Agilent Technologies 1290 infinity User manual

Agilent Technologies
Agilent 1290 Infinity
Flexible Cube
User Manual

1290 Infinity Flexible Cube User Manual
Notices
© Agilent Technologies, Inc. 2010
No part of this manual may be reproduced
in any form or by any means (including elec-
tronic storage and retrieval or translation
into a foreign language) without prior agree-
ment and written consent from Agilent
Technologies, Inc. as governed by United
States and international copyright laws.
Manual Part Number
G4227-90000
Edition
06/2010
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a license
and may be used or copied only in accor-
dance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of a
U.S. Government prime contract or subcon-
tract, Software is delivered and licensed as
“Commercial computer software” as
defined in DFAR 252.227-7014 (June 1995),
or as a “commercial item” as defined in FAR
2.101(a) or as “Restricted computer soft-
ware” as defined in FAR 52.227-19 (June
1987) or any equivalent agency regulation
or contract clause. Use, duplication or dis-
closure of Software is subject to Agilent
Technologies’ standard commercial license
terms, and non-DOD Departments and
Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.
For Research Use Only

1290 Infinity Flexible Cube User Manual 3
In This Guide...
In This Guide...
This manual covers the Agilent 1290 Infinity Flexible Cube (G4227A)
1 Introduction
This chapter gives an introduction to the module, instrument overview and
internal connectors
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Installing the Module
This chapter gives information about the preferred stack setup for your system
and the installation of your Flexible Cube.
4 LAN Configuration
This chapter provides information on connecting the module to the Agilent
Data System.
5 Using the Module
This chapter provides information on how to set up the Flexible Cube for an
analysis and explains the basic settings.
6 Optimizing the Autosampler with the Flexible Cube
This chapter provides information on how to optimize the Autosampler and
the Flexible Cube for a minimum carry over and fastest cycle times.
7 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.

4 1290 Infinity Flexible Cube User Manual
In This Guide...
8 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
9 Maintenance and Repair
This chapter describes the maintenance of the module.
10 Parts and Materials for Maintenance
This chapter provides information on parts and material required for the
module.
11 Identifying Cables
This chapter provides information on cables used with the 1290 series of
HPLC modules.
12 Appendix
This chapter provides addition information on safety, legal and web.

1290 Infinity Flexible Cube User Manual 5
Contents
Contents
1 Introduction 9
Features 10
Overview of the Module 11
Flexible Cube Principle 12
Early Maintenance Feetback 19
Instrument Layout 20
Electrical Connections 21
Interfaces 23
2 Site Requirements and Specifications 29
Site Requirements 30
Physical Specifications 33
Performance Specifications 34
3 Installing the Module 37
Unpacking the Module 38
Optimizing the Stack Configuration 40
Installing the Module 47
Flow Connections to the Module 49
4 LAN Configuration 51
LAN-Configuration 52
5 Using the Module 53
Setting up the Flexible Cube with Agilent ChemStation 54
6 Optimizing the Autosampler with the Flexible Cube 61
Preparing the Autosampler and Flexible Cube 62
How to Achieve Lowest Carry Over 63
How to Achieve Highest Throughput Using the Flexible Cube 72

6 1290 Infinity Flexible Cube User Manual
Contents
7 Troubleshooting and Diagnostics 73
Overview of the Module’s Indicators and Test Functions 74
Status Indicators 75
User Interfaces 77
Agilent Lab Advisor Software 78
8 Error Information 79
What Are Error Messages 80
General Error Messages 81
Module Spezific Error Messages 88
9 Maintenance and Repair 91
Introduction to Maintenance 92
Warnings and Cautions 93
Overview of Maintenance 95
Cleaning the Module 96
Exchange Flush Pump Inlet Valve 97
Exchange Flush Pump Outlet Valve 100
Exchange Valve Rotor Seal 103
Replacing Module Firmware 107
10 Parts and Materials for Maintenance 109
Capillaries 110
Parts overview 111
Accessory Kits 112
11 Identifying Cables 113
Cable Overview 114
Analog Cables 116
Remote Cables 118
BCD Cables 121
CAN/LAN Cables 123
External Contact Cable 124
Agilent Module to PC 125

1290 Infinity Flexible Cube User Manual 7
Contents
12 Appendix 127
General Safety Information 128
The Waste Electrical and Electronic Equipment (WEEE) Directive
(2002/96/EC) 131
Radio Interference 132
Sound Emission 133
Flow Cell 134
Agilent Technologies on Internet 135

8 1290 Infinity Flexible Cube User Manual
Contents

9
1290 Infinity Flexible Cube User Manual
Agilent Technologies
1
Introduction
Features 10
Overview of the Module 11
Flexible Cube Principle 12
Early Maintenance Feetback 19
Instrument Layout 20
Electrical Connections 21
Serial Number Information (ALL) 22
Rear View of the Module 22
Interfaces 23
Overview Interfaces 24
This chapter gives an introduction to the module, instrument overview and
internal connectors

10 1290 Infinity Flexible Cube User Manual
1Introduction
Features
Features
The Agilent 1290 Infinity Flexible Cube introduces an unprecedented
flexibility to the Agilent 1290 Infinity System. It includes options to reduce
Carry Over and will later, with addition of valve pods, be able to convert the
G4226A Autosampler for fixed loop injections or be used as a multipurpose
valve box.
The G4227A Flexible Cube is capable of reducing carry over to a minimum and
maintains that level between the maintenance intervals of the Autosampler.
For specifications, see “Performance Specifications” on page 34.
NOTE This 1290 Infinity Flexible Cube has been introduced as part of the Agilent 1290 Infinity
Liquid Chromatograph.