Agilent Technologies 1200 series User manual

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Agilent 1200 Series
Standard and
Preparative
Autosamplers
Reference Manual

2 Agilent 1200 Series Autosampler Reference Manual
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Manual Part Number
G1329-90010
Edition
Edition 02/06
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Agilent 1200 Series Autosampler Reference Manual 3
In This Guide…
1 Installing the Autosampler
Site requirements and installation of the autosampler
2 Optimizing Performance
Autosamplers are more and more used in HPLC to improve the
productivity in the laboratories and the consistency and
accuracy of analytical results
This chapter shows how to optimize the autosamplers to
achieve best results
3 Troubleshooting and Test Functions
The modules built-in troubleshooting and test functions
4RepairingtheAutosampler
Instructions on simple, routine repair procedures as well as
more extensive repairs requiring exchange of internal parts
5 Parts and Materials
Detailed illustrations and lists for identification of parts and
materials
6Introduction to the Autosampler
An introduction to the standard and preparative autosamplers
Theory of Operation
Theory of operation of mechanical hardware, electronics, and
instrument interfaces
8 Introduction to the Control Module
Introduction to the screens available for operation of the
Agilent 1200 Series autosamplers with the control module

4 Agilent 1200 Series Autosampler Reference Manual
9 Specifications
Performance specifications of the Agilent 1200 Series Standard
and Preparative Autosamplers

Agilent 1200 Series Autosampler Reference Manual 5
Contents
1 Installing the Autosampler 13
Site Requirements 14
Power Consideration 14
Power Cords 15
Bench Space 15
Environment 16
Unpacking the Autosampler 18
Optimizing the Stack Configuration 21
Installing the Autosampler 26
Installing the Thermostatted Autosampler 29
Flow Connections 34
Sample Trays 36
Choice of Vials and Caps 39
Transporting the Autosampler 42
2 Optimizing Performance 43
Optimization for Lowest Carry-over 44
Using the Automated Needle Wash 45
Using an Injector Program 46
General Recommendation to Lowest Carry-over 47
Fast Injection Cycle and Low Delay Volume 48
Overlapped Injection Mode 48
General Recommendations for Fast Injection Cycle Times 49
Precise Injection Volume 50

6 Agilent 1200 Series Autosampler Reference Manual
Contents
Draw and Eject Speed 50
Choice of Rotor Seal 52
3 Troubleshooting and Test Functions 53
Overview of the Sampler’s Indicators and Test Functions 54
Status Indicators 55
Power Supply Indicator 55
Instrument Status Indicator 56
Error Messages 57
Timeout 58
Shutdown 59
Remote Timeout 60
Synchronization Lost 61
Leak 62
Leak Sensor Open 63
Leak Sensor Short 64
Compensation Sensor Open 65
Compensation Sensor Short 66
Fan Failed 67
Open Cover 68
Restart Without Cover 69
Arm Movement Failed 70
Valve to Bypass Failed 71
Valve to Mainpass Failed 72
Needle Up Failed 73
Needle Down Failed 74
Missing Vial 75
Initialization Failed 76
Metering Home Failed 77
Motor Temperature 78
Initialization with Vial 79
Safety Flap Missing 80

Contents
Agilent 1200 Series Autosampler Reference Manual 7
Vial in Gripper 81
Missing Wash Vial 82
Invalid Vial Position 83
Maintenance Functions 84
User Interface 85
Change Needle 86
Change Piston 88
Park Arm (Park Gripper) 89
Change Gripper (Change Arm) 90
Tray Alignment 91
Step Commands 93
Troubleshooting 95
Troubleshooting Guide for the Sample Transport Assembly 97
Intermittent lock-ups with or without vial in the gripper fingers 98
Jittery (shaky) movement in X and or theta axes and/or when the needle goes
through the gripper arm into the via 100
Poor alignment, seen during vial pickupand vial replacement and/or when the
needle hits the gripper arm 102
4RepairingtheAutosampler105
Introduction into Repairing the Autosampler 106
Using the ESD Strap 107
Overview of Main Repair Procedures 109
Simple Repairs 110
Needle Assembly 111
Needle-Seat Assembly 114
Stator Face 117
Rotor Seal 120
Metering Seal and Plunger 124
Gripper Arm 128

8 Agilent 1200 Series Autosampler Reference Manual
Contents
Interface Board 131
Exchanging Internal Parts 132
Assembling the Main Cover 133
Top Cover and Foam 134
Installing the Top Cover and Foam 136
Illumination Assembly 138
Transport Assembly 139
Installing the Transport Assembly 140
Sampling Unit 141
Installing the Sampling Unit 144
Injection-Valve Assembly 147
Metering-Drive Motor and Belt 150
Needle-Drive Motor and Belt 152
Fan 155
ASM Board 157
Changing the Product Number or Serial Number 160
Using the Agilent ChemStation 160
Using the Instant Pilot G4208A 161
Using the Control Module G1323B 162
SUD Board 167
Power Supply 169
Leak Sensor 172
5 Parts and Materials 175
Main Assemblies 176
Sampling Unit Assembly 178
Analytical-Head Assembly 181
Injection-Valve Assembly 184
Sheet Metal Kit 186
Cover Parts 187

Contents
Agilent 1200 Series Autosampler Reference Manual 9
Foam Parts 188
Power and Status Light Pipes 189
Leak System Parts 190
Vial Trays 191
Standard Autosampler Accessory Kit G1329-68725 192
Preparative Autosampler Accessory Kit G2260-68705 193
Maintenance Kit G1313-68709 194
Multi-Draw Kit G1313-68711 195
900 µl Injection Upgrade Kit G1363A for G1329A 196
External Tray G1313-60004 197
6 Identifying Cables 199
Cable Overview 200
Analog Cables 202
Remote Cables 205
BCD Cables 210
Auxiliary Cable 212
CAN Cable 213
External Contact Cable 214
RS-232 Cable Kit 215
LAN Cables 216
7 Introduction to the Autosampler 217
Introduction to the Autosampler 218
Sampling Sequence 220
Injection Sequence 221
Sampling Unit 223
Needle-Drive 224
Analytical head / preparative head 224

10 Agilent 1200 Series Autosampler Reference Manual
Contents
Injection-Valve 225
Transport Assembly 226
Early Maintenance Feedback (EMF) 228
EMF Counters 228
Using the EMF Counters 229
Electrical Connections 230
8 Theory of Operation 233
Autosampler Control and Electronics 234
Position and Movement Sensors 235
Autosampler Main Board (ASM) 236
Firmware Description 241
Firmware Updates 242
Optional Interface Boards 243
BCD Board 243
LAN Communication Interface Board 245
Interfaces 247
Analog Signal Output 248
GPIB Interface 248
CAN Interface 248
Remote Interface 248
RS-232C 250
Setting the 8-bit Configuration Switch 252
GPIB Default Addresses 253
Communication Settings for RS-232C Communication 254
Forced Cold Start Settings 255
Stay-Resident Settings 256
The Main Power Supply Assembly 257