Agilent Technologies 1200 series User manual

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Agilent 1200 Series
Standard and
Preparative
Autosamplers
Reference Manual

2 Agilent 1200 Series Autosampler Reference Manual
Notices
© Agilent Technologies, Inc. 2006
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Manual Part Number
G1329-90010
Edition
Edition 02/06
Printed in Germany
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Agilent 1200 Series Autosampler Reference Manual 3
In This Guide…
1 Installing the Autosampler
Site requirements and installation of the autosampler
2 Optimizing Performance
Autosamplers are more and more used in HPLC to improve the
productivity in the laboratories and the consistency and
accuracy of analytical results
This chapter shows how to optimize the autosamplers to
achieve best results
3 Troubleshooting and Test Functions
The modules built-in troubleshooting and test functions
4RepairingtheAutosampler
Instructions on simple, routine repair procedures as well as
more extensive repairs requiring exchange of internal parts
5 Parts and Materials
Detailed illustrations and lists for identification of parts and
materials
6Introduction to the Autosampler
An introduction to the standard and preparative autosamplers
Theory of Operation
Theory of operation of mechanical hardware, electronics, and
instrument interfaces
8 Introduction to the Control Module
Introduction to the screens available for operation of the
Agilent 1200 Series autosamplers with the control module

4 Agilent 1200 Series Autosampler Reference Manual
9 Specifications
Performance specifications of the Agilent 1200 Series Standard
and Preparative Autosamplers

Agilent 1200 Series Autosampler Reference Manual 5
Contents
1 Installing the Autosampler 13
Site Requirements 14
Power Consideration 14
Power Cords 15
Bench Space 15
Environment 16
Unpacking the Autosampler 18
Optimizing the Stack Configuration 21
Installing the Autosampler 26
Installing the Thermostatted Autosampler 29
Flow Connections 34
Sample Trays 36
Choice of Vials and Caps 39
Transporting the Autosampler 42
2 Optimizing Performance 43
Optimization for Lowest Carry-over 44
Using the Automated Needle Wash 45
Using an Injector Program 46
General Recommendation to Lowest Carry-over 47
Fast Injection Cycle and Low Delay Volume 48
Overlapped Injection Mode 48
General Recommendations for Fast Injection Cycle Times 49
Precise Injection Volume 50

6 Agilent 1200 Series Autosampler Reference Manual
Contents
Draw and Eject Speed 50
Choice of Rotor Seal 52
3 Troubleshooting and Test Functions 53
Overview of the Sampler’s Indicators and Test Functions 54
Status Indicators 55
Power Supply Indicator 55
Instrument Status Indicator 56
Error Messages 57
Timeout 58
Shutdown 59
Remote Timeout 60
Synchronization Lost 61
Leak 62
Leak Sensor Open 63
Leak Sensor Short 64
Compensation Sensor Open 65
Compensation Sensor Short 66
Fan Failed 67
Open Cover 68
Restart Without Cover 69
Arm Movement Failed 70
Valve to Bypass Failed 71
Valve to Mainpass Failed 72
Needle Up Failed 73
Needle Down Failed 74
Missing Vial 75
Initialization Failed 76
Metering Home Failed 77
Motor Temperature 78
Initialization with Vial 79
Safety Flap Missing 80

Contents
Agilent 1200 Series Autosampler Reference Manual 7
Vial in Gripper 81
Missing Wash Vial 82
Invalid Vial Position 83
Maintenance Functions 84
User Interface 85
Change Needle 86
Change Piston 88
Park Arm (Park Gripper) 89
Change Gripper (Change Arm) 90
Tray Alignment 91
Step Commands 93
Troubleshooting 95
Troubleshooting Guide for the Sample Transport Assembly 97
Intermittent lock-ups with or without vial in the gripper fingers 98
Jittery (shaky) movement in X and or theta axes and/or when the needle goes
through the gripper arm into the via 100
Poor alignment, seen during vial pickupand vial replacement and/or when the
needle hits the gripper arm 102
4RepairingtheAutosampler105
Introduction into Repairing the Autosampler 106
Using the ESD Strap 107
Overview of Main Repair Procedures 109
Simple Repairs 110
Needle Assembly 111
Needle-Seat Assembly 114
Stator Face 117
Rotor Seal 120
Metering Seal and Plunger 124
Gripper Arm 128

8 Agilent 1200 Series Autosampler Reference Manual
Contents
Interface Board 131
Exchanging Internal Parts 132
Assembling the Main Cover 133
Top Cover and Foam 134
Installing the Top Cover and Foam 136
Illumination Assembly 138
Transport Assembly 139
Installing the Transport Assembly 140
Sampling Unit 141
Installing the Sampling Unit 144
Injection-Valve Assembly 147
Metering-Drive Motor and Belt 150
Needle-Drive Motor and Belt 152
Fan 155
ASM Board 157
Changing the Product Number or Serial Number 160
Using the Agilent ChemStation 160
Using the Instant Pilot G4208A 161
Using the Control Module G1323B 162
SUD Board 167
Power Supply 169
Leak Sensor 172
5 Parts and Materials 175
Main Assemblies 176
Sampling Unit Assembly 178
Analytical-Head Assembly 181
Injection-Valve Assembly 184
Sheet Metal Kit 186
Cover Parts 187

Contents
Agilent 1200 Series Autosampler Reference Manual 9
Foam Parts 188
Power and Status Light Pipes 189
Leak System Parts 190
Vial Trays 191
Standard Autosampler Accessory Kit G1329-68725 192
Preparative Autosampler Accessory Kit G2260-68705 193
Maintenance Kit G1313-68709 194
Multi-Draw Kit G1313-68711 195
900 µl Injection Upgrade Kit G1363A for G1329A 196
External Tray G1313-60004 197
6 Identifying Cables 199
Cable Overview 200
Analog Cables 202
Remote Cables 205
BCD Cables 210
Auxiliary Cable 212
CAN Cable 213
External Contact Cable 214
RS-232 Cable Kit 215
LAN Cables 216
7 Introduction to the Autosampler 217
Introduction to the Autosampler 218
Sampling Sequence 220
Injection Sequence 221
Sampling Unit 223
Needle-Drive 224
Analytical head / preparative head 224

10 Agilent 1200 Series Autosampler Reference Manual
Contents
Injection-Valve 225
Transport Assembly 226
Early Maintenance Feedback (EMF) 228
EMF Counters 228
Using the EMF Counters 229
Electrical Connections 230
8 Theory of Operation 233
Autosampler Control and Electronics 234
Position and Movement Sensors 235
Autosampler Main Board (ASM) 236
Firmware Description 241
Firmware Updates 242
Optional Interface Boards 243
BCD Board 243
LAN Communication Interface Board 245
Interfaces 247
Analog Signal Output 248
GPIB Interface 248
CAN Interface 248
Remote Interface 248
RS-232C 250
Setting the 8-bit Configuration Switch 252
GPIB Default Addresses 253
Communication Settings for RS-232C Communication 254
Forced Cold Start Settings 255
Stay-Resident Settings 256
The Main Power Supply Assembly 257

Contents
Agilent 1200 Series Autosampler Reference Manual 11
9 Specifications 259
Performance Specifications 260
A Safety Information 263
General Safety Information 264
Lithium Batteries Information 267
Radio Interference 268
Sound Emission 269
Solvent Information 270
Agilent Technologies on Internet 272
Index 273

12 Agilent 1200 Series Autosampler Reference Manual
Contents

13
Agilent 1200 Series Standard and Preparative Autosamplers
Reference Manual
Agilent Technologies
1
Installing the Autosampler
Site Requirements 14
Unpacking the Autosampler 18
Optimizing the Stack Configuration 21
Installing the Autosampler 26
Installing the Thermostatted Autosampler 29
Flow Connections 34
Sample Trays 36
Choice of Vials and Caps 39
Transporting the Autosampler 42

14 Agilent 1200 Series Autosampler Reference Manual
1 Installing the Autosampler
Site Requirements
A suitable site environment is important to ensure optimal performance of the
autosampler.
Power Consideration
The autosampler power supply has wide-ranging capability. Consequently
there is no voltage selector in the rear of the autosampler. There are also no
externally accessible fuses, because automatic electronic fuses are
implemented in the power supply.
The thermostatted autosampler comprises two modules, the standard or
preparative autosampler and the thermostat (G1330B). Both modules have a
separate power supply and a power plug for the line connections. The two
modules are connected by a control cable and both are turned on by the
autosampler module.
WARNING To disconnect the autosampler from line power, unplug the power cord. The power
supply still uses some power, even if the power switch on the front panel is turned
off.
WARNING To disconnect the thermostatted autosampler from line power, unplug the power
cord from the autosampler and the ALS thermostat. The power supplies still use
some power, even if the power switch on the front panel is turned off. Please make
sure that it is always possible to access the power plug.
WARNING Shock hazard or damage of your instrumentation can result if the devices are
connected to a line voltage higher than specified.

Agilent 1200 Series Autosampler Reference Manual 15
Installing the Autosampler 1
Power Cords
Your autosampler is delivered with a power cord which matches the wall
socket of your particular country or region. The plug on the power cord which
connects to the rear of the instrument is identical for all types of power cord.
Bench Space
The autosampler dimensions and weight (see Table 1 on page 16) allow the
instrument to be placed on almost any laboratory bench. The instrument
requires an additional 2.5 cm (1.0 inch) of space on either side, and
approximately 8 cm (3.1 inches) at the rear for the circulation of air, and room
for electrical connections. Ensure the autosampler is installed in a horizontal
position.
The thermostatted autosampler dimensions and weight allow the instrument
to be placed on almost any laboratory bench. The instrument requires an
additional 25 cm (10 inches) of space on either side for the circulation of air,
and approximately 8 cm (3.1 inches) at the rear for electrical connections.
Ensure the autosampler is installed in a level position.
If a complete Agilent 1200 Series system is to be installed on the bench, make
sure that the bench is designed to carry the weight of all the modules. For a
complete system including the thermostatted autosampler it is recommended
to position the modules in two stacks, see “Optimizing the Stack
Configuration” on page 21. Make sure that in this configuration there is 25 cm
(10 inches) space on either side of the thermostatted autosampler for the
circulation of air.
WARNING Never operate your instrumentation from a power outlet that has no ground
connection. Never use a power cord other than the power cord designed for your
region.
WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.

16 Agilent 1200 Series Autosampler Reference Manual
1 Installing the Autosampler
Environment
Your autosampler will work within specifications at ambient temperatures
and relative humidity as described in Table 1.
CAUTION Do not store, ship or use your autosampler under conditions where temperature
fluctuations may cause condensation within the autosampler. Condensationwill
damagethesystemelectronics.Ifyourautosamplerwasshippedincoldweather,leave
it in its box, and allow it to warm up slowly to roomtemperature to avoidcondensation.
Table 1 Physical Specifications - Autosampler (G1329A / G2260A)
Type Specification Comments
Weight 14.2 kg (31.3 lbs)
Dimensions
(height × width × depth) 200 × 345 × 435 mm (8 × 13.5 × 17 inches)
Line voltage 100 – 120 or 220– 240 VAC, ± 10 % Wide-ranging capability
Line frequency 50 or 60 Hz, ±5%
Power consumption (apparent power)
Power consumption (active power) 300 VA 200 W Maximum Maximum
Ambient operating temperature 4 – 55 °C (41 – 131 °F) see on page 17
Ambient non-operating temperature -40 to 70 °C (-4 to 158 °F)
Humidity < 95 %, at 25 – 40 °C (77 – 104 °F) Non-condensing
Operating Altitude Up to 2000 m (6500 ft)
Non-operating altitude Up to 4600 m (14950 ft) For storing the
autosampler
Safety standards: IEC, CSA, UL Installation Category II, Pollution Degree 2
For inddor use only
WARNING Using the autosampler at environmental temperatures higher than 50 °C (122 °F)
may cause the rear panel to become hot.

Agilent 1200 Series Autosampler Reference Manual 17
Installing the Autosampler 1
Unpacking the Autosampler
Damaged Packaging
Upon receipt of your autosampler, inspect the shipping containers for any
signs of damage. If the containers or cushioning material are damaged, save
them until the contents have been checked for completeness and the
autosampler has been mechanically and electrically checked. If the shipping
container or cushioning material is damaged, notify the carrier and save the
shipping material for the carriers inspection.
Delivery Checklist
Ensure all parts and materials have been delivered with the autosampler. The
instrument box contains the instrument and an Accessory kit. A separate box
contains the reference manual and the power cable.
In Table 2 on page 19 and Table 3 on page 20 are listed the content of each
accessory kit.
Please report missing or damaged parts to your local Agilent Technologies
sales and service office.
CAUTION If you need to ship the autosampler at a later date, always park thetransport assembly
before shipment (see “Transporting the Autosampler” on page 42).
CAUTION If there are signs of damage to the autosampler, please do not attempt to install the
autosampler.

18 Agilent 1200 Series Autosampler Reference Manual
1 Installing the Autosampler
Table 2 G1329A - Standard Autosampler Accessory Kit Contents
G1329-68725
Description Part Number
Tubing assembly 5063-6527
Filter promo kit 5064-8240
CAN cable, 1 m long 5181-1519
Screw capvials, clear 100/pk 5182-0714
Blue screw caps 100/pk 5182-0717
Label halftray 5989-3890
Vial instruction sheet no PN
Wrenches 1/4 - 5/16 inch 8710-0510
Rheotool socket wrench 1/4 inch 8710-2391
Hex key 4 mm, 15cm long, T-handle 8710-2392
Hex key 9/64 inch, 15 cm long, T- handle 8710-2394
Hex key 2.5 mm, 15 cm long, straight handle 8710-2412
ESD wrist strap 9300-1408
Finger caps (x3)*
* Reorder gives pack of 15
5063-6506
Front door cooled autosampler G1329-40301
Air channel adapter G1329-43200
Cover insulation no PN
Capillary 0.17 mm, 900 mm G1329-87300
Capillary heat exchanger 01090-87306
Note for Agilent 1200 Series Autosampler door upgrade no PN

Agilent 1200 Series Autosampler Reference Manual 19
Installing the Autosampler 1
Table 3 G2260A - Preparative Autosampler Accessory Kit Contents G2260-68705
Description Part Number
Tubing assembly 5063-6527
Filter promo kit 5064-8240
CAN cable, 1 m long 5181-1519
Screw cap vials, clear 100/pk 5182-0714
Blue screw caps 100/pk 5182-0717
Label halftray 5989-3890
Wrenches 1/4 - 5/16 inch 8710-0510
Rheotool socket wrench 1/4 inch 8710-2391
Hex key 4 mm, 15 cm long, T-handle 8710-2392
Hex key 9/64 inch, 15 cm long, T- handle 8710-2394
Hex key 2.5 mm, 15 cm long, straight handle 8710-2412
ESD wrist strap 9300-1408
Finger caps x3 (reorder gives pack of 15) 5063-6506
Front door cooled autosampler G1329-40301
Air channel adapter G1329-43200
Tray for 15 x 6 ml vials (x2) G1313-44513
Union, loop extension 5022-2133
Seat extension capillary (500 µl) G1313-87307
Seat extension capillary (1500 µl) G1313-87308
Sampler - Column capillary G2260-87300

20 Agilent 1200 Series Autosampler Reference Manual
1 Installing the Autosampler
Optimizing the Stack Configuration
If your autosampler is part of a system, you can ensure optimum performance
by installing the autosampler in the stack in the position shown in Figure 1 on
page 22 and Figure 2 on page 23. Figure 3 on page 24 and Figure 4 on page 25
show the configuration recommended for a thermostatted autosampler. These
configurations optimize the system flow path, ensuring minimum delay
volume.