Agilent Technologies 7100 Instruction Manual

Agilent 7100 Capillary
Electrophoresis System
in Empower
Technical Note
This technical guide describes the configuration and implementation of the G7100A
Capillary Electrophoresis in Empower 3.

Agilent 7100 CE in Empower Environment 2
Contents
Introduction 3
Set up and Configuration of CE in Empower 3 5
CE Instrument set up 5
The CE Driver 5
Various Configuration Steps for CE in Empower 5
Using the CE in Empower 12
The CE Status Dashboard in Empower – Direct Control 12
Creating an Instrument Method 15
Replenishment and Conditioning 25
Indirect detection 28
Application of external pressure 28
Empower features for CE Instrument Control 33
Known Limitations/Not tested configurations: 33
Extended CE driver features available in Agilent OpenLAB CDS
ChemStation Edition 34
Additional Information 36
Waters 36
APPENDIX - Examples 37
Example: Single test run 37
Example: Multiple injections using the IQ Kit for CE 39
Example: Indirect detection using the Plating Bath Buffer 41

Introduction
Agilent 7100 CE in Empower Environment 3
Introduction
Waters Instrument Control Software (ICS) is Waters Corporation’s
adoption of the Agilent Instrument Control Framework (ICF) for their data
systems. This guide describes how to configure and use the 7100 Capillary
Electrophoresis (CE) in Waters Empower environment.
Empower 3 offers all key functions to control the G7100A Agilent Capillary
Electrophoresis system. All established techniques are feasible, such as the
use of direct and indirect detection modes, use of special electrolyte
systems (containing micelles or gel matrices) and also capillary isoelectric
focusing (cIEF) or capillary electrochromatography (CEC). The application
of external high pressure is supported.
For data analysis, Empower offers processing tools for peak integration
and calculation of concentrations in CE specific mode.
Table 1 Supported and unsupported configurations using CE in Empower
Waters Instrument
Control Packages
Agilent ICF/
Agilent LC Driver
CE support in Waters Empower 3
ICF Support v2.2
#667005584
A.02.04
A.02.14
Supported
G7100A Capillary Electrophoresis, with the
internal components:
G7150A CE Mainframe
G7151A DAD
Min. Firmware B.06.73 (older firmware does
not support the use of RFID Tags for the DAD
UV-Lamp)
Not supported
G1600 Capillary Electrophoresis
ICF Support v2.2
#667005449
A.02.03 DU2
A.02.13
Not supported
G7100A Capillary Electrophoresis
G1600A Capillary Electrophoresis
And any lower integration

Introduction
4 Agilent 7100 CE in Empower Environment
NOTE Ensure that the Agilent LC/CE modules in the system meet or exceed the minimum
firmware requirements specified by the 3rd-party CDS software vendor and Agilent's
firmware set/firmware interoperability requirements. Agilent recommends using the latest
available firmware set.
http://www.agilent.com/en-us/firmwareDownload?whid=69761

Set up and Configuration of CE in Empower 3
Agilent 7100 CE in Empower Environment 5
Set up and Configuration of CE in Empower 3
CE Instrument set up
1Close Empower.
2Set up the CE system.
3The CE uses LAN communication, e.g. via the multiport ethernet card.
Connect the CE to the LAC/E box. If another instrument is connected
to the LAC/E, connect via switch or hub.
4Switch the instrument on.
The CE Driver
The CE driver is part of the LC Driver and not listed as a separate
component. Therefore, Agilent ICF consists of two components; the Waters
integration adds one additional component. All three components must be
present in Control Panel > Programs and Features:
Figure 1 Components in Programs and Feature
Various Configuration Steps for CE in Empower
To run CE in Empower the following items are required:
A CE specific project for data collection
The configuration of the CE instrument
Creating a CE Project
Configure a specific CE project to review the acquired data later.
1Select File > New > Project to open the New Project Wizard.
2Follow the instructions in the wizard screen and proceed with default
settings if no other values are given via Standard Operating Procedure
(SOP).

Set up and Configuration of CE in Empower 3
6 Agilent 7100 CE in Empower Environment
3In the New Project Wizard > Options window make sure to enable CE/CIA.
Figure 2 Enabled Options for the project
PreConfiguration of CE in Empower
For the following configuration options the PreConfiguration Utility is
required to access the CE configuration in the CE configuration editor:
Defining the Pressure Unit (see also chapter Known Limitations on page
33)
Defining the Temperature Control mode (Temperature Control switched on
by default)
Defining the Analog In Signal (see also chapter Known Limitations on
page 33)
NOTE Selecting PSI as pressure unit, the CE status dashboard shows the pressure in PSI while
the online plot and the resulting Aux Traces on the Chromatogram offer the values in
mbar/bar.

Set up and Configuration of CE in Empower 3
Agilent 7100 CE in Empower Environment 7
Software required ICF Support v2.2 (See chapter Additional Information on page 36 for Waters support
documentation TECN134936402).
1In the Empower Configuration Manager, select Tools > Agilent
PreConfiguration.
Figure 3 Configuration Manager
2Enter the IP address or host name of the LAC/E box that your
instrument is connected to into the pop-up screen Configuration Directory
and click Connect.
Note Do not enter the IP address of the instrument here.
Figure 4 IP address to connect

Set up and Configuration of CE in Empower 3
8 Agilent 7100 CE in Empower Environment
3Once the IP address is connected, click New to open the
PreConfiguration Utility.
Figure 5 Configuration of the CE
4In the Configuration Editor select the Agilent 7100 CE and click Auto
Configure.
5Enter the IP address of the instrument and click OK. The instrument is
detected and the CE and DAD appear on the right-hand side of the
configuration window.
Hint The default IP address is 192.168.254.11. Please refer to the G7100A User Manual if an IP
address change is required.

Set up and Configuration of CE in Empower 3
Agilent 7100 CE in Empower Environment 9
6Double-click CE G7150A on the right side of the window or select it and
click Configure at the bottom of the screen. The CE configuration
window opens.
Figure 6 Possible configuration options for CE
aSelect the preferred Pressure Unit in the CE status dashboard for use
in the data and the pressure auxiliary channel
bIf Temperature Control is not required, select Enable analysis with
temperature out of range.
NOTE Temperature control is enabled by default. The run only starts once the g
iven temperature
is reached. You can change this behavior by deselecting the box. A reboot (and
reconfiguration via the PreConfiguration Utility) is required.
cEnable Acquire for the Analog In Signal to collect data.
7Click OK to close the screen.
8Click OK to close the configuration editor.
9Click OK to close the PreConfiguration Utility.

Set up and Configuration of CE in Empower 3
10 Agilent 7100 CE in Empower Environment
Configuration of CE in Empower
Refer to the Waters Empower documentation for installation and
configuration of the LC/CE system in Empower.
1Open the Waters DHCP Server Configuration window by entering the
following command in the windows command line:
C:\Empower\Instruments\Waters DHCP Server Configuration.exe.
OR
In the Empower Configuration Manager window, select Node in the tree
on the left-hand side, then right-click on the node you want to add the
instrument to and select Properties. Select the Configure DHCP tab and
click Configure DHCP.
2Add the IP Address and MAC Address manually for the CE mainframe.
3Select Instrument Type AgilentLC and click OK to leave the screen.
Figure 7 Entering communication details for CE
4Access the Nodes Properties in the Empower Configuration Manager and
verify that the Instrument is shown as OK.

Set up and Configuration of CE in Empower 3
Agilent 7100 CE in Empower Environment 11
5Generate a new chromatographic system with the newly configured
instrument using File > New > Chromatographic System. Follow the
instructions on the screen.
Figure 8 Generating a new chromatographic system

Using the CE in Empower
12 Agilent 7100 CE in Empower Environment
Using the CE in Empower
The CE Status Dashboard in Empower – Direct Control
1Start Empower and open the Run Samples Screen.
2Select the CE/CIA specific project for data collection together with the
CE system.
3The CE Status window displays all available modules with their status
information. Details can be seen by hovering over the status bar with
the mouse.
Figure 9 CE dashboard status information
4The given space does not allow enlarging the CE status window to full
size, therefore a vertical scrollbar is present to adjust the required
section of the status dashboard.
Figure 10 CE Status dashboard – upper part

Using the CE in Empower
Agilent 7100 CE in Empower Environment 13
Figure 11 CE Status dashboard – lower part
Each component of the instrument is represented by an icon on the CE
status dashboard. Right-click an icon to access direct control. A context
menu appears and an action can be performed. Some contextual
actions require further user interaction. Click OK to trigger the action.
Figure 12 Example of direct control - actions available for bottle icon

Using the CE in Empower
14 Agilent 7100 CE in Empower Environment
Following major actions are accessible via the CE Status dashboard
only (list does not claim to be exhaustive):
Change Cassette
Switch on lamp/Switch off lamp
Set Inlet Vial
Set Replenish Vial
Unload Replenish Lifter
Unload Inlet Lifter
Set Outlet Vial
Unload Outlet Lifter
Flushing Capillary by Flush
Injection by Apply Pressure
Apply Voltage
Replenish Vials
Change Bottles
Get Vial
All actions are performed immediately and the changes are reflected in
the graphical user interface (GUI). The system is in not ready state
(yellow), while performing these actions. After the action finishes, the
instrument resumes an idle state (green). Any direct change of a
parameter in the direct control menu (control and/or method) does not
change the current instrument method.
NOTE It is not possible to see the detector signal while flushing the capillary. A feasible
workaround is to check the numerical value of the detection wavelength, which is
displayed in the DAD part of the GUI (left side).

Using the CE in Empower
Agilent 7100 CE in Empower Environment 15
Creating an Instrument Method
The instrument method contains all the parameters necessary to perform
the sample acquisition.
1Open the instrument method editor, for example via Edit > Instrument
Method.
2Click through the various tabs and enter the instrument method
parameters.
3Save the instrument method under a dedicated name. This method can
now be used as part of a method set.
The instrument method editor offers various tabs:
Figure 13 Instrument Method Editor
aInstrument Method tab: The instrument method tab provides access
to all method parameters of the CE system, one tab per module, CE
and DAD. Select the appropriate tab to enter the method
parameters. For details on the parameters refer to the online help
and the CE or DAD user manual.
bPretreatment Method tab: There are no pretreatment steps available
for CE.
cAuxiliary Channels tab: Offers selection of auxiliary traces during a
run, e.g. the CE leak current.

Using the CE in Empower
16 Agilent 7100 CE in Empower Environment
dGeneral tab: Offers option to mark this method as shutdown
method, see page 20. Allows selective shutdown of the CE and
DAD.
eInstrument Configuration tab: The CE system configuration is
available in the Instrument Configuration tab. All settings in this
dialog must be defined using the PreConfiguration Utility. Changes
in this section of the method are not applied to the system; they are
saved with the instrument method only. Any changes, such as
enabling temperature control, need to be done in the
PreConfiguration Utility to apply the new configuration to the
physical instrument. In addition, the LAC/E box has to be rebooted
and a new autoconfiguration is required in order to adjust the new
configuration with the LC Driver and Empower.

Using the CE in Empower
Agilent 7100 CE in Empower Environment 17
Instrument Method tab
The CE method Screen
The CE method screen contains all parameters used to adjust the
separation, meaning the home vials can be defined as well as Cassette
Temperature, Voltage, and Stoptime. Press F1 on the keyboard to access the
online help, offering explanations for each method parameter. The left-
hand side of the method screen includes the initial starting parameters.
Figure 14 CE method screen
NOTE Enter a Stoptime for the CE and/or the DAD. For run execution a Run Time is specified in
Empower later, ensure that both values are synchronized. For more details, see section
Executing Runs on page 21.

Using the CE in Empower
18 Agilent 7100 CE in Empower Environment
Hint Depending on the used SOP, the following best practices are used.
aEnsure that Empower Run Time and Agilent Module Stoptime
(for one module) are
synchronized.
bSet an Agilent Module Stoptime of 1 min, and set the real sample time in the
sample set screen.
If an entered parameter is out of range, a warning sign is shown. By
hovering with the mouse pointer over the parameter, the message displays
the possible range, e.g. Voltage is out of range [-30kV to 30 kV].
Figure 15 Out of range handling
The right-hand side of the method screen offers additional features. The
online help explains each of the features:
Replenishment
Preconditioning
Injection
Timetable
Postconditioning

Using the CE in Empower
Agilent 7100 CE in Empower Environment 19
The DAD method screen
On the left side, the DAD method offers the main method parameters to
acquire up to eight signals. Refer to the online help to learn more about
each parameter. Remember to set the stop time either to CE or to the same
time as the Empower run time.
The right side offers additional method parameters, e.g. for spectra
collection and a timetable, in order to change parameters during a run.
Unlike with other Agilent DAD, there is no VIS-lamp available.
Figure 16 DAD method screen

Using the CE in Empower
20 Agilent 7100 CE in Empower Environment
General Method Tab
Empower offers the option of selecting a System Shutdown Method, which is
executed at the end of the sequence.
1To generate a shutdown method, define the desired shutdown
conditions, such as vials and times in the CE/DAD method screen first
and select Shutdown after Run. It is possible to perform a selective
shutdown, for example only the DAD.
2To execute the shutdown method, either select the shutdown method in
the Run Sample set window or load the shutdown method as the last
line of the sequence.
Figure 17 Shutdown Method screen