Agilent Technologies 1290 Infinity II User manual

Agilent Technologies
Agilent InfinityLab LC Series
1290 Infinity II
Preparative Open-Bed
Fraction Collector
User Manual

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
Notices
© Agilent Technologies, Inc. 2016, 2017
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Manual Part Number
G7159-90000 Rev. C
Edition
02/2017
Printed in Germany
Agilent Technologies
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
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hazard. It calls attention to an
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or the like that, if not correctly
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understood and met.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 3
In This Guide
In This Guide
This manual contains technical reference information about the Agilent 1290
Infinity II Preparative Open-Bed Fraction Collector (G7159B).
1Introduction
This chapter gives an introduction to the module and an instrument overview.
2 Site Requirements and Specifications
This chapter provides information on environmental requirements, physical
and performance specifications.
3 Using the Fraction Collector
This chapter explains the essential operational parameters of the module.
4 Preparing the Fraction Collector
This chapter explains the operational parameters of the module.
5 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
6 Error Information
This chapter describes the meaning of error messages, and provides
information on probable causes and suggested actions how to recover from
error conditions.
7 Maintenance
This chapter describes the maintenance of the module.
8 Parts for Maintenance and Repair
This chapter provides information on parts for maintenance and repair.

4 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
In This Guide
9 Identifying Cables
This chapter provides information on cables used with the module.
10 Hardware Information
This chapter describes the module in more detail on hardware and electronics.
11 Appendix
This chapter provides additional information on safety, legal, and web.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 5
Contents
Contents
1Introduction 9
Product Description 10
Features 11
Overview of the Module 12
Fraction Collector Principle 13
Leak and Waste Concept 14
2 Site Requirements and Specifications 15
Site Requirements 16
Physical Specifications 19
Performance Specifications 20
3 Using the Fraction Collector 23
Solvent Information 24
Turn on/off 26
Status Indicators 27
Drawer Status Indicator 28
Exchange Drawers 29
Exchange Containers 31
Replace Inlet/Waste Tubings 32
4 Preparing the Fraction Collector 37
Best Practices 38
Solvent Information 39
Capillary Color Coding Guide 45
Installing Capillaries 46
Setting up the Fraction Collector with the Instrument Control Interface 49
Method Parameter Settings 55
Pooling 63

6 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
Contents
5 Troubleshooting and Diagnostics 65
User Interfaces 66
Agilent Lab Advisor Software 67
6 Error Information 69
What Are Error Messages 70
General Error Messages 71
7 Maintenance 79
Introduction to Maintenance 80
Warnings and Cautions 81
Cleaning the Module 83
Overview of Maintenance 84
Install and Remove the Top Fume Hood 85
Clean the Leak Pan 87
Prepare the Module for Transportation 88
Replace the Module Firmware 90
8 Parts for Maintenance and Repair 91
Supported Containers 92
List of Recommended Fraction Tubes 93
Fraction Collector Accessory Kit 94
Tubing Kits 95
Top Fume Hood Kit 97
9 Identifying Cables 99
Cable Overview 100
Analog Cables 102
Remote Cables 104
CAN/LAN Cables 108
RS-232 Cables 109
USB 110

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 7
Contents
10 Hardware Information 111
Firmware Description 112
Electrical Connections 115
Interfaces 118
Setting the 6-bit Configuration Switch 126
Early Maintenance Feedback 130
Instrument Layout 131
11 Appendix 133
General Safety Information 134
Waste Electrical and Electronic Equipment Directive 140
Radio Interference 141
Sound Emission 142
Agilent Technologies on Internet 143

8 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
Contents

9
1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
Agilent Technologies
1
Introduction
Product Description 10
Features 11
Overview of the Module 12
Fraction Collector Principle 13
Leak and Waste Concept 14
This chapter gives an introduction to the module and an instrument overview.

10 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
1 Introduction
Product Description
Product Description
The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector is the
ideal solution that automatically manages high-capacity fraction collection of
purified peaks for semi-preparative and preparative scale purification. Collect
milligrams to multi-gram quantities of sample intuitive with ease. The Agilent
1290 Infinity II Preparative Open-Bed Fraction Collector features handling a
wide variety of collection vessels with ease and capacity of up to 5.9 L by using
150 x 25 mm (L x OD). The containers use identification tags to automatically
detect the vessel dimensions. With a novel robotic technology, Y-, Z-, theta-axis
probe functionality minimizes sample carryover between the fractions during
movements between the vessels. The Agilent 1290 Infinity II Preparative
Open-Bed Fraction Collector is optimized for flow rates up to 200 mL/min,
and can be easily integrated with any Agilent solvent delivery module,
autosampler, or detector.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 11
Introduction
1
Features
Features
•Maximize your flexibility – Choose how much you want to collect, whether
you prefer a large number of fractions which enables collection of up to
432 fraction in 150 x 12 mm (L x OD) vessels, or you prefer large volumes,
or any combination of formats. Fraction Containers are available in eight
formats, and you mix them to accommodate the different vessel ODs.
•High capacity – Using one type of drawer and offering six different type of
containers, the Agilent 1290 Infinity II Preparative Open-Bed Fraction
Collector can collect a maximum of 5.9 L.
•Scalable collection volumes – The Agilent 1290 Infinity II Preparative
Open-Bed Fraction Collector enhances flexibility by providing two
differently optimized tubing kits. Optimized for collections up to 50 mL/min
and the other high flow kit for up to 200 mL/min.
•Accurate collection modes – real time peak or mass detection algorithms
allow to achieve accurate, reproducible fraction detection every time.
•Lowest delay volumes - Optimized tubing and unique delay sensor for
accurate collection minimize peak dispersion.
•Low carryover - The Agilent 1290 Infinity II Preparative Open-Bed
Fraction Collector is designed for low carryover due to extremely fast
Y-, Z-, and Theta-axis movement, thus minimizing sample loss during
movements from vessel-to-vessel.

12 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
1 Introduction
Overview of the Module
Overview of the Module
The Fraction Collector transport mechanism uses a cartesian robot. The Y
drive together with the Theta drive optimize the positioning for the fraction
containers. The robot moves above a position and the diverter valve switches
to collect in the according collection position.
All axes of the transport mechanism are driven by very fast BLCD motors.
Optical encoders ensure the correct operation of the movement.
The entire flow path including the diverter valve and delay sensor are always
flushed by the mobile phase for minimum internal carry-over.
To reduce carry-over further, you have the possibility to rinse the diverter
valve on a rinse port. The bottle containing the mobile phase for the wash
procedure will be located in the solvent bottle cabinet. The rinse waste
produced during this operation is channeled safely away through a waste
drain.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 13
Introduction
1
Fraction Collector Principle
Fraction Collector Principle
The movements of the Fraction Collector components during the sequence are
monitored continuously by the Fraction Collector processor. The processor
defines specific time windows and mechanical ranges for each movement. If a
specific step of the sequence is not completed successfully, an error message is
generated.
The standard fractioning sequence occurs in the following order:
1The Fraction Collector starts always from the home position.
2When the sample is injected, the fraction probe with diverter valve moves to
the required position.
3When the trigger is given by the detector, the diverter valve opens to collect
the fraction.
4When the trigger is given by the detector, the diverter valve closes and the
arm moves to the next fraction position or back to the home position if this
function is chosen in the CDS.
Fractioning Sequence
Before the start of the sequence, and during an analysis, the diverter valve is
in the fraction start position. In this position, the mobile phase flows through
the diverter valve towards waste.

14 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
1 Introduction
Leak and Waste Concept
Leak and Waste Concept
1 Rinse Port 2 Drain Outlet

15
1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
Agilent Technologies
2
Site Requirements and Specifications
Site Requirements 16
Physical Specifications 19
Performance Specifications 20
This chapter provides information on environmental requirements, physical
and performance specifications.

16 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
2 Site Requirements and Specifications
Site Requirements
Site Requirements
A suitable environment is important to ensure optimal performance of the
instrument.
Power Considerations
The module power supply has wide ranging capability. It accepts any line
voltage in the range described in Table 1 on page 19. Consequently there is no
voltage selector in the rear of the module. There are also no externally
accessible fuses, because automatic electronic fuses are implemented in the
power supply.
WARNING
Hazard of electrical shock or damage of your instrumentation
can result, if the devices are connected to a line voltage higher than specified.
➔Connect your instrument to the specified line voltage only.
WARNING
The module is partially energized when switched off, as long as the power cord is
plugged in.
Repair work at the module can lead to personal injuries, e.g. electrical shock, when
the cover is opened and the module is connected to power.
➔Always unplug the power cable before opening the cover.
➔Do not connect the power cable to the instrument while the covers are removed.
WARNING
Inaccessible power plug.
In case of emergency it must be possible to disconnect the instrument from the
power line at any time.
➔Make sure the power connector of the instrument can be easily reached and
unplugged.
➔Provide sufficient space behind the power socket of the instrument to unplug the
cable.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 17
Site Requirements and Specifications
2
Site Requirements
Power Cords
Country-specific power cords are available for the module. The female end of
all power cords is identical. It plugs into the power-input socket at the rear.
The male end of each power cord is different and designed to match the wall
socket of a particular country or region.
Agilent makes sure that your instrument is shipped with the power cord that
is suitable for your particular country or region.
WARNING
Absence of ground connection
The absence of ground connection can lead to electric shock or short circuit.
➔Never operate your instrumentation from a power outlet that has no ground
connection.
WARNING
Unintended use of supplied power cords
Using power cords for unintended purposes can lead to personal injury or damage
of electronic equipment.
➔Never use a power cord other than the one that Agilent shipped with this
instrument.
➔Never use the power cords that Agilent Technologies supplies with this instrument
for any other equipment.
➔Never use cables other than the ones supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.
WARNING
Power cords
Solvents may damage electrical cables.
➔Prevent electrical cables from getting in contact with solvents.
➔Exchange electrical cables after contact with solvents.

18 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
2 Site Requirements and Specifications
Site Requirements
Bench Space
The module dimensions and weight (see Table 1 on page 19) allow you to place
the module on almost any desk or laboratory bench. It needs an additional
2.5 cm (1.0 inches) of space on either side and approximately 8 cm
(3.1 inches) in the rear for air circulation and electric connections.
If the bench shall carry a complete HPLC system, make sure that the bench is
designed to bear the weight of all modules.
The module should be operated in a horizontal position.
Condensation
NOTE
Agilent recommends that you install the HPLC instrument in the InfinityLab Flex Bench
rack. This option helps to save bench space as all modules can be placed into one single
stack. It also allows to easily relocate the instrument to another Lab.
CAUTION
Condensation within the module
Condensation can damage the system electronics.
➔Do not store, ship or use your module under conditions where temperature
fluctuations could cause condensation within the module.
➔If your module was shipped in cold weather, leave it in its box and allow it to warm
slowly to room temperature to avoid condensation.

1290 Infinity II Preparative Open-Bed Fraction Collector User Manual 19
Site Requirements and Specifications
2
Physical Specifications
Physical Specifications
Table 1 Physical Specifications
Type Specification Comments
Weight 30.6 kg
Dimensions
(height × width × depth)
781 mm x 393 mm x 622 mm
Line voltage 100 – 240 V~, ± 10 % Wide-ranging
capability
Line frequency 50 or 60 Hz, ± 5 %
Power consumption 350 VA, 350 W
Ambient operating
temperature
4-40°C (39.2 -104°F)
Ambient non-operating
temperature
-40 – 70 °C (-40 – 158 °F)
Humidity ≤80 % r.h. up to 31 °C, decreasing to
50 % r.h. at 40 °C
Non-condensing
Operating altitude Up to 3000 m (9842 ft)
Non-operating altitude Up to 4600 m (15092 ft) For storing the module
Safety standards:
IEC, EN, CSA, UL
Installation category II, Pollution degree 2 For indoor use only.
ISM Classification ISM Group 1 Class B According to CISPR 11

20 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual
2 Site Requirements and Specifications
Performance Specifications
Performance Specifications
Table 2 Performance Specifications
Type Specification Comment
Delay volume Calculated by Lab Advisor. Dead
volume in Valve tip appr. 30 µL
Time to move from vessel
to vessel
0.3 s
Minimum system flow 1 mL/min
Maximum system flow 200 mL/min
Maximum collection
volume
78 mL With 30x150 mm (OD x L) tube
Maximum capacity 432 fractions
5.9 L
using 12 mm OD tubes
using 25 x 150 mm (OD x L) tubes
Trigger modes Off Fraction triggering is disabled. No fractions will be
collected.
Peak-based When a peak is discovered by a peak trigger, fractions
are collected. Depending on the max. fill volume, each
fraction will use one or more vessel(s).
Peak-based, collecting time slices When a peak is discovered by a peak trigger, fractions
are collected and each fraction is split into separate
slices of a specified duration. Depending on the max.
fill volume, each slice will use one or more vessel(s).
Peak-based, collecting volume
slices
When a peak is discovered by a peak trigger, fractions
are collected and each fraction is split into separate
slices of a specified volume. Depending on the max. fill
volume, each slice will use one or more vessel(s).
Peak-based with time slice
recovery
The complete output of the fraction collector is
recovered into separate slices of a specificed duration.
When a peak is discovered by a peak trigger, fractions
are collected in dedicated vessels. Depending on the
max. fill volume, Recovery and Fractions will use one or
more vessel(s).