Labnet BioPette Multichannel User manual

MULTICHANNEL
Labnet International, Inc.
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MULTICHANNEL
AA1
A2
C
D
E
B
F
H
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MULTICHANNEL
G
4A 4B 4C 4D
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1 2
MULTICHANNEL
The specifications for accuracy and precision
given in the follo ing table are obtained using
Labnet tips. These figures are only guaranteed
hen
Labnet tips are used.
BioPette 8
channel
BioPette 12 channel
These specifications are obtained in for ard
mode, using a gravimetric method ith the tem-
perature of the distilled ater, tips and all other
conditions stabilized bet een 19°C and 21°C.
The values given include all components of
error due to both normal hand arming and
changing of the tips.
Performance tests: The pipette is calibrated in
accordance ith EN ISO 8655. Performance
can be verified by checking the pipette using
CONTENTS
1 - INTRODUCTION
2 - VOLUME SETTING
3 - METHOD OF PIPETTING
4 - RECOMMENDATIONS
5 - RECALIBRATION
6 - CLEANING AND STERYLIZATION
7 - PIPETTE KIT AND ACCESSORIES
8 - SPARE PARTS
1 - INTRODUCTION
The BioPette™ series of adjustable multichannel
pipettes have been designed for the filling of la-
boratory microplates. The pipettes enable precise
and simultaneous delivery of 8 or 12 preset-vol-
ume doses of liquid.
BioPette™ pipettes are
produced in four ranges of volumes: 1-10 µl,
5-50 µl, 20-200 µl, 50-300 µl.
The BioPette™ pipettes are equipped ith a di-
gital counter hich sho s the pipetting volume.
The set volume is visible in the indo on the
handle. The setting of the volume is done by tur -
ning of the pipetting pushbutton knob (Fig. 1A2)
or by turning of the black knurled adjustment
knob (Fig. 1B) in the right direction. The volume
range is sho n on the pipetting pushbutton
(Fig. 1A1).
BioPette™ pipettes should be used ith poly-
propylene disposable tips, to ensure safety and
accuracy, (Fig. 1H). The shafts ithout O-rings
ensure the compatibility of the pipettes ith a
broad assortment of tips, and the special ejector
shape reduces the force required for their ejec-
tion. In order to protect the user against inad-
vertent contact ith used tips, the pipettes are
equipped ith tip ejectors, (Fig. 1F). By turning
the multichannel module clock ise in relation
to the handle, (Fig. 2), it is possible to disable
the ejector’s operation in order to avoid acci-
dental use, or to change its position in such a
ay as to make filling of microplates more con-
venient.
Min 1 ±8.0 ± 6.0
BPE-10 5 ±4.0 ± 2.0
Max 10 ±2.0 ± 1.2
Min 5 ±4.0 ± 2.5
BPE-50 25 ±3.0 ± 1.2
Max 50 ±1.6 ± 0.6
Min 20 ±3.0 ± 1.5
BPE-200 100 ±1.5 ± 0.8
Max 200 ±1.0 ± 0.6
Min 50 ±1.6 ± 1.5
BPE-300 150 ±1.2 ± 1.0
Max 300 ±1.0 ± 0.6
Model Volume Accuracy Precision
[µl] [%] [%]
SPECIFICATIONS
Min 1 ±8.0 ± 6.0
BPT-10 5 ±4.0 ± 2.0
Max 10 ±2.0 ± 1.2
Min 5 ±4.0 ± 2.5
BPT-50 25 ±3.0 ± 1.2
Max 50 ±1.6 ± 0.6
Min 20 ±3.0 ± 1.5
BPT-200 100 ±1.5 ± 0.8
Max 200 ±1.0 ± 0.6
Min 50 ±1.6 ± 1.5
BPT-300 150 ±1.2 ± 1.0
Max 300 ±1.0 ± 0.6
Model Volume Accuracy Precision
[µl] [%] [%]
SPECIFICATIONS
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3 4
MULTICHANNEL
If the knob is accidentally turned too far, ie is
set lo er than the required value, the process
must be repeated. The approach to the set vol-
ume must al ays be made in the order of
decreasing value.
- METHOD OF PIPETTING
Fit the tips onto cones of the multichannel module.
In order to put tips on the multichannel shafts
hold the pipette vertically and press it against
the tips in the rack box, until the shafts
retreat
about 1.5 mm into the multichannel module.
The
suspension system of the shafts ensures even
and tight sealing of tips. The rolling movement
does not have to be performed to seal the tips
tightly. The liquid aspirated into the tips should
not flo out by gravity from properly fixed tips.
While holding the pipette in a vertical position,
tips should be immersed in the liquid to a depth
of 2-4 mm, and flushed once by dra ing a dose
of liquid and dispensing it out ith slo and
steady movement.
Next, still holding the pipette vertically, the ope-
rator should press the pipetting button until the
first resistance point is felt, and immerse the tips
in the liquid to the depth of 2-4 mm, (Fig. 3B).
Releasing the pipetting button ith a slo and
uniform movement during 2-3 seconds, the liquid
should be dra n into the tips, (Fig. 3B) hich
should then be lifted above the liquid’s surface.
The pipette should next be positioned at an
angle of 10-45 degrees in relation to inner alls
of destination vessels and tips should
be emptied by pressing the pipetting button
slo ly until the first resistance point is felt,
(Fig. 3C). After aiting one second, the pipetting
button should be pressed to the second resis-
tance point in order to expel remaining liquid,
(Fig. 3D). Then tips should be lifted out from the
vessels hile maintaining contact bet een
the ends of the tips and the inner alls of the
vessel until the pipetting button has been rela-
sed, (Fig. 3E). Finally, pressing the ejector but-
ton, the operator should separate the tips from
multichannel module’s cones, (Fig. 3F).
the procedures outlined in the EN ISO 8655
standard.
The pipette design enables the user to perform
the recalibration process according to the rules
presented in section 5.
2 - SETTING THE VOLUME
Volume is set using the pipetting pushbutton
(Fig. 1A2) or
black adjustment knob (Fig. 1B),
hile the value is sho n
on the digital counter.
The volume sho n by the counter is represented
by three digits hich should be read from top to
bottom. Typical readings are sho n in the fol-
lo ing table:
To attain the maximum accuracy, set volume
must be approached from a higher value. If the
desired value is lo er than the previous value,
simply use the knob in the pushbutton (Fig. 1A2)
or the black adjustment knob (Fig. 1B) to adjust
the value do n to 1/3 turn above the required set-
ting. If the desired value is higher than the pre-
vious value, use the knob in the pushbutton or the
black adjustment ring to adjust the value to 1/3
turn above the required value. The setting should
then be performed in the follo ing manner:
With the volume set to 1/3 above the required
setting, slo ly turn the pipetting pushbutton
knob or the black adjustment knob to the
required volume.
Model
Counter
readings
Set
volume Increments
0
3
5
2
5
0
0
8
5
0
6
5
BPE-10
BPT-10
BPE-50
BPT-50
BPE-200
BPT-200
BPE-300
BPT-300
3.5 µl
6.5 µl
85.0 µl
250.0 µl
0.02 µl
0.10 µl
0.20 µl
1.0 µl
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MULTICHANNEL
6
to check hether the follo ing requirements
have been fulfilled during error determination:
• the ambient temperature, and the temperature
of the pipette, tips and ater as identical
• the density of the liquid used is close to that
of distilled ater
•
a balance ith appropriate sensitivity has been
used
• mg/µl conversion factor has been taken into
account
•the requirements given in sections 3 and 4
have been fulfilled
If the above conditions are satisfied and the accu-
racy error for selected volume given in section 1
exceeds the permissible value, the pipette
recalibration procedure should be carried out.
The recalibration can be performed within one
full turn of the key to the right or to the left only.
Recalibration conditions:
• Ambient temperature and the temperature of
the pipette, tips and liquid should be ithin
the range 20-25°C and stabilized during eigh-
ing
ithin ±0.5 ° C
•Measurements should be conducted using
distilled ater
• Balance sensitivity should be suitable for the
volume to be controlled
Recalibration procedure:
• Set the dose volume depending on the pipette
volume according to the follo ing table:
5
4 - RECOMMENDATIONS
To achieve maximum safety, precision and re-
liability, the follo ing principles should be ob-
served:
• do not dra liquids ithout tips fitted on the
pipette cones,
• do not lay do n the pipette ith tips filled,
• do not dra volumes of liquid exceeding the
pipette’s range,
• check if the tips are fitted properly,
• during operation, the pipette must be held ver-
tically, tips should be immersed in liquid
to the depth of 2 to 4 milimeters and the pipet-
ting button should be depressed and released
slo ly and evenly,
• ne tips must be pre-rinsed prior to pipetting,
by dra ing and expelling the liquid to be me-
asured. This is especially important hen or-
king ith liquids of viscosities and densities
different from that of ater and in temperatu-
res different from ambient temperature,
• hen pipeting liquids hich tend to et alls
of tips, such as serums, proteins or organic
solvents, measuring must be performed much
slo er than ith other liquids,
•
tips must be replaced ith ne ones, hen
changing from one liquid to another, or if drops
of liquid remain inside the tips,
• after ork, the pipette should be stored verti-
cally in a holder ith tips removed.
5 - RECALIBRATION
BioPette™ pipettes are calibrated by gravimetric
method, using
Labnet tips and distilled ater, at
the temperature 20±1°C, according to EN ISO
8655 standard.
If during pipette operation you find that the
accuracy error (the difference bet een the real
aspirated volume and the preset volume)
exceeds the permissible value given in the table
in section 1, the pipette recalibration procedure
should be carried out.
Before starting the recalibration it is necessary
Volume checked [µl]
0.1 - 10
10 - 100
> 100
Balance sensitivity [mg]
≤ 0.001
≤ 0.01
≤ 0.1
1 - 10
5 - 50
20 - 200
50 - 300
1
5
20
50
0.92 - 1.08
4.8 - 5.2
19.6 - 20.4
49.2 - 50.8
0.33
1.67
6.30
10.00
Range
of the pipette
volumes [µl]
Preset
volume
[µl]
Permissible
volumes
[µl]
Volume change ∆V for
full turn of the calibra-
tion key [µl]
(24 increments
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7 8
MULTICHANNEL
•Perform three aspiration series (each series
should include the aspirations from all chan-
nels), eigh each time and calculate the aver-
age value of the aspirations.
• Calculate average aspirated volume in µl mul-
tiplying the average aspiration amount [mg]
by the distilled ater density coefficient
[µl/mg], hich depends on temperature and
pressure according to the follo ing table:
If the average aspirated volume exceeds the
permissible value, the follo ing should be done:
• Remove the pipetting pushbutton, (Fig. 4A)
Warning: The pipetting pushbutton consists
of 2 parts: the knob (Fig. 1A2) and the
pushbutton (Fig. 1A1). After removal of the
pushbutton, both parts are separated.
•Holding the volume setting knob to protect
it against rotation, insert the calibration key
into the cuts of the calibration scre , (Fig. 4B)
• Turn the key clock ise to reduce the aspirat-
ed volume, or counter-clock ise to increase
the volume, (Fig. 4C). One full turn of the calibra-
tion key changes the pipette aspiration volume
by the amount given in the table
• Take out the key and fix the pipetting pushbut-
ton (Fig. 4D). The pipetting pushbutton should
be fixed by placing the knob on the arbor first
(Fig. 1A2) and then the pushbutton (Fig. 1A1).
Determine the average aspirated volume. The
average volume should be ithin the permissi-
ble range given in the table. If the volume
exceeds the values stated, the recalibration pro-
cedure should be repeated.
6 - CLEANING AND STERILIZATION
Cleaning
External surfaces of the pipetting pushbutton,
the ejector button, the handgrip, shaft and the
adjustment knob may be cleaned using a cloth
dampened in isopropyl alcohol.
Sterilization:
The pipette can be sterilized in the autoclave at
121 °C for 20 minutes. After sterilization, the pi-
pette should be dried and cooled to room tem-
perature.
It is recommended:
- to sterilize the pipette in autoclave ith an initial
vacuum and drying cycle,
- prior to sterilization unscre the nut connecting
the handle and module slightly. After autoclav-
ing these parts should be scre ed tight again.
The precision of the results should not alter if the
pipetting process and autoclaving are carried out
as described in this manual. Because a slight
change in the accuracy of the dosage may
occur, it is recommended to:
- check the calibration of the pipette after the ini-
tial first, third and fifth autoclaving cycles and
then after every 10 autoclaving cycles.
7 - PIPETTE KIT AND ACCESSORIES
Pipette kit
The pipettes are delivered in the kits including:
• Pipette
• Instruction manual
• Calibration key
• Identification labels
Temperature [°C] Pressure [kPa]
95 101.3 105
20 1.0028 1.0029 1.0029
21 1.0030 1.0031 1.0031
22 1.0032 1.0033 1.0033
23 1.0034 1.0035 1.0036
24 1.0037 1.0038 1.0038
25 1.0039 1.0040 1.0040
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910
MULTICHANNEL
Accessories
8 - SPARE PARTS
The spare parts for multichannel, (Fig. 1, 4):
A: Pipetting pushbutton
A1: Pushbutton A2: Knob
B: Adjustment knob
C: Ejector button
D: Piston’s assembl
E: Shaft
F: Ejector
G: Calibration ke
Can be ordered from a Labnet representative
(type of the pipette and name of the part for this
pipette should be specified).
Before returning a pipette to a Labnet representa-
tive please ensure that the pipette is completely
free of any contamination (chemical, microbial
or radioactive).
Contact Labnet at 732-417-0700,
fax: [phone],
email: [email protected]
Warning: The replacement of the plunger
requires conducting of calibration proce-
dure according to section 5.
BPM/08/2004
Pipettor Stand Choices
P 5421 Stand for eight channel pipette
BPE
P 5422 Stand for t elve channel pipette
BPT
Pipettor Tip Choices
P 4803-10, 10 µl tips, racked
BPE-10
P 4803-200, 200 µl tips, racked
BPE-50
BPE-200
BPT-50
BPT-200
P 4803-300, 300 µl tips, racked
BPE-300
BPT-300
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