Beckman Coulter Avanti J-26S XPI User manual

PN B10093AE
April 2019
Instructions For Use
Avanti J-26S XPI
High-Performance Centrifuge
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Avanti J-26S XPI
High-Performance Centrifuge
PN B10093AE (April 2019)
© 2019 Beckman Coulter, Inc.
All rights reserved.
Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Contact Us
If you have any questions, contact our Customer Support
Center.
• Worldwide, find us via our website at
www.beckman.com/support/technical.
• In the USA and Canada, call us at 1-800-369-0333.
• Outside of the USA and Canada, contact your local
Beckman Coulter Representative.
Find us on the World Wide Web at: www.beckman.com
Beckman Coulter Eurocenter S.A.
22, rue Juste-Olivier
Case Postale 1044
CH - 1260 Nyon 1, Switzerland
Tel: [phone]
Glossary of Symbols is available at
beckman.com/techdocs (PN C24689).
Original Instructions

PN B10093AE iii
Revision History
For labeling updates, go to www.beckman.com/techdocs and download the latest version of the manual or
system help for your instrument.
Issue AD, 05/2016
Updates were made to the following sections: Multi Compliance Label; CHAPTER 1, Control Features
Issue AE, 04/2019
Updates were made to the following sections: CHAPTER 1, Control Features
Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin of the
amended page.

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Revision History

PN B10093AE v
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate the instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Danger, Warning, Caution, Important, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety During Installation and/or Maintenance
This instrument is designed to be installed by a Beckman Coulter Field Service representative.
Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty
covering the instrument. Also, if the instrument needs to be moved, a Beckman Coulter Field
Service representative must reinstall and relevel the instrument in its new location.
Any servicing of this equipment that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is turned off and the
instrument is disconnected from the main power source by removing the Mains (power) plug from
the outlet receptacle, and refer such servicing to qualified personnel.

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Safety Notice
Electrical Safety
Be sure to use the anchoring system to secure the centrifuge in place. The anchoring system is
designed to reduce the possibility of injury or damage that could result from instrument movement
in the event of a major rotor mishap.
Do not replace any centrifuge components with parts not specified for use on this instrument.
Electrical Safety
To reduce the risk of electrical shock, this instrument uses a three or five-wire electrical cord and
plug to connect this equipment to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to
the centrifuge.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the instrument and damage electrical or mechanical components.
Safety Against Risk of Fire
This instrument is not designed for use with materials capable of developing flammable or
explosive vapors. Do not centrifuge such materials (for example, chloroform or ethyl alcohol) in
this instrument nor handle or store them near the centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this instrument.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not lift or move the centrifuge while a rotor is spinning.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Do not lean on the centrifuge or place items on it while it is operating.

PN B10093AE vii
Safety Notice
Chemical and Biological Safety
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this instrument unless all necessary safety
precautions are taken.
• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the instrument and accessories before requesting service
by Beckman Coulter Field Service.
RoHS Notice
These labels and materials declaration table (the Table of Hazardous Substance’s Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
China RoHS Caution Label
This label indicates that the electronic information product contains certain toxic or hazardous
substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and
indicates the number of calendar years the product can be in operation. Upon the expiration of the
EFUP, the product must be immediately recycled. The circling arrows indicate the product is
recyclable. The date code on the label or product indicates the date of manufacture.
China RoHS Environmental Label
This label indicates that the electronic information product does not contain any toxic or hazardous
substances. The center “e” indicates the product is environmentally safe and does not have an

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Safety Notice
RoHS Notice
Environmentally Friendly Use Period (EFUP) date. Therefore, it can safely be used indefinitely. The
circling arrows indicate the product is recyclable. The date code on the label or product indicates
the date of manufacture.

ix
Contents
Revision History,iii
Safety Notice,v
Alerts for Danger, Warning, Caution, Important, and Note,v
Safety During Installation and/or Maintenance,v
Electrical Safety,vi
Safety Against Risk of Fire,vi
Mechanical Safety,vi
Chemical and Biological Safety,vii
RoHS Notice,vii
China RoHS Caution Label,vii
China RoHS Environmental Label,vii
Introduction,xvii
Certification,xvii
Scope of Manual,xvii
Conventions,xvii
Typographic Conventions,xviii
CFC-Free Centrifugation,xviii
Recycling Label,xviii
Multi Compliance Label,xix
CHAPTER 1: Description,1-1
Introduction,1-1
Centrifuge Function and Safety Features,1-1
Centrifuge Function,1-1
Safety Features,1-2
Centrifuge Chassis,1-2
Housing and Door,1-2
Rotor Chamber,1-3
Drive,1-3
Friction Reduction System (FRS),1-3
Temperature Sensing and Control,1-3
Overtemp System,1-3
Name Rating Plate,1-4

x
Contents
Controls and Indicators,1-4
Key and Power Switches,1-4
Control Panel,1-5
Actual Value Display,1-5
Setup Screen,1-6
Function Keys,1-6
Keypad,1-8
System Keys,1-8
Specifications,1-8
Control Features,1-9
Operational Features,1-9
Physical Data,1-10
Available Rotors,1-11
CHAPTER 2: Operation,2-1
Introduction,2-1
Summary of Avanti J-26S XPI Run Procedures,2-1
Manual Run,2-1
Programmed Run,2-2
Preparation,2-3
Installing the Rotor,2-3
Manual Operation,2-4
Selecting a Rotor,2-4
Entering Run Speed,2-5
Entering RPM,2-5
Entering RCF,2-6
Entering Run Time,2-7
Entering a Timed Run,2-7
Entering a w2t Run,2-8
Entering a Continuous (HOLD) Run,2-9
Entering Run Temperature,2-10
Entering Acceleration and Deceleration Rates,2-11
Acceleration,2-11
Deceleration,2-12
Starting a Run,2-12
Changing Parameters During a Run,2-13
Ending a Run,2-13
Programmed Operation,2-13
Creating a New Program,2-13
Modifying a Program,2-15
Running a Program,2-16
Setting Up a Zonal Run,2-16
Setting Up an Elutriation Run,2-18

xi
Contents
CHAPTER 3: Troubleshooting,3-1
Introduction,3-1
User Messages,3-1
Accessing the Rotor in Case of Power Failure,3-4
JCF-Z Rotor Identification,3-9
CHAPTER 4: Care and Maintenance,4-1
Introduction,4-1
Maintenance,4-1
Cleaning,4-2
Tube Breakage,4-2
Decontamination,4-3
Sterilization and Disinfection,4-3
Replacing the Air Filter,4-3
Circuit Breaker and Fuses,4-4
Storage and Transport,4-5
Storage,4-5
Returning a Centrifuge,4-5
Supply List,4-5
Replacement Parts and Supplies,4-6
APPENDIX A: Preinstallation Requirements,A-1
Preinstallation Requirements,A-1
Electrical Requirements,A-1
Single and Three-Phase Power Connections,A-2
Additional Requirements for Three-Phase Power
Connections,A-4
Space and Location Requirements,A-4
Securing the Centrifuge to the Floor,A-5
Bio-Safety Level 3 Installation,A-5
Non-Invasive Centrifuge Restraint System for Vinyl Floors,A-5
Using J2 Series Rotors in the Avanti J-26S XPI,A-7
Checking for Rotor Drive Pins,A-7
Using the JA-18 Rotor,A-8
Using the JCF-Z Continuous Flow/Zonal Rotor,A-8
Zonal Bracket Kits,A-8
Ensuring Correct JCF-Z Continuous Flow/Zonal Rotor
Identification,A-8
APPENDIX B: Temperature Calibration Procedure,B-1
Introduction,B-1
Beckman Coulter, Inc.

xii
Contents
Avanti J Series Centrifuge Warranty
Related Documents

xiii
Illustrations
Illustrations
1.1 The Power Switch,1-4
1.2 The Control Panel,1-5
1.3 The Actual Value Display,1-5
1.4 The Setup Screen,1-6
2.1 Elutriation Controls,2-18
3.1 Emergency Door Release Latch Access,3-5
3.2 Manual Door Release,3-6
3.3 Venting the Chamber Vacuum,3-7
3.4 Front Panel Retaining Strip,3-8
A.1 Single-Phase Electrical Requirements,A-3
A.2 Three-Phase “Y” Electrical Requirements,A-3
A.3 Correct and Incorrect Three-Phase Power Configurations,A-4
A.4 Rear View and Dimensions,A-6
A.5 Checking the Rotor for Drive Pins,A-7

xiv
Illustrations

xv
Tables
Tables
2.1 Acceleration and Deceleration Settings,2-11
3.1 Diagnostic Message Chart,3-2
A.1 Nominal Supply Voltage Ratings for the Avanti J-26S XPI,A-2
A.2 Required Wire Connections,A-3

xvi
Tables

PN B10093AE xvii
Introduction
Certification
Beckman Coulter Avanti J-26S XPI centrifuges are manufactured in a facility that maintains
certifications to both ISO 9001:2008 and ISO 13485:2003. They have been designed and tested to be
compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements
of applicable regulatory agencies. Declarations of conformity and certificates of compliance are
available at www.beckman.com.
Scope of Manual
This manual is designed to familiarize centrifuge users and site engineers with the Avanti J-26S XPI
centrifuge, its functions, specifications, operation, and routine care and maintenance.
Beckman Coulter recommends that you read this entire manual, especially the Safety Notice and
all safety-related information, before operating the instrument or performing instrument
maintenance.
• CHAPTER 1, Description contains an overall description of the centrifuge, including a
description of system controls and indicators and system specifications.
• CHAPTER 2, Operation summarizes procedures for operating the centrifuge.
• CHAPTER 3, Troubleshooting lists system diagnostic messages, together with probable causes
and user-performed corrective actions.
• CHAPTER 4, Care and Maintenance contains procedures for routine maintenance, as well as a
brief list of supplies and replacement parts.
• APPENDIX A, Preinstallation Requirements contains instructions for preparing your site for
centrifuge installation.
• APPENDIX B, Temperature Calibration Procedure contains a procedure to use when temperature
control within 1°C is required.
NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual is the sole responsibility of the user.
Conventions
Certain symbols are used in the product labeling call out safety related and other important
information. These international symbols may also be displayed on the centrifuge and are
reproduced on the inside back cover of the manual.

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Introduction
CFC-Free Centrifugation
Typographic Conventions
Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.
• Control knob names (for example, ROTOR and SPEED) appear in bold type.
• Key names (for example, START and STOP) appear in bold type.
• Display field names (for example, TEMP°C or SPEED) appear in bold type.
CFC-Free Centrifugation
To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the
Avanti J-26S XPI centrifuge.
Recycling Label
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
1. The device was put on the European market after August 13, 2005 and
2. The device is not to be disposed via the municipal waste collection system of any member state
of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
A28219-AA

PN B10093AE xix
Introduction
Multi Compliance Label
Multi Compliance Label
• The RCM mark is intended for use on products that comply with Austrailian Communications
Media Authority (ACMA) EMC Requirements.
• Recycling — Refer to the Recycling Label section in this document.
• 169502 — This label indicates recognition by a Nationally Recognized Testing Laboratory
(NRTL) that the instrument has met the relevant product safety standards.
NOTE 169502 is applicable to North American models only.
• — A “CE” mark indicates that a product has been assessed before being placed on the
market, and has been found to meet European Union safety, health, and/or environmental
protection requirements.

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Introduction
Multi Compliance Label