Beckman Coulter Optima XPN User manual

PN B08115AF
February 2018
User Guide
Optima XPN Series
Preparative Ultracentrifuges
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Optima XPN
Ultra Centrifuge
User Guide
PN B08115AF (February 2018)
© 2018 Beckman Coulter, Inc.
All rights reserved
Trademarks
Beckman Coulter, the stylized logo, and the Beckman
Coulter product and service marks mentioned herein are
trademarks or registered trademarks of Beckman Coulter,
Inc. in the United States and other countries.
All other trademarks, service marks, products, or services
are trademarks or registered trademarks of their
respective holders.
Contact Us
If you have any questions, contact our Customer Support
Center.
• Worldwide, find us via our website at
www.beckmancoulter.com/customersupport/support.
• In the USA and Canada, call us at 1-800-369-0333.
• Outside of the USA and Canada, contact your local
Beckman Coulter Representative.
Printed in U.S.A.

PN B08115AF iii
Revision History
B08115AA, Initial Issue, 09/2011
Issue Version AB, 01/2014
Manual updated per Canadian Standards Association (CSA) guidelines.
Issue Version AC, 01/2015
Changes or additions were made to: Getting Access to the Sample; Restoring the Instrument to
Operating Condition.
Issue Version AD, 04/2016
Changes or additions were made to: Multiple Compliance
Issue Version AE, 09/2016
Changes or additions were made to:
• Chemical and Biological Safety
• CHAPTER 2, System References
• CHAPTER 8, References Page
Issue Version AF, 02/2018
Changes or additions were made to: Table C.1, Diagnostics/User Messages Chart.
Note: Changes that are part of the most recent revision are indicated in text by a bar in the margin
of the amended page.
This document applies to the latest software listed and higher versions. When a subsequent software version affects the
information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to
www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.

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Revision History

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Safety and Notices
This chapter presents the important notices that apply to the instrument and describes the
conventions used in the document.
Alerts for Danger, Warning, Caution, Important, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury. This signal word is to be limited to the most
extreme situations.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury. May be used to indicate the possibility of
erroneous data that could result in an incorrect diagnosis (does not apply to all
products).
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices. May be used to indicate the possibility of erroneous data that could
result in an incorrect diagnosis (does not apply to all products).
IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the Important adds benefit to the performance of a piece of equipment or to a
process.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.

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Safety and Notices
Safety Notices
Safety Notices
Before installing, using, or maintaining the instrument, be certain that you know all the following
precautions.
Information
CAUTION
Read all product manuals and consult with Beckman Coulter-trained personnel
before attempting to operate instrument. Do not attempt to perform any
procedure before carefully reading all instructions. Always follow product labeling
and manufacturer’s recommendations. If in doubt as to how to proceed in any
situation, contact your Beckman Coulter Representative.
Installation
CAUTION
This instrument is designed to be installed by a Beckman Coulter Field Service
representative. Installation by anyone other than authorized Beckman Coulter
personnel invalidates any warranty covering the instrument. Also, should the
instrument need to be moved, a Beckman Coulter Field Service representative
must reinstall and re-level the instrument in its new location.
WARNING
Do not place the ultracentrifuge near areas containing flammable or combustible
fluids, or any other source of vapors that could enter the ultracentrifuge air
system and be ignited by the motor.
WARNING
Ultracentrifuge operations generate high energy levels that require precautions
against sudden movements that could result from the rare event of a rotor failure.
Maintain a 30-cm. (1-ft.) clearance envelope around and above the
ultracentrifuge. Do not install any equipment or furniture in this envelope. While
the ultracentrifuge is running, keep the envelope clear of any persons or objects
and do not reach into the envelope except when required to change operating
controls.

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Safety and Notices
Safety Notices
Replacement Parts
WARNING
Do not replace any centrifuge components with parts not specified for use on this
instrument.
Service
WARNING
Any servicing of this equipment that requires removal of any covers can expose
parts which involve the risk of electric shock or personal injury. Make sure that the
power switch is turned off, and the instrument is disconnected from the main
power source, by removing its power plug from the receptacle. Refer such
servicing to qualified personnel.
NOTE It is your responsibility to decontaminate the instrument and accessories before
requesting service by Beckman Coulter Field Service.
Power Supply
DANGER
To reduce the risk of electrical shock, this instrument uses a three-wire electrical
cord and plug to connect this equipment to earth-ground. Make sure that the
matching wall outlet receptacle is properly wired and earth-grounded.
Fuse Replacement
WARNING
Fuses protect certain electrical circuits within this instrument against overcurrent
conditions. The fuse is not customer replaceable. For continued protections,

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Safety and Notices
Safety Notices
please contact Beckman Coulter Field Service.
Mechanical Safety
DANGER
For safe operation of the equipment, observe the following:
• Use only the Beckman Coulter rotors and accessories designed for use in this
instrument.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop a rotor by hand.
• Do not move the centrifuge while the drive motor is spinning.
• In the event of a power failure, do not attempt to retrieve the sample from the
instrument for at least one hour. Then follow the instructions for recovery of
the sample in the Maintenance and Troubleshooting chapter.
Chemical and Biological Safety
DANGER
Normal operation may involve the use of solutions and test samples that are
pathogenic, toxic, or radioactive. Such materials require that you take all
necessary safety precautions.
• Handle body fluids with care because they can transmit disease. No known
test offers complete assurance that they are free of micro-organisms.
• Handle all infectious samples according to good laboratory procedures and
methods to prevent spread of disease.
• Because spills may generate aerosols, observe proper safety precautions for
aerosol containment.
• Some of the most virulent infectious agents—Hepatitis (B and C) and HIV (I-V)
viruses, atypical mycobacteria, and certain systemic fungi—require
additional emphasis on aerosol protection.
• Do not run toxic, pathogenic, or radioactive materials in a rotor without taking
appropriate safety precautions.
• Risk Group II materials (as identified in the World Health Organization
Laboratory Biosafety Manual) require biosafe containment. Materials of a
higher group require more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health
and safety guidelines.
• Do not centrifuge flammable or explosive vapors, or materials capable of
hazardous chemical reactions.

PN B08115AF ix
Safety and Notices
Certification
WARNING
California Proposition 65:
This product may contain chemicals known to the State of California to cause
cancer and birth defects or other reproductive harm.
Precautions with Liquids
WARNING
Do not place containers holding liquid on or near the chamber door. Liquid, if
spilled, may get into the instrument and damage electrical or mechanical
components.
Volatile Liquids
DANGER
This instrument is not designed for use with materials capable of developing
flammable or explosive vapors, or hazardous chemical reactions. Do not
centrifuge such materials (for example, chloroform or ethyl alcohol) in this
instrument nor handle or store them near the centrifuge.
Certification
To ensure full system quality, Beckman Coulter Optima XPNs have been manufactured in a
registered ISO 9001 or 13485 facility. They have been designed and tested to conform to (when used
with Beckman Coulter rotors) the laboratory equipment requirements of applicable regulatory
agencies. Declarations of conformity and certificates of compliance are available at
www.beckmancoulter.com.
Scope of Manual
This manual is designed to familiarize you with the Optima XPN, its functions, specifications,
operation, and routine operator care and maintenance. Read this entire manual, especially the
safety notices and all safety-related information, before operating the instrument or performing
maintenance.
NOTE If the instrument is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that recommended
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual and/or the appropriate rotor manual is the sole responsibility of the user.

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Safety and Notices
CFC-Free Centrifugation
CFC-Free Centrifugation
To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the
Optima XPN ultracentrifuge.
Software Copyright
The software and other information incorporated into the Optima XPN is protected by international
copyright laws. Unauthorized copying, use, distribution, transfer, or sale is a violation of those laws
that may result in civil or criminal penalties. This computer program is also subject to additional
restrictions contained in the following Microsoft OEM Customer License Agreement for Embedded
Systems:
“If you use the Device to access or utilize the services or functionality of Microsoft Windows XP
Server (all editions), or use the Device to permit workstation or computing devices to access or
utilize the services or functionality of Microsoft Windows XP Server, you may be required to obtain
a Client Access License for the Device and/or each such workstation or computing device. Refer to
the End-User License Agreement for Microsoft Windows XP Server for additional information.” The
End-User License Agreement is available on the Microsoft Embedded Systems website.

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Safety and Notices
RoHS Notice
RoHS Notice
These labels and materials declaration table (the Table of Hazardous Substance's Name and
Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006
“Marking for Control of Pollution Caused by Electronic Information Products” requirements.
China RoHS Caution Label
This label indicates that the electronic information product contains certain toxic or hazardous
substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and
indicates the number of calendar years the product can be in operation. Upon the expiration of the
EFUP, the product must be immediately recycled. The circling arrows indicate the product is
recyclable. The date code on the label or product indicates the date of manufacture.
China RoHS Environmental Label
This label indicates that the electronic information product does not contain any toxic or hazardous
substances. The center “e” indicates the product is environmentally safe and does not have an
Environmentally Friendly Use Period (EFUP) date. Therefore, it can safely be used indefinitely. The
circling arrows indicate the product is recyclable. The date code on the label or product indicates
the date of manufacture.

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Safety and Notices
Summary of Instrument Labels
Summary of Instrument Labels
This section provides information for some labels and symbols appearing on the Optima XE
instrument housing. These labels and symbols may be associated with user-serviceable procedures.
Individual hazards associated with a specific procedure in this manual may use these labels and
symbols, and are included in Warnings or Cautions within the procedures for that task.
Caution Symbol
This symbol indicates a caution message and appears adjacent to an explanation or other symbols
that define the caution.
Universal Serial Bus (USB)
This symbol indicates the location of a universal serial bus (USB) connector.
Ethernet
This symbol indicates the location of an ethernet connector.
!

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Safety and Notices
Summary of Instrument Labels
Recycling Label
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
• the device was put on the European Market after August 13, 2005 and
• the device is not to be disposed of via the municipal waste collection system of any member
state of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label, please contact your dealer or local Beckman Coulter office for details on the take-back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
Multiple Compliance
This symbol indicates compliance with:
• 169502 – This label indicates recognition by a Nationally Recognized Testing Laboratory (NRTL)
that the instrument has met the relevant product safety standards.
• The RCM mark is intended for use on products that comply with Australian communications
Media Authority (ACMA) EMC Requirements.
• Recycling – Refer to the Recycling Label section in this document.
• – A “CE” mark indicates that a product has been assessed before being placed on the
market, and has been found to meet European Union safety, health, and/or environmental
protection requirements.
A28219-AA

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Safety and Notices
Summary of Instrument Labels
CAUTION Static Sensitive Area
Indicates an area of the instrument which is sensitive to static electrostatic discharge (ESD). To
prevent damage due to electrostatic discharge, always wear a properly earth-grounded wrist strap
while operating this instrument. For details on proper grounding, see IEEE standard P1100.
DANGER High Voltage
Operation, replacement or servicing of any components where contact with bare, live hazardous
parts could occur, possibly resulting in electric shock, should only be performed by your
Beckman Coulter representative.
Protective Ground
This symbol is used to indicate a protective ground. This instrument must be properly grounded.
Do not under any circumstances operate the instrument unless it is properly grounded.
Alternating Current
This symbol is used to indicate an alternating current (also known as “AC”).

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Safety and Notices
Summary of Instrument Labels
Output Plate Label
This is the label that indicates what the output rating are on the instrument.
Rotor Rotation
This indicates the direction of instrument rotor rotation.

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Safety and Notices
Symbols
Symbols

xvii
Contents
Revision History,iii
Safety and Notices,v
Alerts for Danger, Warning, Caution, Important, and Note,v
Safety Notices,vi
Information,vi
Installation,vi
Replacement Parts,vii
Service,vii
Power Supply,vii
Fuse Replacement,vii
Mechanical Safety, viii
Chemical and Biological Safety,viii
Precautions with Liquids,ix
Volatile Liquids,ix
Certification,ix
Scope of Manual,ix
CFC-Free Centrifugation,x
Software Copyright,x
RoHS Notice,xi
China RoHS Caution Label,xi
China RoHS Environmental Label,xi
Summary of Instrument Labels,xii
Caution Symbol,xii
Universal Serial Bus (USB),xii
Ethernet,xii
Recycling Label,xiii
Multiple Compliance,xiii
CAUTION Static Sensitive Area,xiv
DANGER High Voltage,xiv
Protective Ground,xiv
Alternating Current,xiv
Output Plate Label,xv
Rotor Rotation,xv
Symbols,xvi
CHAPTER 1: Description,1-1
Touch Screen,1-1

xviii
Contents
Name Rating Plate,1-1
Rotor Chamber,1-1
Vacuum System,1-1
Temperature Sensing and Control,1-2
Drive,1-2
Safety Features,1-2
Door,1-2
Barrier Ring,1-3
Imbalance Detector,1-3
Overspeed System,1-3
Dynamic Rotor Inertia Check (DRIC),1-3
Specifications,1-3
Control Features,1-4
Physical Data,1-6
Audible Sounds,1-7
Available Rotors,1-8
CHAPTER 2: The Touch Screen Interface,2-1
Areas on the Screen,2-1
Header Bar,2-1
Home Page Button,2-2
Menu Button,2-2
Status Display,2-2
Help Button,2-3
Footer Bar,2-3
Start Button,2-4
Stop Button,2-4
Footer Bar on the Home Page,2-5
Footer Bar on Other Pages,2-5
Vacuum Display/Button,2-5
System Name,2-5
Accel and Decel Display/Button,2-6
Page Display Area,2-6
Help Messages,2-6
Item Help,2-6
Global Help,2-7
System References,2-8
About Your Instrument,2-9
CHAPTER 3: Operations,3-1
Manual Operation,3-1
Step 1: Start on the Home Page,3-2
Step 2: Set the Speed and Rotor,3-3
Step 3: Set the Acceleration and Deceleration Profiles,3-5

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Contents
Step 4: Set the Time,3-6
Step 5: Set the Temperature,3-7
Step 6: Start the Run,3-9
Zonal and Continuous Flow Operation,3-10
Preparing for the Run,3-11
Starting the Run,3-11
Loading the Sample,3-12
Running the Sample,3-12
Unloading the Sample,3-12
Stopping the Run,3-13
Finishing the Run,3-13
CHAPTER 4: Configuration,4-1
Managing Your Network ,4-1
Setting Up the Network ,4-1
Selecting a Printer,4-2
Setting Up Email ,4-3
Setting Up VNC,4-4
Enabling API,4-4
Managing Users,4-5
Adding Users,4-5
Requiring Login,4-6
PIN Expiration and Logout Timer,4-7
User Options,4-8
Managing Rotors,4-8
Adding Rotors,4-9
Requiring Rotor Selection,4-9
Managing Reports,4-10
User Access,4-10
Filter Data,4-10
Graph Data,4-12
Print Data,4-13
Export Data,4-14
Auto Print and Auto Export Run History Data,4-16
To enable Auto Print: ,4-16
To enable Auto Export:,4-16
Run Comments,4-17
E-Signature,4-18
CHAPTER 5: Programs,5-1
Creating Programs,5-1
Running Programs,5-5
Editing Programs,5-6
Deleting Programs,5-6

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Contents
CHAPTER 6: Calculations ,6-1
Using Calculations,6-1
Calculations Page,6-1
Reduce Rotor Speed For Dense Solutions,6-3
Reduce Rotor Speed For Precipitating Solutions,6-4
Sedimentation Coefficient From Run Data,6-5
Sedimentation Coefficient From Molecular Mass ,6-7
Pelleting Time,6-8
Refractive Index ,6-9
Concentration Measures ,6-10
CHAPTER 7: Simulations ,7-1
Using Simulations,7-1
Simulations Page,7-1
ESP RNA Pelleting Best Run,7-3
ESP RNA Pelleting Fast Run,7-5
ESP Pelleting Run,7-6
ESP Rate Zonal Run,7-8
ESP Plasmid Run,7-9
Substitute Rotor Run,7-11
CHAPTER 8: Functional Pages,8-1
Home Page,8-3
Home Page (Run in Progress),8-5
Set Speed Page,8-6
Select Rotor and Labware Page (Library),8-7
Set Time Page,8-8
Delay Start Page,8-9
Set Speed ω²t Time Page,8-10
Set Temperature Page,8-11
Set Acceleration/Deceleration Profiles Page,8-12
Example Acceleration and Deceleration Profiles,8-13
Login Page,8-14
Select Program Page,8-15
New/Edit/View Program Page,8-16
Select Rotor and Labware Page (Catalog),8-18
New/Edit Step Page,8-19
Program Log Page,8-20
Export Page,8-21
Authorize Users Page,8-22