Beckman Coulter Optima MAX-XP User manual

393552AH
August 2018
Instructions For Use
Optima MAX-XP
Ultracentrifuge
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Optima MAX-XP
Ultracentrifuge
393552AH (August 2018)
© 2018 Beckman Coulter, Inc.
Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Contact Us
If you have any questions, contact our Customer Support
Center.
• Worldwide, find us via our website at
www.beckman.com/support/technical.
• In the USA and Canada, call us at 1-800-369-0333.
• Outside of the USA and Canada, contact your local
Beckman Coulter Representative.
Find us on the World Wide Web at:
www.beckman.com
Beckman Coulter Eurocenter S.A.
22, rue Juste-Olivier
Case Postale 1044
CH - 1260 Nyon 1, Switzerland
Tel: [phone]
Glossary of Symbols is available at
beckman.com/techdocs (PN C24689).
Original Instructions

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Revision History
Your Biomek i-Series documentation can be found on our website. For updates, go to
www.beckman.com/techdocs and download the latest version of the manual or system help for your
instrument.
Revision AG, 04/2017 - Changes or additions were made to:Multiple Compliance.
Revision AH, 08/2018 - Changes or additions were made to: CHAPTER 4, User Message Chart;
CHAPTER 5, Supplies.
Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin of the
amended page.

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Revision History

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Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate the ultracentrifuge. Do not attempt to perform any procedure before carefully reading
all instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt
as to how to proceed in any situation, contact your Beckman Coulter representative.
Alerts for Danger, Warning, Caution, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury.
NOTE
NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety During Installation and/or Maintenance
This ultracentrifuge is designed to be installed by a Beckman Coulter Field Service representative.
Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty
covering the ultracentrifuge.
This ultracentrifuge weighs 105 kg (230 lb). Do not attempt to lift or move it without assistance.
Any servicing of this equipment that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is off and the
ultracentrifuge is disconnected from the main power source, and refer such servicing to qualified
personnel.
Do not replace any ultracentrifuge components with parts not specified for use on this
ultracentrifuge.

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vi
Safety Notice
Electrical Safety
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to
connect the ultracentrifuge to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to
the ultracentrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding a type of multiple-outlet
receptacle strip.
• Do not install the ultracentrifuge on a ground fault-protected power source.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the ultracentrifuge and damage electrical or mechanical components.
Safety Against Risk of Fire
Fuses protect certain electrical circuits within this ultracentrifuge against overcurrent conditions.
For continued protection against the risk of fire, replace only with the same type and rating
specified.
This ultracentrifuge is not designed for use with materials capable of developing flammable or
explosive vapors. Do not ultracentrifuge such materials (such as chloroform or ethyl alcohol) in this
ultracentrifuge nor handle or store them within the required 30-cm (1-ft) area surrounding the
ultracentrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the rotors and accessories designed for use in this ultracentrifuge.
• Do not exceed the maximum rated speed of the rotor in use.
• Do not lift or move the ultracentrifuge while the rotor is spinning.
• NEVER attempt to slow or stop the rotor by hand.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• In the event of a power failure, do not attempt to retrieve the sample from the ultracentrifuge
for at least 1 hour. Then follow the instructions for sample recovery in
CHAPTER 4, Troubleshooting.

393552AH vii
Safety Notice
Chemical and Biological Safety
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this ultracentrifuge, however, unless all necessary
safety precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to
their use.
• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) and HIV (I-V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this ultracentrifuge without taking appropriate safety precautions.
Biosafe containment should be used when Risk Group II materials (as identified in the World
Health Organization Laboratory Biosafety Manual are handled; materials of a higher group
require more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the ultracentrifuge and accessories before requesting
service by Beckman Coulter Field Service.
Summary of Instrument Labels
This section provides information for some labels and symbols appearing on the Optima MAX-XP
instrument housing. These labels and symbols may be associated with user-serviceable procedures.
Individual hazards associated with a specific procedure in this manual may use these labels and
symbols, and are included in Warnings or Cautions within the procedures for that task.
Caution Symbol
This symbol indicates a caution message and appears adjacent to an explanation or other symbols
that define the caution.
!

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Safety Notice
Summary of Instrument Labels
RoHS Caution
This label and materials declaration table (the Table of Hazardous Substance's Name and
Concentration) are to meet People's Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
This logo indicates that this electronic information product contains certain toxic or hazardous
elements, and can be used safely during its environmental protection use period. The number in the
middle of the logo indicates the environmental protection use period for the product. The outer
circle indicates that the product can be recycled. The logo also signifies that the product should be
recycled immediately after its environmental protection use period has expired. The date on the
label indicates the date of manufacture.
Recycling Label
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
• the device was put on the European Market after August 13, 2005 and
• the device is not to be disposed of via the municipal waste collection system of any member
state of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label, please contact your dealer or local Beckman Coulter office for details on the take-back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
A28219-AA

393552AH ix
Safety Notice
Summary of Instrument Labels
Multiple Compliance
• Recycling – Refer to the Recycling Label section in this document.
• – A “CE” mark indicates that a product has been assessed before being placed on the
market, and has been found to meet European Union safety, health, and/or environmental
protection requirements.
• 169502 – This label indicates recognition by a Nationally Recognized Testing Laboratory (NRTL)
that the instrument has met the relevant product safety standards.
NOTE
169502 is applicable to North American models only.
• The RCM mark is intended for use on products that comply with Australian Communications
Media Authority (ACMA) EMC Requirements.
CAUTION Static Sensitive Area
Indicates an area of the instrument which is sensitive to static electrostatic discharge (ESD). To
prevent damage due to electrostatic discharge, always wear a properly earth-grounded wrist strap
while operating this instrument. For details on proper grounding, see IEEE standard P1100.
DANGER High Voltage
Operation, replacement or servicing of any components where contact with bare, live hazardous
parts could occur, possibly resulting in electric shock, should only be performed by your
Beckman Coulter representative.

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Safety Notice
Summary of Instrument Labels
Protective Ground
This symbol is used to indicate a protective ground. This instrument must be properly grounded.
Do not under any circumstances operate the instrument unless it is properly grounded.
WARNING Risk of Fire
Before replacing fuses, shut off power and disconnect the power cord. Failure to do so can cause
electric shock and/or equipment damage. Replace fuses only with approved type and rating
replacement fuse.
Universal Serial Bus (USB)
This symbol indicates the location of a universal serial bus (USB) connector.
MAX-XP CAPACITY
This label indicates oil capacities for the indicated subsystems.

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Safety Notice
Summary of Instrument Labels
WARNING 240 VAC
This symbol indicates that the instrument has been configured for operation at 240 VAC. Consult
with a Beckman Coulter service engineer for use at other line voltages.
NOTICE Regarding Vacuum Pump Oil
This label indicates to use only ULVAC R2 rotary vacuum pump oil (Beckman Coulter part number
A51210).

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Safety Notice
Summary of Instrument Labels
Rotor Rotation
This indicates the direction of instrument rotor rotation.
SER NO (Serial Number)
SER NO
This label indicates the location of the instrument serial number.

xiii
Contents
Revision History,iii
Safety Notice,v
Alerts for Danger, Warning, Caution, and Note,v
Safety During Installation and/or Maintenance,v
Electrical Safety,vi
Safety Against Risk of Fire,vi
Mechanical Safety,vi
Chemical and Biological Safety,vii
Summary of Instrument Labels,vii
Introduction,xix
Certification,xix
Scope of Manual,xix
Typographic Conventions,xx
CFC-Free Centrifugation,xx
Radio Interference,xx
CHAPTER 1: Description,1-1
Intended Use,1-1
Rotor Chamber,1-2
Vacuum System,1-2
Temperature Sensing and Control,1-2
Drive,1-3
Controls and Indicators,1-3
Power Switch,1-3
Touchscreen Interface,1-3
Safety Features,1-6
Door,1-6
Barrier Ring,1-6
Imbalance Detector,1-6
Overspeed and Rotor Identification System,1-7
Name Rating Plate,1-7
Specifications,1-7

xiv
Contents
Control Features,1-7
Physical Data,1-8
Available Rotors,1-9
CHAPTER 2: Introduction,2-1
Space Requirements,2-1
Electrical Requirements,2-2
CHAPTER 3: Introduction,3-1
Touchscreen Interface,3-1
Modes of Operation,3-2
Summary of Optima MAX-XP Run Procedures,3-3
Ultracentrifuge and Rotor Preparation,3-3
Manual Run,3-4
Programmed Run,3-5
Manual Operation,3-6
Preparing the Ultracentrifuge,3-6
Logging In,3-6
Selecting a Rotor,3-7
Entering Run Speed,3-8
Entering Run Time,3-9
Entering Run Temperature,3-15
Entering Acceleration and Deceleration Rates,3-16
Starting a Run,3-19
Stopping a Run,3-20
Viewing a Previous Run,3-21
Changing the Login Button Design,3-22
Programmed Operation,3-23
Creating a New Program,3-24
Starting a Programmed Run,3-31
Making Changes to a Program,3-34
Deleting a Program,3-37
Pulse Run Operation,3-38
Remote Operation,3-40
Administrator Operations,3-40
Accessing Administrator Options,3-40
Set Interface Language,3-41
Setting the Date and Time,3-43
Adding and Managing Users,3-44
Setting User Login,3-49
Setting Rotor Logging,3-50
Populating the Rotor Library,3-50
Setting Audio Volume,3-54
Exporting Data,3-54

xv
Contents
CHAPTER 4: Introduction,4-1
User Messages,4-1
Retrieving Your Sample in Case of Power Failure,4-6
Circuit Breaker,4-9
CHAPTER 5: Ultracentrifuge Care,5-1
Vacuum System,5-1
Drive Hub,5-2
Air-Intake and Exhaust Louvers,5-2
Cleaning,5-2
Ultracentrifuge Surfaces,5-2
Touchscreen Display,5-2
Decontamination,5-3
Sterilization and Disinfection,5-3
Storage and Transportation,5-3
Supply List,5-3
Supplies,5-4
Optional Accessories,5-4
Warranty for the Optima MAX-XP Ultracentrifuge

xvi
Illustrations
Illustrations
1.1 The Optima MAX-XP Ultracentrifuge,1-1
1.2 Temperature Control Diagram,1-3
1.3 Touchscreen Interface,1-4
2.1 Dimensions of the Optima MAX-XP Ultracentrifuge,2-2
3.1 Touchscreen Interface Main Screen,3-2
4.1 Example User Message on Touchscreen Interface,4-2
4.2 Removing the Top Cover of the Ultracentrifuge,4-7
4.3 Door Lock System,4-8

xvii
Tables
Tables
3.1 Acceleration and Deceleration Rates,3-17
4.1 User Message Chart,4-2

xviii
Tables

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Introduction
Certification
To ensure full system quality, the Beckman Coulter Optima MAX-XP Ultracentrifuge has been
manufactured in a registered ISO 9001 or 13485 facility. It has been designed and tested to be
compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements
of applicable regulatory agencies. Declarations of conformity and certificates of compliance are
available at www.beckmancoulter.com.
Scope of Manual
This manual is designed to acquaint you with the Optima MAX-XP Ultracentrifuge, its functions,
specifications, operation, and routine operator care and maintenance. We recommend that you
read this entire manual, especially the Safety Notice and all safety-related information, before
operating the ultracentrifuge or performing ultracentrifuge maintenance.
• CHAPTER 1 contains system specifications and a brief physical and functional description of the
ultracentrifuge, including the operating controls and indicators.
• CHAPTER 2 describes the space and power requirements for installing and connecting the
ultracentrifuge.
• CHAPTER 3 describes the ultracentrifuge operating procedures.
• CHAPTER 4 lists possible malfunctions with possible causes and suggested corrective actions.
• CHAPTER 5 contains procedures for routine operator care and maintenance, as well as a brief
list of supplies, replacement parts, and accessories.
CAUTION
If the ultracentrifuge is used in a manner other than that specified in this manual,
the safety and performance of this equipment could be impaired. Further, the use
of any equipment other than that recommended by Beckman Coulter has not been
evaluated for safety. Use of any equipment not specifically recommended in this
manual and/or the appropriate rotor manual is the sole responsibility of the user.

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Introduction
Typographic Conventions
Typographic Conventions
Certain symbols are used in this manual to call out safety-related and other important information.
These international symbols may also be displayed on the ultracentrifuge and are reproduced on
the inside back cover of this manual.
Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as buttons and displays.
• Touchscreen buttons (for example,
START or VACUUM ) appear in boldface.
• Display names (for example,
SPEED RPM or TIME hr:mm appear in boldface.
CFC-Free Centrifugation
To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the
Optima MAX-XP Ultracentrifuge.
Radio Interference
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of FCC rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used
in accordance with this instruction manual, may cause interference to radio communications.
Operation of this equipment in a residential area is likely to cause interference, in which case the
user will be required to correct the interference at his own expense.
Canadian Regulations
This equipment does not exceed the Class A limits for radio noise emissions from digital apparatus
as set out in the radio interference regulations of the Canadian Department of Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites
applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage
radioelectrique édicté par le Ministère des Communications du Canada.