Agilent Technologies 4150 TapeStation system User guide

Agilent Technologies
Agilent 4150 TapeStation
System
System Manual

Agilent 4150 TapeStation System Manual
Notices
© Agilent Technologies, Inc. 2018
No part of this manual may be reproduced
in any form or by any means (including
electronic storage and retrieval or transla-
tion into a foreign language) without prior
agreement and written consent from Agi-
lent Technologies, Inc. as governed by
United States and international copyright
laws.
Manual Part Number
G2992-90000
Edition
12/2018
Printed in Germany
Agilent Technologies
Hewlett-Packard-Strasse 8
76337 Waldbronn
Warranty
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ject to being changed, without notice,
in future editions. Further, to the max-
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either express or implied, with regard
to this manual and any information
contained herein, including but not
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liable for errors or for incidental or
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Technology Licenses
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully
understood and met.

Agilent 4150 TapeStation System Manual 3
In this Guide...
In this Guide...
This manual covers the 4150 TapeStation system
1Safety
This chapter provides information on safety symbols and safety messages.
2 Legal and Regulatory
This chapter provides additional information on legal and regulatory aspects.
3 Product Description
This chapter gives an introduction to the instrument and provides information
on intended use and instrument specifications.
4 Installation
This chapter gives information about the installation of the system.
5TapeStationSoftware
This chapter gives an overview on the three packages within the TapeStation
software.
6 Operating Instructions
This chapter explains the operation of the 4150 TapeStation system.
7 Good Measurement Practices
This chapter summarizes information on good measurement practices for
ScreenTape assays.
8 Instrument Diagnosis
This chapter contains information on instrument diagnosis, available tests,
and reporting.

4 Agilent 4150 TapeStation System Manual
In this Guide...
9 Maintenance
This chapter describes the maintenance of the 4150 TapeStation system.
10 Troubleshooting
This chapter gives an overview about the troubleshooting features of the 4150
TapeStation system. It includes information about application troubleshooting
as well.
11 Appendix
This chapter contains parts information.

Agilent 4150 TapeStation System Manual 5
Contents
Contents
1Safety 9
Symbols 10
Safety Messages 12
General Safety Information 13
2 Legal and Regulatory 15
Sound Emission 16
Waste Electrical and Electronic Equipment (WEEE) Directive 17
3 Product Description 19
Intended use of the 4150 TapeStation System 20
Overview of the System and ScreenTape Architecture 21
Product Descriptions 25
Physical Specification 32
4 Installation 33
Site Requirements 34
Bench Space Required 36
Unpacking 38
Initial Setup of the System 45
Prepare the Working Area 48
5 TapeStation Software 51
TapeStation Controller Software 52
TapeStation Analysis Software 58
Agilent Information Center 60

6 Agilent 4150 TapeStation System Manual
Contents
6 Operating Instructions 63
Sample Preparation 64
Preparing the Instrument 65
Starting the Analysis 73
Analysis End 77
Follow Up 78
7 Good Measurement Practices 81
Use of Correct Tools 83
Tools and Handling 84
Good Pipetting Practice 85
Reagents and Reagent Mixes 86
ScreenTape Devices 87
Partially Used ScreenTape Devices 90
Sample Preparation 91
Ladder Considerations 92
Use the Correct Protocol 93
Mixing Recommendations 94
Centrifugation Recommendations 97
RNA Assays 99
Peak Integration 101
Peak Maxima Versus Average Molecular Weight Sizing and Molarity 104
Sizing Range 106
Quantitative range 107
Maximum Buffer Concentration in Sample 110
RINe Functional Range 112
8 Instrument Diagnosis 113
Instrument Diagnosis 114
Tests 115
Reporting 122

Agilent 4150 TapeStation System Manual 7
Contents
9 Maintenance 123
Maintenance Information 124
Exchange the Piercing Needle 127
Electrode Cartridge 130
Cleaning the Instrument 146
Preparing the Instrument for Transportation 147
Boxing for Transportation 153
Assembling Nest Covers 156
Insertion of the Lower Nest Cover 158
10 Troubleshooting 165
Software Messages 167
Troubleshooting DNA Applications 178
Troubleshooting RNA Applications 200
Instrument Communication 212
Collecting Troubleshooting Information 221
11 Appendix 223
Accessories Shipment List 224

8 Agilent 4150 TapeStation System Manual
Contents

9
Agilent 4150 TapeStation System Manual
Agilent Technologies
1
Safety
Symbols 10
Safety Messages 12
General Safety Information 13
This chapter provides information on safety symbols and safety messages.

10 Agilent 4150 TapeStation System Manual
1 Safety
Symbols
Symbols
Table 1 Symbols
The apparatus is marked with this symbol when the user should refer to
the instruction manual in order to protect risk of harm to the operator and
to protect the apparatus against damage.
Indicates dangerous voltages.
Indicates a protected ground terminal.
Confirms that a manufactured product complies with all applicable
European Community directives. The European Declaration of Conformity
is available at:
http://regulations.corporate.agilent.com/DoC/search.htm
Manufacturing date.
Power symbol indicates On/Off.
The apparatus is not completely disconnected from the mains supply
when the power switch is in the Off position
Pacemaker
Magnets could affect the functioning of pacemakers and implanted heart
defibrillators.
A pacemaker could switch into test mode and cause illness. A heart
defibrillator may stop working. If you wear these devices keep at least
55 mm distance to magnets. Warn others who wear these devices from
getting too close to magnets.
Magnetic field
Magnets produce a far-reaching, strong magnetic field. They could
damage TVs and laptops, computer hard drives, credit and ATM cards,
data storage media, mechanical watches, hearing aids and speakers.
Keep magnets at least 25 mm away from devices and objects that could
be damaged by strong magnetic fields.