Optika Italy B-380 Series User manual

Model
B-382PL-ALC
B-383PL
B-382PLI-ALC
B-383PLI
B-382PH-ALC
B-383PH
B-382PHI-ALC
B-383PHI
B-383FL
B-383LD1
B-383LD2
B-380 Series
INSTRUCTION MANUAL
Ver. 4.0 2019

Pagina 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-382PL-ALC / B-382PLI-ALC 4
5.2 B-383PL / B-383PLI 5
5.3 B-382PH-ALC / B-382PHI-ALC 6
5.4 B-383PH / B-383PHI 7
5.5 B-383FL 8
5.6 B-383LD1 / B-383LD2 10
6. Unpacking 11
7. Assembling 11
7.1 B-382PL-ALC / B-382PLI-ALC 11
7.2 B-383PL / B-383PLI 12
7.3 B-382PH-ALC / B-382PHI-ALC 12
7.4 B-383PH / B-383PHI 13
7.5 B-383LD1 / B-383LD2 13
7.6 B-383FL 14
7.7 Assembling the microscope 15
8. Summaryofbrighteldobservationprocedures 17
9. Use of the microscope 18
9.1 Light intensity adjustment 18
9.2 Adjust the interpupillary distance 18
9.3 Diopter adjustment 18
9.4 Coarse focus tension adjustment 18
9.5 Focus lock lever 19
9.6 Stage 19
9.7 Condenser centering 19
9.7.1 Centeringwithoutelddiaphragm 19
9.7.2 Centeringwithelddiaphragm 20
9.8 E󰀨ectsoftheelddiaphragm 20
9.9 Aperture diaphragm 20
9.10 Use of oil immersion objective 21
9.11 Use of ALC system 21
10. Useofuniversalcondenserforbrighteld/darkeld/phasecontrast 22
10.1 Brighteldobservation(BF) 22
10.2 Darkeldobservation(DF) 22
10.3 Phasecontrastobservation(PH) 23
10.4 Useofthegreenlter 24
11. Microphotography 24
11.1 Installing the C-mount adapter 24
11.2 Useofreexcameras 24
12. Useinuorescence 25
12.1 Assemblingprocedure(allmodels) 25
12.2 HBObulbassembling(B-383FL) 25
12.3 Centering the HBO bulb (B-383FL) 27
12.4 Useofthemicroscope(B-383FL) 29
12.5 Useofthemicroscope(B-383LD1/LD2) 29
12.6 Use of the shutter 29
12.7 Useofthelightexcludingplate 30
13. Fluorescenceobservationprocedures(B-383FL) 31
14. Fluorescenceobservationprocedures(B-383LD1/LD2) 31
15. SimultaneousobservationPhaseContrast+Fluorescence(B-383FL) 32
16. Maintenance 32
17. Troubleshooting 33
Equipment disposal 35
Summary

Pagina 3
1. Warning
                




2. Symbols and conventions

CAUTION

ELECTRICAL SHOCK

3. Safety Information
Avoiding Electrical Shock

󰀨



4. Intended use


Pagina 4
5. Overview
5.1 B-382PL-ALC / B-382PLI-ALC

PHOTO/TV PORT











DIAL



























Pagina 5
5.2 B-383PL / B-383PLI

PHOTO/TV PORT






DIAL

























Page 6
5.3 B-382PH-ALC / B-382PHI-ALC

PHOTO/TV PORT











DIAL



























Page 7
5.4 B-383PH / B-383PHI

PHOTO/TV PORT






DIAL

























Page 8
5.5 B-383FL









DIAL















Page 9
PHOTO/TV PORT

LAMP














B-383FL(OPPOSITESIDE)

Page 10
5.6 B-383LD1 / B-383LD2



DIAL
















PHOTO/TV PORT


















Page 11
6. Unpacking
              
                  -
               
                  
 
 
7. Assembling
:
7.1 B-382PL-ALC / B-382PLI-ALC
① Frame
② Objectives
③ ALC observation head
④ Eyepieces
⑤ Tension adjustment tool
⑥ Dust cover
⑦ Power supply
⑧ Immersion oil
①
③
④
⑤
⑥⑦
⑧
②

Page 12
7.2 B-383PL / B-383PLI
7.3 B-382PH-ALC / B-382PHI-ALC
① Frame
② Objectives
③ Trinocular observation head
④ Eyepieces
⑤ Tension adjustment tool
⑥ Dust cover
⑦ Power supply
⑧ Immersion oil
⑨ Photo tube
①
③
④
⑤
⑥⑦
⑧
②
⑨
① Frame
② Objectives
③ ALC observation head
④ Eyepieces
⑤ Tension adjustment tool
⑥ Dust cover
⑦ Power supply
⑧ Immersion oil
⑨ Green lter + lter holder
⑩ Centering telescope
①
③
④
⑤
⑥⑦
⑧
②
⑨
⑩

Pagina 13
7.4 B-383PH / B-383PHI
7.5 B-383LD1 / B-383LD2
① Frame
② Objectives
③ Trinocular observation head
④ Eyepieces
⑤ Tension adjustment tool
⑥ Dust cover
⑦ Power supply
⑧ Immersion oil
⑨ Photo tube
⑩ Green lter + lter holder
⑪ Centering telescope
①
③
④
⑤
⑥⑦
⑧
②
⑨
⑩
⑪
① Frame
② Objectives
③ Trinocular observation head
④ LED uorescence illuminator
⑤ Eyepieces
⑥ Photo tube
⑦ Dust cover
⑧ Immersion oil
⑨ Tension adjustment tool
⑩ Power supply
⑪ Light excluding plate
①
③
④⑤⑥
⑦
⑧②
⑨⑩
⑪

Pagina 14
7.6 B-383FL
① Frame
② Objectives
③ Trinocular observation head
④ HBO uorescence illuminator
⑤ Fluorescence power supply + power cord
⑥ Eyepieces
⑦ Photo tube
⑧ Immersion oil
⑨ Tension adjustment tool
⑩ Dust cover
⑪ Power supply
⑫ Light excluding plate
⑬ HBO mercury bulb
①
③
④
⑤⑥
⑦
⑧②
⑨
⑩⑬⑫
⑪

Pagina 15
Fig. 1
7.7 Assembling the microscope
1. 

ALC models only
2. 

3.     -
       
4.       
    

Fig. 2
Fig. 3
Fig. 4

Pagina 16
FieldDiaphragm(Optional)
1. 

• It may take a little bit of force to unscrew the
lens.
2. 
3. 
Fig. 5

Pagina 17
8. Summaryofbrighteldobservationprocedures


( 

 

D

 

  


 
 







specimen




9.1
9.6
5
5
5
5
9.2
9.3
9.7
9.9
9.7
9.1

Pagina 18
9. Use of the microscope
9.1 Light intensity adjustment
① 
-

• Only for B-383LD1 / B-383LD2: the switch lo-
cated at the back of the microscope operates
toturnonthetransmittedlight(position“I”)
or the reected light (position “II”). Turn on
the microscope for transmitted light by tur-
ningtheswitchto“I”.
9.2 Adjust the interpupillary distance

-


9.3 Diopter adjustment
1. 

2.         
      
②
-
arers.
      

9.4 Coarse focus tension adjustment

rotate the ring ③      

 


to increase the tension.
Fig. 6
①
Fig. 7
Fig. 8
②
Fig. 9
③

Pagina 19
9.5 Focus lock lever

       

1.  ①


2.        

3.         


     󰀨  

• To unlock, move the knob in the opposite
direction to the one used for the lock.
9.6 Stage



stage.
1. ②

2. 
• A sudden release of the the spring arm could
cause the falling of the slide.
9.7 Condenser centering
9.7.1 Centeringwithoutelddiaphragm



         

        

1. 
-

2. 
3. ③


4. 
④

5. 
⑤ 


6. 
Fig. 10
①
Fig. 11
②
Fig. 12
⑤
③
④

Pagina 20
9.7.2 Centeringwithelddiaphragm
1. -

2. ①

3. ②

4.     ③to bring the


5. 
-

6. -

9.8 E󰀨ectsoftheelddiaphragm

a high contrast image.

-

9.9 Aperture diaphragm

󰀨


  ①   


I-
-

       
      
    -

        
one in Fig. 16.
Fig. 15
①
Fig. 16




Fig. 13
①
Fig. 14
②
③