Beckman Coulter SW 32 Ti User manual

LXL-TB-015BB
November 2011
Instructions For Use
SW 32 Ti and SW 32.1 Ti
Swinging-Bucket Rotors
For Use in Beckman Coulter
Class H, R, and S
Preparative Ultracentrifuges
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821

SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
LXL-TB-015AE (November 2011)
Copyright © 2011 Beckman Coulter, Inc.
All rights reserved. No part of this document may be
reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.
Find us on the World Wide Web at:
www.beckmancoulter.com
Beckman Coulter Ireland, Inc.
Mervue Business Park, Mervue Galway, Ireland 353 91 774068
製造販売元 : ベックマン･コールター株式会社
東京都江東区有明三丁目5番7号
TOC 有明ウエストタワー
贝克曼库尔特有限公司，
美国加利福尼亚州，Brea 市，S. Kraemer 大街 250 号，
邮编：92821 电话：[phone]

LXL-TB-015BB iii
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to use this equipment. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
This safety notice summarizes information basic to the safe use of the rotors described in this
manual. The international symbol displayed to the left is a reminder to the user that all safety
instructions should be read and understood before operation or maintenance of this equipment is
attempted. When you see the symbol on other pages of this publication, pay special attention to the
safety information presented. Observance of safety precautions will also help to avoid actions
that could damage or adversely affect the performance of the rotor. These rotors were developed,
manufactured, and tested for safety and reliability as part of a Beckman Coulter ultracentrifuge/
rotor system. Their safety or reliability cannot be assured if used in an ultracentrifuge not of
Beckman Coulter’s manufacture or in a Beckman Coulter ultracentrifuge that has been modified
without Beckman Coulter’s approval.
Alerts for Danger, Warning, Caution, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety Information for the SW 32 Ti and SW 32.1 Ti Rotors
Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that such fluids are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) viruses, HIV (I–V), atypical mycobacteria, and certain systemic fungi—further emphasize the
need for aerosol protection. Handle other infectious samples according to good laboratory
!

LXL-TB-015BB
iv
Safety Notice
Safety Information for the SW 32 Ti and SW 32.1 Ti Rotors
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in these rotors without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require more
than one level of protection.
The rotors and accessories are not designed for use with materials capable of developing flammable
or explosive vapors. Do not centrifuge such materials in nor handle or store them near the
centrifuge
Although rotor components and accessories made by other manufacturers may fit in the SW 32 Ti
and SW 32.1 Ti rotors, their safety in these rotors cannot be ascertained by Beckman Coulter. Use of
other manufacturers’ components or accessories in these rotors may void the rotor warranty and
should be prohibited by your laboratory safety officer. Only the components and accessories listed
in this publication should be used in this rotor.
Attach all six buckets, loaded or empty, to the rotor for every run. Make sure that filled containers
are loaded symmetrically into the rotor and that opposing tubes are filled to the same level with
liquid of the same density. Make sure that buckets containing Quick-Seal tubes have the proper
floating spacers inserted (if applicable) before installing the bucket cap.
If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor.
Apply appropriate decontamination procedures to the centrifuge and accessories.
Never exceed the maximum rated speed of the rotor and labware in use. Refer to the section on Run
Speeds, and derate the run speed as appropriate.
Do not use sharp tools on the rotor that could cause scratches in the rotor surface. Corrosion begins
in scratches and may open fissures in the rotor with continued use.

v
Contents
Safety Notice,iii
Alerts for Danger, Warning, Caution, and Note, iii
Safety Information for the SW 32 Ti and SW 32.1 Ti Rotors,iii
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors,1
Specifications for the SW 32 Ti Rotor,1
Specifications for the SW 32.1 Ti Rotor,2
Description,3
Preparation and Use,4
Prerun Safety Checks,4
Rotor Preparation,4
Operation,6
Removal and Sample Recovery,7
Tubes and Accessories,7
Run Times,12
Run Speeds,13
Selecting CsCl Gradients,19
Adjusting Fill Volumes,19
Typical Examples for Determining CsCl Run Parameters,20
Care and Maintenance,21
Maintenance,21
Cleaning,22
Decontamination,23
Sterilization and Disinfection,23
Storage,23
Returning a Rotor,23
Supply List,24
Replacement Rotor Parts,24
Other,25
Warranty

vi

vii
Illustrations
Illustrations
1 Arranging Tubes Symmetrically in the Rotor Buckets,6
2 Precipitation Curves for the SW 32 Ti Rotor.r,15
3 CsCl Gradients at Equilibrium for the SW 32 Ti Rotor,16
4 Precipitation Curves for the SW 32.1 Ti Rotor,17
5 CsCl Gradients at Equilibrium for the SW 32.1 Ti ,18

viii
Tables
Tables
1 Beckman Coulter Tubes and Accessories for the SW 32 Ti
Rotor,8
2 Beckman Coulter Tubes and Accessories for the SW 32.1 Ti
Rotor,9
3 Relative Centrifugal Fields for the SW 32 Ti and SW 32.1 Ti
Rotors,14

LXL-TB-015BB 1
SW 32 Ti and SW 32.1 Ti
Swinging-Bucket Rotors
Specifications for the SW 32 Ti Rotor
1. Axis of Rotation
Maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32,000 RPM
Density rating at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL
Relative Centrifugal Fieldaat maximum speed
At rmax (152.5 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175,000 g
At rav (109.7 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126,000 g
At rmin (66.8 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76,600 g
kfactor at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 204
kfactor at maximum speed (5 to 20% sucrose gradient; 5°C)
When particle density = 1.3 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 468
When particle density = 1.5 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 428
When particle density = 1.7 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 412
Conditions requiring speed reductions . . . . . . . . . . . . . . . . . . . . see Run Speeds
Number of buckets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Available tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 1
Nominal tube dimensions (largest tube) . . . . . . . . . . . . . . . . . . . . . 25 89 mm
Nominal tube capacity (largest tube) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38.5 mL
Nominal rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 231 mL
Approximate acceleration time to maximum speed
(rotor fully loaded). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 3/4min
Approximate deceleration time from maximum speed
(rotor fully loaded . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1/2min
Weight of fully loaded rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.5 kg (18.7 lb)
Rotor and bucket material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . titanium
a. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rw2) to the standard acceleration of
gravity (g) according to the following formula: RCF = r/g — where ris the radius in millimeters, is the angular velocity in radians per
second (2 RPM /60), and gis the standard acceleration of gravity (9807 mm/s2). After substitution: RCF = 1.12r (RPM/1000)2
rmin
rav
rmax
1

LXL-TB-015BB
2
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Specifications for the SW 32.1 Ti Rotor
Specifications for the SW 32.1 Ti Rotor
1. Axis of Rotation
Maximum speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32,000 RPM
Density rating at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1.2 g/mL
Relative Centrifugal Fieldaat maximum speed
At rmax (162.8 mm). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 187,000 g
At rav (113.6 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130,000 g
At rmin (64.4 mm) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73,900 g
kfactor at maximum speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 229
kfactor at maximum speed (5 to 20% sucrose gradient; 5°C)
When particle density = 1.3 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 613
When particle density = 1.5 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 560
When particle density = 1.7 g/mL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 536
Conditions requiring speed reductions . . . . . . . . . . . . . . . . . . . . see Run Speeds
Number of buckets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Available tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . see Table 2
Nominal tube dimensions (largest tube) . . . . . . . . . . . . . . . . . . . . 16 102 mm
Nominal tube capacity (largest tube) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 mL
Nominal rotor capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102 mL
Approximate acceleration time to maximum speed
(rotor fully loaded). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 1/2min
Approximate deceleration time from maximum speed
(rotor fully loaded . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 1/4min
Weight of fully loaded rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8.3 kg (18.3 lb)
Rotor and bucket material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . titanium
a. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rw2) to the standard acceleration of
gravity (g) according to the following formula: RCF = r/g — where ris the radius in millimeters, is the angular velocity in radians per
second (2 RPM /60), and gis the standard acceleration of gravity (9807 mm/s2). After substitution: RCF = 1.12r (RPM/1000)2
r
min
r
av
r
max
1

LXL-TB-015BB 3
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Description
Description
These Beckman Coulter rotors have been manufactured in an ISO 9001 or 13485 facility for use with the
specified Beckman Coulter ultracentrifuges.
The SW 32 Ti and SW 32.1 Ti are swinging bucket rotors designed to centrifuge up to six tubes each.
Used in Beckman Coulter class H, R, and S preparative ultracentrifuges, these rotors develop
centrifugal forces for the separation of subcellular particles and viruses in density gradients. The
rotors have a common rotor body with buckets that can be used interchangeably (see Rotor
Preparation). Bucket and rotor body positions are numbered for operator convenience.
The rotor body and buckets are made of titanium, finished with polyurethane paint. Bucket caps are
made of aluminum, anodized for corrosion resistance. Each bucket and cap assembly sets into
grooves in the rotor body. O-rings, made of Buna N rubber, between each bucket and bucket cap
maintain atmospheric pressure inside the buckets during centrifugation.
For overspeed protection, a Beckman Coulter ultracentrifuge equipped with a photoelectric
detector will monitor the overspeed disk on the rotor bottom and shut down the run if a speed
exceeding the maximum allowable run speed is detected.
See the Warranty at the back of this manual for warranty information.
1. Black Cap
2. O-ring (812715)
3. Bucket (SW 32 Ti)
4. Rotor
5. Overspeed Disk (335456)
1
5
4
3
2

LXL-TB-015BB
4
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
Preparation and Use
Specific information about the SW 32 Ti and SW 32.1 Ti rotors is given here. Information common to these
and other rotors is contained in Rotors and Tubes for Preparative Ultracentrifuges (publication LR-IM),
which should be used together with this manual for complete rotor and accessory operation. Publication
LR-IM is included in the literature package with this rotor manual.
NOTE Although rotor components and accessories made by other manufacturers may fit in the SW 32 Ti and
SW 32.1 Ti rotors, their safety in these rotors cannot be ascertained by Beckman Coulter. Use of other
manufacturers’ components or accessories in these rotors may void the rotor warranty and should be
prohibited by your laboratory safety officer. Only the components and accessories listed in this
publication should be used in these rotors.
Prerun Safety Checks
Read the Safety Notice section at the front of this manual before using the rotor.
1 Make sure that the rotor, buckets, and caps are clean and show no signs of corrosion or
cracking.
2 Make sure that the rotor is equipped with the correct overspeed disk.
a. If the disk is missing or damaged, replace it according to the instructions in Rotors
and Tubes.
3 Verify that the tubes and bottles being used are listed in Table 1 or Table 2.
4 Check the chemical compatibilities of all materials used.
• Refer to Chemical Resistances (publication IN-175), included in the Rotors and Tubes CD.
Rotor Preparation
For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration.
NOTE Place the rotor on the rotor stand (332400) when it is not in the centrifuge. Take care to protect the
overspeed disk from damage when handling the rotor.
32,000-RPM
56-Sector
(335456)

LXL-TB-015BB 5
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
1 Install the rotor body on the drive hub.
a. Slowly turn the rotor to the right (clockwise) to make sure it is properly seated.
2 Load the filled containers into the buckets (see page 7 for tube and accessory information).
a. Complete loading by placing the correct floating spacers (if required) over the tubes.
3 Ensure that bucket gaskets are lightly but evenly coated with silicone vacuum grease.
a. Do not run a bucket without an O-ring, as the bucket will leak.
CAUTION
Use only black caps (369643) on black SW 32 Ti buckets and red caps (369645) on
red SW 32.1 Ti buckets.
4 Use a lint-free cotton swab to apply Spinkote™ lubricant (396812) to cap grooves in the
bucket tops.
a. Match bucket caps with numbered buckets.
b. Align the pins on each side of the cap with the guide slots in the bucket.
c. Twist the cap clockwise until it stops (one-quarter turn).
1. Ta b
2. Black Cap
3. O-ring (812715)
4. SW 32 Ti Bucket
5. Ta b
6. Red Cap
7. O-ring (812715)
8. SW 32.1 Ti Bucket
1. Cap Pins
2. Ta b
3. Bucket Top View
4. Guide Slots
5. This side toward outside of rotor
15
4
3
26
7
8
1
4
3
2
5

LXL-TB-015BB
6
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
5 Grasp the bucket cap tabs and lower the buckets into the openings on the rotor body with the
rounded sides toward the outside of the rotor.
NOTE A bucket will not seat in the rotor if it is inserted in the rotor with the rounded side toward the rotor
center. The bucket will fall through, spilling or disturbing the tube contents.
Six bucket assemblies must be installed, whether loaded or empty. If fewer than six tubes are being run,
they must be arranged symmetrically in the rotor (see Figure 1). Opposing tubes must be filled to
the same level with liquid of the same density.
Figure 1 Arranging Tubes Symmetrically in the Rotor Buckets
NOTE Two, three, four, or six tubes can be centrifuged per run if they are arranged in the rotor as shown. All
bucket assemblies must be attached to the rotor, whether loaded or empty.
Operation
For low-temperature runs, precool the rotor in the centrifuge or in a refrigerator before use—especially
before short runs—to ensure that the rotor reaches the set temperature. A suggested precooling cycle is a
minimum of 30 minutes at 2000 RPM at the required temperature.
1 Refer to the centrifuge instruction manual for additional operating instructions.
2 For additional operating information, see the following:
• Run Times, page 12, for using kfactors to adjust run durations.
• Run Speeds, page 13, for information about speed limitations.
• Selecting CsCl Gradients, page 19, for methods to avoid CsCl precipitation during
centrifugation.

LXL-TB-015BB 7
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
Removal and Sample Recovery
CAUTION
If disassembly reveals evidence of leakage, you should assume that some fluid
escaped the rotor. Apply appropriate decontamination procedures to the
centrifuge and accessories.
1 Grasp the bucket cap tabs and lift up to remove the buckets.
• (Buckets may be removed without removing the rotor body from the ultracentrifuge.)
2 Remove the bucket caps by twisting counterclockwise and lifting up.
a. Use a tube removal tool (361668) or hemostat to remove the spacers and tubes.
NOTE If the conical-shaped adapters that support konical tubes are difficult to remove after
centrifugation, an extractor tool (354468) is available to facilitate removal.
3 To remove the rotor from the centrifuge, lift the rotor straight up and off the drive hub.
a. Set the rotor on the rotor stand.
Tubes and Accessories
The SW 32 Ti rotor uses tubes and accessories listed in Table 1; the SW 32.1 Ti rotor uses tubes and
accessories listed in Table 2. Be sure to use only those items listed, and to observe the maximum
speed limits shown. Refer to Appendix A in Rotors and Tubes for information on the chemical
resistances of tube and accessory materials.
1. Extractor Tool (354468)
While pressing the rubber tip against
the adapter wall, pull the tube and
adapter up and out of the cavity.
1

LXL-TB-015BB
8
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
Table 1 Beckman Coulter Tubes and Accessories for the SW 32 Ti Rotora
Tube Required Accessory
Max Speed/
RCF/
kfactor
Dimensions/
Nominal
Volume/ Description
Part
Number Description
Part
Number
25 89 mm
38.5 mL
Ultra Clear
open-top
344058
(pkg/50)
none — 32,000 RPM
175,000 g
204
25 89 mm
38.5 mL
polyallomer
open-top
326823
(pkg/50)
none — 32,000 RPM
175,000 g
204
25 89 mm
31 mL
thickwall
polyallomer
open-top
355642
(pkg/25)
none — 32,000 RPM
175,000 g
198
25 89 mm
31 mL
thickwall
polycarbonate
open-top
355631
(pkg/25)
none — 32,000 RPM
175,000 g
202
25 83 mm
33.5 mL
polyallmer
Quick-Seal, bell-top
344623
(pkg/50)
Norylbfloating
spacer
355536 32,000 RPM
175,000 g
192
25 89 mm
31.5 mL
konicalpolyallomer
open-top
358126
(pkg/50)
Hytrelcadapter 358156
(pkg/6)
32,000 RPM
175,000 g
199
25 83 mm
28 mL
konicalpolyallomer
Quick-Seal,bell-top
358651
(pkg/50)
adapter 358156 32,000 RPM
175,000 g
198
Noryl floating spacer 355536
25 76 mm
25.5 mL
konical polyallomer
open-top
358125
(pkg/50)
Hytrel adapter 358156
(pkg/6)
32,000 RPM
175,000 g
156
25 64 mm
27 mL
polyallomer
Quick-Seal, bell-top
343665
(pkg/50)
Noryl floating spacer 355536 32,000 RPM
175,000 g
146
25 77 mm
32.4 mL
OptiSeal bell-topd361625
(pkg/50)
Ultem spacerb392833 32,000 RPM
175,000 g
155
25 38 mm
15 mL
polyallomer
Quick-Seal,
bell-top
343664
(pkg/50)
Noryl floating spacer 355536 32,000 RPM
175,000 g
81

LXL-TB-015BB 9
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
25 38 mm
8.4 mL
konicalpolyallomer
Quick-Seal, bell-top
358652
(pkg/50)
Hytrel adapter 358156
(pkg/6)
32,000 RPM
175,000 g
74
Noryl floating spacer 355536
25 76 mm
22.5 mL
konical polyallomer
Quick-Seal, bell-top
358654
(pkg/50)
Hytrel adapter 358156 32,000 RPM
175,000 g
155
Noryl floating spacer 355536
a. Use only the items listed here..
b. Noryl and Ultem are registered trademarks of GE Plastics.
c. Hytrel is a registered trademark of E.I. Du Pont de Nemours and Company.
d. Includes disposable plastic plugs.
Table 2 Beckman Coulter Tubes and Accessories for the SW 32.1 Ti Rotora
Tube Required Accessory
Max Speed/
RCF/
kfactor
Dimensions/
Nominal
Volume/ Description
Part
Number Description
Part
Number
16 102 mm
14.5 mL
polyallomer
Quick-Seal,
bell-top
356291
(pkg/50)
Noryl floating spacer 355579 32,000 RPM
187,000 g
199
16 102 mm
17 mL
Ultra-Clear,
open-top
344061
(pkg/50)
none — 32,000 RPM
187,000 g
228
16 102 mm
12.5 mL
konicalpolyallomer
Quick-Seal bell-top
358653
(pkg/50)
adapter 358155 32,000 RPM
187,000 g
205
Noryl floating spacer 355579
16 96 mm
16.5 mL
polyallomer,
open-top
337986
(pkg/50)
none — 32,000 RPM
187,000 g
228
16 93 mm
13.5 mL
konicalpolyallomer
open-top
358123
(pkg/50)
Hytrel adapter 358155 32,000 RPM
187,000 g
225
Table 1 Beckman Coulter Tubes and Accessories for the SW 32 Ti Rotora(Continued)
Tube Required Accessory
Max Speed/
RCF/
kfactor
Dimensions/
Nominal
Volume/ Description
Part
Number Description
Part
Number

LXL-TB-015BB
10
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
Temperature Limits
• Plastic tubes have been centrifuge tested for use at temperatures between 4 and 25°C. For
centrifugation at other temperatures, pretest tubes under anticipated run conditions.
• If plastic containers are frozen before use, make sure that they are thawed to at least 4°C prior
to centrifugation.
OptiSeal Tubes
OptiSeal™ tubes come with plastic plugs and can be quickly and easily prepared for use. With the
tube spacer in place, the gforce during centrifugation ensures a tight, reliable seal that protects
your samples.
16 67 mm
10.5 mL
polyallomer
Quick-Seal, bell-top
344622
(pkg/50)
Noryl floating spacer 355579 32,000 RPM
187,000 g
133
16 57 mm
8 mL
polyallomer
Quick-Seal, bell-top
344621
(pkg/50)
Noryl floating spacer 355579 32,000 RPM
187,000 g
102
16 44 mm
6.5 mL
polyallomer
Quick-Seal,
bell-top
345830
(pkg/50)
Noryl floating spacer 355579 32,000 RPM
187,000 g
79
16 32 mm
4.5 mL
polyallomer
Quick-Seal,
bell-top
356562
(pkg/50)
Noryl floating spacer 355579 32,000 RPM
187,000 g
56
a. Use only the items listed here..
Table 2 Beckman Coulter Tubes and Accessories for the SW 32.1 Ti Rotora(Continued)
Tube Required Accessory
Max Speed/
RCF/
kfactor
Dimensions/
Nominal
Volume/ Description
Part
Number Description
Part
Number
4°C

LXL-TB-015BB 11
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
1 Place the tubes in the rack and fill each tube to the base of the stem, leaving no fluid in the stem.
• Overfilling the tube can cause spillage when the plug is inserted or can compromise seal
integrity.
• However, too much air can cause excessive tube deformation, disrupting gradients and
sample bands.
2 Refer to Using OptiSeal™ Tubes (publication IN-189), included in each box of tubes, for detailed
information on the use and care of OptiSeal tubes.
Quick Seal Tubes
Quick-Seal® tubes must be sealed prior to centrifugation. These tubes are heat sealed and do not
need caps; however, spacers are required on top of the tubes when they are loaded into the rotor
buckets.
1 Fill Quick-Seal tubes leaving a small bubble of air at the base of the neck.
a. Do not leave a large air space—too much air can cause excessive tube deformation.
2 Some of the tubes listed in Table 1 and Table 2 are part of the g-Max™ system, which uses a
combination of small bell-top Quick-Seal tubes and floating spacers (also called g-Max spacers).
• This means that you can run the shorter tubes listed in the Tables in the SW 32 Ti and
SW 32.1 T rotors without reduction in gforce.
• For detailed information on the g-Max system see publication DS-709.
1. Spacer
2. Plug
3. Stem
4. Meniscus
5. Tu b e
6. Base of Stem
1. g-Max Spacer
2. Bell-top Tube
1
5
4
3
2
6
1
2

LXL-TB-015BB
12
SW 32 Ti and SW 32.1 Ti Swinging-Bucket Rotors
Preparation and Use
3 Refer to Rotors and Tubes for detailed information on the use and care of Quick-Seal tubes.
• Quick-Seal tubes are disposable and should be discarded after a single use.
konical™ Tubes
Polyallomer konical tubes, used to optimize pelleting separations, have a conical tip that
concentrates the pellet in the narrow end of the tube. The narrow bottom also reduces the tube’s
nominal volume and minimizes gradient material requirement. The konical tubes come in both
open-top and Quick-Seal tube designs. Conical cavity adapters hold the tubes in the rotor buckets
Polyallomer and Ultra-Clear Open-Top Tubes
Polyallomer and Ultra-Clear® open-top tubes should be filled as full as possible (2 or 3 mm from the
tube top) for tube support. If necessary, float mineral oil (or some other low-density, immiscible
liquid) on top of the tube contents to fill the tube to its maximum volume. (Do not use an oil overlay
in Ultra-Clear tubes.) All opposing tubes for a run must be filled to the same level with liquid of the
same density.
Run Times
The kfactor of the rotor is a measure of the rotor’s pelleting efficiency. (Beckman Coulter
has calculated the kfactors for all of its preparative rotors at maximum rated speed and using
full tubes.) The kfactor is calculated from the formula
EQ 1
where is the angular velocity of the rotor in radians per second (= 0.105 RPM), rmax is the
maximum radius, and rmin is the minimum radius.
After substitution:
EQ 2
1. Adapters
1
krmax rmin
ln

2
-----------------------------------1013
3600
------------
=
k2.533 1011
rmax rmin ln
RPM2
------------------------------------------------------------------------=