented for the right side of the chamber.
1-6 Air Jacketed CO2 Incubator VWR International
Section 1
Installation and Start-Up
Cover plate and bolt hole
with door connector
Figure 1-9. Bolt Hole
Preliminary Cleaning
Stacking Incubators
(continued)
Figure 1-10. Grommets
on back of duct sheet
Duct Sheet and Shelf
Installation

3. Referring to Figure 1-11, note that there is no difference in the left
and right shelf channels.
4. Install the shelf channels by placing the channel’s rear slot over the
appropriate rear tab on the side duct. Pull the shelf channel forward
and engage the channel’s front slot into the side duct’s appropriate
forward tab. Refer to Figure 1-12.
5. Figure 1-11 shows one of the channels installed on the right side duct.
6. Locate the supplied rod, spring and end pieces; 4 each. Assemble the
spring to the rod by positioning the very end of the spring over the
ridge at the rod. Then press the spring to the opposite side. It should
snap into place. See Figures 1-13 and 1-14.
7. Slide end cap over spring (Figure 1-15).
Note When installing the rods, the
spring end can be installed on either
side of the chamber. The upper front
rod is high in the chamber and the
upper back rod is lower to allow access
to the access port filter door.
Air Jacketed CO2 Incubator 1-7VWR International
Section 1
Installation and Start-Up
Duct Sheet and Shelf
Installation (continued)
Side Duct
Side toward side duct
Side toward shelf
Right Side Duct
with ShelfChannel Installed
Shelf Channels
Side duct
tab
Shelfchannel
rear slot
Shelfchannel
front slot
Side duct
tab
Figure 1-11. Shelf Channels & Side Ducts Figure 1-12. Channels and Slots
Figure 1-13. Spring End
Spring
end
Ridge
Figure 1-15. Spring End
Figure 1-14. Spring Assemble to Rod

1-8 Air Jacketed CO2 Incubator VWR International
Section 1
Installation and Start-Up
8. Install one end of the rod into the appropriate hole in the duct sheet.
9. Compress the spring (under
the end cap) to insert the
other end of the rod into
the hole in the duct sheet
on the opposite side.
10. Figure 1-16 shows the four
rods installed.
Note Using the supplied spring
rods will ensure the side ducts
are the proper distance needed
for shelves to move freely. s
1. Locate the opening in the top left corner of the interior chamber.
Remove the tape from the opening on the outside of the unit.
2. Locate the stopper with filter in the hardware bag. Lift the metal port
cover and install the assembly in the opening inside the chamber. See
Figure 1-17.
3. Also in the hardware bag is the CO2 sensor cover plate. Install, using
the 1/4-turn fasteners. Refer to Figure 1-17 for the location.
Installing Duct Sheets
and Shelves (cont.)
Back wall of chamber
Front opening of chamber
Figure 1-16. Four Rods Installed in Chamber
Access Port Filter &
CO2Sensor Cover Plate
Air Sample
Filter
Access
Port Filter
and stopper
assembly
HEPA
Filter
Thumbscrews
CO Sensor
2
Cover Plate
Figure 1-17. Filter and Plate

Air Jacketed CO2 Incubator 1-9VWR International
Section 1
Installation ans Start-Up
Air Sample Filter
Installation
1. Remove the filter from the
shipping bag.
2. Install the air sample filter
assembly to the black hose
barb behind the top duct.
3. Insert the other end of the
filter assembly onto the
metal tubing on the top
duct. Refer to the Figure
1-18.
Caution Be careful when the filter. The media can be damaged if it is
mishandled. To avoid damage to the incubator, do not operate the unit
without the HEPA filter in place. s
1. Remove the filter from the shipping box.
2. Remove the plastic coating from the filter, using caution not to touch
the filter media.
3. Install the filter as shown in Figure 1-18. Refer to Section 5 for HEPA
filter maintenance.
Caution The media of the filter can be damaged if mishandled.
To avoid damage to the incubator, do not operate the unit without the
HEPA filter in place. s
Check the unit for being level by placing a bubble-style level on one of the
shelves. Turn the hex nut on the leveler counterclockwise to lengthen the
leg or clockwise to shorten it. Level the unit front-to-back and left-to-
right.
See the serial tag on the side of the unit for electrical specifications or refer
to the electrical schematics at the end of this manual.
Caution Serial tag rating is based on amperage draw during sterilization
cycle. Normal operating amperage is much less. Ensure that electrical
circuit will handle amp draw of sterilization cycle.
Connect the incubator to a grounded, dedicated circuit. The power cord
connector is the mains disconnect device for the incubator. Position the
unit so that it can be easily disconnected. s
Air Sample
Filter
metal tubing
hose
barb
behind
top duct
HEPA
collar
Figure 1-18. Air Sample Filter Installation
HEPA Filter
Installation
Electrical Power
Connection
Leveling the Unit

Plug the provided power cord into the power inlet connector on the back
of the cabinet (Figure 1-19), then into the grounded, dedicated, electrical
circuit.
Fill the humidity pan with sterile distilled water to within 1/2” of the top.
Place the pan directly in the center of the incubator floor.
For applications requiring high humidity, the pan should be placed against
the left side wall of the incubator. The side ducts have been modified to
allow the pan to be placed against the wall. Optimum humidity is achieved
by capping the CO2sample port. This will, however, cause condensation
in the chamber. To enhance RH recovery from door openings, place a
second humidity pan in the right side duct.
For best operation of the incubator, sterilized distilled, demineralized or
de-ionized water should be used in the humidity pan. Water purity should
be in the resistance range of 50K to 1M Ohm/cm, or a conductivity range
of 20.0 to 1.0 uS/cm. Refer to ASTM Standard D5391-93 or D4195-88
for measuring water purity.
Distillation systems, as well as some types of reverse osmosis water purity
systems, can produce water in the quality range specified. Tap water is not
recommended as it may contain chlorine, which can deteriorate the
stainless steel. Tap water may also have a high mineral content, which
would produce a build-up of scale in the pan. High purity or ultra pure
water is not recommended as it is an extremely aggressive solvent and will
deteriorate the stainless steel.
1-10 Air Jacketed CO2 Incubator VWR International
Section 1
Installation and Start-Up
Filling the Humidity
Pan
ACCESSORY
75 WATTS MAX.
RS 485
ALARM CONTACTS
30V MAX / 1A MAX
#2
CO inject
15 p.s.i. Regulated
(103.4 kPa)
#1
CO inject
15 p.s.i. Regulated
(103.4 kPa)
22
aRJ-ll telephone style connectors
IR CO units only
b
2
CO Inlet2
#1 Tank
aRemote
Alarm Contacts
30V max/1A max
bAir
Intake
Power Cord
Connector
Accessory
Outlet
75 watts max
Figure 1-19. Back of Cabinet
Electrical Power
Connection (cont.)

Air Jacketed CO2 Incubator 1-11VWR International
Section 1
Installation and Start-Up
High purity water has a resistance of above 1M to 18M Ohm. Even high
purity water can contain bacteria and organic contaminants. Water should
always be sterilized or treated with a decontaminant, safe for use with
stainless steel as well as safe for the product, prior to being introduced into
the humidity pan.
Check the level and change the water frequently to avoid contamination.
Do not allow the water level to fluctuate significantly. “Dry-outs” will have
an adverse effect on the humidity level and CO2calibration of the T/C
units.
Caution Use of chlorinated water, or decontamination products containing
chlorine, will deteriorate the stainless steel and cause rust, voiding the
warranty. s
Warning High concentrations of CO2gas can cause asphyxiation! OSHA
Standards specify that employee exposure to carbon dioxide in any eight-
hour shift of a 40-hour work week shall not exceed the eight-hour time
weighted average of 5000 PPM (0.5% CO2). The short term exposure
limit for 15 minutes or less is 30,000 ppm (3% CO2). Carbon dioxide
monitors are recommended for confined areas where concentrations of
carbon dioxide gas can accumulate. s
Warning This incubator is designed to be operated with CO2gas only.
Connecting a flammable or toxic gas can result in a hazardous condition.
Gases other than CO2should not be connected to this equipment. CO2
gas cylinders have a UN1013 label on the cylinder and are equipped with a
CGA 320 outlet valve. Check the gas cylinder for the proper identification
labels. The CO2gas supply being connected to the incubator should be
industrial grade, 99.5% pure. Do not use CO2gas cylinders equipped with
siphon tubes. A siphon tube is used to extract liquid CO2 from the
cylinder which can damage the pressure regulator. Consult with your gas
supplier to ensure that the CO2cylinder does not contain a siphon tube.
Gas cylinders should also be secured to a wall or other stationary object to
prevent them from tipping.
A two-stage CO2pressure regulator is required to be installed on the outlet
valve of the gas cylinder. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa) for proper performance of the CO2
control system. A single stage CO2pressure regulator will not maintain 15
psig (103.4 kPa) to the incubator as the pressure in the CO2cylinder
decreases; therefore, a two stage regulator is recommended.
Connecting the CO2
Gas Supply
Filling the Humidity
Pan (continued)

Warning If higher purity CO2is desired inside the incubator (greater than
99.5% pure), the pressure regulator should be constructed with a stainless
steel diaphragm, along with specifying the purity of the CO2from the gas
supplier. Follow the manufacturer’s instructions to ensure proper and safe
installation of the pressure regulator on the gas cylinder.
Consult your facility safety officer to ensure that the equipment is installed
in accordance with the codes and regulations that are applicable in your
area. s
The CO2gas supply being connected should be industrial grade 99.5%
pure and should not contain siphon tubes. Install a two-stage pressure
regulator at the cylinder outlet. The high pressure gauge at the tank should
have 0-2000 psig range. The low pressure gauge, at the incubator inlet,
should have a 0-30 psig range. Input pressure to the incubator must be
maintained at 15 psig (103.4 kPa).
The incubator has serrated fittings on the back of the cabinet to connect
the gas supply. Refer to Figure 1-19. The fitting is labeled CO2 Inlet #1
Tank. Make sure that the connections are secured with clamps. Check all
fittings for leaks.
With the incubator properly installed and connected to power, the
humidity pan filled, and the unit connected to gas supplies, system
setpoints can be entered. The following setpoints can be entered in SET
mode: Temperature, Overtemperature and CO2. To enter SET mode,
press the MODE key until the SET indicator lights. Press the right
and/or left arrow keys until the proper parameter appears in the message
display. See Chart 1-1 for more detail.
All VWR Air Jacketed Incubators have an operating temperature range of
10°C to 50°C, depending on ambient temperature. The incubator is
shipped from the factory with a temperature setpoint of 10°C. At this
setting, all heaters are turned off.
To change the operating temperature setpoint:
1. Press the MODE key until the SET indicator lights.
2. Press the right arrow until “Temp XX.X” is displayed in the message
display.
3. Press the up/down arrow key until the desired temperature setpoint is
displayed.
1-12 Air Jacketed CO2 Incubator VWR International
Section 1
Installation and Start-Up
Incubator Start-Up
Setting the Operating
Temperature
Connecting CO2 Gas
Supply (continued)

Air Jacketed CO2 Incubator 1-13VWR International
Section 1
Installation and Start-Up
4. Press ENTER to save the setpoint.
5. Press the MODE key until the RUN indicator lights for RUN mode
or press the right/left arrow keys to go to next/previous parameter.
Caution The independent overtemp system is designed as a safety to
protect the incubator only. It is not intended to protect or limit the
maximum temperature of the cell cultures or customer’s equipment inside
the incubator if an overtemp condition occurs. s
VWR Air Jacketed Incubators are equipped with a secondary temperature
monitoring system to monitor the air temperature inside the cabinet. This
system is designed as a safety device to turn off all heaters in the event of a
temperature control failure. Temperature control in the incubator will be
±1° of the overtemp setpoint.
The overtemperature is set by the factory (default) at 40°C. However, the
overtemp can be set up to 55°C in 0.5° increments.
If the incubator’s operating temperature setpoint is set above the overtemp
setpoint, the overtemp setpoint will automatically update to 1°C above the
temperature setpoint. It is recommended that the overtemp setpoint be
maintained at 1°C over the operating temperature setpoint.
To set the Overtemp setpoint:
1. Press the MODE key until the SET indicator lights
2. Press the right arrow until Otemp XX.X is displayed in the message
display
3. Press the up or down arrow key until the desired Overtemp setpoint is
displayed
4. Press ENTER to save the setting
5. Press the MODE key until the RUN indicator lights or press the right
or left arrow to go to the next or previous parameter.
Setting the Operating
Temperature (continued)
Setting the Overtemp
Setpoint