Agilent Technologies 1220 Infinity II LC User manual

Agilent Technologies
Agilent InfinityLab LC Series
1220 Infinity II LC System
User Manual

1220 Infinity II LC System User Manual
Notices
© Agilent Technologies, Inc. 2016
No part of this manual may be reproduced
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tion into a foreign language) without prior
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lent Technologies, Inc. as governed by
United States and international copyright
laws.
Manual Part Number
G4280-90020 Rev. B
Edition
09/2016
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
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A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
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WARNING notice until the indi-
cated conditions are fully
understood and met.

1220 Infinity II LC System User Manual 3
In This Guide...
In This Guide...
This manual covers the Agilent 1220 Infinity II LC System configurations:
•G4286B
•G4288B/C
•G4290B/C
•G4294B
1Introduction
This chapter provides an overview of the Agilent 1220 Infinity II LC available
configurations.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installation
This chapter provides an overview on shipment content and installation.
4 LAN Configuration
This chapter provides information on connecting the instrument to the Agilent
ChemStation PC.
5 Solvent Delivery System Description
This chapter provides an overview on the operational principles of the solvent
delivery system (pump and optional degasser).
6InjectionSystemDescription
This chapter provides an overview of the operational principles of the
injection systems: manual injector and autosampler.

4 1220 Infinity II LC System User Manual
In This Guide...
7 Column Oven Description
This chapter provides an overview of the operational principles of the column
oven.
8 Detector Description
This chapter provides an overview of the operational principles of the
detector.
9 Test Functions and Calibration
This chapter describes the tests, calibrations and tools that are available with
the Instrument Utilities software or the Lab Advisor (Service and Diagnostics
Section).
10 Error Information
This chapter provides information on the error messages that might be
displayed, and gives the possible causes and suggestions on their solutions.
11 Maintenance and Repair
This chapter provides general information on maintenance and repair of the
instrument.
12 Parts for Maintenance and Repair
This chapter provides information on parts for maintenance and repair.
13 Upgrading the 1220 Infinity II LC
This chapter provides information for upgrading the LC system.
14 Identifying Cables
This chapter provides information on cables used with the Agilent 1200
Infinity Series modules.
15 Appendix
This chapter provides addition information on safety, legal and web.

1220 Infinity II LC System User Manual 5
Contents
Contents
1Introduction 9
Agilent 1220 Infinity II LC Configurations 10
Agilent 1220 Infinity II LC VL Configurations 11
Early Maintenance Feedback 12
2 Site Requirements and Specifications 15
Site Requirements 16
Physical Specifications 19
Performance Specifications 20
3 Installation 29
Unpacking Your System 30
Installing the Hardware 35
Connecting and Configuring the Instrument to the Chromatographic Data
System 44
Connecting the Agilent 1220 Infinity II LC to the PC 45
The Instrument Utility / Lab Advisor Software 47
Configuration of the Instrument After an Upgrade Installation 48
Priming the System and Performing the ‘Installation Check’ 49
Performing a ‘Checkout Run’ 50
Remove the Restriction Capillary 51
Install a Column 53
Remove a Column 55
Install the Restriction Capillary 57
4 LAN Configuration 59
To do firs t 60
TCP/IP parameter configuration 62
Configuration Switches 63
Initialization mode selection 64
Dynamic Host Configuration Protocol (DHCP) 66
Link configuration selection 69

6 1220 Infinity II LC System User Manual
Contents
Automatic Configuration with BootP 70
Storing the settings permanently with Bootp 79
Manual Configuration 80
5 Solvent Delivery System Description 85
Overview 86
Degasser 87
Principles of Operation 88
Compressibility Compensation 92
Variable Stroke Volume 95
Using the Pump 96
6 Injection System Description 99
Manual Injector 100
Autosampler 103
7 Column Oven Description 115
Column Oven 116
8 Detector Description 117
Detector Types 118
Agilent 1220 Infinity II LC Variable Wavelength Detector (VWD) 119
Agilent 1220 Infinity II LC Diode Array Detector (DAD) 120
Match the Flow Cell to the Column 136
9 Test Functions and Calibration 141
1220 Infinity II LC System 143
Solvent Delivery System 145
Autosampler 154
Column Oven 160
Variable Wavelength Detector (VWD) 162
Diode Array Detector (DAD) 171

1220 Infinity II LC System User Manual 7
Contents
10 Error Information 193
What are Error Messages? 196
General Error Messages 197
Pump Error Messages 203
Autosampler Error Messages 216
General Detector Error Messages 226
VWD Detector Error Messages 229
DAD Detector Error Messages 235
11 Maintenance and Repair 241
PM Scope of Work and Checklist 243
Cautions and Warnings 244
Solvent Delivery System 246
Manual Injector 267
Autosampler 271
Variable Wavelength Detector (VWD) 290
Diode Array Detector (DAD) 302
Algae Growth in HPLC Systems 323
Replacing the Module’s Firmware 325
12 Parts for Maintenance and Repair 327
1220 Infinity II LC System 328
Solvent Delivery System 331
Injection System 340
Column Oven 347
Detector 348
13 Upgrading the 1220 Infinity II LC 355
Oven Upgrade 356
Install the (optional) External Tray 359
Install the Active Inlet Valve 363

8 1220 Infinity II LC System User Manual
Contents
14 Identifying Cables 367
Cable Overview 368
Analog Cables 370
Remote Cables 372
BCD Cables 375
CAN/LAN Cables 377
Agilent 1200 module to PC 378
15 Appendix 379
General Safety Information 380
Solvent Information 383
Radio Interference 385
UV Radiation 386
Sound Emission 387
Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) 388
Declaration of Conformity for HOX2 Filter 389
Agilent Technologies on Internet 390

9
1220 Infinity II LC System User Manual
Agilent Technologies
1
Introduction
Agilent 1220 Infinity II LC Configurations 10
Agilent 1220 Infinity II LC VL Configurations 11
Early Maintenance Feedback 12
EMF counters for the pump 12
EMF counters for the autosampler 12
EMF counters for the variable wavelength detector 13
EMF counters for the diode array detector 14
This chapter provides an overview of the Agilent 1220 Infinity II LC available
configurations.

10 1220 Infinity II LC System User Manual
1 Introduction
Agilent 1220 Infinity II LC Configurations
Agilent 1220 Infinity II LC Configurations
Available configurations of Agilent 1220 Infinity II LC
The Agilent 1220 Infinity II LC is available in four different configurations.
Possible components include isocratic pump, dual-channel gradient pump
(with degasser), manual injector, autosampler, column oven and detector.
Each configuration comes with at least one pump, one injection system and
one detector and includes Agilent Instrument Utilities Software.
A Solvent Selection Valve (SSV) Upgrade Kit (G4280-68708) is available.
A 1220 Infinity II AIV Upgrade Kit (G5699B) is available to handle
applications with high buffer concentration.
Variable
wavelength
detector
Variable
wavelength
detector
Variable
wavelength
detector
Isocratic pump Gradient pump Gradient pump Gradient pump
Manual injector Manual injector Autosampler Autosampler
Column oven
Diode
array
detector
Column oven
G4286B G4288B G4290B G4294B

1220 Infinity II LC System User Manual 11
Introduction
1
Agilent 1220 Infinity II LC VL Configurations
Agilent 1220 Infinity II LC VL Configurations
Available configurations of Agilent 1220 Infinity II LC VL
The Agilent 1220 Infinity II LC VL is available in two different configurations.
Possible components include isocratic pump, dual-channel gradient pump
(with degasser), manual injector, autosampler, column oven and detector.
Each configuration comes with at least one pump, one injection system and
one detector and includes Agilent Instrument Utilities Software.
A Solvent Selection Valve (SSV) Upgrade Kit (G4280-68708) is available.
A 1220 Infinity II AIV Upgrade Kit (G5699B) is available to handle
applications with high buffer concentration.
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12 1220 Infinity II LC System User Manual
1 Introduction
Early Maintenance Feedback
Early Maintenance Feedback
EMF counters for the pump
The user-settable EMF limits for the EMF counters enable the early
maintenance feedback to be adapted to specific user requirements. The wear
of pump components is dependent on the analytical conditions. Therefore, the
definition of the maximum limits needs to be determined based on the specific
operating conditions of the instrument.
The Agilent 1220 Infinity II LC pump provides a series of EMF counters for the
pump head. Each counter increments with pump use, and can be assigned a
maximum limit that provides visual feedback in the user interface when the
limit is exceeded. Each counter can be reset to zero after maintenance has
been done. The pump provides the following EMF counters:
Pump liquimeter
The pump liquimeter displays the total volume of solvent pumped by the pump
head since the last reset of the counters. The pump liquimeter can be assigned
an EMF (maximum) limit. When the limit is exceeded, the EMF flag in the user
interface is displayed.
Seal wear counters
The seal wear counters display a value derived from pressure and flow (both
contribute to seal wear). The values increment with pump usage until the
counters are reset after seal maintenance. Both seal wear counters can be
assigned an EMF (maximum) limit. When the limit is exceeded, the EMF flag in
the user interface is displayed.
EMF counters for the autosampler
The user-settable EMF limits for the EMF counters enable the early
maintenance feedback to be adapted to specific user requirements. The wear
of autosampler components is dependent on the analytical conditions.

1220 Infinity II LC System User Manual 13
Introduction
1
Early Maintenance Feedback
Therefore, the definition of the maximum limits need to be determined based
on the specific operating conditions of the instrument.
The autosampler provides two EMF counters. Each counter increments with
autosampler use, and can be assigned a maximum limit which provides visual
feedback in the user interface when the limit is exceeded. Each counter can be
reset to zero after maintenance has been done. The autosampler provides the
following EMF counters:
Injection valve counter
This counter display the total number of switches of the injection valve since
the last reset of the counter.
Needle movements counter
This counter displays the total number of movements of the needle into the
seat since the last reset of the counter.
EMF counters for the variable wavelength detector
The user-settable EMF limits for the EMF counter enables the early
maintenance feedback to be adapted to specific user requirements. The useful
lamp burn time is dependent on the requirements for the analysis (high or low
sensitivity analysis, wavelength, and so on). Therefore, the definition of the
maximum limits need to be determined based on the specific operating
conditions of the instrument.
The detector module provides a EMF counter for the lamp. The counter
increments with lamp use, and can be assigned a maximum limit which
provides visual feedback in the user interface when the limit is exceeded. The
counter can be reset to zero after the lamp is exchanged. The detector provides
the following EMF counters:
Deuterium lamp on-time
This counter shows the total burn time of the deuterium lamp in hours.

14 1220 Infinity II LC System User Manual
1 Introduction
Early Maintenance Feedback
EMF counters for the diode array detector
Using the EMF Counters
The user-settable EMF limits for the EMF Counters enable the early maintenance
feedback to be adapted to specific user requirements. The useful maintenance
cycle is dependent on the requirements for use. Therefore, the definition of the
maximum limits need to be determined based on the specific operating
conditions of the instrument.
Setting the EMF Limits
The setting of the EMF limits must be optimized over one or two maintenance
cycles. Initially the default EMF limits should be set. When instrument
performance indicates maintenance is necessary, take note of the values
displayed by the EMF counters. Enter these values (or values slightly less than
the displayed values) as EMF limits, and then reset the EMF counters to zero.
The next time the EMF counters exceed the new EMF limits, the EMF flag will be
displayed, providing a reminder that maintenance needs to be scheduled.

15
1220 Infinity II LC System User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 16
Power Considerations 16
Power Cord 17
Bench Space 17
Environment 18
Physical Specifications 19
Performance Specifications 20
Specification Conditions 27
This chapter provides information on environmental requirements, physical
and performance specifications.

16 1220 Infinity II LC System User Manual
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The Agilent 1220 Infinity II LC power supply has wide-ranging capabilities.
Consequently, there is no voltage selector at the instrument.
WARNING
Instrument is partially energized when switched off
The power supply still uses some power even when the power switch on the front
panel is turned OFF. Repair work at the detector can lead to personal injuries, e. g.
shock hazard, when the detector cover is opened and the instrument is connected
to power.
➔To disconnect the detector from the power line, unplug the power cord.
WARNING
Incorrect line voltage to the instrument
Shock hazard or damage to your instrumentation can result if the devices are
connected to a line voltage higher than specified.
➔Connect your instrument only to the specified line voltage.
CAUTION
In case of an emergency, it must be possible to disconnect the instrument from the
power line at any time.
Make sure that there is easy access to the power cable of the instrument so that the
instrument can quickly and easily be disconnected from the line voltage.
➔Provide sufficient space next to the power socket of the instrument to allow the
cable to be unplugged.

1220 Infinity II LC System User Manual 17
Site Requirements and Specifications
2
Site Requirements
Power Cord
Different power cords are offered as options with the system. The female ends
of all power cords are identical. The female end plugs into the power-input
socket at the rear left side of the instrument. The male end of each power cord
is different and designed to match the wall socket of a particular country or
region.
Bench Space
The dimensions and weight of the Agilent 1220 Infinity II LC allow it to be
placed on almost any desk or laboratory bench. It needs an additional 2.5 cm
(1.0 in) of space on either side and approximately 8 cm (3.1 in) at the rear for
air circulation and electric connections.
Make sure that the bench intended to carry the Agilent 1220 Infinity II LC is
designed to bear the weight of the instrument.
The Agilent 1220 Infinity II LC should be operated upright.
WARNING
Absence of ground connection or use of unspecified power cord
The absence of ground connection or the use of unspecified power cord can lead to
electric shock or short circuit.
➔Never operate your instrument from a power outlet that has no ground connection.
➔Never use a power cord other than the Agilent Technologies power cord designed
for your region.
WARNING
Use of cables not supplied by Agilent
Using cables that have not been supplied by Agilent Technologies can lead to
damage of the electronic components or personal injury.
➔Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.

18 1220 Infinity II LC System User Manual
2 Site Requirements and Specifications
Site Requirements
Environment
Your Agilent 1220 Infinity II LC will work within specifications at ambient
temperatures and relative humidity as described in the following sections.
ASTM drift tests require a temperature change below 2 °C/hour (3.6 °F/hour)
measured over one hour period. Our published drift specification is based on
these conditions. Larger ambient temperature changes will result in larger
drift.
Better drift performance depends on better control of the temperature
fluctuations. To realize the highest performance, minimize the frequency and
the amplitude of the temperature changes to below 1 °C/hour (1.8 °F/hour).
Turbulences around one minute or less can be ignored.
CAUTION
Condensation within the module
Condensation can damage the system electronics.
➔Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.

1220 Infinity II LC System User Manual 19
Site Requirements and Specifications
2
Physical Specifications
Physical Specifications
Table 1 Physical Specifications
Type Specification Comments
Weight 30 kg (66 lbs)
G4294B:
43 kg (94 lbs)
Dimensions
(height × width × depth)
640 × 370 × 420 mm
(25.2 × 14.6 × 16.5 inches)
G4294B:
640×370×485 mm
(25.2×14.6×19.1 inches)
Line voltage 100 – 240 V~, ± 10 % Wide-ranging
capability
Line frequency 50 or 60 Hz, ± 5 %
Power consumption 240 VA / 210 W / 717 BTU Maximum
Ambient operating
temperature
4–55 °C (39–131 °F)
Ambient non-operating
temperature
-40 – 70 °C (-40 – 158 °F)
Humidity < 95 % r.h. at 40 °C (104 °F) Non-condensing
Operating altitude Up to 2000 m (6562 ft)
Non-operating altitude Up to 4600 m (15092 ft) For storing the module
Safety standards: IEC, CSA,
UL
Installation category II, Pollution degree 2 For indoor use only.

20 1220 Infinity II LC System User Manual
2 Site Requirements and Specifications
Performance Specifications
Performance Specifications
Performance Specifications Agilent 1220 Infinity II LC
Table 2 Performance Specifications Agilent 1220 Infinity II LC
Type Specification
Safety features Extensive diagnostics, error detection and
display, leak detection, safe leak handling, leak
output signal for shutdown of pumping system.
Low voltages in major maintenance areas.
Control and data evaluation Agilent EZChrom Compact, Agilent
ChemStation, Agilent Instrument Utilities,
Agilent Lab Advisor
Communications Controller-area network (CAN), RS-232C, APG
Remote: ready, start, stop and shut-down
signals, LAN
GLP features Early maintenance feedback (EMF), electronic
records of maintenance and errors