Agilent Technologies 1220 Infinity II LC User manual

Agilent Technologies
Agilent InfinityLab LC Series
1220 Infinity II LC System
User Manual

1220 Infinity II LC System User Manual
Notices
© Agilent Technologies, Inc. 2016
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Manual Part Number
G4280-90020 Rev. B
Edition
09/2016
Printed in Germany
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76337 Waldbronn
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1220 Infinity II LC System User Manual 3
In This Guide...
In This Guide...
This manual covers the Agilent 1220 Infinity II LC System configurations:
•G4286B
•G4288B/C
•G4290B/C
•G4294B
1Introduction
This chapter provides an overview of the Agilent 1220 Infinity II LC available
configurations.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installation
This chapter provides an overview on shipment content and installation.
4 LAN Configuration
This chapter provides information on connecting the instrument to the Agilent
ChemStation PC.
5 Solvent Delivery System Description
This chapter provides an overview on the operational principles of the solvent
delivery system (pump and optional degasser).
6InjectionSystemDescription
This chapter provides an overview of the operational principles of the
injection systems: manual injector and autosampler.

4 1220 Infinity II LC System User Manual
In This Guide...
7 Column Oven Description
This chapter provides an overview of the operational principles of the column
oven.
8 Detector Description
This chapter provides an overview of the operational principles of the
detector.
9 Test Functions and Calibration
This chapter describes the tests, calibrations and tools that are available with
the Instrument Utilities software or the Lab Advisor (Service and Diagnostics
Section).
10 Error Information
This chapter provides information on the error messages that might be
displayed, and gives the possible causes and suggestions on their solutions.
11 Maintenance and Repair
This chapter provides general information on maintenance and repair of the
instrument.
12 Parts for Maintenance and Repair
This chapter provides information on parts for maintenance and repair.
13 Upgrading the 1220 Infinity II LC
This chapter provides information for upgrading the LC system.
14 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
15 Appendix
This chapter provides addition information on safety, legal and web.

1220 Infinity II LC System User Manual 5
Contents
Contents
1Introduction 9
Agilent 1220 Infinity II LC Configurations 10
Agilent 1220 Infinity II LC VL Configurations 11
Early Maintenance Feedback 12
2 Site Requirements and Specifications 15
Site Requirements 16
Physical Specifications 19
Performance Specifications 20
3 Installation 29
Unpacking Your System 30
Installing the Hardware 35
Connecting and Configuring the Instrument to the Chromatographic Data
System 44
Connecting the Agilent 1220 Infinity II LC to the PC 45
The Instrument Utility / Lab Advisor Software 47
Configuration of the Instrument After an Upgrade Installation 48
Priming the System and Performing the ‘Installation Check’ 49
Performing a ‘Checkout Run’ 50
Remove the Restriction Capillary 51
Install a Column 53
Remove a Column 55
Install the Restriction Capillary 57
4 LAN Configuration 59
To do firs t 60
TCP/IP parameter configuration 62
Configuration Switches 63
Initialization mode selection 64
Dynamic Host Configuration Protocol (DHCP) 66
Link configuration selection 69

6 1220 Infinity II LC System User Manual
Contents
Automatic Configuration with BootP 70
Storing the settings permanently with Bootp 79
Manual Configuration 80
5 Solvent Delivery System Description 85
Overview 86
Degasser 87
Principles of Operation 88
Compressibility Compensation 92
Variable Stroke Volume 95
Using the Pump 96
6 Injection System Description 99
Manual Injector 100
Autosampler 103
7 Column Oven Description 115
Column Oven 116
8 Detector Description 117
Detector Types 118
Agilent 1220 Infinity II LC Variable Wavelength Detector (VWD) 119
Agilent 1220 Infinity II LC Diode Array Detector (DAD) 120
Match the Flow Cell to the Column 136
9 Test Functions and Calibration 141
1220 Infinity II LC System 143
Solvent Delivery System 145
Autosampler 154
Column Oven 160
Variable Wavelength Detector (VWD) 162
Diode Array Detector (DAD) 171

1220 Infinity II LC System User Manual 7
Contents
10 Error Information 193
What are Error Messages? 196
General Error Messages 197
Pump Error Messages 203
Autosampler Error Messages 216
General Detector Error Messages 226
VWD Detector Error Messages 229
DAD Detector Error Messages 235
11 Maintenance and Repair 241
PM Scope of Work and Checklist 243
Cautions and Warnings 244
Solvent Delivery System 246
Manual Injector 267
Autosampler 271
Variable Wavelength Detector (VWD) 290
Diode Array Detector (DAD) 302
Algae Growth in HPLC Systems 323
Replacing the Module’s Firmware 325
12 Parts for Maintenance and Repair 327
1220 Infinity II LC System 328
Solvent Delivery System 331
Injection System 340
Column Oven 347
Detector 348
13 Upgrading the 1220 Infinity II LC 355
Oven Upgrade 356
Install the (optional) External Tray 359
Install the Active Inlet Valve 363

8 1220 Infinity II LC System User Manual
Contents
14 Identifying Cables 367
Cable Overview 368
Analog Cables 370
Remote Cables 372
BCD Cables 375
CAN/LAN Cables 377
Agilent 1200 module to PC 378
15 Appendix 379
General Safety Information 380
Solvent Information 383
Radio Interference 385
UV Radiation 386
Sound Emission 387
Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) 388
Declaration of Conformity for HOX2 Filter 389
Agilent Technologies on Internet 390

9
1220 Infinity II LC System User Manual
Agilent Technologies
1
Introduction
Agilent 1220 Infinity II LC Configurations 10
Agilent 1220 Infinity II LC VL Configurations 11
Early Maintenance Feedback 12
EMF counters for the pump 12
EMF counters for the autosampler 12
EMF counters for the variable wavelength detector 13
EMF counters for the diode array detector 14
This chapter provides an overview of the Agilent 1220 Infinity II LC available
configurations.

10 1220 Infinity II LC System User Manual
1 Introduction
Agilent 1220 Infinity II LC Configurations
Agilent 1220 Infinity II LC Configurations
Available configurations of Agilent 1220 Infinity II LC
The Agilent 1220 Infinity II LC is available in four different configurations.
Possible components include isocratic pump, dual-channel gradient pump
(with degasser), manual injector, autosampler, column oven and detector.
Each configuration comes with at least one pump, one injection system and
one detector and includes Agilent Instrument Utilities Software.
A Solvent Selection Valve (SSV) Upgrade Kit (G4280-68708) is available.
A 1220 Infinity II AIV Upgrade Kit (G5699B) is available to handle
applications with high buffer concentration.
Variable
wavelength
detector
Variable
wavelength
detector
Variable
wavelength
detector
Isocratic pump Gradient pump Gradient pump Gradient pump
Manual injector Manual injector Autosampler Autosampler
Column oven
Diode
array
detector
Column oven
G4286B G4288B G4290B G4294B