Optika Italy B-150P Series User manual

Model
B-150P-MRPL
B-150P-BRPL
B-150P Series
INSTRUCTION MANUAL
Ver. 5.2 2022

Page 2
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 B-150P-MRPL 4
3.2 B-150P-BRPL 4
4. Unpacking 5
5. Intended use 5
6. Symbols and conventions 5
7. Instrument description 6
7.1 B-150P-MRPL 6
7.2 B-150P-BRPL 7
8. Assembling 8
9. Use of the microscope 9
9.1 Light intensity adjustment 9
9.2 Coarse focus tension adjustment 9
9.3 Stage 9
9.4 Adjust the interpupillary distance 9
9.5 Diopter adjustment 10
9.6 Aperture diaphragm 10
9.7 Use with rechargeable batteries 10
9.8 Useinbrighteld 11
9.9 Use in polarized light 11
10. Maintenance 12
11. Troubleshooting 13
Equipment disposal 14
Table of Contents

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4
3. Package content
3.1 B-150P-MRPL
3.2 B-150P-BRPL
① Frame
② Monocularobservationhead
③ Eyepiece
④ Tensionadjustmenttool
⑤ Objectives(4X/10X/40X)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupply
③
④
⑤
⑥⑦⑧
①
②
① Frame
② Monocularobservationhead
③ Eyepiece
④ Tensionadjustmenttool
⑤ Objectives(4X/10X/40X)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupply
③
④
⑤
⑥⑦
⑧
①
②

Page 5
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 6
7. Instrument description
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
EYEPIECE
OBSERVATION
HEAD
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
STAGE
CLIPS
POLARIZER
ANALYZER
7.1 B-150P-MRPL

Page 7
STAGE
CLIPS
7.2 B-150P-BRPL
LED CHARGE
INDICATOR
FINE FOCUS
KNOB
STAGE
OBSERVATION
HEAD
CONDENSER
OBJECTIVES
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
NOSEPIECE
EYEPIECES
POLARIZER
ANALYZER
COARSE
FOCUS KNOB
TENSION
ADJUSTMENT
COLLAR

Page 8
8. Assembling
1. Remove the dust cap from the microscope frame and from
the bottom of the observation head.
2. Insert the optical head above the stand and tighten the screw.
(Fig. 1)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3
Insert both eyepieces into the tubes of the optical head. (Fig. 2)
• For model B-150P-MRPL: only one eyepiece is used as
this model is monocular.
3. Insert the power supply jack in the socket placed at the rear
side of the microscope. (Fig. 3)

Page 9
9. Use of the microscope
FFig. 6ig. 6
FFig. 4ig. 4
①
FFig. 5ig. 5
②
FFig. 7ig. 7
③
④
⑤
9.1 Light intensity adjustment
Operate on the light intensity adjustment dial ① to turn ON /
OFF the microscope and to increase / decrease the illumination
voltage. (Fig. 4)
9.2 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The tension of the coarse focusing knob is factory preset.
1. To modify the tension according to personal’s needs, rotate
the ring ② . (Fig. 5)
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go lower by itself or
the focus easily lost after ne adjustment. In this case, rotate
the knob in order to increase the tension .
9.3 Stage
Stage is equipped with two clips ③ for the sample placement.
Standard specimen is glass slide, thickness 1,2 mm with cover-
slide 0,17mm. (Fig. 6)
Specimen can be moved with hands.
1. Lift the clip, place the specimen and gently release the clip
until the specimen is secured over the stage.(Fig. 6)
2. When used in polarized light stage can be rotated on 360°,
be grabbing the knurled ring ④ placed on the outer side of
the stage.
9.4 Adjust the interpupillary distance
Only B-150P-BRPL
Hold the right and left parts of the observation head using both
hands and adjust the interpupillary distance by turning the two
parts until one circle of light can be seen. (Fig. 7)
• The graduation on the interpupillary distance indicator ⑤,
pointed by the spot “.” on the eyepiece holder, shows the
distance between the operator’s eyes.
The range of the interpupillary distance is 48- 75 mm.

Page 10
9.5 Diopter adjustment
Only B-150P-BRPL
1. Look into the right eyepiece with your right eye only, and fo-
cus on the specimen.
2. Look into the left eyepiece with your left eye only. If the image
is not sharp, use the dioptric adjustment ring ①to compen-
sate. (Fig. 8)
• The adjustment range is ±5 diopter. The number indicat-
ed on the adjustment ring graduation should correspond
to the operator’s dioptric correction. FFig. 8ig. 8
FFig. 9ig. 9
②
FFig. 10ig. 10
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
FFig. 11ig. 11
③
①
9.6 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aperture dia-
phragm a󰀨ects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of fo-
cus of the image. Move the diaphragm lever ② (Fig. 9) to-
ward left or right to decrease or increase the N.A. value.
• With low contrast specimens set the numerical aperture to
about 70%-80% of the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty sleeve, adjust the con-
denser’s diaphragm in order to obtain an image like the one
in Fig. 10
9.7 Use with rechargeable batteries
When the microscope is plugged with the power supply, the LED
indicator for the battery recharge ③ is lit. (Fig. 14)
• Power supply connected, low battery: led turns RED. When
charging is finished, it turns GREEN.
• Power supply not connected, microscope off: LED is always
OFF.
• Power supply not connected, microscope on, battery
charged: LED is GREEN.
• Power supply not connected, microscope on, battery almost
empty: LED is RED.