Agilent Technologies 1290 Infinity II User manual

Agilent Technologies
Agilent InfinityLab LC Series
1290 Infinity II
MS Flow Modulator
User Manual

MS Flow Modulator User Manual
Notices
© Agilent Technologies, Inc. 2017
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lent Technologies, Inc. as governed by
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laws.
Manual Part Number
G7170-90000
Edition
09/2017
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
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Safety Notices
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A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
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notice until the indicated condi-
tions are fully understood and
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A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
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WARNING notice until the indi-
cated conditions are fully
understood and met.

MS Flow Modulator User Manual 3
In This Guide…
In This Guide…
This manual covers the Agilent InfinityLab LC Series 1290 Infinity II MS Flow
Modulator (G7170B)
1Introduction
This chapter gives an introduction to the module.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the installation of the valve drive and the
valve heads.
4 Using the Module
This chapter provides information on how to use the module.
5 Troubleshooting and Diagnostics
Overview about the troubleshooting and diagnostic features.
6 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
7 Maintenance
This chapter describes the maintenance of the module.
8 Parts and Materials
This chapter provides information on parts for maintenance.

4 MS Flow Modulator User Manual
In This Guide…
9 Identifying Cables
This chapter provides information on cables used with the modules.
10 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
11 Appendix
This chapter provides addition information on safety, legal and web.

MS Flow Modulator User Manual 5
Contents
Contents
1Introduction 7
1290 Infinity II MS Flow Modulator 8
Features (G7170B) 9
Leak and Waste (G7170B) 10
Operating Principle 11
2 Site Requirements and Specifications 13
Site Requirements 14
Physical Specifications 17
Performance Specifications 18
3 Installing the Module 19
Unpacking the Module 20
Installation of the MS Flow Modulator 21
4 Using the Module 31
Overview 32
Solvent Information 33
Status Indicator 40
Leak and Waste Handling 41
Configure the Flow Modulator 43
5 Troubleshooting and Diagnostics 47
User Interfaces 48
Agilent Lab Advisor Software 49
6 Error Information 51
What Are Error Messages 52
General Error Messages 53
Module Specific Error Messages 58

6 MS Flow Modulator User Manual
Contents
7 Maintenance 61
Introduction to Maintenance 62
Warnings and Cautions 63
Cleaning the Module 64
Replacing the Fuses of the Infinity Valve Drive 65
Replace Rotor and Stator 67
Replace Valve Heads 70
Replacing the Module Firmware 74
8 Parts and Materials 77
Accessory Kit for the Flow Modulator 78
Optional Parts 80
9 Identifying Cables 81
Cable Overview 82
Analog Cables 84
Remote Cables 86
CAN/LAN Cables 90
Agilent Module to PC 91
USB 92
10 Hardware Information 93
Electrical Connections 94
Interfaces 96
Config Switch Settings of the Infinity Valve Drive 102
11 Appendix 105
General Safety Information 106
Waste Electrical and Electronic Equipment (WEEE) Directive (2002-96-EC) 113
Radio Interference 114
Sound Emission 115
Agilent Technologies on Internet 116

7
MS Flow Modulator User Manual
Agilent Technologies
1
Introduction
1290 Infinity II MS Flow Modulator 8
Features (G7170B) 9
Leak and Waste (G7170B) 10
Operating Principle 11
This chapter gives an introduction to the module.

8 MS Flow Modulator User Manual
1 Introduction
1290 Infinity II MS Flow Modulator
1290 Infinity II MS Flow Modulator
Based on technology in our InfinityLab Quick Change valves, the 1290 Infinity
II MS Flow Modulator facilitates efficient flow control for mass-based fraction
collection on all InfinityLab LC Purification Solutions. The Flow Modulator is
a compact module suitable for flow rates up to 100 mL/min and supports split
ratios between 40:1 and 400000:1 with split frequencies up to 2 Hz.
Figure 1 Overview of the MS flow modulator
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MS Flow Modulator User Manual 9
Introduction
1
Features (G7170B)
Features (G7170B)
Purification efficiency
•Supports wide range of split modes from 40:1 up to 400000:1 depending on
flow rate
•Enables split frequencies of up to 2 Hz
•Handles flow rates up to 100 mL/min
Instrument efficiency
•Integrates into any InfinityLab LC Purification Solution through CAN-bus
technology
•Supported by Agilent drivers – no additional software or macros required
Laboratory efficiency
•Compact design and easy connection to any InfinityLab LC Purification
Solution saves bench space
•Compatible with all InfinityLab LC Purification Solutions, enabling upgrade
to mass-based fraction collection when needs demand
•Integrates seamlessly in OpenLAB CDS ChemStation for familiar and
reliable operation

10 MS Flow Modulator User Manual
1 Introduction
Leak and Waste (G7170B)
Leak and Waste (G7170B)
1
NOTE
This example shows the leak and waste concept of the module, if installed to a leak tray and attached to
another module.
Alternatively, the module can be installed and integrated to the leak and waste concept of 1290 Infinity II
Preparative Column Compartment.
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MS Flow Modulator User Manual 11
Introduction
1
Operating Principle
Operating Principle
The flow modulator is a compact, active splitting device configured to deliver
three different unique aliquots from a HPLC to a Single Quad Mass
Spectroscopic Detector while maintaining uninterrupted flow.
Aliquot Transfer
Principle of aliquot transfer (see also Figure 2 on page 11):
1In the Aliquot Fill position, HPLC effluent enters Port 1 where a portion of
the stream is directed into one of the MS Flow Modulator’s volume transfer
chambers.
2The LC stream exits from Port 2 and may be directed to a second detector
or a fraction collector.
3A Make-up pump connects to Port 3 and, in the Aliquot Transfer position,
the volume captured is transferred through Port 4 into the analytical
stream of the MS.
Figure 2 Schematics of an active flow splitter, showing the fill and transfer positions of
the valve

12 MS Flow Modulator User Manual
1 Introduction
Operating Principle
Calculation of the Split Ratio
•The MS Flow Modulator has 4 ports with 6 positions (2 x 3)
•The user can choose the size of aliquots, from three aliquots
•The valve will move the rotor between two of the same aliquots, backwards
and forwards
The user can change the gating frequency of the valve from 14 different
modes
The split ratio, resulting from the selected mode and the prep flow rate, is
calculated internally and displayed in the method.
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*URRYH

13
MS Flow Modulator User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 14
Physical Specifications 17
Performance Specifications 18
This chapter provides information on environmental requirements, physical
and performance specifications.

14 MS Flow Modulator User Manual
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line
voltage in the range described in Table 1 on page 17. Consequently there is no
voltage selector in the rear of the module. There are also no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than specified.
➔Connect your instrument to the specified line voltage only.
WARNING
The module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. electrical shock, when
the cover is opened and the module is connected to power.
➔Always unplug the power cable before opening the cover.
➔Do not connect the power cable to the instrument while the covers are removed.
WARNING
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the
power line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.

MS Flow Modulator User Manual 15
Site Requirements and Specifications
2
Site Requirements
Power Cords
Country-specific power cords are available for the module. The female end of
all power cords is identical. It plugs into the power-input socket at the rear.
The male end of each power cord is different and designed to match the wall
socket of a particular country or region.
Agilent makes sure that your instrument is shipped with the power cord that
is suitable for your particular country or region.
WARNING
Absence of ground connection
The absence of ground connection can lead to electric shock or short circuit.
➔Never operate your instrumentation from a power outlet that has no ground
connection.
WARNING
Unintended use of supplied power cords
Using power cords for unintended purposes can lead to personal injury or damage
of electronic equipment.
➔Never use a power cord other than the one that Agilent shipped with this
instrument.
➔Never use the power cords that Agilent Technologies supplies with this instrument
for any other equipment.
➔Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
WARNING
Power cords
Solvents may damage electrical cables.
➔Prevent electrical cables from getting in contact with solvents.
➔Exchange electrical cables after contact with solvents.

16 MS Flow Modulator User Manual
2 Site Requirements and Specifications
Site Requirements
Condensation
CAUTION
Condensation within the module
Condensation can damage the system electronics.
➔Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.

MS Flow Modulator User Manual 17
Site Requirements and Specifications
2
Physical Specifications
Physical Specifications
Table 1 Physical Specifications
Type Specification Comments
Weight 2.2 kg (4.9 lbs)
Dimensions
(height × width × depth)
95 x 95 x 300 mm
(3.7 x 3.7 x 11.8 inches)
Line voltage 100 – 240 V~, ± 10 % Wide-ranging
capability
Line frequency 50 or 60 Hz, ± 5 %
Power consumption 20 VA, 4 W
Ambient operating
temperature
4 – 40 °C (39 – 104 °F)
Ambient non-operating
temperature
-40 – 70 °C (-40 – 158 °F)
Humidity < 95 % r.h. at 40 °C (104 °F) Non-condensing
Operating altitude Up to 3000 m (9842 ft)
Non-operating altitude Up to 4600 m (15092 ft) For storing the module
Safety standards:
IEC, EN, CSA, UL
Installation category II, Pollution degree 2 For indoor use only.
ISM Classification ISM Group 1 Class B According to CISPR 11

18 MS Flow Modulator User Manual
2 Site Requirements and Specifications
Performance Specifications
Performance Specifications
Table 2 Performance Specifications 1290 Infinity II MS Flow Modulator (G7170B)
Feature Specification Comment
External Leak Sensor Yes controlled via ERI
Flow rate 4 – 200 mL/min
Maximum Pressure 69 bar (100 psi)
Valve Options 5067-4292 Quick-Change valve head
(user-exchangeable)
Valve Identification Via RFID tag
Instrument Control LC & CE Drivers A.02.17 or above
Instrument Control Framework (ICF) A.02.04 or
above
Instant Pilot (G4208A) with firmware B.02.22 or
above
InfinityLab LC Companion (G7108A)
Lab Advisor B.02.10 or above
For details about
supported software
versions refer to the
compatibility matrix of
your version of the LC and
CE Drivers
Communications CAN (2x) G7170B is a hosted
module. The LC stack
needs to contain a LAN
card for communication
and control.
Maintenance and
safety-related features
Leak handling with external leak sensor and
leak pane. Leak detection, safe leak handling,
leak output signal for shutdown of pumping
system, and low voltages in major
maintenance areas
Extensive diagnostics, error detection and
display with Agilent Lab Advisor software
GLP features Early maintenance feedback (EMF) for
continuous tracking of instrument usage with
user-settable limits and feedback messages.
Electronic records of maintenance and errors
Housing All materials recyclable

19
MS Flow Modulator User Manual
Agilent Technologies
3
Installing the Module
Unpacking the Module 20
Installation of the MS Flow Modulator 21
Assembling and Installing the Leak Tray and Leak Sensor 22
Mounting the MS Flow Modulator to an Instrument 23
Mount the MS Flow Modulator to the 1290 Infinity II Preparative
Column Compartment. 24
Install the MS Flow Modulator 25
Install the valve head and connecting capillaries 27
This chapter gives information about the installation of the valve drive and the
valve heads.

20 MS Flow Modulator User Manual
3 Installing the Module
Unpacking the Module
Unpacking the Module
Damaged Packaging
If the delivery packaging shows signs of external damage, please call your
Agilent Technologies sales and service office immediately. Inform your service
representative that the instrument may have been damaged during shipment.
Delivery Checklist
Ensure all parts and materials have been delivered with your module. The
delivery checklist is included to your shipment. For parts identification please
check the illustrated parts breakdown in the chapter Parts and Materials.
Please report any missing or damaged parts to your local Agilent Technologies
sales and service office.
Accessory Kit
The flow modulator is shipped with Accessory Kit (G9352-68100) (see
“Accessory Kit for the Flow Modulator” on page 78).
CAUTION
"Defective on arrival" problems
If there are signs of damage, please do not attempt to install the module. Inspection
by Agilent is required to evaluate if the instrument is in good condition or damaged.
➔Notify your Agilent sales and service office about the damage.
➔An Agilent service representative will inspect the instrument at your site and
initiate appropriate actions.