Agilent Technologies 4150 TapeStation system User guide

Agilent Technologies
Agilent 4150 TapeStation
System
System Manual

Agilent 4150 TapeStation System Manual
Notices
© Agilent Technologies, Inc. 2018
No part of this manual may be reproduced
in any form or by any means (including
electronic storage and retrieval or transla-
tion into a foreign language) without prior
agreement and written consent from Agi-
lent Technologies, Inc. as governed by
United States and international copyright
laws.
Manual Part Number
G2992-90000
Edition
12/2018
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
The material contained in this docu-
ment is provided “as is,” and is sub-
ject to being changed, without notice,
in future editions. Further, to the max-
imum extent permitted by applicable
law, Agilent disclaims all warranties,
either express or implied, with regard
to this manual and any information
contained herein, including but not
limited to the implied warranties of
merchantability and fitness for a par-
ticular purpose. Agilent shall not be
liable for errors or for incidental or
consequential damages in connection
with the furnishing, use, or perfor-
mance of this document or of any
information contained herein. Should
Agilent and the user have a separate
written agreement with warranty
terms covering the material in this
document that conflict with these
terms, the warranty terms in the sep-
arate agreement shall control.
Technology Licenses
The hardware and/or software described in
this document are furnished under a
license and may be used or copied only in
accordance with the terms of such license.
Restricted Rights Legend
If software is for use in the performance of
a U.S. Government prime contract or sub-
contract, Software is delivered and licensed
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or as a “commercial item” as defined in
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software” as defined in FAR 52.227-19
(June 1987) or any equivalent agency regu-
lation or contract clause. Use, duplication
or disclosure of Software is subject to Agi-
lent Technologies’ standard commercial
license terms, and non-DOD Departments
and Agencies of the U.S. Government will
receive no greater than Restricted Rights as
defined in FAR 52.227-19(c)(1-2) (June
1987). U.S. Government users will receive
no greater than Limited Rights as defined in
FAR 52.227-14 (June 1987) or DFAR
252.227-7015 (b)(2) (November 1995), as
applicable in any technical data.
Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully
understood and met.

Agilent 4150 TapeStation System Manual 3
In this Guide...
In this Guide...
This manual covers the 4150 TapeStation system
1Safety
This chapter provides information on safety symbols and safety messages.
2 Legal and Regulatory
This chapter provides additional information on legal and regulatory aspects.
3 Product Description
This chapter gives an introduction to the instrument and provides information
on intended use and instrument specifications.
4 Installation
This chapter gives information about the installation of the system.
5TapeStationSoftware
This chapter gives an overview on the three packages within the TapeStation
software.
6 Operating Instructions
This chapter explains the operation of the 4150 TapeStation system.
7 Good Measurement Practices
This chapter summarizes information on good measurement practices for
ScreenTape assays.
8 Instrument Diagnosis
This chapter contains information on instrument diagnosis, available tests,
and reporting.

4 Agilent 4150 TapeStation System Manual
In this Guide...
9 Maintenance
This chapter describes the maintenance of the 4150 TapeStation system.
10 Troubleshooting
This chapter gives an overview about the troubleshooting features of the 4150
TapeStation system. It includes information about application troubleshooting
as well.
11 Appendix
This chapter contains parts information.

Agilent 4150 TapeStation System Manual 5
Contents
Contents
1Safety 9
Symbols 10
Safety Messages 12
General Safety Information 13
2 Legal and Regulatory 15
Sound Emission 16
Waste Electrical and Electronic Equipment (WEEE) Directive 17
3 Product Description 19
Intended use of the 4150 TapeStation System 20
Overview of the System and ScreenTape Architecture 21
Product Descriptions 25
Physical Specification 32
4 Installation 33
Site Requirements 34
Bench Space Required 36
Unpacking 38
Initial Setup of the System 45
Prepare the Working Area 48
5 TapeStation Software 51
TapeStation Controller Software 52
TapeStation Analysis Software 58
Agilent Information Center 60

6 Agilent 4150 TapeStation System Manual
Contents
6 Operating Instructions 63
Sample Preparation 64
Preparing the Instrument 65
Starting the Analysis 73
Analysis End 77
Follow Up 78
7 Good Measurement Practices 81
Use of Correct Tools 83
Tools and Handling 84
Good Pipetting Practice 85
Reagents and Reagent Mixes 86
ScreenTape Devices 87
Partially Used ScreenTape Devices 90
Sample Preparation 91
Ladder Considerations 92
Use the Correct Protocol 93
Mixing Recommendations 94
Centrifugation Recommendations 97
RNA Assays 99
Peak Integration 101
Peak Maxima Versus Average Molecular Weight Sizing and Molarity 104
Sizing Range 106
Quantitative range 107
Maximum Buffer Concentration in Sample 110
RINe Functional Range 112
8 Instrument Diagnosis 113
Instrument Diagnosis 114
Tests 115
Reporting 122

Agilent 4150 TapeStation System Manual 7
Contents
9 Maintenance 123
Maintenance Information 124
Exchange the Piercing Needle 127
Electrode Cartridge 130
Cleaning the Instrument 146
Preparing the Instrument for Transportation 147
Boxing for Transportation 153
Assembling Nest Covers 156
Insertion of the Lower Nest Cover 158
10 Troubleshooting 165
Software Messages 167
Troubleshooting DNA Applications 178
Troubleshooting RNA Applications 200
Instrument Communication 212
Collecting Troubleshooting Information 221
11 Appendix 223
Accessories Shipment List 224

8 Agilent 4150 TapeStation System Manual
Contents

9
Agilent 4150 TapeStation System Manual
Agilent Technologies
1
Safety
Symbols 10
Safety Messages 12
General Safety Information 13
This chapter provides information on safety symbols and safety messages.

10 Agilent 4150 TapeStation System Manual
1 Safety
Symbols
Symbols
Table 1 Symbols
The apparatus is marked with this symbol when the user should refer to
the instruction manual in order to protect risk of harm to the operator and
to protect the apparatus against damage.
Indicates dangerous voltages.
Indicates a protected ground terminal.
Confirms that a manufactured product complies with all applicable
European Community directives. The European Declaration of Conformity
is available at:
http://regulations.corporate.agilent.com/DoC/search.htm
Manufacturing date.
Power symbol indicates On/Off.
The apparatus is not completely disconnected from the mains supply
when the power switch is in the Off position
Pacemaker
Magnets could affect the functioning of pacemakers and implanted heart
defibrillators.
A pacemaker could switch into test mode and cause illness. A heart
defibrillator may stop working. If you wear these devices keep at least
55 mm distance to magnets. Warn others who wear these devices from
getting too close to magnets.
Magnetic field
Magnets produce a far-reaching, strong magnetic field. They could
damage TVs and laptops, computer hard drives, credit and ATM cards,
data storage media, mechanical watches, hearing aids and speakers.
Keep magnets at least 25 mm away from devices and objects that could
be damaged by strong magnetic fields.

Agilent 4150 TapeStation System Manual 11
Safety
1
Symbols
Indicates a pinching or crushing hazard
Indicates a piercing or cutting hazard.
Table 1 Symbols

12 Agilent 4150 TapeStation System Manual
1 Safety
Safety Messages
Safety Messages
WARNING
A WARNING
alerts you to situations that could cause physical injury or death.
➔Do not proceed beyond a warning until you have fully understood and met the
indicated conditions.
CAUTION
A CAUTION
alerts you to situations that could cause loss of data, or damage of equipment.
➔Do not proceed beyond a caution until you have fully understood and met the
indicated conditions.

Agilent 4150 TapeStation System Manual 13
Safety
1
General Safety Information
General Safety Information
The following general safety precautions must be observed during all phases of
operation, service, and repair of this instrument. Failure to comply with these
precautions or with specific warnings elsewhere in this manual violates safety
standards of design, manufacture, and intended use of the instrument. Agilent
Technologies assumes no liability for the customer’s failure to comply with
these requirements.
WARNING
Ensure the proper usage of the equipment.
The protection provided by the equipment may be impaired.
➔The operator of this instrument is advised to use the equipment in a manner as
specified in this manual.
WARNING
Wrong voltage range, frequency or cabling
Personal injury or damage to the instrument
➔Verify that the voltage range and frequency of your power distribution matches to
the power specification of the individual instrument.
➔Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
➔Make all connections to the unit before applying power.

14 Agilent 4150 TapeStation System Manual
1 Safety
General Safety Information
WARNING
Missing electrical ground
Electrical shock
➔If your product is provided with a grounding type power plug, the instrument
chassis and cover must be connected to an electrical ground to minimize shock
hazard.
➔The ground pin must be firmly connected to an electrical ground (safety ground)
terminal at the power outlet. Any interruption of the protective (grounding)
conductor or disconnection of the protective earth terminal will cause a potential
shock hazard that could result in personal injury.
WARNING
Instrument covers removed
Electrical shock
➔Do Not Remove the Instrument Cover
➔Only Agilent authorized personnel are allowed to remove instrument covers.
Always disconnect the power cables and any external circuits before removing the
instrument cover.
WARNING
Damage to the instrument
Personal injury (for example electrical shock, intoxication)
➔Instruments that appear damaged or defective should be made inoperative and
secured against unintended operation until they can be repaired by qualified
service personnel.

15
Agilent 4150 TapeStation System Manual
Agilent Technologies
2
Legal and Regulatory
Sound Emission 16
Waste Electrical and Electronic Equipment (WEEE) Directive 17
This chapter provides additional information on legal and regulatory aspects.

16 Agilent 4150 TapeStation System Manual
2 Legal and Regulatory
Sound Emission
Sound Emission
Manufacturer’s Declaration
This statement is provided to comply with the requirements of the German
Sound Emission Directive of 18 January 1991.
This product has a sound pressure emission (at the operator position) <70 dB.
•Sound Pressure Lp <70 dB (A)
•At Operator Position
•Normal Operation
•According to ISO 7779:1988/EN 27779/1991 (Type Test)

Agilent 4150 TapeStation System Manual 17
Legal and Regulatory
2
Waste Electrical and Electronic Equipment (WEEE) Directive
Waste Electrical and Electronic Equipment (WEEE) Directive
This product complies with the European WEEE Directive marking
requirements. The affixed label indicates that you must not discard this
electrical/electronic product in domestic household waste.
NOTE
Do not dispose of in domestic household waste
To return unwanted products, contact your local Agilent office, or see
http://www.agilent.com for more information.

18 Agilent 4150 TapeStation System Manual
2 Legal and Regulatory
Waste Electrical and Electronic Equipment (WEEE) Directive

19
Agilent 4150 TapeStation System Manual
Agilent Technologies
3
Product Description
Intended use of the 4150 TapeStation System 20
Overview of the System and ScreenTape Architecture 21
Overview of the System 21
ScreenTape Architecture 24
Product Descriptions 25
Physical Specification 32
This chapter gives an introduction to the instrument and provides information
on intended use and instrument specifications.

20 Agilent 4150 TapeStation System Manual
3 Product Description
Intended use of the 4150 TapeStation System
Intended use of the 4150 TapeStation System
The 4150 TapeStation system is intended to separate nucleic acids by means
of electrophoresis. The system detects:
•Fluorescently stained double stranded DNA including genomic DNA
•Fluorescently stained total RNA (Eukaryotic and Prokaryotic)
The 4150 TapeStation system is designed for professional use to exclusively
run Agilent ScreenTape kits and specified consumables.
For Research Use Only. Not for use in diagnostic procedures.
Do not use this product in any manner not specified by the manufacturer. The
protective features of this product may be impaired if it is used in a manner
not specified in the operation instructions.