Agilent Technologies InfinityLab LC Series User manual

1260 Infinity II Flexible Pumps
Agilent InfinityLab LC Series
User Manual
1260 Infinity II Flexible Pump User Manual

1260 Infinity II Flexible Pump User Manual
Notices
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Document No: SD-29000018 Rev. C
Edition: 6/2021
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© Agilent Technologies, Inc. 2021
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1260 Infinity II Flexible Pump User Manual 3
In This Guide...
This manual covers:
•Agilent 1260 Infinity II Flexible Pump (G7104C)
•Agilent 1260 Infinity II Bio Flexible Pump (G7131C)
1Introduction
This chapter gives an introduction to the module, instrument overview and internal
connectors.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical and
performance specifications.
3UsingtheModule
This chapter explains the operational parameters of the Agilent 1260 Infinity II
Flexible Pumps.
4PreparingthePump
This chapter provides information on how to set up the module for an analysis and
explains the basic settings.
5 How to Optimize the Performance of Your Module
This chapter gives hints on how to optimize the performance or use additional
devices.
6Troubleshootingand Diagnostics
Overview about the troubleshooting and diagnostic features.

1260 Infinity II Flexible Pump User Manual 4
7 Error Information
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.
8 Maintenance
This chapter describes the maintenance of the Agilent 1260 Infinity II Flexible
Pumps.
9 Parts and Materials for Maintenance
This chapter provides information on parts for maintenance.
10 Identifying Cables
This chapter provides information on cables used with the modules.
11 Hardware Information
This chapter describes the pump in more detail on hardware and electronics.
12 LAN Configuration
This chapter provides information on connecting the module to the controller
software.
13 Appendix
This chapter provides additional information on safety, legal and web.

1260 Infinity II Flexible Pump User Manual 5
Contents
1Introduction9
Agilent 1260 Infinity II Flexible Pump 10
Agilent 1260 Infinity II Bio Flexible Pump 12
Operating Principle 14
Positions of the Multi Purpose Valve 16
Leak and Waste Handling 20
2 Site Requirements and Specifications 25
Site Requirements 26
Physical Specifications 29
Performance Specifications 31
3UsingtheModule37
Magnets 38
Turn on/off 39
Status Indicators 41
Best Practices 42
Flushing the Filter 50
Purging the Pump 53
Recommendations for Bio-Compatible and Bio-Inert System 55
Recommendations for Degassers 57
Solvent Information 58
Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and
1260 Infinity II Flexible Pumps 65
Algae Growth in HPLC Systems 70
Setting up the Pump with the Instrument Control Interface 71
Agilent Local Control Modules 83
4 Preparing the Pump 84
Capillary Coding Guide 85
Installing Capillaries 88

1260 Infinity II Flexible Pump User Manual 6
5 How to Optimize the Performance of Your Module 92
Delay Volume and Extra-Column Volume 93
How to Configure the Optimum Delay Volume 94
How to Achieve Higher Resolution 96
Using Solvent Calibration Tables 99
6 Troubleshooting and Diagnostics 105
User Interfaces 106
Agilent Lab Advisor Software 107
Pump Leak Rate Test 108
System Pressure Test 113
Pump Self Test 115
7 Error Information 119
What Are Error Messages 121
General Error Messages 122
Pump Error Messages 130
8 Maintenance 149
Introduction to Maintenance 151
Warnings and Cautions 153
Overview of Maintenance 154
Cleaning the Module 155
Install Fittings and Capillaries 156
Remove and Install Doors 157
Replace the Pressure Sensor 159
Replace the Inlet Weaver 161
Replace the Inlet Valve (G7104C) 163
Replace the Inlet Valve (G7131C) 164
Remove the Jet Weaver 165
Install the Jet Weaver 167
Replace the Seal Wash Pump Cartridge 169
Replace the Multi-Channel Gradient Valve (MCGV) 170
Replace the Four-Channel Gradient Valve (4CGV) 172

1260 Infinity II Flexible Pump User Manual 7
Release a Stuck Inlet Valve 174
Remove the Pump Head Assembly (G7104C) 176
Remove the Pump Head Assembly (G7131C) 178
Pump Head Maintenance (Tool Free) 181
Install the Pump Head Assembly (G7104C) 206
Install the Pump Head Assembly (G7131C) 208
Replace the Outlet Valve (G7104C) 210
Replace the Outlet Valve (G7131C) 213
Replace the Multipurpose Valve 215
Replace Parts of the Multipurpose Valve (G7104C) 217
Replace Parts of the Multipurpose Valve (G7131C) 218
Replace the High Pressure Outlet Filter or Filter Frit 219
Install the Inline Filter 221
Remove the Inline Filter 223
Replace Parts of the Inline Filter 224
Replace the Seal Wash Sensor 226
Replace the Module Firmware 228
Prepare the Pump Module for Transport 229
9 Parts and Materials for Maintenance 231
Overview of Main Assemblies (G7104C) 232
Overview of Main Assemblies (G7131C) 234
Flow Connections (G7104C) 236
Flow Connections (G7131C) 238
Pump Heads (G7104C) 240
Pump Heads (G7131C) 244
Multipurpose Valve (G7104C) 247
Multipurpose Valve (G7131C) 248
Cover Parts 249
Accessory Kit (G7104C) 250
Accessory Kit (G7131C) 251
Tool Kit 252

1260 Infinity II Flexible Pump User Manual 8
10 Identifying Cables 253
Cable Overview 254
Analog Cables 256
Remote Cables 258
CAN/LAN Cables 262
Agilent Module to PC 263
USB Cables 264
11 Hardware Information 265
Firmware Description 266
Electrical Connections 269
Interfaces 271
Setting the 8-bit Configuration Switch 276
Early Maintenance Feedback 279
Instrument Layout 280
12 LAN Configuration 281
What You Have To Do First 282
TCP/IP Parameter Configuration 283
Configuration Switch 284
Initialization Mode Selection 285
Dynamic Host Configuration Protocol (DHCP) 287
Link Configuration Selection 290
Manual Configuration 291
PC and User Interface Software Setup 296
13 Appendix 298
General Safety Information 299
Waste Electrical and Electronic Equipment Directive 305
Radio Interference 306
Sound Emission 307
Agilent Technologies on Internet 308

1260 Infinity II Flexible Pump User Manual 9
1Introduction
Agilent 1260 Infinity II Flexible Pump 10
Product Description 10
Features 11
Agilent 1260 Infinity II Bio Flexible Pump 12
Product Description 12
Features 13
Operating Principle 14
Positions of the Multi Purpose Valve 16
Leak and Waste Handling 20
Leak Sensor 23
Waste Concept 24
This chapter gives an introduction to the module, instrument overview and
internal connectors.

1Introduction
Agilent 1260 Infinity II Flexible Pump
1260 Infinity II Flexible Pump User Manual 10
Agilent 1260 Infinity II Flexible Pump
Product Description
The new Agilent 1260 Infinity II Flexible Pump improves your everyday efficiency
by combining the performance of a high-pressure mixing UHPLC pump with the
flexibility of a low-pressure mixing UHPLC pump.
The power range of 5 mL/min with a maximum pressure up to 800 bar allows to
run HPLC and UHPLC methods on the same LC system. Intelligent System
Emulation Technology enables the transfer from existing methods from different
LC systems - current Agilent systems as well as instruments from other
manufacturers.
The Agilent Inlet Weaver mixer, active damping, or the optional Agilent Jet
Weaver mixers for additional mixing capacity achieve high analytical efficiency.
The established multipurpose valve enhances laboratory efficiency by adding
useful functionalities, for example, mixer in/out switch, filter backflush, or
automatic purge.
BlendAssist software simplifies your workflow with accurate buffer/additive
blending.
Figure 1 Overview of the Flexible Pump
Status indicator
Pump head
MCGV
Inlet weaver
Power switch
Leak drain
Pressure sensor

1260 Infinity II Flexible Pump User Manual 11
1Introduction
Agilent 1260 Infinity II Flexible Pump
Features
•High accuracy and precision for solvent flow and composition.
•Maximum backpressure up to 800 bar and high analytical flow rates up to
5 mL/min for high chromatographic performance.
•Integrated high efficiency degasser with low internal volume is based on PTFE
AF technology and has a fast change-over of solvents for purging the pump.
•High solvent mixing efficiency before reaching the pump head due to the
established Inlet Weaver technology.
•Active damping with independently controllable high resolution pump drives
and firmware-embedded tuning algorithms significantly reduce ripples and
associated UV noise.
•SwitchableJetWeavermixers(35µL,100µL,380µL)forincreasedmixing
performance.
•A multipurpose valve enables automatic software-embedded functionalities
such as switching the optional mixer in and out, or automatic purging.
•The unique Intelligent System Emulation Technology (ISET) enables the
emulation of existing Agilent 1100, 1200, and 1260 Infinity Series instruments,
as well as Waters Alliance, Waters H-Class, and Shimadzu Prominence
instruments.
•Precise and accurate buffer/additive blending using the BlendAssist software
feature, implemented in the pump driver.
•Built-in active seal-wash for increased uptime.

1Introduction
Agilent 1260 Infinity II Bio Flexible Pump
1260 Infinity II Flexible Pump User Manual 12
Agilent 1260 Infinity II Bio Flexible Pump
Product Description
The 1260 Infinity II Bio Flexible Pump is a UHPLC pump comprising
biocompatible components, developed for biopharma applications and for other
analyses requiring high-salt and extreme-pH conditions. This pump enables
quaternary solvent delivery at pressures up to 800 bar and flow rates up to
5mL/min.
The 1260 Infinity II Bio Flexible Pump combines high performance and simplified
operation with remarkable flexibility in automated gradient formation and solvent
blending. This pump also facilitates seamless method transfer using Intelligent
System Emulation Technology (ISET) and automated buffer blending.
Figure 2 Overview of the Flexible Pump
Status indicator
Pump head
MCGV
Inlet weaver
Power switch
Leak drain
Pressure sensor

1260 Infinity II Flexible Pump User Manual 13
1Introduction
Agilent 1260 Infinity II Bio Flexible Pump
Features
•Biocompatible wetted parts in the solvent delivery unit ensure integrity of
biomolecules and minimize unwanted surface interaction while increasing
column lifetime
•Biocompatible pump is resistant to corrosion from high salt concentrations
and harsh cleaning procedures for increased instrument uptime, while the
wide pH range offers increased flexibility
•Low delay volume, extended pressure range up to 800 bar, analytical flow
rates up to 5 mL/min, and superior quaternary gradient mixing enable higher
precision and accuracy for maximum chromatographic performance
•Agilent Buffer Advisor Software provides a fast and simple way to create salt
and pH gradients, eliminating the tedious and error-prone method
development steps of buffer preparation, buffer blending, and pH scouting
•Built-in seal wash keeps maintenance to a minimum, increases longevity, and
reduces operating cost
•BlendAssist, a software feature in the pump driver, enables convenient,
precise, and accurate buffer or additive blending
•ISET enables emulation of existing Agilent 1100, 1200, and 1260 Infinity
Series instruments, as well as Waters Alliance, Waters H-Class, and Shimadzu
Prominence instruments, facilitating seamless method transfer

1Introduction
Operating Principle
1260 Infinity II Flexible Pump User Manual 14
Operating Principle
The pump head comprises two pump chambers in series with independent
high-resolution motion control. A pressure sensor in the flow path monitors the
pressure. The pump control uses this signal for minimizing the pressure ripple in
order to achieve highest flow precision. A stable flow can be delivered even in
case of eventual small internal leaks, which can be compensated automatically.
A heat exchanger between two pump chambers strongly reduces thermal effects
due to solvent compression under very high pressures.
As solvents are compressed by the pump head and expand further down the flow
path, for example in the column, the volumetric flow is changed depending on the
compressibility of the liquid. Agilent control software allows specifying pure
solvents, pre-mixed solvents and solvent gradients. Associated Agilent solvent
libraries are used by the pump control for enhanced flow accuracy, which is
required for cross-instrument or cross-system reproducibility and method
compatibility.
A high resolution encoder unit is attached to the pump drives, which divides a
single turn into 65000 steps. Each step corresponds to a volume of about 300 pL,
which allows an extremely precise control.

1260 Infinity II Flexible Pump User Manual 15
1Introduction
Operating Principle
Figure 3 The hydraulic path (example of G7104A Flexible Pump installed with a G7167B
Multisampler)
Pump head
Inlet weaver
MCGV
Pressure sensor
Multisampler
Seal wash pump
Degasser
Seal wash sensor
Multipurpose
valve
Jet
Weaver

1Introduction
Positions of the Multi Purpose Valve
1260 Infinity II Flexible Pump User Manual 16
Positions of the Multi Purpose Valve
The Multipurpose Valve allows easy software controlled switching between
different modes of operation.
Normal Operating Mode Without Mixer
In normal operating mode, the flow comes from the pump head, passes the
pressure sensor and arrives at the central port of the Multipurpose Valve. The
flow leaves the valve through port 4 to the system (autosampler etc.).
Figure 4 Valve position in normal operating mode without mixer
From pump
To system

1260 Infinity II Flexible Pump User Manual 17
1Introduction
Positions of the Multi Purpose Valve
Purge Mode
In purge mode, the flow is diverted to the waste container.
Figure 5 Valve position in purge mode
From pump
To waste

1Introduction
Positions of the Multi Purpose Valve
1260 Infinity II Flexible Pump User Manual 18
Normal Operating Mode With Jet Weaver and Optional Inline Filter
In this mode, the flow passes an optional Jet Weaver and the optional inline filter.
This configuration is recommended for special applications which require an
increased mixing efficiency.
Figure 6 Valve position in normal operating mode with Jet Weaver
From pump
To system
Inline filter
Jet Weaver

1260 Infinity II Flexible Pump User Manual 19
1Introduction
Positions of the Multi Purpose Valve
Filter Flush Mode
This mode is used for cleaning the inline filter by back-flushing it. The flow goes
to port 5, passes the inline filter in opposite direction and leaves to the waste
through port 7.
Figure 7 Valve position in filter flush mode
From pump
Inline filter
To waste
CAUTION
Damage to the valve
Use the filter flush mode only if the optional inline filter is installed.

1Introduction
Leak and Waste Handling
1260 Infinity II Flexible Pump User Manual 20
Leak and Waste Handling
The Agilent InfinityLab LC Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
The solvent cabinet is designed to store a maximum volume of 8 L solvent. The
maximum volume for an individual bottle stored in the solvent cabinet should not
exceed 2 L. For details, see the usage guideline for the Agilent Infinity II Solvent
Cabinets (a printed copy of the guideline has been shipped with the solvent
cabinet, electronic copies are available on the Internet).
All leak plane outlets are situated in a consistent position so that all Infinity and
Infinity II modules can be stacked on top of each other. Waste tubes are guided
through a channel on the right hand side of the instrument, keeping the front
access clear from tubes.
The leak plane provides leak management by catching all internal liquid leaks,
guiding them to the leak sensor for leak detection, and passing them on to the
next module below, if the leak sensor fails. The leak sensor in the leak plane
stops the running system as soon as the leak detection level is reached.
Solvent and condensate is guided through the waste channel into the waste
container:
•from the detector's flow cell outlet
•from the Multisampler needle wash port
•from the Sample Cooler or Sample Thermostat (condensate)
•from the pump's Seal Wash Sensor (if applicable)
•from the pump's Purge Valve or Multipurpose Valve