Zeiss Smartzoom 5 User manual

Quick Guide
ZEISS Smartzoom 5
Referencing Application

ZEISS Smartzoom 5
Original Manual
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany
[email protected]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany
Document Name: ZEISS Smartzoom 5 Quick Guide
Revision: 1
Language: en-US
Effective from: 11/2020
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 Content ZEISS
Content
1 Introduction .................................................................................. 4
1.1 Preparing Referencing ................................................................................ 4
2 User Interface................................................................................ 5
3 Referencing Tasks ......................................................................... 6
3.1 Stage (Angle-Stops).................................................................................... 6
3.2 Focus Position ............................................................................................ 8
3.3 Objective.................................................................................................... 10
3.4 Tilting Accelerometer ................................................................................. 11
3.5 Ov. Image Dist. Corr................................................................................... 12
3.6 Tilting Pivot Point ....................................................................................... 14
3.7 Image Alignment........................................................................................ 18
3.8 Stitching Referencing ................................................................................. 21
3.9 3D Referencing .......................................................................................... 22
4 Troubleshooting ............................................................................ 26
4.1 Angle stops not found................................................................................ 26
4.2 Overview distortion correction: Upright position......................................... 27
4.3 Stage does not move to the displayed position........................................... 28
4.4 Stitching Referencing: Referencing Object not found.................................. 28
4.5 Best Practice: Image Alignment .................................................................. 28
4.6 Wrong error message after PC upgrade...................................................... 32
Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 3

1 Introduction | 1.1 Preparing Referencing ZEISS
1 Introduction
The Smartzoom Referencing Application is used for improving the system accuracy and find-
ing the best settings for all components that are used. We recommend to perform the full refer-
encing workflow (meaning all referencing tasks are activated) if the system was newly installed.
If you update or change components we recommend to perform the full referencing workflow
again. As you have to change objectives and tilt the stand, read the Smartzoom 5 Instruction
manual, where these procedures are described in detail.
In case of mounting or dismounting the Transmittel Light Unit (TLU) repeat the referencing
tasks Ov. Image Dist. Corr. [ } 12] and Image Alignment [ } 18].
1.1 Preparing Referencing
To prepare the Smartzoom 5 system for referencing the following steps are required:
1. Switch on the microscope.
àMake sure that the stage is empty and has a clean surface.
2. Switch on the computer.
àAfter startup of the computer, wait for at least two minutes.
3. Start the Smartzoom Referencing application.
àWhile the software starts, the hardware is initialized. When the hardware was initialized
successfully you can start the referencing tasks.
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2 User Interface ZEISS
2 User Interface
1
2
3 4
Fig.1: Smartzoom Referencing Application User interface
1Status / Progress Area
Shows information on the system status, e.g. Software version or status of referencing.
2Referencing Tasks Area
Here you can activate/deactivate the referencing tasks which should be performed. By
clicking on the Start referencing button all activated referencing tasks will be per-
formed one after another.
3Preview Area
Shows the live image from the microscope camera. You will see the referencing proce-
dure of each step in here. Additionally, after starting a referencing task, you will see the
Referencing Wizard in a small dialog window in the upper right corner.
4Referencing Wizard
With the help of this wizard, you are able to control the referencing procedure of each
referencing step, e.g. Start/ Stop/ Continue buttons for controlling the referencing task.
The wizard contains instructions which will guide you through each step during a refer-
encing task. The instructions are shown in the blue box in the middle of the wizard win-
dow.
Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 5

3 Referencing Tasks | 3.1 Stage (Angle-Stops) ZEISS
3 Referencing Tasks
This chapter describes how to perform the referencing tasks available. We recommend to perform
the full referencing workflow (meaning all referencing tasks are activated) if the system was newly
installed. The entire workflow will take about 60 minutes with all objectives. When you exchange
a component (e.g. the optical engine) we recommend to perform the full referencing workflow
again.
3.1 Stage (Angle-Stops)
This referencing task will find the best settings for the microscope stage. Therefore the angle-
stops of the stage insert plate must be facing up. As you have to mount an objective, please read
the Smartzoom 5 instruction manual, where this procedure is described in detail. Note that you
should always start referencing with having mounted the objective with the highest magnification
(this depends on which objective you have bought).
1. Mount the objective with the highest magnification (e.g. 10.0x objective). Ensure that the
stage is empty and that the stand is in upright position. The angle-stops of the stage insert
plate must be facing up.
2. Click on the Start referencing button in the Referencing Tasks Area.
àThe Referencing Wizard will appear in the preview area.
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3 Referencing Tasks | 3.1 Stage (Angle-Stops) ZEISS
3. Click on the Start button within the wizard.
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3 Referencing Tasks | 3.2 Focus Position ZEISS
àNow the stage referencing will be performed automatically. You will see the stage mov-
ing to the referencing points one after another. The actual point is marked green.
After the Stage referencing (Angle-Stops) the wizard will continue with the next activated step.
3.2 Focus Position
This referencing task will find the best settings for the Z-axis / Focus Position.
1. Mount the 1.6x objective.
Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 8

3 Referencing Tasks | 3.2 Focus Position ZEISS
àEnsure that the stage is empty and that the stand is in upright position. The angle-stops
of the stage insert plate must be facing up.
2. Click on the Start button.
Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 9

3 Referencing Tasks | 3.3 Objective ZEISS
àReferencing of Z-axis is performed.
After the Z-axis referencing the wizard will continue with the next activated step.
3.3 Objective
This referencing task will find the best settings for the current used objective. We highly recom-
mend to perform referencing for each new objective that you use. Note that we will not describe
the referencing of the other objectives available as the procedure is exactly the same like de-
scribed for this objective.
Quick Guide ZEISS Smartzoom 5 | en-US | Rev.1 10