Zeiss Axiocam MR Installation and operating manual

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Number of this manual: M 60-3-0014 e
Printed 12.2008

Carl Zeiss draws the User's attention to the fact that the information and references
contained in these documents may be subject to technical modifications, in particular
due to the continuous further development of Carl Zeiss's products. The documents
enclosed do not contain any warranty by Carl Zeiss with regard to the technical
processes described in the documentation or to certain reproduced product
characteristics. Furthermore, Carl Zeiss shall not be held liable for any possible printing
errors or other inaccuracies in this documentation, unless proof can be furnished that
any such errors or inaccuracies are already known by Carl Zeiss or that these are not
known to Carl Zeiss due to gross negligence and that furthermore Carl Zeiss has for
these reasons refrained from eliminating these errors or inaccuracies appropriately.
Carl Zeiss hereby explicitly draws the User's attention to the fact that this manual only
contains a general description of the technical processes and information, the
implementation of which in any individual case may not be appropriate in the form
described here. In cases of doubt, we recommend the User to consult Carl Zeiss.
This manual is protected by copyright. Carl Zeiss has reserved all rights to this
documentation. It is prohibited to make copies, partial copies, or to translate this
manual into any other language, except copies for personal use.
Carl Zeiss explicitly draws attention to the fact that the information contained in this
manual will be updated regularly in compliance with the technical modifications and
supplements carried out in the products and furthermore that this manual only reflects
the technical status of Carl Zeiss's products at the time of printing.
Copyright 2005-2008 by Carl Zeiss
Microsoft, Windows, Windows XP Professional and Windows Vista are registered
trademarks of Microsoft Corporation in the United States and/or other countries.
FireWire is a trademark of Apple Computer, Inc.
i.Link is a registered trademark of Sony Corporation in Japan and/or other countries.
Carl Zeiss MicroImaging GmbH
07740 Jena, Germany
BioSciences | Göttingen Location
Phone: [phone]
Telefax: [phone]
E-Mail: [email protected]
www.zeiss.de

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The referenced Software of Carl Zeiss ("Zeiss Software"), contained in this
documentation, was designed, realized, verificated, validated and released in
a certificated process environment. The quality management system is
certified following the rule of DIN EN ISO 9001.
The fields of application of the Software are common tasks and applications in
microscopy respectively imaging (so called "Off-The-Shelf Software"). Though
the user acknowledges that in any kind of use the end user of the Software is
responsible for the validation of the Software for the end user’s dedicated
intend of use considering all requirements of law and standards (e. g. 21 CFR
part 11, FDA, MDD etc.). If necessary the end user has to establish, to
document, to implement and to maintain a special process to fulfill all the
requirements to be conform with the validate rules of law and standards.
CARL ZEISS DOES NOT WARRANT THAT THIS SOFTWARE IS USABLE FOR
SPECIAL PURPOSES OTHER THAN IN THE FIELDS OF APPLICATION DEFINED
ABOVE.
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Refer to the safety notes and instructions in the manuals of all
necessary devices (e.g. microscope peripherals, cameras,
computers, computer additionals, etc.) before installing and
using the software.

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This product has been developed, tested and manufactured in
accordance with the applicable environmental provisions and
directives of the European Union.
The product and its accessories comply with EU directives 2002/95/EC
(RoHS) and 2002/96/EC (WEEE), insofar as these apply to this product.
We have implemented a take-back and recycling process that ensures
that proper recycling is carried out in accordance with the
aforementioned EU directives.
Please contact your Carl Zeiss sales/service organization for details relating
to disposal and recycling.
This product must not be disposed of with domestic waste or using
municipal waste disposal services. In the event of resale, the seller must
inform the buyer of the need to dispose of the product appropriately.

Table of Contents
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IMPORTANT NOTE........................................................................................ i
1. Installation.............................................................................................. 3
1.1 Safety Regulations ............................................................................ 3
General notes ................................................................................. 5
Backup copies................................................................................. 6
1.2 System Requirements........................................................................ 6
Contents of delivery........................................................................ 6
Setting up the camera system .................................................................. 7
Operating the camera on a PC........................................................ 8
Operating the camera on a PC using the FireWire PCI bus
interface card ............................................................................ 8
Installing the PCI bus interface card on the PC........................ 8
Connecting the camera to the PC......................................... 10
Operating the camera on a PC with onboard FireWire interface11
Connecting the camera to the PC......................................... 11
Operating the camera on a notebook ........................................... 12
Operating the camera on a notebook using the FireWire PCcard
(CardBus) interface card .......................................................... 12
Installing the PCcard (CardBus) interface card on the notebook
............................................................................................12
Connecting the camera to the notebook ..............................14
Operating the camera on a notebook with onboard FireWire
interfaces................................................................................. 15
Connecting the camera to the notebook .............................. 15
Mounting the camera onto the microscope ............................. 17
Function indicator.................................................................... 17
Expansion socket ..................................................................... 18
Second FireWire socket............................................................ 18
Checking the equipment has been set up correctly....................... 18
Installing the driver software .................................................................. 19
General......................................................................................... 19
Installing the camera driver with the hardware wizard .................. 20
Installing the camera driver using the setup program.................... 23
How to correct the driver entry in the device manager............. 27
Resetting the AxioCam MR firmware....................................... 29
AxioCam MR Installation + Reference i

Table of Contents
1.3 Notes on Maintenance.................................................................... 30
Camera electronic......................................................................... 30
Optical system .............................................................................. 30
Cleaning the infrared barrier filter................................................. 31
Important note on the use of C-mount objectives......................... 31
2. Image Acquisition ................................................................................ 32
2.1 General........................................................................................... 32
2.2 Quick Guide to the First Image with AxioVision............................... 33
The workflow Standard................................................................. 34
Step by step to the first image ...................................................... 35
2.3 Adjust Property Page....................................................................... 42
Exposure....................................................................................... 43
Automatic exposure................................................................. 44
White balance............................................................................... 45
Color adjustment..................................................................... 46
Color saturation............................................................................ 47
Histogram..................................................................................... 47
2.4 Frame Property Page ....................................................................... 49
Camera mode............................................................................... 49
Resolution................................................................................ 50
Binning.................................................................................... 52
Size of the resulting files.......................................................... 52
Frame ........................................................................................... 53
2.5 General Property Page .................................................................... 54
Digital gain ................................................................................... 55
Image orientation ......................................................................... 55
Black reference ............................................................................. 56
Shading correction........................................................................ 57
Sharpening ................................................................................... 60
Shutter control.............................................................................. 64
Filter operations ............................................................................ 66
2.6 Practical Notes on Operation in AxioVision...................................... 67
Reset camera to a default state..................................................... 67
Speed of the live image................................................................. 68
Notes on optimum color reproduction in AxioVision ..................... 69
2.7 Information for Displaying 12 Bit AxioCam Images in AxioVision..... 71
Recommended camera settings .................................................... 73
Recommended monitor settings ................................................... 73
ii M 60-3-0014 e / printed 12.2008

Table of Contents
2.8 Background Information ................................................................. 74
Gamma setting............................................................................. 74
Linearity........................................................................................ 74
Configurable toolbars for operating the camera ........................... 74
3. Trouble Shooting ................................................................................. 75
3.1 Software......................................................................................... 75
3.2 Image Quality ................................................................................. 76
4. Technical Data ...................................................................................... 77
4.1 AxioCam MRc (color version) .......................................................... 77
4.2 AxioCam MRm (monochrome version)............................................ 79
4.3 Spectral Sensitivity .......................................................................... 81
AxioCam MR Installation + Reference iii

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The FireWire (IEEE 1394 a) interface is defined as a "hot plugging"
interconnection, i.e. peripherals (cameras, drives, etc.) may be connected or
disconnected while the computer is powered up. In connection with this "hot
plugging" feature in isolated cases damages being caused to interface
components were reported, presumably due to a discharge of static electricity
during the insertion of the cables.
To avoid this kind of damage, we urge you to take careful note of the following
points before inserting or removing the AxioCam MR’s FireWire cable:
Avoid hot plugging with devices that are powered via the FireWire bus, i.e.
do not insert or remove the camera while the computer is switched on.
Use only high-quality and undamaged FireWire cables.
If you cannot avoid hot plugging, please take the following information into
account:
Sequence for cable insertion:
When you connect the camera to the PC, insert the cable into the PC first,
and only then into the camera head.
When you disconnect the FireWire camera from the PC, remove the cable
from the camera head first, and only then from the PC.
In this way the cable provides protection in the event that static electricity is
discharged.
Additional interface card: As an added precaution, we also recommend that
you establish the FireWire connection via FireWire interface PCI cards rather than
the onboard FireWire interfaces. Alternatively, a FireWire hub can be connected
between the camera and PC.
Carl Zeiss is unable to accept any liability for damage that results from
failure to comply with these safety recommendations.

Installation
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The AxioCam MR is a professional, high-resolution digital camera with
FireWireinterface for universal light-microscopy applications.
There are two versions available: AxioCam MRc (color camera) and AxioCam
MRm (b/w camera).
To make it easier for you to set up the camera, please follow the instructions
in these chapters step by step.
In this manual the interface between AxioCam MR and the computer is called
FireWire. Synonym the terms IEEE 1394 a and i.LINKare also used for this
interface.
Note:
In the description that follows the term AxioCam HR is used for both the
color camera and the black/white camera. A distinction is only made
where this is necessary for technical reasons.
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Please read this chapter carefully and observe the regulations in
order to ensure your safety and the intended operation of the
system. Please observe the warnings and notes printed in this
manual and on the unit.
The digital camera AxioCam MR has been manufactured and tested by Carl
Zeiss according to the regulations specified in CE und cUL and has left the
manufacturer’s premises in perfect working order.
In order to ensure that this condition is maintained and to avoid any risks
when operating the system, the user must comply with any notes and
warnings contained in this manual.
AxioCam MR Installation + Reference 3

Installation
Exemption from statutory liability for accidents
The manufacturer shall be exempt from statutory liability for accidents should
the operator fail to observe the safety regulations.
Limitation of liability
No warranty shall be assumed by Carl Zeiss during the warranty period if the
equipment is operated without observing the safety regulations. In any such
case, Carl Zeiss shall be exempt from statutory liability for accidents resulting
from such operation.
Exemption from warranty
Carl Zeiss shall be exempt from any warranty obligations should the user fail
to observe the safety regulations. Carl Zeiss only guarantees the safety,
reliability, and performance of the system if the following safety regulations
are closely observed.
¾The electrical installations of the room where the system is to be set up
must conform to IEC requirements.
Warning:
Any interruption of the PE conductor, either internally or externally, or
removal of the earthed conductor will make the system unsafe to use.
Any intentional interruption of the earthed conductor is illegal.
Attention:
To interrupt the power supply simply disconnect the data cable.
¾Setup, expansions, re-adjustments, alterations, and repairs must only be
carried out by persons who have been authorized by Carl Zeiss.
¾Use only those cables supplied by Carl Zeiss.
¾Do not allow any cables, particularly power cords, to trail across the floor,
where they can be snagged by people walking past.
4 M 60-3-0014 e / printed 12.2008